P. Andrlová, M. Mráčková, E. Jánová, F. Kološ, M. Sedlinská
The aim of this study was to evaluate the effect of different glycerol concentrations and different sperm concentrations on stallion epididymal sperm motility indicators after thawing. For statistical analysis, 25 stallions were used. Collection of the epidydimal spermatozoa was performed as a retrograde flush of cauda epididymis and ductus deferens within 12 h post castration. After evaluation, the resuspended spermatozoa were centrifuged, the supernatant removed and the spermatozoa resuspended in Gent semen extender to get three different groups with different concentrations of sperm (250 × 106 in ml, 500 × 106 in ml, 1000 × 106 in ml) and different final glycerol concentrations (2%, 4%, and 6%). Therefore, 9 different samples were finally obtained from each stallion. The spermatozoa were packed and placed in a fridge (4 °C) for 2 h, then placed in liquid nitrogen vapour (−80 to −100 °C) and after 25 min plunged into the liquid nitrogen and stored at −196 °C for at least 5 days. The selected straws were individually thawed in a 38 °C water bath for 30 s prior to post-freezing analysis. Motility indicators were assessed at 0, 60, 120, and 180 min after thawing. Parametric test was used for analysis; the measured indicators were total motility, progressive motility, curvilinear velocity, straightness, and average-path velocity. In this study, the best results were reached in samples diluted into a concentration of 1,000 × 106 in ml, regardless of the concentrations of glycerol.
{"title":"Effect of sperm and glycerol concentration on epididymal sperm motility parameters after thawing","authors":"P. Andrlová, M. Mráčková, E. Jánová, F. Kološ, M. Sedlinská","doi":"10.2754/avb202392010021","DOIUrl":"https://doi.org/10.2754/avb202392010021","url":null,"abstract":"The aim of this study was to evaluate the effect of different glycerol concentrations and different sperm concentrations on stallion epididymal sperm motility indicators after thawing. For statistical analysis, 25 stallions were used. Collection of the epidydimal spermatozoa was performed as a retrograde flush of cauda epididymis and ductus deferens within 12 h post castration. After evaluation, the resuspended spermatozoa were centrifuged, the supernatant removed and the spermatozoa resuspended in Gent semen extender to get three different groups with different concentrations of sperm (250 × 106 in ml, 500 × 106 in ml, 1000 × 106 in ml) and different final glycerol concentrations (2%, 4%, and 6%). Therefore, 9 different samples were finally obtained from each stallion. The spermatozoa were packed and placed in a fridge (4 °C) for 2 h, then placed in liquid nitrogen vapour (−80 to −100 °C) and after 25 min plunged into the liquid nitrogen and stored at −196 °C for at least 5 days. The selected straws were individually thawed in a 38 °C water bath for 30 s prior to post-freezing analysis. Motility indicators were assessed at 0, 60, 120, and 180 min after thawing. Parametric test was used for analysis; the measured indicators were total motility, progressive motility, curvilinear velocity, straightness, and average-path velocity. In this study, the best results were reached in samples diluted into a concentration of 1,000 × 106 in ml, regardless of the concentrations of glycerol.","PeriodicalId":7192,"journal":{"name":"Acta Veterinaria Brno","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69209625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I. Hájek, P. Schánilec, Ivana Váňová, M. Pyszko, J. Dufek
Electroneurography measures the speed of the action potential after nerve stimulation. The aim of this study was to evaluate the motor nerve conduction velocity in the tibial nerve after surface stimulation in healthy dogs, to correlate the obtained data by biological factors, and to compare these results with published findings utilizing needle electrodes. The study was performed in 11 clinically healthy dogs without anaesthesia. There were significant correlations among several indicators (age, limb length, conduction velocity, latency and duration of compound muscle action potentials). Age was found to have a significant effect on the duration of the compound muscle action potential from both stimulation sites (proximal/distal; r = 0.68, r = 0.71, P < 0.05), but there was no effect on the conduction velocity or amplitude of the compound muscle action potentials. Limb length was found to have a significant effect on the duration of the compound muscle action potential from the distal stimulation site (r = 0.64, P < 0.05), a significant effect on the conduction velocity (r = −0.91, P < 0.01), and a significant effect on the amplitude of the compound muscle action potential from both stimulation sites (proximal/distal; r = −0.76, r = −0.63, P < 0.01, P < 0.05 respectively). These results indicate that limb length should be considered as the most important biological factor in tibial nerve conduction studies and that electroneurography in dogs could be performed non-invasively, without a danger of infection, haematomas or complications related to anaesthesia.
