Pub Date : 2019-01-17DOI: 10.4236/OJBIPHY.2019.91005
Lilia Narinyan, S. Ayrapetyan
Previously we have shown that 4 Hz and 8 Hz EMF exposures have depressing effect on the thermodynamic activity of water, which decreases peroxide formation. It has also been shown that 4 Hz EMF-treated physiological solution modulates the growth and development of microbes and heart muscle contractility, but 8 Hz EMF has pronounced inhibitory effect on bacterial growth and development. Therefore, in order to elucidate the possible mechanism of 4 Hz and 8 Hz EMF effects on heart muscle function, in the present work the effects of 4 Hz and 8 Hz EMF exposures on heart muscle tissue hydration, the sensitivity of 4 Hz and 8 Hz EMF-induced tissue hydration to 10−4 M ouabain (Na+/K+ pump inhibition) and 10−9 M ouabain (activation of intracellular signaling system) as well as the effects of 4 Hz and 8 Hz EMF exposures on the number of Na+/K+ pump units in the membrane of both young and old rats have been studied. The obtained data allow us to suggest that 8 Hz EMF exposure has more pronounced age-dependent modulation effect on tissue hydration of heart muscle than 4 Hz EMF and this effect is sensitive to Na+/K+ pump activity and intracellular signaling system.
{"title":"Age-Dependent Comparative Study of 4 Hz and 8 Hz EMF Exposure on Heart Muscle Tissue Hydration of Rats","authors":"Lilia Narinyan, S. Ayrapetyan","doi":"10.4236/OJBIPHY.2019.91005","DOIUrl":"https://doi.org/10.4236/OJBIPHY.2019.91005","url":null,"abstract":"Previously we have shown that 4 Hz and 8 Hz EMF exposures have depressing effect on the thermodynamic activity of water, which decreases peroxide formation. It has also been shown that 4 Hz EMF-treated physiological solution modulates the growth and development of microbes and heart muscle contractility, but 8 Hz EMF has pronounced inhibitory effect on bacterial growth and development. Therefore, in order to elucidate the possible mechanism of 4 Hz and 8 Hz EMF effects on heart muscle function, in the present work the effects of 4 Hz and 8 Hz EMF exposures on heart muscle tissue hydration, the sensitivity of 4 Hz and 8 Hz EMF-induced tissue hydration to 10−4 M ouabain (Na+/K+ pump inhibition) and 10−9 M ouabain (activation of intracellular signaling system) as well as the effects of 4 Hz and 8 Hz EMF exposures on the number of Na+/K+ pump units in the membrane of both young and old rats have been studied. The obtained data allow us to suggest that 8 Hz EMF exposure has more pronounced age-dependent modulation effect on tissue hydration of heart muscle than 4 Hz EMF and this effect is sensitive to Na+/K+ pump activity and intracellular signaling system.","PeriodicalId":59528,"journal":{"name":"生物物理学期刊(英文)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45345134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-04DOI: 10.4236/ojbiphy.2019.91004
G. Szigeti, A. Szász
Biosystems are complex. Their physiology is well-controlled with various negative feedback signals and processes, it describes by opposite interfering effects which are characterized in the Eastern philosophy by Yin-Yang (Y-Y) pairs. Y-Y pairs could be described by the promoter-suppressor pairs in a wide range of physiologic signals creating the homeostasis of the complex system. This type of control appears as fluctuations from the average (mean) value of the signal. The mean carries an ineluctable fluctuation (called pink-noise or 1/f noise). All signals in homeostasis have equal entropy (SE = 1.8), which is the character of the complex equilibrium. The various controlling opposite signals (Y-Y) have different time-scales which change by aging. The processes with smaller time-scale are degraded by aging, but the pink-noise ensures that the deviations of the signals of the healthy homeostatic system remain constant. Meridians are connected to the general transport systems that combined the material and the information transport with the considerable transport networks, like blood, lymph, nerve, cell-junctions, mesenchymal “ground substance” cytoskeletons. The meridians in this meaning only virtual line averaged from multiple realized paths to connect two acupuncture points by the material, energy and information transport processes. The meridian network is designed by various coupling points (acupoints), which could be perturbed by actuating stimulus. Our objective is to describe the meridian system from complexity point of view.
