Pub Date : 2023-06-15DOI: 10.4025/actascibiolsci.v45i1.67277
Emanoelli Aparecida Rodrigues dos Santos, L. E. Tadielo, Thiago Henrique Bellé, Jhennifer Arruda Schmiedt, V. H. C. Dias, Paulo Henrique Silva Orisio, C. K. Cerqueira-Cézar, J. G. Pereira, V. C. Barcellos, L. Bersot
The objective of this study was to evaluate the activity of Rosmarinus officinalis essential oil (EO) compared to peracetic acid (PA) regarding formation and elimination of Listeria monocytogenes biofilms on polystyrene surface. The minimum inhibitory concentration (MIC) was determined according to standard protocol. Isolates were inoculated according to MIC standards polystyrene plate wells, which were then incubated at 37°C/96 hours for evaluation of biofilm formation. Regarding the evaluation of biofilm elimination, the biofilms were treated under MIC for 10 minutes. The MIC obtained were 2.0 and 3.0 mg mL-1 for EO and 0,015% for PA. Therefore, the results showed a reduction in the formation of biofilm with the presence of EO and PA, EO being more efficient (p < 0.05). Both compounds had a good capacity of eliminating biofilms, however the EO reduced the biofilm formation when compared to PA, highlighting its potential as an antibacterial agent and antibiofilm.
{"title":"Activity of rosemary (Rosmarinus officinalis - Family: Lamiaceae) essential oil compared to peracetic acid in Listeria monocytogenes biofilms","authors":"Emanoelli Aparecida Rodrigues dos Santos, L. E. Tadielo, Thiago Henrique Bellé, Jhennifer Arruda Schmiedt, V. H. C. Dias, Paulo Henrique Silva Orisio, C. K. Cerqueira-Cézar, J. G. Pereira, V. C. Barcellos, L. Bersot","doi":"10.4025/actascibiolsci.v45i1.67277","DOIUrl":"https://doi.org/10.4025/actascibiolsci.v45i1.67277","url":null,"abstract":"The objective of this study was to evaluate the activity of Rosmarinus officinalis essential oil (EO) compared to peracetic acid (PA) regarding formation and elimination of Listeria monocytogenes biofilms on polystyrene surface. The minimum inhibitory concentration (MIC) was determined according to standard protocol. Isolates were inoculated according to MIC standards polystyrene plate wells, which were then incubated at 37°C/96 hours for evaluation of biofilm formation. Regarding the evaluation of biofilm elimination, the biofilms were treated under MIC for 10 minutes. The MIC obtained were 2.0 and 3.0 mg mL-1 for EO and 0,015% for PA. Therefore, the results showed a reduction in the formation of biofilm with the presence of EO and PA, EO being more efficient (p < 0.05). Both compounds had a good capacity of eliminating biofilms, however the EO reduced the biofilm formation when compared to PA, highlighting its potential as an antibacterial agent and antibiofilm.","PeriodicalId":7166,"journal":{"name":"Acta Scientiarum. Biological Sciences","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89600712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-15DOI: 10.4025/actascibiolsci.v45i1.62761
Matheus Pena Campos, J. Rufini, B. M. Silva, M. Guedes, Y. M. García, Janaina Canaan Rezende de Souza, R. P. Azevedo, Miriã Cristina Pereira Fagundes
Water stress can affect all aspects of plant growth and development, compromising its productive potential. The cultivation of fruit species native to the Cerrado and the study of their behavior under conditions of low water availability are necessary activities, in view of their socioeconomic and environmental potential. This study was conducted to evaluate the effect of water deficit on growth and biomass production of the cagaita tree (Eugenia dysenterica DC.), a species endemic to the Cerrado (Brazilian Savannah). The experiment was performed in a greenhouse for 120 days in a randomized block design (RBD), with a double factorial arrangement (2x5), corresponding to two cagaita mother plants and five water conditions. We evaluated the growth and leaf, stem and root functional characteristics. The most severe water deficit conditions reduced shoot growth, number of leaves, production of biomass and dry matter and increased the specific and per plant mass root length of cagaita plants. Deficit extension had negative effects on its growth and development. There was a statistical difference between mother plants, with mother plant 2 being more resistant to drought. The application of a severe or longer water deficit affected shoot growth and reduced the appearance of new leaves. The stress caused by water deficiency allowed an increase in root functional characteristics, supporting the hypothesis that plants under adverse conditions focus on higher biomass production and convert a higher amount of dry matter into the roots. Leaf area did not show to be a functional characteristic, explaining the stress effects in E. dysenterica DC plants.
