I. Mikulíková, M. Palíková, I. Papežíková, J. Blahova, Ivona Toulová, H. Minářová
Analyses of blood samples in ichthyology are of importance for assessment of fish health as well as fish responses to environmental stressors. The measurement results may be affected by multiple factors. This study aimed at assessment of the influence of the blood collection site by comparing dual values of indices measured in samples obtained both from the heart and puncturing caudal vessels in the same fish specimens. Our results revealed that the sampling site did not significantly influence measured variables including haematological indices, the plasma biochemistry profile, acid-base balance parameters and the phagocytic activity. To conclude, for the rainbow trout (Oncorhynchus mykiss) both sampling methods are interchangeable with regard to the above-mentioned indices.
{"title":"Does blood sampling from caudal vessels in fish produce parameter values different from those obtained by heart puncture?","authors":"I. Mikulíková, M. Palíková, I. Papežíková, J. Blahova, Ivona Toulová, H. Minářová","doi":"10.2754/avb202291010069","DOIUrl":"https://doi.org/10.2754/avb202291010069","url":null,"abstract":"Analyses of blood samples in ichthyology are of importance for assessment of fish health as well as fish responses to environmental stressors. The measurement results may be affected by multiple factors. This study aimed at assessment of the influence of the blood collection site by comparing dual values of indices measured in samples obtained both from the heart and puncturing caudal vessels in the same fish specimens. Our results revealed that the sampling site did not significantly influence measured variables including haematological indices, the plasma biochemistry profile, acid-base balance parameters and the phagocytic activity. To conclude, for the rainbow trout (Oncorhynchus mykiss) both sampling methods are interchangeable with regard to the above-mentioned indices.","PeriodicalId":7192,"journal":{"name":"Acta Veterinaria Brno","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69208615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study focused on finding new information regarding the assessment of pig saliva cortisol samples in terms of practical effects of the sampling, sample storage conditions, and their laboratory analysis. The study was divided into two experiments. The first experiment was focused on finding the effect of sampling time on cortisol concentrations in pig saliva. The second experiment was focused on determining the effect of storage conditions on the value of salivary cortisol. Before the initiation of the study, we tested which one of the commercially available ELISA kits would be the most suitable for our experiments. Simultaneously, we carried out a pre-study to evaluate the effect of relocation and change in the housing type on the concentration of salivary cortisol in gestating sows. The samples were obtained by oral cavity swabbing, using a standard cotton swab. In the first study, piglets were examined at the age of 4 ± 1 days, and breeding management routine procedures were used as a stress factor. In the second study, the piglets were examined immediately after weaning (at 28 ± 2 days of age). The Cortisol EIA kit was found to be statistically more accurate and thus a more suitable ELISA kit for our experiment. Analysis of the relocation effect and the effect of change in the housing type showed that relocation does not seem to be a stress factor for gestating sows as no significant changes were observed in salivary cortisol concentration (P > 0.5); however, the change in the housing type lead to a significant increase in salivary cortisol (P < 0.001). In the first study, we determined using the ELISA method that the most significant difference occurred in 40 min (P < 0.01), which suggests that the best time for a sampling in order to assess salivary cortisol concentration is 40 min after stress induction by routine procedures. The conclusion of the second study was that in the monitored period of 60 h (P < 0.05), cortisol concentration decreased depending on the storage temperature. The decrease started between 48 and 60 h which showed that cortisol is stable in the saliva sample for at least 48 h. These findings will be further applied in our following studies focused on assessment of salivary cortisol concentration after stress induction.
