Journal of Materials Science: Materials in Medicine最新文献_第3页

Degraded products generated by iron stent inhibit the vascular smooth muscle cell proliferation by downregulating AP-1 铁支架降解产物通过下调AP-1抑制血管平滑肌细胞增殖

IF 4.2 3区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of Materials Science: Materials in Medicine

Pub Date : 2025-01-13 DOI: 10.1007/s10856-024-06854-3

Jiabing Huang, Bingjian Liu, Chunguang Zhao, Jing Li, Dongxu Qiu

In-stent restenosis (ISR) following interventional therapy is a fatal clinical complication. Current evidence indicates that neointimal hyperplasia driven by uncontrolled proliferation of vascular smooth muscle cells (VSMC) is a major cause of restenosis. This implies that inhibiting VSMC proliferation may be an attractive approach for preventing in-stent restenosis. In our previous study, we found that the iron stent reduced the neointimal hyperplasia in an atherosclerotic artery stenosis model, and the iron corroded granules generated by the iron stent inhibited neointimal hyperplasia by suppressing the proliferation of VSMCs. However, this observation needs to be validated through in vitro experimentation. In this study, co-culture experiments and flow cytometer assays were performed to qualitatively investigate the effects of iron stent degradation on VSMCs. Moreover, the degraded products resulting generated by the iron stent were collected and used to elucidate the suppressive effect of the iron stents. The underlying mechanism was explored through molecular biology assays. The major findings are as follows: 1) The degraded iron stent inhibited the proliferation of VSMCs; 2) The degraded products of the iron stent downregulated the expression of AP-1. In summary, this study demonstrates the inhibitory effect of degraded iron products on VSMC proliferation, implying that such products have the potential to mitigate in-stent restenosis.

Graphical Abstract

degraded products generated from iron stent inhibit the vascular smooth muscle cell proliferation by downregulating AP-1.

介入治疗后支架内再狭窄（ISR）是一种致命的临床并发症。目前的证据表明，由血管平滑肌细胞（VSMC）不受控制的增殖驱动的新生内膜增生是再狭窄的主要原因。这意味着抑制VSMC增殖可能是预防支架内再狭窄的一种有吸引力的方法。在我们之前的研究中，我们发现铁支架可以减少动脉粥样硬化性狭窄模型的新生内膜增生，铁支架产生的铁腐蚀颗粒通过抑制VSMCs的增殖来抑制新生内膜增生。然而，这一观察结果需要通过体外实验来验证。本研究通过共培养实验和流式细胞仪检测，定性探讨铁支架降解对VSMCs的影响。此外，还收集了铁支架产生的降解产物，并用于阐明铁支架的抑制作用。通过分子生物学分析探讨其潜在机制。主要结果如下：1)降解铁支架抑制VSMCs的增殖；2)铁支架降解产物下调AP-1的表达。综上所述，本研究证明了降解铁产物对VSMC增殖的抑制作用，这意味着这些产物具有减轻支架内再狭窄的潜力。铁支架降解产物通过下调AP-1抑制血管平滑肌细胞增殖。

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引用次数: 0

Protective effect of quercetin loaded on bifunctional periodic mesoporous organosilica against damage induced by irradiation on the male reproductive system 槲皮素负载双功能介孔二氧化硅对辐照对男性生殖系统损伤的保护作用

IF 4.2 3区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of Materials Science: Materials in Medicine

Pub Date : 2025-01-13 DOI: 10.1007/s10856-024-06857-0

S. F. Mirtaheri, S. N. Mousavi, Z. Abdi, E. Hosseini, M. S. Seyed Dorraji, F. Kabiri Esfahani, M. Gholami

Preserving fertility is important in men under radiation therapy because healthy cells are also affected by radiation. Supplementation with antioxidants is a controversial issue in this process. Designing a biocompatible delivery system containing hydrophobic antioxidants to release control may solve these disagreements. Herein, a bifunctional periodic mesoporous organosilica (PMO) was designed to load quercetin (Quer) and its application was studied on damaged cells induced by irradiation on the male reproductive system. Quercetin-loaded on PMO significantly improved the length and width of the testis after irradiation compared to the Quer, alone (p < 0.001 and p < 0.001, respectively). Sperm viability was significantly higher in the Quer-loaded on PMO than Quer, alone after irradiation (p < 0.001). Irradiation significantly decreased the sperm count (p = 0.01), however, Quer and Quer-loaded on PMO could not increase them to the normal ranges. Quer alone and loaded on PMO significantly reduced the sperm with abnormal morphology after irradiation (p < 0.001). Quer alone, and loaded on PMO significantly increased daily production of sperm after irradiation (p < 0.001). The number of apoptotic cells significantly increased after irradiation (p < 0.001). After irradiation, Quer loaded on PMO significantly decreased the apoptotic cells compared to the irradiated (p < 0.001) and Quer, alone groups (p < 0.001). The novel synthesized PMO containing Quer reduced the side effects of irradiation on the male reproductive system.

