Journal of Materials Science: Materials in Medicine最新文献_第3页

Assessment of cranial reconstruction utilizing various implant materials: finite element study 利用各种植入材料进行颅骨重建的评估：有限元研究。

IF 4.2 3区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of Materials Science: Materials in Medicine

Pub Date : 2024-08-13 DOI: 10.1007/s10856-024-06816-9

Yomna H. Shash

The human head can sometimes experience impact loads that result in skull fractures or other injuries, leading to the need for a craniectomy. Cranioplasty is a procedure that involves replacing the removed portion with either autologous bone or alloplastic material. While titanium has traditionally been the preferred material for cranial implants due to its excellent properties and biocompatibility, its limitations have prompted the search for alternative materials. This research aimed to explore alternative materials to titanium for cranial implants in order to address the limitations of titanium implants and improve the performance of the cranioplasty process. A 3D model of a defective skull was reconstructed with a cranial implant, and the implant was simulated using various stiff and soft materials (such as alumina, zirconia, hydroxyapatite, zirconia-reinforced PMMA, and PMMA) as alternatives to titanium under 2000N impact forces. Alumina and zirconia implants were found to reduce stresses and strains on the skull and brain compared to titanium implants. However, PMMA implants showed potential for causing skull damage under current loading conditions. Additionally, PMMA and hydroxyapatite implants were prone to fracture. Despite these findings, none of the implants exceeded the limits for tensile and compressive stresses and strains on the brain. Zirconia-reinforced PMMA implants were also shown to reduce stresses and strains on the skull and brain compared to PMMA implants. Alumina and zirconia show promise as alternatives to titanium for the production of cranial implants. The use of alternative implant materials to titanium has the potential to enhance the success of cranial reconstruction by overcoming the limitations associated with titanium implants.

Graphical Abstract

人的头部有时会受到冲击负荷，导致颅骨骨折或其他损伤，从而需要进行颅骨切除术。颅骨成形术是一种用自体骨或异体材料替代切除部分的手术。虽然钛因其优异的性能和生物相容性一直是颅骨植入物的首选材料，但其局限性促使人们寻找替代材料。本研究旨在探索用于颅骨植入物的钛替代材料，以解决钛植入物的局限性，提高颅骨成形术的性能。研究人员用颅骨植入体重建了一个有缺陷的颅骨三维模型，并使用各种软硬材料（如氧化铝、氧化锆、羟基磷灰石、氧化锆增强聚甲基丙烯酸甲酯和聚甲基丙烯酸甲酯）模拟了植入体在 2000N 冲击力下替代钛的情况。与钛植入物相比，氧化铝和氧化锆植入物可减少头骨和大脑的应力和应变。不过，PMMA 植入物显示在当前加载条件下有可能造成颅骨损伤。此外，PMMA 和羟基磷灰石植入物容易发生断裂。尽管有这些发现，但没有一个植入物超过大脑的拉伸和压缩应力和应变极限。与 PMMA 植入体相比，氧化锆增强 PMMA 植入体还能减少头骨和大脑的应力和应变。氧化铝和氧化锆有望成为钛的替代品，用于生产颅骨植入物。使用钛的替代植入体材料有可能克服钛植入体的局限性，从而提高颅骨重建的成功率。

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引用次数: 0

Feasibility of using diamond-like carbon films in total joint replacements: a review 在全关节置换术中使用类金刚石碳膜的可行性：综述。

IF 4.2 3区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of Materials Science: Materials in Medicine

