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Measurement of Metabolic and Inflammatory Serum Markers and Immune Marker Gene Expression during Superovulation in Beef Cattle 肉牛超数排卵过程中代谢和炎症血清标志物及免疫标志物基因表达的测定
Pub Date : 2021-04-01 DOI: 10.4236/OJAS.2021.112014
A. Snider, D. Mclean, A. Menino
Health status of donor cows during superovulation is important to ensure optimal embryo quality at time of collection. Because nutritional and metabolic status impact embryo quality some form of nutritional supplementation is often provided before and during superovulation. OmniGen-AF® (OG) feeding has been shown to assist in the maintenance of animal health through regulation of metabolic status and balance and supporting aspects of immune function. We observed feeding donor cows OG decreased percent degenerate embryos recovered following superovulation increased serum progesterone concentration and improved in vitro embryo development. Evaluation of OG feeding on markers of metabolic function and inflammatory and immune function in beef cattle embryo donors are reported here. Similarly, cow metabolic and inflammatory response with repeated superovulation protocols is not known. Biomarkers to monitor and evaluate cow health during superovulation may provide management options to improve embryo recovery and quality. Twenty-four Angus cross-bred cattle were randomly assigned to four treatment groups, fed 0 or 56 g/hd/day for 49 days and superovulated with 200 or 400 mg Folltropin V (FSH). Blood was collected weekly for analyses. The protocol was repeated on all cows 90 - 120 d later with cows reassigned to their original groups. No differences (P > 0.10) were observed due to OG feeding or FSH dose on metabolic and inflammatory markers. Replicate exerted a significant effect where serum concentration of albumin, IL1β, IL6, PGE2 and leptin were lower (P < 0.05) in Replicate 1 compared to 2. There was also a similar pattern of change in several of the metabolic and inflammatory markers during the superovulation protocol where concentrations were higher at the time of estrus and ovulation. Taken together, physiologic changes during the estrous cycle and the number of superovulation protocols can modulate metabolic markers and inflammatory response.
供体奶牛超排卵期的健康状况对保证胚胎采集时的最佳质量至关重要。由于营养和代谢状况会影响胚胎质量,因此在超排卵前和超排卵期间经常提供某种形式的营养补充。OmniGen-AF®(OG)喂养已被证明可以通过调节代谢状态和平衡以及支持免疫功能来帮助维持动物健康。我们观察到,饲喂OG可降低超排卵后退化胚胎的百分率,提高血清黄体酮浓度,促进体外胚胎发育。本文报道了OG饲喂对肉牛胚胎供体代谢功能、炎症和免疫功能标志物的影响。同样,母牛的代谢和炎症反应与重复的超排卵方案是未知的。在超排卵期间监测和评估奶牛健康的生物标志物可能为改善胚胎恢复和质量提供管理选择。选取24头安格斯杂交牛,随机分为4个处理组,分别饲喂0或56 g/hd/d,连续49 d,过排卵时分别添加200或400 mg促卵泡素V (FSH)。每周采集血液进行分析。90 ~ 120 d后,所有奶牛重复该方案,将奶牛重新分配到原组。OG饲喂或FSH剂量对代谢和炎症指标无显著差异(P > 0.10)。重复影响显著,重复1血清白蛋白、白细胞介素1β、白细胞介素6、PGE2和瘦素浓度低于重复2 (P < 0.05)。在超排卵过程中,几种代谢和炎症标志物也有类似的变化模式,在发情和排卵期浓度较高。综上所述,发情周期中的生理变化和超排卵方案的数量可以调节代谢标志物和炎症反应。
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引用次数: 0
Effect of Stabilized Fish Oil Source on Sperm Quality and Production of Boars 稳定鱼油来源对猪精子质量和产量的影响
Pub Date : 2021-04-01 DOI: 10.4236/OJAS.2021.112015
M. Parsley, M. Wilson, T. Gall, M. Ballard