Graphical Abstract

对于接受放射治疗的男性来说，保持生育能力很重要，因为健康细胞也会受到辐射的影响。在这个过程中补充抗氧化剂是一个有争议的问题。设计一种含有疏水性抗氧化剂的生物相容性释放系统可以解决这些分歧。本文设计了一种双功能周期介孔有机硅（PMO）来负载槲皮素（Quer），并研究了其在辐照诱导的男性生殖系统损伤细胞中的应用。与单独使用Quer相比，PMO上负载槲皮素显著改善了照射后睾丸的长度和宽度（分别为p <； 0.001和p <； 0.001）。在PMO上装载Quer的精子活力明显高于单独照射后的Quer （p < 0.001）。辐照显著降低了精子数量（p = 0.01），而Quer和PMO上的Quer不能使精子数量增加到正常范围。单用Quer和PMO加载可显著减少辐照后形态异常的精子（p < 0.001）。单独使用Quer和加载PMO显著增加辐照后精子的日生成量（p < 0.001）。辐照后凋亡细胞数量显著增加（p < 0.001）。照射后，与照射组（p < 0.001）和单独照射组（p < 0.001）相比，Quer负载PMO显著减少了凋亡细胞。新型合成的含Quer的PMO减少了辐照对男性生殖系统的副作用。图形抽象

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引用次数: 0

Enhanced bone cement for fixation of prosthetic joint utilizing nanoparticles 利用纳米颗粒增强骨水泥固定假体关节

IF 4.2 3区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of Materials Science: Materials in Medicine

Pub Date : 2025-01-13 DOI: 10.1007/s10856-024-06848-1

Safaa Gamal, Mina Mikhail, Nancy Salem, Mohamed Tarek EL-Wakad, Reda Abdelbaset

Bone cement is commonly utilized to secure prosthetic joints in the body because of its robust fixation, stability, biocompatibility, and immediate load-bearing capability. However, issues such as loosening, leakage, and insufficient bioactivity can lead to its failure. Therefore, improving its mechanical, physical, and biological properties is crucial for enhancing its efficiency. This study examines the impact of incorporating four different nanomaterials—Titanium Dioxide (TiO₂), Magnesium Oxide (MgO), Calcium Phosphate (Ca₃(PO₄)₂), and Alumina Oxide (Al₂O₃)—into bone cement on its mechanical, physical, and biological properties. TiO₂ and Al₂O₃ nanoparticles are selected to enhance the compression strength of bone cement, thereby preventing loosening. Magnesium Oxide (MgO) and Ca₃(PO₄)₂ nanoparticles are chosen to improve cell adhesion and reducing the risk of cement leakage. Five specimens were prepared: the first with 100% pure bone cement powder, the second with 98% pure bone cement powder and modified with 2% MgO and TiO2, and the remaining three with 95% pure bone cement powder and modified with 5% varying ratios of MgO, TiO₂, Ca₃(PO₄)₂, and Al₂O₃. Compression, tensile, hardness, and bending strengths were assessed to determine improvements in mechanical properties. Setting temperature, porosity, and degradation were measured to evaluate physical properties. Cell adhesion and toxicity tests were conducted to examine the surface structure and biological properties. The results demonstrated that the modified specimens increased compression strength by 8.14%, tensile strength by 3.4%, and bending strength by 4.96%. Porosity, degradation, and setting temperature in modified specimens increased by 3.24%, 0.64%, and 5.17% respectively pure bone cement values. Cell adhesion in modified bone cement specimens showed normal attachment when scanned with FE-SEM. All of the tested modified specimens showed no toxicity, except for specimens with 2% Al₂O₃ that showed 25% toxicity which could be averted by employing antibiotics.

