Pub Date : 2023-09-25DOI: 10.2174/0122103155261774230920033831
Yuan Yuan Wu, Ling Ling, Angge lima, Du Lan, Ter gel, Ya Nan Gao, Wen Cheng Cai, Shan Tong, Huan Wang
background: Gastroduodenal ulcer is one of the common global gastrointestinal diseases. Sulongga-4 is a traditional Mongolian medicine prescription used for the treatment of gastroduodenal ulcer. However, molecular mechanism underlying the therapeutic effect of Sulongga-4 on gastroduodenal ulcer in Proteome has not been clarified. objective: Explore the molecular mechanism behind the therapeutic efficacy of traditional Mongolian medicine Sulongga-4 on the pyloric ligation-induced gastroduodenal ulcer in rats method: Gastroduodenal was induced by pyloric ligation in rats. The pathological changes of gastric and duodenal tissues were observed first, and then the serum levels of AST and ALT were measured. The significantly different proteins were verified by western blot and qRT-PCR. result: The proteomics results showed that the Mongolian medicine Sulongga-4 might act on pyloric ligation-induced gastroduodenal ulcer through differentially expressing several proteins including RPL35, RPL37 and LOC102548628 in gastric tissue, as well as Serpin b1a, Serpin b6a and Vtn in duodenal tissue via regulating ribosome, alcoholism and amoebiasis, complement and coagulation cascades pathways. In addition, the changes of serum AST and ALT levels in rats showed that gastroduodenal ulcer was associated with liver injury. conclusion: Sulongga-4 shows robust therapeutic effect against gastric duodenal ulcer. And this therapeutic effect is mainly associated with pathways of Ribosome, Alcoholism in gastric tissue, and Amoebiasis, Complement and coagulation cascades in duodenal tissue.
{"title":"Proteome-wide Profiling Reveals Molecular Mechanism Underlying the Therapeutic Effect of Mongolian Medicine Sulongga-4 on Pyloric Ligationinduced Gastroduodenal Ulcer in Rats","authors":"Yuan Yuan Wu, Ling Ling, Angge lima, Du Lan, Ter gel, Ya Nan Gao, Wen Cheng Cai, Shan Tong, Huan Wang","doi":"10.2174/0122103155261774230920033831","DOIUrl":"https://doi.org/10.2174/0122103155261774230920033831","url":null,"abstract":"background: Gastroduodenal ulcer is one of the common global gastrointestinal diseases. Sulongga-4 is a traditional Mongolian medicine prescription used for the treatment of gastroduodenal ulcer. However, molecular mechanism underlying the therapeutic effect of Sulongga-4 on gastroduodenal ulcer in Proteome has not been clarified. objective: Explore the molecular mechanism behind the therapeutic efficacy of traditional Mongolian medicine Sulongga-4 on the pyloric ligation-induced gastroduodenal ulcer in rats method: Gastroduodenal was induced by pyloric ligation in rats. The pathological changes of gastric and duodenal tissues were observed first, and then the serum levels of AST and ALT were measured. The significantly different proteins were verified by western blot and qRT-PCR. result: The proteomics results showed that the Mongolian medicine Sulongga-4 might act on pyloric ligation-induced gastroduodenal ulcer through differentially expressing several proteins including RPL35, RPL37 and LOC102548628 in gastric tissue, as well as Serpin b1a, Serpin b6a and Vtn in duodenal tissue via regulating ribosome, alcoholism and amoebiasis, complement and coagulation cascades pathways. In addition, the changes of serum AST and ALT levels in rats showed that gastroduodenal ulcer was associated with liver injury. conclusion: Sulongga-4 shows robust therapeutic effect against gastric duodenal ulcer. And this therapeutic effect is mainly associated with pathways of Ribosome, Alcoholism in gastric tissue, and Amoebiasis, Complement and coagulation cascades in duodenal tissue.","PeriodicalId":56153,"journal":{"name":"Natural Products Journal","volume":"39 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135863062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-31DOI: 10.2174/2210315514666230831151545
J. Dash, G. Pattnaik, Goutam Ghosh, Goutam Rath, B. Kar
Diabetes mellitus is a chronic metabolic disorder affecting global public health. Since ancient, natural-based compounds are widely used for multiple indications of diabetes. The natural-based (-)-Epicatechin has enormous biological functions including antioxidant and anti-inflammatory activities. This review mainly focuses on the importance of epicatechin in the control of pathogenesis involved in diabetic mellitus. Additionally, its possible mechanisms involved in beta cell regeneration, insulin secretion, and insulin sensitivity. The present article explored the potential antioxidant, mitochondrial protection, and anti-inflammatory properties using the preclinical and clinical model, and also established the role of (-)-Epicatechin in the mitigation of diabetic-associated complications. Lastly, the article mentioned the limitation of the use of epicatechin. This will provide new insight to budding scientists for the development of novel bioactive-based pharmaceuticals for the management of diabetic mellitus.