神经电图测量神经刺激后动作电位的速度。本研究的目的是评估健康犬在表面刺激后胫神经的运动神经传导速度，通过生物学因素将获得的数据联系起来，并将这些结果与已发表的针电极研究结果进行比较。这项研究在11只临床健康的狗身上进行，没有麻醉。年龄、肢体长度、传导速度、肌肉复合动作电位潜伏期、持续时间等指标之间存在显著相关性。研究发现，年龄对两个刺激点(近端/远端;r = 0.68, r = 0.71, P < 0.05)，但对复合肌动作电位的传导速度和振幅无影响。肢体长度对远端刺激部位复合肌动作电位持续时间有显著影响(r = 0.64, P < 0.05)，对传导速度有显著影响(r = - 0.91, P < 0.01)，对两个刺激部位复合肌动作电位振幅均有显著影响(近端/远端;r = - 0.76, r = - 0.63, P < 0.01, P < 0.05)。这些结果表明，在胫骨神经传导研究中，肢体长度应被视为最重要的生物学因素，狗的神经电图可以无创地进行，没有感染、血肿或麻醉相关并发症的危险。
{"title":"Surface electroneurography of the tibial nerve in dogs","authors":"I. Hájek, P. Schánilec, Ivana Váňová, M. Pyszko, J. Dufek","doi":"10.2754/avb202392020151","DOIUrl":"https://doi.org/10.2754/avb202392020151","url":null,"abstract":"Electroneurography measures the speed of the action potential after nerve stimulation. The aim of this study was to evaluate the motor nerve conduction velocity in the tibial nerve after surface stimulation in healthy dogs, to correlate the obtained data by biological factors, and to compare these results with published findings utilizing needle electrodes. The study was performed in 11 clinically healthy dogs without anaesthesia. There were significant correlations among several indicators (age, limb length, conduction velocity, latency and duration of compound muscle action potentials). Age was found to have a significant effect on the duration of the compound muscle action potential from both stimulation sites (proximal/distal; r = 0.68, r = 0.71, P < 0.05), but there was no effect on the conduction velocity or amplitude of the compound muscle action potentials. Limb length was found to have a significant effect on the duration of the compound muscle action potential from the distal stimulation site (r = 0.64, P < 0.05), a significant effect on the conduction velocity (r = −0.91, P < 0.01), and a significant effect on the amplitude of the compound muscle action potential from both stimulation sites (proximal/distal; r = −0.76, r = −0.63, P < 0.01, P < 0.05 respectively). These results indicate that limb length should be considered as the most important biological factor in tibial nerve conduction studies and that electroneurography in dogs could be performed non-invasively, without a danger of infection, haematomas or complications related to anaesthesia.","PeriodicalId":7192,"journal":{"name":"Acta Veterinaria Brno","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69209861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aikaterini M. Zisopoulou, Natália Vyhnalová, E. Jánová, F. Kološ, Š. Krisová
The impact of central corneal thickness (CCT) on intraocular pressure (IOP) has been demonstrated in humans and various animal species but not yet in horses. The current study investigated the relationship between IOP and CCT in horses of different ages, sex, breed, and body weights using tonometry and pachymetry. Ninety-seven horses without ocular disease were examined at the Equine Clinic of the University of Veterinary Sciences Brno, Czech Republic, between 2019 and 2020. A complete ophthalmological examination was performed, including direct ophthalmoscopy and slit-lamp biomicroscopy, to include only horses with healthy eyes in the study. The central corneal thickness was measured with a pachymeter, and the IOP was measured with a tonometer. The effects of sex, age, breed, and horse weight on IOP and CCT were analysed. Measurements of IOP and CCT were acquired in all 97 horses. There was a significant correlation between CCT and age and weight in both eyes. Older and heavier individuals had high CCT values. Mares had lower IOP mean values compared to geldings and stallions. No correlation was found between IOP and CCT of the same eye. This study confirmed a relationship between individual variables such as age and weight on CCT, and sex on IOP. Based on this report, CCT should be considered a minor indicator when interpreting IOP values in healthy horses. Nevertheless, age and weight are essential constraints when interpreting CCT values and sex on IOP values in horses.
