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Study on Preparation of New Purified Vero Cell Rabies Vaccine 新型纯化Vero细胞狂犬病疫苗的制备研究
Pub Date : 2019-08-20 DOI: 10.33140/jcei.04.04.04
Rabies is a 100% vaccine-preventable disease. A cheap, rabies vaccine for humans that could be used in mass vaccinationcampaigns would be a valuable weapon against rabies. Despite the existence of safe and effective vaccines, rabies diseasestill causes an estimated more human deaths a year in the endemic areas in Asia and Africa. Rabies virus is single strandednegative sense RNA from genus lyssavirus and _ family rhabdoviridae.
狂犬病是一种100%可以通过疫苗预防的疾病。一种廉价的、可用于大规模疫苗接种运动的人类狂犬病疫苗将成为对抗狂犬病的宝贵武器。尽管存在安全有效的疫苗,但在亚洲和非洲的流行地区,狂犬病每年造成的死亡人数估计仍然更多。狂犬病毒是来自狂犬病毒属和横纹肌病毒科的单链负链RNA。
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引用次数: 0
Biostatistical Analysis on Chronic Disease Risk 慢性疾病风险的生物统计学分析
Pub Date : 2019-08-08 DOI: 10.33140/jcei.04.04.02
B. Zhao, X. Jiang, J. Cao, K. Huang
Introduction: Approximately one-third of all college students aged 18-29 were classified as overweight or obese, increasing theirrisk for chronic disease. Nutritional knowledge is an important first step to developing strategist to improve the food choicesof college students and promote healthy lifestyle behaviors. Therefore, the purpose of this study was to determine the currentnutritional knowledge of students enrolled in courses within the Human Performance and Leisure Studies (HPLS) Departmentat Hubei University of Technology (HBUT).Methods: Paper based surveys were used to administer the 56-question Nutritional Knowledge Questionnaire (NKQ) for studentsenrolled in HPLS courses in spring 2017.Results: Response rate was 65.6% (n=236). Gender distribution was equal among the surveyed students (45.3% male vs. 53.8%females). The majority reported majoring in sports science and fitness management (SSFM) (76.7%), were between 18-24 yearsold (86.9%) with a self-identified ethnic origin of Chinese Xinjiang person (89.8%). The average nutritional knowledge scorewas 49.2 +/- 9.8 or 44.8%. Knowledge of diet-disease relationship was higher in females (5.4 +/- 1.9) compared to males (4.8+/- 2.3), p = 0.03.Conclusion: In this study, we found a need to improve nutritional knowledge in college students enrolled in select HPLS coursesin the spring of 2017 at HBUT. The findings highlight a gap in nutrition education and the opportunity to develop courses orprograms aimed at healthful eating for students majoring in SSFM or attending HBUT.
在18-29岁的大学生中,大约有三分之一的人被归类为超重或肥胖,这增加了他们患慢性疾病的风险。营养知识是制定策略改善大学生食物选择和促进健康生活方式的重要第一步。因此,本研究的目的是确定湖北工业大学(HBUT)人体表现与休闲研究(HPLS)专业在校生的营养知识现状。方法:采用纸质问卷调查的方法,对2017年春季HPLS课程在校生进行56题营养知识问卷(NKQ)调查。结果:有效率为65.6% (n=236)。受访学生性别分布基本一致(45.3%男生vs 53.8%女生)。以运动科学与健身管理(SSFM)专业为主(76.7%),年龄在18-24岁之间(86.9%),自认民族为中国新疆人(89.8%)。平均营养知识得分为49.2 +/- 9.8或44.8%。女性对饮食与疾病关系的认知(5.4 +/- 1.9)高于男性(4.8+/- 2.3),p = 0.03。结论:在本研究中,我们发现HBUT 2017年春季选读HPLS课程的大学生需要提高营养知识。研究结果强调了营养教育方面的差距,以及为SSFM专业或HBUT专业的学生开发健康饮食课程或项目的机会。
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引用次数: 0
Outcome of External Ventricular Drainage in Spontaneous IntracerebralHaemorrhage with Ventricular Extension in different GCS Score 脑室外引流治疗自发性脑出血伴脑室扩张患者不同GCS评分的疗效
Pub Date : 2019-06-07 DOI: 10.33140/jcei.04.03.05