{"title":"Sampling methods for determination of cortisol in pig saliva and their use in the assessment of pig welfare","authors":"Michaela Němečková, Tereza Popelková, P. Chloupek","doi":"10.2754/avb202291030261","DOIUrl":"https://doi.org/10.2754/avb202291030261","url":null,"abstract":"This study focused on finding new information regarding the assessment of pig saliva cortisol samples in terms of practical effects of the sampling, sample storage conditions, and their laboratory analysis. The study was divided into two experiments. The first experiment was focused on finding the effect of sampling time on cortisol concentrations in pig saliva. The second experiment was focused on determining the effect of storage conditions on the value of salivary cortisol. Before the initiation of the study, we tested which one of the commercially available ELISA kits would be the most suitable for our experiments. Simultaneously, we carried out a pre-study to evaluate the effect of relocation and change in the housing type on the concentration of salivary cortisol in gestating sows. The samples were obtained by oral cavity swabbing, using a standard cotton swab. In the first study, piglets were examined at the age of 4 ± 1 days, and breeding management routine procedures were used as a stress factor. In the second study, the piglets were examined immediately after weaning (at 28 ± 2 days of age). The Cortisol EIA kit was found to be statistically more accurate and thus a more suitable ELISA kit for our experiment. Analysis of the relocation effect and the effect of change in the housing type showed that relocation does not seem to be a stress factor for gestating sows as no significant changes were observed in salivary cortisol concentration (P > 0.5); however, the change in the housing type lead to a significant increase in salivary cortisol (P < 0.001). In the first study, we determined using the ELISA method that the most significant difference occurred in 40 min (P < 0.01), which suggests that the best time for a sampling in order to assess salivary cortisol concentration is 40 min after stress induction by routine procedures. The conclusion of the second study was that in the monitored period of 60 h (P < 0.05), cortisol concentration decreased depending on the storage temperature. The decrease started between 48 and 60 h which showed that cortisol is stable in the saliva sample for at least 48 h. These findings will be further applied in our following studies focused on assessment of salivary cortisol concentration after stress induction.","PeriodicalId":7192,"journal":{"name":"Acta Veterinaria Brno","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69209177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lucie Urbanova, Ondrej Mišo, A. Nečasová, Z. Filipejová, K. Řeháková, A. Nečas
This study evaluated selected biochemical indices in blood/peritoneal fluid in dogs with and without gastrointestinal tract damage. Blood and peritoneal fluid samples of 29 dogs presented to the Small Animal Clinic at the University of Veterinary and Pharmaceutical Sciences Brno were obtained as a part of the diagnostic approach and were subsequently biochemically analysed. The biochemical indices analysed were potassium, total protein, glucose, lactate and creatinine. Effusion-to-blood concentration ratios of selected indices were obtained for higher data validity and this ratio was then compared between two groups: patients with gastrointestinal tract damage and patients without gastrointestinal tract damage. There were 11 dogs in the study group of patients with gastrointestinal tract damage and 18 dogs in the study group of patients without gastrointestinal tract damage. Subsequent statistical data analysis did not show any significant difference between these two groups in any of the selected indices. According to our study results, selected biochemical indices cannot be considered as decisive for diagnosing gastrointestinal tract damage.
{"title":"Use of selected biochemical indices in blood/peritoneal effusion in diagnostics of gastrointestinal tract damage in dogs","authors":"Lucie Urbanova, Ondrej Mišo, A. Nečasová, Z. Filipejová, K. Řeháková, A. Nečas","doi":"10.2754/avb202291020157","DOIUrl":"https://doi.org/10.2754/avb202291020157","url":null,"abstract":"This study evaluated selected biochemical indices in blood/peritoneal fluid in dogs with and without gastrointestinal tract damage. Blood and peritoneal fluid samples of 29 dogs presented to the Small Animal Clinic at the University of Veterinary and Pharmaceutical Sciences Brno were obtained as a part of the diagnostic approach and were subsequently biochemically analysed. The biochemical indices analysed were potassium, total protein, glucose, lactate and creatinine. Effusion-to-blood concentration ratios of selected indices were obtained for higher data validity and this ratio was then compared between two groups: patients with gastrointestinal tract damage and patients without gastrointestinal tract damage. There were 11 dogs in the study group of patients with gastrointestinal tract damage and 18 dogs in the study group of patients without gastrointestinal tract damage. Subsequent statistical data analysis did not show any significant difference between these two groups in any of the selected indices. According to our study results, selected biochemical indices cannot be considered as decisive for diagnosing gastrointestinal tract damage.","PeriodicalId":7192,"journal":{"name":"Acta Veterinaria Brno","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69208480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. Voslářová, Dora Olejníková, V. Vecerek, Veronika Vojtkovská, I. Bedáňová
The survival of captive-reared pheasants in the wild depends primarily on their ability to avoid predators, therefore, pheasants need to maintain their innate anti-predatory behaviour. The aim of this study was to assess the effects of age and disturbance on tonic immobility (TI) in pheasants (Phasianus colchicus) kept in a commercial rearing facility. TI tests were performed in pheasants aged 8, 10, 12, 14 and 16 weeks. Randomly selected pheasants of the same age were tested either immediately after capture (group C) or after exposure to various stimuli/disturbance (group D). No significant differences in the number of attempts to induce TI were found between the observed groups of pheasants (the vast majority of pheasants remained immobile on the first attempt). The effect of age was found only in group D, where birds aged 14 and 16 weeks showed a reduction in the duration of TI compared to younger birds. In group C, the duration of TI in pheasants of different ages did not differ. The results document a change in the duration of TI in response to an intense stimulus depending on the age of captive-reared pheasants. Knowledge of changes in anti-predatory behaviour depending on age and habituation to the environment and new stimuli is essential when determining the optimal age for pheasants to be released into the wild considering its impact on the subsequent survival of the released birds. Birds released at an older age and thus accustomed to repeated disturbances during captive rearing may have impaired defence behaviour.
