Jaroslav Nádeníček, E. Voslářová, Veronika Vojtkovská, Z. Todorović, V. Vecerek
The aim of the study was to assess the impact of social isolation of rats in the post-weaning period using behavioural tests aimed at assessing cognitive function, anxiety, and depressive-like behaviours. The monitoring was performed in male Wistar rats which were housed after weaning either individually (n = 8) or in pairs (n = 8) for 33 days. In the open field, rats kept in isolation reared less often (P < 0.05) than pair-housed rats. In the elevated plus-maze test, pair-housed rats entered the open arm more frequently (P = 0.002) and stayed in the closed arm less often (P = 0.019) compared to rats housed in isolation. In the forced swim test, climbing was seen more frequently (P = 0.016) in pair-housed rats whereas immobility was more common (P = 0.006) in rats housed individually. In the novel object recognition test, the pair-housed rats preferred (P = 0.014) the novel object whereas there was no difference (P = 0.107) in time spent by exploring familiar and novel objects in rats housed in isolation. Furthermore, juvenile rats housed for 33 days in isolation showed higher (P = 0.003) body weight gain during the monitored period than rats housed for the same period in pairs. Our findings are important not only in terms of assessing the impact of rat housing on their mental and physical development but also in terms of the accurate interpretation of the results of other experiments where the rat is used as a model organism.
{"title":"Social housing promotes cognitive function and reduces anxiety and depressive-like behaviours in rats","authors":"Jaroslav Nádeníček, E. Voslářová, Veronika Vojtkovská, Z. Todorović, V. Vecerek","doi":"10.2754/avb202291040391","DOIUrl":"https://doi.org/10.2754/avb202291040391","url":null,"abstract":"The aim of the study was to assess the impact of social isolation of rats in the post-weaning period using behavioural tests aimed at assessing cognitive function, anxiety, and depressive-like behaviours. The monitoring was performed in male Wistar rats which were housed after weaning either individually (n = 8) or in pairs (n = 8) for 33 days. In the open field, rats kept in isolation reared less often (P < 0.05) than pair-housed rats. In the elevated plus-maze test, pair-housed rats entered the open arm more frequently (P = 0.002) and stayed in the closed arm less often (P = 0.019) compared to rats housed in isolation. In the forced swim test, climbing was seen more frequently (P = 0.016) in pair-housed rats whereas immobility was more common (P = 0.006) in rats housed individually. In the novel object recognition test, the pair-housed rats preferred (P = 0.014) the novel object whereas there was no difference (P = 0.107) in time spent by exploring familiar and novel objects in rats housed in isolation. Furthermore, juvenile rats housed for 33 days in isolation showed higher (P = 0.003) body weight gain during the monitored period than rats housed for the same period in pairs. Our findings are important not only in terms of assessing the impact of rat housing on their mental and physical development but also in terms of the accurate interpretation of the results of other experiments where the rat is used as a model organism.","PeriodicalId":7192,"journal":{"name":"Acta Veterinaria Brno","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69209227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Yarnall, E. Jolivet, M. Chevalier, Arnaud Bolon, M. Tiberghien, M. Huňady, Corinne Philippe
Three field studies (Phase 4, immediate efficacy) were conducted with an inactivated, adjuvanted, tri or quadri-valent respiratory vaccine for cattle (bovine respiratory syncytial virus [BRSV], parainfluenza 3 virus [Pi3V], Mannheimia haemolytica ± bovine viral diarrhoea virus [BVDV]), compared to competitor vaccines, in three different production systems: allotted fattening bulls, beef calves on their farm of birth, allotted veal calves. Mortality, morbidity, pre and post-vaccinal serological data (ELISA and virus neutralising titres) were compared between groups. There were no significant differences in mortality and morbidity between the groups. In the fattening bulls study, significantly fewer bovine respiratory disease treatments were administered to animals in the Bovalto group. Virus neutralising titre results were not different between groups, except for BVDV in Study 3 where a BVDV outbreak was observed.
