Microchimica Acta最新文献_第9页

Design and optimization of a novel fluorescent molecularly imprinted polymer for selective niclosamide sensing in real samples: greenness assessment of MIP synthesis using AGREEMIP

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta

Pub Date : 2025-03-19 DOI: 10.1007/s00604-025-07090-4

Kalyani A. Birari, Pravin O. Patil, Mohamad Taleuzzaman, Md Shamsher Alam, Shadma Wahab, Mohammad Khalid, Zamir G. Khan

In the fields of public health, veterinary medicine, and aquaculture, niclosamide (NIC), a common anthelmintic drug, is essential for deworming and treating a variety of illnesses, such as viral infections, metabolic disorders, and cancer. However, NIC can pose potential risks, such as DNA damage and adverse vasodilation effects in vivo. This study introduces an innovative fluorescent nanosensor for the detection of NIC in food samples. The sensor utilises sulphur and nitrogen co-doped carbon dots (S,N-CDs) derived from eco-friendly materials, Turbo bruneus and L-cysteine, encapsulated within a molecularly imprinted polymer (S,N-CDs@MIP). The sensor operates on a “Turn Off” mechanism, where NIC binding results in decreased fluorescence intensity, effectively detecting NIC in the 1–10 µM concentration range. Excellent sensitivity is indicated by the calibration curve, which displays a strong linear connection between NIC concentration and fluorescence intensity (Y = 0.0612x + 0.0689, R² = 0.9996) and a detection limit of 48.1 nM. The sensor is selective, user-friendly, and cost-effective, with NIC recoveries in food samples ranging from 96.33 to 103.23% and low RSD values (0.81 to 4.89%). Moreover, we utilized the AGREEMIP software to assess the greenness of MIP synthesis processes. This tool evaluates 12 criteria encompassing energy requirements, synthesis specifics, and the environmental impact of reaction components, providing a comprehensive measure of the produced MIP's sustainability. Compared to non-imprinted polymers, the S,N-CDs@MIP sensor offers superior optical stability and specificity, making it a promising tool for practical NIC detection in real-world applications.

Graphical Abstract

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引用次数: 0

Visualization of protrusion-localized STAT3 mRNA using a self-powered lipidic nanoflare for predicting hepatocellular carcinoma metastasis

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta

Pub Date : 2025-03-18 DOI: 10.1007/s00604-025-06988-3

Ya Zhang, Ruichao Zeng, Yuanhang Xia, Wei Han, Yifei Luan, Yuheng Zhang, Shijia Wu, Shouhao Wang, Jinyong Wang, Yongping Chen, Dazhi Chen

Cancer cell metastasis is one of the major causes of patients death with hepatocellular carcinoma (HCC). Previous findings demonstrated that protrusion-accumulated STAT3 mRNA is highly related to HCC cell metastasis, making protrusion-localized STAT3 mRNA an ideal biomarker for evaluating HCC cell initiation and progression. A self-powered lipidic nanoflare (SLNF) has been developed for detecting the expression level of protrusion-accumulated STAT3 mRNA in individual HCC cells, which enables accurate prediction of HCC metastasis. The LNF system is a cholesterol micelle decorated with two kinds of DNA probes, a double-stranded response DNA and a single-stranded fuel probe. The cholesterol micelle can be easily assembled from an amphipathic cholesterol-conjugated DNA via hydrophobicity-mediated aggregation, exhibiting a highly efficient cell internalization. Moreover, the compact and high-density arrangement of DNA probes on the surface of cholesterol micelle enhances their biostability. All the above features make the LNF system an ideal approach for intracellular RNA imaging. The assay commences with the binding of STAT3 mRNA to the response DNA, which peels off the waste DNA and exposes the toehold domain. This domain serves as the proximal holding point for the fuel probe to initiate a strand displacement amplification, which is a crucial step in enabling the detection of targets expressed at trace levels, yielding a limit of detection (LOD) of 100 pM at 37 °C within 1.5 h. The SLNF system is expected to provide useful insight into the development of simple and degradation-resistant DNA probes for visual prediction of HCC metastasis, showing potential applications in tumor diagnosis and treatment.