Graphical Abstract

骨水泥因其牢固的固定、稳定性、生物相容性和即时承重能力而被广泛用于人体假体关节的固定。然而，诸如松动、渗漏和生物活性不足等问题可能导致其失效。因此，提高其机械、物理和生物性能是提高其效率的关键。本研究考察了将四种不同的纳米材料——二氧化钛（TiO2）、氧化镁（MgO）、磷酸钙（Ca3(PO4)2）和氧化铝（Al2O3）——加入骨水泥对其机械、物理和生物性能的影响。选择TiO2和Al2O3纳米颗粒来提高骨水泥的抗压强度，从而防止骨水泥松动。选择氧化镁（MgO）和Ca3(PO4)2纳米颗粒来提高细胞粘附性，降低水泥泄漏的风险。制备了5个标本：第一个为100%纯骨水泥粉，第二个为98%纯骨水泥粉，用2% MgO和TiO2改性，其余3个为95%纯骨水泥粉，用5%不同比例的MgO、TiO2、Ca3(PO4)2和Al2O3改性。对压缩、拉伸、硬度和抗弯强度进行了评估，以确定机械性能的改善。通过测量凝固温度、孔隙度和降解度来评估物理性能。进行了细胞粘附和毒性试验，以检验其表面结构和生物学特性。结果表明，改性后的试样抗压强度提高了8.14%，抗拉强度提高了3.4%，抗弯强度提高了4.96%。改性后的孔隙率、降解率和凝固温度分别比纯骨水泥提高3.24%、0.64%和5.17%。在FE-SEM扫描下，细胞粘附正常。除含有2% Al2O3的样品显示25%的毒性外，所有被测试的改性样品均无毒性，这可以通过使用抗生素来避免。图形抽象

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引用次数: 0

Insights on the role of cryoprotectants in enhancing the properties of bioinks required for cryobioprinting of biological constructs 低温保护剂在提高生物结构的低温打印所需的生物墨水性能方面的作用

IF 4.2 3区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of Materials Science: Materials in Medicine

Pub Date : 2025-01-13 DOI: 10.1007/s10856-024-06855-2

Harshavardhan Budharaju, Dhakshinamoorthy Sundaramurthi, Swaminathan Sethuraman

Preservation and long-term storage of readily available cell-laden tissue-engineered products are major challenges in expanding their applications in healthcare. In recent years, there has been increasing interest in the development of off-the-shelf tissue-engineered products using the cryobioprinting approach. Here, bioinks are incorporated with cryoprotective agents (CPAs) to allow the fabrication of cryopreservable tissue constructs. Although this method has shown potential in the fabrication of cryopreservable tissue-engineered products, the impact of the CPAs on the viscoelastic behavior and printability of the bioinks at cryo conditions remains unexplored. In this study, we have evaluated the influence of CPAs such as glycerol and dimethyl sulfoxide (DMSO) on the rheological properties of pre-crosslinked alginate bioinks for cryoprinting applications. DMSO-incorporated bioinks showed a reduction in viscosity and yield stress, while the addition of glycerol improved both the properties due to interactions with the calcium chloride used for pre-crosslinking. Further, tube inversion and printability experiments were performed to identify suitable concentrations and cryobioprinting conditions for bioinks containing CPAs & pre-crosslinked with CaCl₂. Finally, based on the printability analysis & cell recovery results, 10% glycerol was used for cryobioprinting and preservation of cell-laden constructs at −80 °C and the viability of cells within the printed structures were evaluated after recovery. Cell viability results indicate that the addition of 10% glycerol to the pre-crosslinked bioink significantly improved cell viability compared to bioinks without CPAs, confirming the suitability of the developed bioink combination to fabricate tissue constructs for on-demand applications.

Graphical abstract

Effect of cryoprotectants on the viscoelastic behavior of bioinks and cell recovery in cryobioprinted tissue constructs.