Research findings for supplementing boar stud diets with fish oils are inconsistent. This study was designed to address three possible causes of performance variation of boars to fish oil supplementation: stability of the fatty acid source, level of inclusion and breed of boars tested. Three groups of 87 boars each, from two genetic lines (PIC 337 and PIC 800), were assigned to treatment based on age, mean sperm production (previous 12 weeks), and body condition score. All boars received a corn-soybean meal diet with a commercial fish oil supplement providing 1.83 g/boar/day of docosahexaenoic acid (DHA) as a preconditioning diet. On 10-Aug., 2020, the DHA source was changed to a stabilized starch imbedded source of refined fish oil (Salmate®), providing 1.83 g/b/d for the test diet. Two additional levels providing 2.38 and 2.94 g/b/d of DHA were fed for a 9 week pretreatment period and during the test period. Salmate® fed at 2.38 g/b/d of DHA resulted in a reduction in the number of rejected ejaculates (P 0.045) by 7.5% and 6.4% compared to the lowest and highest inclusion rates, respectively. There were no treatments by genetic line interactions. A retrospective study of semen production and quality of 77 boars on the Salmate® diet containing 1.83 g/b/d DHA was done to compare to the original source of DHA at the same inclusion level. There were no differences in semen quality parameters between the 2 lipid sources. Ejaculate volume increased from 177.9 ml to 233.4 ml (P 0.001) and total sperm cells per ejaculate increased from 69.7 × 109 to 82.0 × 109 (P 0.001) due to substitution of Salmate®. Adding Salmate® at 2.38 g/b/d resulted in a lower number of rejected ejaculates per boar by 7.5% and 6.4% vs. 1.83 and 2.94 g/b/d, respectively, and boars fed Salmate® at 1.83 g/b/d produced 17% more doses than the competing product.
关于在种公猪日粮中添加鱼油的研究结果并不一致。本研究旨在解决3个可能导致公猪对鱼油添加性能变化的原因:脂肪酸来源的稳定性、添加水平和被试公猪的品种。来自两个遗传系(PIC 337和PIC 800)的3组公猪,每组87头,根据年龄、平均精子产量(前12周)和身体状况评分进行处理。所有公猪均饲喂玉米-豆粕型饲粮,并添加1.83 g/头猪/天二十二碳六烯酸(DHA)的商业鱼油作为预处理饲粮。10-Aug。到2020年,将DHA来源改为稳定淀粉包埋的精制鱼油(Salmate®)来源,为试验日粮提供1.83 g/b/d。在9周的预处理期和试验期内,分别饲喂DHA含量为2.38和2.94 g/b/d的另外两个水平。在盐酸盐中添加2.38 g/b/d的DHA,与添加率最低和最高的盐酸盐相比,拒绝射精的数量分别减少了7.5%和6.4% (P 0.045)。没有遗传系互作处理。本研究对77头公猪在添加1.83 g/b/d DHA的盐盐®饲粮中精液产量和质量进行了回顾性研究,并与原饲粮中DHA添加水平相同的情况下进行了比较。两种脂质来源的精液质量参数无差异。由于Salmate®的替代,射精量从177.9 ml增加到233.4 ml (P 0.001),每次射精的精子细胞总数从69.7 × 109增加到82.0 × 109 (P 0.001)。与1.83 g/b/d和2.94 g/b/d相比,添加2.38 g/b/d的盐酸盐®可使每头公猪的拒绝射精量分别降低7.5%和6.4%,而饲喂1.83 g/b/d的盐酸盐®的公猪的拒绝射精量比同类产品多17%。
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引用次数: 1
Evaluation of Models for Longevity in a Closed Nucleus Breeding Programme: A Case Study Kenyan Sahiwal 闭核育种项目中寿命模型的评估:肯尼亚Sahiwal的案例研究
Pub Date : 2021-04-01 DOI: 10.4236/OJAS.2021.112021
Benjamin Musyimi Musingi, Leah Mumbi Mahianyu, E. D. M. Musingi
Longevity and stay ability are parameters in any breeding programme. The Overstaying of any animal on a farm has got negative effects on the farmer. Short stay also means early exit of good animals that could have been used for a longer time and more productive. The intention of this review paper is to emphasize the importance of models that an upcoming breeding program can use without affecting the quality of the herd. In this paper, longevity is taken purely on the performance measure. The functional and type traits of longevity should be studied further before all models are put into play. But the case of closed breeding programmes, performance traits are easily measurable because many animals will exit the herd as they have been sold to farmers hence many functional straits will be expressed outside the herd. Genetic evaluation of the Kenyan Sahiwal should account for inbreeding. This study has also provided genetic and phenotypic parameters to enable the inclusion of longevity in the breeding objective for the Sahiwal cattle improvement programme.
