Pub Date : 2021-10-31DOI: 10.24018/ejbio.2021.2.5.235
B. Salkić, E. Salkić, Amela Hercegovac, A. Avdić, Azra Dorić, A. Salkić
Pear as a fruit species has a special place because of its quality characteristic. The fact that about 20,000 pear seedlings are considered to be sold annually in Bosnia and Herzegovina, which are autochthonous or spontaneously expanded varieties, also speaks in favor. The main goal of this research is to analyze the genetic variability of nine autochthonous pear varieties in Tuzla Canton, Bosnia and Herzegovina to enable the conservation and expansion of existing genetic resources. The study included nine autochthonous pear varieties. Samples of young leaves were collected on the following localities: the town of Srebrenik, the town of Gradačac, the municipality of Čelić-Tuzla Canton, Bosnia and Herzegovina, in the spring 2019. To determine genetic diversity, 12 SSR (Simple Sequence Repeats) markers were genotyped. There were no cases of synonyms or homonyms in the analyzed set. Each of the nine tested varieties represents a unique genotype. Autochthonous pear cultivars analyzed in this paper represent an interesting genetic resource, with useful agronomic traits that can be used in future cultivation.
{"title":"Genetic Variability of Autochthonous Pear Varieties from Tuzla Canton, Bosnia and Herzegovina","authors":"B. Salkić, E. Salkić, Amela Hercegovac, A. Avdić, Azra Dorić, A. Salkić","doi":"10.24018/ejbio.2021.2.5.235","DOIUrl":"https://doi.org/10.24018/ejbio.2021.2.5.235","url":null,"abstract":"Pear as a fruit species has a special place because of its quality characteristic. The fact that about 20,000 pear seedlings are considered to be sold annually in Bosnia and Herzegovina, which are autochthonous or spontaneously expanded varieties, also speaks in favor. The main goal of this research is to analyze the genetic variability of nine autochthonous pear varieties in Tuzla Canton, Bosnia and Herzegovina to enable the conservation and expansion of existing genetic resources. The study included nine autochthonous pear varieties. Samples of young leaves were collected on the following localities: the town of Srebrenik, the town of Gradačac, the municipality of Čelić-Tuzla Canton, Bosnia and Herzegovina, in the spring 2019. To determine genetic diversity, 12 SSR (Simple Sequence Repeats) markers were genotyped. There were no cases of synonyms or homonyms in the analyzed set. Each of the nine tested varieties represents a unique genotype. Autochthonous pear cultivars analyzed in this paper represent an interesting genetic resource, with useful agronomic traits that can be used in future cultivation.","PeriodicalId":72969,"journal":{"name":"European journal of biology and biotechnology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68819810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-24DOI: 10.24018/ejbio.2021.2.5.297
Ghada Shebl, H. Sayed-Ahmed, Mohamed Bassuony Hamza, Ashraf Salah El-Din Haider
Dopamine is a neurotransmitter hormone for pleasure and reward. It is synthesized only in the brain cells of human and animals and responsible for the regulation of behavior, mood, memory, cognitive, flexible movement, the body weight, and other important functions. The Dopamine Receptor D3 (DRD3) is the most important receptor for dopamine. In this investigation, expression of DRD3 gene was studied in rabbits fed on supplemented diet of dry and fresh faba bean (Vicia faba L. Sakha 3). DRD3 gene (≈ 1200 bp) in control and treated rabbits were PCR amplified, sequenced and aligned with reference gene (Acc. No XM_017346708.1). High genetic similarity values were detected among all sequences. DRD3 gene sequences of control, fresh and dry faba bean fed rabbits were deposited in the GenBank with accession numbers MZ714134, MZ714135 and MZ714136 respectively. Direct estimation of blood phenylalanine (Phe) amino acid indicated that feeding rabbits on dry faba bean reflected the highest level of Phe in the rabbit’s blood. Quantitative RT-qPCR analysis showed that DRD3 gene was over expressed after feeding rabbits on dry faba bean form compared with feeding on green form and control. Thus, diet rich with phenylalanine like Sakha3 (dry and fresh forms) enhance gene expression of DRD3 gene. However, diet doesn't affect the DRD3 gene sequence and structure. In a conclusion, our findings indicated a direct effect of faba bean supplemented diet on increasing DRD3 expression levels which improve the life quality for human.