{"title":"The correlation between the intraocular pressure, central corneal thickness, and signalment of the horse","authors":"Aikaterini M. Zisopoulou, Natália Vyhnalová, E. Jánová, F. Kološ, Š. Krisová","doi":"10.2754/avb202392030271","DOIUrl":"https://doi.org/10.2754/avb202392030271","url":null,"abstract":"The impact of central corneal thickness (CCT) on intraocular pressure (IOP) has been demonstrated in humans and various animal species but not yet in horses. The current study investigated the relationship between IOP and CCT in horses of different ages, sex, breed, and body weights using tonometry and pachymetry. Ninety-seven horses without ocular disease were examined at the Equine Clinic of the University of Veterinary Sciences Brno, Czech Republic, between 2019 and 2020. A complete ophthalmological examination was performed, including direct ophthalmoscopy and slit-lamp biomicroscopy, to include only horses with healthy eyes in the study. The central corneal thickness was measured with a pachymeter, and the IOP was measured with a tonometer. The effects of sex, age, breed, and horse weight on IOP and CCT were analysed. Measurements of IOP and CCT were acquired in all 97 horses. There was a significant correlation between CCT and age and weight in both eyes. Older and heavier individuals had high CCT values. Mares had lower IOP mean values compared to geldings and stallions. No correlation was found between IOP and CCT of the same eye. This study confirmed a relationship between individual variables such as age and weight on CCT, and sex on IOP. Based on this report, CCT should be considered a minor indicator when interpreting IOP values in healthy horses. Nevertheless, age and weight are essential constraints when interpreting CCT values and sex on IOP values in horses.","PeriodicalId":7192,"journal":{"name":"Acta Veterinaria Brno","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69210035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The aim of this study was to evaluate data on doping controls in racehorses over a given ten-year period, primarily to identify positive findings and to summarise recommendations for the prevention of accidental contamination with prohibited substances, where appropriate. Data on doping controls of racehorses in the Czech Republic from 2010 to 2019 were obtained from the archives of the Jockey Club of the Czech Republic. For each year, the total number of horses starting at races held in the Czech Republic, the number of horses tested, and the results of the doping controls were determined. Data on the type of samples, positive findings and statements from responsible persons about the cause of the positive finding were recorded. During the monitoring period, 11,852 horses competed in races in the Czech Republic and 641 of them underwent a doping control. Blood was taken from 356 horses as the sample for testing and urine was collected from 285 horses. A total of 13 positive findings (2.03% of the 641 tested) were found during the period, namely of morphine, caffeine, theobromine, omeprazole sulphide, furosemide, clenbuterol, norketamine, ritalinic acid, dexamethasone, flunixin, hydroxylidocaine and oripavine. The most common cause, in a total of seven horses, was confirmed as suspected feed contamination. Prevention of positive doping results in our circumstances should therefore be directed primarily towards compliance with proper feed and stable management.
