Microchimica Acta最新文献

英文中文

Modified Carba PBP test for rapid detection and differentiation between different classes of carbapenemases in Enterobacterales

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta

Pub Date : 2024-12-05 DOI: 10.1007/s00604-024-06859-3

Xiaonan Wang, Zhimin Lu, Leina Dou, Licai Ma, Tong He, Chenxi Gao, Xiangjun Zhao, Jin Tao, Liang Luo, Qing Li, Yang Wang, Yingbo Shen, Jianzhong Shen, Zhanhui Wang, Kai Wen

An advanced biochemical assay named modified Carba PBP test was innovated to identify and differentiate distinct categories of clinically significant carbapenemases (Ambler classes A, B, and D) within the Enterobacterales. The mechanism of mCarba PBP hinges on two core attributes: (i) the hydrolysis of the meropenem substrate by various carbapenemases, (ii) the immobilized penicillin and free meropenem in their affinity to interact with a limited quantity of penicillin-binding protein (PBP). Specific inhibitors for class A (phenylboronic acid, PBA) and class B (ethylenediaminetetraacetic acid, EDTA) were employed to inhibit the hydrolysis activity of carbapenemase and facilitate the classification of carbapenemase classes within 25 min. A comprehensive evaluation was undertaken using 94 clinical Enterobacterales isolates, comprising 75 carbapenemase-producing strains and 19 non-carbapenemase-producing strains. Its overall specificity and sensitivity were 100% and 97.3%, respectively, including detection of all types of OXA-48-like carbapenemases. For precise carbapenemase type identification, the assay exhibited remarkable sensitivities for class A, class B, and class D detection at 94.7%, 100%, and 100%, respectively. This user-friendly test presents a promising tool for carbapenemase identification, refining the selection of β-lactam/β-endoenzyme inhibitor combinations for effectively treating infections due to carbapenemase-producing organisms.

Graphical Abstract

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引用次数: 0

Detection of Aβ40 in cerebrospinal fluid and plasma of Alzheimer’s disease patients using photoelectrochemical biosensors

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta

Pub Date : 2024-12-04 DOI: 10.1007/s00604-024-06816-0

Liting Li, Na Wei, Yiwei Guo, Xingyu Zhu, Lin Wang, Yanzhao Zhu, Ke Fang, Shenhui Ma, Yingying Zhang, Yan Zhang, Xiaofei Zhou, Gang Zhao, Yuyu Bu, Linfu Zhou

An ultra-sensitive photoelectrochemical (PEC) biosensor for amyloid-beta 40 (Aβ40), a biomarker for Alzheimer’s disease (AD), was developed using g-C₃N₄ modified with gold nanoparticles (Au NPs) to form Au-C₃N₄. This was further combined with TiO₂ to create a tightly bonded TiO₂/Au-C₃N₄ heterojunction, leading to a highly responsive photocatalytic process. Furthermore, the incorporation of noble metal Au NPs not only enhances photocurrent generation but also securely immobilizes the aptamer through Au–S bonds, providing additional surface binding sites. This significantly increases the sensor's capture efficiency. The sensor exhibited excellent performance, featuring a linear detection range from 10⁻¹⁵ to 10⁻¹¹ g/mL and a remarkably low detection limit (LOD) of 0.33 fg/mL. Moreover, the validation in clinical settings demonstrated the successful detection in real cerebrospinal fluid (CSF) and plasma, from AD patients and non-AD controls. These results strongly suggest that PEC biosensors possess significant potential as cost-effective and highly sensitive tools for detecting ultra-trace substances in human body fluids, which offers promising opportunities for the early screening of high-risk populations for AD.

Graphical Abstract

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引用次数: 0

Correction: Molecularly imprinted electrochemical sensor based on APTES-functionalized indium tin oxide electrode for the determination of sulfadiazine

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta

Pub Date : 2024-12-04 DOI: 10.1007/s00604-024-06866-4

Samridhi Chopra, Manisha Balkhandia, Manisha Khatak, Navya Sagar, Ved Varun Agrawal

引用次数: 0

Electrochemical immunosensing of Crohn’s disease biomarkers using diazonium salt-grafted crystalline nanocellulose/carbon nanotube-modified electrodes

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta

Pub Date : 2024-12-04 DOI: 10.1007/s00604-024-06868-2

Lorena García-Rodrigo, Claudia Ramos-López, Esther Sánchez-Tirado, Lourdes Agüí, Araceli González-Cortés, Paloma Yáñez-Sedeño, José M. Pingarrón