{"title":"Phenylalanine Rich Diet (Vicia faba L.) Enhances the Expression of Dopamine Receptor D3 (DRD3) Gene in Rabbits","authors":"Ghada Shebl, H. Sayed-Ahmed, Mohamed Bassuony Hamza, Ashraf Salah El-Din Haider","doi":"10.24018/ejbio.2021.2.5.297","DOIUrl":"https://doi.org/10.24018/ejbio.2021.2.5.297","url":null,"abstract":"Dopamine is a neurotransmitter hormone for pleasure and reward. It is synthesized only in the brain cells of human and animals and responsible for the regulation of behavior, mood, memory, cognitive, flexible movement, the body weight, and other important functions. The Dopamine Receptor D3 (DRD3) is the most important receptor for dopamine. In this investigation, expression of DRD3 gene was studied in rabbits fed on supplemented diet of dry and fresh faba bean (Vicia faba L. Sakha 3). DRD3 gene (≈ 1200 bp) in control and treated rabbits were PCR amplified, sequenced and aligned with reference gene (Acc. No XM_017346708.1). High genetic similarity values were detected among all sequences. DRD3 gene sequences of control, fresh and dry faba bean fed rabbits were deposited in the GenBank with accession numbers MZ714134, MZ714135 and MZ714136 respectively. Direct estimation of blood phenylalanine (Phe) amino acid indicated that feeding rabbits on dry faba bean reflected the highest level of Phe in the rabbit’s blood. Quantitative RT-qPCR analysis showed that DRD3 gene was over expressed after feeding rabbits on dry faba bean form compared with feeding on green form and control. Thus, diet rich with phenylalanine like Sakha3 (dry and fresh forms) enhance gene expression of DRD3 gene. However, diet doesn't affect the DRD3 gene sequence and structure. In a conclusion, our findings indicated a direct effect of faba bean supplemented diet on increasing DRD3 expression levels which improve the life quality for human.","PeriodicalId":72969,"journal":{"name":"European journal of biology and biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43712234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-22DOI: 10.24018/ejbio.2021.2.5.286
Z. Rakotovao-Ravahatra, L. Rahajamanana, L. Rakotondraoelina, L. Raskine, S. Rasoanandrasana, C. Rafalimanana, R. Rakotomalala, Arinomenjanahary Rakotoarisoa, Lalatiana Valisoa Andriambelo, Hoby Tantelinirimiarana, Jocia Fenomanana, Irène Rakotoniaina, Z. Ravaoarisaina, A. C. Razafindrakoto, Tsiriniaina Ramavoson, Eddy Ramaminiaina, Heritiana Randrianary, Andry Tiana Razafinikasa, Jean Emile Ravelomandranto, A. L. Rakotovao
In medical analysis laboratories, techniques for identifying bacteria are currently becoming more and more numerous. The objective of this study is to compare the 2 bacterial identification systems Bis NEG-D and Api 20E for the identification of gram-negative bacilli. This is a qualitative evaluation of the Bis NEG-D compared to the gold standard Api 20E. During the study period, 32 Gram-negative bacilli isolates were identified simultaneously using Api 20E and Bis NEG-D. The samples are represented by 12 (37.5%) blood samples for blood culture, 12 (37.5%) urine samples for cytobacteriological examination of the urine, 06 (18.8%) pus samples for bacteriological examination of pus, a sample of the cerebrospinal fluid (3.1%) and a vaginal sample (3.1%).The bacteria identified were represented by Enterobacter spp, Escherichia col,i Klebsiella pneumonia, Shigella spp, Salmonella typhi, Acinetobacter baumannii, Pseudomonas spp, Proteus mirabilis, Raoultella ornithinolytica and Bukholderia cepacia. This study showed a concordance of 90.6% (29/32) and a discordance of 9.4% (3/32) between the results of Api 20E and Bis NEG-D. Concerning the probability scores, they vary between 95% to 100% for Api 20E and between 79.3% to 100% for Bis NEG-D. This study also compared the pros and cons of using Api 20 E and Bis NEG-D. The Bis-NEG-D is valid and can be used by medical analysis laboratories like the Api 20E, especially if these laboratories do not need to perform a lot of bacterial identification tests.
