Engineered regeneration最新文献

英文中文

Q1 Medicine

Engineered regeneration

Pub Date : 2025-01-01

引用次数: 0

Q1 Medicine

Engineered regeneration

Pub Date : 2025-01-01

引用次数: 0

Q1 Medicine

Engineered regeneration

Pub Date : 2025-01-01

引用次数: 0

Q1 Medicine

Engineered regeneration

Pub Date : 2025-01-01

引用次数: 0

Corrigendum to “The Artificial Disc Nucleus and Other Strategies for Replacement of the Nucleus Pulposus: Past, Present and Future Designs for an Emerging Surgical Solution” [Engineered Regeneration 5(2024), 269-281] “人工椎间盘核和其他替代髓核的策略：一种新兴手术解决方案的过去、现在和未来设计”的勘误表[工程再生5(2024),269-281]

Q1 Medicine

Engineered regeneration

Pub Date : 2024-12-01 DOI: 10.1016/j.engreg.2024.12.001

Greg Sacks, Vincent DeStefano, Claire Parker, Ryan Lebens, Harry Mushlin

引用次数: 0

Asymmetric porous composite hydrogel patch for microenvironment-adapted repair of contaminated abdominal wall defects 非对称多孔复合水凝胶补片用于微环境适应性修复污染性腹壁缺损

Q1 Medicine

Engineered regeneration

Pub Date : 2024-12-01 DOI: 10.1016/j.engreg.2024.03.004

Yang Yu , Yinxiang Tang , Weiwen Liang , Yuanbin Wang , Yang Ouyang , Wenxuan Xiong , Bingna Zheng , Lili Chu , Hui Wang

Effective antibacterial property and long-term mechanical support are essential for the repair of complex abdominal wall defects associated with infection. However, clinically available repair materials often fail to meet these requirements, resulting in high surgical failure rate and complications. In this study, an asymmetric porous composite hydrogel patch (cCS/PVA@BAC) with antibacterial, anti-adhesion, pro-healing, and durable mechanical support properties is designed for the efficient repair of contaminated abdominal wall defects. By stepwise phase-conversion and soaking method, robust and stable polyvinyl alcohol hydrogel (PVAH) is integrated with the biocompatible multicomponent hydrogel made of chitosan and carboxymethyl chitosan (cCS), and benzalkonium chloride (BAC) is loaded to enhance the antibacterial property. The cCS layer of cCS/PVA@BAC has an extracellular matrix-like porous structure, which can promote fibroblasts adhesion and wound healing. In contrast, the PVAH layer on the other side with a smooth and dense structure, which can reduce fibroblasts adhesion and prevent visceral adhesion. In addition, the composite hydrogel patch has good anti-swelling and anti-deformation properties as well as stable mechanical strength, thus can withstand high intraperitoneal pressure in the wet internal microenvironment. The loaded BAC can efficiently kill bacteria and improve the local inflammatory microenvironment. With these advantages, cCS/PVA@BAC can significantly reduce inflammation, promote tissue remodeling, and accelerate the healing of contaminated abdominal wall defects in the rat model. These findings suggest a potential use of multifunctional hydrogel patch as an ideal material for effective repair of contaminated soft tissue defects.

有效的抗菌性能和长期的机械支持是修复复杂的腹壁缺损与感染的必要条件。然而，临床上可用的修复材料往往不能满足这些要求，导致高手术失败率和并发症。本研究设计了一种非对称多孔复合水凝胶贴片（cCS/PVA@BAC），具有抗菌、抗粘连、促进愈合和耐用的机械支撑性能，可用于污染腹壁缺陷的有效修复。通过逐步相转化和浸渍法，将稳定的聚乙烯醇水凝胶（PVAH）与壳聚糖和羧甲基壳聚糖（cCS）制备的生物相容性多组分水凝胶（PVAH）相结合，并加载苯甲氯铵（BAC）增强其抗菌性能。cCS/PVA@BAC的cCS层具有细胞外基质样多孔结构，能促进成纤维细胞粘附和伤口愈合。而另一侧PVAH层结构光滑致密，可减少成纤维细胞粘连，防止内脏粘连。此外，复合水凝胶贴片具有良好的抗膨胀、抗变形性能和稳定的机械强度，可以在潮湿的内部微环境中承受较高的腹腔压力。负载BAC能有效杀灭细菌，改善局部炎症微环境。由于这些优点，cCS/PVA@BAC在大鼠模型中可以显著减少炎症，促进组织重塑，加速污染腹壁缺损的愈合。这些发现提示多功能水凝胶贴片作为一种理想的材料，可以有效地修复受污染的软组织缺损。