保存和长期储存现成的细胞负载组织工程产品是扩大其在医疗保健中的应用的主要挑战。近年来，人们对使用冷冻生物打印方法开发现成的组织工程产品越来越感兴趣。在这里，生物墨水与低温保护剂（cpa）结合，以允许制造可低温保存的组织结构。尽管这种方法在制造可低温保存的组织工程产品方面显示出潜力，但在低温条件下，cpa对生物墨水的粘弹性行为和可打印性的影响仍未被探索。在这项研究中，我们评估了CPAs如甘油和二甲基亚砜（DMSO）对预交联海藻酸盐生物墨水的流变性能的影响。加入dmso的生物墨水显示出粘度和屈服应力的降低，而甘油的加入由于与用于预交联的氯化钙相互作用而改善了这两种性能。此外，进行了试管倒置和可打印性实验，以确定含cpa的生物墨水的合适浓度和低温打印条件；与CaCl2预交联。最后，在印刷适性分析的基础上；细胞恢复结果，使用10%甘油进行冷冻打印，并在−80°C下保存细胞负载结构，并在恢复后评估打印结构内细胞的活力。细胞活力结果表明，与不添加CPAs的生物墨水相比，在预交联生物墨水中添加10%甘油可显著提高细胞活力，证实了所开发的生物墨水组合在按需应用中制造组织构建物的适用性。低温保护剂对低温生物打印组织结构中生物墨水粘弹性行为和细胞恢复的影响。

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引用次数: 0

Zinc oxide nanoparticles decorated nitrogen doped porous reduced graphene oxide-based hybrid to sensitive detection of hydroxychloroquine in plasma and urine 氧化锌纳米粒子修饰氮掺杂多孔还原氧化石墨烯基杂化物对血浆和尿液中羟氯喹的灵敏检测

IF 4.2 3区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of Materials Science: Materials in Medicine

Pub Date : 2025-01-07 DOI: 10.1007/s10856-024-06847-2

Mohammad Amiri, Zahra Hashemi, Fereshteh Chekin

The antimalarial hydroxychloroquine (HCQ) has considered for the treatment of systemic lupus erythematosus. Moreover, HCQ has been used as a drug to treat Coronavirus disease (COVID-19). In this work, nitrogen doped porous reduced graphene oxide (NprGO) has been prepared via environmentally friendly process using Fummaria Parviflora extract. A catalyst based on ZnO nanoparticles-nitrogen doped porous reduced graphene oxide (ZnO-NprGO) was prepared by hydrothermal method and characterized. The diameter of ZnO nanoparticles was ~22–37 nm, which were inserted between the NprGO sheets effectively prevented their aggregation. The ZnO-NprGO hybrid had high surface area and good electro-catalytic property, suiting for determination of HCQ. The ZnO-NprGO modified carbon paste electrode (CPE)-based sensor operated in a wide concentration range of 0.07–5.5 μmol L⁻¹ with low limit of detection of 57 nmol L⁻¹ and sensitivity of 14.175 μA μmol⁻¹ L. Remarkably, the ZnO-NprGO/CPE sensor indicated acceptable accuracy, reproducibility, and stability. In addition, the proposed sensor was applied to detection of HCQ in biological samples and the recoveries were 92.0–102.5%, with relative standard deviations of 1.9–4.3%. The unique physical structure of ZnO-NprGO, as well as its chemical and electrical properties, make it promising interface for use in sensors and nanoelectronic applications.

Graphical Abstract

抗疟药羟氯喹（HCQ）已被考虑用于治疗系统性红斑狼疮。此外，HCQ已被用作治疗冠状病毒病（COVID-19）的药物。本研究采用环保工艺，以小檗提取物为原料制备了氮掺杂多孔还原氧化石墨烯（NprGO）。采用水热法制备了ZnO纳米颗粒-氮掺杂多孔还原性氧化石墨烯（ZnO- nprgo）催化剂，并对其进行了表征。ZnO纳米颗粒的直径为~22 ~ 37 nm，嵌入在NprGO薄片之间有效地阻止了它们的聚集。ZnO-NprGO杂化物具有高的比表面积和良好的电催化性能，适用于HCQ的测定。基于ZnO-NprGO修饰碳糊电极（CPE）的传感器工作范围为0.07 ~ 5.5 μmol L−1，检测下限为57 μmol L−1，灵敏度为14.175 μA μmol−1 L，具有良好的准确度、重复性和稳定性。该传感器可用于生物样品中HCQ的检测，加样回收率为92.0 ~ 102.5%，相对标准偏差为1.9 ~ 4.3%。ZnO-NprGO独特的物理结构，以及它的化学和电学性质，使其在传感器和纳米电子应用中具有前景。图形抽象