寿命和停留能力是任何育种计划的参数。农场里任何动物的过度饲养都会对农民产生负面影响。短期停留也意味着可以使用更长时间、更高产的好动物提前离开。这篇综述论文的目的是强调即将到来的育种计划可以在不影响牛群质量的情况下使用的模型的重要性。在本文中,寿命纯粹是根据性能衡量的。在所有模型投入使用之前,应进一步研究寿命的功能和类型特征。但在封闭式繁殖计划的情况下,性能特征很容易测量,因为许多动物在出售给农民后会退出畜群,因此许多功能性困境将在畜群之外表现出来。肯尼亚Sahiwal的基因评估应该考虑近亲繁殖。这项研究还提供了遗传和表型参数,以便将寿命纳入Sahiwal牛改良计划的育种目标。
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引用次数: 0
Effects of Feeding OmniGen-AF® during Superovulation on in Vitro Development of Embryos Recovered from Donor Beef Cows 超排卵期饲喂OmniGen-AF®对供体肉牛体外再生胚胎发育的影响
Pub Date : 2021-01-26 DOI: 10.4236/OJAS.2021.111004
A. Snider, D. Mclean, A. Menino
Embryo quality is crucial when selecting embryos for transfer. Variation in quality may be attributed to poor oocytes, semen, stress, inflammation, and potential immune system dysregulation. OmniGen-AF® (OG) feeding supports immune system function and animal health. Our laboratory recently reported lower percent degenerate embryos recovered and increased plasma progesterone in beef cattle donors fed OG during superovulation. In vitro development of embryos recovered from donor cows fed OG prior to collection is presented here. Embryos were recovered from 24 beef cows assigned to four treatment groups: 0 g OG/hd/d and 200 mg Folltropinr 0 g OG/hd/d and 400 mg FSH (0/400), 56 g OG/hd/d, 200 mg FSH (56/200) and 56 g OG/hd/d and 400 mg FSH (56/400). Good to excellent quality early blastocysts were cultured for 8 d. and development through hatching, embryonic volume and plasminogen activator (PA) production were quantified. The complete protocol was repeated 90 - 120 d later as Replicate 2. Optimal development was observed by embryos recovered from 0/200 cows where percent blastocysts hatching was greater (P compared to 56/200 and 0/400 cows and embryonic volume was greatest (P was similar (P > 0.10) to 56/400 cows and embryos recovered from 56/400 cows in Replicate 1 produced more (P in vitro embryo development similar to that observed for 0/200 cows.