{"title":"Comparison of Bis NEG-D and API 20E for the Identification of Gram-negative Bacilli in the Laboratory of the University Hospital of Befelatanana Antananarivo Madagascar","authors":"Z. Rakotovao-Ravahatra, L. Rahajamanana, L. Rakotondraoelina, L. Raskine, S. Rasoanandrasana, C. Rafalimanana, R. Rakotomalala, Arinomenjanahary Rakotoarisoa, Lalatiana Valisoa Andriambelo, Hoby Tantelinirimiarana, Jocia Fenomanana, Irène Rakotoniaina, Z. Ravaoarisaina, A. C. Razafindrakoto, Tsiriniaina Ramavoson, Eddy Ramaminiaina, Heritiana Randrianary, Andry Tiana Razafinikasa, Jean Emile Ravelomandranto, A. L. Rakotovao","doi":"10.24018/ejbio.2021.2.5.286","DOIUrl":"https://doi.org/10.24018/ejbio.2021.2.5.286","url":null,"abstract":"In medical analysis laboratories, techniques for identifying bacteria are currently becoming more and more numerous. The objective of this study is to compare the 2 bacterial identification systems Bis NEG-D and Api 20E for the identification of gram-negative bacilli. This is a qualitative evaluation of the Bis NEG-D compared to the gold standard Api 20E. During the study period, 32 Gram-negative bacilli isolates were identified simultaneously using Api 20E and Bis NEG-D. The samples are represented by 12 (37.5%) blood samples for blood culture, 12 (37.5%) urine samples for cytobacteriological examination of the urine, 06 (18.8%) pus samples for bacteriological examination of pus, a sample of the cerebrospinal fluid (3.1%) and a vaginal sample (3.1%).The bacteria identified were represented by Enterobacter spp, Escherichia col,i Klebsiella pneumonia, Shigella spp, Salmonella typhi, Acinetobacter baumannii, Pseudomonas spp, Proteus mirabilis, Raoultella ornithinolytica and Bukholderia cepacia. This study showed a concordance of 90.6% (29/32) and a discordance of 9.4% (3/32) between the results of Api 20E and Bis NEG-D. Concerning the probability scores, they vary between 95% to 100% for Api 20E and between 79.3% to 100% for Bis NEG-D. This study also compared the pros and cons of using Api 20 E and Bis NEG-D. The Bis-NEG-D is valid and can be used by medical analysis laboratories like the Api 20E, especially if these laboratories do not need to perform a lot of bacterial identification tests.","PeriodicalId":72969,"journal":{"name":"European journal of biology and biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42353930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-19DOI: 10.24018/ejbio.2021.2.5.278
H. Sultana, L. Nahar, M. Hossain, T. Ghosh, Md. Sanaullah Biswas
In vitro regeneration of cucumber is relatively difficult for genetic improvement. In this regard, different concentrations of growth regulators and three types of explants (cotyledon, hypocotyl and leaf disc) were investigated for their efficiency on callus induction potential. Among different explants explored for callus induction with different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D), leaf disc responded earlier (4.67 days) and showed higher percentage of callus induction (91.50%) with 2 mg/l 2,4-D supplemented Murashige and Skoog (MS) media. The same concentration of 2,4-D resulted in the maximum callus fresh (0.56 g) and dry weight (0.39 g) from leaf disc explant. Then the callus was transferred to untreated, 2.0 mg/l BAP + 0.2 mg/l NAA + 1.0 mg/l Kn, 2.0 mg/l BAP + 1.0 mg/l NAA + 1.0 mg/l Kn and 2.0 mg/l BAP + 1.5 mg/l NAA + 1.0 mg/l Kn fortified MS medium. After transferring the callus of different explants to shoot regeneration media containing different concentrations of 6-benzylaminopurine (BAP), 1-naphthaleneacetic acid (NAA) and Kinetin (Kn), only cotyledon callus started to regenerate shoot. The combination of BAP (2 mg/l) + NAA (0.2 mg/l) + Kn (1 mg/l) showed highest shoot regeneration percentage (67.77%) and the maximum number of shoots (5.12) per explant were recorded in the treatment combination of 2 mg/l BAP + 0.2 mg/l NAA + 1 mg/l Kn. These results provided a basis for the optimization of the callus induction protocol of cucumber for genetic transformation.
