Pub Date : 2022-10-28DOI: 10.4025/actascibiolsci.v44i1.62512
A. Andrade, P. E. C. E. Silva, Rebeca Gonçalves de Melo, Millena Patrício do Nascimento Ferreira, A. Porto, R. Bezerra
Microalgae are known for producing various biotechnological products. Moreover, they absorb nutrients from dairy wastewater, grow well, and accumulate valuable compounds faster. In this study, photoautotrophic and mixotrophic cultivation with different initial lactose concentrations present in cheese whey (CW) were established to investigate their effect on cell concentration (Xm, mg L-1), cell productivity (Px, mg L-1day-1), and specific cell growth (µmax, day-1) of Chlorella vulgaris, Dunaliella tertiolecta, and Tetradesmus obliquus. The biomass production of C. vulgaris (Xm= 1,520 ± 30.3 mg L-1, Px = 147 ± 3.00 mg L-1, and µmax = 0.150 ± 0.00 mg L-1) in mixotrophic culture with 10.0 g L-1 of lactose, the main constituent of CW, was notably enhanced by 55% in comparison with their photoautotrophic cultures, whereas a lower effect of these lactose concentrations on cell growth was observed in T. obliquus and D. tertiolecta. Thus, mixotrophic cultivation of C. vulgaris using CW as a carbon and energy source could be considered a feasible alternative to obtain high value-added biomass.
{"title":"Microalgal production under mixotrophic conditions using cheese whey as substrate","authors":"A. Andrade, P. E. C. E. Silva, Rebeca Gonçalves de Melo, Millena Patrício do Nascimento Ferreira, A. Porto, R. Bezerra","doi":"10.4025/actascibiolsci.v44i1.62512","DOIUrl":"https://doi.org/10.4025/actascibiolsci.v44i1.62512","url":null,"abstract":"Microalgae are known for producing various biotechnological products. Moreover, they absorb nutrients from dairy wastewater, grow well, and accumulate valuable compounds faster. In this study, photoautotrophic and mixotrophic cultivation with different initial lactose concentrations present in cheese whey (CW) were established to investigate their effect on cell concentration (Xm, mg L-1), cell productivity (Px, mg L-1day-1), and specific cell growth (µmax, day-1) of Chlorella vulgaris, Dunaliella tertiolecta, and Tetradesmus obliquus. The biomass production of C. vulgaris (Xm= 1,520 ± 30.3 mg L-1, Px = 147 ± 3.00 mg L-1, and µmax = 0.150 ± 0.00 mg L-1) in mixotrophic culture with 10.0 g L-1 of lactose, the main constituent of CW, was notably enhanced by 55% in comparison with their photoautotrophic cultures, whereas a lower effect of these lactose concentrations on cell growth was observed in T. obliquus and D. tertiolecta. Thus, mixotrophic cultivation of C. vulgaris using CW as a carbon and energy source could be considered a feasible alternative to obtain high value-added biomass.","PeriodicalId":7166,"journal":{"name":"Acta Scientiarum. Biological Sciences","volume":"89 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74016537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-28DOI: 10.4025/actascibiolsci.v44i1.61495
Vinícius da Silva Rasvailer, Camila Gentilin-Avanci, M. Scoarize, G. D. Pinha, E. Benedito
Small rivers, henceforth streams, depend on organic matter (nutrients and energy) from riparian vegetation. The quality of such allochthonous debris is determinant for the transformation of organic matter compounds, where the bacterial community has a crucial role in the final decomposition of the substrate. During bacterial colonization, debris with higher concentration of nutrients (more palatable) is prioritized, which accelerates the process. This study investigated the effects of leaf palatability of two native trees on bacterial colonization (abundance) over time, through a laboratory experiment that lasted 60 days. Values of C, N, P, C:N, C:P, polyphenols, tannins, lignin, lignin:N and leaf toughness of both species were compared. Bacterial abundance was higher in species with higher nitrogen values, although they had higher leaf toughness and more polyphenols, which differs from studies indicating that high leaf toughness represents low nutritional quality. The colonization time did not influence bacterial abundance. Therefore, processes degrading riparian vegetation and reducing nutritional quality can affect local decomposition, decreasing bacterial abundance.