Graphic abstract

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引用次数: 0

Simple and sensitive SERS platform for Staphylococcus aureus one-pot determination by photoactivated CRISPR/Cas12a cascade system and core–shell DNA tetrahedron@AuNP@Fe3O4 reporter

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta

Pub Date : 2025-03-18 DOI: 10.1007/s00604-025-07098-w

Rui Fan, Shihua Luo, Yangfen He, Yunju Xiao, Yuxin Liang, Lifeng Zhang, Wenbin Li, Ye Zhang, Ling Li

Staphylococcus aureus (S. aureus) is a widely prevalent Gram-positive bacteria that can cause serious infections and diseases in humans and other organisms. Timely detection and treatment in clinical settings is crucial for patient safety and public health. However, current methods for S. aureus detection still face some limitations, such as time-consuming operation, false positives, and labor-intensive available methodology with low sensitivity. Therefore, it is particularly important to develop a rapid, simple, sensitive, and cost-effective method for detecting S. aureus. We developed a SERS platform based on allosteric aptamer-triggered catalytic hairpin assembly (CHA) and photoactivated CRISPR/Cas12a reactions, combined with a multifunctional core–shell structure as the SERS reporter, enabling highly sensitive one-pot determination of S. aureus. Compared with traditional two-step and one-pot analysis methods, this strategy offers superior sensitivity and can successfully identify real samples contaminated with S. aureus. The platform utilizes light-controlled CHA and CRISPR/Cas12a reactions, effectively preventing interference between different reaction systems. Therefore, the photoactivated one-pot CHA/Cas12a strategy provides a simple, rapid, highly sensitive, specific, and cost-effective method for one-pot determination of S. aureus in clinical samples.

Graphical abstract

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引用次数: 0

Immunoelectrochemical assessment of human IgE in non-invasive samples of allergic individuals using PdNCs-labelled antibodies

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta

Pub Date : 2025-03-18 DOI: 10.1007/s00604-025-07083-3

Alejandro Rodríguez-Penedo, Estefanía Costa-Rama, Rosario Pereiro, Beatriz Fernández, M. Teresa Fernández-Abedul

The escalating global prevalence of allergies presents a substantial public health challenge. Immunoglobulin E (IgE) serves as a key biomarker for allergic diseases, often measured in blood serum by ELISA immunoassays. Despite recent interest in minimally invasive sampling of biological fluids, the low sample volumes and IgE concentrations demand highly sensitive methodologies, typically confined to centralized laboratories. In this article, a decentralizable approach based on competitive immunoassays using Pd nanocluster (PdNCs)-labelled antibodies for electrochemical detection is proposed. With this aim, PdNCs were successfully bioconjugated with an anti-hIgE antibody to perform competitive immunoassays. To improve the analytical capabilities of the methodology, disposable screen-printed carbon electrodes with dual working electrodes were used for enhancing precision. Prior electrodeposition of PdNCs at − 0.6 V for 90 s significantly improved sensitivity (7.1 µA g ng⁻¹) and lowered the limit of detection (LoD) to 0.3 ng g⁻¹ for PdNCs determination. The use of PdNCs as labels resulted in an improvement in the LoD for IgE determination. Calibration curves performed using competitive immunoassays for IgE determination, ranging from 10⁻⁵ to 10² ng g⁻¹, demonstrated sensitivity comparable to high-tech methods, with a LoD of 0.008 ng g⁻¹ for electrochemical measurements. Bimodal detection of Pd (linear sweep voltammetry and inductively coupled plasma–mass spectrometry) in various biological fluids (saliva, tears, nasal exudate, capillary blood, and blood serum) revealed significant differences in IgE levels between allergic and non-allergic individuals. Notably, capillary blood correlated strongly with serum blood, and a certain correlation has also been found with nasal exudate. The electrochemical approach, combining sensitivity and precision with non-invasive sampling, offers a simplified alternative for IgE determination in allergic disease.