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引用次数: 0

Knockdown of decorin in human bone marrow mesenchymal stem cells suppresses proteoglycan layer formation and establishes a pro-inflammatory environment on titanium oxide surfaces 人骨髓间充质干细胞中decorin的敲低抑制蛋白聚糖层的形成并在氧化钛表面建立促炎环境

IF 4.2 3区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of Materials Science: Materials in Medicine

Pub Date : 2025-01-07 DOI: 10.1007/s10856-024-06849-0

Hisanobu Kamio, Kazuto Okabe, Masaki Honda, Kensuke Kuroda, Shuhei Tsuchiya

Osseointegration is essential for successful implant treatment. However, the underlying molecular mechanisms remain unclear. In this study, we focused on decorin (DCN), which was hypothesized to be present in the proteoglycan (PG) layer at the interface between bone and the titanium oxide (TiOx) surface. We utilized DCN RNA interference in human bone marrow mesenchymal stem cells (hBMSCs) to investigate its effects on PG layer formation, proliferation, initial adhesion, cell extension, osteogenic capacity, fibrotic markers, and immunotolerance to TiOx in vitro. After 14 days of cultivation, we observed no PG layer was detected, and the osteogenic capacity was suppressed in DCN-depleted hBMSCs. Furthermore, the conditioned medium upregulated the expression of M1 macrophage markers in human macrophages. These results suggest that endogenous DCN plays a crucial role in PG layer formation and that the PG layer alters inflammation around Ti materials.

Graphical Abstract

骨整合是成功种植体治疗的关键。然而，潜在的分子机制尚不清楚。在这项研究中，我们重点研究了decorin (DCN)，它被假设存在于骨和氧化钛（TiOx）表面交界面的蛋白聚糖（PG）层中。我们利用DCN RNA干扰人骨髓间充质干细胞（hBMSCs），研究其对PG层形成、增殖、初始粘附、细胞扩展、成骨能力、纤维化标志物和体外对TiOx免疫耐受的影响。培养14天后，我们观察到未检测到PG层，并且在dcn缺失的hBMSCs中，成骨能力受到抑制。此外，条件培养基上调了人巨噬细胞中M1巨噬细胞标志物的表达。这些结果表明，内源性DCN在PG层的形成中起着至关重要的作用，PG层改变了Ti材料周围的炎症。图形抽象

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引用次数: 0

Understanding the relationship between pore structure and properties of triply periodic minimal surface bone scaffolds 三周期最小表面骨支架孔结构与性能关系的研究

IF 4.2 3区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of Materials Science: Materials in Medicine

Pub Date : 2025-01-07 DOI: 10.1007/s10856-024-06856-1

Yadi Sun, Yan Wang, Benchao Dong, Peichuan Yang, Chunhui Ji, Yiyang Li, Jianxiong Ma, Xinlong Ma

The number of patients with bone defects caused by trauma and diseases has been increasing year by year. The treatment of bone defects remains a major challenge in clinical practice. Bone scaffolds are increasingly favored for repairing bones, with triply periodic minimal surface (TPMS) scaffolds emerging as a popular option due to their superior performance. The aim of this review is to highlight the crucial influence of pore structure on the properties of TPMS bone scaffolds, offering important insights for their innovation and production. It briefly examines various elements that influence the properties of TPMS bone scaffolds, such as pore shape, porosity, pore diameter, and curvature. By analyzing these elements, this review serves as a valuable reference for upcoming research and practical implementations in the field of bone tissue engineering.

Graphical Abstract

创伤和疾病引起的骨缺损患者逐年增加。骨缺损的治疗仍然是临床实践中的一个重大挑战。骨支架在骨修复中越来越受到青睐，三周期最小表面（TPMS）支架由于其优越的性能而成为一种流行的选择。本文综述的目的是强调孔隙结构对TPMS骨支架性能的重要影响，为其创新和生产提供重要见解。它简要地检查了影响TPMS骨支架性能的各种因素，如孔隙形状、孔隙度、孔径和曲率。通过对这些因素的分析，为今后骨组织工程领域的研究和实际应用提供有价值的参考。图形抽象

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引用次数: 0

Strontium nitrate-dopped zinc oxide-loaded alginate gels with gentamicin for improved wound healing 硝酸锶掺杂氧化锌负载海藻酸盐凝胶与庆大霉素促进伤口愈合

IF 4.2 3区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of Materials Science: Materials in Medicine