{"title":"Response of Different Explants for Callus Induction in Cucumber","authors":"H. Sultana, L. Nahar, M. Hossain, T. Ghosh, Md. Sanaullah Biswas","doi":"10.24018/ejbio.2021.2.5.278","DOIUrl":"https://doi.org/10.24018/ejbio.2021.2.5.278","url":null,"abstract":"In vitro regeneration of cucumber is relatively difficult for genetic improvement. In this regard, different concentrations of growth regulators and three types of explants (cotyledon, hypocotyl and leaf disc) were investigated for their efficiency on callus induction potential. Among different explants explored for callus induction with different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D), leaf disc responded earlier (4.67 days) and showed higher percentage of callus induction (91.50%) with 2 mg/l 2,4-D supplemented Murashige and Skoog (MS) media. The same concentration of 2,4-D resulted in the maximum callus fresh (0.56 g) and dry weight (0.39 g) from leaf disc explant. Then the callus was transferred to untreated, 2.0 mg/l BAP + 0.2 mg/l NAA + 1.0 mg/l Kn, 2.0 mg/l BAP + 1.0 mg/l NAA + 1.0 mg/l Kn and 2.0 mg/l BAP + 1.5 mg/l NAA + 1.0 mg/l Kn fortified MS medium. After transferring the callus of different explants to shoot regeneration media containing different concentrations of 6-benzylaminopurine (BAP), 1-naphthaleneacetic acid (NAA) and Kinetin (Kn), only cotyledon callus started to regenerate shoot. The combination of BAP (2 mg/l) + NAA (0.2 mg/l) + Kn (1 mg/l) showed highest shoot regeneration percentage (67.77%) and the maximum number of shoots (5.12) per explant were recorded in the treatment combination of 2 mg/l BAP + 0.2 mg/l NAA + 1 mg/l Kn. These results provided a basis for the optimization of the callus induction protocol of cucumber for genetic transformation.","PeriodicalId":72969,"journal":{"name":"European journal of biology and biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46722779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-11DOI: 10.24018/ejbio.2021.2.5.265
U. M. Askira, E. Marshall, S. A. Onyilokwu, A. Bukar, H. Balla, Hannatu K. Ali
Introduction: Malaria parasite is a protozoan disease that is transmitted by female anopheles mosquito which infects humans regardless of age, sex and status. It has a worldwide distribution and often prevalent in the developing countries and areas with poor environmental hygiene this study focuses on the incidence of plasmodiasis in relation to haematological parameters among children in the Internally Displaced Persons (IDPs) camp. Methods: blood samples were collected via venipuncture for thick blood film and was stained with giemsa diluted 1:10 and rapid diagnostic techniques (RDT), while the hematological parameter were analyzed by auto analyzer machine. Results: a total of two hundred and one (201) samples were obtained from two different camps in the study area. 87 were obtained from Stadium IDPs camp and 114 from Bakassi camp. From the Stadium IDPs camp 25(12.4%) were malaria positive and 62(30.8%) were negative. Similarly, from Bakassi IDPs camp, 58(28.9%) were positive and 56(27.9%) were negative. Conclusions: Males were shown to have eosinophilia compared to the females, due to an increase in the eosinophil count in them which can be used to predict the intensity of malaria infection, and a decrease in the eosinophil in females. There was a partial negative correlation due to a decrease in the monocyte and lymphocyte with increasing parasite density count.
{"title":"Plasmodiasis in Relation to Haematological Parameters among Children in the Internally Displaced Persons Camps (IDPS) within Maiduguri, Nigeria","authors":"U. M. Askira, E. Marshall, S. A. Onyilokwu, A. Bukar, H. Balla, Hannatu K. Ali","doi":"10.24018/ejbio.2021.2.5.265","DOIUrl":"https://doi.org/10.24018/ejbio.2021.2.5.265","url":null,"abstract":"Introduction: Malaria parasite is a protozoan disease that is transmitted by female anopheles mosquito which infects humans regardless of age, sex and status. It has a worldwide distribution and often prevalent in the developing countries and areas with poor environmental hygiene this study focuses on the incidence of plasmodiasis in relation to haematological parameters among children in the Internally Displaced Persons (IDPs) camp. \u0000Methods: blood samples were collected via venipuncture for thick blood film and was stained with giemsa diluted 1:10 and rapid diagnostic techniques (RDT), while the hematological parameter were analyzed by auto analyzer machine. \u0000Results: a total of two hundred and one (201) samples were obtained from two different camps in the study area. 87 were obtained from Stadium IDPs camp and 114 from Bakassi camp. From the Stadium IDPs camp 25(12.4%) were malaria positive and 62(30.8%) were negative. Similarly, from Bakassi IDPs camp, 58(28.9%) were positive and 56(27.9%) were negative. \u0000Conclusions: Males were shown to have eosinophilia compared to the females, due to an increase in the eosinophil count in them which can be used to predict the intensity of malaria infection, and a decrease in the eosinophil in females. There was a partial negative correlation due to a decrease in the monocyte and lymphocyte with increasing parasite density count.","PeriodicalId":72969,"journal":{"name":"European journal of biology and biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45976621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-10DOI: 10.24018/ejbio.2021.2.5.275
Rajkumar Bhosale, Sasidharan Sakkan, S. Padmanabhan
In this review, we highlight the complications of COVID-19 affected patients due to microbial infections, which increase the severity of the disease. Nearly 50% of COVID-19 affected patients among non-survivors were either co-infected with bacterial, fungal, or viral pathogens. During ongoing COVID-19 pandemic, it has been a challenge for developing and under developing countries to identify co-infections in patients due to limited healthcare facilities and high cost for the diagnostic tests. Since several microbial co-infections are associated with COVID-19, there is need to diagnose such co-infections in early stage so that required control measures would be taken to avoid the further health risks. People with severe COVID-19, COVID-19 patients in intensive care units (ICU), are susceptible to bacterial and fungal infections. Bacterial pathogens, representing less than 14% of patients with reported infections include Mycoplasma pneumoniae, Haemophilus influenzae and Pseudomonas aeruginosa while fungal sps include Aspergillus, Candida auris, black fungus etc that invade the brain or cause patients to lose vision. The unregulated and inappropriate use of antibiotics, antimicrobial drugs and alcohol based hand sanitizers may enhance the evolution of AMR phenotypes among infectious pathogens.