Graphical Abstract

{"title":"Immunoelectrochemical assessment of human IgE in non-invasive samples of allergic individuals using PdNCs-labelled antibodies","authors":"Alejandro Rodríguez-Penedo, Estefanía Costa-Rama, Rosario Pereiro, Beatriz Fernández, M. Teresa Fernández-Abedul","doi":"10.1007/s00604-025-07083-3","DOIUrl":"10.1007/s00604-025-07083-3","url":null,"abstract":"<div><p>The escalating global prevalence of allergies presents a substantial public health challenge. Immunoglobulin E (IgE) serves as a key biomarker for allergic diseases, often measured in blood serum by ELISA immunoassays. Despite recent interest in minimally invasive sampling of biological fluids, the low sample volumes and IgE concentrations demand highly sensitive methodologies, typically confined to centralized laboratories. In this article, a decentralizable approach based on competitive immunoassays using Pd nanocluster (PdNCs)-labelled antibodies for electrochemical detection is proposed. With this aim, PdNCs were successfully bioconjugated with an anti-hIgE antibody to perform competitive immunoassays. To improve the analytical capabilities of the methodology, disposable screen-printed carbon electrodes with dual working electrodes were used for enhancing precision. Prior electrodeposition of PdNCs at − 0.6 V for 90 s significantly improved sensitivity (7.1 µA g ng⁻<sup>1</sup>) and lowered the limit of detection (LoD) to 0.3 ng g⁻<sup>1</sup> for PdNCs determination. The use of PdNCs as labels resulted in an improvement in the LoD for IgE determination. Calibration curves performed using competitive immunoassays for IgE determination, ranging from 10<sup>−5</sup> to 10<sup>2</sup> ng g<sup>−1</sup>, demonstrated sensitivity comparable to high-tech methods, with a LoD of 0.008 ng g<sup>−1</sup> for electrochemical measurements. Bimodal detection of Pd (linear sweep voltammetry and inductively coupled plasma–mass spectrometry) in various biological fluids (saliva, tears, nasal exudate, capillary blood, and blood serum) revealed significant differences in IgE levels between allergic and non-allergic individuals. Notably, capillary blood correlated strongly with serum blood, and a certain correlation has also been found with nasal exudate. The electrochemical approach, combining sensitivity and precision with non-invasive sampling, offers a simplified alternative for IgE determination in allergic disease.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 4","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00604-025-07083-3.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143638632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cadmium telluride quantum dot-MXene composite–based electrochemical sensing platform for simultaneous determination of rutin and quercetin in foods

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta

Pub Date : 2025-03-18 DOI: 10.1007/s00604-025-07092-2

Lin Li, Lanlan Liu, Jianmei Zhou, Chen Gu, Xiongzhi Wu, Chenghong Lei, Liqiang Yan

A simple and rapid electrochemical method based on a composite of cadmium telluride quantum dots (CdTe QDs) and MXene is developed for the simultaneous determination of rutin and quercetin in food samples. The CdTe QD-MXene composite is synthesized via the in situ growth of CdTe QDs on MXene, which serves as a carrier and enhances electrical conductivity. Incorporating CdTe QDs into MXene interlayers effectively prevents agglomeration in MXene and provides more active sites for electrochemical determination. The developed electrochemical method can successfully determine rutin and quercetin, both individually and simultaneously, in aqueous solutions while achieving high stability and selectivity. Notably, the prepared sensor exhibits limits of detection of 3.300 × 10⁻⁸ and 3.268 × 10⁻⁷ M for the simultaneous determination of rutin and quercetin, respectively. Moreover, the sensing platform is used for the determination of rutin in buckwheat, locust rice, and apples, with results well comparable to those obtained using ultraviolet spectroscopy. Finally, the proposed sensor is employed to monitor the hydrolysis of rutin into quercetin in buckwheat using an electrochemical method for the first time. This study provides new ideas for the application of electrochemical sensors in food and drug science.

Graphical Abstract

{"title":"Cadmium telluride quantum dot-MXene composite–based electrochemical sensing platform for simultaneous determination of rutin and quercetin in foods","authors":"Lin Li, Lanlan Liu, Jianmei Zhou, Chen Gu, Xiongzhi Wu, Chenghong Lei, Liqiang Yan","doi":"10.1007/s00604-025-07092-2","DOIUrl":"10.1007/s00604-025-07092-2","url":null,"abstract":"<div><p> A simple and rapid electrochemical method based on a composite of cadmium telluride quantum dots (CdTe QDs) and MXene is developed for the simultaneous determination of rutin and quercetin in food samples. The CdTe QD-MXene composite is synthesized via the in situ growth of CdTe QDs on MXene, which serves as a carrier and enhances electrical conductivity. Incorporating CdTe QDs into MXene interlayers effectively prevents agglomeration in MXene and provides more active sites for electrochemical determination. The developed electrochemical method can successfully determine rutin and quercetin, both individually and simultaneously, in aqueous solutions while achieving high stability and selectivity. Notably, the prepared sensor exhibits limits of detection of 3.300 × 10<sup>−8</sup> and 3.268 × 10<sup>−7</sup> M for the simultaneous determination of rutin and quercetin, respectively. Moreover, the sensing platform is used for the determination of rutin in buckwheat, locust rice, and apples, with results well comparable to those obtained using ultraviolet spectroscopy. Finally, the proposed sensor is employed to monitor the hydrolysis of rutin into quercetin in buckwheat using an electrochemical method for the first time. This study provides new ideas for the application of electrochemical sensors in food and drug science.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 4","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143645539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrochemical sensing of caffeic acid on natural biomass-pyrrole-functionalized magnetic biochar (PFMB) as promising SPE material

