Diseases and injuries can cause significant bone loss, leading to increased medical expenses, decreased work efficiency, and a decline in quality of life. Bone tissue engineering (BTE) is gaining attention as an alternative to autologous and allogeneic transplantation due to the limited availability of donors. Biomaterials represent a promising strategy for bone regeneration, and their design should consider the three key processes in bone tissue engineering: osteogenesis, bone conduction, and bone induction. Certain bile acids (BAs) demonstrate significant antioxidant, anti-inflammatory, and immunosuppressive properties, and effectively promote bone and tissue regeneration. Additionally, the combination of BA molecule with other biological materials can help overcome problems associated with limited local bone regeneration and maintain a defined release state for a long time. Thus in this review, we focus on the role and the mechanism of bile acids in bone healing under different conditions, highlighting their unique properties and applications in gel fabrication, microencapsulation, and nanotechnology. These advancements serve as a basis for the advancement of biomaterials derived from BAs, specifically for the purpose of bone reconstruction.
{"title":"Development of biomaterials for bone tissue engineering based on bile acids","authors":"Yongjun Liu, Xiaojie Liu, Chang Liu, Wenan Zhang, Ting Shi, Guanying Liu","doi":"10.1007/s10856-024-06850-7","DOIUrl":"10.1007/s10856-024-06850-7","url":null,"abstract":"<div><p>Diseases and injuries can cause significant bone loss, leading to increased medical expenses, decreased work efficiency, and a decline in quality of life. Bone tissue engineering (BTE) is gaining attention as an alternative to autologous and allogeneic transplantation due to the limited availability of donors. Biomaterials represent a promising strategy for bone regeneration, and their design should consider the three key processes in bone tissue engineering: osteogenesis, bone conduction, and bone induction. Certain bile acids (BAs) demonstrate significant antioxidant, anti-inflammatory, and immunosuppressive properties, and effectively promote bone and tissue regeneration. Additionally, the combination of BA molecule with other biological materials can help overcome problems associated with limited local bone regeneration and maintain a defined release state for a long time. Thus in this review, we focus on the role and the mechanism of bile acids in bone healing under different conditions, highlighting their unique properties and applications in gel fabrication, microencapsulation, and nanotechnology. These advancements serve as a basis for the advancement of biomaterials derived from BAs, specifically for the purpose of bone reconstruction.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":"36 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10856-024-06850-7.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142982466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-15DOI: 10.1007/s10856-025-06858-7
Philipp-Cornelius Pott, Karolina Petsa, Christian Klose, Julian-Tobias Schleich, Neele Brümmer, Andreas Winkel, Hans Jürgen Maier, Meike Stiesch
Although implants have undergone a remarkable development over the past decades, modern implants still show complications that make the improvement of materials necessary. The presented study investigates the load-bearing capacity of an experimental dental implant made of a niobium alloy (Nb1Zr) compared to identical implants made of Ti6Al4V using chewing simulation for artificial aging. Eight implants each with an experimental design were manufactured from Nb1Zr and Ti6Al4V. A standardized abutment made of Ti6Al4V was fabricated for each implant and screwed into the implant with a screw made of the same material. A shape-identical crown for an upper first molar was fabricated for all implants using the CAD/CAM technique. All specimens were artificially aged using chewing simulation for 1 × 106 cycles and thermocycling between 5 °C and 55 °C for 4 × 103 cycles. After that, all specimens were loaded until failure. This was followed by a 3D analysis of the deformation of the samples. 100% of the samples survived the artificial aging. The Nb1Zr samples failed at 2595 ± 1069 N. In the Ti6Al4V group, failure occurred at 2958 ± 1058 N. The first deformations occurred in both groups from a load of at least 1114 N. The 3D analysis revealed deformations of 0.08 mm in the implant shoulder area of the Nb1Zr implants and of 0.04 mm in the Ti6Al4V implants. This difference was significant (p = 0.016). The investigated Nb1Zr alloy appears to be suitable for implants. The measured load-bearing capacity is significantly higher than the expected intraoral masticatory forces.