{"title":"In field review of Bovalto® Respi 3 and 4 efficacy in different production systems","authors":"M. Yarnall, E. Jolivet, M. Chevalier, Arnaud Bolon, M. Tiberghien, M. Huňady, Corinne Philippe","doi":"10.2754/avb202291030217","DOIUrl":"https://doi.org/10.2754/avb202291030217","url":null,"abstract":"Three field studies (Phase 4, immediate efficacy) were conducted with an inactivated, adjuvanted, tri or quadri-valent respiratory vaccine for cattle (bovine respiratory syncytial virus [BRSV], parainfluenza 3 virus [Pi3V], Mannheimia haemolytica ± bovine viral diarrhoea virus [BVDV]), compared to competitor vaccines, in three different production systems: allotted fattening bulls, beef calves on their farm of birth, allotted veal calves. Mortality, morbidity, pre and post-vaccinal serological data (ELISA and virus neutralising titres) were compared between groups. There were no significant differences in mortality and morbidity between the groups. In the fattening bulls study, significantly fewer bovine respiratory disease treatments were administered to animals in the Bovalto group. Virus neutralising titre results were not different between groups, except for BVDV in Study 3 where a BVDV outbreak was observed.","PeriodicalId":7192,"journal":{"name":"Acta Veterinaria Brno","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69208409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Nečasová, Lucie Urbanova, Laura Staňková, Alena Pompová, Z. Filipejová, K. Somerlíková, K. Řeháková, A. Nečas
Protein C activity, a novel prognostic marker, was evaluated in patients with gastric dilatation and volvulus in relation to mortality and severity of the disease. Data on the mortality, duration of clinical signs, degree of gastric torsion, gastric necrosis, splenectomy and occurrence of disseminated intravascular coagulopathy were recorded. Blood samples were obtained at selected time periods: T0 = no longer than 30 min before surgery; T1 = first day after surgery; T2 = second day after surgery. In addition to protein C activity assessment, haematological and haemostaseological analysis was also performed. The mean protein C activity was the lowest at T1 and the highest at T2. No significant relationship between protein C activity and mortality, duration of clinical signs, degree of gastric torsion, gastric necrosis, splenectomy and disseminated intravascular coagulopathy was found in patients with gastric dilatation and volvulus. All patients that died during hospitalisation after surgery were patients with decreased protein C activity at T1. Although protein C activity was not found to be a reliable preoperative prognostic marker in patients with gastric dilatation and volvulus, the study results show it might be a promising marker in postoperative monitoring of these patients.
{"title":"Protein C activity in dogs with gastric dilatation and volvulus","authors":"A. Nečasová, Lucie Urbanova, Laura Staňková, Alena Pompová, Z. Filipejová, K. Somerlíková, K. Řeháková, A. Nečas","doi":"10.2754/avb202291010035","DOIUrl":"https://doi.org/10.2754/avb202291010035","url":null,"abstract":"Protein C activity, a novel prognostic marker, was evaluated in patients with gastric dilatation and volvulus in relation to mortality and severity of the disease. Data on the mortality, duration of clinical signs, degree of gastric torsion, gastric necrosis, splenectomy and occurrence of disseminated intravascular coagulopathy were recorded. Blood samples were obtained at selected time periods: T0 = no longer than 30 min before surgery; T1 = first day after surgery; T2 = second day after surgery. In addition to protein C activity assessment, haematological and haemostaseological analysis was also performed. The mean protein C activity was the lowest at T1 and the highest at T2. No significant relationship between protein C activity and mortality, duration of clinical signs, degree of gastric torsion, gastric necrosis, splenectomy and disseminated intravascular coagulopathy was found in patients with gastric dilatation and volvulus. All patients that died during hospitalisation after surgery were patients with decreased protein C activity at T1. Although protein C activity was not found to be a reliable preoperative prognostic marker in patients with gastric dilatation and volvulus, the study results show it might be a promising marker in postoperative monitoring of these patients.","PeriodicalId":7192,"journal":{"name":"Acta Veterinaria Brno","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69208590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