{"title":"Effects of age and disturbance on fear in pheasants kept in a rearing facility","authors":"E. Voslářová, Dora Olejníková, V. Vecerek, Veronika Vojtkovská, I. Bedáňová","doi":"10.2754/avb202291030303","DOIUrl":"https://doi.org/10.2754/avb202291030303","url":null,"abstract":"The survival of captive-reared pheasants in the wild depends primarily on their ability to avoid predators, therefore, pheasants need to maintain their innate anti-predatory behaviour. The aim of this study was to assess the effects of age and disturbance on tonic immobility (TI) in pheasants (Phasianus colchicus) kept in a commercial rearing facility. TI tests were performed in pheasants aged 8, 10, 12, 14 and 16 weeks. Randomly selected pheasants of the same age were tested either immediately after capture (group C) or after exposure to various stimuli/disturbance (group D). No significant differences in the number of attempts to induce TI were found between the observed groups of pheasants (the vast majority of pheasants remained immobile on the first attempt). The effect of age was found only in group D, where birds aged 14 and 16 weeks showed a reduction in the duration of TI compared to younger birds. In group C, the duration of TI in pheasants of different ages did not differ. The results document a change in the duration of TI in response to an intense stimulus depending on the age of captive-reared pheasants. Knowledge of changes in anti-predatory behaviour depending on age and habituation to the environment and new stimuli is essential when determining the optimal age for pheasants to be released into the wild considering its impact on the subsequent survival of the released birds. Birds released at an older age and thus accustomed to repeated disturbances during captive rearing may have impaired defence behaviour.","PeriodicalId":7192,"journal":{"name":"Acta Veterinaria Brno","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69209024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Bora, M. M, D. Mathew, Himasri Das, D. P. Bora, N. Barman
The use of point of care diagnostics (POCD) in animal diseases has steadily increased over the years since its introduction. Its potential application to diagnose infectious diseases in remote and resource limited settings have made it an ideal diagnostic in animal disease diagnosis and surveillance. The rapid increase in incidence of emerging infectious diseases requires urgent attention where POCD could be indispensable tools for immediate detection and early warning of a potential pathogen. The advantages of being rapid, easily affordable and the ability to diagnose an infectious disease on spot has driven an intense effort to refine and build on the existing technologies to generate advanced POCD with incremental improvements in analytical performance to diagnose a broad spectrum of animal diseases. The rural communities in developing countries are invariably affected by the burden of infectious animal diseases due to limited access to diagnostics and animal health personnel. Besides, the alarming trend of emerging and transboundary diseases with pathogen spill-overs at livestock-wildlife interfaces has been identified as a threat to the domestic population and wildlife conservation. Under such circumstances, POCD coupled with non-invasive sampling techniques could be successfully deployed at field level without the use of sophisticated laboratory infrastructures. This review illustrates the current and prospective POCD for existing and emerging animal diseases, the status of non-invasive sampling strategies for animal diseases, and the tremendous potential of POCD to uplift the status of global animal health care.