Pub Date : 2024-08-13 DOI: 10.1007/s10856-024-06814-x

Anurag Roy, Annette Bennett, Lisa Pruitt

Diamond-like Carbon (DLC) has been used as a coating material of choice for a variety of technological applications owing to its favorable bio-tribo-thermo-mechanical characteristics. Here, the possibility of bringing DLC into orthopedic joint implants is examined. With ever increasing number of patients suffering from osteoarthritis as well as with the ingress of the osteoarthritic joints’ malaise into younger and more active demographics, there is a pressing need to augment the performance and integrity of conventional total joint replacements (TJRs). Contemporary joint replacement devices use metal-on-polymer articulations to restore function to worn, damaged or diseased cartilage. The wear of polymeric components has been addressed using crosslinking and antioxidants; however, in the context of the metallic components, complications pertaining to corrosion and metal ion release inside the body still persist. Through this review article, we explore the use of DLC coatings on metallic bearing surfaces and elucidate why this technology might be a viable solution for ongoing electrochemical challenges in orthopedics. The different characteristics of DLC coatings and their feasibility in TJRs are examined through assessment of tribo-material characterization methods. A holistic characterization of the coating-substrate interface and the wear performance of such systems are discussed. As with all biomaterials used in TJRs, we need mindful consideration of potential in-vivo challenges. We present a few caveats for DLC coatings including delamination, hydrophobicity, and other conflicting as well as outdating findings in the literature. We recommend prudently exploring DLC films as potential coatings on metallic TJR components to solve the problems pertaining to wear, metal ion release, and corrosion. Ultimately, we advise bringing DLC into clinical use only after addressing all challenges and concerns outlined in this article.

Graphical Abstract

类金刚石碳（DLC）具有良好的生物三热力学特性，已被用作各种技术应用的首选涂层材料。本文将探讨将 DLC 应用于骨科关节植入物的可能性。随着骨关节炎患者人数的不断增加，以及骨关节炎患者逐渐年轻化和活跃化，迫切需要提高传统全关节置换术（TJR）的性能和完整性。当代关节置换装置使用金属聚合物关节来恢复磨损、受损或病变软骨的功能。聚合物组件的磨损问题已通过交联和抗氧化剂得到解决；但在金属组件方面，与腐蚀和体内金属离子释放有关的并发症仍然存在。通过这篇综述文章，我们探讨了在金属轴承表面使用 DLC 涂层的问题，并阐明了为什么这项技术可能成为解决整形外科电化学难题的可行方案。通过对三材料表征方法的评估，研究了 DLC 涂层的不同特性及其在 TJR 中的可行性。还讨论了涂层-基底界面的整体表征以及此类系统的磨损性能。与所有用于 TJR 的生物材料一样，我们需要认真考虑潜在的体内挑战。我们提出了 DLC 涂层的一些注意事项，包括分层、疏水性以及文献中其他相互矛盾和过时的研究结果。我们建议审慎探索将 DLC 薄膜作为 TJR 金属组件的潜在涂层，以解决磨损、金属离子释放和腐蚀等相关问题。最后，我们建议只有在解决了本文概述的所有挑战和问题后，才能将 DLC 用于临床。

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引用次数: 0

Improvement photothermal property of MoS2/Fe3O4/GNR nanocomposite in cancer treatment 改善 MoS2/Fe3O4/GNR 纳米复合材料在癌症治疗中的光热特性。

IF 4.2 3区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of Materials Science: Materials in Medicine

Pub Date : 2024-08-13 DOI: 10.1007/s10856-024-06819-6

Behdad Shariati, Mohammad Taghi Goodarzi, Alireza Jalali, Nasrin Salehi, Majid Mozaffari

The objective of the present study was to develop a novel molybdenum disulfide/iron oxide/gold nanorods (MoS₂/Fe₃O₄/GNR) nanocomposite (MFG) with different concentrations of AgNO₃ solution (MFG1, MFG2, and MFG3) for topical doxorubicin (DOX) drug delivery. Then, these nanocomposites were synthesized and characterized by Fourier transform infrared (FTIR), Transmission electron microscopy (TEM), Dynamic light scattering (DLS), and Ultraviolet-visible (UV–Vis) spectroscopies to confirm their structural and optical properties. Cytotoxicity of samples on Hela cell was determined using MTT assay. Results indicated that nanocomposites possess little cytotoxicity without NIR laser irradiation. Also, the relative viabilities of Hela cells decreased when the concentration of AgNO₃ solution increased in this nanocomposite. Using NIR irradiation, the relative viabilities of Hela cells decreased when the concentration of samples increased. Acridine orange/propidium iodide (PI) staining, flow cytometry were recruited to evaluate the effect of these nanocomposites on apoptosis of Hela cells. Finally, results revealed when DOX loading increased in nanocomposite, then cell viability was decreased in it. Therefore, these properties make MFG3 nanocomposite a good candidate for photothermal therapy and drug loading.