María Rodríguez, J. Morales, G. Díaz-Amor, A. González-Bulnes, H. Karembé, D. Šperling
The aim of the present study was to evaluate the effect of different treatment protocols for the control of iron-deficiency anaemia and coccidiosis, one combination injection versus injection of iron and oral application of toltrazuril, on the general behaviour of piglets. Piglets were divided into three experimental treatment groups: 24 piglets were left untreated; (control group, C); 24 piglets received an oral administration of generic anticoccidial agent (20 mg/kg BW) plus intramuscular administration of iron dextran (200 mg/ml; 1 ml/piglet) in the same handling (oral + parenteral group, O+P) and 24 piglets received an intramuscular application of the combination product (parenteral group, P). As expected, the handling time was higher in the oral + parenteral group than in the parenteral group. Regarding the behaviour of piglets, the time spent suckling after treatment was variable in the control and oral + parenteral groups, while piglets from the parenteral group spent more time suckling, and hence, their time spent resting was decreased. A statistical trend of lower concentration of cortisol was observed in P compared to O+P after processing. Regarding productivity indices, the average daily gain of piglets during lactation and, subsequently, their weaning weight were higher in P compared to O+P, and similar to the control. In conclusion, administering a combination product injection decreases the time of administration compared to individual treatments.
{"title":"Influence of the application methods of iron/anticoccidial products on the behaviour and associated stress factors of suckling piglets","authors":"María Rodríguez, J. Morales, G. Díaz-Amor, A. González-Bulnes, H. Karembé, D. Šperling","doi":"10.2754/avb202291030251","DOIUrl":"https://doi.org/10.2754/avb202291030251","url":null,"abstract":"The aim of the present study was to evaluate the effect of different treatment protocols for the control of iron-deficiency anaemia and coccidiosis, one combination injection versus injection of iron and oral application of toltrazuril, on the general behaviour of piglets. Piglets were divided into three experimental treatment groups: 24 piglets were left untreated; (control group, C); 24 piglets received an oral administration of generic anticoccidial agent (20 mg/kg BW) plus intramuscular administration of iron dextran (200 mg/ml; 1 ml/piglet) in the same handling (oral + parenteral group, O+P) and 24 piglets received an intramuscular application of the combination product (parenteral group, P). As expected, the handling time was higher in the oral + parenteral group than in the parenteral group. Regarding the behaviour of piglets, the time spent suckling after treatment was variable in the control and oral + parenteral groups, while piglets from the parenteral group spent more time suckling, and hence, their time spent resting was decreased. A statistical trend of lower concentration of cortisol was observed in P compared to O+P after processing. Regarding productivity indices, the average daily gain of piglets during lactation and, subsequently, their weaning weight were higher in P compared to O+P, and similar to the control. In conclusion, administering a combination product injection decreases the time of administration compared to individual treatments.","PeriodicalId":7192,"journal":{"name":"Acta Veterinaria Brno","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69208652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mu-Young Kim, Ji-hyun Kim, Kyuchang Kim, Hun-Young Yoon
A 5-month-old mixed-breed dog and a 10-month-old Welsh corgi dog were presented for evaluation with signs of congenital portosystemic shunt. In both dogs, computed tomography angiography revealed a single extrahepatic portosystemic shunt with the absence of the portal vein cranial to the shunt origin. Intraoperative mesenteric portography (IOMP) was performed after temporary complete occlusion of the shunt, revealing the portal branches faintly opacified with contrast medium. A cellophane band was applied around the shunt vessel without intraoperative attenuation. Postoperatively, both the dogs showed significant clinical improvement and the portal veins, not observed on preoperative ultrasonography, were visualized on postoperative ultrasonography. This study suggests the efficacy of IOMP, enabling the appropriate surgical procedure to be determined.