{"title":"Microbial Co-infections in Covid Patients: A Mini Review","authors":"Rajkumar Bhosale, Sasidharan Sakkan, S. Padmanabhan","doi":"10.24018/ejbio.2021.2.5.275","DOIUrl":"https://doi.org/10.24018/ejbio.2021.2.5.275","url":null,"abstract":"In this review, we highlight the complications of COVID-19 affected patients due to microbial infections, which increase the severity of the disease. Nearly 50% of COVID-19 affected patients among non-survivors were either co-infected with bacterial, fungal, or viral pathogens. During ongoing COVID-19 pandemic, it has been a challenge for developing and under developing countries to identify co-infections in patients due to limited healthcare facilities and high cost for the diagnostic tests. Since several microbial co-infections are associated with COVID-19, there is need to diagnose such co-infections in early stage so that required control measures would be taken to avoid the further health risks. People with severe COVID-19, COVID-19 patients in intensive care units (ICU), are susceptible to bacterial and fungal infections. Bacterial pathogens, representing less than 14% of patients with reported infections include Mycoplasma pneumoniae, Haemophilus influenzae and Pseudomonas aeruginosa while fungal sps include Aspergillus, Candida auris, black fungus etc that invade the brain or cause patients to lose vision. The unregulated and inappropriate use of antibiotics, antimicrobial drugs and alcohol based hand sanitizers may enhance the evolution of AMR phenotypes among infectious pathogens.","PeriodicalId":72969,"journal":{"name":"European journal of biology and biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47336394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-30DOI: 10.24018/ejbio.2021.2.5.249
J. R. Latournerié-Cervera, A. R. Estrada-Ortega, A. K. Escobar-Álvarez, A. J. Quino-Trejo, R. G. Barrera-Pastrana, Y. Nacif-Osorio
The protected natural area (ANP), Ejidos de Xochimilco and San Gregorio Atlapulco (SGA), is a priority area of ecological conservation in Mexico City (CDMX), that still have remnants of the ancient lake system of the Valley of Mexico watershed that have been modified for decades due to the impact of human activities and that threaten their continuity, despite been declared as a RAMSAR site and historical and cultural heritage of CDMX. Given the above, the present study aimed to compare the spatial-temporal variability of physical-chemical factors and phytoplankton as indicators of anthropogenic impact to diagnose the current state of this locality and propose possible alternatives for sustainable management in the study area. The research design considered comparing the area of channels and lagoons of the SGA wetland in two contrasting climatic seasons: dry and rainy. Eleven sampling stations were delimited: three in the channels area and eight in the lagoons, during the months of March and July. Nine physical-chemical variables were measured: temperature, pH, total solids, conductivity, dissolved oxygen, as well as the concentrations of ammonium, nitrites, nitrates, and soluble phosphorus in surface and bottom samples from each sampling station. The statistical design took into account a factorial design of fixed effects (epochs - locations and strata), with Tukey's "post hoc" test to delimit subsets and various multivariate analysis techniques. Phytoplankton samples were taken in both localities, which were identified down to the genus level, evaluating the relative frequency of the various taxa, as well as their association through the saprobity and diversity indexes. The water quality variables indicated that both the area of channels and the lagoons of the wetland are sites with hypereutrophic characteristics, but the wetland due to its semi-isolation is an area that presents on average levels of nitrates 2.4 times lower than in the channel zone, nitrite concentrations are almost imperceptible, and ammonia is within tolerable limits for the existing biota. It also presents space-time homogeneity in its physical-chemical dynamics, in contrast to the area of channels. The composition of species indicating saprobity also differed between both sites, denoting a lesser impact on the wetland area, so it is suggested to use this site for reintroduction activities of species removed from the channel area and preserve the remaining biota in the lake system.