The first dual immunosensor is reported for the determination of IL-12 and IL-23, two relevant biomarkers of Crohn’s disease (CD). The strategy relies on the selective capture of the targets by the respective antibodies which were covalently immobilized onto SPCEs modified with crystalline nanocellulose (CNC) and multi-walled carbon nanotubes (MWCNTs) followed by conjugation with a detector antibody labelled with poly-HRP-Strept and amperometric transduction using the H₂O₂/HQ system. The combination of CNC, a nanomaterial scarcely exploited in immunosensing, with MWCNTs enables the preparation of a novel dual immunosensor for the determination of CD biomarkers in clinical samples, including faeces. The developed bioplatform exhibits a high reproducibility and selectivity allowing the simultaneous determination of IL-12 and IL-23 using a smaller sample volume and in a much shorter assay time than those reported for each target with commercial ELISA kits. The dual immunosensor was successfully applied to the analysis of raw serum and faeces spiked with IL-12 and IL-23 at the expected levels in patients with severe CD.

Graphical Abstract

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引用次数: 0

Electrochemical sensor based on molecularly imprinted polypyrrole-MWCNTs-OH/covalent organic framework for the detection of ofloxacin in water

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta

Pub Date : 2024-12-03 DOI: 10.1007/s00604-024-06860-w

Lijuan Huang, Yuan Luo, Xulin Li, Juan Wu, Qian Long, Li Zheng, Wenlong Liao, Huiming Li, Lingpu Jia, Kunping Liu

A platform was developed to accurately detect the content of ofloxacin (OFX) based on molecularly imprinted polypyrrole-MWCNTs-OH/1,3,5-Tris(4-aminophenyl) benzene (TAPB)-2,5-dimethoxybenzene-1,4-dicarboxaldehyde (DMTP)-covalent organic framework (MIP-MWCNTs-OH/COF)–modified glassy carbon electrode (GCE) sensor (MIP-MWCNTs-OH/COF/GCE). The complex of MWCNTs-OH and COF synergistically enhanced the active area and electrochemical signal, based on which a molecularly imprinted membrane was polymerized on its surface to further improve the selectivity. Under optimized conditions, the prepared MIP-MWCNTs-OH/COF/GCE sensor exhibited strong detection performance to OFX in a linear range 1.969 × 10⁻¹¹–9.619 × 10⁻⁹ M with the limit of detection (LOD, 3S/N) of 4.989 × 10⁻¹² M, excellent selectivity, stability, and reproducibility. Furthermore, the MIP-MWCNTs-OH/COF/GCE sensor can be successfully applied to the detection of OFX in lake water and eye drops with a relative standard deviation (RSD) of less than 4.95%, indicating its high potential in practical applications.

Graphical Abstract

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引用次数: 0

Colorimetric sensor array for sensitive detection and identification of bacteria based on the etching of triangular silver nanoparticles regulated by Cl− at various concentrations

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta

Pub Date : 2024-12-02 DOI: 10.1007/s00604-024-06855-7

Shuang Liu, Peng Yan, Shahzad Naveed, Yuheng Zhu, Tao Fu, Ruijing Wu, Yayan Wu

A colorimetric sensor array is proposed for ultrasensitive detection and identification of bacteria by using Cl⁻ at various concentrations as sensing elements and triangular silver nanoparticles (T-AgNPs) as a single sensing nanoprobe. T-AgNPs are easily etched by Cl⁻. However, in the presence of bacteria, the etching process will be hindered. Different bacteria have differential protective effects on T-AgNPs due to their interactions, resulting in different etching degrees of T-AgNPs by Cl⁻, and visual color changes. By adjusting the antagonistic action between bacteria protection on T-AgNPs and the etching by using Cl⁻ at various concentrations, different bacteria had their own color response patterns. Combined with linear discriminant analysis (LDA) and orthogonal partial least squares-discriminant analysis (OPLS-DA), the bacteria could be identified. The method was also used for bacteria mixtures identification and showed high sensitivity (OD₆₀₀ = 1.0 × 10⁻⁶) for V. parahaemolyticus detection. Finally, the sensor array was successfully utilized in the identification of bacteria in pure and mineral bottled water. The method is low-cost, simple, sensitive, visual, and has potential application in point-of-care testing of bacteria.

