Pub Date : 2024-12-18DOI: 10.1007/s00604-024-06857-5
Doddanagowada S, Venkata Narayana Palakollu, S. V. Prabhakar Vattikuti, Jaesool Shim, Naresh Mameda
Aflatoxins (AFs), produced by fungi, are highly hazardous and classified as mycotoxins. Controlling their levels is of significant concern. This group consists of 20 fungal metabolites, all structurally derived from difuranocoumarin. Exposure to AFs through food can cause critical health issues, such as cancers, deformities, and mutations, posing a significant global public health issue. The inherent dangers of AF exposure necessitate swift and reliable detection techniques to identify its presence in food products. The rise of nanotechnology has opened doors to innovative electrochemical biosensors, offering a promising solution to this pressing issue. This review delves into nanomaterial-based aptasensors, immunosensors, and molecularly imprinted polymers, the predominant electrochemical biosensors developed for AF detection. This paper offers a broad summary of recent advancements in biosensor technology in electrochemical sensing of AFs, alongside challenges to overcome limitations, and future perspectives.
Graphical Abstract
{"title":"Recent progress, challenges, and future perspectives of electrochemical biosensing of aflatoxins","authors":"Doddanagowada S, Venkata Narayana Palakollu, S. V. Prabhakar Vattikuti, Jaesool Shim, Naresh Mameda","doi":"10.1007/s00604-024-06857-5","DOIUrl":"10.1007/s00604-024-06857-5","url":null,"abstract":"<div><p>Aflatoxins (AFs), produced by fungi, are highly hazardous and classified as mycotoxins. Controlling their levels is of significant concern. This group consists of 20 fungal metabolites, all structurally derived from difuranocoumarin. Exposure to AFs through food can cause critical health issues, such as cancers, deformities, and mutations, posing a significant global public health issue. The inherent dangers of AF exposure necessitate swift and reliable detection techniques to identify its presence in food products. The rise of nanotechnology has opened doors to innovative electrochemical biosensors, offering a promising solution to this pressing issue. This review delves into nanomaterial-based aptasensors, immunosensors, and molecularly imprinted polymers, the predominant electrochemical biosensors developed for AF detection. This paper offers a broad summary of recent advancements in biosensor technology in electrochemical sensing of AFs, alongside challenges to overcome limitations, and future perspectives.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 1","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142844930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-16DOI: 10.1007/s00604-024-06817-z
Li Mingyao, Li Ruiyi, Li Zaijun
A biosensor is reported for electrochemical detection of ampicillin based on Au5Pt and DNA cycle dual-signal amplification strategy. Firstly, Au5Pt is prepared by reduction of chloroauric acid and chloroplatinic acid with serine-functionalized graphene quantum dot (SGQD). The resulting Au5Pt shows an excellent catalytic activity because of the synergy of Au and Pt. Then, Au5Pt is covalently combined with hairpin DNA and thionine molecule to form a redox probe. The probe was used for construction of the ampicillin biosensor coupling with DNA cycling. In the presence of ampicillin, the DNA cycle was triggered and leads to many redox probes being carried to the biosensor. This produces a significant signal amplification by oxidation and reduction of thionine molecules in these probes. The combination of Au5Pt catalysis with DNA cycle achieve ultrahigh sensitivity, selectivity, and stability for the electrochemical detection of ampicillin. Differential pulse voltammetry current linearly increases with the increase of ampicillin concentration in the range 1 × 10−18–1 × 10−12 M with a detection limit of 3.2 × 10−19 M (S/N = 3). The proposed analytical method has been satisfactorily used for electrochemical detection of ampicillin in milk.