{"title":"Point of care diagnostics and non-invasive sampling strategy: a review on major advances in veterinary diagnostics","authors":"M. Bora, M. M, D. Mathew, Himasri Das, D. P. Bora, N. Barman","doi":"10.2754/avb202291010017","DOIUrl":"https://doi.org/10.2754/avb202291010017","url":null,"abstract":"The use of point of care diagnostics (POCD) in animal diseases has steadily increased over the years since its introduction. Its potential application to diagnose infectious diseases in remote and resource limited settings have made it an ideal diagnostic in animal disease diagnosis and surveillance. The rapid increase in incidence of emerging infectious diseases requires urgent attention where POCD could be indispensable tools for immediate detection and early warning of a potential pathogen. The advantages of being rapid, easily affordable and the ability to diagnose an infectious disease on spot has driven an intense effort to refine and build on the existing technologies to generate advanced POCD with incremental improvements in analytical performance to diagnose a broad spectrum of animal diseases. The rural communities in developing countries are invariably affected by the burden of infectious animal diseases due to limited access to diagnostics and animal health personnel. Besides, the alarming trend of emerging and transboundary diseases with pathogen spill-overs at livestock-wildlife interfaces has been identified as a threat to the domestic population and wildlife conservation. Under such circumstances, POCD coupled with non-invasive sampling techniques could be successfully deployed at field level without the use of sophisticated laboratory infrastructures. This review illustrates the current and prospective POCD for existing and emerging animal diseases, the status of non-invasive sampling strategies for animal diseases, and the tremendous potential of POCD to uplift the status of global animal health care.","PeriodicalId":7192,"journal":{"name":"Acta Veterinaria Brno","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69208175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Monitoring the activity of radionuclides in food is part of the monitoring system of foreign substances. A close correlation between gamma spectrometry determined by 137Cs activity concentration in the meat and stomach wall (r = 0.984) was found in monitored Slovak wild boars with a low activity of 137Cs . The transfer factors of the soil and the stomach content and the soil and the meat were approximately 0.07 at the median. The median transfer factor between the stomach content and the stomach wall is similar to that of the stomach wall and the meat (2.412 and 1.805). The mean value of 137Cs activity concentration in the muscles of wild boars from the Czech Republic was 46.7 Bq·kg-1. The mean value of 137Cs activity concentration in the stomach wall was 23.7 Bq·kg-1. The mutual correlation was r = 0.988. There was further correlation of 137Cs activity concentration between the heart and the diaphragm (r = 0.977), the heart and the tongue (r = 0.902), and the heart and the lungs (r = 0.906). Based on the results from Slovakia and the Czech Republic, there is hope that it will be possible to use a sample from the stomach wall to determine the activity concentration of 137Cs in meat, thereby replacing the commonly used and highly valued top side sample. In practice, monitoring soil contamination to predict the meat contamination of wild boars is more important than monitoring elements of the food chain. The potassium-caesium coefficient reached 5.57 in the muscles and 17.25 in the stomach wall.
{"title":"Selected aspects of caesium-137 contamination in wild boars (Sus scrofa)","authors":"K. Beňová, A. Havelkova, P. Dvořák","doi":"10.2754/avb202291020149","DOIUrl":"https://doi.org/10.2754/avb202291020149","url":null,"abstract":"Monitoring the activity of radionuclides in food is part of the monitoring system of foreign substances. A close correlation between gamma spectrometry determined by 137Cs activity concentration in the meat and stomach wall (r = 0.984) was found in monitored Slovak wild boars with a low activity of 137Cs . The transfer factors of the soil and the stomach content and the soil and the meat were approximately 0.07 at the median. The median transfer factor between the stomach content and the stomach wall is similar to that of the stomach wall and the meat (2.412 and 1.805). The mean value of 137Cs activity concentration in the muscles of wild boars from the Czech Republic was 46.7 Bq·kg-1. The mean value of 137Cs activity concentration in the stomach wall was 23.7 Bq·kg-1. The mutual correlation was r = 0.988. There was further correlation of 137Cs activity concentration between the heart and the diaphragm (r = 0.977), the