{"title":"Fluctuations Hypothesize the New Explanation of Meridians in Living Systems","authors":"G. Szigeti, A. Szász","doi":"10.4236/ojbiphy.2019.91004","DOIUrl":"https://doi.org/10.4236/ojbiphy.2019.91004","url":null,"abstract":"Biosystems are complex. Their physiology is well-controlled with various negative feedback signals and processes, it describes by opposite interfering effects which are characterized in the Eastern philosophy by Yin-Yang (Y-Y) pairs. Y-Y pairs could be described by the promoter-suppressor pairs in a wide range of physiologic signals creating the homeostasis of the complex system. This type of control appears as fluctuations from the average (mean) value of the signal. The mean carries an ineluctable fluctuation (called pink-noise or 1/f noise). All signals in homeostasis have equal entropy (SE = 1.8), which is the character of the complex equilibrium. The various controlling opposite signals (Y-Y) have different time-scales which change by aging. The processes with smaller time-scale are degraded by aging, but the pink-noise ensures that the deviations of the signals of the healthy homeostatic system remain constant. Meridians are connected to the general transport systems that combined the material and the information transport with the considerable transport networks, like blood, lymph, nerve, cell-junctions, mesenchymal “ground substance” cytoskeletons. The meridians in this meaning only virtual line averaged from multiple realized paths to connect two acupuncture points by the material, energy and information transport processes. The meridian network is designed by various coupling points (acupoints), which could be perturbed by actuating stimulus. Our objective is to describe the meridian system from complexity point of view.","PeriodicalId":59528,"journal":{"name":"生物物理学期刊(英文)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46195037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.4236/ojbiphy.2019.91003
David T. Heider, J. van Hemmen
Whereas for smaller animals the eardrums are well-characterized as excitable membranes or drums, some animals such as several archosaurs feature, as a first approximation, a rather stiff elastic shell supported by an elastic ring. Mathematically, the theory of plates and shells is applicable but its governing equations overly complicate the modeling. Here the notion of tympanic structure is introduced as a generalization of “ordinary” tympanic membranes so as to account for sound perception as it occurs in archosaurs, such as birds and crocodilians. A mathematical model for the tympanic structure in many archosaurs called two-spring model implements this notion. The model is exactly soluble and solutions are presented in closed form and as a series expansion. Special emphasis is put onto offering an easy-to-apply model for describing experiments and performing numerical studies. The analytic treatment is supplemented by a discussion of the applicability of the two-spring model in auditory research. An elasticity-theoretic perspective of the two-spring model is given in the Appendix.
{"title":"Effective-Spring Model of Tympanic Response in Archosaurs","authors":"David T. Heider, J. van Hemmen","doi":"10.4236/ojbiphy.2019.91003","DOIUrl":"https://doi.org/10.4236/ojbiphy.2019.91003","url":null,"abstract":"Whereas for smaller animals the eardrums are well-characterized as excitable membranes or drums, some animals such as several archosaurs feature, as a first approximation, a rather stiff elastic shell supported by an elastic ring. Mathematically, the theory of plates and shells is applicable but its governing equations overly complicate the modeling. Here the notion of tympanic structure is introduced as a generalization of “ordinary” tympanic membranes so as to account for sound perception as it occurs in archosaurs, such as birds and crocodilians. A mathematical model for the tympanic structure in many archosaurs called two-spring model implements this notion. The model is exactly soluble and solutions are presented in closed form and as a series expansion. Special emphasis is put onto offering an easy-to-apply model for describing experiments and performing numerical studies. The analytic treatment is supplemented by a discussion of the applicability of the two-spring model in auditory research. An elasticity-theoretic perspective of the two-spring model is given in the Appendix.","PeriodicalId":59528,"journal":{"name":"生物物理学期刊(英文)","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70618315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.4236/ojbiphy.2019.91001
A. Atanasov
Experimental and theoretical arguments have been described in favor of the hypothesis that the nasal cycle reversals of the nostril’s airflow occurs only in the consciousness states of the brain (during awake/wake state, REM state and during dreams in St.1, 2 of the non-REM sleep). This finding gives possibility to use nasal cycle as “marker for consciousness states”. An idea for clinical application of the hypothesis is proposed.