{"title":"Effects of leaf quality and colonization time on the abundance of bacteria in an experimental design","authors":"Vinícius da Silva Rasvailer, Camila Gentilin-Avanci, M. Scoarize, G. D. Pinha, E. Benedito","doi":"10.4025/actascibiolsci.v44i1.61495","DOIUrl":"https://doi.org/10.4025/actascibiolsci.v44i1.61495","url":null,"abstract":"Small rivers, henceforth streams, depend on organic matter (nutrients and energy) from riparian vegetation. The quality of such allochthonous debris is determinant for the transformation of organic matter compounds, where the bacterial community has a crucial role in the final decomposition of the substrate. During bacterial colonization, debris with higher concentration of nutrients (more palatable) is prioritized, which accelerates the process. This study investigated the effects of leaf palatability of two native trees on bacterial colonization (abundance) over time, through a laboratory experiment that lasted 60 days. Values of C, N, P, C:N, C:P, polyphenols, tannins, lignin, lignin:N and leaf toughness of both species were compared. Bacterial abundance was higher in species with higher nitrogen values, although they had higher leaf toughness and more polyphenols, which differs from studies indicating that high leaf toughness represents low nutritional quality. The colonization time did not influence bacterial abundance. Therefore, processes degrading riparian vegetation and reducing nutritional quality can affect local decomposition, decreasing bacterial abundance.","PeriodicalId":7166,"journal":{"name":"Acta Scientiarum. Biological Sciences","volume":"38 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90676318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-28DOI: 10.4025/actascibiolsci.v44i1.58869
R. Silva, Gabrielly Terassi Bersaneti, B. G. Bigotto, V. A. I. Silveira, A. Lonni, D. Borsato, M. Celligoi
The addition of natural molecules such as microbial exopolysaccharides in cosmetics is a trend in the current market, adding properties and improving the product quality. Therefore, the aim of this study was to develop a facial biocosmetic formulation containing microbial levan, almond and cinnamon oils. The centroid-simplex design was used to evaluate the spreadability, antioxidant activity, moisture retention capacity and viscosity of formulations. Since it is a facial cosmetic, the formulation was optimized using the intermediate viscosity. The optimized formulation with intermediate viscosity was 75% (0.75 g) levan and 25% (2 mL) almond oil, without the addition of cinnamon oil. This formulation was submitted to 90 days under different exposure conditions, and the results showed a spreadability of 805 mm2, pH and density ideal for the facial area, with an antioxidant activity of 72%, hydration capacity of 100.3%, viscosity with no-Newtonian behavior, and normal organoleptic properties when stored at room and low temperature. The formulation with levan associated with almond oil showed potential for application in the facial area, with high antioxidant properties, moisturizing intermediate viscosity and stability for 90 days. The utilization of centroid-simplex design allowed the development of a biocosmetic with desired characteristics just by adjusting the concentrations of the bioactive.
{"title":"Development of a facial biocosmetic containing levan, almond and cinnamon oils with antioxidant and moisturizing properties","authors":"R. Silva, Gabrielly Terassi Bersaneti, B. G. Bigotto, V. A. I. Silveira, A. Lonni, D. Borsato, M. Celligoi","doi":"10.4025/actascibiolsci.v44i1.58869","DOIUrl":"https://doi.org/10.4025/actascibiolsci.v44i1.58869","url":null,"abstract":"The addition of natural molecules such as microbial exopolysaccharides in cosmetics is a trend in the current market, adding properties and improving the product quality. Therefore, the aim of this study was to develop a facial biocosmetic formulation containing microbial levan, almond and cinnamon oils. The centroid-simplex design was used to evaluate the spreadability, antioxidant activity, moisture retention capacity and viscosity of formulations. Since it is a facial cosmetic, the formulation was optimized using the intermediate viscosity. The optimized formulation with intermediate viscosity was 75% (0.75 g) levan and 25% (2 mL) almond oil, without the addition of cinnamon oil. This formulation was submitted to 90 days under different exposure conditions, and the results showed a spreadability of 805 mm2, pH and density ideal for the facial area, with an antioxidant activity of 72%, hydration capacity of 100.3%, viscosity with no-Newtonian behavior, and normal organoleptic properties when stored at room and low temperature. The formulation with levan associated with almond oil showed potential for application in the facial area, with high antioxidant properties, moisturizing intermediate viscosity and stability for 90 days. The utilization of centroid-simplex design allowed the development of a biocosmetic with desired characteristics just by adjusting the concentrations of the bioactive.","PeriodicalId":7166,"journal":{"name":"Acta Scientiarum. Biological Sciences","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87223143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-28DOI: 10.4025/actascibiolsci.v44i1.61925