在选择胚胎进行移植时,胚胎质量至关重要。质量的变化可能归因于卵母细胞、精液、压力、炎症和潜在的免疫系统失调。OmniGen-AF®(OG)饲料支持免疫系统功能和动物健康。我们的实验室最近报道了在超排卵期间饲喂OG的肉牛供体的退化胚胎恢复率较低,血浆黄体酮增加。在收集之前,从饲喂OG的供体奶牛中恢复的胚胎的体外发育在这里提出。选取24头肉牛,将胚胎分为4个处理组:0 g OG/hd/d + 200 mg Folltropinr 0 g OG/hd/d + 400 mg FSH(0/400)、56 g OG/hd/d、200 mg FSH(56/200)和56 g OG/hd/d + 400 mg FSH(56/400)。将优质至优良的早期囊胚培养8 d,通过孵化发育,定量测定胚胎体积和纤溶酶原激活物(PA)的产量。90 - 120d后重复完整的方案作为复制2。0/200奶牛回收的胚胎发育最佳,囊胚孵化率高于56/200和0/400奶牛(P < 0.05),胚胎体积最大(P < 0.05),与56/400奶牛相似,重复1中56/400奶牛回收的胚胎体外胚胎发育率与0/200奶牛相似。
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引用次数: 0
Effects of Guar (Cyamopsis tetragonoloba) Residues on the Performance and Nutrients Digestibility in Finishing Awassi Lambs 瓜尔豆(Cyamopsis tetragonoloba)残留对育成Awassi羔羊生产性能和养分消化率的影响
Pub Date : 2021-01-26 DOI: 10.4236/OJAS.2021.111008
J. A. Omar, A. Zaazaa, Maen N. Sheqwarah, B. A. Shanab, W. Qaisi, J. Abdallah
The nutritive quality of guar (Cyamopsis tetragonoloba) residues (GR) was investigated and the effects of partial replacement of wheat straw by GR on nutrients intake, digestibility, and growth performance. Twenty four male lambs, with average body weight (BW) of 40.3 ± 2.5 kg were divided into three groups. Lambs were fed individually for 104 days with concentrate mixture (18% crude protein) and wheat straw as a total mixed ration. Wheat straw was replaced with 0, 100 and 145 g/kg dry matter guar residues. All rations were isonitrogenous and isocaloric. The fattening trial lasted 104 days. At the end of week 10 of the trail, a digestion trial was performed using six lambs from each group. The composition of nutrients in the GR was 87%, 8.5%, 56.4% and 40.1% for DM, CP, NDF and ADF, respectively. DM and crude protein (CP) intake were not affected by the inclusion of GR. However, neutral detergent fibre (NDF) and acid detergent fibre (ADF) intake were lower in lambs fed GR compared to control lambs. At the end of the experiment, lambs fed 100 and 145 g GR/kg DM diets gained more weight (P version (CR). Results from this work suggested that GR had advantages compared to regular roughage (wheat straw) in regard to parameters investigated as well as the significant reduction in fattening diets cost.
研究了瓜尔豆(Cyamopsis tetragonoloba)残渣(GR)的营养品质,以及GR部分替代麦秸对营养物质摄入、消化率和生长性能的影响。选取24只平均体重(BW)为40.3±2.5 kg的公羊羔分为3组。以粗蛋白质含量为18%的精料混合日粮与麦秸混合饲喂,试验期104 d。用0、100和145 g/kg干物质瓜尔瓜尔渣代替麦秸。所有的口粮都是等氮和等热量的。育肥试验期104 d。在试验第10周末,每组取6只羔羊进行消化试验。GR中DM、CP、NDF和ADF的营养成分组成分别为87%、8.5%、56.4%和40.1%。饲粮中添加GR对DM和粗蛋白质(CP)采食量没有影响,但中性洗涤纤维(NDF)和酸性洗涤纤维(ADF)采食量低于对照组。试验结束时,饲粮中添加100和145 g GR/kg DM的羔羊体重(P版)增加较多。本研究结果表明,与普通粗饲料(麦秸)相比,GR在研究参数方面具有优势,并且显著降低了育肥饲料的成本。
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引用次数: 2
Carcass and Meat Characteristics of Bulls from Arsi, Boran, Harar and Holstein Frisian Crosses Cattle Breeds Finished under Similar Level of Concentrate Supplementation 阿西、博兰、哈拉尔和荷斯坦-弗里斯兰杂交品种公牛在相同精料添加水平下的胴体和肉质特性
Pub Date : 2021-01-26 DOI: 10.4236/OJAS.2021.111002
A. Musa, Y. Mummed, Mohammed Y. Kurtu, M. Temesgen, T. O’Quinn
A study was conducted to evaluate the effect of age and breeds (Arsi, Borana, HF-Cross and Harar) on carcass and meat characteristics of bulls finished under similar feeding conditions. The feeding experiment was conducted for 90 days at Beef farm of Haramaya University. In this experiment, 24 bulls were fed with roughage (60%) which contained grass hay and wheat straw and concentrate (40%) which contained wheat bran, noug (gucia abysica) cake, maize grain, limestone, salt and ruminant premix. The bulls were transported to Bishoftu ELFORA export abattoir for slaughter following the procedure of the abattoir. The result of the study revealed that the average slaughter weight, hot carcass weight, cold carcass weight, shrink loss, commercial dressing and true dressing percentage were 179.1 kg, 86.8 kg, 82.7 kg 4.7%, 48.8% and 78.3%, respectively. Total edible and nonedible offal were significantly (P significantly (P Boran, Harar, and HF-crossbred were 78.1%, 77%, 72.8% and 77.2% respectively. Meat yield was predicted from fat thickness and ribeye area with 61% accuracy. Arsi bulls attained the highest (78%) meat yield at early age but decreased by 0.713 rate as age of animals advanced by one digit whereas the meat yield from other breeds showed increment in meat yield percentage with the rate of 1.98, 1.1 and 0.1 for cross, Borana and Harar breed bulls, respectively as age advanced. Yield from fore shank, sirloin, top and bottom sirloin primal cuts were affected by breed. Arsi breed had higher meat bone ration than cross breed. Retailed meat yield was significantly (P 0.05) by age and breed in the current study.