自然保护区（ANP），Ejidos de Xochimilco和San Gregorio Atlapulco（SGA），是墨西哥城（CDMX）生态保护的优先区域，这些区域仍然保留着墨西哥河谷流域古老湖泊系统的残余，由于人类活动的影响，这些系统已经修改了几十年，并威胁到其连续性，尽管已被宣布为RAMSAR遗址和CDMX的历史文化遗产。鉴于上述情况，本研究旨在比较物理化学因素和浮游植物作为人为影响指标的时空变异性，以诊断该地区的现状，并提出研究区域可持续管理的可能替代方案。研究设计考虑了在干旱和雨季这两个截然不同的气候季节比较SGA湿地的河道和泻湖面积。在3月和7月期间，划定了11个采样站：3个在海峡地区，8个在泻湖。测量了九个物理化学变量：温度、pH、总固体、电导率、溶解氧，以及每个采样站表面和底部样品中铵、亚硝酸盐、硝酸盐和可溶性磷的浓度。统计设计考虑了固定效应（时期-位置和地层）的析因设计，使用Tukey的“事后”检验来划分子集和各种多元分析技术。在这两个地方都采集了浮游植物样本，并对其进行了属级鉴定，评估了各种分类群的相对频率，以及它们通过腐性和多样性指数的关联性。水质变量表明，湿地的河道和泻湖区域都具有超富营养特征，但由于半隔离，湿地的硝酸盐平均水平是河道区域的2.4倍，亚硝酸盐浓度几乎难以察觉，氨在现有生物群的可容忍范围内。与通道的面积相比，它在物理化学动力学方面也表现出时空同质性。两个地点的物种组成也不同，表明对湿地区域的影响较小，因此建议利用该地点对从河道区域移除的物种进行重新引入活动，并保护湖泊系统中剩余的生物群。
{"title":"Physico-chemical Factors an Phytoplankton as Indicators of the Water Quality in Two Periurbans Lakes of a Natural Protected Area in Mexico City","authors":"J. R. Latournerié-Cervera, A. R. Estrada-Ortega, A. K. Escobar-Álvarez, A. J. Quino-Trejo, R. G. Barrera-Pastrana, Y. Nacif-Osorio","doi":"10.24018/ejbio.2021.2.5.249","DOIUrl":"https://doi.org/10.24018/ejbio.2021.2.5.249","url":null,"abstract":"The protected natural area (ANP), Ejidos de Xochimilco and San Gregorio Atlapulco (SGA), is a priority area of ecological conservation in Mexico City (CDMX), that still have remnants of the ancient lake system of the Valley of Mexico watershed that have been modified for decades due to the impact of human activities and that threaten their continuity, despite been declared as a RAMSAR site and historical and cultural heritage of CDMX. Given the above, the present study aimed to compare the spatial-temporal variability of physical-chemical factors and phytoplankton as indicators of anthropogenic impact to diagnose the current state of this locality and propose possible alternatives for sustainable management in the study area. The research design considered comparing the area of channels and lagoons of the SGA wetland in two contrasting climatic seasons: dry and rainy. Eleven sampling stations were delimited: three in the channels area and eight in the lagoons, during the months of March and July. Nine physical-chemical variables were measured: temperature, pH, total solids, conductivity, dissolved oxygen, as well as the concentrations of ammonium, nitrites, nitrates, and soluble phosphorus in surface and bottom samples from each sampling station. The statistical design took into account a factorial design of fixed effects (epochs - locations and strata), with Tukey's \"post hoc\" test to delimit subsets and various multivariate analysis techniques. Phytoplankton samples were taken in both localities, which were identified down to the genus level, evaluating the relative frequency of the various taxa, as well as their association through the saprobity and diversity indexes. The water quality variables indicated that both the area of channels and the lagoons of the wetland are sites with hypereutrophic characteristics, but the wetland due to its semi-isolation is an area that presents on average levels of nitrates 2.4 times lower than in the channel zone, nitrite concentrations are almost imperceptible, and ammonia is within tolerable limits for the existing biota. It also presents space-time homogeneity in its physical-chemical dynamics, in contrast to the area of channels. The composition of species indicating saprobity also differed between both sites, denoting a lesser impact on the wetland area, so it is suggested to use this site for reintroduction activities of species removed from the channel area and preserve the remaining biota in the lake system.","PeriodicalId":72969,"journal":{"name":"European journal of biology and biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47724167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-24DOI: 10.24018/ejbio.2021.2.5.252
A. Kouakou, G. Doué, Rose-Monde Megnanou, A. E. S. Djoman
Shea nuts shells and press cake are shea butter processing byproducts. Their content in phenolic bioactive compounds and their antioxidant capacity were studied. The total phenolic and flavonoid contents ranged between 16.962-21.395 mg GAE/100g DM and 0.243-1.83 mg QE/100g DM, for press-cake and shells, respectively. Antioxidant capacity values assessed by EC50 using DPPH, ABTS and FRAP methods were in the range of 0.398-0.235 mg/mL, 0.623-0.465 mg/mL and 0.137-0.154 mg/mL for shea press-cake and shells, respectively. Shea shells recorded the highest total phenolic and flavonoid compounds positively correlated with DPPH and ABTS radical scavenging. Catechins, arbutin and gallic acid were the most abundant phenolic compound identified by GC-MS chromatography with 10.4; 2.1 and 0.8 g/100 g in shea shells and 2.2; 2.6 and 2.6 g/100 g in press-cake. Based on this phenolic composition shea nuts shells and press cake could be of great importance in cosmetic and pharmaceutical industries, and also contribute to preventing metabolic and cardiovascular diseases.