Daniel Leirias Caurio, Danieli Quadros da Silva, L. Dornelles, Nathasha Noronha Arechavaleta, A. S. Mota
In the food industry, the formation of biofilms results in serious microbial recontamination problem. This work aimed to study Listeria sp. obtained from sliced products and food handling surfaces, for the preparation of food of animal origin to be consumed cold. Listeria monocytogenes, Listeria innocua and Listeria seeligeri were detected. In the evaluation of antibiotic susceptibility, the isolates were sensitive to ampicillin and penicillin, which were the main antibiotics used in treatment. The isolates showed the ability to form biofilm in microplate, in stainless steel and polypropylene surfaces, with a variation of sessile cells between 4.29±0.23 and 7.03±0.01 log10 CFU cm-2 in TSBYE. This ability was also observed in food matrix composed of UHT whole milk in mono-species cultivation and in associated cultivation of L. monocytogenes with Pseudomonas fluorescens. The application of sanitizers peracetic acid and sodium hypochlorite revealed efficiency in the eradication of adhered cells and biofilms formed in stainless steel surfaces. Therefore, Listeria sp. showed to be persistent and able to form biofilm in mono-species cultivation or associated with P. fluorescens, under different conditions. Taking these aspects into account, the need for proper hygiene in the food production process is highlighted in order to avoid risks to the consumers health
{"title":"Influence of the food matrix on dual-species biofilm of Listeria monocytogenes and Pseudomonas fluorescens","authors":"Daniel Leirias Caurio, Danieli Quadros da Silva, L. Dornelles, Nathasha Noronha Arechavaleta, A. S. Mota","doi":"10.4025/actascibiolsci.v44i1.61925","DOIUrl":"https://doi.org/10.4025/actascibiolsci.v44i1.61925","url":null,"abstract":"In the food industry, the formation of biofilms results in serious microbial recontamination problem. This work aimed to study Listeria sp. obtained from sliced products and food handling surfaces, for the preparation of food of animal origin to be consumed cold. Listeria monocytogenes, Listeria innocua and Listeria seeligeri were detected. In the evaluation of antibiotic susceptibility, the isolates were sensitive to ampicillin and penicillin, which were the main antibiotics used in treatment. The isolates showed the ability to form biofilm in microplate, in stainless steel and polypropylene surfaces, with a variation of sessile cells between 4.29±0.23 and 7.03±0.01 log10 CFU cm-2 in TSBYE. This ability was also observed in food matrix composed of UHT whole milk in mono-species cultivation and in associated cultivation of L. monocytogenes with Pseudomonas fluorescens. The application of sanitizers peracetic acid and sodium hypochlorite revealed efficiency in the eradication of adhered cells and biofilms formed in stainless steel surfaces. Therefore, Listeria sp. showed to be persistent and able to form biofilm in mono-species cultivation or associated with P. fluorescens, under different conditions. Taking these aspects into account, the need for proper hygiene in the food production process is highlighted in order to avoid risks to the consumers health","PeriodicalId":7166,"journal":{"name":"Acta Scientiarum. Biological Sciences","volume":"93 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90423607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-28DOI: 10.4025/actascibiolsci.v44i1.58300
Saray Paternina-Ricardo, Barbara Arroyo Salgado, Maria Cecilia García Espiñeira
Lepidium meyenii (maca) is a Peruvian nutraceutical plant, whose hypocotyl has a variety of colors ranging from black to white. The black and red varieties of maca have been the most studied since their extracts are associated with effects such as increased sperm count, decreased glucose levels, reversal of prostatic hyperplasia, among others. However, the properties related to reduction of oxidative stress, metabolic diseases and anti-aging have not yet been confirmed. The aim was to evaluate the effects of aqueous and ethanolic extracts, obtained from spray-dried hydroalcoholic extract of hypocotyl of black and red maca (Lepidium meyenii), on mortality, growth, reproduction, lipid accumulation and the expression levels of genes related to oxidative stress and heat shock, in the in-vivo model, Caenorhabditis elegans (C. elegans), using different concentrations. The results showed that maca extracts were not toxic to the model at concentrations below 100 mg L-1. However, higher concentrations caused high mortality, growth disturbances, oxidative stress and lipid accumulation. Black maca extracts increased the reproduction of C. elegans by increasing the number of offspring in C. elegans, both in aqueous and ethanolic extracts. On the other hand, ethanolic extracts produced an increase in the expression of genes related to oxidative stress, indicating a mild stressor behavior of the same. C. elegans represents an established model for evaluating the biological properties of nutraceutical plants of biological interest and can be used in the search for antioxidant activity of L. meyenii (hypocotyl), as well as it can be used in future studies to identify some metabolites involved in each biological property and to understand the biochemical and molecular mechanisms involved in these properties.