研究了年龄和品种(Arsi、Borana、HF Cross和Harar)对相似饲养条件下产公牛胴体和肉质特性的影响。饲养试验在Haramaya大学牛肉农场进行,为期90天。在本实验中,24头公牛被喂以粗饲料(60%)和精饲料(40%),粗饲料中含有草干草和麦秆,精饲料中含有麦麸、牛(gucia abilica)饼、玉米粒、石灰石、盐和反刍动物预混料。公牛被运往Bishoftu ELFORA出口屠宰场,按照屠宰场的程序进行屠宰。研究结果表明,平均屠宰重量为179.1kg,热胴体重量为86.8kg,冷胴体重量为82.7kg,收缩率为48.8%,商品化率为78.3%。食用内脏和非食用内脏总量显著(P显著(P分别为78.1%、77%、72.8%和77.2%)。根据脂肪厚度和肋叶面积预测肉产量的准确率为61%。阿尔西公牛早期的肉产量最高(78%),但随着动物年龄的增长,产量下降了0.713%,而其他品种的肉产量杂交公牛、博拉纳公牛和哈拉尔公牛的产肉率随年龄的增长分别为1.98、1.1和0.1。前腰、里脊肉、上下里脊肉原始切口的产量受品种影响。阿尔西种肉骨比杂交种高。在本研究中,不同年龄和品种的零售肉产量显著(P 0.05)。
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引用次数: 5
Fishing and Rural Livelihood: A Philippine Context 渔业和农村生计:菲律宾的背景
Pub Date : 2021-01-26 DOI: 10.4236/OJAS.2021.111007
Rosalina Palanca-Tan, Sheila Bongat-Bayog
The Municipality of Lake Sebu in Mindanao, Philippines offers various opportunities for income-generating fishing activities with its abundant surface water bodies. Lake Sebu is particularly known for good quality tilapia, farmed in fish cages. This study employed primary data collection methods, namely key informant interview, focus group discussion and a comprehensive livelihood household survey to assess the conditions and problems constraining fishing households in Lake Sebu. The study found that fishing-related benefits were mainly derived from aquaculture. Capture fisheries are essentially very small-scale, low gear open fishing done by local fisher folk that generates only subsistence income. Local residents are mostly engaged in retail fish trading that likewise generate only subsistence income. Survey results also revealed large variations in the scale of aquaculture operations. Fish cage owners, who are not originally from Lake Sebu or have residences outside Lake Sebu, are usually the large-scale fish farm operators, while the locals are only engaged in small-scale aquaculture due to limited financial sources. The study found that on the average, fishing-dependent households earned an annual income of PHP 132,800 (US$ 2619), which was 15% lower than the average for all household respondents, and that a substantial 63% of fishing-dependent households live below the poverty line. It appears, therefore, that the rich water resources in the municipality have not contributed substantially to poverty alleviation. These findings point to the need for government assistance such as aquaculture financing programs and the formation of fish farming cooperatives to enable locals to engage in large-scale fish farming and reap the benefits of economies of scale.