Graphical Abstract
报告了一种基于 Au5Pt 和 DNA 循环双信号放大策略的氨苄西林电化学检测生物传感器。首先，用丝氨酸功能化石墨烯量子点（SGQD）还原氯金酸和氯铂酸制备 Au5Pt。由于 Au 和 Pt 的协同作用，制备出的 Au5Pt 具有极佳的催化活性。然后，Au5Pt 与发夹 DNA 和亚硫氨酸分子共价结合，形成氧化还原探针。该探针用于构建与 DNA 循环耦合的氨苄西林生物传感器。在氨苄西林存在的情况下，DNA 循环被触发，导致许多氧化还原探针被带到生物传感器。通过氧化和还原这些探针中的亚硫氨酸分子，产生了显著的信号放大效果。Au5Pt 催化与 DNA 循环的结合实现了氨苄西林电化学检测的超高灵敏度、选择性和稳定性。在 1 × 10-18-1 × 10-12 M 的范围内，差分脉冲伏安电流随氨苄西林浓度的增加而线性增加，检测限为 3.2 × 10-19 M（信噪比为 3）。所提出的分析方法可用于牛奶中氨苄西林的电化学检测。
{"title":"Electrochemical biosensor for detection of ampicillin in milk based on Au5Pt and DNA cycle dual-signal amplification strategy","authors":"Li Mingyao, Li Ruiyi, Li Zaijun","doi":"10.1007/s00604-024-06817-z","DOIUrl":"10.1007/s00604-024-06817-z","url":null,"abstract":"<div><p>A biosensor is reported for electrochemical detection of ampicillin based on Au<sub>5</sub>Pt and DNA cycle dual-signal amplification strategy. Firstly, Au<sub>5</sub>Pt is prepared by reduction of chloroauric acid and chloroplatinic acid with serine-functionalized graphene quantum dot (SGQD). The resulting Au<sub>5</sub>Pt shows an excellent catalytic activity because of the synergy of Au and Pt. Then, Au<sub>5</sub>Pt is covalently combined with hairpin DNA and thionine molecule to form a redox probe. The probe was used for construction of the ampicillin biosensor coupling with DNA cycling. In the presence of ampicillin, the DNA cycle was triggered and leads to many redox probes being carried to the biosensor. This produces a significant signal amplification by oxidation and reduction of thionine molecules in these probes. The combination of Au<sub>5</sub>Pt catalysis with DNA cycle achieve ultrahigh sensitivity, selectivity, and stability for the electrochemical detection of ampicillin. Differential pulse voltammetry current linearly increases with the increase of ampicillin concentration in the range 1 × 10<sup>−18</sup>–1 × 10<sup>−12</sup> M with a detection limit of 3.2 × 10<sup>−19</sup> M (<i>S</i>/<i>N</i> = 3). The proposed analytical method has been satisfactorily used for electrochemical detection of ampicillin in milk.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 1","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142826354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-16DOI: 10.1007/s00604-024-06853-9
Yao Yang, Zini Zhang, Zhi Wang, Ruxin Pan, Huimin Wu, Shanshan Zhai, Gang Wu, Wei Fu, Hongfei Gao
A multi-chromatic and multi-component lateral flow immunoassay (MCMC-LFIA) was developed for simultaneous detection of CP4 EPSPS, Bt-Cry1Ab, Bt-Cry1Ac, and PAT/bar proteins in genetically modified (GM) crops. Captured antibodies specific to these exogenous proteins were separately immobilized on a nitrocellulose membrane as test zones. Multi-colored microspheres, used as visible multi-probes, were conjugated with corresponding antibodies and sprayed on the conjugate pad. The assay results can be visually interpreted within 10 min by observing the appearance of colored bands. The MCMC-LFIA demonstrated high sensitivity, with detection of limits of 7.8 ng/mL for CP4 EPSPS and 2.5 ng/mL for Bt-Cry1Ab, Bt-Cry1Ac, and PAT/bar proteins, significantly improving the performance of previously reported LFIAs. The MCMC-LFIA exhibited excellent specificity and was validated for practical use in field-based applications. The proposed MCMC-LFIA offers a rapid, sensitive, and user-friendly tool for the on-site large-scale screening of GM materials.