{"title":"Nasal Cycle Dilemma: Nasal Cycle Is Associated to Brain Wake/REM States or Is Associated to Conscious State in Them?","authors":"A. Atanasov","doi":"10.4236/ojbiphy.2019.91001","DOIUrl":"https://doi.org/10.4236/ojbiphy.2019.91001","url":null,"abstract":"Experimental and theoretical arguments have been described in favor of the hypothesis that the nasal cycle reversals of the nostril’s airflow occurs only in the consciousness states of the brain (during awake/wake state, REM state and during dreams in St.1, 2 of the non-REM sleep). This finding gives possibility to use nasal cycle as “marker for consciousness states”. An idea for clinical application of the hypothesis is proposed.","PeriodicalId":59528,"journal":{"name":"生物物理学期刊(英文)","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70618249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-11-07DOI: 10.4236/OJBIPHY.2019.91002
S. Kinoshita, H. Yamada
Study of network dynamics is very active area in biological and social sciences. However, the relationship between the network structure and the attractors of the dynamics has not been fully understood yet. In this study, we numerically investigated the role of degenerate self-loops on the attractors and its basin size using the budding yeast cell-cycle network model. In the network, all self-loops negatively suppress the node (self-inhibition loops) and the attractors are only fixed points, i.e. point attractors. It is found that there is a simple division rule of the state space by removing the self-loops when the attractors consist only of point attractors. The point attractor with largest basin size is robust against the change of the self-inhibition loop. Furthermore, some limit cycles of period 2 appear as new attractor when a self-activation loop is added to the original network. It is also shown that even in that case, the point attractor with largest basin size is robust.
{"title":"Role of Self-Loop in Cell-Cycle Network of Budding Yeast","authors":"S. Kinoshita, H. Yamada","doi":"10.4236/OJBIPHY.2019.91002","DOIUrl":"https://doi.org/10.4236/OJBIPHY.2019.91002","url":null,"abstract":"Study of network dynamics is very active area in biological and social sciences. However, the relationship between the network structure and the attractors of the dynamics has not been fully understood yet. In this study, we numerically investigated the role of degenerate self-loops on the attractors and its basin size using the budding yeast cell-cycle network model. In the network, all self-loops negatively suppress the node (self-inhibition loops) and the attractors are only fixed points, i.e. point attractors. It is found that there is a simple division rule of the state space by removing the self-loops when the attractors consist only of point attractors. The point attractor with largest basin size is robust against the change of the self-inhibition loop. Furthermore, some limit cycles of period 2 appear as new attractor when a self-activation loop is added to the original network. It is also shown that even in that case, the point attractor with largest basin size is robust.","PeriodicalId":59528,"journal":{"name":"生物物理学期刊(英文)","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70618301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-09-13DOI: 10.4236/OJBIPHY.2018.84014
Daniel Maitethia Memeu, Merenga Abdallah Sarroney, C. Maina
Malaria is one of the leading killer diseases in sub-Saharan Africa. Although the disease is curable, early and accurate diagnosis is key to effective therapy. Existing malaria diagnostic techniques have low detection accuracy especially when the parasite load in the blood is low. In this paper, we report on a simple photo-thermal based technique for detection of the Plasmodium parasites’ biomarker (hemozoin) in blood smear samples. The technique has demonstrated 100% Plasmodium detection sensitivity and specificity from the ten blood smear test samples used.