{"title":"Shea Shells and Press-Cake as New Sources of Bioactive Phenolic Compounds: GC MS Profile and in vitro Antioxidant Activity","authors":"A. Kouakou, G. Doué, Rose-Monde Megnanou, A. E. S. Djoman","doi":"10.24018/ejbio.2021.2.5.252","DOIUrl":"https://doi.org/10.24018/ejbio.2021.2.5.252","url":null,"abstract":"Shea nuts shells and press cake are shea butter processing byproducts. Their content in phenolic bioactive compounds and their antioxidant capacity were studied. The total phenolic and flavonoid contents ranged between 16.962-21.395 mg GAE/100g DM and 0.243-1.83 mg QE/100g DM, for press-cake and shells, respectively. Antioxidant capacity values assessed by EC50 using DPPH, ABTS and FRAP methods were in the range of 0.398-0.235 mg/mL, 0.623-0.465 mg/mL and 0.137-0.154 mg/mL for shea press-cake and shells, respectively. Shea shells recorded the highest total phenolic and flavonoid compounds positively correlated with DPPH and ABTS radical scavenging. Catechins, arbutin and gallic acid were the most abundant phenolic compound identified by GC-MS chromatography with 10.4; 2.1 and 0.8 g/100 g in shea shells and 2.2; 2.6 and 2.6 g/100 g in press-cake. Based on this phenolic composition shea nuts shells and press cake could be of great importance in cosmetic and pharmaceutical industries, and also contribute to preventing metabolic and cardiovascular diseases.","PeriodicalId":72969,"journal":{"name":"European journal of biology and biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47163771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-21DOI: 10.24018/ejbio.2021.2.5.222
I. Ouattara, Claver Zea Bi Ue, S. Berté, Bakagnan Kamagate
This study was conducted to evaluate the feeding levels on survival, growth performance and feed utilization in the Brazil strain of Nile tilapia Oreochromis niloticus. Four (4) batches of larvae with mean initial weight 0.012 ± 0.005 g and mean initial total length 9 ± 0.003 mm were formed in triplicate, three per feeding rate. The rationing rates according to fry biomass applied in this trial were: batch 1: 50, 40, 30 and 25%, batch 2: 40, 30, 25 and 20%, batch 3: 30, 25, 20 and 18% and batch 4: 25, 20, 18 and 15% of biomass. The different feeding rates were tested during the 1st, 2nd, 3rd and 4th week of larval rearing respectively. The fish were all fed a commercial feed (48% protein) distributed manually at a frequency of 5 meals per day. After 28 days of rearing, the results show that apart from the survival rate, the other parameters such as final average weight, daily growth and food consumption index were affected by the different rationing rates tested. The larvae of batch 2 rationed at 40, 30, 25 and 20 % of their biomass during the 1st, 2nd, 3rd and 4th week of rearing respectively recorded significantly higher growth parameters (MWF: 1.092±0.027 g and DG: 0.038±0.000 g) and a more interesting food converion ratio (0.88 ) than the other batches of fry. From this study, it is concluded that an optimal feeding rate of 40, 30, 25 and 20% of the larval biomass during the 1st, 2nd, 3rd and 4th week of rearing, respectively, should be applied in order to avoid either wastage or underfeeding of the subjects during hormone treatment with 17 α-methyltestosterone.