{"title":"The effectiveness of intraoperative mesenteric portography for preventing misdiagnosis of congenital absence of the portal vein in dog with extrahepatic portosystemic shunt: a case report","authors":"Mu-Young Kim, Ji-hyun Kim, Kyuchang Kim, Hun-Young Yoon","doi":"10.2754/avb202291030267","DOIUrl":"https://doi.org/10.2754/avb202291030267","url":null,"abstract":"A 5-month-old mixed-breed dog and a 10-month-old Welsh corgi dog were presented for evaluation with signs of congenital portosystemic shunt. In both dogs, computed tomography angiography revealed a single extrahepatic portosystemic shunt with the absence of the portal vein cranial to the shunt origin. Intraoperative mesenteric portography (IOMP) was performed after temporary complete occlusion of the shunt, revealing the portal branches faintly opacified with contrast medium. A cellophane band was applied around the shunt vessel without intraoperative attenuation. Postoperatively, both the dogs showed significant clinical improvement and the portal veins, not observed on preoperative ultrasonography, were visualized on postoperative ultrasonography. This study suggests the efficacy of IOMP, enabling the appropriate surgical procedure to be determined.","PeriodicalId":7192,"journal":{"name":"Acta Veterinaria Brno","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69209197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Z. Siroka, P. Linhart, A. Honzlová, Veronika Vlasáková, J. Rosmus, P. Macharáčková, Z. Svobodová, M. Svoboda
Ochratoxin A (OTA) is a mycotoxin produced by several moulds of Aspergillus and Penicillium genera on many agricultural commodities used for feed production. It is primarily a nephrotoxic substance, but it can also cause immunosuppression in pigs and increase their susceptibility to infections. Porcine mycotoxic nephropathy (PMN) is a chronic poisoning associated with OTA exposure. The aim of this paper was to evaluate the content of OTA in pig kidneys which were examined during the regular monitoring of the safety and quality of food of animal origin in the Czech Republic during the years 2012–2021. Results revealed no differences between the monitored years or age categories and no differences between the year seasons, which could have an influence on the storage conditions and thus feed contamination by moulds. The values found in this study are low and similar to those assessed in several studies performed in other European countries and do not exceed the limits set in their legislation, which means these organs do not pose a risk for consumers and do not indicate PMN in monitored pigs. Czech Republic as well as EU legislation do not regulate OTA levels in the food of animal origin yet.
{"title":"A survey on occurrence of ochratoxin A in the kidneys of slaughtered pigs in the Czech Republic during the years 2012–2021","authors":"Z. Siroka, P. Linhart, A. Honzlová, Veronika Vlasáková, J. Rosmus, P. Macharáčková, Z. Svobodová, M. Svoboda","doi":"10.2754/avb202291040409","DOIUrl":"https://doi.org/10.2754/avb202291040409","url":null,"abstract":"Ochratoxin A (OTA) is a mycotoxin produced by several moulds of Aspergillus and Penicillium genera on many agricultural commodities used for feed production. It is primarily a nephrotoxic substance, but it can also cause immunosuppression in pigs and increase their susceptibility to infections. Porcine mycotoxic nephropathy (PMN) is a chronic poisoning associated with OTA exposure. The aim of this paper was to evaluate the content of OTA in pig kidneys which were examined during the regular monitoring of the safety and quality of food of animal origin in the Czech Republic during the years 2012–2021. Results revealed no differences between the monitored years or age categories and no differences between the year seasons, which could have an influence on the storage conditions and thus feed contamination by moulds. The values found in this study are low and similar to those assessed in several studies performed in other European countries and do not exceed the limits set in their legislation, which means these organs do not pose a risk for consumers and do not indicate PMN in monitored pigs. Czech Republic as well as EU legislation do not regulate OTA levels in the food of animal origin yet.","PeriodicalId":7192,"journal":{"name":"Acta Veterinaria Brno","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69209294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The purpose of this pilot study was to assess the efficacy