heart and the tongue (r = 0.902), and the heart and the lungs (r = 0.906). Based on the results from Slovakia and the Czech Republic, there is hope that it will be possible to use a sample from the stomach wall to determine the activity concentration of 137Cs in meat, thereby replacing the commonly used and highly valued top side sample. In practice, monitoring soil contamination to predict the meat contamination of wild boars is more important than monitoring elements of the food chain. The potassium-caesium coefficient reached 5.57 in the muscles and 17.25 in the stomach wall.","PeriodicalId":7192,"journal":{"name":"Acta Veterinaria Brno","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69208433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. Vodička, J. Pytlík, M. Stádníková, L. Stádník, J. Ducháček, R. Codl, Zuzana Biniová
The aim of this study was to evaluate the effects of 3 different commercially available extenders – AndroMed® (soy lecithin-based), Optidyl® (with the addition of ionized egg yolk), and BULLXcell® (with the addition of fresh egg yolk) – on bull spermatozoa quality, which was evaluated using the parameters of spermatozoa motility, viability, plasma membrane damage, acrosome damage, and mitochondrial activity after thawing and during a 2 h long thermoresistance test. The spermatozoa quality indicators were appraised by computer-assisted semen analysis and a flow cytometer. Significant differences (P < 0.01) between bulls were registered in all indicators measured. The highest average values of spermatozoa total motility and viability were achieved using BULLXcell® extender (44.33%; 52.06%). Variances in comparing this extender with Optidyl® and AndroMed® were −0.83%, −2.64%; −8.33%, −9.51%. The differences found between the egg yolk-based diluents (BULLXcell® and Optidyl®) and AndroMed® were significant (P < 0.01). Therefore, the more valuable extenders for bull semen dilution were egg yolk-based extenders, which provided higher post-thaw spermatozoa survival and quality than the soy lecithin-based extender.
{"title":"The effects of egg yolk-based and egg yolk-free diluents on the post-thaw quality of bull spermatozoa","authors":"J. Vodička, J. Pytlík, M. Stádníková, L. Stádník, J. Ducháček, R. Codl, Zuzana Biniová","doi":"10.2754/avb202291040339","DOIUrl":"https://doi.org/10.2754/avb202291040339","url":null,"abstract":"The aim of this study was to evaluate the effects of 3 different commercially available extenders – AndroMed® (soy lecithin-based), Optidyl® (with the addition of ionized egg yolk), and BULLXcell® (with the addition of fresh egg yolk) – on bull spermatozoa quality, which was evaluated using the parameters of spermatozoa motility, viability, plasma membrane damage, acrosome damage, and mitochondrial activity after thawing and during a 2 h long thermoresistance test. The spermatozoa quality indicators were appraised by computer-assisted semen analysis and a flow cytometer. Significant differences (P < 0.01) between bulls were registered in all indicators measured. The highest average values of spermatozoa total motility and viability were achieved using BULLXcell® extender (44.33%; 52.06%). Variances in comparing this extender with Optidyl® and AndroMed® were −0.83%, −2.64%; −8.33%, −9.51%. The differences found between the egg yolk-based diluents (BULLXcell® and Optidyl®) and AndroMed® were significant (P < 0.01). Therefore, the more valuable extenders for bull semen dilution were egg yolk-based extenders, which provided higher post-thaw spermatozoa survival and quality than the soy lecithin-based extender.","PeriodicalId":7192,"journal":{"name":"Acta Veterinaria Brno","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69208827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. Komprda, Vendula Popelková, Ludmila Kosaristanova, V. Šmídová
The objective of the study was to design, synthetize and characterize poly(lactic-co-glycolic) acid (PLGA) nanoparticles (NPs) with entrapped fish oil (FO) for possible application in a cutaneous wound healing. Morphology of NPs was evaluated by transmission electron microscopy. Antimicrobial characteristics were tested using the disk diffusion method and plate count method, and cytotoxicity was evaluated by the MTT assay. Fish oil (y) was released from PLGA NPs within the time interval (x) of 96 h according to equation y = 6.2 + 0.914x. PLGA-FO NPs did not affect growth of Staphylococcus aureus or methicillin-resistant S. aureus (MRSA) strains. No cytotoxic effect of the tested NPs on the keratinocyte cell line was observed for concentration of 1 µg/ml. PLGA-FO NPs represent an interesting alternative for wound healing due to an excellent biocompatibility and unique release profile of FO, despite their lack of antimicrobial efficiency.