菲律宾棉兰老岛塞布湖市拥有丰富的地表水体,为创收捕鱼活动提供了各种机会。塞布湖以网箱养殖的优质罗非鱼而闻名。本研究采用主要数据收集方法,即关键信息人访谈、焦点小组讨论和全面的生计家庭调查,来评估塞布湖捕鱼家庭的条件和问题。研究发现,与渔业相关的利益主要来自水产养殖。捕捞渔业基本上是由当地渔民进行的非常小规模、低渔具的公开捕鱼,只产生维持生计的收入。当地居民大多从事鱼类零售贸易,同样只能赚取维持生计的收入。调查结果还显示,水产养殖的规模差异很大。鱼笼主人不是塞布湖人,也不是在塞布湖外有住所,通常是大型养鱼场的经营者,而当地人由于资金来源有限,只从事小型水产养殖。该研究发现,依赖捕鱼的家庭平均年收入为132800菲律宾比索(2619美元),比所有家庭受访者的平均水平低15%,相当一部分63%的家庭生活在贫困线以下。因此,该市丰富的水资源似乎没有对减贫作出实质性贡献。这些发现表明,需要政府援助,如水产养殖融资计划和成立养鱼合作社,使当地人能够从事大规模养鱼,并从规模经济中获益。
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引用次数: 1
Factors Associated with Increased Walleye Production in Lined Compared to Earthen-Substrate Ponds 与泥土基质池相比,衬砌池中Walleye产量增加的相关因素
Pub Date : 2021-01-26 DOI: 10.4236/OJAS.2021.111005
M. Ward, Brian G. Blackwell
Walleye (Sander vitreus) fingerling production can be enhanced when the pond substrate is altered from earthen soils to an exposed liner; however, few differences in water chemistry, prey densities, and food habits have been identified that could potentially explain this production difference. Lack of an explanation led to further comparison of walleye fingerling culture between substrate types during 2016. Two ponds of each type were stocked with 1- to 3-day-old walleye fry, but lined ponds received an additional 104,166 fry/ha and were stocked 1 or 2 days later than earthen ponds (May 10). Walleyes in lined ponds achieved a growth advantage on May 31 (+2.5 mm) and on June 6 (+3.3 mm), but lengths were not significantly different at harvest (June 15). Lined ponds increased walleye yield and number 1.8-fold while not reducing individual fish size at harvest compared to earthen ponds. Walleye food habits were generally similar between pond types with a greater number of zooplankton being consumed through June 6 followed by an increase in larval chironomid consumption at harvest (June 15). After May 30, lined ponds exhibited lower dissolved oxygen and higher ammonia-nitrogen concentrations even though earthen ponds received additional (157 kg/ha) alfalfa (Medicago sativa) meal fertilizer. Also, at this time pH declined abruptly and remained below 8.2 in lined ponds. Moreover, the enhanced production in lined ponds coincided with walleyes that exhibited an increased pelagic (δ13C) signature during the culture period (2‰ to 3.3‰ more negative). Efforts to improve production in earthen-substrate ponds should focus on increasing the pelagic signature of the food web through fertilization strategies which increase ammonia-nitrogen (≈0.3 mg/L) and decrease dissolved oxygen (≈6.5 mg/L) while maintaining a pH between 8.0 and 8.2 during the second half of the rearing interval.