{"title":"Effects of aqueous and ethanolic extracts of Lepidium meyenii Walp (red and black maca) on Caenorhabditis elegans","authors":"Saray Paternina-Ricardo, Barbara Arroyo Salgado, Maria Cecilia García Espiñeira","doi":"10.4025/actascibiolsci.v44i1.58300","DOIUrl":"https://doi.org/10.4025/actascibiolsci.v44i1.58300","url":null,"abstract":"Lepidium meyenii (maca) is a Peruvian nutraceutical plant, whose hypocotyl has a variety of colors ranging from black to white. The black and red varieties of maca have been the most studied since their extracts are associated with effects such as increased sperm count, decreased glucose levels, reversal of prostatic hyperplasia, among others. However, the properties related to reduction of oxidative stress, metabolic diseases and anti-aging have not yet been confirmed. The aim was to evaluate the effects of aqueous and ethanolic extracts, obtained from spray-dried hydroalcoholic extract of hypocotyl of black and red maca (Lepidium meyenii), on mortality, growth, reproduction, lipid accumulation and the expression levels of genes related to oxidative stress and heat shock, in the in-vivo model, Caenorhabditis elegans (C. elegans), using different concentrations. The results showed that maca extracts were not toxic to the model at concentrations below 100 mg L-1. However, higher concentrations caused high mortality, growth disturbances, oxidative stress and lipid accumulation. Black maca extracts increased the reproduction of C. elegans by increasing the number of offspring in C. elegans, both in aqueous and ethanolic extracts. On the other hand, ethanolic extracts produced an increase in the expression of genes related to oxidative stress, indicating a mild stressor behavior of the same. C. elegans represents an established model for evaluating the biological properties of nutraceutical plants of biological interest and can be used in the search for antioxidant activity of L. meyenii (hypocotyl), as well as it can be used in future studies to identify some metabolites involved in each biological property and to understand the biochemical and molecular mechanisms involved in these properties.","PeriodicalId":7166,"journal":{"name":"Acta Scientiarum. Biological Sciences","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84863941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-28DOI: 10.4025/actascibiolsci.v44i1.62438
Fábio Antônio Antonelo, M. S. Rodrigues, Américo Wagner Júnior, P. F. Montanher
The biodiversity found in Brazilian’s ecosystems brings the possibility of discovering new natural products with wide application potentials. However, knowing their availability and chemical composition is crucial. Thus, the aim of this study was to evaluate the extraction yield, chemical composition and antioxidant activity of essential oil from fresh leaves of Calyptranthes concinna DC., a native species of Myrtaceae occurring in Brazilian Atlantic Rain Forest. Plant samples were collected in Southeastern Brazil and the essential oil was extracted by hydrodistillation. The chemical composition was evaluated by Gas Chromatography associated with Mass Spectrometry and antioxidant activity was measured using ABTS, DPPH and FRAP methods. The extraction yield obtained was 0.015% (v), and the chemical composition revealed elemicin, a phenylpropanoid as the major component (36.46%). Still, β-caryophyllene (16.94%), germacrene B (8.28%) and spathulenol (7.33%) proved to be relevant for the same essential oil. Antioxidant activity was obtained for ABTS and DPPH radical scavenge (134.82 ± 2,9 and 93.70 ± 1.7 µM TE mL-1, respectively) and FRAP (11.31 ± 0.2 µM FeSO4 mL-1 OE), revealing hydrogen-donation as the main antioxidant mechanism. To our knowledge, this is the first report of antioxidant activity of C. concinna essential oil. The product presented compounds of great relevance, with possibilities of application in different areas including food, agriculture and pharmaceutical segments