Background: Intracerebral Haemorrhage (ICH) is a medical emergency of the highest degree with frequent early neurologicaldeterioration or death. External ventricular drainage (EVD) is the procedure of choice for the treatment of spontaneousintracerebral haemorrhage with ventricular extension or blood within the ventricles, acute hydrocephalus and increasedintracranial pressure in patients of intracerebral haemorrhage and subarachnoid haemorrhage (SAH) with hydrocephalus andits sequelae.Objective: The aim and objective of this study was to predict the outcome of pre operative GCS following external ventriculardrainage in spontaneous intracerebral haemorrhage with ventricular extension.Method: In this was prospective observational studies, a total number of 60 cases were taken purposively for a period of July,2015- March, 2017 diagnosed by CT scan of brain at Department of Neurosurgery, Dhaka Medical College Hospital. All thepatients, fulfilling the inclusion and exclusion criteria, were enrolled for the study. For assessing outcome of EVD in post operativepatients and evaluating the efficacy of EVD surgery in follow ups. Glasgow Coma Scale and Glasgow Outcome Scale scoringmethod for patient assessment were used for outcome of EVD surgery.Result: A total of 60 patients were included in this study, age range was 45 to 86 years. Majority patients, 24 (40.0%) were from61-70 years of age. The mean age was found 62.0±20. It was observed that 24 (40.00%) patients had GCS 5. GCS 6 was observedin 14(23.33%) patients. GCS 5 and 6 were not found post operatively in any cases. GCS 7 was observed in 14 (23.33%) cases.Whereas, GCS 8, 9, 10 were found in 11 (18.33%), 13 (21.66%), 10(16.66%) cases respectively and 4 cases were died on firstpost operative day. In most cases GCS level rose to 2 points. GOS at 7th POD died total 12 (20.00%) cases. It was observedthat 48 (80.00%) patients were alive Moderate disability existed in 12(25.00%) cases. Again, severe disability and persistentvegetative cases observed in 14(29.16%), 9(18.75%) cases. Glasgow Outcome Scale at 3 months follow up of my study patients,it was observed that total died patients 16 (26.66%).Conclusion: According to my study, majority of the study patients survived following EVD in spontaneous ICH with ventricularextension but most cases was unfavorable outcome which was statistically
背景:脑出血(ICH)是一种严重程度最高的医学急症,常伴有早期神经系统恶化或死亡。脑室外引流术(EVD)是脑出血和蛛网膜下腔出血(SAH)合并脑积水及其后遗症的自发性脑出血或脑室内出血、急性脑积水和颅内压增高的首选治疗方法。目的:本研究的目的是预测自发性脑出血伴脑室扩张患者行脑室外引流术后术前GCS的预后。方法:选取2015年7月- 2017年3月在达卡医学院附属医院神经外科行颅脑CT扫描诊断的60例患者为前瞻性观察性研究。所有符合纳入和排除标准的患者均被纳入研究。目的:评价EVD术后患者的预后,评价EVD手术的随访效果。EVD手术结果采用格拉斯哥昏迷评分法和格拉斯哥预后评分法进行患者评估。结果:本研究共纳入60例患者,年龄45 ~ 86岁。大多数患者,24例(40.0%)年龄在61-70岁之间。平均年龄62.0±20岁。GCS 5级患者24例(40.00%)。14例(23.33%)患者GCS为6。术后未发现GCS 5、6。GCS 7级14例(23.33%)。GCS 8、9、10分别为11例(18.33%)、13例(21.66%)、10例(16.66%),术后第1天死亡4例。在大多数情况下,GCS水平上升到2分。第7期GOS死亡12例(20.00%)。存活48例(80.00%),存在中度残疾12例(25.00%)。重度残疾和持续植物人分别为14例(29.16%)和9例(18.75%)。格拉斯哥结局量表对我的研究患者随访3个月时,观察到总死亡患者16例(26.66%)。结论:根据我的研究,EVD后自发性脑出血伴脑室扩张的患者绝大多数存活,但多数为不良结局,有统计学意义
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引用次数: 0
The Effects of Intraoperative Hypothermia Review of the Molecular Mechanisms ofAction in Therapeutic Hypothermia 术中低温的影响治疗性低温的分子机制综述
Pub Date : 2019-06-03 DOI: 10.33140/jcri.04.03.03