Pub Date : 2025-01-07 DOI: 10.1007/s10856-024-06836-5

Suhair Hikmat, Ola Tarawneh, Lama Hamadneh, Rania Hamed, Ala A. Alhusban, Mohammad Hailat, Hadeel Abu Mahfouz, Sawsan Shraim, Alghadeer Al-Shammari, Aya Aljariri, Rafa Abu Rayya, Lana Hamdan

Wound dressing development is an area of active research. Traditional dressings lack antibacterial activity, biocompatibility, and tissue regeneration. Alginate is a heavily investigated polymer employed as wound dressings and can be combined with a wide range of additives. Herein, we report the preparation of alginate gel using the crosslinking technique as potential wound dressing, with insight investigation of the influence of employing single, two, or three cross-linkers: Strontium (Sr), zinc oxide (ZnO), and gentamicin sulfate. Rheology was used to confirm the gel’s preparation, where the samples’ viscosity curves show decreased viscosity with increased shear rate, indicating pseudoplastic flow. The linear viscoelastic region shows constant G’ and G” within the sample structure. In this study, we used three gels with different mixtures of ingredients: Gels A, B, and C contain sodium alginate (1% w/v) and 0.5 mL of Sr nitrate (4% w/v). However, Gels B and C contain 0.25 mL of ZnO (0.5% w/v). Gel C also includes 0.1 mL of gentamicin (1% w/v). The study examined the effectiveness of Gel A, B, and C on wound healing, calculating the reduction of wound area after seven, 14, and 20 days of a single topical treatment. Gel A, B, and C significantly reduced wound area, while Gel B and C showed a significant reduction. The zone of inhibition was used to detect the gels’ efficacy against microorganisms. The study found zinc deposition in the liver and bone, with Gel B and C showing higher levels. The study also found significant overexpression of MIP α and MIP β in tissues and downregulation of CCL2, IL8, and TGF β, explaining wound healing with minimal scar formation.

Graphical Abstract

创面敷料的发展是一个活跃的研究领域。传统敷料缺乏抗菌活性、生物相容性和组织再生能力。海藻酸盐是一种被广泛研究的聚合物，用于伤口敷料，可以与多种添加剂结合使用。在此，我们报道了使用交联技术制备海藻酸盐凝胶作为潜在的伤口敷料，并深入研究了使用单一，两种或三种交联剂：锶（Sr），氧化锌（ZnO）和硫酸庆大霉素的影响。用流变学来证实凝胶的制备，样品的粘度曲线显示粘度随着剪切速率的增加而降低，表明假塑性流动。线性粘弹性区在试样结构内呈现恒定的G′和G”。在这项研究中，我们使用了三种不同成分混合物的凝胶：凝胶A， B和C含有海藻酸钠（1% w/v）和0.5 mL硝酸锶（4% w/v）。而凝胶B和C则含有0.25 mL （0.5% w/v）的ZnO。凝胶C还含有0.1 mL庆大霉素（1% w/v）。研究考察了凝胶A、B和C对伤口愈合的有效性，计算了单次局部治疗7天、14天和20天后伤口面积的减少。凝胶A、B、C明显减少创面面积，凝胶B、C明显减少创面面积。用抑菌带检测凝胶的抑菌效果。研究发现锌沉积在肝脏和骨骼中，凝胶B和C的含量更高。该研究还发现MIP α和MIP β在组织中显著过表达，CCL2、IL8和TGF β下调，解释了伤口愈合时瘢痕形成最少的原因。图形抽象

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引用次数: 0

Histological and histomorphometric evaluation of natural bovine bone substitute with hyaluronate in socket preservation—a report of three cases 含透明质酸的天然牛骨替代物在眼眶保存中的组织学和组织形态学评价——附3例报告

IF 4.2 3区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of Materials Science: Materials in Medicine

Pub Date : 2025-01-07 DOI: 10.1007/s10856-024-06844-5

Damir Jelušić, Katarina Komar Milas, Marija Čandrlić, Ivana Butorac Prpić, Branko Trajkovski, Olga Cvijanović Peloza, Željka Perić Kačarević