{"title":"Doping control in horses in the Czech Republic in 2010-2019","authors":"Taťána Hytychová, Klára Dosedělová, P. Jahn","doi":"10.2754/avb202392010039","DOIUrl":"https://doi.org/10.2754/avb202392010039","url":null,"abstract":"The aim of this study was to evaluate data on doping controls in racehorses over a given ten-year period, primarily to identify positive findings and to summarise recommendations for the prevention of accidental contamination with prohibited substances, where appropriate. Data on doping controls of racehorses in the Czech Republic from 2010 to 2019 were obtained from the archives of the Jockey Club of the Czech Republic. For each year, the total number of horses starting at races held in the Czech Republic, the number of horses tested, and the results of the doping controls were determined. Data on the type of samples, positive findings and statements from responsible persons about the cause of the positive finding were recorded. During the monitoring period, 11,852 horses competed in races in the Czech Republic and 641 of them underwent a doping control. Blood was taken from 356 horses as the sample for testing and urine was collected from 285 horses. A total of 13 positive findings (2.03% of the 641 tested) were found during the period, namely of morphine, caffeine, theobromine, omeprazole sulphide, furosemide, clenbuterol, norketamine, ritalinic acid, dexamethasone, flunixin, hydroxylidocaine and oripavine. The most common cause, in a total of seven horses, was confirmed as suspected feed contamination. Prevention of positive doping results in our circumstances should therefore be directed primarily towards compliance with proper feed and stable management.","PeriodicalId":7192,"journal":{"name":"Acta Veterinaria Brno","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69209487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. Illek, S. Dresler, M. Šoch, N. Kernerová, Kristýna Šimák Líbalová, Eliška Zevlová, Nikola Havrdová
The objective of the study was to evaluate the effect of organically chelated zinc methionine (Zn-Met) supplementation on lactation performance of dairy cattle, i.e. milk yield (MY), milk components, and the somatic cell count (SCC) in early lactation. Eighty multiparous cows received two treatments (n = 40 per group) from 21 days prepartum throughout 14 weeks post partum (p.p.). Treatments consisted of 1) supplemental Zn-Met at the dose of 30 mg Zn/kg dry matter (DM) of the total mixed ration (TMR) for the supplemented (S) group; and 2) basal TMR without supplementation for the control (C) group. Daily MY of Zn supplemented cows 30 days p.p. was in not significantly affected (P > 0.05) by the treatment. After 60 days, the MY of the S-group was significantly higher (S: 44.70 ± 4.24 kg vs. C: 42.08 ± 4.60 kg, respectively); (P < 0.05). Ninety days p.p. there was a highly significant difference (P < 0.005) as well (S: 43.22 ± 4.47 kg vs. C: 39.98 ± 4.80 kg). Supplemented cows also produced significantly more milk fat and milk protein than the control on days 60 and 90 of the trial. There was a significant drop and a decreasing trend during the entire trial in SCC in S-group. Milk quality and udder health were positively affected by the Zn-Met treatment. This finding has an important implication for veterinary practice in an era of antibiotic reduction.
本研究旨在评价饲粮中添加有机螯合蛋氨酸锌(Zn-Met)对泌乳早期奶牛泌乳性能的影响，包括产奶量(MY)、乳成分和体细胞计数(SCC)。80头产牛在产前21天至产后14周期间接受2次治疗，每组40头。处理包括:1)添加(S)组以总混合日粮(TMR)的30 mg Zn/kg干物质(DM)的剂量添加Zn- met;2)对照组(C)不补充基础TMR。补锌对奶牛30天p.p.日代谢能无显著影响(P < 0.05)。60 d后，S组的MY显著高于C组(S: 44.70±4.24 kg vs. C: 42.08±4.60 kg);(p < 0.05)。P < 0.005) (S: 43.22±4.47 kg vs. C: 39.98±4.80 kg)。在试验的第60天和第90天，奶牛的乳脂和乳蛋白产量也显著高于对照组。s组SCC在整个试验过程中有显著下降和下降趋势。锌- met处理对牛奶品质和乳房健康有积极影响。这一发现对抗生素减少时代的兽医实践具有重要意义。