{"title":"Load-bearing capacity of an experimental dental implant made of Nb-1Zr","authors":"Philipp-Cornelius Pott, Karolina Petsa, Christian Klose, Julian-Tobias Schleich, Neele Brümmer, Andreas Winkel, Hans Jürgen Maier, Meike Stiesch","doi":"10.1007/s10856-025-06858-7","DOIUrl":"10.1007/s10856-025-06858-7","url":null,"abstract":"<div><p>Although implants have undergone a remarkable development over the past decades, modern implants still show complications that make the improvement of materials necessary. The presented study investigates the load-bearing capacity of an experimental dental implant made of a niobium alloy (Nb1Zr) compared to identical implants made of Ti6Al4V using chewing simulation for artificial aging. Eight implants each with an experimental design were manufactured from Nb1Zr and Ti6Al4V. A standardized abutment made of Ti6Al4V was fabricated for each implant and screwed into the implant with a screw made of the same material. A shape-identical crown for an upper first molar was fabricated for all implants using the CAD/CAM technique. All specimens were artificially aged using chewing simulation for 1 × 10<sup>6</sup> cycles and thermocycling between 5 °C and 55 °C for 4 × 10<sup>3</sup> cycles. After that, all specimens were loaded until failure. This was followed by a 3D analysis of the deformation of the samples. 100% of the samples survived the artificial aging. The Nb1Zr samples failed at 2595 ± 1069 N. In the Ti6Al4V group, failure occurred at 2958 ± 1058 N. The first deformations occurred in both groups from a load of at least 1114 N. The 3D analysis revealed deformations of 0.08 mm in the implant shoulder area of the Nb1Zr implants and of 0.04 mm in the Ti6Al4V implants. This difference was significant (<i>p</i> = 0.016). The investigated Nb1Zr alloy appears to be suitable for implants. The measured load-bearing capacity is significantly higher than the expected intraoral masticatory forces.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":"36 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10856-025-06858-7.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142982467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In-stent restenosis (ISR) following interventional therapy is a fatal clinical complication. Current evidence indicates that neointimal hyperplasia driven by uncontrolled proliferation of vascular smooth muscle cells (VSMC) is a major cause of restenosis. This implies that inhibiting VSMC proliferation may be an attractive approach for preventing in-stent restenosis. In our previous study, we found that the iron stent reduced the neointimal hyperplasia in an atherosclerotic artery stenosis model, and the iron corroded granules generated by the iron stent inhibited neointimal hyperplasia by suppressing the proliferation of VSMCs. However, this observation needs to be validated through in vitro experimentation. In this study, co-culture experiments and flow cytometer assays were performed to qualitatively investigate the effects of iron stent degradation on VSMCs. Moreover, the degraded products resulting generated by the iron stent were collected and used to elucidate the suppressive effect of the iron stents. The underlying mechanism was explored through molecular biology assays. The major findings are as follows: 1) The degraded iron stent inhibited the proliferation of VSMCs; 2) The degraded products of the iron stent downregulated the expression of AP-1. In summary, this study demonstrates the inhibitory effect of degraded iron products on VSMC proliferation, implying that such products have the potential to mitigate in-stent restenosis.
Graphical Abstract
degraded products generated from iron stent inhibit the vascular smooth muscle cell proliferation by downregulating AP-1.