巴西生态系统的生物多样性为发现具有广泛应用潜力的新天然产物提供了可能。然而，了解它们的可用性和化学成分是至关重要的。因此，本研究的目的是评价calyptranths coninna DC鲜叶精油的提取率、化学成分和抗氧化活性。桃金娘科的一种，产于巴西大西洋雨林。在巴西东南部采集植物标本，采用加氢蒸馏法提取精油。采用气相色谱联用质谱法测定其化学成分，采用ABTS、DPPH和FRAP法测定其抗氧化活性。提取率为0.015% (v)，主要成分为苯丙素(36.46%)。然而，β-石竹烯(16.94%)、芽胞烯B(8.28%)和spathulenol(7.33%)被证明与同一种精油相关。对ABTS和DPPH自由基(分别为134.82±2、9和93.70±1.7µM TE mL-1)和FRAP(11.31±0.2µM FeSO4 mL-1 OE)的抗氧化活性，表明给氢是其主要的抗氧化机制。据我们所知，这是第一次报道芫花精油的抗氧化活性。该产品提供了非常相关的化合物，具有在包括食品，农业和制药部门在内的不同领域应用的可能性
{"title":"Chemical composition and antioxidant activity of leaf essential oil from Calyptranthes concinna DC. (Myrtaceae)","authors":"Fábio Antônio Antonelo, M. S. Rodrigues, Américo Wagner Júnior, P. F. Montanher","doi":"10.4025/actascibiolsci.v44i1.62438","DOIUrl":"https://doi.org/10.4025/actascibiolsci.v44i1.62438","url":null,"abstract":"The biodiversity found in Brazilian’s ecosystems brings the possibility of discovering new natural products with wide application potentials. However, knowing their availability and chemical composition is crucial. Thus, the aim of this study was to evaluate the extraction yield, chemical composition and antioxidant activity of essential oil from fresh leaves of Calyptranthes concinna DC., a native species of Myrtaceae occurring in Brazilian Atlantic Rain Forest. Plant samples were collected in Southeastern Brazil and the essential oil was extracted by hydrodistillation. The chemical composition was evaluated by Gas Chromatography associated with Mass Spectrometry and antioxidant activity was measured using ABTS, DPPH and FRAP methods. The extraction yield obtained was 0.015% (v), and the chemical composition revealed elemicin, a phenylpropanoid as the major component (36.46%). Still, β-caryophyllene (16.94%), germacrene B (8.28%) and spathulenol (7.33%) proved to be relevant for the same essential oil. Antioxidant activity was obtained for ABTS and DPPH radical scavenge (134.82 ± 2,9 and 93.70 ± 1.7 µM TE mL-1, respectively) and FRAP (11.31 ± 0.2 µM FeSO4 mL-1 OE), revealing hydrogen-donation as the main antioxidant mechanism. To our knowledge, this is the first report of antioxidant activity of C. concinna essential oil. The product presented compounds of great relevance, with possibilities of application in different areas including food, agriculture and pharmaceutical segments","PeriodicalId":7166,"journal":{"name":"Acta Scientiarum. Biological Sciences","volume":"132 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79637125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-28DOI: 10.4025/actascibiolsci.v44i1.60418
Juliana Elias de Oliveira, L. Dalvi, F. D. Oliveira, D. Pratissoli, G. Andrade, J. Pedrosa
Weed infestation is one of the main challenges in crop management. The environmental, economic and social impacts attributed to pesticides lead to the search for new sustainable possibilities for the management of these plants, especially for Commelina benghalensis, which is considered one of the worst weeds in the world. This work consists of a description of the external morphology of the insect Lema quadrivittata and its potential use as a biological regulator of C. benghalensis, a weed plant, which suffers effective injuries by this natural enemy. Plots with high infestation of C. benghalensis were inspected to find plant damage and its possible causes. Upon identifying the occurrence of L. quadrivittata, it was decided to cultivate C. benghalensis in a greenhouse in order to observe the interaction of the insect with the plant. In this sense, during the study, we identified the insect L. quadrivittata by describing its morphological structures of adults and larvae and its interaction with C. benghalensis as a biological control agent. This work is an approach to the attack of L. quadrivittata on C. benghalensis. Future studies are needed to indicate the potential of this insect as a biological regulator of this weed from the knowledge of its behavior, morphology and evaluations of potential hosts.