{"title":"Photo-Thermal Induced Optical Scattering Modulation Sensor for Malaria Diagnosis","authors":"Daniel Maitethia Memeu, Merenga Abdallah Sarroney, C. Maina","doi":"10.4236/OJBIPHY.2018.84014","DOIUrl":"https://doi.org/10.4236/OJBIPHY.2018.84014","url":null,"abstract":"Malaria is one of the leading killer diseases in sub-Saharan Africa. Although the disease is curable, early and accurate diagnosis is key to effective therapy. Existing malaria diagnostic techniques have low detection accuracy especially when the parasite load in the blood is low. In this paper, we report on a simple photo-thermal based technique for detection of the Plasmodium parasites’ biomarker (hemozoin) in blood smear samples. The technique has demonstrated 100% Plasmodium detection sensitivity and specificity from the ten blood smear test samples used.","PeriodicalId":59528,"journal":{"name":"生物物理学期刊(英文)","volume":"8 1","pages":"185-193"},"PeriodicalIF":0.0,"publicationDate":"2018-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42772394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-09-13DOI: 10.4236/ojbiphy.2018.84015
Stephen Talba Diyong, E. Tizhe, Stephen D. Songden
The effects of electromagnetic radiation produced by a 3G cell phone (third-generation) on skin tissues and eyes were investigated in terms of histomorphological parameters. A total of 26 Wistar rats (2 weeks-old, each weighing 40 g at the time of experiment) were used. They were maintained under a control room with water and food continuously available. The animals were divided into two experimental groups: Group A (Exposed) and Group B (Control), each with 13 Wistar Rats kept inside a plexi cage. Group A was exposed to a 3G cell phone radiation while Group B the control group, was not. All animals were generally anesthetized with Ketamine injection and then decapitated. The skin tissue was excised from the dorsal area and eyes samples were taken from all the rats by enucleating of the eye balls, fixed in 10% neutral buffered formalin for a minimum of 72 hours before processing through a graded alcohol and xylene was used as a clearing agent, embedded in paraffin blocks. Tissues were sectioned at 5μm thick and routinely stained with hematoxylin/eosin. Mounted slides were examined and photographed using a light microscope. Mild to severe orthokeratotic parakeratosis was observed in the skin while eye revealed loss of striation in the sclera with necrosis of the layers of rods and cones in the retina of the exposed group. We conclude that sub chronic exposure to 3G cell phone radiation impaired the protective ability of the skin and also impaired accommodation.
{"title":"Histomorphological Changes in the Skin and Eye Induced by Sub-Chronic Exposure of Wistar Rats to 3G Cell Phone Radiation","authors":"Stephen Talba Diyong, E. Tizhe, Stephen D. Songden","doi":"10.4236/ojbiphy.2018.84015","DOIUrl":"https://doi.org/10.4236/ojbiphy.2018.84015","url":null,"abstract":"The effects of electromagnetic radiation produced by a 3G cell phone (third-generation) on skin tissues and eyes were investigated in terms of histomorphological parameters. A total of 26 Wistar rats (2 weeks-old, each weighing 40 g at the time of experiment) were used. They were maintained under a control room with water and food continuously available. The animals were divided into two experimental groups: Group A (Exposed) and Group B (Control), each with 13 Wistar Rats kept inside a plexi cage. Group A was exposed to a 3G cell phone radiation while Group B the control group, was not. All animals were generally anesthetized with Ketamine injection and then decapitated. The skin tissue was excised from the dorsal area and eyes samples were taken from all the rats by enucleating of the eye balls, fixed in 10% neutral buffered formalin for a minimum of 72 hours before processing through a graded alcohol and xylene was used as a clearing agent, embedded in paraffin blocks. Tissues were sectioned at 5μm thick and routinely stained with hematoxylin/eosin. Mounted slides were examined and photographed using a light microscope. Mild to severe orthokeratotic parakeratosis was observed in the skin while eye revealed loss of striation in the sclera with necrosis of the layers of rods and cones in the retina of the exposed group. We conclude that sub chronic exposure to 3G cell phone radiation impaired the protective ability of the skin and also impaired accommodation.","PeriodicalId":59528,"journal":{"name":"生物物理学期刊(英文)","volume":"08 1","pages":"194-203"},"PeriodicalIF":0.0,"publicationDate":"2018-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48772390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-05-29DOI: 10.4236/OJBIPHY.2018.83012
S. Sung, H. Pak, J. Kwak, Sang Weon Lee, Young Ha Kim, B. Hur, Seong Jin Jin, G. Kim
We studied the quantum dot-liposome complex (QLC), which is the giant unilamellar vesicle with quantum dots (QDs) incorporated in its lipid bilayer. A spin coating method in conjunction with the electroformation technique yielded vesicles with highly homogeneous unilamellar structure. We observed QD size dependence of the QLC formation: QLCs form with blue, green and yellow-emission QD (core radius ~1.05 nm, 1.25 nm and 1.65 nm) but not with red-emission QD (core radius ~2.5 nm). In order to explain this size dependence, we made a simple model explaining the QD size effect on QLC formation in terms of the molecular packing parameter and the lipid conformational change. This model predicts that QDs below a certain critical size (radius ≈ 1.8 nm) can stably reside in a lipid bilayer of 4 - 5 nm in thickness for Egg-PC lipids. This is consistent with our previous experimental results. In the case of red-emission QD, QD-aggregations are only observed on the fluorescent microscopy instead of QLC. We expected that the reduction of packing parameter (P) would lead to the change of specific QD radius. This prediction could be verified by our experimental observation of the shift of the specific QD size by mixing DOPG.