Graphical Abstract

本研究旨在开发一种新型二硫化钼/氧化铁/金纳米棒（MoS2/Fe3O4/GNR）纳米复合材料（MFG），并将其与不同浓度的 AgNO3 溶液（MFG1、MFG2 和 MFG3）混合，用于局部给药多柔比星（DOX）。然后，合成了这些纳米复合材料，并通过傅立叶变换红外光谱（FTIR）、透射电子显微镜（TEM）、动态光散射（DLS）和紫外可见光谱（UV-Vis）对其进行表征，以确认其结构和光学特性。使用 MTT 试验测定了样品对 Hela 细胞的细胞毒性。结果表明，在没有近红外激光照射的情况下，纳米复合材料的细胞毒性很小。此外，当该纳米复合材料中的 AgNO3 溶液浓度增加时，Hela 细胞的相对活力降低。使用近红外激光照射时，当样品浓度增加时，Hela 细胞的相对存活率降低。利用吖啶橙/碘化丙啶（PI）染色法和流式细胞术评估了这些纳米复合材料对 Hela 细胞凋亡的影响。结果表明，当纳米复合材料中的 DOX 负荷增加时，细胞活力降低。因此，这些特性使 MFG3 纳米复合材料成为光热疗法和药物负载的良好候选材料。

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引用次数: 0

Prolonged retention of luliconazole nanofibers for topical mycotic condition: development, in vitro characterization and antifungal activity against Candida albicans 用于局部真菌病的氟环唑纳米纤维的长期保留：开发、体外表征和对白色念珠菌的抗真菌活性。

IF 4.2 3区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of Materials Science: Materials in Medicine

Pub Date : 2024-08-08 DOI: 10.1007/s10856-024-06815-w

Akashkumar Doshi, Bala Prabhakar, Sarika Wairkar

An antifungal agent, luliconazole, is commercially available in cream or gel form. The major limitation of these conventional formulations is less residence time at the infection site. The primary objective of this work was to develop luliconazole-loaded polyvinyl alcohol (Luz-PVA) nanofibers for mycotic skin conditions with a longer retention. Luz-PVA nanofibers were prepared by plate electrospinning and optimized for polymer concentration and process parameters. The optimized batch (Trial 5) was prepared by 10% PVA, processed at 22.4 kV applied voltage, and 14 cm plate and spinneret distance to yield thick, uniform, and peelable nanofibers film. There was no interaction observed between Luz and PVA in the FTIR study. DSC and XRD analysis showed that luliconazole was loaded into fabricated nanofibers with a reduced crystallinity. FESEM studies confirmed the smooth, defect-free mats of nanofibers. Luz-PVA nanofibers possessed a tensile strength of 21.8 N and a maximum elongation of 10.8%, representing the excellent elasticity of the scaffolds. For Luz-PVA nanofibers, the sustained and complete drug release was observed in 48 h. In antifungal activity using Candida albicans, the Luz-PVA nanofibers showed a greater zone of inhibition (30.55 ± 0.38 mm and 29.27 ± 0.31 mm) than marketed cream (28.06 ± 0.18 mm and 28.47 ± 0.24 mm) and pure drug (27.57 ± 0.17 mm and 27.50 ± 0.47 mm) at 1% concentration in Sabouraud dextrose agar and yeast malt agar, respectively. Therefore, Luz-PVA nanofibers exhibited good mechanical properties, longer retention time, and better antifungal activity than marketed products and, therefore, can be further examined preclinically as a potential treatment option for topical mycotic infection.