{"title":"Degraded products generated by iron stent inhibit the vascular smooth muscle cell proliferation by downregulating AP-1","authors":"Jiabing Huang, Bingjian Liu, Chunguang Zhao, Jing Li, Dongxu Qiu","doi":"10.1007/s10856-024-06854-3","DOIUrl":"10.1007/s10856-024-06854-3","url":null,"abstract":"<div><p>In-stent restenosis (ISR) following interventional therapy is a fatal clinical complication. Current evidence indicates that neointimal hyperplasia driven by uncontrolled proliferation of vascular smooth muscle cells (VSMC) is a major cause of restenosis. This implies that inhibiting VSMC proliferation may be an attractive approach for preventing in-stent restenosis. In our previous study, we found that the iron stent reduced the neointimal hyperplasia in an atherosclerotic artery stenosis model, and the iron corroded granules generated by the iron stent inhibited neointimal hyperplasia by suppressing the proliferation of VSMCs. However, this observation needs to be validated through in vitro experimentation. In this study, co-culture experiments and flow cytometer assays were performed to qualitatively investigate the effects of iron stent degradation on VSMCs. Moreover, the degraded products resulting generated by the iron stent were collected and used to elucidate the suppressive effect of the iron stents. The underlying mechanism was explored through molecular biology assays. The major findings are as follows: 1) The degraded iron stent inhibited the proliferation of VSMCs; 2) The degraded products of the iron stent downregulated the expression of AP-1. In summary, this study demonstrates the inhibitory effect of degraded iron products on VSMC proliferation, implying that such products have the potential to mitigate in-stent restenosis.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div><div><p>degraded products generated from iron stent inhibit the vascular smooth muscle cell proliferation by downregulating AP-1.</p></div></div></figure></div></div>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":"36 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10856-024-06854-3.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142962970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-13DOI: 10.1007/s10856-024-06857-0
S. F. Mirtaheri, S. N. Mousavi, Z. Abdi, E. Hosseini, M. S. Seyed Dorraji, F. Kabiri Esfahani, M. Gholami
Preserving fertility is important in men under radiation therapy because healthy cells are also affected by radiation. Supplementation with antioxidants is a controversial issue in this process. Designing a biocompatible delivery system containing hydrophobic antioxidants to release control may solve these disagreements. Herein, a bifunctional periodic mesoporous organosilica (PMO) was designed to load quercetin (Quer) and its application was studied on damaged cells induced by irradiation on the male reproductive system. Quercetin-loaded on PMO significantly improved the length and width of the testis after irradiation compared to the Quer, alone (p < 0.001 and p < 0.001, respectively). Sperm viability was significantly higher in the Quer-loaded on PMO than Quer, alone after irradiation (p < 0.001). Irradiation significantly decreased the sperm count (p = 0.01), however, Quer and Quer-loaded on PMO could not increase them to the normal ranges. Quer alone and loaded on PMO significantly reduced the sperm with abnormal morphology after irradiation (p < 0.001). Quer alone, and loaded on PMO significantly increased daily production of sperm after irradiation (p < 0.001). The number of apoptotic cells significantly increased after irradiation (p < 0.001). After irradiation, Quer loaded on PMO significantly decreased the apoptotic cells compared to the irradiated (p < 0.001) and Quer, alone groups (p < 0.001). The novel synthesized PMO containing Quer reduced the side effects of irradiation on the male reproductive system.
{"title":"Protective effect of quercetin loaded on bifunctional periodic mesoporous organosilica against damage induced by irradiation on the male reproductive system","authors":"S. F. Mirtaheri, S. N. Mousavi, Z. Abdi, E. Hosseini, M. S. Seyed Dorraji, F. Kabiri Esfahani, M. Gholami","doi":"10.1007/s10856-024-06857-0","DOIUrl":"10.1007/s10856-024-06857-0","url":null,"abstract":"<div><p>Preserving fertility is important in men under radiation therapy because healthy cells are also affected by radiation. Supplementation with antioxidants is a controversial issue in this process. Designing a biocompatible delivery system containing hydrophobic antioxidants to release control may solve these disagreements. Herein, a bifunctional periodic mesoporous organosilica (PMO) was designed to load quercetin (Quer) and its application was studied on damaged cells induced by irradiation on the male reproductive system. Quercetin-loaded on PMO significantly improved the length and width of the testis after irradiation compared to the Quer, alone (<i>p</i> < 0.001 and <i>p</i> < 0.001, respectively). Sperm viability