of long-term masitinib therapy at low doses on echocardiographic, cardiovascular, haematological, and blood biochemical indicators, as well as clinical symptoms in dogs with pulmonary hypertension (PH) caused by advanced chronic degenerative mitral valve disease or heartworm disease. Seven client-owned dogs with severe PH were recruited prospectively and given low-dose masitinib orally, 3 mg/kg body weight (approximately one-fourth of the recommended antineoplastic dosage), q24h, for 123–928 days. Examinations were performed prior to masitinib administration, as well as 1, 2, 3, 6, and 12 months later. At 1–12 months, low-dose masitinib significantly reduced systolic pulmonary arterial pressure (P < 0.05 or 0.01) and dramatically improved clinical symptoms. Low-dose masitinib treatment improved right ventricular function indicators such as right atrium/aorta ratio, maximum tricuspid regurgitation velocity, right ventricular Tei index, and tricuspid annular plane systolic excursion, without worsening left ventricular function indicators. These findings suggest that low-dose masitinib may be effective as an adjunctive therapeutic for chronic heart failure in dogs with PH and may increase the survival of PH dogs.
{"title":"Effectiveness of therapy with low-dosage masitinib on pulmonary hypertension in dogs: a pilot study","authors":"Yumi Sahashi, Y. Hikasa","doi":"10.2754/avb202291040363","DOIUrl":"https://doi.org/10.2754/avb202291040363","url":null,"abstract":"The purpose of this pilot study was to assess the efficacy of long-term masitinib therapy at low doses on echocardiographic, cardiovascular, haematological, and blood biochemical indicators, as well as clinical symptoms in dogs with pulmonary hypertension (PH) caused by advanced chronic degenerative mitral valve disease or heartworm disease. Seven client-owned dogs with severe PH were recruited prospectively and given low-dose masitinib orally, 3 mg/kg body weight (approximately one-fourth of the recommended antineoplastic dosage), q24h, for 123–928 days. Examinations were performed prior to masitinib administration, as well as 1, 2, 3, 6, and 12 months later. At 1–12 months, low-dose masitinib significantly reduced systolic pulmonary arterial pressure (P < 0.05 or 0.01) and dramatically improved clinical symptoms. Low-dose masitinib treatment improved right ventricular function indicators such as right atrium/aorta ratio, maximum tricuspid regurgitation velocity, right ventricular Tei index, and tricuspid annular plane systolic excursion, without worsening left ventricular function indicators. These findings suggest that low-dose masitinib may be effective as an adjunctive therapeutic for chronic heart failure in dogs with PH and may increase the survival of PH dogs.","PeriodicalId":7192,"journal":{"name":"Acta Veterinaria Brno","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69209545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
W. McCartney, C. Ober, M. Benito, B. M. Mac Donald
The ventral slot technique is used to relieve neural compression secondary to intervertebral disc degeneration or disease. In the present study, the biomechanical properties of three different ventral surgical procedures in the rabbit C6–C7 vertebral motion unit (VMU) were assessed and compared with the intact C6–C7 VMU. The ventral slot procedure (slanted, full, or mini; n = 8/group) was performed on these cervical vertebrae. Normal spine torsion and flexion values were compared to those of spines subjected to slanted, full, and mini slot surgery. The slanted slot spines were the most stable, maintaining 70% of normal cervical spine strength, compared to 26% with the full slot and 30% with the mini slot. Regarding torsion, slanted slot spines showed 74% stability compared to the normal cervical spine, while the full slot and mini slot spines showed 58% and 62% stability, respectively. Flexion values were lower after all versions of the ventral slot procedure than in the normal spine, with the greatest flexion difference occurring after the full slot procedure (21% of the normal flexion value). The flexion values also differed significantly between the slanted and full spine groups, and all operated spines showing roughly 60% torsion rates compared with normal spines. The slanted slot maintains more stability in rabbit cervical spine than the other procedures. To our knowledge, this was the first study to examine biomechanical failure differences between the distinct versions of this ventral slot procedure.