Graphical Abstract

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引用次数: 0

Antenna effect on Zn(II) porphyrin–based molecular ensembles for the detection of 2,4-dinitrophenol through energy and electron transfer process

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta

Pub Date : 2024-12-02 DOI: 10.1007/s00604-024-06795-2

Prasanth Palanisamy, Mageshwari Anandan, Gurusamy Raman, Venkatramaiah Nutalapati

Two modular systems were synthesized composed of triphenylamine (ZnTPAP) and pyrene (ZnPyP) covalently linked at meso position of the Zn(II) porphyrins. Both compounds behaved as energy transfer antenna and orthogonal units to enhance the electron donating ability of Zn(II) porphyrins. Detailed photophysical and aggregation studies reveal that an appreciable electronic interaction exists between peripheral units to the porphyrin π-system so that they behave like strong donor materials. The electrochemical and computational studies demonstrate delocalization of the frontier highest occupied molecular orbital (−5.08 eV) over the triphenylamine entities (ZnTPAP) in addition to the porphyrin macrocycle. Fluorescence experiments with ZnTPAP and ZnPyP in the presence of different nitro analytes at various concentrations show turn-off fluorescence behaviour and exhibit superior selectivity towards 2,4-dinitrophenol (DNP) with limit of detection (LOD) of ~ 2.3 and 9.2 ppm for ZnTPAP and ZnPyP. Photoinduced electron transfer process is involved in the static and dynamic fluorescence quenching process. A Stern–Volmer quenching association constant (K_sv) determination revealed that ZnTPAP is more sensitive than the ZnPyP. This is attributed to the strong donating behaviour of TPA units caused by intermolecular interaction through metal center and strong π–π interactions with nitro analytes. The present study provides new insights into the ability to tune the affinity and selectivity of porphyrin-based sensors utilising electronic factors associated with the central Zn(II) ion. Furthermore, a smartphone-interfaced portable fluorimetric method by recognising colour variations in RGB and the luminance (L) values facilitate sensitive and real-time sensing at low concentration levels will have a significant impact on development of a new class of chemosensors.

Graphical Abstract

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引用次数: 0

A facile synthesis of polyethyleneimine based fluorescent polymeric nanorods using vitamin B6 cofactor as cross-linker for ratiometric detection of doxorubicin

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta

Pub Date : 2024-11-29 DOI: 10.1007/s00604-024-06856-6

Anuj K Saini, Ritambhara Jangir, Bigyan Ranjan Jali, Suban K Sahoo

Polyethyleneimine (PEI) based fluorescent polymeric nanorods with high quantum yield (87.21%) were synthesized by crosslinking and self-assembly of PEI by vitamin B₆ cofactor pyridoxal 5’-phosphate (PLP). The fluorescent PEIPLP polymeric nanorods were employed for the ratiometric detection of doxorubicin (DOX). With the addition of DOX to the PEIPLP nanorods, the fluorescent intensity was decreased at 510 nm and concomitantly enhanced at 590 nm due to the fluorescence resonance energy transfer (FRET) process. The selective and specific ratiometric fluorescence response from the PEIPLP nanorods can be employed to detect DOX down to 10 nM. The practical utility of the PEIPLP nanorods was examined by detecting DOX in human serum.

Graphical Abstract

引用次数: 0

Recent progress of black phosphorene from preparation to diversified bio-/chemo-nanosensors and their challenges and opportunities for comprehensive health 黑色磷烯从制备到多样化生物/化学纳米传感器的最新进展及其对全面健康的挑战和机遇

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta

Pub Date : 2024-11-29 DOI: 10.1007/s00604-024-06828-w

Ting Xue, Xinyu Lu, Yangping Wen, Hassan Karimi Maleh, Xuemin Duan, Jingkun Xu

The introduction of comprehensive health, related to human living environment and mental state, helps people to improve human health literacy and accept scientific health guidance. The unique structure and properties of black phosphorene (BP) provide potential opportunities for rapid development and versatile applications of high-performance sensors serving comprehensive health. The review begins with the preparation from bulk black phosphorous crystals via transforming requirements of phosphorous allotropes and BP nanosheets via preparative strategies using both “top-down” and “bottom-up” methods. Then the diversified modification of BP and versatile fabrication of diversified bio-/chemo-nanosensors for sensitive detection of analytes are discussed. Besides, the challenges including the preparation of BP, diversified modification, devices for improving performance defects and chemo-/bio-nanosensors for enhancing performance are outlined together with potential opportunities for the BP preparation and applications in comprehensive health from agricultural environments, food safety, personal life, physical and mental life, and finally to medical care.