{"title":"Biometric and morphological adjustments of Eugenia. dysenterica DC. seedlings in response to increased soil water deficit","authors":"Matheus Pena Campos, J. Rufini, B. M. Silva, M. Guedes, Y. M. García, Janaina Canaan Rezende de Souza, R. P. Azevedo, Miriã Cristina Pereira Fagundes","doi":"10.4025/actascibiolsci.v45i1.62761","DOIUrl":"https://doi.org/10.4025/actascibiolsci.v45i1.62761","url":null,"abstract":"Water stress can affect all aspects of plant growth and development, compromising its productive potential. The cultivation of fruit species native to the Cerrado and the study of their behavior under conditions of low water availability are necessary activities, in view of their socioeconomic and environmental potential. This study was conducted to evaluate the effect of water deficit on growth and biomass production of the cagaita tree (Eugenia dysenterica DC.), a species endemic to the Cerrado (Brazilian Savannah). The experiment was performed in a greenhouse for 120 days in a randomized block design (RBD), with a double factorial arrangement (2x5), corresponding to two cagaita mother plants and five water conditions. We evaluated the growth and leaf, stem and root functional characteristics. The most severe water deficit conditions reduced shoot growth, number of leaves, production of biomass and dry matter and increased the specific and per plant mass root length of cagaita plants. Deficit extension had negative effects on its growth and development. There was a statistical difference between mother plants, with mother plant 2 being more resistant to drought. The application of a severe or longer water deficit affected shoot growth and reduced the appearance of new leaves. The stress caused by water deficiency allowed an increase in root functional characteristics, supporting the hypothesis that plants under adverse conditions focus on higher biomass production and convert a higher amount of dry matter into the roots. Leaf area did not show to be a functional characteristic, explaining the stress effects in E. dysenterica DC plants.","PeriodicalId":7166,"journal":{"name":"Acta Scientiarum. Biological Sciences","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74009168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-15DOI: 10.4025/actascibiolsci.v45i1.64163
B. D. Novello, Marcela Maria Zanatta, A. P. Albrecht, A. Barroso
The objective of this review is to bring information about innovations and technologies that, through genetic improvement, are being used to improve the sustainability and productivity of agricultural crops, improve human nutrition, as well as conservation and decontamination of soils. Bioremediation consists of using microorganisms that have the ability to modify or decompose certain pollutants, with the possibility of increasing their activity through genetic engineering, building new strains for the transformation of pollutants into inert substances. Genetic improvement is seeking to develop cultivars that are more tolerant to periods of water deficit. Plant biofortification consists of varieties of improved plants that have a higher content of vitamins and minerals, which are obtained through genetic improvement. Thus, biotechnology is once again essential for world agricultural production and can bring a series of other benefits to society.