Graphical Abstract

市面上有乳膏或凝胶状的抗真菌剂氟硅唑。这些传统制剂的主要局限是在感染部位的停留时间较短。这项工作的主要目的是开发出用于真菌性皮肤病的具有较长滞留时间的含氟硅唑的聚乙烯醇（Luz-PVA）纳米纤维。通过平板电纺制备了 Luz-PVA 纳米纤维，并对聚合物浓度和工艺参数进行了优化。优化批次（试验 5）采用 10%的 PVA 制备，在 22.4 kV 的应用电压和 14 cm 的板和喷丝板间距下进行处理，以获得厚实、均匀和可剥离的纳米纤维膜。在傅立叶变换红外光谱研究中没有观察到 Luz 和 PVA 之间的相互作用。DSC 和 XRD 分析表明，卢立康唑被负载到制成的纳米纤维中，结晶度降低。FESEM 研究证实了纳米纤维光滑、无缺陷。Luz-PVA 纳米纤维的拉伸强度为 21.8 N，最大伸长率为 10.8%，表明支架具有良好的弹性。在使用白色念珠菌进行抗真菌活性试验时，Luz-PVA 纳米纤维的抑菌区（30.55 ± 0.38 mm 和 29.27 ± 0.31 mm）大于市场上销售的乳膏（29.27 ± 0.31 mm）。31 mm），高于市售乳膏（28.06 ± 0.18 mm 和 28.47 ± 0.24 mm）和纯药物（27.57 ± 0.17 mm 和 27.50 ± 0.47 mm）。因此，与市售产品相比，Luz-PVA 纳米纤维具有良好的机械性能、更长的保留时间和更好的抗真菌活性，因此可作为局部真菌感染的潜在治疗方案进行进一步的临床前研究。

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引用次数: 0

Biomaterial Fg/P(LLA-CL) regulates macrophage polarization and recruitment of mesenchymal stem cells after endometrial injury 生物材料Fg/P(LLA-CL)可调节巨噬细胞极化和子宫内膜损伤后间充质干细胞的招募。

IF 4.2 3区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of Materials Science: Materials in Medicine

Pub Date : 2024-07-29 DOI: 10.1007/s10856-024-06807-w

Sirui Song, Anfeng Wang, Siyu Wu, Huaifang Li, Hongbing He

The process of endometrial repair after injury involves the synergistic action of various cells including immune cells and stem cells. In this study, after combing Fibrinogen(Fg) with poly(L-lacticacid)-co-poly(ε-caprolactone)(P(LLA-CL)) by electrospinning, we placed Fg/P(LLA-CL) into the uterine cavity of endometrium-injured rats, and bioinformatic analysis revealed that Fg/P(LLA-CL) may affect inflammatory response and stem cell biological behavior. Therefore, we verified that Fg/P(LLA-CL) could inhibit the lipopolysaccharide (LPS)-stimulated macrophages from switching to the pro-inflammatory M1 phenotype in vitro. Moreover, in the rat model of endometrial injury, Fg/P(LLA-CL) effectively promoted the polarization of macrophages towards the anti-inflammatory M2 phenotype and enhanced the presence of mesenchymal stem cells at the injury site. Overall, Fg/P(LLA-CL) exhibits significant influence on macrophage polarization and stem cell behavior in endometrial injury, justifying further exploration for potential therapeutic applications in endometrial and other tissue injuries.