{"title":"An overview of the therapeutic efficacy of (-)-Epicatechin in the management of diabetes mellitus","authors":"J. Dash, G. Pattnaik, Goutam Ghosh, Goutam Rath, B. Kar","doi":"10.2174/2210315514666230831151545","DOIUrl":"https://doi.org/10.2174/2210315514666230831151545","url":null,"abstract":"\u0000\u0000Diabetes mellitus is a chronic metabolic disorder affecting global public health. Since ancient, natural-based compounds are widely used for multiple indications of diabetes.\u0000\u0000\u0000\u0000The natural-based (-)-Epicatechin has enormous biological functions including antioxidant and anti-inflammatory activities. This review mainly focuses on the importance of epicatechin in the control of pathogenesis involved in diabetic mellitus. Additionally, its possible mechanisms involved in beta cell regeneration, insulin secretion, and insulin sensitivity.\u0000\u0000\u0000\u0000The present article explored the potential antioxidant, mitochondrial protection, and anti-inflammatory properties using the preclinical and clinical model, and also established the role of (-)-Epicatechin in the mitigation of diabetic-associated complications.\u0000Lastly, the article mentioned the limitation of the use of epicatechin.\u0000\u0000\u0000\u0000This will provide new insight to budding scientists for the development of novel bioactive-based pharmaceuticals for the management of diabetic mellitus.\u0000","PeriodicalId":56153,"journal":{"name":"Natural Products Journal","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49631474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-23DOI: 10.2174/2210315514666230823094450
E. Poulios, Georgios K. Vasios, Evmorfia Psara, Georgios Antasouras, Maria Gialeli, Eleni Pavlidou, A. Tsantili-Kakoulidou, A. Troumbis, C. Giaginis
Medicinal plants and herbs constitute rich sources of flavoring, and aromatic compounds, namely phytochemicals, which have many positive impacts on human health, such as antioxidant, anticancer, antimicrobial, anti-inflammatory, cardioprotective, and neuroprotective properties. Such bioactive compounds may be considered lead compounds that can be introduced in the drug design process to obtain novel drug candidates with better bioavailability and therapeutic efficiency. The Islands of the North Aegean have rich biodiversity and many medicinal plants and herbs with multiple health benefits. This study aims to summarize and scrutinize the antioxidant activity of medicinal plants and herbs of the North Aegean islands. A thorough search of the existing literature was performed in the most accurate scientific databases, using a set of effective and relative keywords and including only clinical human studies written in English. Several clinical studies have highlighted the potential antioxidant activity of phytochemicals from plants such as St. John's wort, chamomile, rosemary, spearmint, mastiha, mountain tea, oregano, sage, and thyme, as measured in the blood and saliva of human individuals, after administration of extracts and solutions of these plants. Decreased levels of oxidized lipoproteins, increased activity of antioxidant enzymes, and an enhanced total antioxidant capacity were observed. Current clinical trials remain still limited, and an enhanced series is needed with a better methodology design, larger sample size, longer intervention periods, using controlled groups and randomization to unravel the antioxidant activity of these North Aegean endemic plants in human individuals.