{"title":"Specific Radius Change of Quantum Dot inside the Lipid Bilayer by Charge Effect of Lipid Head-Group","authors":"S. Sung, H. Pak, J. Kwak, Sang Weon Lee, Young Ha Kim, B. Hur, Seong Jin Jin, G. Kim","doi":"10.4236/OJBIPHY.2018.83012","DOIUrl":"https://doi.org/10.4236/OJBIPHY.2018.83012","url":null,"abstract":"We studied the quantum dot-liposome complex (QLC), which is the giant unilamellar vesicle with quantum dots (QDs) incorporated in its lipid bilayer. A spin coating method in conjunction with the electroformation technique yielded vesicles with highly homogeneous unilamellar structure. We observed QD size dependence of the QLC formation: QLCs form with blue, green and yellow-emission QD (core radius ~1.05 nm, 1.25 nm and 1.65 nm) but not with red-emission QD (core radius ~2.5 nm). In order to explain this size dependence, we made a simple model explaining the QD size effect on QLC formation in terms of the molecular packing parameter and the lipid conformational change. This model predicts that QDs below a certain critical size (radius ≈ 1.8 nm) can stably reside in a lipid bilayer of 4 - 5 nm in thickness for Egg-PC lipids. This is consistent with our previous experimental results. In the case of red-emission QD, QD-aggregations are only observed on the fluorescent microscopy instead of QLC. We expected that the reduction of packing parameter (P) would lead to the change of specific QD radius. This prediction could be verified by our experimental observation of the shift of the specific QD size by mixing DOPG.","PeriodicalId":59528,"journal":{"name":"生物物理学期刊(英文)","volume":"8 1","pages":"163-175"},"PeriodicalIF":0.0,"publicationDate":"2018-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43533377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-05-29DOI: 10.4236/OJBIPHY.2018.83010
D. Meijer, Hans J. H. Geesink
The current geometric and thermodynamic approaches in protein folding studies do not provide a definite solution to understanding mechanisms of folding of biological proteins. A major problem is that the protein is first synthesized as a linear molecule that subsequently must reach its native configuration in an extremely short time. Hydrophobicity-hydrophilicity models and random search mechanism cannot explain folding to the 3-D functional form in less than 1 second, as it occurs in the intact cell. We propose an integral approach, based on the embedding of proteins in the whole cellular context under the postulate: a life protein is never alone. In this concept the protein molecule is influenced by various long and short distance force fields of nature such as coherent electromagnetic waves and zero-point energy. In particular, the role of solitons is reviewed in relation to a novel GM-scale biophysical principle, revealed by us. This recent finding of a set of discrete EM frequency bands, that either promote or endanger life conditions, could be a key in further studies directed at the morphogenetic aspects of protein folding in a biological evolutionary context. In addition, an alternative hypothesis is presented in which each individual cell may store integral 3-D information holographically at the virtual border of a 4-D hypersphere that surrounds each living cell, providing a field receptive memory structure that is instrumental in guiding the folding process towards coherently oscillating protein networks that are crucial for cell survival.