Graphical Abstract

子宫内膜损伤后的修复过程涉及免疫细胞和干细胞等多种细胞的协同作用。本研究将纤溶酶原（Fg）与聚（L-乳酸）-共聚（ε-己内酯）（P(LLA-CL)）通过电纺结合后，将Fg/P(LLA-CL)置入子宫内膜损伤大鼠的宫腔，生物信息学分析表明Fg/P(LLA-CL)可能影响炎症反应和干细胞生物学行为。因此，我们在体外验证了Fg/P(LLA-CL)能抑制脂多糖（LPS）刺激的巨噬细胞向促炎的M1表型转换。此外，在大鼠子宫内膜损伤模型中，Fg/P(LLA-CL)能有效促进巨噬细胞向抗炎M2表型极化，并增强损伤部位间充质干细胞的存在。总之，Fg/P(LLA-CL)对子宫内膜损伤中巨噬细胞的极化和干细胞行为有显著影响，值得进一步探索其在子宫内膜和其他组织损伤中的潜在治疗应用。

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引用次数: 0

Biodegradable composites with antibiotics and growth factors for dual release kinetics 含有抗生素和生长因子的生物可降解复合材料的双重释放动力学。

IF 4.2 3区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of Materials Science: Materials in Medicine

Pub Date : 2024-07-29 DOI: 10.1007/s10856-024-06809-8

Michael Seidenstuecker, Julian Hess, Anna Baghnavi, Hagen Schmal, Diana Voigt, Hermann O. Mayr

Bone infections are still a major problem in surgery. To avoid severe side effects of systemically administered antibiotics, local antibiotic therapy is increasingly being considered. Using a pressure-based method developed in our group, microporous β-TCP ceramics, which had previously been characterized, were loaded with 2% w/v alginate containing 50 mg/mL clindamycin and 10 µg/mL rhBMP-2. Release experiments were then carried out over 28 days with changes of liquid at defined times (1, 2, 3, 6, 9, 14, 21 and 28d). The released concentrations of clindamycin were determined by HPLC and those of rhBMP-2 by ELISA. Continuous release (anomalous transport) of clindamycin and uniform release (Fick’s diffusion) of BMP-2 were determined. The composites were biocompatible (live/dead, WST-I and LDH) and the released concentrations were all antimicrobially active against Staph. aureus. The results were very promising and clindamycin was detected in concentrations above the MIC as well as a constant rhBMP-2 release over the entire study period. Biocompatibility was also not impaired by either the antibiotic or the BMP-2. This promising approach can therefore be seen as an alternative to the common treatment with PMMA chains containing gentamycin, as the new composite is completely biodegradable and no second operation is necessary for removal or replacement.

Graphical Abstract

骨感染仍然是外科手术中的一个主要问题。为了避免全身使用抗生素的严重副作用，越来越多的人开始考虑局部抗生素治疗。利用我们小组开发的一种基于压力的方法，用含有 50 毫克/毫升克林霉素和 10 微克/毫升 rhBMP-2 的 2% w/v 海藻酸盐装载了微孔 β-TCP 陶瓷。然后在规定的时间（1、2、3、6、9、14、21 和 28 天）更换液体，进行 28 天的释放实验。通过 HPLC 测定克林霉素的释放浓度，通过 ELISA 测定 rhBMP-2 的释放浓度。测定了克林霉素的持续释放（反常运输）和 BMP-2 的均匀释放（菲克扩散）。复合材料具有生物相容性（活/死、WST-I 和 LDH），释放浓度对金黄色葡萄球菌均有抗菌活性。研究结果非常乐观，检测到克林霉素的浓度高于 MIC，而且在整个研究期间，rhBMP-2 的释放量保持稳定。生物相容性也没有受到抗生素或 BMP-2 的影响。这种新型复合材料可完全生物降解，无需进行二次手术即可取出或更换，因此可作为使用含有庆大霉素的 PMMA 链的常见治疗方法的替代方法。

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引用次数: 0

Adverse effects of sterilization processes on the fundamental topographic properties of modified dental implant surfaces 消毒过程对改良牙科种植体表面基本形貌特性的不利影响。

IF 4.2 3区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of Materials Science: Materials in Medicine