{"title":"Lema quadrivittata (Coleoptera: Chrysomelidae): morphological description and its use as a biological control agent of Commelina benghalensis L.","authors":"Juliana Elias de Oliveira, L. Dalvi, F. D. Oliveira, D. Pratissoli, G. Andrade, J. Pedrosa","doi":"10.4025/actascibiolsci.v44i1.60418","DOIUrl":"https://doi.org/10.4025/actascibiolsci.v44i1.60418","url":null,"abstract":"Weed infestation is one of the main challenges in crop management. The environmental, economic and social impacts attributed to pesticides lead to the search for new sustainable possibilities for the management of these plants, especially for Commelina benghalensis, which is considered one of the worst weeds in the world. This work consists of a description of the external morphology of the insect Lema quadrivittata and its potential use as a biological regulator of C. benghalensis, a weed plant, which suffers effective injuries by this natural enemy. Plots with high infestation of C. benghalensis were inspected to find plant damage and its possible causes. Upon identifying the occurrence of L. quadrivittata, it was decided to cultivate C. benghalensis in a greenhouse in order to observe the interaction of the insect with the plant. In this sense, during the study, we identified the insect L. quadrivittata by describing its morphological structures of adults and larvae and its interaction with C. benghalensis as a biological control agent. This work is an approach to the attack of L. quadrivittata on C. benghalensis. Future studies are needed to indicate the potential of this insect as a biological regulator of this weed from the knowledge of its behavior, morphology and evaluations of potential hosts.","PeriodicalId":7166,"journal":{"name":"Acta Scientiarum. Biological Sciences","volume":"36 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84427977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-28DOI: 10.4025/actascibiolsci.v44i1.62665
Marcos Vinícius Cosme, N. M. F. Nunes, A. P. Oliveira, Elisângela Cláudia Alves de Oliveira
The species Rosmarinus officinalis L. (rosemary) is an herb from the Lamiaceae family, widely used in cooking as a food preservative, seasoning or condiment. It also stands out for its therapeutic properties, mainly presenting antioxidant, antibacterial and antitumor activity. The aim of this study was to evaluate the cytotoxic and mutagenic activity of R. officinalis L. essential oil through the Allium cepa bioassay. This test constitutes an excellent plant model routinely used due to its sensitivity, low cost and good correlation with test systems in mammals. The defined concentrations for carrying out the test were 750, 243, 81 and 27 µg mL-1. Five bulbs were used, 4 roots of each, measuring approximately 2 cm, and they were analyzed on two slides. All assays were performed at least in triplicate and compared to the negative control. The statistical test of analysis of variance (ANOVA with a fixed factor) was used, followed by Tukey's multiple comparisons test, for p < 0.05. For this purpose, the GraphPad Prism program (version 6.0) was used. The results showed a cytotoxic and mutagenic effect for all concentrations used of the essential oil of R. officinalis L. However, it is important to conduct further research using other genotoxicological tests with different endpoints and at different concentrations, in order to clarify the interaction of the essential oil of the species R. officinalis L. with the genetic material of the cell and its possible mechanism of action.
迷迭香(Rosmarinus officinalis L.)是迷迭香科的一种草本植物，广泛用于烹饪中作为食品防腐剂、调味料或调味品。它的治疗特性也很突出，主要表现为抗氧化、抗菌和抗肿瘤活性。本研究的目的是通过对葱精油的生物测定，评价葱精油的细胞毒和诱变活性。由于其敏感性、低成本和与哺乳动物测试系统的良好相关性，该测试构成了一种优秀的常规植物模型。测定的浓度分别为750、243、81和27µg mL-1。使用5个球茎，每个4根，长约2厘米，在两张载玻片上分析。所有试验至少重复三次，并与阴性对照进行比较。采用方差分析统计检验(ANOVA with a fixed factor)，采用Tukey多重比较检验，p < 0.05。为此，使用GraphPad Prism程序(6.0版本)。结果表明，在不同浓度的黄颡鱼挥发油中，黄颡鱼挥发油均具有细胞毒性和致突变作用，但为了进一步阐明黄颡鱼挥发油与细胞遗传物质的相互作用及其可能的作用机制，还需要开展其他不同终点和不同浓度的基因毒理学试验。
{"title":"Evaluation of the cytotoxic and mutagenic potential of Rosmarinus officinalis L. essential oil through the Allium cepa Leach bioassay","authors":"Marcos Vinícius Cosme, N. M. F. Nunes, A. P. Oliveira, Elisângela Cláudia Alves de Oliveira","doi":"10.4025/actascibiolsci.v44i1.62665","DOIUrl":"https://doi.org/10.4025/actascibiolsci.v44i1.62665","url":null,"abstract":"The species Rosmarinus officinalis L. (rosemary) is an herb from the Lamiaceae family, widely used in cooking as a food preservative, seasoning or condiment. It also stands out for its therapeutic properties, mainly presenting antioxidant, antibacterial and antitumor activity. The aim of this study was to evaluate the cytotoxic and mutagenic activity of R. officinalis L. essential