{"title":"Bioremediation, drought tolerance and biofortification in biotechnological uses","authors":"B. D. Novello, Marcela Maria Zanatta, A. P. Albrecht, A. Barroso","doi":"10.4025/actascibiolsci.v45i1.64163","DOIUrl":"https://doi.org/10.4025/actascibiolsci.v45i1.64163","url":null,"abstract":"The objective of this review is to bring information about innovations and technologies that, through genetic improvement, are being used to improve the sustainability and productivity of agricultural crops, improve human nutrition, as well as conservation and decontamination of soils. Bioremediation consists of using microorganisms that have the ability to modify or decompose certain pollutants, with the possibility of increasing their activity through genetic engineering, building new strains for the transformation of pollutants into inert substances. Genetic improvement is seeking to develop cultivars that are more tolerant to periods of water deficit. Plant biofortification consists of varieties of improved plants that have a higher content of vitamins and minerals, which are obtained through genetic improvement. Thus, biotechnology is once again essential for world agricultural production and can bring a series of other benefits to society.","PeriodicalId":7166,"journal":{"name":"Acta Scientiarum. Biological Sciences","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78548487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-15DOI: 10.4025/actascibiolsci.v45i1.62090
G. Giacomini, G. F. Nachtigal, C. Martins, Adriane Roedel Hirdes, R. A. Valgas, A. Santos
Industrial processing of pecan nuts results in edible oil – with fractions of fatty acids, polyphenols and phytosterols - that may be used for managing phytopathogens. Besides, chitosan may interfere with pathogen development directly and/or activate mechanisms of defense in plant tissues. This study aimed at developing a novel, natural and eco-friendly fungicide based on pecan nut oil immobilized in chitosan and at evaluating its activity against Colletotrichum gloeosporioides, an agent of anthracnose in guava trees and other fruit trees. Changes in the immobilized material exhibited at bands were identified by infrared spectroscopy. Additionally, micrographs by scanning electron microscopy (SEM) showed efficient changes in the immobilized material, by comparison with the polymer chitosan. Decreases in Colletotrichum gloeosporioides sporulation in vitro were 37% in the case of pecan nut oil immobilized in chitosan (IO) and 39% in the case of non-immobilized pecan nut oil (NIO). These results are positive because this fungus produces a large number of spores which may disseminate and survive on surfaces of plant tissues infected by anthracnose. Immobilization of pecan oil in chitosan showed benefits in the synthesis of a novel, stable and eco-friendly material which may be applied to guava trees to control anthracnose.
{"title":"Eco-friendly fungicide based on chitosan and pecan nut oil: development and evaluation in anthracnose control","authors":"G. Giacomini, G. F. Nachtigal, C. Martins, Adriane Roedel Hirdes, R. A. Valgas, A. Santos","doi":"10.4025/actascibiolsci.v45i1.62090","DOIUrl":"https://doi.org/10.4025/actascibiolsci.v45i1.62090","url":null,"abstract":"Industrial processing of pecan nuts results in edible oil – with fractions of fatty acids, polyphenols and phytosterols - that may be used for managing phytopathogens. Besides, chitosan may interfere with pathogen development directly and/or activate mechanisms of defense in plant tissues. This study aimed at developing a novel, natural and eco-friendly fungicide based on pecan nut oil immobilized in chitosan and at evaluating its activity against Colletotrichum gloeosporioides, an agent of anthracnose in guava trees and other fruit trees. Changes in the immobilized material exhibited at bands were identified by infrared spectroscopy. Additionally, micrographs by scanning electron microscopy (SEM) showed efficient changes in the immobilized material, by comparison with the polymer chitosan. Decreases in Colletotrichum gloeosporioides sporulation in vitro were 37% in the case of pecan nut oil immobilized in chitosan (IO) and 39% in the case of non-immobilized pecan nut oil (NIO). These results are positive because this fungus produces a large number of spores which may disseminate and survive on surfaces of plant tissues infected by anthracnose. Immobilization of pecan oil in chitosan showed benefits in the synthesis of a novel, stable and eco-friendly material which may be applied to guava trees to control anthracnose.","PeriodicalId":7166,"journal":{"name":"Acta Scientiarum. Biological Sciences","volume":"43 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80053903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-25DOI: 10.4025/actascibiolsci.v45i1.58024
L. L. C. Corrêa, D. E. Silva, Phamela Katharina Müller, M. Petry
Specific techniques for the efficient capture of forest tinamous in a short sampling time are yet poorly described in the literature. Here, we report the efficiency of the bell-trap in the capture of Brown Tinamou (Crypturellus obsoletus) and Tataupa Tinamou (Crypturellus tataupa), in southern Brazil. We used three bell-traps adapted with casting nets. In 80 hours of sampling effort, four adult individuals of C. obsoletus were captured, in a forest area in the Pampa Biome. This study area is located in the municipality of São Sepé, state of Rio Grande do Sul, Brazil. Subsequently, in 16 hours of sampling effort in a forest area of the Atlantic Forest domain, one individual of C. obsoletus and one of C. tataupa were captured. This other study area is located in the municipality of Passo do Sobrado, RS. The bell-trap method has already been reported as efficient in capturing Yellow-legged Tinamous (Crypturellus noctivagus), in southern Brazil. However, since forest tinamous are cryptical birds, field adaptations are often necessary to achieve success in sampling activities. The use of this method proved efficient in the capture of forest tinamous, generating an important tool for the collection of bioecological data of this group of birds, which still represents a lack of studies.