{"title":"Antioxidant activity of medicinal plants and herbs of North Aegean, Greece: Current clinical evidence and future perspectives","authors":"E. Poulios, Georgios K. Vasios, Evmorfia Psara, Georgios Antasouras, Maria Gialeli, Eleni Pavlidou, A. Tsantili-Kakoulidou, A. Troumbis, C. Giaginis","doi":"10.2174/2210315514666230823094450","DOIUrl":"https://doi.org/10.2174/2210315514666230823094450","url":null,"abstract":"\u0000\u0000Medicinal plants and herbs constitute rich sources of flavoring, and aromatic compounds, namely phytochemicals, which have many positive impacts on human health, such as antioxidant, anticancer, antimicrobial, anti-inflammatory, cardioprotective, and neuroprotective properties. Such bioactive compounds may be considered lead compounds that can be introduced in the drug design process to obtain novel drug candidates with better bioavailability and therapeutic efficiency. The Islands of the North Aegean have rich biodiversity and many medicinal plants and herbs with multiple health benefits.\u0000\u0000\u0000\u0000This study aims to summarize and scrutinize the antioxidant activity of medicinal plants and herbs of the North Aegean islands.\u0000\u0000\u0000\u0000A thorough search of the existing literature was performed in the most accurate scientific databases, using a set of effective and relative keywords and including only clinical human studies written in English.\u0000\u0000\u0000\u0000Several clinical studies have highlighted the potential antioxidant activity of phytochemicals from plants such as St. John's wort, chamomile, rosemary, spearmint, mastiha, mountain tea, oregano, sage, and thyme, as measured in the blood and saliva of human individuals, after administration of extracts and solutions of these plants. Decreased levels of oxidized lipoproteins, increased activity of antioxidant enzymes, and an enhanced total antioxidant capacity were observed.\u0000\u0000\u0000\u0000Current clinical trials remain still limited, and an enhanced series is needed with a better methodology design, larger sample size, longer intervention periods, using controlled groups and randomization to unravel the antioxidant activity of these North Aegean endemic plants in human individuals.\u0000","PeriodicalId":56153,"journal":{"name":"Natural Products Journal","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43025420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-16DOI: 10.2174/2210315514666230816141802
R. Pal, Y. Pal, Saranya Punniyakotti, Deepti Katiyar, P. Kumari
Hesperidin is one of the most well-known, naturally occurring flavonoids with antioxidant and anti-inflammatory effect, occurring in high concentrations in citrus fruits. Citrus fruits and juices are widely consumed worldwide and are readily available dietary sources for their intake. Its use has been associated with a great number of health benefits, including antioxidant, antibacterial, antimicrobial, anti-inflammatory and anti-carcinogenic properties. Due to its biological activities, hesperidin is often used in the food, cosmetic and pharmaceutical industries. Supplements containing hesperidin, alone or in combination with other citrus bioflavonoids, are commercially available. The utilization of hesperidin can be used for the production of novel nutraceuticals or for the improvement of older ones. This piece of review aims to discuss the various diversified aspects of the naturally occurring bioflavonoid hesperidin.
{"title":"Hesperidin: Diversified Prospects of Naturally Occurring Bioflavonoid","authors":"R. Pal, Y. Pal, Saranya Punniyakotti, Deepti Katiyar, P. Kumari","doi":"10.2174/2210315514666230816141802","DOIUrl":"https://doi.org/10.2174/2210315514666230816141802","url":null,"abstract":"\u0000\u0000Hesperidin is one of the most well-known, naturally occurring flavonoids with antioxidant and anti-inflammatory effect, occurring in high concentrations in citrus fruits. Citrus fruits and juices are widely consumed worldwide and are readily available dietary sources for their intake. Its use has been associated with a great number of health benefits, including antioxidant, antibacterial, antimicrobial, anti-inflammatory and anti-carcinogenic properties. Due to its biological activities, hesperidin is often used in the food, cosmetic and pharmaceutical industries. Supplements containing hesperidin, alone or in combination with other citrus bioflavonoids, are commercially available. The utilization of hesperidin can be used for the production of novel nutraceuticals or for the improvement of older ones. This piece of review aims to discuss the various diversified aspects of the naturally occurring bioflavonoid hesperidin.\u0000","PeriodicalId":56153,"journal":{"name":"Natural Products Journal","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44749875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-16DOI: 10.2174/2210315514666230816095321
S. Bose, Gurleen Kaur, Tanushka Kataria, Ankit Tyagi, Keshav Singla, Sakshi Sharma, Silpa Ghosh, C. Jha