oil through the Allium cepa bioassay. This test constitutes an excellent plant model routinely used due to its sensitivity, low cost and good correlation with test systems in mammals. The defined concentrations for carrying out the test were 750, 243, 81 and 27 µg mL-1. Five bulbs were used, 4 roots of each, measuring approximately 2 cm, and they were analyzed on two slides. All assays were performed at least in triplicate and compared to the negative control. The statistical test of analysis of variance (ANOVA with a fixed factor) was used, followed by Tukey's multiple comparisons test, for p < 0.05. For this purpose, the GraphPad Prism program (version 6.0) was used. The results showed a cytotoxic and mutagenic effect for all concentrations used of the essential oil of R. officinalis L. However, it is important to conduct further research using other genotoxicological tests with different endpoints and at different concentrations, in order to clarify the interaction of the essential oil of the species R. officinalis L. with the genetic material of the cell and its possible mechanism of action.","PeriodicalId":7166,"journal":{"name":"Acta Scientiarum. Biological Sciences","volume":"33 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78965005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-28DOI: 10.4025/actascibiolsci.v44i1.62289
A. Adeleye, M. Yerima, Micheal Edem Nkereuwem, V. Onokebhagbe, G. P. Shiaka, A. O. Amoo, Catherine Iyabo Asaju, B. Yalwaji, Saheed Mohammed Ishaq
Bioenhancement of hydrocarbonoclastic microorganisms with suitable nutrients has a huge impact in achieving positive bioremediation of polluted environments. This study was conducted to assess the bio-enhancing effect of some organic amendments on Streptococcus pyogenes and Enterococcus faecalis co-culture with a view to remediating spent engine oil (SEO) contaminated soil. Top soil (1.5 kg) was autoclaved and thereafter contaminated with SEO at three levels. The contaminated soil was inoculated with bacterial co-culture (150 mL) and subsequently bioenhanced with compost, processed cocoa pod husk (CPH) and cow dung. The factorial experiment was laid out in completely randomized design. Concentrations of total petroleum hydrocarbon (TPH) and selected polycyclic aromatic hydrocarbons (PAH) were estimated on the first day, 5th week and 10th week of incubation. Results obtained show that bacterial co-culture bioenhanced with compost produced the most significant TPH reductions (1318 and 261 mg kg-1) on 10% SEO contaminated soil at the 5th and 10th week respectively (p<0.05). Again, bacterial co-culture bioenhanced with compost produced the most significant PAH reductions (65.9 and 55.8 mg kg-1) on 10% SEO contaminated soil at the 5th and 10th week respectively (p<0.05). The significant bioremediation capabilities exhibited by the bacterial co-culture bioenhanced with organic amendments in this study has made these bioremediation agents potential candidates in remediating soils impacted with petroleum hydrocarbons.
{"title":"Effect of bacterial co-culture and organic amendments on the bioremediation of hydrocarbons in a soil contaminated with spent engine oil","authors":"A. Adeleye, M. Yerima, Micheal Edem Nkereuwem, V. Onokebhagbe, G. P. Shiaka, A. O. Amoo, Catherine Iyabo Asaju, B. Yalwaji, Saheed Mohammed Ishaq","doi":"10.4025/actascibiolsci.v44i1.62289","DOIUrl":"https://doi.org/10.4025/actascibiolsci.v44i1.62289","url":null,"abstract":"Bioenhancement of hydrocarbonoclastic microorganisms with suitable nutrients has a huge impact in achieving positive bioremediation of polluted environments. This study was conducted to assess the bio-enhancing effect of some organic amendments on Streptococcus pyogenes and Enterococcus faecalis co-culture with a view to remediating spent engine oil (SEO) contaminated soil. Top soil (1.5 kg) was autoclaved and thereafter contaminated with SEO at three levels. The contaminated soil was inoculated with bacterial co-culture (150 mL) and subsequently bioenhanced with compost, processed cocoa pod husk (CPH) and cow dung. The factorial experiment was laid out in completely randomized design. Concentrations of total petroleum hydrocarbon (TPH) and selected polycyclic aromatic hydrocarbons (PAH) were estimated on the first day, 5th week and 10th week of incubation. Results obtained show that bacterial co-culture bioenhanced with compost produced the most significant TPH reductions (1318 and 261 mg kg-1) on 10% SEO contaminated soil at the 5th and 10th week respectively (p<0.05). Again, bacterial co-culture bioenhanced with compost produced the most significant PAH reductions (65.9 and 55.8 mg kg-1) on 10% SEO contaminated soil at the 5th and 10th week respectively (p<0.05). The significant bioremediation capabilities exhibited by the bacterial co-culture bioenhanced with organic amendments in this study has made these bioremediation agents potential candidates in remediating soils impacted with petroleum hydrocarbons.","PeriodicalId":7166,"journal":{"name":"Acta Scientiarum. Biological Sciences","volume":"47 10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82187499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-28DOI: 10.4025/actascibiolsci.v44i1.58294