当池塘基质从泥土变为裸露的衬垫时,Walleye(Sander vitreus)鱼种的产量可以提高;然而,在水化学、猎物密度和食物习惯方面几乎没有发现可以解释这种生产差异的差异。由于缺乏解释,2016年期间对不同基质类型的walleye鱼种培养进行了进一步比较。每种类型的两个池塘都放养了1到3天大的walleye鱼苗,但有内衬的池塘每公顷额外放养104166只鱼苗,比土池塘晚了1到2天(5月10日)。在5月31日(+2.5毫米)和6月6日(+3.3毫米),有内衬的池塘中的Walleyes获得了生长优势,但在收获时(6月15日),长度没有显著差异。与土制池塘相比,有内衬的池塘使大眼鱼的产量和数量增加了1.8倍,但在收获时并没有减少个体鱼的大小。Walleye的食物习惯在不同的池塘类型之间通常是相似的,直到6月6日,消耗了更多的浮游动物,随后在收获时(6月15日),幼虫的摇蚊消耗量增加。5月30日之后,尽管土制池塘增加了157公斤/公顷的苜蓿(Medicago sativa)粉肥,但有内衬的池塘表现出较低的溶解氧和较高的氨氮浓度。此外,此时pH值突然下降,并在有内衬的池塘中保持在8.2以下。此外,在有内衬的池塘中,产量的提高与walleyes相吻合,walleyes在培养期间表现出增加的远洋(δ13C)特征(负向增加2‰至3.3‰)。提高土底塘产量的努力应侧重于通过施肥策略增加食物网的上层特征,施肥策略增加氨氮(≈0.3 mg/L)和溶解氧(≈6.5 mg/L),同时在饲养间隔的后半段将pH值保持在8.0至8.2之间。
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引用次数: 2
Comparative Growth Performance, Carcass Characteristics and Meat Quality of Local Horro and Exotic Cockerels of Tropical Origin Fed Growers Diet 热带原产本地和外来公鸡饲养日粮的生长性能、胴体特征和肉质比较
Pub Date : 2021-01-26 DOI: 10.4236/OJAS.2021.111006
Abrar Biazen, U. Mengistu, A. Negassi, Aseffa Getenet, A. Solomon, D. Tadelle
Growth performance, carcass characteristics and meat quality of male chickens were evaluated using eight-week-old 360 cockerels from three exotic (Koekoek, Kuroiler and Sasso-R) and one Ethiopian indigenous (Horro) genotypes. Cockerels in each breed (90) were randomly allocated into three replications of 30 birds each and housed in deep litter pens for the experiment that lasted for 8 to 16 weeks. The experiment was arranged in a Completely Randomized Design. Seven birds per breed were slaughtered at the end to determine carcass yield, chemical composition and quality attributes of meat. The Kuroiler breed has higher body weight, body weight change (1621.8 g), average daily gain (28.9 g/day), daily feed consumption (127.4 g/day) and better feed conversion ratio (4.40) than other breeds. The Kuroiler breed has heavier slaughter weight (2716.3 g), dressed carcass weight (2201.0 g), eviscerated carcass weight (1905.1 g), breast weight (537.7 g), thigh weight (303.1 g) and drumstick weight (274.8 g) than other breeds. Total edible offal was higher for Kuroiler (324.2 g) and Sasso-R (306.1 g), intermediate in Koekoek (204.4 g) and low in Horro (152.4 g) breed. The relative proportion of breast and drumstick (% slaughter weight) were lower in Koekoek and Sasso-R (17.3 and 8.9) breeds, respectively. Abdominal fat weight (13.7 g and 1.02%) was lowest in Horro. Sasso-R showed higher percent edible offal weight (13.4%) than Horro breed (11.4%). The DM content in breast and thigh meat was higher for Horro breed than the rest. The CP content in the breast and thigh meat was lower for the Kuroiler and Koekoek breeds, respectively. Higher EE in the thigh meat was recorded for Sasso-R (8.9%), whereas, EE content in the breast meat was similar (3.4% - 3.7%) among the breeds. Flavor, tenderness, juiciness and general acceptability of breast and thigh meat were not affected by breed. The highest net return was observed in the Kuroiler breed. Based on the results obtained, it is suggested that the Kuroiler breed can be used for cockerel meat production in Ethiopia where intensive broiler production is not well developed.