在较短的采样时间内有效捕获森林褐飞虫的具体技术在文献中还没有得到很好的描述。在此，我们报告了钟形诱捕器捕获巴西南部褐田鼠(Crypturellus obsoletus)和Tataupa田鼠(Crypturellus Tataupa)的效率。我们用了三个装有渔网的捕钟器。在Pampa Biome的一个森林区域，在80小时的采样努力中，捕获了4只成年的C. obsoletus。本研究区位于巴西南里奥格兰德州的s o sese市。随后，在大西洋森林域的一个森林区域进行了16小时的采样工作，捕获了1只C. obsoletus和1只C. tataupa。另一个研究区域位于巴西索布拉多州的Passo do Sobrado市。据报道，在巴西南部，钟形陷阱法在捕获黄腿夜莺(Crypturellus noctivagus)方面是有效的。然而，由于森林天鸟是神秘的鸟类，野外适应往往是取得成功的采样活动所必需的。事实证明，该方法在捕获森林天鸟中是有效的，为收集这类鸟类的生物生态数据提供了重要的工具，这一研究仍然缺乏。
{"title":"Bell-trap effectiveness to capture Crypturellus obsoletus and Crypturellus tataupa (Aves: Tinamidae) in southern Brazil","authors":"L. L. C. Corrêa, D. E. Silva, Phamela Katharina Müller, M. Petry","doi":"10.4025/actascibiolsci.v45i1.58024","DOIUrl":"https://doi.org/10.4025/actascibiolsci.v45i1.58024","url":null,"abstract":"Specific techniques for the efficient capture of forest tinamous in a short sampling time are yet poorly described in the literature. Here, we report the efficiency of the bell-trap in the capture of Brown Tinamou (Crypturellus obsoletus) and Tataupa Tinamou (Crypturellus tataupa), in southern Brazil. We used three bell-traps adapted with casting nets. In 80 hours of sampling effort, four adult individuals of C. obsoletus were captured, in a forest area in the Pampa Biome. This study area is located in the municipality of São Sepé, state of Rio Grande do Sul, Brazil. Subsequently, in 16 hours of sampling effort in a forest area of the Atlantic Forest domain, one individual of C. obsoletus and one of C. tataupa were captured. This other study area is located in the municipality of Passo do Sobrado, RS. The bell-trap method has already been reported as efficient in capturing Yellow-legged Tinamous (Crypturellus noctivagus), in southern Brazil. However, since forest tinamous are cryptical birds, field adaptations are often necessary to achieve success in sampling activities. The use of this method proved efficient in the capture of forest tinamous, generating an important tool for the collection of bioecological data of this group of birds, which still represents a lack of studies.","PeriodicalId":7166,"journal":{"name":"Acta Scientiarum. Biological Sciences","volume":"25 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87192096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-19DOI: 10.4025/actascibiolsci.v45i1.63169
Ivan Becari-Viana, P. Schwartsburd, A. A. Azevedo
Intercellular protuberances (IPs) are projections on the cell surface and have been reported for families of ferns, Gymnosperms, and Angiosperms. Data on the type, chemical composition, and distribution of these structures among vascular plants are still scarce. Here, we investigate the occurrence, distribution, type of IPs among species of eight Dennstaedtiaceae genera and verify the taxonomic significance of these protuberances in the family. Rhizomes of 23 species of Dennstaedtiaceae from field and herbaria collections were analyzed by light microscopy and scanning electron microscopy. Histochemical tests were performed to identify the main classes of IPs chemical compounds. Two types of IPs were observed in Dennstaedtiaceae species: strand and filament types. They were observed in the intercellular spaces of the parenchyma in the cortex and pith regions. Overall, protuberances are irregularly shaped, with angustate or spheroidal apices. Their polysaccharide nature and pectic constitution were confirmed by histochemical tests. Concerning Pteridium arachnoideum (Kaulf.) Maxon subsp. arachnoideum, IPs have confirmed phenolic composition. Evidence indicates that IPs in Dennstaedtiaceae originate from the fragmentation of the middle lamella and that they have a structural function as well as protection against pathogens. In lateral-line aerenchyma, the occurrence of filament-type IPs may be related to the larger intercellular spacing in the cortex region, providing greater mechanical resistance. We have expanded the data on the occurrence of IPs in the Dennstaedtiaceae, which appear to be notable characters for the family. Moreover, the data presented herein confirmed the polysaccharide and pectic nature of these structures. However, we were unable to find links between IPs and taxonomy and evolution of the Dennstaedtiaceae. On the other hand, different IPs types were identified between the clades Dennstaedtioideae (strand-type IPs) and Hypolepidoideae (filament-type IPs, with exceptions).