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta

Pub Date : 2025-03-18 DOI: 10.1007/s00604-025-07087-z

Imen Abidli, Mohamed Bououdina, Latifa Latrous, Adel Megriche

A peanut shell-modified screen-printed carbon electrode (SPE) was developed for the sensing of caffeic acid (CA) in saliva samples using cheap miniaturized analyzer composed of a laptop and an electrochemical workstation. Peanut shells, sourced from abundant biomass residues, were used to fabricate magnetic biochar (MB) and pyrrole-functionalized magnetic biochar (PFMB) with varying pyrrole/Fe ratios through a hydrothermal process. The surface morphology and electrochemical properties of the synthesized PFMB material were analyzed using XRD, FTIR, Raman, SEM, VSM, cyclic voltammetry, and differential pulse voltammetry techniques. The PFMB-modified SPE displayed excellent electrocatalytic response towards CA in a wide linear range from 10 to 600 μM with a low limit of detection of 0.08 μM. The enhanced electrocatalytic response could be ascribed to the synergistic effect of pyrrole-functionalized biochar and Fe₃O₄ on the newly designed probe. Moreover, the fabricated sensor was successfully utilized for real-time detection of CA in various samples. Quantum chemical modeling was performed to confirm the relevant findings to clarify the structure–activity relationship of CA adsorption on biochar.

Graphical Abstract

{"title":"Electrochemical sensing of caffeic acid on natural biomass-pyrrole-functionalized magnetic biochar (PFMB) as promising SPE material","authors":"Imen Abidli, Mohamed Bououdina, Latifa Latrous, Adel Megriche","doi":"10.1007/s00604-025-07087-z","DOIUrl":"10.1007/s00604-025-07087-z","url":null,"abstract":"<div><p>A peanut shell-modified screen-printed carbon electrode (SPE) was developed for the sensing of caffeic acid (CA) in saliva samples using cheap miniaturized analyzer composed of a laptop and an electrochemical workstation. Peanut shells, sourced from abundant biomass residues, were used to fabricate magnetic biochar (MB) and pyrrole-functionalized magnetic biochar (PFMB) with varying pyrrole/Fe ratios through a hydrothermal process. The surface morphology and electrochemical properties of the synthesized PFMB material were analyzed using XRD, FTIR, Raman, SEM, VSM, cyclic voltammetry, and differential pulse voltammetry techniques. The PFMB-modified SPE displayed excellent electrocatalytic response towards CA in a wide linear range from 10 to 600 μM with a low limit of detection of 0.08 μM. The enhanced electrocatalytic response could be ascribed to the synergistic effect of pyrrole-functionalized biochar and Fe<sub>3</sub>O<sub>4</sub> on the newly designed probe. Moreover, the fabricated sensor was successfully utilized for real-time detection of CA in various samples. Quantum chemical modeling was performed to confirm the relevant findings to clarify the structure–activity relationship of CA adsorption on biochar.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 4","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143645537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Facile preparation of polymer dots for tetracycline and Al3+ detection and exploration of anti-counterfeiting applications via the fluorescence “ON–OFF-ON” strategy