was significantly higher in the Quer-loaded on PMO than Quer, alone after irradiation (<i>p</i> < 0.001). Irradiation significantly decreased the sperm count (<i>p</i> = 0.01), however, Quer and Quer-loaded on PMO could not increase them to the normal ranges. Quer alone and loaded on PMO significantly reduced the sperm with abnormal morphology after irradiation (<i>p</i> < 0.001). Quer alone, and loaded on PMO significantly increased daily production of sperm after irradiation (<i>p</i> < 0.001). The number of apoptotic cells significantly increased after irradiation (<i>p</i> < 0.001). After irradiation, Quer loaded on PMO significantly decreased the apoptotic cells compared to the irradiated (<i>p</i> < 0.001) and Quer, alone groups (<i>p</i> < 0.001). The novel synthesized PMO containing Quer reduced the side effects of irradiation on the male reproductive system.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":"36 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10856-024-06857-0.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142962968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-13DOI: 10.1007/s10856-024-06848-1
Safaa Gamal, Mina Mikhail, Nancy Salem, Mohamed Tarek EL-Wakad, Reda Abdelbaset
Bone cement is commonly utilized to secure prosthetic joints in the body because of its robust fixation, stability, biocompatibility, and immediate load-bearing capability. However, issues such as loosening, leakage, and insufficient bioactivity can lead to its failure. Therefore, improving its mechanical, physical, and biological properties is crucial for enhancing its efficiency. This study examines the impact of incorporating four different nanomaterials—Titanium Dioxide (TiO2), Magnesium Oxide (MgO), Calcium Phosphate (Ca3(PO4)2), and Alumina Oxide (Al2O3)—into bone cement on its mechanical, physical, and biological properties. TiO2 and Al2O3 nanoparticles are selected to enhance the compression strength of bone cement, thereby preventing loosening. Magnesium Oxide (MgO) and Ca3(PO4)2 nanoparticles are chosen to improve cell adhesion and reducing the risk of cement leakage. Five specimens were prepared: the first with 100% pure bone cement powder, the second with 98% pure bone cement powder and modified with 2% MgO and TiO2, and the remaining three with 95% pure bone cement powder and modified with 5% varying ratios of MgO, TiO2, Ca3(PO4)2, and Al2O3. Compression, tensile, hardness, and bending strengths were assessed to determine improvements in mechanical properties. Setting temperature, porosity, and degradation were measured to evaluate physical properties. Cell adhesion and toxicity tests were conducted to examine the surface structure and biological properties. The results demonstrated that the modified specimens increased compression strength by 8.14%, tensile strength by 3.4%, and bending strength by 4.96%. Porosity, degradation, and setting temperature in modified specimens increased by 3.24%, 0.64%, and 5.17% respectively pure bone cement values. Cell adhesion in modified bone cement specimens showed normal attachment when scanned with FE-SEM. All of the tested modified specimens showed no toxicity, except for specimens with 2% Al2O3 that showed 25% toxicity which could be averted by employing antibiotics.
{"title":"Enhanced bone cement for fixation of prosthetic joint utilizing nanoparticles","authors":"Safaa Gamal, Mina Mikhail, Nancy Salem, Mohamed Tarek EL-Wakad, Reda Abdelbaset","doi":"10.1007/s10856-024-06848-1","DOIUrl":"10.1007/s10856-024-06848-1","url":null,"abstract":"<div><p>Bone cement is commonly utilized to secure prosthetic joints in the body because of its robust fixation, stability, biocompatibility, and immediate load-bearing capability. However, issues such as loosening, leakage, and insufficient bioactivity can lead to its failure. Therefore, improving its mechanical, physical, and biological properties is crucial for enhancing its efficiency. This study examines the impact of incorporating four different nanomaterials—Titanium Dioxide (TiO<sub>2</sub>), Magnesium Oxide (MgO), Calcium Phosphate (Ca<sub>3</sub>(PO<sub>4</sub>)<sub>2</sub>), and Alumina Oxide (Al<sub>2</sub>O<sub>3</sub>)—into bone cement on its mechanical, physical, and biological properties. TiO<sub>2</sub> and Al<sub>2</sub>O<sub>3</sub> nanoparticles are selected to enhance the compression strength of bone cement, thereby preventing loosening. Magnesium Oxide (MgO) and Ca<sub>3</sub>(PO<sub>4</sub>)<sub>2</sub> nanoparticles are chosen to improve cell adhesion and reducing the risk of cement leakage. Five specimens were prepared: the first with 100% pure bone cement powder, the second with 98% pure bone cement powder and modified with 2% MgO and TiO2, and the remaining three with 95% pure bone cement powder and modified with 5% varying ratios of MgO, TiO<sub>2</sub>, Ca<sub>3</sub>(PO<sub>4</sub>)<sub>2</sub>, and Al<sub>2</sub>O<sub>3</sub>. Compression, tensile, hardness, and bending strengths were assessed to determine improvements