{"title":"Structure and chemical nature of intercellular protuberances in Dennstaedtiaceae rhizomes (Polypodiopsida)","authors":"Ivan Becari-Viana, P. Schwartsburd, A. A. Azevedo","doi":"10.4025/actascibiolsci.v45i1.63169","DOIUrl":"https://doi.org/10.4025/actascibiolsci.v45i1.63169","url":null,"abstract":"Intercellular protuberances (IPs) are projections on the cell surface and have been reported for families of ferns, Gymnosperms, and Angiosperms. Data on the type, chemical composition, and distribution of these structures among vascular plants are still scarce. Here, we investigate the occurrence, distribution, type of IPs among species of eight Dennstaedtiaceae genera and verify the taxonomic significance of these protuberances in the family. Rhizomes of 23 species of Dennstaedtiaceae from field and herbaria collections were analyzed by light microscopy and scanning electron microscopy. Histochemical tests were performed to identify the main classes of IPs chemical compounds. Two types of IPs were observed in Dennstaedtiaceae species: strand and filament types. They were observed in the intercellular spaces of the parenchyma in the cortex and pith regions. Overall, protuberances are irregularly shaped, with angustate or spheroidal apices. Their polysaccharide nature and pectic constitution were confirmed by histochemical tests. Concerning Pteridium arachnoideum (Kaulf.) Maxon subsp. arachnoideum, IPs have confirmed phenolic composition. Evidence indicates that IPs in Dennstaedtiaceae originate from the fragmentation of the middle lamella and that they have a structural function as well as protection against pathogens. In lateral-line aerenchyma, the occurrence of filament-type IPs may be related to the larger intercellular spacing in the cortex region, providing greater mechanical resistance. We have expanded the data on the occurrence of IPs in the Dennstaedtiaceae, which appear to be notable characters for the family. Moreover, the data presented herein confirmed the polysaccharide and pectic nature of these structures. However, we were unable to find links between IPs and taxonomy and evolution of the Dennstaedtiaceae. On the other hand, different IPs types were identified between the clades Dennstaedtioideae (strand-type IPs) and Hypolepidoideae (filament-type IPs, with exceptions).","PeriodicalId":7166,"journal":{"name":"Acta Scientiarum. Biological Sciences","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91282642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-19DOI: 10.4025/actascibiolsci.v45i1.63434
T. P. Gouvêa, G. Stefani-Santos, D. Vilela, W. F. Á. Ávila Júnior, Marcos Magalhães de Souza
Faunal inventories are essential for biota management and conservation, especially in areas with potential for the creation of conservation units. Inventories of insect taxa such as dragonflies and damselflies (Odonata), which perform several environmental services in aquatic and terrestrial ecosystems, are of great importance. In view of the above, this study aimed to update and expand the list of Odonata species in the Barroso region, Minas Gerais State, Brazil. This study was carried out in three areas of forest fragments in Atlantic Forest and Cerrado biomes in October 2020, December 2020, January 2021, and March 2021. The sampling effort was 8 hours per day during 20 days, totaling 140 sampling hours. A total of 43 Odonata species were recorded, which increased the richness of the study area from 57 to 76 species. The studied areas harbor rare and endangered species. However, since the last sampling in 2009, there has been a significant reduction in diversity in the Atlantic Forest fragment. In view of the changes that forest fragments are undergoing, we underscore the need to create a conservation unit, especially in Baú Forest.