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引用次数: 0

Bone improvement in osteoporotic rabbits using CoCrMo implants 使用钴铬钼合金植入体改善骨质疏松兔子的骨质

Q1 Medicine

Engineered regeneration

Pub Date : 2024-12-01 DOI: 10.1016/j.engreg.2024.05.002

Jésica I. Zuchuat , Adriana S. Manzano , Valeria Sigot , Gastón L. Miño , Oscar A. Decco

The management of bone repair in patients with osteoporosis depends on the clinical situation and the extent of the damage. The repair of bone lesions by inducing new bone formation is important for maintaining bone architecture and density. Herein, we reported the use of Cobalt Chromium Molybdenum (CoCrMo) implants in osteoporotic rabbits and the regenerative outcomes in vivo. The aim was to determine whether the placement of CoCrMo plates would induce qualitative and quantitative differences in the osteoporotic tissue beneath and surrounding the implant. We assessed the effect of the alloy in the bone of animals receiving implants for 4 and 8 weeks and compared the results to those of the osteoporotic non-implanted bone and the healthy controls. After 4 weeks, minimal histological changes were observed, whereas after 8 weeks a marked osteogenesis was evident with both apposition and substitution of new bone. In addition, a greater number of Haversian canals with increased canal area and decreased intracortical pores were observed in the implanted vs non implanted limb for both experimental groups. We show for the first time that the use of CrCoMo plates induces bone formation under osteoporotic conditions. The beneficial effect is localised on the cortical bone in areas in contact with the material. Although this effect may not directly influence the OP disease itself, it has direct implications for new bone formation adjacent to the biomaterial. This potential enhancement could play a crucial role in improving implant fixation in compromised bone, offering increased biocompatibility and stability.

骨质疏松患者的骨修复处理取决于临床情况和损伤程度。通过诱导新骨形成来修复骨损伤对于维持骨结构和密度是重要的。在此，我们报道了钴铬钼（CoCrMo）植入物在骨质疏松兔子体内的使用和再生结果。目的是确定CoCrMo板的放置是否会引起种植体下方和周围骨质疏松组织的定性和定量差异。我们评估了合金在接受植入物4周和8周的动物骨骼中的作用，并将结果与骨质疏松的未植入骨和健康对照进行了比较。4周后，观察到最小的组织学变化，而8周后，随着新骨的附着和替代，明显的成骨。此外，与未植入肢体相比，植入肢体的哈弗斯管数量更多，管面积增加，皮质内孔减少。我们首次证明在骨质疏松的情况下使用CrCoMo钢板诱导骨形成。这种有益作用局限于与材料接触区域的皮质骨。虽然这种作用可能不会直接影响OP疾病本身，但它对生物材料附近的新骨形成有直接影响。这种潜在的增强可能在改善受损骨的种植体固定方面发挥关键作用，提供更高的生物相容性和稳定性。

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引用次数: 0

Polyphenol-based photothermal nanoparticles with sprayable capability for self-regulation of microenvironment to accelerate diabetic wound healing 基于多酚的光热纳米颗粒具有可喷洒功能，可自我调节微环境以加速糖尿病伤口愈合

Q1 Medicine

Engineered regeneration

Pub Date : 2024-12-01 DOI: 10.1016/j.engreg.2024.05.003

Xiuhong Huang , Meimei Fu , Min Lu , Xiaoxian Wu , Weiqian David Hong , Xiaoying Wang , Panpan Wu , Keke Wu