{"title":"Multi-chromatic and multi-component lateral flow immunoassay for simultaneous detection of CP4 EPSPS, Bt-Cry1Ab, Bt-Cry1Ac, and PAT/bar proteins in genetically modified crops","authors":"Yao Yang, Zini Zhang, Zhi Wang, Ruxin Pan, Huimin Wu, Shanshan Zhai, Gang Wu, Wei Fu, Hongfei Gao","doi":"10.1007/s00604-024-06853-9","DOIUrl":"10.1007/s00604-024-06853-9","url":null,"abstract":"<div><p>A multi-chromatic and multi-component lateral flow immunoassay (MCMC-LFIA) was developed for simultaneous detection of CP4 EPSPS, Bt-Cry1Ab, Bt-Cry1Ac, and PAT/<i>bar</i> proteins in genetically modified (GM) crops. Captured antibodies specific to these exogenous proteins were separately immobilized on a nitrocellulose membrane as test zones. Multi-colored microspheres, used as visible multi-probes, were conjugated with corresponding antibodies and sprayed on the conjugate pad. The assay results can be visually interpreted within 10 min by observing the appearance of colored bands. The MCMC-LFIA demonstrated high sensitivity, with detection of limits of 7.8 ng/mL for CP4 EPSPS and 2.5 ng/mL for Bt-Cry1Ab, Bt-Cry1Ac, and PAT/<i>bar</i> proteins, significantly improving the performance of previously reported LFIAs. The MCMC-LFIA exhibited excellent specificity and was validated for practical use in field-based applications. The proposed MCMC-LFIA offers a rapid, sensitive, and user-friendly tool for the on-site large-scale screening of GM materials.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 1","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142826353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A novel molecular-imprinted electrochemical sensor for 3,4-benzopyrene (3, 4-BaP) in food samples, with high sensitivity and selective detection, is introduced. Firstly, graphene oxide was modified onto a glassy carbon electrode (GCE) by electroreduction deposition to form an RGO/GCE sensing platform, thereby enhancing the sensitivity and stability of the sensor. Then, magnetic molecularly imprinted polymer-zeolite imidazole framework-8 (MMIP-ZIF-8) was synthesized in one step using the crystal growth method and modified onto RGO/GCE, endowing the sensor with good adsorption capacity and selectivity. The performance of the sensor for 3, 4-BaP was studied using differential pulse voltammetry (DPV), and the detection conditions of the constructed sensor were optimized. The results showed that under the optimal conditions, the constructed sensor exhibited a wide linear range (0.5 ~ 1000 nmol L−1), a low limit of detection (0.16 nmol L−1), and good selectivity and stability for the detection of 3, 4-BaP. It also showed a good recovery (99.74 ~ 102.58%) for the detection of 3, 4-BaP in actual corn meal samples. The MMIP-ZIF-8/RGO/GCE sensor developed has potential application prospects in the precise detection of 3, 4-BaP in food.
Graphical Abstract
{"title":"Construction of an electrochemical sensor based on magnetic molecularly imprinted polymer-zeolite imidazole framework-8 for detection of 3,4-benzopyrene","authors":"Binbin Zhou, Xinyi Li, Hao Xie, Xingxin Sheng, Lijun Huang, Yongbo Zhu, Meng Liang, Ming Zhong","doi":"10.1007/s00604-024-06858-4","DOIUrl":"10.1007/s00604-024-06858-4","url":null,"abstract":"<div><p> A novel molecular-imprinted electrochemical sensor for 3,4-benzopyrene (3, 4-BaP) in food samples, with high sensitivity and selective detection, is introduced. Firstly, graphene oxide was modified onto a glassy carbon electrode (GCE) by electroreduction deposition to form an RGO/GCE sensing platform, thereby enhancing the sensitivity and stability of the sensor. Then, magnetic molecularly imprinted polymer-zeolite imidazole framework-8 (MMIP-ZIF-8) was synthesized in one step using the crystal growth method and modified onto RGO/GCE, endowing the sensor with good adsorption capacity and selectivity. The performance of the sensor for 3, 4-BaP was studied using differential pulse voltammetry (DPV), and the detection conditions of the constructed sensor were optimized. The results showed that under the optimal conditions, the constructed sensor exhibited a wide linear range (0.5 ~ 1000 nmol L<sup>−1</sup>), a low limit of detection (0.16 nmol L<sup>−1</sup>), and good selectivity and stability for the detection of 3, 4-BaP. It also showed a good recovery (99.74 ~ 102.58%) for the detection of 3, 4-BaP in actual corn meal samples. The MMIP-ZIF-8/RGO/GCE sensor developed has potential application prospects in the precise detection of 3, 4-BaP in food.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 1","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142810921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Multi-wavelength emission fluorescent manganese-nitrogen co-doped carbon dots (Mn, N co-doped CDs) were synthesized by solvothermal method using β-cyclodextrin, O-phenylenediamine, and manganese chloride as raw materials. The prepared Mn, N co-doped CDs were used as fluorescent nanosensing platforms for the detection of metal ions and biomolecules and were found to be capable of fluorescence detection of tannic acid (TA) and hafnium (Hf) ion at 320, 380, and 480 nm excitation wavelengths with multi-response linear ranges of 0.7 ~ 1.2 µM and 6.35 ~ 13 µM and detection limits of 0.45 µM and 6.3 µM, respectively. The wide linear ranges and low detection limits may be due to the fluorescence resonance energy transfer effect between the platform and TA and Hf ions. In addition, it was found that Mn, N co-doped CDs had good photostability, biocompatibility, and low cytotoxicity, which could be used for in vitro fluorescence imaging of exogenous TA and Hf ion imaging in U2OS osteosarcoma cells. Thus, the probe has a promising application in biomedical fields as a new multi-responsive fluorescence nanosensing platform member.