During surgery the patient may lose heat during and after surgerythrough the contribution of several factors: ambient temperature,cold fluid infusion, the position on the operating table, surgicalskin preparation methods, type of surgery, conventional surgeryor laparoscopy, and the loss increase of the heat by opening theserous cavities, thoracic or abdominal [1]. They add other factors,depending on patients: the elderly are more prone to heat loss,sex; women lose less heat, the existence of associated diseases, asperipheral vascular diseases, endocrine diseases, cachexia, physicalconstitution or presence of pregnancy. Temperature of the patient’sbody lowers in relation to prolonged patient stay in a cool room ofresuscitation. The heat loss of the skin tissue in the operating roomis important and is expressed at approximately 100 W [2]. Moreimportant than the relationship between temperature of the operatingroom and patient’s skin, the microclimate, which is establishedbetween operators fields and patient. Another important factor isbody surface area exposed having significant area reported at weight.Hypothermia is aggravated by cold fluid administration, abdominalor thoracic wounds. The use of cold solutions in urologic surgeryexposes the central temperature drop, which is more marked ifintervention is performed under epidural anesthesia [3]. In epiduralanesthesia, hypothermia is due to redistribution of heat betweenthe center and periphery, the thighs being established to intensevasodilatation and heat loss [4, 5]. All measures taken to preventheat loss are important for prevention of coagulation disorders.Hypothermia reduces oxygen release in half, reducing the liver’sability to metabolize citrate and lactic acid and cause arrhythmia.The existence of hypothermia in surgical patient reflects failurethermoregulatory mechanisms [1-3].
在手术过程中,患者可能会在手术中和手术后由于以下几个因素而失去热量:环境温度、冷液输注、手术台上的位置、手术皮肤准备方法、手术类型、常规手术或腹腔镜手术,以及通过打开硬膜腔、胸腔或腹腔而增加的热量损失。他们还根据病人的情况添加了其他因素:老年人更容易失热、性;女性失热少,存在相关疾病,如外周血管疾病、内分泌疾病、恶病质、体质或存在妊娠。病人在凉爽的复苏室内待的时间越长,病人的体温就越低。手术室中皮肤组织的热损失很重要,约为100瓦/桶。比手术室温度与患者皮肤之间的关系更重要的是,操作者现场与患者之间建立的小气候。另一个重要的因素是暴露的体表面积有显著的体重报告。低温症会因冷液注射、腹部胸部伤口而加重。在泌尿外科手术中使用冷溶液会暴露出中心温度下降,在硬膜外麻醉下进行干预时,这种下降更为明显。在硬膜外麻醉中,低温是由于热量在中心和外周之间的重新分配,大腿被建立以加强血管扩张和热量损失[4,5]。所有预防治疗损失的措施对预防凝血功能障碍都很重要。低温使氧气释放减少一半,降低肝脏代谢柠檬酸盐和乳酸的能力,导致心律失常。手术患者体温过低反映了体温调节机制的失效[1-3]。
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引用次数: 0
Multimodal Approach with CAR-T and CAR-NK Cells for Synergistic Action toTreat Cancer CAR-T和CAR-NK细胞协同治疗癌症的多模式方法
Pub Date : 2019-04-09 DOI: 10.33140/jcei.04.03.02
Recent years have seen a craze about the T-cell immunotherapy for B-cell malignancies, and there is growing interest in NK-CAR therapy. In this paper, I discuss the similarities and differences between the CARs in the NK and T-Cells. Further, explore the possible theoretical concept of a multimodal approach towards treating cancer. Whether the two genetically modified cells could provide an added advantage acting synergistically to complement each other’s therapeutic qualities or counteract each other’s downfall. This short communication is an attempt to pique interest to understand the mechanisms of NK cell and T cell biological interaction in the presence of a therapeutic weapon “CAR” to target tumor.