Graphical Abstract

全面健康的提出，关系到人类的生存环境和精神状态，有助于人们提高人类健康素养，接受科学的健康指导。黑磷（BP）的独特结构和性能为服务于全面健康的高性能传感器的快速发展和广泛应用提供了潜在的机遇。本综述首先介绍了通过 "自上而下 "和 "自下而上 "的制备策略，从块状黑磷晶体出发，通过对磷的同素异形体和 BP 纳米片的转化需求制备 BP 纳米片。然后讨论了 BP 的多样化改性和用于灵敏检测分析物的多样化生物/化学纳米传感器的多功能制造。此外，还概述了 BP 的制备、多样化改性、改善性能缺陷的装置和提高性能的化学/生物纳米传感器等方面的挑战，以及 BP 制备和应用于从农业环境、食品安全、个人生活、身心健康到医疗保健等全面健康领域的潜在机遇。

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引用次数: 0

Establishment of a visual assessment platform for total antioxidant capacity of Ganoderma sichuanense based on Arg-CeO2 hydrogel nanozyme utilizing a self-programmed smartphone app and a self-designed 3D-printed device 基于 Arg-CeO2 水凝胶纳米酶的灵芝总抗氧化能力可视化评估平台的建立--利用自编程智能手机应用程序和自行设计的 3D 打印设备

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta

Pub Date : 2024-11-29 DOI: 10.1007/s00604-024-06840-0

Suhui Tan, Mengyuan Tan, Tao Zhang, Lu Gao, Yi Li, Xuechao Xu, Zhen-quan Yang

A multienzyme-like L-argininemodified CeO₂ (Arg-CeO₂) nanozyme was prepared for accurate total antioxidant capacity (TAC) determination of Ganoderma sichuanense (G. sichuanense). It exhibits oxidase-like and peroxidase-like activities catalyzing the generation of superoxide anion free radicals and hydroxyl radicals, accelerating 3, 3′, 5, 5′-tetramethylbenzidine (TMB) chromogenic reaction. Antioxidants can effectively eliminate the generated free radicals, inhibiting the TMB chromogenic reaction. Hence, TAC can be assessed by Arg-CeO₂ catalyzed TMB chromogenic reaction using ascorbic acid (AA) as a reference substance. TAC of G. sichuanense extracted using different solvents was determined, indicating the highest TAC observed in the G. sichuanense extracted by the mixture solvent (deionized water/ethanol/ethyl acetate = 1 : 1 : 1). A visual assessment platform based on Arg-CeO₂ hydrogel nanozyme utilizing a self-programmed smartphone app and a self-designed 3D printed device is established. TAC using AA as a reference substance (3.33 ~ 83.33 µM) can be detected with a detection limit as low as 0.58 µM. The platform can realize one-step quantitative determination of TAC in G. sichuanense within 40 min. The proposed visual assessment platform presented exhibits promising application prospects in the field of TAC assessment in food.

Graphical Abstract

制备了一种多酶样 L-精氨酸修饰 CeO2（Arg-CeO2）纳米酶，用于准确测定四川灵芝的总抗氧化能力（TAC）。Arg-CeO2 纳米酶具有类似氧化酶和过氧化物酶的活性，能催化超氧阴离子自由基和羟自由基的生成，加速 3，3′，5，5′-四甲基联苯胺（TMB）显色反应。抗氧化剂能有效消除产生的自由基，抑制 TMB 致色反应。因此，可以用抗坏血酸（AA）作为参照物，通过 Arg-CeO2 催化的 TMB 致色反应来评估 TAC。测定了不同溶剂萃取的川贝母的 TAC，结果表明混合溶剂（去离子水/乙醇/乙酸乙酯 = 1 : 1 : 1）萃取的川贝母的 TAC 最高。基于 Arg-CeO2 水凝胶纳米酶，利用自编程智能手机应用程序和自行设计的 3D 打印设备，建立了一个可视化评估平台。以 AA 为参考物质（3.33 ~ 83.33 µM）检测 TAC，检测限低至 0.58 µM。该平台可在 40 分钟内实现川贝母中 TAC 的一步定量检测。所提出的可视化评估平台在食品中TAC评估领域具有广阔的应用前景。图文摘要

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