in mechanical properties. Setting temperature, porosity, and degradation were measured to evaluate physical properties. Cell adhesion and toxicity tests were conducted to examine the surface structure and biological properties. The results demonstrated that the modified specimens increased compression strength by 8.14%, tensile strength by 3.4%, and bending strength by 4.96%. Porosity, degradation, and setting temperature in modified specimens increased by 3.24%, 0.64%, and 5.17% respectively pure bone cement values. Cell adhesion in modified bone cement specimens showed normal attachment when scanned with FE-SEM. All of the tested modified specimens showed no toxicity, except for specimens with 2% Al<sub>2</sub>O<sub>3</sub> that showed 25% toxicity which could be averted by employing antibiotics.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":"36 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10856-024-06848-1.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142962969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Preservation and long-term storage of readily available cell-laden tissue-engineered products are major challenges in expanding their applications in healthcare. In recent years, there has been increasing interest in the development of off-the-shelf tissue-engineered products using the cryobioprinting approach. Here, bioinks are incorporated with cryoprotective agents (CPAs) to allow the fabrication of cryopreservable tissue constructs. Although this method has shown potential in the fabrication of cryopreservable tissue-engineered products, the impact of the CPAs on the viscoelastic behavior and printability of the bioinks at cryo conditions remains unexplored. In this study, we have evaluated the influence of CPAs such as glycerol and dimethyl sulfoxide (DMSO) on the rheological properties of pre-crosslinked alginate bioinks for cryoprinting applications. DMSO-incorporated bioinks showed a reduction in viscosity and yield stress, while the addition of glycerol improved both the properties due to interactions with the calcium chloride used for pre-crosslinking. Further, tube inversion and printability experiments were performed to identify suitable concentrations and cryobioprinting conditions for bioinks containing CPAs & pre-crosslinked with CaCl2. Finally, based on the printability analysis & cell recovery results, 10% glycerol was used for cryobioprinting and preservation of cell-laden constructs at −80 °C and the viability of cells within the printed structures were evaluated after recovery. Cell viability results indicate that the addition of 10% glycerol to the pre-crosslinked bioink significantly improved cell viability compared to bioinks without CPAs, confirming the suitability of the developed bioink combination to fabricate tissue constructs for on-demand applications.
Graphical abstract
Effect of cryoprotectants on the viscoelastic behavior of bioinks and cell recovery in cryobioprinted tissue constructs.
{"title":"Insights on the role of cryoprotectants in enhancing the properties of bioinks required for cryobioprinting of biological constructs","authors":"Harshavardhan Budharaju, Dhakshinamoorthy Sundaramurthi, Swaminathan Sethuraman","doi":"10.1007/s10856-024-06855-2","DOIUrl":"10.1007/s10856-024-06855-2","url":null,"abstract":"<div><p>Preservation and long-term storage of readily available cell-laden tissue-engineered products are major challenges in expanding their applications in healthcare. In recent years, there has been increasing interest in the development of off-the-shelf tissue-engineered products using the cryobioprinting approach. Here, bioinks are incorporated with cryoprotective agents (CPAs) to allow the fabrication of cryopreservable tissue constructs. Although this method has shown potential in the fabrication of cryopreservable tissue-engineered products, the impact of the CPAs on the viscoelastic behavior and printability of the bioinks at cryo conditions remains unexplored. In this study, we have evaluated the influence of CPAs such as glycerol and dimethyl sulfoxide (DMSO) on the rheological properties of pre-crosslinked alginate bioinks for cryoprinting applications. DMSO-incorporated bioinks showed a reduction in viscosity and yield stress, while the addition of glycerol improved both the properties due to interactions with the calcium chloride used for pre-crosslinking. Further, tube inversion and printability experiments were performed to identify suitable concentrations and cryobioprinting conditions for bioinks containing CPAs & pre-crosslinked with CaCl<sub>2</sub>. Finally, based on the printability analysis & cell recovery results, 10% glycerol was used for cryobioprinting and preservation of cell-laden constructs at −80 °C and the viability of cells within the printed structures were evaluated after recovery. Cell viability results indicate that the addition of 10% glycerol to the pre-crosslinked bioink significantly improved cell viability compared to bioinks without CPAs, confirming the suitability of the developed bioink combination to fabricate tissue constructs for on-demand applications.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div><div><p>Effect of cryoprotectants on the viscoelastic behavior of bioinks and cell recovery in cryobioprinted tissue constructs.</p></div></div></figure></div></div>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":"36 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10856-024-06855-2.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142963069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-07DOI: 10.1007/s10856-024-06847-2