R. C. P. Faquim, Ramilla dos Santos Braga Ferreira, T. G. Castro, Leonel Silva, M. Telles
Species Cichla piquiti and Cichla kelberi are found in the Serra da Mesa reservoir, Goiás and are sedentary with diurnal habits. This study aimed to evaluate the magnitude and distribution of genetic variability in subpopulations of C. piquiti with specific microsatellite loci and to test transferability in other microsatellite markers for C. kelberi. We analyzed 99 individuals of C. piquiti from seven points to evaluate genetic diversity and structure with 10 microsatellite loci. Transferability of 75 loci was tested in C. kelberi to increase microsatellite markers available. Genetic structure was assessed with Bayesian clustering. Global FST for C. piquiti was weak (0.056), but FIS (0.598) and FIT (0.621) were significantly high, indicating that the mating system has a strong influence on the organization of genetic variability with most mating among related. Two genetic groups were evidenced with most individuals allocated to a single group. Transferability of microsatellite loci for C. kelberi had low polymorphism. The level of genetic diversity was low, increasing inbreeding and suggesting that few individuals of C. piquiti colonized the reservoir during its installation due founder effect. Other factors as reproductive behavior and overfishing can act to decrease genetic diversity. Therefore, we reinforce the need for genetic monitoring to avoid loss of genetic diversity that can be intensified both construction of hydropower plants and ecological and reproductive aspects in some fish species.
物种Cichla piquiti和Cichla kelberi发现于Serra da Mesa水库Goiás，它们是久坐的，有白天的习性。本研究旨在评估具有特定微卫星位点的C. piquiti亚群遗传变异的大小和分布，并测试C. kelberi在其他微卫星标记上的可转移性。利用10个微卫星位点，从7个点分析了99个皮柑个体的遗传多样性和结构。为增加微卫星标记的可用性，对75个位点进行了可转移性检测。遗传结构采用贝叶斯聚类分析。全球FST较弱(0.056)，但FIS(0.598)和FIT(0.621)均显著较高，表明交配系统对遗传变异的组织有较强的影响，亲缘关系中的交配居多。两个遗传组被证实，大多数个体被分配到一个组。kelberi微卫星位点的可转移性具有低多态性。遗传多样性水平较低，近交增加，表明在水库安装过程中，由于奠基者效应，很少有皮皮提的个体在水库中定居。生殖行为和过度捕捞等其他因素也会减少遗传多样性。因此，我们强调有必要进行遗传监测，以避免遗传多样性的丧失，这些遗传多样性可以在水电站建设和某些鱼类的生态和生殖方面得到加强。
{"title":"Genetic diversity in Cichla piquiti and cross-amplification for Cichla kelberi in the Serra da Mesa reservoir, Goiás, Brazil","authors":"R. C. P. Faquim, Ramilla dos Santos Braga Ferreira, T. G. Castro, Leonel Silva, M. Telles","doi":"10.4025/actascibiolsci.v44i1.58294","DOIUrl":"https://doi.org/10.4025/actascibiolsci.v44i1.58294","url":null,"abstract":"Species Cichla piquiti and Cichla kelberi are found in the Serra da Mesa reservoir, Goiás and are sedentary with diurnal habits. This study aimed to evaluate the magnitude and distribution of genetic variability in subpopulations of C. piquiti with specific microsatellite loci and to test transferability in other microsatellite markers for C. kelberi. We analyzed 99 individuals of C. piquiti from seven points to evaluate genetic diversity and structure with 10 microsatellite loci. Transferability of 75 loci was tested in C. kelberi to increase microsatellite markers available. Genetic structure was assessed with Bayesian clustering. Global FST for C. piquiti was weak (0.056), but FIS (0.598) and FIT (0.621) were significantly high, indicating that the mating system has a strong influence on the organization of genetic variability with most mating among related. Two genetic groups were evidenced with most individuals allocated to a single group. Transferability of microsatellite loci for C. kelberi had low polymorphism. The level of genetic diversity was low, increasing inbreeding and suggesting that few individuals of C. piquiti colonized the reservoir during its installation due founder effect. Other factors as reproductive behavior and overfishing can act to decrease genetic diversity. Therefore, we reinforce the need for genetic monitoring to avoid loss of genetic diversity that can be intensified both construction of hydropower plants and ecological and reproductive aspects in some fish species.","PeriodicalId":7166,"journal":{"name":"Acta Scientiarum. Biological Sciences","volume":"93 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84489670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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