近年来,人们对b细胞恶性肿瘤的t细胞免疫疗法产生了浓厚的兴趣,nk - car疗法也引起了人们的兴趣。在本文中,我讨论了NK细胞和t细胞中的car的异同。进一步探讨多模式治疗癌症的可能理论概念。这两种基因修饰的细胞是否能提供额外的优势,协同作用,以补充对方的治疗品质或抵消对方的垮台。这种简短的交流是为了激起人们对理解NK细胞和T细胞在靶向肿瘤的治疗武器“CAR”存在下生物相互作用机制的兴趣。
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引用次数: 0
The Sea Star Igkappa Gene and The Ophuirid Igkappa Gene Vaccinations to HRP(Horse-radish Peroxydase) 海星Igkappa基因和Ophuirid Igkappa基因接种马萝卜过氧化物酶(HRP)的研究
Pub Date : 2019-04-01 DOI: 10.33140/jcei.04.03.01
M. Leclerc
The main point of the sea star immunology, of the ophuirid immunology remain the discovery of the INVERTEBRATE PRIMITIVEANTIBODY (IPA), the IGKAPPA GENES, with Ig sites which imply the COMPLEMENT SYSTEM, to be initiated: 9 componentgenes from C1 to C9 have been updated these last years, in sea star genome, in ophuirid one. These primitive antibodies wereobtained after immunizations to the enzyme HRP (Horse-radish peroxydase). It is the first time we observe vaccinations ininvertebrates. We have discovered, for the first time also, in these same Invertebrate a FC receptor gene, a Fab gene whichcorroborate the presence of IPA. The transcriptomes are given. It is the first time, we can speak of Adaptative Immunity, inEchinoderms, in Invertebrates Since many years, even since a century long, the notion of Antibody was out of the speech ofimmunologists. To speak of that made you as an outlaw. It is time to look with genomic studies which confirm which assert nowevidence that 3 classes of Echinoderms out of 5 possess an Igkappa gene, a FC receptor gene. I recall these classent: the Asteridswith Asterias rubens, the Ophuirids with Ophiocomina nigra and the Crinoïds.
海星免疫学研究的重点是发现了无脊椎动物原始抗体(IPA), IGKAPPA基因,其Ig位点暗示着补体系统的启动:近年来,海星基因组中从C1到C9的9个组成基因已被更新。这些原始抗体是在免疫马萝卜过氧化物酶(HRP)后获得的。这是我们第一次观察到无脊椎动物接种疫苗。我们也首次在这些无脊椎动物中发现了FC受体基因和Fab基因,证实了IPA的存在。转录组是已知的。这是第一次,我们可以在棘皮动物,无脊椎动物中,谈论适应性免疫多年来,甚至一个世纪以来,抗体的概念从免疫学家的语言中消失了。说到这,你就像个亡命之徒。现在是时候进行基因组研究了,这些研究证实了5类棘皮动物中有3类拥有Igkappa基因,这是一种FC受体基因。我记得这些分类:asterids与Asterias rubens, ophuiids与Ophiocomina nigra和Crinoïds。
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引用次数: 0
A Unique Vaccine with Multiple Applications 一种具有多种用途的独特疫苗
Pub Date : 2019-03-21 DOI: 10.33140/jcei.04.02.13
G. Talwar, J. C. Gupta, A. Krishnan, A. Mustafa, S. Zaheer, HK Kar, R. Mukherjee, R. Rani, S. Bhaskar, P. Reddi, V. Saini, Seyed Hasnain, A. Tyagi, Anil K Tyagi, Somesh Gupta, D. Nandi, Shilpi, Purswani
The immune system consists of molecules and cells (and theirproducts) to keep the Body free of afflictions. Clinical problems ariseas and when there is failure or inadequate immunological response.How can the immune system be fortified against an undesirableinfection? This is done by making of a vaccine against that infection.This article describes the development of a Vaccine against Leprosy.Most of us (>90%) are immune to this disease. The few, who becomevictims to leprosy, manifest pitiable symptoms. Interestingly thisvaccine has found multiple applications. It is a potent invigoratorof immune responses.
免疫系统由分子和细胞(及其产物)组成,使身体免受病痛。临床问题出现,当有失败或不充分的免疫反应。如何加强免疫系统抵御不良感染?这是通过制造针对这种感染的疫苗来实现的。这篇文章描述了麻风病疫苗的发展。我们大多数人(约90%)对这种疾病免疫。少数成为麻风病受害者的人,表现出可怜的症状。有趣的是,这种疫苗有多种用途。它是免疫反应的有力刺激剂。
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引用次数: 0
Extracorporeal Immunotherapy in Oncology 肿瘤体外免疫治疗
Pub Date : 2019-03-06 DOI: 10.33140/jcei.04.02.09
Tumors development is closely related to the immune system state and in immunosuppression tumors occur many times more often. The quality of the immune defense depends on how the recognition system functions for malignized tumor cells and its timely destruction. However, the immunosuppression state may be a result of the tumor process itself. The tumor itself generates soluble molecules that inhibit the killer activity of lymphocytes and macrophages, which allows tumor cells to survive in the body. Therefore, it is justified to perform apheresis therapy aimed at removal of such inhibitors, and targeted restoration of cytotoxic activity of leukocytes, which should contribute to the tumor cells apoptosis. This method of extracorporeal immunopharmacotherapy is indicated not only in far-advanced cases, but also after any radical operations, when metastases are not detected and even chemotherapy is not carried out.