Mohammad Amiri, Zahra Hashemi, Fereshteh Chekin
The antimalarial hydroxychloroquine (HCQ) has considered for the treatment of systemic lupus erythematosus. Moreover, HCQ has been used as a drug to treat Coronavirus disease (COVID-19). In this work, nitrogen doped porous reduced graphene oxide (NprGO) has been prepared via environmentally friendly process using Fummaria Parviflora extract. A catalyst based on ZnO nanoparticles-nitrogen doped porous reduced graphene oxide (ZnO-NprGO) was prepared by hydrothermal method and characterized. The diameter of ZnO nanoparticles was ~22–37 nm, which were inserted between the NprGO sheets effectively prevented their aggregation. The ZnO-NprGO hybrid had high surface area and good electro-catalytic property, suiting for determination of HCQ. The ZnO-NprGO modified carbon paste electrode (CPE)-based sensor operated in a wide concentration range of 0.07–5.5 μmol L−1 with low limit of detection of 57 nmol L−1 and sensitivity of 14.175 μA μmol−1 L. Remarkably, the ZnO-NprGO/CPE sensor indicated acceptable accuracy, reproducibility, and stability. In addition, the proposed sensor was applied to detection of HCQ in biological samples and the recoveries were 92.0–102.5%, with relative standard deviations of 1.9–4.3%. The unique physical structure of ZnO-NprGO, as well as its chemical and electrical properties, make it promising interface for use in sensors and nanoelectronic applications.
{"title":"Zinc oxide nanoparticles decorated nitrogen doped porous reduced graphene oxide-based hybrid to sensitive detection of hydroxychloroquine in plasma and urine","authors":"Mohammad Amiri, Zahra Hashemi, Fereshteh Chekin","doi":"10.1007/s10856-024-06847-2","DOIUrl":"10.1007/s10856-024-06847-2","url":null,"abstract":"<div><p>The antimalarial hydroxychloroquine (HCQ) has considered for the treatment of systemic lupus erythematosus. Moreover, HCQ has been used as a drug to treat Coronavirus disease (COVID-19). In this work, nitrogen doped porous reduced graphene oxide (NprGO) has been prepared via environmentally friendly process using Fummaria Parviflora extract. A catalyst based on ZnO nanoparticles-nitrogen doped porous reduced graphene oxide (ZnO-NprGO) was prepared by hydrothermal method and characterized. The diameter of ZnO nanoparticles was ~22–37 nm, which were inserted between the NprGO sheets effectively prevented their aggregation. The ZnO-NprGO hybrid had high surface area and good electro-catalytic property, suiting for determination of HCQ. The ZnO-NprGO modified carbon paste electrode (CPE)-based sensor operated in a wide concentration range of 0.07–5.5 μmol L<sup>−1</sup> with low limit of detection of 57 nmol L<sup>−1</sup> and sensitivity of 14.175 μA μmol<sup>−1</sup> L. Remarkably, the ZnO-NprGO/CPE sensor indicated acceptable accuracy, reproducibility, and stability. In addition, the proposed sensor was applied to detection of HCQ in biological samples and the recoveries were 92.0–102.5%, with relative standard deviations of 1.9–4.3%. The unique physical structure of ZnO-NprGO, as well as its chemical and electrical properties, make it promising interface for use in sensors and nanoelectronic applications.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":"36 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10856-024-06847-2.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142938894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Osseointegration is essential for successful implant treatment. However, the underlying molecular mechanisms remain unclear. In this study, we focused on decorin (DCN), which was hypothesized to be present in the proteoglycan (PG) layer at the interface between bone and the titanium oxide (TiOx) surface. We utilized DCN RNA interference in human bone marrow mesenchymal stem cells (hBMSCs) to investigate its effects on PG layer formation, proliferation, initial adhesion, cell extension, osteogenic capacity, fibrotic markers, and immunotolerance to TiOx in vitro. After 14 days of cultivation, we observed no PG layer was detected, and the osteogenic capacity was suppressed in DCN-depleted hBMSCs. Furthermore, the conditioned medium upregulated the expression of M1 macrophage markers in human macrophages. These results suggest that endogenous DCN plays a crucial role in PG layer formation and that the PG layer alters inflammation around Ti materials.