Pub Date : 2024-07-29 DOI: 10.1007/s10856-024-06813-y

Marcel F. Kunrath, Roberto Hubler, Christer Dahlin

The employ of sterilization processes are essential to investigate biomaterials aiming for experimental, preclinical, or clinical applications with biological tissues. However, responsive surface properties of biomaterials may be susceptible to sterilization processes, compromising important physio-chemical characteristics. For that reason, this in vitro study aimed to investigate the effects of three different processes for sterilization (humid heat under pressure, UVC-light exposure, and Gamma irradiation) on the major topographical properties of implant surfaces applied to dental bone-anchored implants and/or implant-abutments. Three groups of implant surfaces were developed: a smooth machined surface, a micro-texturized surface, and a hydrophilic micro-texturized surface. The implants were sterilized with three methodologies and characterized regarding surface morphology, elemental surface composition, roughness parameters, wettability characteristics, and compared to the samples as-developed. Surface morphology and roughness parameters were not modified by any of the sterilization processes applied. On the other hand, hydrophilic implants were negatively affected by autoclaving. After package opening, hydrophilic features showed to be sensible to atmospheric air exposition independently of the sterilization process performed. Our findings revealed significant chemical changes on the implant surfaces caused by autoclaving and UVC exposure; additionally, the results showed the importance of selecting an appropriate sterilization method when investigating hydrophilic implants so as not to generate imprecise outcomes.

Graphical Abstract

采用灭菌工艺对研究生物组织实验、临床前或临床应用的生物材料至关重要。然而，生物材料的表面响应特性很容易受到灭菌过程的影响，从而损害重要的物理化学特性。因此，这项体外研究旨在调查三种不同灭菌工艺（加压湿热、紫外线照射和伽马射线照射）对应用于牙科骨固定种植体和/或种植基台的种植体表面主要地形特性的影响。我们开发了三组种植体表面：光滑机加工表面、微纹理表面和亲水性微纹理表面。种植体采用三种方法进行灭菌，并对表面形态、元素表面成分、粗糙度参数、润湿性特征进行表征，并与开发时的样品进行比较。表面形态和粗糙度参数未因任何灭菌过程而改变。另一方面，亲水性植入物受到高压灭菌的负面影响。包装打开后，亲水性特征显示出对大气暴露的敏感性，与所执行的灭菌过程无关。我们的研究结果表明，高压灭菌和紫外线照射会导致植入体表面发生明显的化学变化；此外，研究结果还表明，在研究亲水性植入体时，选择适当的灭菌方法非常重要，以免产生不精确的结果。

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引用次数: 0

Transdermal delivery of botulinum toxin-A through phosphatidylcholine/cholesterol nanoliposomes for treatment of post-acne scarring 通过磷脂酰胆碱/胆固醇纳米脂质体透皮输送肉毒杆菌毒素-A，用于治疗痤疮后瘢痕。

IF 4.2 3区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of Materials Science: Materials in Medicine

Pub Date : 2024-07-29 DOI: 10.1007/s10856-024-06810-1

Lannan Chen, Lei Cui, Jiabing Ran, Zhengrui Liu, Xiongbin Zhu

As an acne sequela, post-acne scarring (PSA) has huge negative impact on sufferers’ quality of life because of aesthetical embarrassment. Transdermal delivery of botulinum toxin-A (BTXA) is a promising strategy for PAS treatment, but currently reported approaches are far from satisfactory. In this work, phosphatidylcholine/cholesterol (PC/Chol) nanoliposomes were utilized for encapsulation and transdermal delivery of BTXA. The composition, structure, morphology, size, size distribution, etc. of as-prepared BTXA@liposome nanoparticles were investigated in detail. Simulated transdermal delivery assay indicated that the diffusion depth of the BXTA@liposome nanoparticles was nearly 8 times that of pure BTXA and reached 380 μm. 12 facial PSA patients were recruited to evaluate the curative effect of the BTXA@liposome nanoparticles on PSA. Through ECCA (échelle d’évaluation clinique des cicatrices d’acné) scoring and self-evaluation of patients, the resultant data indicated that compared to hyaluronic acid (HA) hydrogel treatment the BTXA@liposome/HA hydrogel treatment could better relieve PSA to some extent but didn’t show significant advantage. Further work is needed to verify the feasibility and curative effect of this method in PSA treatment in the future.