Graphical abstract
{"title":"Mn, N co-doped CDs as a fluorescent nanosensing platform for the detection of tannic acid and hafnium ion and in vitro fluorescence imaging of U2OS osteosarcoma cells","authors":"Xue-Lin Zhao, Sen-Zhen Wang, Lihua Zhang, Zhen Wang, Jin-Yan Huang, Song Liao, Min Lu, Zhi Yang, Xing-Jun Zhao, Zi-Yi Zhao, Zi-Xuan Guo, Lu-Nan Zhang, Pei-De Zhu, Meng Xu","doi":"10.1007/s00604-024-06848-6","DOIUrl":"10.1007/s00604-024-06848-6","url":null,"abstract":"<div><p>Multi-wavelength emission fluorescent manganese-nitrogen co-doped carbon dots (Mn, N co-doped CDs) were synthesized by solvothermal method using <i>β</i>-cyclodextrin, O-phenylenediamine, and manganese chloride as raw materials. The prepared Mn, N co-doped CDs were used as fluorescent nanosensing platforms for the detection of metal ions and biomolecules and were found to be capable of fluorescence detection of tannic acid (TA) and hafnium (Hf) ion at 320, 380, and 480 nm excitation wavelengths with multi-response linear ranges of 0.7 ~ 1.2 µM and 6.35 ~ 13 µM and detection limits of 0.45 µM and 6.3 µM, respectively. The wide linear ranges and low detection limits may be due to the fluorescence resonance energy transfer effect between the platform and TA and Hf ions. In addition, it was found that Mn, N co-doped CDs had good photostability, biocompatibility, and low cytotoxicity, which could be used for in vitro fluorescence imaging of exogenous TA and Hf ion imaging in U2OS osteosarcoma cells. Thus, the probe has a promising application in biomedical fields as a new multi-responsive fluorescence nanosensing platform member.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 1","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00604-024-06848-6.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142798340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A novel nanobiosensor was constructed by in situ locating nanometer MnO2 particles with controllable size and morphology in a Zr-MOF substrate to serve as an electrochemical probe. The synergistic effect of the two components, Zr-MOFs with high specific surface area and compatibility as a carrier for MnO2, resulted in improved electrochemical activity and excellent electrochemical identification performance for the MnO2@Zr-MOF/GCE biosensor. Under optimized experimental conditions and using CV and DPV technology, the biosensor showed a wide linear detection range (2–200 μM), a low detection limit (2.577 × 10−8 M), a recovery range (106.26–115.01%), and maximum relative standard deviation (5.155) for tetracycline (TC) identification. The recognition mechanism of the sensor was investigated adopting Laviron adsorption theory. The applicability of the sensor was verified through practical measurements. Overall, the MnO2 @Zr-MOF/GCE sensor possesses the advantages of fast analysis speed, high sensitivity, high selectivity, and simple operation, making it suitable for detecting trace amounts of TC in food.