{"title":"Influence of organic zinc on lactation performance and somatic cell count in dairy cows","authors":"J. Illek, S. Dresler, M. Šoch, N. Kernerová, Kristýna Šimák Líbalová, Eliška Zevlová, Nikola Havrdová","doi":"10.2754/avb202392030233","DOIUrl":"https://doi.org/10.2754/avb202392030233","url":null,"abstract":"The objective of the study was to evaluate the effect of organically chelated zinc methionine (Zn-Met) supplementation on lactation performance of dairy cattle, i.e. milk yield (MY), milk components, and the somatic cell count (SCC) in early lactation. Eighty multiparous cows received two treatments (n = 40 per group) from 21 days prepartum throughout 14 weeks post partum (p.p.). Treatments consisted of 1) supplemental Zn-Met at the dose of 30 mg Zn/kg dry matter (DM) of the total mixed ration (TMR) for the supplemented (S) group; and 2) basal TMR without supplementation for the control (C) group. Daily MY of Zn supplemented cows 30 days p.p. was in not significantly affected (P > 0.05) by the treatment. After 60 days, the MY of the S-group was significantly higher (S: 44.70 ± 4.24 kg vs. C: 42.08 ± 4.60 kg, respectively); (P < 0.05). Ninety days p.p. there was a highly significant difference (P < 0.005) as well (S: 43.22 ± 4.47 kg vs. C: 39.98 ± 4.80 kg). Supplemented cows also produced significantly more milk fat and milk protein than the control on days 60 and 90 of the trial. There was a significant drop and a decreasing trend during the entire trial in SCC in S-group. Milk quality and udder health were positively affected by the Zn-Met treatment. This finding has an important implication for veterinary practice in an era of antibiotic reduction.","PeriodicalId":7192,"journal":{"name":"Acta Veterinaria Brno","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69209971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Uçmak, Özge Turna, Zeynep Günay Uçmak, Asuman Erzengin
The aim of this study was to investigate the foetal sex related difference and progression in maternal serum anti-Müllerian hormone (AMH) and equine chorionic gonadotrophin (eCG) concentrations during different points of time in pregnant Arabian mares. The study groups formed by 12 healthy male offspring- and 12 healthy female offspring-foaling mares, designated as group MFM and group FFM, respectively. Peripheral blood samples were collected on the day of natural mating and then monthly until the 6th month (mo) of gestation. Serum AMH was measured in all serum samples; eCG was measured in samples collected from 2 to 5 months Of gestation. Serum AMH concentrations of group FFM at mo 4 and mo 5 (3.89 ± 0.49 ng/ml; 2.89 ± 0.32 ng/ml), were significantly higher than in group MFM (2.11 ± 0.46 ng/ml; 1.87 ± 0.32 ng/ml), (P < 0.05). The mo of gestation (mo 1–6) had no effect on serum AMH concentrations of either group MMF or FFM (P > 0.05). Serum eCG concentrations of group FFM at mo 2 (359.73 ± 41.51 mIU/ml), were significantly higher than in group MFM (255 ± 21.18 mIU/ml) (P < 0.05). Group-time interaction for eCG concentrations at mo 2–4 was non-significant (P > 0.05). Concentrations of serum AMH showed no relationship with corresponding eCG levels at mo 2–4 (P > 0.05). Individual variations in AMH and eCG concentrations and the inability to determine a cut-off point for determination of foetal sex make these hormones unlikely candidates for determining foetal sex in the mare.
{"title":"Evaluation of serum anti-Müllerian hormone (AMH) and equine chorionic gonadotrophin (eCG) concentrations in pregnant mares in relation to foetal sex","authors":"M. Uçmak, Özge Turna, Zeynep Günay Uçmak, Asuman Erzengin","doi":"10.2754/avb202392010031","DOIUrl":"https://doi.org/10.2754/avb202392010031","url":null,"abstract":"The aim of this study was to investigate the foetal sex related difference and progression in maternal serum anti-Müllerian hormone (AMH) and equine chorionic gonadotrophin (eCG) concentrations during different points of time in pregnant Arabian mares. The study groups formed by 12 healthy male offspring- and 12 healthy female offspring-foaling mares, designated as group MFM and group FFM, respectively. Peripheral blood samples were collected on the day of natural mating and then monthly until the 6th month (mo) of gestation. Serum AMH was measured in all serum samples; eCG was measured in samples collected from 2 to 5 months Of gestation. Serum AMH concentrations of group FFM at mo 4 and mo 5 (3.89 ± 0.49 ng/ml; 2.89 ± 0.32 ng/ml), were significantly higher than in group MFM (2.11 ± 0.46 ng/ml; 1.87 ± 0.32 ng/ml), (P < 0.05). The mo of gestation (mo 1–6) had no effect on serum AMH concentrations of either group MMF or FFM (P > 0.05). Serum eCG concentrations of group FFM at mo 2 (359.73 ± 41.51 mIU/ml), were significantly higher than in group MFM (255 ± 21.18 mIU/ml) (P < 0.05). Group-time interaction for eCG concentrations at mo 2–4 was non-significant (P > 0.05). Concentrations of serum AMH showed no relationship with corresponding eCG levels at mo 2–4 (P > 0.05). Individual variations in AMH and eCG concentrations and the inability to determine a cut-off point for determination of foetal sex make these hormones unlikely candidates for determining foetal sex in the mare.","PeriodicalId":7192,"journal":{"name":"Acta Veterinaria Brno","volume":"169 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69209273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P. Konecná, Dominika Macháčová, F. Tichý, L. Fiala, M. Kyllar, J. Lenz
There has been a lack of research regarding endometrial stem cells in farm animals, and particularly, regarding epithelial stem/progenitor cells. We aimed to identify potential stem cell subpopulations in porcine and bovine endometrium by measuring the expression of selected stem cell markers (OCT3/4, CD44, SSEA-1, SOX10, CD73, and CD117) during the follicular and luteal phases of the oestrous cycle. We studied 28 endometrial tissue samples collected from 14 healthy, cycling pigs and cows. The endometrial mucosa of each sample was divided into basal, middle, and luminal portions, and the immunohistochemical staining intensity and percentages of cells that were marker-positive were recorded for each portion of the endometrium. Expression of OCT-3/4 was identified in the glands and stroma, and that of SOX10 and CD117 was identified in stroma of both porcine and bovine endometrium. In porcine endometrium, CD44 was only expressed in the glands, whereas SSEA-1 was expressed in the glands and stroma. In bovine endometrium, CD73 was only expressed in the glands. Differences in the expression of most of these markers were found between phases of the oestrous cycle and/or portions of the endometrial mucosa. Our data indicate the existence of both epithelial and mesenchymal stem cell subpopulations in the endometrium of pigs and cows during their oestrous cycles. The expression pattern of the stem cell marker SSEA-1 in porcine endometrium suggests a possible alternative location of the epithelial stem/progenitor cell population in the middle portion of the endometrial glands.
{"title":"Immunohistochemical analysis of selected stem cell markers including stage-specific mouse embryonic antigen 1 (SSEA-1) in porcine and bovine endometrium throughout the oestrous cycle","authors":"P. Konecná, Dominika Macháčová, F. Tichý, L. Fiala, M. Kyllar, J. Lenz","doi":"10.2754/avb202392030251","DOIUrl":"https://doi.org/10.2754/avb202392030251","url":null,"abstract":"There has been a lack of research regarding endometrial stem cells in farm animals, and particularly, regarding epithelial stem/progenitor cells. We aimed to identify potential stem cell subpopulations in porcine and bovine endometrium by measuring the expression of selected stem cell markers (OCT3/4, CD44, SSEA-1, SOX10, CD73, and CD117) during the follicular and luteal phases of the oestrous cycle. We studied 28 endometrial tissue samples collected from 14 healthy, cycling pigs and cows. The endometrial mucosa of each sample was divided into basal, middle, and luminal portions, and the immunohistochemical staining intensity and percentages of cells that were marker-positive were recorded for each portion of the endometrium. Expression of OCT-3/4 was identified in the glands and stroma, and that of SOX10 and CD117 was identified in stroma of both porcine and bovine endometrium. In porcine endometrium, CD44 was only expressed in the glands, whereas SSEA-1 was expressed in the glands and stroma. In bovine endometrium, CD73 was only expressed in the glands. Differences in the expression of most of these markers were found between phases of the oestrous cycle and/or portions of the endometrial mucosa. Our data indicate the existence of both epithelial and mesenchymal stem cell subpopulations in the endometrium of pigs and cows during their oestrous cycles. The expression pattern of the stem cell marker SSEA-1 in porcine endometrium suggests a possible alternative location of the epithelial stem/progenitor cell population in the middle portion of the endometrial glands.","PeriodicalId":7192,"journal":{"name":"Acta Veterinaria