Cancer is one of the leading causes of death worldwide. Therapy for cancer has remained a challenge for a very long period of time, though developments of various treatment strategies have been introduced. However, Curcuma longa L (Turmeric) attains major attention to cure cancer due to its phytoconstituents which can be used in the treatment of various cancers. Due to the wide availability and minimal adverse effects, curcumin has become a source of significant interest in research for cancer treatment which can lead to heavy cost cut off in the treatment. In this review article, the anti-cancer properties of curcumin in the treatment of cancer are highlighted. Scientists from all over the world have been interested in curcumin, a polyphenol isolated from Curcuma longa, for its biological properties, the most well-known of which is its ability to fight cancer. It is one of the most promising classes of bioactive natural substances, particularly in the treatment of various cancer types. Curcumin demonstrates anticancer properties. To overcome the limitation of curcumin numerous studies, analysis, and research has been already completed and many are still going on. The use of curcumin either alone or in combination might alter the treatment of cancer
癌症是世界范围内导致死亡的主要原因之一。癌症的治疗在很长一段时间内仍然是一个挑战，尽管已经介绍了各种治疗策略的发展。然而，姜黄(Curcuma longa L，姜黄)由于其植物成分可用于治疗各种癌症而引起了人们对癌症治疗的关注。由于姜黄素的广泛可用性和最小的不良反应，姜黄素已成为癌症治疗研究的重要兴趣来源，这可能导致治疗成本的大幅削减。本文就姜黄素在癌症治疗中的抗癌作用作一综述。姜黄素是一种从姜黄中分离出来的多酚，世界各地的科学家都对它的生物学特性很感兴趣，其中最著名的是它的抗癌能力。它是最有前途的一类生物活性天然物质，特别是在治疗各种癌症类型。姜黄素具有抗癌特性。为了克服姜黄素的局限性，许多研究、分析和研究已经完成，许多研究仍在进行中。单独或联合使用姜黄素可能会改变癌症的治疗方法
{"title":"Curcumin as a potential phytoconstituent used for cancer treatment: An Overview","authors":"S. Bose, Gurleen Kaur, Tanushka Kataria, Ankit Tyagi, Keshav Singla, Sakshi Sharma, Silpa Ghosh, C. Jha","doi":"10.2174/2210315514666230816095321","DOIUrl":"https://doi.org/10.2174/2210315514666230816095321","url":null,"abstract":"\u0000\u0000Cancer is one of the leading causes of death worldwide. Therapy for cancer has remained a challenge for a very long period of time, though developments of various treatment strategies have been introduced. However, Curcuma longa L (Turmeric) attains major attention to cure cancer due to its phytoconstituents which can be used in the treatment of various cancers. Due to the wide availability and minimal adverse effects, curcumin has become a source of significant interest in research for cancer treatment which can lead to heavy cost cut off in the treatment.\u0000\u0000\u0000\u0000In this review article, the anti-cancer properties of curcumin in the treatment of cancer are highlighted.\u0000\u0000\u0000\u0000Scientists from all over the world have been interested in curcumin, a polyphenol isolated from Curcuma longa, for its biological properties, the most well-known of which is its ability to fight cancer. It is one of the most promising classes of bioactive natural substances, particularly in the treatment of various cancer types. Curcumin demonstrates anticancer properties. To overcome the limitation of curcumin numerous studies, analysis, and research has been already completed and many are still going on. The use of curcumin either alone or in combination might alter the treatment of cancer\u0000","PeriodicalId":56153,"journal":{"name":"Natural Products Journal","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42632114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-08DOI: 10.2174/2210315514666230808142940
Danival José de Souza, Márcio Silva Melo, Cynthia Lhourrana Santos Silva, Amanda Caroline Teles Tenório, Bruno Silva Melo, Rone da Silva Barbosa, R. Sarmento, Juliana Cristina Holzbach, Állefe Barbosa Cruz, Isabele Rodrigues Nascimento
The active secondary metabolites of various Kalanchoe species are promising for controlling insects and other agricultural pests. This study aimed to evaluate the mortality and immune responses of the leafcutter ant Atta sexdens (L.) after treatment with Kalanchoe ×laetivirens (Desc.) aqueous extract. The effects on an aquatic organism used as a bioindicator, Chironomus xanthus (Rempel), were also evaluated. Different K. ×laetivirens concentrations were prepared by dilution in honey to assess the toxicity and determine the median lethal concentration (LC50) for A. sexdens workers. The haemocytes of workers treated with a sublethal concentration (0.005 g/mL) were counted. The plant extracts induced high mortality in A. sexdens workers. All concentrations, except 0.001 g/mL, increased mortality rates. The sublethal concentration negatively affected the immune system of A. sexdens workers 48 and 72 h after ingestion. The flavonoids 4″″-acetylsagittatin A, sagittatin A and the nucleosides uridine and adenosine were identified. The LC50 of the K. ×laetivirens (128.1 mg /L) was determined for the non-target organism, C. xanthus. These natural compounds can be used in future research to plan alternative control strategies for leafcutter ants in forests and other agroecosystems.