Graphical abstract
{"title":"MnO2 nanoparticles enhance the activity of the Zr-MOF matrix electrochemical sensor for efficiently identifying ultra-trace tetracycline residues in food","authors":"Siyu Tian, Jiahui Wang, Yu Jie, Zhu Ding, Xiao Wang, Jijiang Wang, Xiangyang Hou","doi":"10.1007/s00604-024-06854-8","DOIUrl":"10.1007/s00604-024-06854-8","url":null,"abstract":"<div><p> A novel nanobiosensor was constructed by in situ locating nanometer MnO<sub>2</sub> particles with controllable size and morphology in a Zr-MOF substrate to serve as an electrochemical probe. The synergistic effect of the two components, Zr-MOFs with high specific surface area and compatibility as a carrier for MnO<sub>2</sub>, resulted in improved electrochemical activity and excellent electrochemical identification performance for the MnO<sub>2</sub>@Zr-MOF/GCE biosensor. Under optimized experimental conditions and using CV and DPV technology, the biosensor showed a wide linear detection range (2–200 μM), a low detection limit (2.577 × 10<sup>−8</sup> M), a recovery range (106.26–115.01%), and maximum relative standard deviation (5.155) for tetracycline (TC) identification. The recognition mechanism of the sensor was investigated adopting Laviron adsorption theory. The applicability of the sensor was verified through practical measurements. Overall, the MnO<sub>2</sub> @Zr-MOF/GCE sensor possesses the advantages of fast analysis speed, high sensitivity, high selectivity, and simple operation, making it suitable for detecting trace amounts of TC in food.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 1","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142790426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-06DOI: 10.1007/s00604-024-06865-5
Yongkai Lu, Jiaxin Xu, Siyan Liu, Baochun Tian, Feng Long
A novel hybridization chain reaction (HCR) powered optical fiber-embedded microfluidic biochip (HCR-FMB) has been constructed for ultrafast and sensitive detection of lethal-7a (let-7a) in serum. By integrating HCR, fluorescence energy resonant transfer, and evanescent wave fluorescence principle, the HCR-FMB enables detecting let-7a with satisfactory limit of detection of 100.0 pM within 6 min at room temperature, and demonstrates excellent specificity. The HCR-FMB can directly detect let-7a in serum with high sensitivity and without any pre-treatment, and good recoveries were observed for let-7a in serum samples, demonstrating their potential application to the analysis of serum samples. The HCR-FMB exhibits several advantages, including rapidity, enzyme-free, miniaturization, ease-of-operation, field-deployment applicability, minimal-equipment, and cost-effectiveness. The HCR-FMB can be considered a revolutionary detection device that rapidly adapts and deploys in various settings, especially in low medical resource regions.
Graphical Abstract
{"title":"A facile optical fiber-embedded microfluidic biochip for rapid and sensitive detection of microRNA-let-7a in serum","authors":"Yongkai Lu, Jiaxin Xu, Siyan Liu, Baochun Tian, Feng Long","doi":"10.1007/s00604-024-06865-5","DOIUrl":"10.1007/s00604-024-06865-5","url":null,"abstract":"<div><p> A novel hybridization chain reaction (HCR) powered optical fiber-embedded microfluidic biochip (HCR-FMB) has been constructed for ultrafast and sensitive detection of lethal-7a (let-7a) in serum. By integrating HCR, fluorescence energy resonant transfer, and evanescent wave fluorescence principle, the HCR-FMB enables detecting let-7a with satisfactory limit of detection of 100.0 pM within 6 min at room temperature, and demonstrates excellent specificity. The HCR-FMB can directly detect let-7a in serum with high sensitivity and without any pre-treatment, and good recoveries were observed for let-7a in serum samples, demonstrating their potential application to the analysis of serum samples. The HCR-FMB exhibits several advantages, including rapidity, enzyme-free, miniaturization, ease-of-operation, field-deployment applicability, minimal-equipment, and cost-effectiveness. The HCR-FMB can be considered a revolutionary detection device that rapidly adapts and deploys in various settings, especially in low medical resource regions.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 1","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142778587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A dual-mode immunoassay method was developed for colorimetric and fluorescence detection of aflatoxin B1 (AFB1) based on streptavidin-induced gold nanoparticle aggregation (AuNP@SA). AuNP-modified streptavidin–biotin labeling AFB1 complete antigen aggregations (AuNP@SA@Bio-BSA-AFB1) were synthesized as the competitive binding and dual-mode probe. AuNP@SA@Bio-BSA-AFB1 aggregations possessed high colorimetric and fluorescence quenching intensities. AFB1 antibodies modified immunomagnetic microspheres were used as the capture probe. The competitive binding between AFB1 and AuNP@SA@Bio-BSA-AFB1 leads to changes in color and fluorescence intensity. The detection limit of the colorimetric method is 6.95 ng·mL−1, while that of the fluorescence method is 0.07 ng·mL−1. The practicality of the proposed strategy was demonstrated by determining AFB1 in spiked peanut samples.