Brno","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69209978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The aim of the study was to evaluate selected properties of giblets (liver, heart, and gizzard) from conventional (C), organic (O), and wild (W) ducks. A total of 24 giblets (24 of each organ) were analysed in each bird group. Production properties, colour indicators, haem pigment content and chemical composition were compared. The heaviest organ with the highest yield was the liver in C and W ducks and the gizzard in O ducks (P < 0.05). The liver and gizzard of W and O ducks were darker (P < 0.05) compared to the same organs in C ducks. The redness of the heart of O ducks was higher (P < 0.05) than that of the heart of both other groups of ducks (C and W). All W duck organs contained (P < 0.05) more haem pigment. The livers of reared ducks (C and O) contained more protein (P < 0.05) than the livers of W birds. The liver of C reared poultry was about 3–4 × fattier (P < 0.05) compared to the liver of the remaining two groups of ducks. The heart of wild ducks had the highest protein and lowest fat content, the heart of ducks from the C breeding system had the least protein, whereas ducks bred organically had the highest amount of fat in the heart. Ducks from the O system had a fattier gizzard compared to other birds (P < 0.05). The highest protein percentages were found in the gizzards of W ducks, the smallest in the gizzards of C poultry.
{"title":"Quantitative and qualitative properties of giblets from conventional, organic, and wild ducks","authors":"F. Abdullah, H. Buchtová","doi":"10.2754/avb202291010107","DOIUrl":"https://doi.org/10.2754/avb202291010107","url":null,"abstract":"The aim of the study was to evaluate selected properties of giblets (liver, heart, and gizzard) from conventional (C), organic (O), and wild (W) ducks. A total of 24 giblets (24 of each organ) were analysed in each bird group. Production properties, colour indicators, haem pigment content and chemical composition were compared. The heaviest organ with the highest yield was the liver in C and W ducks and the gizzard in O ducks (P < 0.05). The liver and gizzard of W and O ducks were darker (P < 0.05) compared to the same organs in C ducks. The redness of the heart of O ducks was higher (P < 0.05) than that of the heart of both other groups of ducks (C and W). All W duck organs contained (P < 0.05) more haem pigment. The livers of reared ducks (C and O) contained more protein (P < 0.05) than the livers of W birds. The liver of C reared poultry was about 3–4 × fattier (P < 0.05) compared to the liver of the remaining two groups of ducks. The heart of wild ducks had the highest protein and lowest fat content, the heart of ducks from the C breeding system had the least protein, whereas ducks bred organically had the highest amount of fat in the heart. Ducks from the O system had a fattier gizzard compared to other birds (P < 0.05). The highest protein percentages were found in the gizzards of W ducks, the smallest in the gizzards of C poultry.","PeriodicalId":7192,"journal":{"name":"Acta Veterinaria Brno","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69208250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Benicie Kosková, C. Ramírez, Z. Filipejová, Meriç Kocatürk, M. Crha
It is a known fact that thyroid hormones have an influence on the heart function. Opinions on hypothyroidism and its effect on dilated cardiomyopathy (DCM) development are controversial and often contradictory. This prospective study examined the potential association between hypothyroidism and DCM in dogs. A total of 35 dogs with DCM were included in the study. Preclinical DCM was diagnosed in 18 patients, and clinical DCM was diagnosed in 17 patients, four of which were also diagnosed with hypothyroidism. There was a positive correlation between the thyroid-stimulating hormone and body weight, left atrium size and the serum NT-proBNP concentration in patients with preclinical DCM. Euthyroid dogs with DCM had higher total thyroxine values and more ventricular premature complexes than hypothyroid dogs. Although our study did not confirm a direct relationship between hypothyroidism and DCM as a possible cause, other correlations were detected that had not yet been described in veterinary medicine.