Current treatments for diabetic wounds have some curative effect, but the process is complicated and lack user-friendly wound dressings. Nanozymes have gained significant attention for wound healing due to their striking merits. Herein, we have developed a novel sprayable tannin acid-cobalt coordination nanozyme (TACo) for diabetic wound healing. TACo nanozyme offers a convenient and efficient methods by spraying directly onto wounds surface, reducing infection risk by avoiding direct contact. Notably, its antioxidant properties contribute to scavenging the reactive oxygen species (ROS), alleviating oxidative stress and inflammation of wound microenvironment. Additionally, TACo nanozyme could promote cell survival and multiplication, which is crucial for the wound healing process. Importantly, TACo nanozyme facilitates angiogenesis by enhancing cell viability, migration, and tube formation. The unique coordination between metal and phenolic components confers pH-responsive cobalt ion and TA release properties, avoiding secondary damage during the wound cleaning. This unique composition seamlessly integrates photothermal antibacterial therapy, inflammatory microenvironment management, supporting for angiogenesis, and effective promotion of extracellular matrix production sequentially by harnessing the acidic pH environment of diabetic wounds. In conclusion, the development of a sprayable TACo nanozyme presents a promising therapeutic approach for the treatment of diabetic wounds, addressing the complexities of current treatments and providing a user-friendly application method.

目前对糖尿病创面的治疗虽有一定疗效，但治疗过程复杂且缺乏方便使用的创面敷料。纳米酶由于其显著的优点，在伤口愈合方面受到了极大的关注。在此，我们开发了一种用于糖尿病伤口愈合的新型喷雾单宁酸钴配位纳米酶（TACo）。TACo纳米酶提供了方便和有效的方法，直接喷洒在伤口表面，避免直接接触，降低感染风险。值得注意的是，其抗氧化特性有助于清除活性氧（ROS），减轻伤口微环境的氧化应激和炎症。此外，TACo纳米酶可以促进细胞存活和增殖，这对伤口愈合过程至关重要。重要的是，TACo纳米酶通过增强细胞活力、迁移和管形成来促进血管生成。金属和酚类成分之间的独特配合赋予了ph响应钴离子和TA释放特性，避免了伤口清洁过程中的二次损伤。这种独特的成分无缝集成光热抗菌治疗，炎症微环境管理，支持血管生成，并通过利用糖尿病伤口的酸性pH环境有效促进细胞外基质的生产。总之，一种可喷雾的TACo纳米酶的开发为糖尿病伤口的治疗提供了一种有前景的治疗方法，解决了当前治疗的复杂性，并提供了一种用户友好的应用方法。

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引用次数: 0

Bis-5HT modified Paris Saponin II nanoparticles treat neutrophil infiltrating allergic rhinitis by regulating the alanine metabolism pathway Bis-5HT修饰的巴黎皂苷II纳米颗粒通过调节丙氨酸代谢途径治疗中性粒细胞浸润性变应性鼻炎

Q1 Medicine

Engineered regeneration

Pub Date : 2024-12-01 DOI: 10.1016/j.engreg.2024.02.004

Wenwen Qi , Lei Shi , Xinhao Wu , Fangyuan Zhu , Zhenxiao Teng , Xiaochen Gao , Xin Bing , Na Guo , Xue Cao , Chengzhilin Li , Houyang Hu , Fanyu Yuan , Yuhan Dong , Ming Xia , Chengcheng Liu

Allergic rhinitis (AR) has emerged as a global concern, particularly due to the recent rise in disease incidence. There is an urgent need for safer, more effective, and shorter-term targeted immunotherapy approaches. Our previous studies have demonstrated the potential of paris saponins II in mitigating neutrophil infiltration in the nasal mucosa of AR mice. However, its clinical applicability has been hampered by limited by bio availability and bioactivity. In response to these limitations, we have developed bis-5HT-modified paris saponins II (designated as PLGA-5HT-PSII-Ce6) to target neutrophil-specific myeloperoxidase. Our verification, using metabolomics and other techniques, has affirmed the enhanced therapeutic efficacy of this targeted drug for allergic rhinitis. Furthermore, the incorporation of photosensitizers has improved the treatment effect particularly when light induction is introduced. This development lead to promising prospects for the treatment of AR.