Tooth extraction is physiologically followed by resorption of alveolar bone. Surgical method which aims to minimise this reduction in alveolar bone with a goal to provide enough bone volume for dental implant insertion is called socket preservation. The purpose of this article was to asses clinical, histomorphometric and histological results of socket preservation conducted with natural bovine bone substitute with hyaluronate. Three patients with one or more hopeless teeth in posterior region planned for extraction and implant placement were included in these case reports. After atraumatic extractions, empty sockets were filled with the bovine xenograft with hyaluronate, and then covered with collagen sponge. After 4–7.5 months the samples for biopsy were taken and then implants were inserted. The augmented sites healed uneventfully and without any complications. The histological specimens demonstrated new bone formation and osteoclastic activity around the biomaterial, as well as blood vessels in soft tissue. Histomorphometrically, formation of new bone averaged 24.8% ± 4.7% (mean ± standard deviation) in bone biopsies taken from the center of the augmented site, while the residual biomaterial averaged 52.7% ± 4.9% and the soft tissue averaged 22.6% ± 4%. In conclusion, the natural bovine bone substitute with hyaluronate demonstrated excellent osteoconductive potential for bone regeneration.

Graphical Abstract

拔牙之后是牙槽骨的生理吸收。手术方法的目的是尽量减少牙槽骨的减少，目的是提供足够的骨容量，为牙种植体插入被称为窝保存。本文的目的是评估用含透明质酸的天然牛骨替代物进行骨窝保存的临床、组织形态学和组织学结果。本文报告了三例后牙区有一颗或多颗牙无望拔牙及种植体放置的患者。无创性提取后，用透明质酸填充牛异种移植物，然后用胶原蛋白海绵覆盖。4-7.5个月后取活检标本，植入植入物。增强的部位愈合顺利，没有任何并发症。组织学标本显示新骨形成和破骨细胞活性周围的生物材料，以及血管在软组织。在组织形态学上，从增强部位中心取的骨活检中，新骨形成平均为24.8%±4.7%（平均值±标准差），残留生物材料平均为52.7%±4.9%，软组织平均为22.6%±4%。综上所述，含透明质酸的天然牛骨替代物具有良好的骨再生导骨潜能。图形抽象

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引用次数: 0

True-bone-ceramics / type I collagen scaffolds for repairing osteochondral defect 真骨陶瓷/ I型胶原蛋白支架修复骨软骨缺损

IF 4.2 3区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of Materials Science: Materials in Medicine

Pub Date : 2024-12-30 DOI: 10.1007/s10856-024-06852-5

Yuhan Jiang, Tenghai Li, Yingyue Lou, Bingzhang Liu, Yilin Liu, Tian Li, Duo Zhang

In recent years, the incidence of cartilage defects has increased dramatically, and its etiology is complex and varied. Osteochondritis dissecans (OCD), as one of the main etiologies, damages both cartilage and bone tissues and can progress to severe osteoarthritis, which has been one of the difficult problems for clinicians. The vigorous development of material science and tissue engineering provides new ideas for the treatment of OCD, in which the selection of scaffold materials is particularly important. In this study, true-bone-ceramics (TBC), which has good mechanical strength and osteoconductivity, and type I collagen (COL1), which has excellent biocompatibility, were chosen as scaffold materials to co-construct the TBC/COL1 scaffold for osteochondral repair. In order to ensure the most appropriate collagen coating concentration, three experimental groups (1, 5, 12 mg/ml) were set up. Through the physicochemical property test, biocompatibility analysis and in vivo implantation experiments of composite scaffolds, 12 mg/ml TBC/COL1 scaffolds present the best repair effect among the three groups.

Graphical Abstract

近年来，软骨缺损的发病率急剧上升，其病因复杂多样。夹层性骨软骨炎（osteondritis夹层炎，OCD）是软骨和骨组织损伤的主要病因之一，可发展为严重的骨关节炎，一直是困扰临床医生的难题之一。材料科学和组织工程的蓬勃发展为强迫症的治疗提供了新的思路，其中支架材料的选择尤为重要。本研究选择具有良好机械强度和骨导电性的真骨陶瓷（true-bone-ceramics， TBC）和具有良好生物相容性的I型胶原（type I collagen, COL1）作为支架材料，共同构建TBC/COL1骨软骨修复支架。为保证最适宜的胶原包被浓度，设1、5、12 mg/ml 3个实验组。通过复合支架的理化性能测试、生物相容性分析和体内植入实验，3组中12mg /ml TBC/COL1支架的修复效果最好。图形抽象

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