{"title":"Flavonoids from Kalanchoe ×laetivirens Impair Survival and Immunity of Atta sexdens (Hymenoptera: Formicidae)","authors":"Danival José de Souza, Márcio Silva Melo, Cynthia Lhourrana Santos Silva, Amanda Caroline Teles Tenório, Bruno Silva Melo, Rone da Silva Barbosa, R. Sarmento, Juliana Cristina Holzbach, Állefe Barbosa Cruz, Isabele Rodrigues Nascimento","doi":"10.2174/2210315514666230808142940","DOIUrl":"https://doi.org/10.2174/2210315514666230808142940","url":null,"abstract":"\u0000\u0000The active secondary metabolites of various Kalanchoe species are promising for controlling insects and other agricultural pests.\u0000\u0000\u0000\u0000This study aimed to evaluate the mortality and immune responses of the leafcutter ant Atta sexdens (L.) after treatment with Kalanchoe ×laetivirens (Desc.) aqueous extract. The effects on an aquatic organism used as a bioindicator, Chironomus xanthus (Rempel), were also evaluated.\u0000\u0000\u0000\u0000Different K. ×laetivirens concentrations were prepared by dilution in honey to assess the toxicity and determine the median lethal concentration (LC50) for A. sexdens workers. The haemocytes of workers treated with a sublethal concentration (0.005 g/mL) were counted.\u0000\u0000\u0000\u0000The plant extracts induced high mortality in A. sexdens workers. All concentrations, except 0.001 g/mL, increased mortality rates. The sublethal concentration negatively affected the immune system of A. sexdens workers 48 and 72 h after ingestion. The flavonoids 4″″-acetylsagittatin A, sagittatin A and the nucleosides uridine and adenosine were identified. The LC50 of the K. ×laetivirens (128.1 mg /L) was determined for the non-target organism, C. xanthus.\u0000\u0000\u0000\u0000These natural compounds can be used in future research to plan alternative control strategies for leafcutter ants in forests and other agroecosystems.\u0000","PeriodicalId":56153,"journal":{"name":"Natural Products Journal","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44440577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-08DOI: 10.2174/2210315514666230808153921
Tran Thanh Men, Huynh Hong Phien, Nguyen Hoang Son, Truong Thi Phuong Thao, Nguyen Thi Bich Nhu, Nguyen Thi To Uyen, Tran Quang De, Do Tan Khang
Background: Peperomia pellucida is a medicinal and vegetable plant used worldwide, representing a multi-purpose vegetable with applications in the pharmaceutical, food and cosmetic industries. Objective: This study evaluated the antioxidant, antibacterial, antifungal, anti-inflammatory, and antidiabetic potential of fractional extracts from P. pellucida plant derived from Can Tho City, Vietnam. Methods: Four fractional extracts were prepared using different polarity solvents (hexane, dichloromethane, ethyl acetate) and used to determine the best extract for each biological property. The fractions’ total alkaloid, phenolic, and flavonoid content were observed. The four extracts were evaluated for their potential bioactivities: antioxidant, antibacterial, antifungal, anti-inflammatory, and antidiabetic. Results: Correspond with the hexane, dichloromethane, ethyl acetate, and aqueous fractions, the total content of alkaloids was determined to be 255 ± 23.8; 157 ± 14.0; 219 ± 6.55; 221 ± 6.23 (mg AE/g extract), the total phenolic content was 112 ± 3.34; 141 ± 1.77; 234 ± 29.5; 123 ± 5.04 (mg GAE/g extract), whereas the total content of flavonoids was 423 ± 22.6; 169 ± 6.30; 1839 ± 18.8; 173 ± 22.6 (mg QE/g extract), respectively. The ethyl acetate fraction gave the best efficiency in DPPH, ABTS, iron reduction, and TAC methods (IC50 = 334 ± 2.10 µg/mL; 51.4 ± 0.41 µg/mL; 79.1 ± 0.40 µg/mL; and 83.0 ± 0.17 µg/mL, respectively). Antibacterial activity was investigated on 5 strains of Bacillus cereus, Escherichia coli, Staphylococcus aureus, Salmonella typhimunum, Pseudomonas aeruginosa; the results showed that the extracts were resistant to 5 strains of bacteria, especially best resistant in 2 fractions of ethyl acetate and aqueous. The minimum inhibitory concentration (MIC) value ranged from 0.5 