过敏性鼻炎（AR）已成为全球关注的问题，特别是由于最近疾病发病率的上升。迫切需要更安全、更有效、更短期的靶向免疫治疗方法。我们之前的研究已经证明了巴黎皂苷II在减轻AR小鼠鼻黏膜中性粒细胞浸润方面的潜力。然而，其临床应用受到生物利用度和生物活性的限制。针对这些局限性，我们开发了双- 5ht修饰的巴黎皂苷II（命名为PLGA-5HT-PSII-Ce6）来靶向中性粒细胞特异性髓过氧化物酶。我们利用代谢组学等技术进行验证，证实了该靶向药物对变应性鼻炎的治疗效果增强。此外，光敏剂的掺入改善了处理效果，特别是当引入光感应时。这一进展为AR的治疗带来了广阔的前景。

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引用次数: 0

Novel injectable composite incorporating denosumab promotes bone regeneration via bone homeostasis regulation 含有地诺单抗的新型可注射复合材料通过调节骨平衡促进骨再生

Q1 Medicine

Engineered regeneration

Pub Date : 2024-12-01 DOI: 10.1016/j.engreg.2024.05.001

Guang Yang , Zili Guo , Xiangfeng Zhang , Jiayu Chen , Jie Weng , Jiapeng Bao , Xiaohua Yu

Repair of large bone defects remains to be clinically challenging, yet current bone repair strategies focus on optimizing the osteogenic capacity of bone grafts, while the role of osteoclasts in bone regeneration has been largely ignored. Herein, we designed a injectable self-curing bone grafting paste capable of regulating both anabolic/catabolic activities during bone healing by immobilizing the RANKL inhibitor denosumab on dermal-derived extracellular matrix (ECM) microfibres, which were then incorporated into an injectable paste via a hydration reaction between β-tricalcium phosphate (β-TCP), monocalcium phosphate monohydrate (MCPM) and calcium sulfate hemihydrate (CSH). The incorporation of ECM microfibres not only serves as a sustained-release denosumab carrier to inhibit osteoclastogenesis but also improves the mechanical properties of the resulting composite by increasing the interaction between the organic and inorganic phases. In vitro, calcium supply from the composite along with ECM enhanced osteogenic differentiation of BMSC while release of denosumab effectively inhibits osteoclast fusion and alleviate osteoclastic activity. In vivo, it was observed that CSH/CP@ECM-Deno significantly reduced the number of osteoclasts, slowed down the process of bone resorption, and accelerated collagen deposition to promote new bone generation. These results suggest that modulation of osteoclastogenesis by interfering with bone homeostasis may be an effective bone repair strategy.

大面积骨缺损的修复在临床上仍具有挑战性，但目前的骨修复策略主要集中在优化骨移植物的成骨能力，而破骨细胞在骨再生中的作用在很大程度上被忽视。在此，我们设计了一种可注射的自固化植骨膏，通过将RANKL抑制剂denosumab固定在真皮来源的细胞外基质（ECM）微纤维上，能够调节骨愈合过程中的合成代谢/分解代谢活动，然后通过β-磷酸三钙（β-TCP）、磷酸一钙一水（MCPM）和半水合硫酸钙（CSH）之间的水化反应将其掺入可注射的植骨膏中。ECM微纤维的掺入不仅可以作为一种缓释的denosumab载体来抑制破骨细胞的发生，而且还可以通过增加有机相和无机相之间的相互作用来改善所得到的复合材料的机械性能。在体外，复合材料的钙供应和ECM增强了BMSC的成骨分化，而denosumab的释放有效抑制破骨细胞融合并减轻破骨细胞活性。在体内，我们观察到CSH/CP@ECM-Deno显著减少破骨细胞数量，减缓骨吸收过程，加速胶原沉积，促进新骨生成。这些结果表明，通过干扰骨稳态来调节破骨细胞的发生可能是一种有效的骨修复策略。

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