Graphical Abstract
{"title":"Development of a colorimetric/fluorescence dual-mode immunoassay for aflatoxin B1 based on streptavidin-induced gold nanoparticle aggregation","authors":"Yaqian Cheng, Chenxi Wang, Xueyu Chang, Xuexia Jia, Zesheng Liu, Baolin Liu, Zhixian Gao, Huanying Zhou","doi":"10.1007/s00604-024-06843-x","DOIUrl":"10.1007/s00604-024-06843-x","url":null,"abstract":"<div><p>A dual-mode immunoassay method was developed for colorimetric and fluorescence detection of aflatoxin B1 (AFB1) based on streptavidin-induced gold nanoparticle aggregation (AuNP@SA). AuNP-modified streptavidin–biotin labeling AFB1 complete antigen aggregations (AuNP@SA@Bio-BSA-AFB1) were synthesized as the competitive binding and dual-mode probe. AuNP@SA@Bio-BSA-AFB1 aggregations possessed high colorimetric and fluorescence quenching intensities. AFB1 antibodies modified immunomagnetic microspheres were used as the capture probe. The competitive binding between AFB1 and AuNP@SA@Bio-BSA-AFB1 leads to changes in color and fluorescence intensity. The detection limit of the colorimetric method is 6.95 ng·mL<sup>−1</sup>, while that of the fluorescence method is 0.07 ng·mL<sup>−1</sup>. The practicality of the proposed strategy was demonstrated by determining AFB1 in spiked peanut samples.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 1","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142789208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A new fluorinated imprinted adsorbent (MIA) for magnetic solid phase extraction (MSPE) of liquid crystal monomer (LCM) pollutants was one-pot prepared within 3.5 h using 4-[difluoro(3,4,5-trifluorophenoxy)methyl]-3,5-difluoro-4′-ethyl-biphenyl (DFBP) as template and 1H,1H,2H,2H-heptadecafluorodecyl acrylate/vinylanthracene as dual monomers. The structure, morphology, and magnetic properties of MIA fabricated were investigated by various characterization techniques. Under the optimal conditions the prepared MIA presented satisfactory specific recognition performance. The recognition factor and adsorption capacity towards DFBP were 2.7 and 15.9 mg/g, respectively. The specific recognition behaviors of MIA/MSPE towards DFBP were surveyed by means of adsorption kinetics and adsorption isotherms. Combining MSPE with HPLC coupled to a diode array detector (DAD), a sensitive, reliable and anti-interference method for the monitoring of LCMs residuals in various environmental water and soil samples was established. The achieved enrichment factors were 132–248 and 96–204 in water and soil samples, respectively. The corresponding limits of detection were 0.0017–0.0051 μg/L and 0.087–0.28 μg/kg, respectively. Moreover, confirmatory experiments were adopted to inspect the accuracy of the established MIA/MSPE-HPLC/DAD approach. To the best of our knowledge, this is the first time that an imprinted material has been used for specific isolation and capture of LCMs which have been classified as emerging organic pollutants.