{"title":"Odonata community in transition areas between Cerrado and Atlantic Forest biomes in south-central Minas Gerais, Brazil","authors":"T. P. Gouvêa, G. Stefani-Santos, D. Vilela, W. F. Á. Ávila Júnior, Marcos Magalhães de Souza","doi":"10.4025/actascibiolsci.v45i1.63434","DOIUrl":"https://doi.org/10.4025/actascibiolsci.v45i1.63434","url":null,"abstract":"Faunal inventories are essential for biota management and conservation, especially in areas with potential for the creation of conservation units. Inventories of insect taxa such as dragonflies and damselflies (Odonata), which perform several environmental services in aquatic and terrestrial ecosystems, are of great importance. In view of the above, this study aimed to update and expand the list of Odonata species in the Barroso region, Minas Gerais State, Brazil. This study was carried out in three areas of forest fragments in Atlantic Forest and Cerrado biomes in October 2020, December 2020, January 2021, and March 2021. The sampling effort was 8 hours per day during 20 days, totaling 140 sampling hours. A total of 43 Odonata species were recorded, which increased the richness of the study area from 57 to 76 species. The studied areas harbor rare and endangered species. However, since the last sampling in 2009, there has been a significant reduction in diversity in the Atlantic Forest fragment. In view of the changes that forest fragments are undergoing, we underscore the need to create a conservation unit, especially in Baú Forest.","PeriodicalId":7166,"journal":{"name":"Acta Scientiarum. Biological Sciences","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79823914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-19DOI: 10.4025/actascibiolsci.v45i1.63722
Stefhani Cristina Chan Jacik, J. Aquino, L. Melo, R. Rodrigues, A. Melo, M. Miglino, R. Rici
The family Potamotrygonidae are the only species of stingrays restricted to fresh water and located exclusively in South America. The objective of this research was to analyze the morphological aspects and germ cells of the male reproductive tract of Potamotrygon amandae. The samples were fixed in 10% formalin, and then dehydrated in an ascending ethanol series (70 to 100%). To carry out light microscopy analyses, they were embedded in paraffin, cut and stained; as for scanning electron microscopy analyses, the samples were dried, glued in metallic bases and metalized. The gross morphology consisted of the following paired organs: testis, epididymis, deferent duct, Leydig gland, seminal vesicle, clasper, and the clasper gland. Microscopically, several stages of spermatogenesis were observed in the testis, occurring in spherical follicles, similar to other stingrays. The epididymis was formed by one duct subdivided in various tubules. The deferent ducts were continuous with the epididymis, and the lumen was full of spermatozoa. The Leydig glands consisted of glandular units with eosinophilic content in the lumen of some, and the deferent ducts ran parallel to the ventral portion. The seminal vesicles possessed numerous compartments to store the sperm, with a wall similar to a hive, and the lumen was full of spermatozoa. Alcian Blue (AB) and Periodic Schiff-Acid (PAS) performed in the Leydig Gland, deferens ducts and seminal vesicle was positive only in the connective tissue, the cilia were PAS+ and the nuclei stained weakly for AB. The clasper gland was composed of unit glands and was covered with striated muscle externally. It stained very well with Periodic Schiff-Acid. The morphological aspects of the male reproductive tract of Potamotrygon amandae were similar to other stingrays.