{"title":"Knockdown of decorin in human bone marrow mesenchymal stem cells suppresses proteoglycan layer formation and establishes a pro-inflammatory environment on titanium oxide surfaces","authors":"Hisanobu Kamio, Kazuto Okabe, Masaki Honda, Kensuke Kuroda, Shuhei Tsuchiya","doi":"10.1007/s10856-024-06849-0","DOIUrl":"10.1007/s10856-024-06849-0","url":null,"abstract":"<div><p>Osseointegration is essential for successful implant treatment. However, the underlying molecular mechanisms remain unclear. In this study, we focused on decorin (DCN), which was hypothesized to be present in the proteoglycan (PG) layer at the interface between bone and the titanium oxide (TiOx) surface. We utilized DCN RNA interference in human bone marrow mesenchymal stem cells (hBMSCs) to investigate its effects on PG layer formation, proliferation, initial adhesion, cell extension, osteogenic capacity, fibrotic markers, and immunotolerance to TiOx in vitro. After 14 days of cultivation, we observed no PG layer was detected, and the osteogenic capacity was suppressed in DCN-depleted hBMSCs. Furthermore, the conditioned medium upregulated the expression of M1 macrophage markers in human macrophages. These results suggest that endogenous DCN plays a crucial role in PG layer formation and that the PG layer alters inflammation around Ti materials.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":"36 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10856-024-06849-0.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142938956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-07DOI: 10.1007/s10856-024-06856-1
Yadi Sun, Yan Wang, Benchao Dong, Peichuan Yang, Chunhui Ji, Yiyang Li, Jianxiong Ma, Xinlong Ma
The number of patients with bone defects caused by trauma and diseases has been increasing year by year. The treatment of bone defects remains a major challenge in clinical practice. Bone scaffolds are increasingly favored for repairing bones, with triply periodic minimal surface (TPMS) scaffolds emerging as a popular option due to their superior performance. The aim of this review is to highlight the crucial influence of pore structure on the properties of TPMS bone scaffolds, offering important insights for their innovation and production. It briefly examines various elements that influence the properties of TPMS bone scaffolds, such as pore shape, porosity, pore diameter, and curvature. By analyzing these elements, this review serves as a valuable reference for upcoming research and practical implementations in the field of bone tissue engineering.