{"title":"Slanted slot was the most resistant to failure of ventral slot techniques tested in rabbit cervical vertebrae","authors":"W. McCartney, C. Ober, M. Benito, B. M. Mac Donald","doi":"10.2754/avb202291010043","DOIUrl":"https://doi.org/10.2754/avb202291010043","url":null,"abstract":"The ventral slot technique is used to relieve neural compression secondary to intervertebral disc degeneration or disease. In the present study, the biomechanical properties of three different ventral surgical procedures in the rabbit C6–C7 vertebral motion unit (VMU) were assessed and compared with the intact C6–C7 VMU. The ventral slot procedure (slanted, full, or mini; n = 8/group) was performed on these cervical vertebrae. Normal spine torsion and flexion values were compared to those of spines subjected to slanted, full, and mini slot surgery. The slanted slot spines were the most stable, maintaining 70% of normal cervical spine strength, compared to 26% with the full slot and 30% with the mini slot. Regarding torsion, slanted slot spines showed 74% stability compared to the normal cervical spine, while the full slot and mini slot spines showed 58% and 62% stability, respectively. Flexion values were lower after all versions of the ventral slot procedure than in the normal spine, with the greatest flexion difference occurring after the full slot procedure (21% of the normal flexion value). The flexion values also differed significantly between the slanted and full spine groups, and all operated spines showing roughly 60% torsion rates compared with normal spines. The slanted slot maintains more stability in rabbit cervical spine than the other procedures. To our knowledge, this was the first study to examine biomechanical failure differences between the distinct versions of this ventral slot procedure.","PeriodicalId":7192,"journal":{"name":"Acta Veterinaria Brno","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69208597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Taraxacum officinale (TO) is a well-known medicinal plant used in folk medicine for its variety of biological activities. In this study a methanolic extract from roots was used to examine its anti-tumour effect by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) viability assay on two mouse tumour cell lines, fibrosarcoma and hepatoma cell lines. Normal hepatocyte and fibroblast cell lines were used as a control. Furthermore, three active compounds were isolated from the extract, caffeic acid, chlorogenic acid, and ursolic acid, in order to investigate their cytotoxicity and possible interactions between them in their combinations on the same tumour and non-tumour cell lines. The anti-tumour effect of the TO extract was confirmed on the fibrosarcoma cell line in a dose dependent manner. The anti-proliferative acting of each acid was described on both cancer cell lines and for the first time the combinations of these acids were investigated and their common effect in the mixtures reported. Further experiments to determine the mechanism of action and examine their action with conventional chemotherapeutics as a potential adjuvant therapy to enhance the chemotherapeutic effect and improve patient health with its hepatoprotective activity could be encouraged.