to 32 mg/mL, while the minimum bactericidal concentration (MBC) value ranged from 16 to 64 mg/mL. The best anti-inflammatory activity was ethyl acetate with an IC50 value of 216.7 ± 7.2 µg/mL, close to that of Diclofenac at 205.4 ± 0.5. The antidiabetic activity was investigated based on the ability to inhibit α-amylase and α-glucosidase enzymes. The results showed that the best α-amylase inhibitors were hexane and dichloromethane (IC50 = 208.83 ± 2.41 and 191.60 ± 1.27 µg/mL, respectively), roughly equal to the acarbose (155.68 ± 2.59 µg/mL). The best α-glucosidase inhibitory fraction was ethyl acetate (IC50 of 157.04 ± 0.23 µg/mL), close to that of acarbose (116.45 ± 0.21 µg/mL). Conclusion: Fractional extracts from P. pellucida distributed in gardens of Can Tho City, Vietnam, contain potential antioxidant, antibacterial, antifungal, anti-inflammatory, and antidiabetic bioactive compounds.
{"title":"Chemical composition and biological activities of the extracts of Peperomia pellucida fractions","authors":"Tran Thanh Men, Huynh Hong Phien, Nguyen Hoang Son, Truong Thi Phuong Thao, Nguyen Thi Bich Nhu, Nguyen Thi To Uyen, Tran Quang De, Do Tan Khang","doi":"10.2174/2210315514666230808153921","DOIUrl":"https://doi.org/10.2174/2210315514666230808153921","url":null,"abstract":"Background: Peperomia pellucida is a medicinal and vegetable plant used worldwide, representing a multi-purpose vegetable with applications in the pharmaceutical, food and cosmetic industries. Objective: This study evaluated the antioxidant, antibacterial, antifungal, anti-inflammatory, and antidiabetic potential of fractional extracts from P. pellucida plant derived from Can Tho City, Vietnam. Methods: Four fractional extracts were prepared using different polarity solvents (hexane, dichloromethane, ethyl acetate) and used to determine the best extract for each biological property. The fractions’ total alkaloid, phenolic, and flavonoid content were observed. The four extracts were evaluated for their potential bioactivities: antioxidant, antibacterial, antifungal, anti-inflammatory, and antidiabetic. Results: Correspond with the hexane, dichloromethane, ethyl acetate, and aqueous fractions, the total content of alkaloids was determined to be 255 ± 23.8; 157 ± 14.0; 219 ± 6.55; 221 ± 6.23 (mg AE/g extract), the total phenolic content was 112 ± 3.34; 141 ± 1.77; 234 ± 29.5; 123 ± 5.04 (mg GAE/g extract), whereas the total content of flavonoids was 423 ± 22.6; 169 ± 6.30; 1839 ± 18.8; 173 ± 22.6 (mg QE/g extract), respectively. The ethyl acetate fraction gave the best efficiency in DPPH, ABTS, iron reduction, and TAC methods (IC50 = 334 ± 2.10 µg/mL; 51.4 ± 0.41 µg/mL; 79.1 ± 0.40 µg/mL; and 83.0 ± 0.17 µg/mL, respectively). Antibacterial activity was investigated on 5 strains of Bacillus cereus, Escherichia coli, Staphylococcus aureus, Salmonella typhimunum, Pseudomonas aeruginosa; the results showed that the extracts were resistant to 5 strains of bacteria, especially best resistant in 2 fractions of ethyl acetate and aqueous. The minimum inhibitory concentration (MIC) value ranged from 0.5 to 32 mg/mL, while the minimum bactericidal concentration (MBC) value ranged from 16 to 64 mg/mL. The best anti-inflammatory activity was ethyl acetate with an IC50 value of 216.7 ± 7.2 µg/mL, close to that of Diclofenac at 205.4 ± 0.5. The antidiabetic activity was investigated based on the ability to inhibit α-amylase and α-glucosidase enzymes. The results showed that the best α-amylase inhibitors were hexane and dichloromethane (IC50 = 208.83 ± 2.41 and 191.60 ± 1.27 µg/mL, respectively), roughly equal to the acarbose (155.68 ± 2.59 µg/mL). The best α-glucosidase inhibitory fraction was ethyl acetate (IC50 of 157.04 ± 0.23 µg/mL), close to that of acarbose (116.45 ± 0.21 µg/mL). Conclusion: Fractional extracts from P. pellucida distributed in gardens of Can Tho City, Vietnam, contain potential antioxidant, antibacterial, antifungal, anti-inflammatory, and antidiabetic bioactive compounds.","PeriodicalId":56153,"journal":{"name":"Natural Products