{"title":"Poly(lactic-co-glycolic) acid nanoparticles as a delivery system for fish oil in wound healing","authors":"T. Komprda, Vendula Popelková, Ludmila Kosaristanova, V. Šmídová","doi":"10.2754/avb202291030285","DOIUrl":"https://doi.org/10.2754/avb202291030285","url":null,"abstract":"The objective of the study was to design, synthetize and characterize poly(lactic-co-glycolic) acid (PLGA) nanoparticles (NPs) with entrapped fish oil (FO) for possible application in a cutaneous wound healing. Morphology of NPs was evaluated by transmission electron microscopy. Antimicrobial characteristics were tested using the disk diffusion method and plate count method, and cytotoxicity was evaluated by the MTT assay. Fish oil (y) was released from PLGA NPs within the time interval (x) of 96 h according to equation y = 6.2 + 0.914x. PLGA-FO NPs did not affect growth of Staphylococcus aureus or methicillin-resistant S. aureus (MRSA) strains. No cytotoxic effect of the tested NPs on the keratinocyte cell line was observed for concentration of 1 µg/ml. PLGA-FO NPs represent an interesting alternative for wound healing due to an excellent biocompatibility and unique release profile of FO, despite their lack of antimicrobial efficiency.","PeriodicalId":7192,"journal":{"name":"Acta Veterinaria Brno","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69208899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Ateya, H. Ghanem, Mona M. Elghareeb, U. Abou-Ismail, B. Hendam
The objective of this study was to investigate polymorphisms of DGAT1, FABP, OLR1 and ATP1A1 genes using PCR-DNA sequencing, and to associate these genetic structures to changes in metabolic biochemical markers and milk composition indicators in a total of 90 dairy cows of the Holstein, Simmental, and Brown Swiss breeds (30 cows each). PCR was carried out for amplification of 411-bp of DGAT1, 525-bp of FABP, 582-bp of OLR1, and 300-bp of ATP1A1 genes. Three breeds’ nucleotide sequence variations in the form of single nucleotide polymorphisms (SNPs) were detailed by DNA sequencing analysis. Chisquare analysis showed that the distribution of all discovered SNPs varied significantly (P < 0.001). Biochemical indices in cow’s serum revealed no significant difference in serum total protein, albumin, and total cholesterol among the three breeds. However, triglyceride showed a significant increase in Simmental compared to either Holsteins or Brown Swiss, while the highest mean value of triiodothyronine (T3) and tetraiodothyronine (T4) was detected in Holstein dairy cows The milk composition indicators analysis revealed that milk protein, sugar, and density were significantly higher in Holsteins than both Simmental and Brown Swiss. Meanwhile, milk fat and total solids revealed a significantly higher increase in Simmental than both brown Swiss and Holstein. As a result, the metabolic biochemical markers profile along with the identified SNPs could be used as a candidate and a reference guide for effective characterization of the Holstein, Simmental, and Brown Swiss breeds, leading to the creation of a marker-assisted selection system for production traits in dairy cattle breeds.