{"title":"Understanding the relationship between pore structure and properties of triply periodic minimal surface bone scaffolds","authors":"Yadi Sun, Yan Wang, Benchao Dong, Peichuan Yang, Chunhui Ji, Yiyang Li, Jianxiong Ma, Xinlong Ma","doi":"10.1007/s10856-024-06856-1","DOIUrl":"10.1007/s10856-024-06856-1","url":null,"abstract":"<div><p>The number of patients with bone defects caused by trauma and diseases has been increasing year by year. The treatment of bone defects remains a major challenge in clinical practice. Bone scaffolds are increasingly favored for repairing bones, with triply periodic minimal surface (TPMS) scaffolds emerging as a popular option due to their superior performance. The aim of this review is to highlight the crucial influence of pore structure on the properties of TPMS bone scaffolds, offering important insights for their innovation and production. It briefly examines various elements that influence the properties of TPMS bone scaffolds, such as pore shape, porosity, pore diameter, and curvature. By analyzing these elements, this review serves as a valuable reference for upcoming research and practical implementations in the field of bone tissue engineering.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":"36 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10856-024-06856-1.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142938895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-07DOI: 10.1007/s10856-024-06836-5
Suhair Hikmat, Ola Tarawneh, Lama Hamadneh, Rania Hamed, Ala A. Alhusban, Mohammad Hailat, Hadeel Abu Mahfouz, Sawsan Shraim, Alghadeer Al-Shammari, Aya Aljariri, Rafa Abu Rayya, Lana Hamdan
Wound dressing development is an area of active research. Traditional dressings lack antibacterial activity, biocompatibility, and tissue regeneration. Alginate is a heavily investigated polymer employed as wound dressings and can be combined with a wide range of additives. Herein, we report the preparation of alginate gel using the crosslinking technique as potential wound dressing, with insight investigation of the influence of employing single, two, or three cross-linkers: Strontium (Sr), zinc oxide (ZnO), and gentamicin sulfate. Rheology was used to confirm the gel’s preparation, where the samples’ viscosity curves show decreased viscosity with increased shear rate, indicating pseudoplastic flow. The linear viscoelastic region shows constant G’ and G” within the sample structure. In this study, we used three gels with different mixtures of ingredients: Gels A, B, and C contain sodium alginate (1% w/v) and 0.5 mL of Sr nitrate (4% w/v). However, Gels B and C contain 0.25 mL of ZnO (0.5% w/v). Gel C also includes 0.1 mL of gentamicin (1% w/v). The study examined the effectiveness of Gel A, B, and C on wound healing, calculating the reduction of wound area after seven, 14, and 20 days of a single topical treatment. Gel A, B, and C significantly reduced wound area, while Gel B and C showed a significant reduction. The zone of inhibition was used to detect the gels’ efficacy against microorganisms. The study found zinc deposition in the liver and bone, with Gel B and C showing higher levels. The study also found significant overexpression of MIP α and MIP β in tissues and downregulation of CCL2, IL8, and TGF β, explaining wound healing with minimal scar formation.
{"title":"Strontium nitrate-dopped zinc oxide-loaded alginate gels with gentamicin for improved wound healing","authors":"Suhair Hikmat, Ola Tarawneh, Lama Hamadneh, Rania Hamed, Ala A. Alhusban, Mohammad Hailat, Hadeel Abu Mahfouz, Sawsan Shraim, Alghadeer Al-Shammari, Aya Aljariri, Rafa Abu Rayya, Lana Hamdan","doi":"10.1007/s10856-024-06836-5","DOIUrl":"10.1007/s10856-024-06836-5","url":null,"abstract":"<div><p>Wound dressing development is an area of active research. Traditional dressings lack antibacterial activity, biocompatibility, and tissue regeneration. Alginate is a heavily investigated polymer employed as wound dressings and can be combined with a wide range of additives. Herein, we report the preparation of alginate gel using the crosslinking technique as potential wound dressing, with insight investigation of the influence of employing single, two, or three cross-linkers: Strontium (Sr), zinc oxide (ZnO), and gentamicin sulfate. Rheology was used to confirm the gel’s preparation, where the samples’ viscosity curves show decreased viscosity with increased shear rate, indicating pseudoplastic flow. The linear viscoelastic region shows constant <i>G’</i> and <i>G”</i> within the sample structure. In this study, we used three gels with different mixtures of ingredients: Gels A, B, and C contain sodium alginate (1% w/v) and 0.5 mL of Sr nitrate (4% w/v). However, Gels B and C contain 0.25 mL of ZnO (0.5% w/v). Gel C also includes 0.1 mL of gentamicin (1% w/v). The study examined the effectiveness of Gel A, B, and C on wound healing, calculating the reduction of wound area after seven, 14, and 20 days of a single topical treatment. Gel A, B, and C significantly reduced wound area, while Gel B and C showed a significant reduction. The zone of inhibition was used to detect the gels’ efficacy against microorganisms. The study found zinc deposition in the liver and bone, with Gel B and C showing higher levels. The study also found significant overexpression of MIP α and MIP β in tissues and downregulation of CCL2, IL8, and TGF β, explaining wound healing with minimal scar formation.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":"36 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10856-024-06836-5.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142938817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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