{"title":"Anti-tumour effect of combinations of three acids isolated from Taraxacum officinale","authors":"Markéta Korbášová, J. Tomenendálová, J. Chloupek","doi":"10.2754/avb202291010077","DOIUrl":"https://doi.org/10.2754/avb202291010077","url":null,"abstract":"Taraxacum officinale (TO) is a well-known medicinal plant used in folk medicine for its variety of biological activities. In this study a methanolic extract from roots was used to examine its anti-tumour effect by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) viability assay on two mouse tumour cell lines, fibrosarcoma and hepatoma cell lines. Normal hepatocyte and fibroblast cell lines were used as a control. Furthermore, three active compounds were isolated from the extract, caffeic acid, chlorogenic acid, and ursolic acid, in order to investigate their cytotoxicity and possible interactions between them in their combinations on the same tumour and non-tumour cell lines. The anti-tumour effect of the TO extract was confirmed on the fibrosarcoma cell line in a dose dependent manner. The anti-proliferative acting of each acid was described on both cancer cell lines and for the first time the combinations of these acids were investigated and their common effect in the mixtures reported. Further experiments to determine the mechanism of action and examine their action with conventional chemotherapeutics as a potential adjuvant therapy to enhance the chemotherapeutic effect and improve patient health with its hepatoprotective activity could be encouraged.","PeriodicalId":7192,"journal":{"name":"Acta Veterinaria Brno","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69208665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
B. Çelik, Ö. Çelik, Adnan Ayan, A. Yılmaz, Ö. Kilinç, Ö. Ayan
This study aimed to determine Hepatozoon canis prevalence in dogs in the Siirt province of Turkey by the molecular method. The animal material of the study consisted of a total of 75 dogs that appeared clinically healthy. Two ml of blood sample were taken from the vena cephalica antebrachii. Then, DNA extraction was performed. Polymerase chain reaction (PCR) was performed to amplify the 666 bp 18S rRNA gene region of Hepatozoon canis. Two positive PCR products were purified and sequenced. As a result of Nested-PCR, H. canis specific bands in 666 bp size were obtained in 7 (9.33%) out of 75 dogs. The result of sequence analysis, the nucleotide sequence was registered in the NCBI GenBank database with accession numbers OL467380.1-OL467538.1. Hepatozoon canis registered in GenBank of sequence OL467380.1 was found to be similar with other H. canis strains of registration numbers MW684292.1 with 99.69% and MH615006.1-MK091085.1-MF797806.1 with 99.53% rates; and the sequence with registration number OL467538.1 was found to be similar to the series MW684291.1 with 99.09% and MH615006.1-MK091085.1-KX 818220.1 with 99.08% rates by BLAST analysis. Hepatozoon canis prevalence of dogs in the Siirt province was determined as a result of this study. It is of great importance to take preventive measures, especially to fight ticks with appropriate acaricides, since there is no vaccine to prevent the disease.
{"title":"A Molecular survey of Hepatozoon canis in dogs in the Siirt province of Turkey","authors":"B. Çelik, Ö. Çelik, Adnan Ayan, A. Yılmaz, Ö. Kilinç, Ö. Ayan","doi":"10.2754/avb202291030277","DOIUrl":"https://doi.org/10.2754/avb202291030277","url":null,"abstract":"This study aimed to determine Hepatozoon canis prevalence in dogs in the Siirt province of Turkey by the molecular method. The animal material of the study consisted of a total of 75 dogs that appeared clinically healthy. Two ml of blood sample were taken from the vena cephalica antebrachii. Then, DNA extraction was performed. Polymerase chain reaction (PCR) was performed to amplify the 666 bp 18S rRNA gene region of Hepatozoon canis. Two positive PCR products were purified and sequenced. As a result of Nested-PCR, H. canis specific bands in 666 bp size were obtained in 7 (9.33%) out of 75 dogs. The result of sequence analysis, the nucleotide sequence was registered in the NCBI GenBank database with accession numbers OL467380.1-OL467538.1. Hepatozoon canis registered in GenBank of sequence OL467380.1 was found to be similar with other H. canis strains of registration numbers MW684292.1 with 99.69% and MH615006.1-MK091085.1-MF797806.1 with 99.53% rates; and the sequence with registration number OL467538.1 was found to be similar to the series MW684291.1 with 99.09% and MH615006.1-MK091085.1-KX 818220.1 with 99.08% rates by BLAST analysis. Hepatozoon canis prevalence of dogs in the Siirt province was determined as a result of this study. It is of great importance to take preventive measures, especially to fight ticks with appropriate acaricides, since there is no vaccine to prevent the disease.","PeriodicalId":7192,"journal":{"name":"Acta Veterinaria Brno","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69208808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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