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta

Pub Date : 2025-03-17 DOI: 10.1007/s00604-025-07093-1

Zhonghao Li, Zherui Wan, Xiaoxu Cui, Qi Chen, Yue Hua, Guang Yang

Polymer dots (PT-PDs) were obtained through a “synthesis-modification integration” method by using polyethyleneimine (PEI) and tartaric acid (TA) as raw materials. The designed PT-PDs displayed a nanoscale structure with an average size of 1.6 nm, and bright blue fluorescence (FL) emission with a fluorescence quantum yield (QY) of 14.3%. Moreover, the PT-PDs were used as a sensing platform for the sensitive and quantitative detection of tetracyclines (TCs) and Al³⁺ via fluorescence quenching and recovery. The LODs for tetracycline hydrochloride (TCH), doxycycline (DOX), and Al³⁺ were 94.8 nM, 76.7 nM, and 177.8 nM, respectively. In addition, PT-PDs incorporated with polyacrylamide were used for the recognition of TCs and Al³⁺ in a portable manner on the basis of the fluorescence “ON–OFF-ON” strategy, which revealed great application in the field of anti-counterfeiting and encryption.

Graphical Abstract

{"title":"Facile preparation of polymer dots for tetracycline and Al3+ detection and exploration of anti-counterfeiting applications via the fluorescence “ON–OFF-ON” strategy","authors":"Zhonghao Li, Zherui Wan, Xiaoxu Cui, Qi Chen, Yue Hua, Guang Yang","doi":"10.1007/s00604-025-07093-1","DOIUrl":"10.1007/s00604-025-07093-1","url":null,"abstract":"<div><p>Polymer dots (PT-PDs) were obtained through a “synthesis-modification integration” method by using polyethyleneimine (PEI) and tartaric acid (TA) as raw materials. The designed PT-PDs displayed a nanoscale structure with an average size of 1.6 nm, and bright blue fluorescence (FL) emission with a fluorescence quantum yield (QY) of 14.3%. Moreover, the PT-PDs were used as a sensing platform for the sensitive and quantitative detection of tetracyclines (TCs) and Al<sup>3+</sup> via fluorescence quenching and recovery. The LODs for tetracycline hydrochloride (TCH), doxycycline (DOX), and Al<sup>3+</sup> were 94.8 nM, 76.7 nM, and 177.8 nM, respectively. In addition, PT-PDs incorporated with polyacrylamide were used for the recognition of TCs and Al<sup>3+</sup> in a portable manner on the basis of the fluorescence “ON–OFF-ON” strategy, which revealed great application in the field of anti-counterfeiting and encryption.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 4","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143632386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Casein-Tb-Ti3C2 quantum dots ratiometric fluorescence probe for the detection of ciprofloxacin

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta

Pub Date : 2025-03-17 DOI: 10.1007/s00604-025-07107-y

Chong Wang, Huixin Wang, Qingting Ni, Wenjuan Zhou, Dan Liu

A water-stable ratiometric fluorescence probe based on casein-Tb-Ti₃C₂ quantum dots was developed for the highly sensitive and selective detection of ciprofloxacin. With the increase in ciprofoxacin concentration, the fluorescence intensity at 440 nm remained relatively stable, while the fluorescence emission at 543 nm exhibited progressive enhancement. This may be attributed to ciprofloxacin being able to effectively coordinate with Tb³⁺ ions while displacing surrounding water molecules, leading to enhanced luminescence. Thus, the I₅₄₃/I₄₄₀ ratio was utilized as an indicator for monitoring changes in ciprofloxacin concentration. The ratiometric fluorescence probe demonstrated excellent linearity across a concentration range 0.1–800 µM and exhibited high sensitivity, achieving a detection limit of 0.018 μM for ciprofloxacin. Furthermore, the ratiometric fluorescence probe was successfully employed for the detection of ciprofloxacin in pork, beef, fish, honey, eggs, and milk samples, achieving recoveries that ranged from 76.5 to 123%. Owing to its advantages of high sensitivity and excellent selectivity, the ratiometric fluorescence probe demonstrates significant potential for practical applications in ciprofloxacin detection.

Graphical abstract

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引用次数: 0

Intramolecular enhanced entropy-driven DNA-Au nanodevice for mRNA imaging in living cells

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta

Pub Date : 2025-03-17 DOI: 10.1007/s00604-025-06997-2

Chao Guo, Tongnian Gu, Shao-Hua Wen, Yuan Dang, Yuanzhen Zhou, Junping Ma, Sha Yu