黄貂鱼科是唯一一种只生活在淡水中的黄貂鱼，而且只生活在南美洲。本研究的目的是分析雄性金刚蝠生殖道的形态特征和生殖细胞。样品在10%的福尔马林中固定，然后在上升的乙醇系列(70 - 100%)中脱水。为了进行光镜分析，将它们包埋在石蜡中，切割和染色;扫描电镜分析，样品干燥，粘在金属基和金属化。大体形态由以下成对器官组成:睾丸、附睾、异管、间质腺、精囊、钩腺和钩腺。显微镜下，在睾丸中观察到精子发生的几个阶段，发生在球形卵泡中，与其他黄貂鱼相似。附睾是由一个小管细分成各种小管而形成的。不同的管道与附睾相连，管腔内充满精子。间质腺由腺单位组成，在一些管腔内含有嗜酸性粒细胞，不同的导管平行于腹侧部分。精囊有许多储存精子的小室，有一层类似于蜂巢的壁，管腔里充满了精子。间质腺、输精管和精囊中Alcian Blue (AB)和Periodic Schiff-Acid (PAS)仅在结缔组织中呈阳性，纤毛呈PAS+，细胞核呈弱AB染色。钩腺由单位腺组成，外部被横纹肌覆盖。用周期性席夫酸染色效果很好。雄尾黄貂鱼的生殖道形态与其他黄貂鱼相似。
{"title":"Morphological aspects and germ cells of male reproductive tract of river stingray, Potamotrygon amandae","authors":"Stefhani Cristina Chan Jacik, J. Aquino, L. Melo, R. Rodrigues, A. Melo, M. Miglino, R. Rici","doi":"10.4025/actascibiolsci.v45i1.63722","DOIUrl":"https://doi.org/10.4025/actascibiolsci.v45i1.63722","url":null,"abstract":"The family Potamotrygonidae are the only species of stingrays restricted to fresh water and located exclusively in South America. The objective of this research was to analyze the morphological aspects and germ cells of the male reproductive tract of Potamotrygon amandae. The samples were fixed in 10% formalin, and then dehydrated in an ascending ethanol series (70 to 100%). To carry out light microscopy analyses, they were embedded in paraffin, cut and stained; as for scanning electron microscopy analyses, the samples were dried, glued in metallic bases and metalized. The gross morphology consisted of the following paired organs: testis, epididymis, deferent duct, Leydig gland, seminal vesicle, clasper, and the clasper gland. Microscopically, several stages of spermatogenesis were observed in the testis, occurring in spherical follicles, similar to other stingrays. The epididymis was formed by one duct subdivided in various tubules. The deferent ducts were continuous with the epididymis, and the lumen was full of spermatozoa. The Leydig glands consisted of glandular units with eosinophilic content in the lumen of some, and the deferent ducts ran parallel to the ventral portion. The seminal vesicles possessed numerous compartments to store the sperm, with a wall similar to a hive, and the lumen was full of spermatozoa. Alcian Blue (AB) and Periodic Schiff-Acid (PAS) performed in the Leydig Gland, deferens ducts and seminal vesicle was positive only in the connective tissue, the cilia were PAS+ and the nuclei stained weakly for AB. The clasper gland was composed of unit glands and was covered with striated muscle externally. It stained very well with Periodic Schiff-Acid. The morphological aspects of the male reproductive tract of Potamotrygon amandae were similar to other stingrays.","PeriodicalId":7166,"journal":{"name":"Acta Scientiarum. Biological Sciences","volume":"252 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74988240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-19DOI: 10.4025/actascibiolsci.v45i1.63386
C. Lacerda, C. M. Soares, C. Hayashi
The increasing losses of original features of many riverine environments and the consequent decline of native aquatic species are now a widely recognized problem. The main river basins of South America have been undergoing constant changes in their communities of fish, with native migratory species disappearing and the rising of sedentary exotic ones. However, few studies report experiments that qualify and/or quantify this correlation. Three experiments were conducted to evaluate the predation mortality of two species of fish larvae (native and non-native) regarding their habitat features. The hypothesis that natural features of habitat (aquatic vegetation and water turbidity) controls the survival rates of fish larvae was tested. The experiments highlight the importance of community structure on population dynamics. The native fish larvae showed to be more adapted than the non-native to using the aquatic plants for refuge. The habitat complexity can be closely related to the persistence of native migratory fish species.