Graphical abstract
{"title":"Facile and rapid preparation of fluorinated imprinted adsorbent for magnetic solid phase extraction of liquid–crystal monomers","authors":"YueYue Zhang, Dingliang Tang, Yuanfei Wu, Xiaojia Huang","doi":"10.1007/s00604-024-06851-x","DOIUrl":"10.1007/s00604-024-06851-x","url":null,"abstract":"<div><p> A new fluorinated imprinted adsorbent (MIA) for magnetic solid phase extraction (MSPE) of liquid crystal monomer (LCM) pollutants was one-pot prepared within 3.5 h using 4-[difluoro(3,4,5-trifluorophenoxy)methyl]-3,5-difluoro-4′-ethyl-biphenyl (DFBP) as template and 1H,1H,2H,2H-heptadecafluorodecyl acrylate/vinylanthracene as dual monomers. The structure, morphology, and magnetic properties of MIA fabricated were investigated by various characterization techniques. Under the optimal conditions the prepared MIA presented satisfactory specific recognition performance. The recognition factor and adsorption capacity towards DFBP were 2.7 and 15.9 mg/g, respectively. The specific recognition behaviors of MIA/MSPE towards DFBP were surveyed by means of adsorption kinetics and adsorption isotherms. Combining MSPE with HPLC coupled to a diode array detector (DAD), a sensitive, reliable and anti-interference method for the monitoring of LCMs residuals in various environmental water and soil samples was established. The achieved enrichment factors were 132–248 and 96–204 in water and soil samples, respectively. The corresponding limits of detection were 0.0017–0.0051 μg/L and 0.087–0.28 μg/kg, respectively. Moreover, confirmatory experiments were adopted to inspect the accuracy of the established MIA/MSPE-HPLC/DAD approach. To the best of our knowledge, this is the first time that an imprinted material has been used for specific isolation and capture of LCMs which have been classified as emerging organic pollutants.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 1","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142789209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-05DOI: 10.1007/s00604-024-06864-6
Kaige Yang, Wenchang Fu, Yinjie Ma, Mingyuan Wu, Xinyan Li, Yan Wang
A straightforward capillary isoelectric focusing (cIEF) method is established with a isoelectric point (pI) range spanning from 3.5 to 7.0, exhibiting excellent linearity and repeatability, with an R2 value of 0.9937 and migration time RSDs for all standard proteins below 0.3%. Subsequently, this method was applied to model exosomes derived from cell lines and healthy human serum, and the peak attributions of exosomes were identified using DiI labeled exosomes and lysed exosomes. The reproducibility of this method in exosome detection was also validated, as the RSDs of all pI values were less than 1%. Then, we observed a significant increase in the pI of exosomes with higher cholesterol content, irrespective of whether they originated from cell culture medium or mouse plasma. Notably, serum exosomes from healthy volunteers exhibited higher pI values compared to those from hepatocellular carcinoma patients, suggesting a potential diagnostic perspective for cancer. These findings underscore the significance of pI measurement in reflecting modifications in exosomal lipid membrane composition and their implications in biological functions mediated by exosomes.
Graphical Abstract
{"title":"A simple capillary isoelectric focusing method as the novel strategy for the isoelectric point measurement of exosomes and its application in disease diagnosis","authors":"Kaige Yang, Wenchang Fu, Yinjie Ma, Mingyuan Wu, Xinyan Li, Yan Wang","doi":"10.1007/s00604-024-06864-6","DOIUrl":"10.1007/s00604-024-06864-6","url":null,"abstract":"<div><p>A straightforward capillary isoelectric focusing (cIEF) method is established with a isoelectric point (pI) range spanning from 3.5 to 7.0, exhibiting excellent linearity and repeatability, with an R<sup>2</sup> value of 0.9937 and migration time RSDs for all standard proteins below 0.3%. Subsequently, this method was applied to model exosomes derived from cell lines and healthy human serum, and the peak attributions of exosomes were identified using DiI labeled exosomes and lysed exosomes. The reproducibility of this method in exosome detection was also validated, as the RSDs of all pI values were less than 1%. Then, we observed a significant increase in the pI of exosomes with higher cholesterol content, irrespective of whether they originated from cell culture medium or mouse plasma. Notably, serum exosomes from healthy volunteers exhibited higher pI values compared to those from hepatocellular carcinoma patients, suggesting a potential diagnostic perspective for cancer. These findings underscore the significance of pI measurement in reflecting modifications in exosomal lipid membrane composition and their implications in biological functions mediated by exosomes.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 1","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142778108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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