肿瘤的发生与免疫系统的状态密切相关,在免疫抑制的情况下,肿瘤发生的频率要高很多倍。免疫防御的质量取决于识别系统对恶性肿瘤细胞的功能和对其的及时破坏。然而,免疫抑制状态可能是肿瘤过程本身的结果。肿瘤本身产生可溶性分子,抑制淋巴细胞和巨噬细胞的杀伤活性,使肿瘤细胞在体内存活。因此,有理由进行旨在去除这些抑制剂的单采治疗,并有针对性地恢复白细胞的细胞毒活性,这应该有助于肿瘤细胞的凋亡。这种体外免疫药物治疗方法不仅适用于晚期病例,也适用于任何根治性手术后,未发现转移甚至不进行化疗的情况。
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引用次数: 0
What is the Connection between Normal Body Temperature and Chicken Likeness? 正常体温和鸡的长相有什么关系?
Pub Date : 2019-03-04 DOI: 10.33140/jcei.04.02.12
The objective of current study was to co-relate normal body temperature with chicken likeness. Body temperature is the normal temperature of the body. Usual body temperature may change in different situations such as by age, person, time of the day and activity. Thermometer is the instrument used to find out the temperature of the body. Total of 150 students took part in the recent study and they were the students of Bahauddin Zakariya University, Multan Pakistan. We arranged the instrument and then measured their body temperature. At the end we can concluded that there is a strong relation between these two variables. The male and female individuals with high body temperature are chicken lovers.
本研究的目的是研究正常体温与鸡的相似度之间的关系。体温是人体的正常体温。正常体温会因年龄、人、一天中的时间和活动等不同情况而变化。温度计是用来测定身体温度的仪器。共有150名学生参加了最近的研究,他们是巴基斯坦木尔坦Bahauddin Zakariya大学的学生。我们布置好仪器,然后测量他们的体温。最后我们可以得出结论,这两个变量之间有很强的关系。体温高的雄性和雌性都爱吃鸡。
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引用次数: 0
Conjunction between the Ladyfinger Likeness and Human Normal Body Temperature in Fahrenheit 小指形与人体正常体温(华氏)的关联
Pub Date : 2019-03-02 DOI: 10.33140/jcei.04.02.05
The purpose of this study was to show the conjunction between the ladyfinger likeness and human normal body temperature. Normal body temperature in human is the temperature range in Homo sapiens. Normal body temperature in human ranged as 97.7 to 99.5 ºF. It is balanced by the process of thermoregulation. The optimum temperature in the human is 98.6 ºF which is 37.0 in degrees. Ladyfinger is the vegetable which is used to cook. Ladyfinger also improves the vision quality because in it vitamin A is present. During the project designing, we designed the project related to the linkage of likeness of ladyfinger with the normal body temperature. 131 companions were joined in this project studies to whom we asked about their normal body temperatures. These companions were the undergraduate students of University. We took the thermometer. Then we selected the site at which we wanted to measure the temperature. Students came to us one by one. Firstly one student came to us. We placed the thermometer in the mouth. The readings appeared on the thermometer after few seconds and we calculated the reading and noted down on the page. It is excluded that there is no conjunction between the ladyfinger likeness and human normal body temperature.
本研究的目的是为了显示小手指的外形与人体正常体温之间的联系。人的正常体温是智人的体温范围。人的正常体温范围为97.7至99.5华氏度。它是通过体温调节过程来平衡的。人体的最佳温度是华氏98.6度,即摄氏37.0度。青菜是用来做饭的蔬菜。Ladyfinger还能改善视力,因为其中含有维生素A。在项目设计中,我们设计了小手指外形与正常体温联动的相关项目。131名同伴参加了这个项目研究,我们询问了他们的正常体温。这些同伴是大学的本科生。我们拿了温度计。然后我们选择我们想要测量温度的地点。学生们一个接一个地来到我们这里。首先,一个学生来找我们。我们把体温计放进嘴里。几秒钟后,温度计上的读数出现了,我们计算了一下读数,并把它记在纸上。排除了小指与人体正常体温之间没有联系的可能性。
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引用次数: 0
期刊
Journal of clinical & experimental immunology
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