{"title":"The role of habitat complexity in the survival rates of migratory (native) and sedentary (non-native) species of fish larvae","authors":"C. Lacerda, C. M. Soares, C. Hayashi","doi":"10.4025/actascibiolsci.v45i1.63386","DOIUrl":"https://doi.org/10.4025/actascibiolsci.v45i1.63386","url":null,"abstract":"The increasing losses of original features of many riverine environments and the consequent decline of native aquatic species are now a widely recognized problem. The main river basins of South America have been undergoing constant changes in their communities of fish, with native migratory species disappearing and the rising of sedentary exotic ones. However, few studies report experiments that qualify and/or quantify this correlation. Three experiments were conducted to evaluate the predation mortality of two species of fish larvae (native and non-native) regarding their habitat features. The hypothesis that natural features of habitat (aquatic vegetation and water turbidity) controls the survival rates of fish larvae was tested. The experiments highlight the importance of community structure on population dynamics. The native fish larvae showed to be more adapted than the non-native to using the aquatic plants for refuge. The habitat complexity can be closely related to the persistence of native migratory fish species.","PeriodicalId":7166,"journal":{"name":"Acta Scientiarum. Biological Sciences","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83289543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-19DOI: 10.4025/actascibiolsci.v45i1.63221
Ouahiba Benhamada, E. Laib, Nabila Benhamada, Sarah Charef, Madjeda Chennah, Sara Chennouf, Hanane Derbak, E. Leghouchi
The assessment of the air quality is a major concern to the current time. The monitoring and maintenance of air quality necessarily pass by detecting and estimating the overall air pollution. The use of lichens must be an assessmenttool to be studied. In our work we were interested about the toxicity of lead on the various parameters of stress in the lichen Xanthoria parietina. For this purpose, lichen thalli have been incubated at lead concentrations of 0, 0.5, 1.0, 5.0 and 10.0 mM, for time scale of 0, 24, 48 and 96 hours. The obtained results showed that lead has an action on the various studied parameters, and the intensity of oxidative stress observed in lichens thalli depends on the concentration, and time of exposure. Lead induced a decrease in chlorophyll and protein contents, and an increase in the contents of catalase, hydrogen peroxide and reduced glutathione. Furthermore, the results also showed that high concentrations of lead caused total destruction of reduced glutathione.
{"title":"Oxidative stress caused by lead in the lichen Xanthoria parietina","authors":"Ouahiba Benhamada, E. Laib, Nabila Benhamada, Sarah Charef, Madjeda Chennah, Sara Chennouf, Hanane Derbak, E. Leghouchi","doi":"10.4025/actascibiolsci.v45i1.63221","DOIUrl":"https://doi.org/10.4025/actascibiolsci.v45i1.63221","url":null,"abstract":"The assessment of the air quality is a major concern to the current time. The monitoring and maintenance of air quality necessarily pass by detecting and estimating the overall air pollution. The use of lichens must be an assessmenttool to be studied. In our work we were interested about the toxicity of lead on the various parameters of stress in the lichen Xanthoria parietina. For this purpose, lichen thalli have been incubated at lead concentrations of 0, 0.5, 1.0, 5.0 and 10.0 mM, for time scale of 0, 24, 48 and 96 hours. The obtained results showed that lead has an action on the various studied parameters, and the intensity of oxidative stress observed in lichens thalli depends on the concentration, and time of exposure. Lead induced a decrease in chlorophyll and protein contents, and an increase in the contents of catalase, hydrogen peroxide and reduced glutathione. Furthermore, the results also showed that high concentrations of lead caused total destruction of reduced glutathione.","PeriodicalId":7166,"journal":{"name":"Acta Scientiarum. Biological Sciences","volume":"54 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72642832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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