{"title":"Association between dilated cardiomyopathy and hypothyroidism in dogs","authors":"Benicie Kosková, C. Ramírez, Z. Filipejová, Meriç Kocatürk, M. Crha","doi":"10.2754/avb202291020201","DOIUrl":"https://doi.org/10.2754/avb202291020201","url":null,"abstract":"It is a known fact that thyroid hormones have an influence on the heart function. Opinions on hypothyroidism and its effect on dilated cardiomyopathy (DCM) development are controversial and often contradictory. This prospective study examined the potential association between hypothyroidism and DCM in dogs. A total of 35 dogs with DCM were included in the study. Preclinical DCM was diagnosed in 18 patients, and clinical DCM was diagnosed in 17 patients, four of which were also diagnosed with hypothyroidism. There was a positive correlation between the thyroid-stimulating hormone and body weight, left atrium size and the serum NT-proBNP concentration in patients with preclinical DCM. Euthyroid dogs with DCM had higher total thyroxine values and more ventricular premature complexes than hypothyroid dogs. Although our study did not confirm a direct relationship between hypothyroidism and DCM as a possible cause, other correlations were detected that had not yet been described in veterinary medicine.","PeriodicalId":7192,"journal":{"name":"Acta Veterinaria Brno","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69208359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Krásna, Jakub Hrdy, J. Prodělalová, P. Vasickova
African swine fever (ASF) is highly contagious haemorrhagic viral disease of domestic pigs and wild boars. The causative agent can be transmitted by direct contact with infected animals or via a contaminated environment, fomites, feed, meat and products thereof. Soft ticks (genus Ornithodoros) are known reservoirs and transmission vectors of the virus. As the disease causes serious problems in many countries, rapid detection of the agent and early diagnosis could help in prevention of its spread. Therefore, a multiple-analyte profile (xMAP) technology based on multiple oligonucleotide ligation followed by polymerase chain reaction (MOL-PCR) was introduced and verified. A system targeting two independent loci of the virus genome was designed to increase the likelihood of different strains detection and an internal control was employed to verify the correct course of the analysis. The sensitivity was experimentally determined as 10 genomic copies of the virus in one µl of isolated DNA. The system was verified on samples originating from a recent ASF outbreak in the Czech Republic (six spleen) and the Czech market (eight liver and heart tissues) with real-time polymerase chain reaction used as a reference method. The results of both methods were in agreement, even in samples with a low concentration of the virus genome (9.45 × 101 genomic copies/µl of DNA). The system introduced represents an open method allowing the detection and semi-quantification of up to 50 targets/agents in one reaction. It can, therefore, be used for rapid one-step screening and as an effective tool for risk management.
{"title":"MOL-PCR and xMAP technology – a novel approach to the detection of African swine fever virus DNA","authors":"M. Krásna, Jakub Hrdy, J. Prodělalová, P. Vasickova","doi":"10.2754/avb202291020141","DOIUrl":"https://doi.org/10.2754/avb202291020141","url":null,"abstract":"African swine fever (ASF) is highly contagious haemorrhagic viral disease of domestic pigs and wild boars. The causative agent can be transmitted by direct contact with infected animals or via a contaminated environment, fomites, feed, meat and products thereof. Soft ticks (genus Ornithodoros) are known reservoirs and transmission vectors of the virus. As the disease causes serious problems in many countries, rapid detection of the agent and early diagnosis could help in prevention of its spread. Therefore, a multiple-analyte profile (xMAP) technology based on multiple oligonucleotide ligation followed by polymerase chain reaction (MOL-PCR) was introduced and verified. A system targeting two independent loci of the virus genome was designed to increase the likelihood of different strains detection and an internal control was employed to verify the correct course of the analysis. The sensitivity was experimentally determined as 10 genomic copies of the virus in one µl of isolated DNA. The system was verified on samples originating from a recent ASF outbreak in the Czech Republic (six spleen) and the Czech market (eight liver and heart tissues) with real-time polymerase chain reaction used as a reference method. The results of both methods were in agreement, even in samples with a low concentration of the virus genome (9.45 × 101 genomic copies/µl of DNA). The system introduced represents an open method allowing the detection and semi-quantification of up to 50 targets/agents in one reaction. It can, therefore, be used for rapid one-step screening and as an effective tool for risk management.","PeriodicalId":7192,"journal":{"name":"Acta Veterinaria Brno","volume":"20 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69208384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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