Graphical Abstract

作为痤疮的后遗症，痤疮后瘢痕（PSA）会给患者带来美观上的尴尬，从而对其生活质量产生巨大的负面影响。透皮给药肉毒素-A（BTXA）是治疗痤疮后瘢痕的一种很有前景的方法，但目前报道的方法还远远不能令人满意。本研究利用磷脂酰胆碱/胆固醇（PC/Chol）纳米脂质体来封装和透皮给药 BTXA。详细研究了制备的 BTXA@脂质体纳米颗粒的组成、结构、形态、大小、粒度分布等。模拟透皮给药试验表明，BXTA@脂质体纳米颗粒的扩散深度是纯BTXA的近8倍，达到380 μm。为了评估 BTXA@脂质体纳米粒子对 PSA 的治疗效果，研究人员招募了 12 名面部 PSA 患者。通过ECCA（Echelle d'évaluation clinique des cicatrices d'acné）评分和患者自我评估，结果数据显示，与透明质酸（HA）水凝胶治疗相比，BTXA@脂质体/HA水凝胶治疗能在一定程度上更好地缓解PSA，但并没有显示出明显的优势。未来还需要进一步验证该方法在 PSA 治疗中的可行性和疗效。

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引用次数: 0

Design and characterization of electroactive gelatin methacrylate hydrogel incorporated with gold nanoparticles empowered with parahydroxybenzaldehyde and curcumin for advanced tissue engineering applications 设计并表征加入对羟基苯甲醛和姜黄素的金纳米颗粒的电活性甲基丙烯酸明胶水凝胶，用于先进的组织工程应用。

IF 4.2 3区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of Materials Science: Materials in Medicine

Pub Date : 2024-07-29 DOI: 10.1007/s10856-024-06808-9

Zahra Barabadi, Asrin Bahmani, Marzieh Jalalimonfared, Milad Ashrafizadeh, Morteza Rashtbar, Esmaeel Sharifi, Haili Tian

Electroconductive polymers are the materials of interest for the fabrication of electro-conductive tissues. Metal ions through the redox systems offer polymers with electrical conductivity. In this study, we processed a gelatin methacrylate (GelMA) network with gold nanoparticles (GNPs) through a redox system with parahydroxybenzaldehyde (PHB) or curcumin to enhance its electrical conductivity. Induction of the redox system with both PHB and curcumin into the GelMA, introduced some new functional groups into the polymeric network, as it has been confirmed by H-NMR and FTIR. These new bonds resulted in higher electro-conductivity when GNPs were added to the polymer. Higher electroactivity was achieved by PHB compared to the curcumin-induced redox system, and the addition of GNPs without redox system induction showed the lowest electroactivity. MTT was used to evaluate the biocompatibility of the resultant polymers, and the PHB-treated hydrogels showed higher proliferative effects on the cells. The findings of this study suggest that the introduction of a redox system by PHB in the GelMA network along with GNPs can contribute to the electrochemical properties of the material. This electroactivity can be advantageous for tissue engineering of electro-conductive tissues like cardiac and nervous tissues.

Graphical Abstract

导电聚合物是制造导电组织的理想材料。金属离子通过氧化还原系统使聚合物具有导电性。在本研究中，我们通过对羟基苯甲醛（PHB）或姜黄素的氧化还原体系处理了含有金纳米粒子（GNPs）的甲基丙烯酸明胶（GelMA）网络，以增强其导电性。经 H-NMR 和 FTIR 证实，在 GelMA 中同时加入 PHB 和姜黄素的氧化还原体系会在聚合物网络中引入一些新的官能团。在聚合物中添加 GNP 时，这些新键可产生更高的电导率。与姜黄素诱导的氧化还原体系相比，PHB 具有更高的电导率，而添加 GNPs 但不诱导氧化还原体系的电导率最低。用 MTT 评估了所得聚合物的生物相容性，PHB 处理过的水凝胶对细胞的增殖效果较高。本研究的结果表明，PHB 与 GNP 一起在 GelMA 网络中引入氧化还原系统，有助于提高材料的电化学特性。这种电活性有利于心脏和神经组织等导电组织的组织工程。