An intramolecular enhanced entropy-driven DNA amplifier-tethered gold nanoparticle (DNA-Au) nanodevice has been designed for highly sensitive in situ imaging of messenger ribonucleic acid (mRNA) in living cells. The DNA amplifier is immobilized on a same AuNP and the initial fluorescence of DNA-Au nanodevice is quenched. Upon internalized into the target cancer cells, the nanodevice can be activated by endogenous TK1 mRNA, and promptly release the fluorophore via the intramolecular enhanced DNA strand displacement reaction. The decreasing distance and increasing local concentration of the probes via intramolecular reaction can significantly improve the reaction kinetics of DNA-Au nanodevice, thus achieving the highly sensitive imaging of TK1 mRNA. The excellent sensitivity and selectivity allow the DNA-Au nanodevice to accurately discriminate different cell lines and monitor the variations in intracellular TK1 mRNA expression levels via fluorescence imaging. Therefore, the proposed intramolecular enhanced entropy-driven DNA-Au nanodevice will afford a reliable approach for accurate determination of mRNA in molecular diagnostic systems.

Graphical Abstract

{"title":"Intramolecular enhanced entropy-driven DNA-Au nanodevice for mRNA imaging in living cells","authors":"Chao Guo, Tongnian Gu, Shao-Hua Wen, Yuan Dang, Yuanzhen Zhou, Junping Ma, Sha Yu","doi":"10.1007/s00604-025-06997-2","DOIUrl":"10.1007/s00604-025-06997-2","url":null,"abstract":"<div><p>An intramolecular enhanced entropy-driven DNA amplifier-tethered gold nanoparticle (DNA-Au) nanodevice has been designed for highly sensitive in situ imaging of messenger ribonucleic acid (mRNA) in living cells. The DNA amplifier is immobilized on a same AuNP and the initial fluorescence of DNA-Au nanodevice is quenched. Upon internalized into the target cancer cells, the nanodevice can be activated by endogenous TK1 mRNA, and promptly release the fluorophore via the intramolecular enhanced DNA strand displacement reaction. The decreasing distance and increasing local concentration of the probes via intramolecular reaction can significantly improve the reaction kinetics of DNA-Au nanodevice, thus achieving the highly sensitive imaging of TK1 mRNA. The excellent sensitivity and selectivity allow the DNA-Au nanodevice to accurately discriminate different cell lines and monitor the variations in intracellular TK1 mRNA expression levels via fluorescence imaging. Therefore, the proposed intramolecular enhanced entropy-driven DNA-Au nanodevice will afford a reliable approach for accurate determination of mRNA in molecular diagnostic systems.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 4","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143638631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Capillary-driven distance-based paper analytical devices for albumin protein and glucose quantification in human whole blood

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta

Pub Date : 2025-03-15 DOI: 10.1007/s00604-025-07079-z

Kawin Khachornsakkul, Thithawat Trakoolwilaiwan, Ruben Del-Rio-Ruiz, Sameer Sonkusale, Tapparath Leelasattarathkul

This study presents a simple and inexpensive distance-based paper analytical device (dPAD) for plasma separation from whole blood samples and its application in monitoring albumin protein and glucose levels using colorimetric and fluorescent distance methods. The developed dPAD consists of a sample zone, a separation zone with hydrophobic wax-patterned lines, a pretreatment zone, and a straight zone channel pre-deposited with chemical reagents for both albumin protein and glucose quantification. Plasma separation relies on the capillarity-driven different flow velocities of blood cells and plasma with varying hydrophilicity in the paper channel. Remarkably, the blood cells are trapped in the separation channel of the device, while plasma can be separated and subsequently flow with a buffer solution to the detection zone by capillary force. Target analyte in plasma content then reacts with its specific reagents, resulting in the change in the color or fluorescent distance signal. Our sensor exhibited remarkable accuracy and precision for the detection of albumin protein and glucose in whole blood samples with an acceptable recovery range between 99.94 and 101.65% and the highest relative standard deviation (RSD) of 4.49%. Furthermore, the results indicated no significant differences between our method and conventional methods for albumin protein and glucose determination in whole blood samples. Additionally, to the best of our knowledge, this method is the first time for the development of the fluorescent dPAD sensor for glucose monitoring. It is also the first demonstration to use a dPAD sensor for the direct detection of both albumin protein and glucose levels in whole blood. Hence, despite its simplicity, the concept offers a more cost-effective and accessible method for plasma separation from whole blood and subsequent albumin protein or and glucose detection. Moreover, it can be extended for further advancements in POC analytical sensing.

Graphical Abstract

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引用次数: 0