选用3种外来基因型(Koekoek、Kuroiler和Sasso-R)和1种埃塞俄比亚本土基因型(Horro)的8周龄360公鸡,对公鸡的生长性能、胴体特性和肉质进行了评价。每个品种的小公鸡(90只)随机分为3个重复,每个重复30只,饲养在深窝中,持续8 ~ 16周。试验采用完全随机设计。每个品种末屠宰7只,测定胴体产量、肉的化学成分和品质指标。库罗尔品种的体重、体重变化(1621.8 g)、平均日增重(28.9 g/d)、日采食量(127.4 g/d)和饲料系数(4.40)均高于其他品种。Kuroiler品种的屠宰重(2716.3 g),屠宰胴体重(2201.0 g),全膛胴体重(1905.1 g),胸重(537.7 g),大腿重(303.1 g)和鸡腿重(274.8 g)比其他品种更重。可食用内脏总量库鲁鸡(324.2 g)和沙索- r (306.1 g)较高,库鲁鸡居中(204.4 g),霍罗鸡较低(152.4 g)。Koekoek和Sasso-R品种的胸肉和鸡腿的相对比例(屠宰重的百分比)较低(分别为17.3和8.9)。腹部脂肪质量最低,为13.7 g,为1.02%。沙索- r品种可食用内脏重量的百分比(13.4%)高于霍罗品种(11.4%)。Horro品种的胸肉和大腿肉中DM含量高于其他品种。库鲁犬和库奇犬的胸肉和大腿肉中粗蛋白质含量较低。沙索- r鸡腿肉的粗脂肪含量较高(8.9%),胸肉的粗脂肪含量基本相同(3.4% ~ 3.7%)。鸡胸肉和大腿肉的风味、嫩度、多汁性和一般可接受性不受品种的影响。净收益最高的品种是库罗尔犬。根据所得结果,建议在集约化肉鸡生产不发达的埃塞俄比亚,Kuroiler品种可用于公鸡肉生产。
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引用次数: 8
Sex-Based Variation of Gene Expression in the Gonads and Fins of Russian Sturgeon (Acipenser gueldenstaedtii) 俄罗斯鲟性腺和鳍基因表达的性别变异
Pub Date : 2021-01-26 DOI: 10.4236/OJAS.2021.111001
G. Degani, Akram Hajouj, A. Hurvitz
Russian sturgeon (Acipenser gueldenstaedtii) is a primitive freshwater fish and a source of black caviar. The genes involved in sexual determination and differentiation are still unknown and there are no molecular markers for sex identification in this species. Studying the variation of the sex-based differences in genomic sequences and in gene expression in the sturgeon may lead to markers of sex in early stages of development and advances in aquaculture, as well as provide novel insights about the evolution of reproduction, sex determination, and sexual differentiation mechanisms in vertebrates. Previous studies by our and other groups have identified differentially expressed genes in the gonads of adult female and male sturgeon. The current study aimed to test whether these genes were also differentially expressed in non-gonadal tissue, namely fins. We measured by qRT-PCR the mRNA levels of 29 known and novel sex-related genes in the gonads and fins of males (4 years old) and females (7 years old; sexual maturation is earlier in males than in females). Six genes (ATP6, IGFRM, LIA1A, S1A, NPL1A, GAPDH and SOX9) showed higher expression in female fins. However, only ATP6 mRNA levels differed in fins of males and females of the same age (4 years old). These findings underscore the impracticality of sex identification based on gene expression in non-gonadal tissue and the need for genetic sex markers in the Russian sturgeon.
俄罗斯鲟是一种原始淡水鱼,也是黑鱼子酱的来源。参与性别决定和分化的基因仍然未知,并且在该物种中没有用于性别鉴定的分子标记。研究鲟鱼基因组序列和基因表达中基于性别的差异的变化,可能会在水产养殖的发展和进步的早期阶段发现性别标记,并为脊椎动物繁殖、性别决定和性别分化机制的进化提供新的见解。我们和其他研究小组先前的研究已经确定了成年雌性和雄性鲟鱼性腺中差异表达的基因。目前的研究旨在测试这些基因是否在非性腺组织,即鳍中也有差异表达。我们通过qRT-PCR测量了雄性(4岁)和雌性(7岁;雄性的性成熟早于雌性)性腺和鳍中29个已知和新的性相关基因的mRNA水平。6个基因(ATP6、IGFRM、LIA1A、S1A、NPL1A、GAPDH和SOX9)在雌性鳍中表现出较高的表达。然而,在相同年龄(4岁)的雄性和雌性的鳍中,只有ATP6 mRNA水平不同。这些发现强调了基于非性腺组织中基因表达的性别鉴定的不切实际,以及对俄罗斯鲟遗传性别标记的需要。
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引用次数: 3
期刊
动物科学期刊(英文)
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