{"title":"Effect of Feeding Rate on Survival, Zootechnical Performance of Tilapia Oreochromis niloticus (Linnaeus, 1758) Brazil Strain Larvae Fed on 17-α-methyltestosterone Treated Feed","authors":"I. Ouattara, Claver Zea Bi Ue, S. Berté, Bakagnan Kamagate","doi":"10.24018/ejbio.2021.2.5.222","DOIUrl":"https://doi.org/10.24018/ejbio.2021.2.5.222","url":null,"abstract":"This study was conducted to evaluate the feeding levels on survival, growth performance and feed utilization in the Brazil strain of Nile tilapia Oreochromis niloticus. Four (4) batches of larvae with mean initial weight 0.012 ± 0.005 g and mean initial total length 9 ± 0.003 mm were formed in triplicate, three per feeding rate. The rationing rates according to fry biomass applied in this trial were: batch 1: 50, 40, 30 and 25%, batch 2: 40, 30, 25 and 20%, batch 3: 30, 25, 20 and 18% and batch 4: 25, 20, 18 and 15% of biomass. The different feeding rates were tested during the 1st, 2nd, 3rd and 4th week of larval rearing respectively. The fish were all fed a commercial feed (48% protein) distributed manually at a frequency of 5 meals per day. After 28 days of rearing, the results show that apart from the survival rate, the other parameters such as final average weight, daily growth and food consumption index were affected by the different rationing rates tested. The larvae of batch 2 rationed at 40, 30, 25 and 20 % of their biomass during the 1st, 2nd, 3rd and 4th week of rearing respectively recorded significantly higher growth parameters (MWF: 1.092±0.027 g and DG: 0.038±0.000 g) and a more interesting food converion ratio (0.88 ) than the other batches of fry. From this study, it is concluded that an optimal feeding rate of 40, 30, 25 and 20% of the larval biomass during the 1st, 2nd, 3rd and 4th week of rearing, respectively, should be applied in order to avoid either wastage or underfeeding of the subjects during hormone treatment with 17 α-methyltestosterone.","PeriodicalId":72969,"journal":{"name":"European journal of biology and biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48041076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-12DOI: 10.24018/ejbio.2021.2.5.258
A. Goc, V. Ivanov, S. Ivanova, M. Chatterjee, M. Rath, A. Niedzwiecki
SARS-CoV-2 pandemic remains a challenge to human health and economy worldwide. Previously we have shown that a combination of active plant-derived compounds and plant extracts can dose-dependently inhibit binding of RBD-spike protein SARS-CoV-2 to the ACE2 receptor and its expression on human alveolar epithelial cells. Here we use eGFP-luciferase-SARS-CoV-2 spike protein pseudo-virions and SARS-CoV-2-RdRp, to show if the antiviral effectiveness of this combination of plant-derived compounds and plant extracts expands to other important key mechanisms of SARS-CoV-2 infection. Or results revealed that this combination of five plant-derived compounds inhibited the attachment of the SARS-CoV-2 pseudo-typed particles with lung hACE2/A549 cells. In addition, it down-regulated the activity of key enzymes known to be crucial for the entry of the SARS-CoV-2 virus, such as TMPRSS2, furin and cathepsin L, but not their expression at protein level. This combination did not affect ACE2 binding to and ACE2 enzymatic activity, but modestly decrease cellular expression of neuropilin-1 molecule and significantly inhibited activity of viral RdRp. This study demonstrates inhibitory effects of this combination on key cellular mechanisms of SARS-CoV-2 infection. The findings further support the use of plant-derived compounds as effective health measures against SARS-CoV-2-caused infection.
{"title":"Simultaneous Inhibition of SARS-CoV-2 Infectivity by a Specific Combination of Plant-derived Compounds","authors":"A. Goc, V. Ivanov, S. Ivanova, M. Chatterjee, M. Rath, A. Niedzwiecki","doi":"10.24018/ejbio.2021.2.5.258","DOIUrl":"https://doi.org/10.24018/ejbio.2021.2.5.258","url":null,"abstract":"SARS-CoV-2 pandemic remains a challenge to human health and economy worldwide. Previously we have shown that a combination of active plant-derived compounds and plant extracts can dose-dependently inhibit binding of RBD-spike protein SARS-CoV-2 to the ACE2 receptor and its expression on human alveolar epithelial cells. Here we use eGFP-luciferase-SARS-CoV-2 spike protein pseudo-virions and SARS-CoV-2-RdRp, to show if the antiviral effectiveness of this combination of plant-derived compounds and plant extracts expands to other important key mechanisms of SARS-CoV-2 infection. Or results revealed that this combination of five plant-derived compounds inhibited the attachment of the SARS-CoV-2 pseudo-typed particles with lung hACE2/A549 cells. In addition, it down-regulated the activity of key enzymes known to be crucial for the entry of the SARS-CoV-2 virus, such as TMPRSS2, furin and cathepsin L, but not their expression at protein level. This combination did not affect ACE2 binding to and ACE2 enzymatic activity, but modestly decrease cellular expression of neuropilin-1 molecule and significantly inhibited activity of viral RdRp. This study demonstrates inhibitory effects of this combination on key cellular mechanisms of SARS-CoV-2 infection. The findings further support the use of plant-derived compounds as effective health measures against SARS-CoV-2-caused infection.","PeriodicalId":72969,"journal":{"name":"European journal of biology and biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47666448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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