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引用次数: 0

Identification of key genes involved in collagen hydrogel-induced chondrogenic differentiation of mesenchymal stem cells through transcriptome analysis: the role of m6A modification 通过转录组分析鉴定参与胶原水凝胶诱导间充质干细胞软骨分化的关键基因：m6A修饰的作用。

IF 4.2 3区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of Materials Science: Materials in Medicine

Pub Date : 2024-07-29 DOI: 10.1007/s10856-024-06801-2

Chaotao Chen, Kai Xiong, Kanglu Li, Bo Zhou, Jianwen Cheng, Bo Zhu, Li Zheng, Jinmin Zhao

Collagen hydrogel has been shown promise as an inducer for chondrogenic differentiation of bone marrow mesenchymal stem cells (BMSCs), contributing to the repair of cartilage defects. However, the precise molecular mechanism underlying this phenomenon remains poorly elucidated. Here, we induced chondrogenic differentiation of BMSCs using collagen hydrogel and identified 4451 differentially expressed genes (DEGs) through transcriptomic sequencing. Our analysis revealed that DEGs were enriched in the focal adhesion pathway, with a notable decrease in expression levels in the collagen hydrogel group compared to the control group. Protein-protein interaction network analysis suggested that actinin alpha 1 (ACTN1) and actinin alpha 4 (ACTN4), two proteins also involved in cytoskeletal recombination, may be crucial in collagen hydrogel-induced chondrogenic differentiation of BMSCs. Additionally, we found that N6-methyladenosine RNA methylation (m6A) modification was involved in collagen hydrogel-mediated chondrogenic differentiation, with fat mass and obesity-associated protein (FTO) implicated in regulating the expression of ACTN1 and ACTN4. These findings suggest that collagen hydrogel might regulate focal adhesion and actin cytoskeletal signaling pathways through down-regulation of ACTN1 and ACTN4 mRNA via FTO-mediated m6A modification, ultimately driving chondrogenic differentiation of BMSCs. In conclusion, our study provides valuable insights into the molecular mechanisms of collagen hydrogel-induced chondrogenic differentiation of BMSCs, which may aid in developing more effective strategies for cartilage regeneration.

Graphical Abstract

胶原水凝胶作为骨髓间充质干细胞（BMSCs）软骨分化的诱导剂，有助于软骨缺损的修复。然而，这一现象背后的确切分子机制仍未阐明。在这里，我们使用胶原水凝胶诱导了骨髓造血干细胞的软骨分化，并通过转录组测序鉴定了4451个差异表达基因（DEGs）。我们的分析表明，DEGs富集于局灶粘附通路，与对照组相比，胶原水凝胶组的表达水平明显下降。蛋白-蛋白相互作用网络分析表明，肌动蛋白α1（ACTN1）和肌动蛋白α4（ACTN4）这两种也参与细胞骨架重组的蛋白可能在胶原水凝胶诱导的BMSCs软骨分化中起着关键作用。此外，我们还发现 N6-甲基腺苷 RNA 甲基化（m6A）修饰参与了胶原水凝胶介导的软骨分化，脂肪量和肥胖相关蛋白（FTO）与 ACTN1 和 ACTN4 的表达调控有关。这些发现表明，胶原水凝胶可能通过 FTO 介导的 m6A 修饰下调 ACTN1 和 ACTN4 mRNA，从而调节病灶粘附和肌动蛋白细胞骨架信号通路，最终驱动 BMSCs 的软骨分化。总之，我们的研究为胶原水凝胶诱导 BMSCs 软骨分化的分子机制提供了有价值的见解，有助于开发更有效的软骨再生策略。

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