{"title":"Single nucleotide polymorphisms and metabolic biochemical profile of productive markers characterize three European breeds of dairy cattle","authors":"A. Ateya, H. Ghanem, Mona M. Elghareeb, U. Abou-Ismail, B. Hendam","doi":"10.2754/avb202291040317","DOIUrl":"https://doi.org/10.2754/avb202291040317","url":null,"abstract":"The objective of this study was to investigate polymorphisms of DGAT1, FABP, OLR1 and ATP1A1 genes using PCR-DNA sequencing, and to associate these genetic structures to changes in metabolic biochemical markers and milk composition indicators in a total of 90 dairy cows of the Holstein, Simmental, and Brown Swiss breeds (30 cows each). PCR was carried out for amplification of 411-bp of DGAT1, 525-bp of FABP, 582-bp of OLR1, and 300-bp of ATP1A1 genes. Three breeds’ nucleotide sequence variations in the form of single nucleotide polymorphisms (SNPs) were detailed by DNA sequencing analysis. Chisquare analysis showed that the distribution of all discovered SNPs varied significantly (P < 0.001). Biochemical indices in cow’s serum revealed no significant difference in serum total protein, albumin, and total cholesterol among the three breeds. However, triglyceride showed a significant increase in Simmental compared to either Holsteins or Brown Swiss, while the highest mean value of triiodothyronine (T3) and tetraiodothyronine (T4) was detected in Holstein dairy cows The milk composition indicators analysis revealed that milk protein, sugar, and density were significantly higher in Holsteins than both Simmental and Brown Swiss. Meanwhile, milk fat and total solids revealed a significantly higher increase in Simmental than both brown Swiss and Holstein. As a result, the metabolic biochemical markers profile along with the identified SNPs could be used as a candidate and a reference guide for effective characterization of the Holstein, Simmental, and Brown Swiss breeds, leading to the creation of a marker-assisted selection system for production traits in dairy cattle breeds.","PeriodicalId":7192,"journal":{"name":"Acta Veterinaria Brno","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69209204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
František Kouba, K. Vernerová, M. Šoch, V. Hanzal, Lucie Filásová, Z. Semerád, F. Svoboda, Jan Rosmus
The monitoring of radiocaesium in the ecosystems of particular areas in the Czech Republic is necessary even decades following the Chernobyl nuclear disaster. We report an evaluation of the radioactive contamination of the meat of wild boars hunted in the Novohradské (Gratzen) Mountains in the period of December 2012 to December 2019. Radionuclide 137Cs is surveyed for food safety as a source of food chain contamination and a possible risk for human health. Overall, 654 samples of game meat from wild boars were analysed for 137Cs activity. The 137Cs legal limit of 600 Bq·kg-1 was exceeded in 238 samples (36.4%) of hunted wild boar meat. Statistical analysis showed that 137Cs activities measured in wild boar muscle in March reached lower values compared to the period from June till November (P < 0.05). Higher values were reported in September (P < 0.05) as well as in August (P < 0.01) compared to December. A significant season-based 137Cs level was found (P < 0.000). Higher 137Cs activities in wild boar in the winter season (November to April) are related to decreasing access to a naturally occurring diet with a lower 137Cs content. The keepers of the wild boar in this area were ordered to comply with obligatory emergency veterinary precautions set by the Regional Veterinary Administration to analyse all hunted game meat for radiocaesium. All wild boars with an above-limit 137Cs value had to be excluded from the food chain by hunting ground keepers and disposed of safely.
{"title":"Radiocaesium in wild boars in Novohradské (Gratzen) Mountains","authors":"František Kouba, K. Vernerová, M. Šoch, V. Hanzal, Lucie Filásová, Z. Semerád, F. Svoboda, Jan Rosmus","doi":"10.2754/avb202291010087","DOIUrl":"https://doi.org/10.2754/avb202291010087","url":null,"abstract":"The monitoring of radiocaesium in the ecosystems of particular areas in the Czech Republic is necessary even decades following the Chernobyl nuclear disaster. We report an evaluation of the radioactive contamination of the meat of wild boars hunted in the Novohradské (Gratzen) Mountains in the period of December 2012 to December 2019. Radionuclide 137Cs is surveyed for food safety as a source of food chain contamination and a possible risk for human health. Overall, 654 samples of game meat from wild boars were analysed for 137Cs activity. The 137Cs legal limit of 600 Bq·kg-1 was exceeded in 238 samples (36.4%) of hunted wild boar meat. Statistical analysis showed that 137Cs activities measured in wild boar muscle in March reached lower values compared to the period from June till November (P < 0.05). Higher values were reported in September (P < 0.05) as well as in August (P < 0.01) compared to December. A significant season-based 137Cs level was found (P < 0.000). Higher 137Cs activities in wild boar in the winter season (November to April) are related to decreasing access to a naturally occurring diet with a lower 137Cs content. The keepers of the wild boar in this area were ordered to comply with obligatory emergency veterinary precautions set by the Regional Veterinary Administration to analyse all hunted game meat for radiocaesium. All wild boars with an above-limit 137Cs value had to be excluded from the food chain by hunting ground keepers and disposed of safely.","PeriodicalId":7192,"journal":{"name":"Acta Veterinaria Brno","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69208673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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