Journal","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135834890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Ionizing radiation (IR) generates reactive oxygen species (ROS), which leads to oxidative stress that often leads to inflammatory responses in organisms. Objective: Trianthema portulacastrum L., a plant commonly growing in India, is rich in antioxidant phytochemicals. This is responsible for scavenging free radicals and may provide radioprotective and anti-inflammatory effects in response to ionizing radiation. Methods: The effect of T. portulacastrum extracts was studied in hepatic cells, which are susceptible to radiation-induced damage and in macrophages, which are the primary inflammatory cells of the body. Results: T. portulacastrum stem extracts showed efficient free radical scavenging activity in hepatocytes and decreased radiation-induced lipid peroxidation in cell and mitochondrial membranes. Treatment of irradiated cells with T. portulacastrum stem extracts enhanced cell viability at lower concentration and reduced cell viability at higher concentration. Treatment with low concentration of T. portulacastrum stem extract also reduced cellular ROS generation and increased the concentration of cellular anti-oxidant, glutathione. T. portulacastrum extracts also showed remarkable anti-inflammatory properties in macrophages activated by the inflammatory agonist bacterial lipopolysaccharide (LPS). The extract reduced nitric oxide (NO) production and suppressed the expression of inflammatory genes. Conclusion: Together, these observations demonstrated a potential radioprotective role of T. portulacastrum extract mediated by both its antioxidant activity on hepatic epithelial cells and its anti-inflammatory activity on immune cells.
{"title":"Antioxidant and Anti-inflammatory Activities Mediate the Radioprotective Effect of <i>Trianthema portulacastrum L</i>. Extracts","authors":"Uttam Das, Tanmay Saha, Reshma Kumari Sharma, Dharmendra Kumar Maurya, Partho Sarothi Ray, Subir Kumar Das","doi":"10.2174/2210315512666220627154721","DOIUrl":"https://doi.org/10.2174/2210315512666220627154721","url":null,"abstract":"Background: Ionizing radiation (IR) generates reactive oxygen species (ROS), which leads to oxidative stress that often leads to inflammatory responses in organisms. Objective: Trianthema portulacastrum L., a plant commonly growing in India, is rich in antioxidant phytochemicals. This is responsible for scavenging free radicals and may provide radioprotective and anti-inflammatory effects in response to ionizing radiation. Methods: The effect of T. portulacastrum extracts was studied in hepatic cells, which are susceptible to radiation-induced damage and in macrophages, which are the primary inflammatory cells of the body. Results: T. portulacastrum stem extracts showed efficient free radical scavenging activity in hepatocytes and decreased radiation-induced lipid peroxidation in cell and mitochondrial membranes. Treatment of irradiated cells with T. portulacastrum stem extracts enhanced cell viability at lower concentration and reduced cell viability at higher concentration. Treatment with low concentration of T. portulacastrum stem extract also reduced cellular ROS generation and increased the concentration of cellular anti-oxidant, glutathione. T. portulacastrum extracts also showed remarkable anti-inflammatory properties in macrophages activated by the inflammatory agonist bacterial lipopolysaccharide (LPS). The extract reduced nitric oxide (NO) production and suppressed the expression of inflammatory genes. Conclusion: Together, these observations demonstrated a potential radioprotective role of T. portulacastrum extract mediated by both its antioxidant activity on hepatic epithelial cells and its anti-inflammatory activity on immune cells.","PeriodicalId":56153,"journal":{"name":"Natural Products Journal","volume":"48 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134951521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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