{"title":"Guided Folding of Life's Proteins in Integrate Cells with Holographic Memory and GM-Biophysical Steering","authors":"D. Meijer, Hans J. H. Geesink","doi":"10.4236/OJBIPHY.2018.83010","DOIUrl":"https://doi.org/10.4236/OJBIPHY.2018.83010","url":null,"abstract":"The current geometric and thermodynamic approaches in protein folding studies do not provide a definite solution to understanding mechanisms of folding of biological proteins. A major problem is that the protein is first synthesized as a linear molecule that subsequently must reach its native configuration in an extremely short time. Hydrophobicity-hydrophilicity models and random search mechanism cannot explain folding to the 3-D functional form in less than 1 second, as it occurs in the intact cell. We propose an integral approach, based on the embedding of proteins in the whole cellular context under the postulate: a life protein is never alone. In this concept the protein molecule is influenced by various long and short distance force fields of nature such as coherent electromagnetic waves and zero-point energy. In particular, the role of solitons is reviewed in relation to a novel GM-scale biophysical principle, revealed by us. This recent finding of a set of discrete EM frequency bands, that either promote or endanger life conditions, could be a key in further studies directed at the morphogenetic aspects of protein folding in a biological evolutionary context. In addition, an alternative hypothesis is presented in which each individual cell may store integral 3-D information holographically at the virtual border of a 4-D hypersphere that surrounds each living cell, providing a field receptive memory structure that is instrumental in guiding the folding process towards coherently oscillating protein networks that are crucial for cell survival.","PeriodicalId":59528,"journal":{"name":"生物物理学期刊(英文)","volume":"8 1","pages":"117-154"},"PeriodicalIF":0.0,"publicationDate":"2018-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45649937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-05-29DOI: 10.4236/ojbiphy.2018.83011
J. Magnes, Cheris Congo, Miranda Hulsey-Vincent, H. Hastings, K. Raley-Susman
Using coherent light, we analyze the temporal diffraction at a single point from real-time living C. elegans locomotion in three-dimensional space. We describe the frequency spectrum of single swimming nematodes in an optical cuvette at a single sampling point in the far-field diffraction pattern. An analytical expression of the double slit is used to model the frequency spectra of nematodes as oscillating segments. The frequency spectrum in the diffraction pattern expands discretely and linearly as a multiple of the fundamental frequency with increasing distance from the central maximum. The frequency spectrum of a worm at a single point in the frequency spectrum contains all the frequencies involved in the locomotion and is used to characterize and compare nematodes. The occurrence of resonant frequencies in the dynamic diffraction pattern increases with the distance from the central maximum. The regular spacing of the resonant frequencies is used to identify characteristic swimming frequencies.
{"title":"Live C. elegans Diffraction at a Single Point","authors":"J. Magnes, Cheris Congo, Miranda Hulsey-Vincent, H. Hastings, K. Raley-Susman","doi":"10.4236/ojbiphy.2018.83011","DOIUrl":"https://doi.org/10.4236/ojbiphy.2018.83011","url":null,"abstract":"Using coherent light, we analyze the temporal diffraction at a single point from real-time living C. elegans locomotion in three-dimensional space. We describe the frequency spectrum of single swimming nematodes in an optical cuvette at a single sampling point in the far-field diffraction pattern. An analytical expression of the double slit is used to model the frequency spectra of nematodes as oscillating segments. The frequency spectrum in the diffraction pattern expands discretely and linearly as a multiple of the fundamental frequency with increasing distance from the central maximum. The frequency spectrum of a worm at a single point in the frequency spectrum contains all the frequencies involved in the locomotion and is used to characterize and compare nematodes. The occurrence of resonant frequencies in the dynamic diffraction pattern increases with the distance from the central maximum. The regular spacing of the resonant frequencies is used to identify characteristic swimming frequencies.","PeriodicalId":59528,"journal":{"name":"生物物理学期刊(英文)","volume":"08 1","pages":"155-162"},"PeriodicalIF":0.0,"publicationDate":"2018-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48776156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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