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In-vitro antimicrobial activities of Euphorbia hirta against some clinical isolates 大戟对部分临床分离株的体外抗菌活性研究
Pub Date : 2012-04-01 DOI: 10.5251/ABJNA.2012.3.4.169.174
Kareem Titilope, E. Rashidat
This study was carried out to investigate the antibacterial activities of both ethanol and water extracts of Euphorbia hirta against some pathogens: Escherichia coli, Haemophilus influenzae, Klebsiella pneumoniae, Proteus vulgaris, Pseudomonas aeruginosa, Staphylococcus aureus, Salmonella typhi and Shigella dysenteriae. The agar diffusion method was used to determine the antibacterial activities of the extracts against the pathogens while the minimum inhibitory concentration (MIC) was determined by graphical method. Antibacterial sensitivity test indicated that the extracts of E. hirta inhibited the growth of all the organisms to varying degree. Significantly little or no zone of inhibition was observed when E. hirta extracts were tested on H. influenzae. Dry leaf extracts of E. hirta produced the highest zones of inhibition on Salmonella typhi. The dry extracts produced MIC values ranging between 2.78mg/ml to 41.92mg/ml for all the organisms while that of the fresh ethanol extracts ranged from 0.27mg/ml to 20.13mg/ml.
研究了大戟乙醇提取物和水提取物对大肠杆菌、流感嗜血杆菌、肺炎克雷伯菌、普通变形杆菌、铜绿假单胞菌、金黄色葡萄球菌、伤寒沙门菌和痢疾志贺氏菌的抑菌活性。用琼脂扩散法测定提取物对病原菌的抑菌活性,用图解法测定提取物的最低抑菌浓度(MIC)。抑菌敏感性试验表明,赤毛杆菌提取物对所有细菌的生长均有不同程度的抑制作用。当hirta提取物对流感嗜血杆菌进行测试时,明显很少或没有观察到抑制区。干叶提取物对伤寒沙门氏菌的抑制作用最强。干燥提取物的MIC值在2.78 ~ 41.92mg/ml之间,新鲜乙醇提取物的MIC值在0.27 ~ 20.13mg/ml之间。
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引用次数: 22
Performance of wheat yield under different fertilizer types, application and doses at Northern Sudan State 北苏丹州不同肥料类型、施用和剂量下的小麦产量表现
Pub Date : 2012-03-01 DOI: 10.5251/ABJNA.2012.3.3.125.130
S. Mahgoub, Y. Ibrahim
The experiment was conducted during the successive seasons of 2008/2009 and 2009/2010 at the Demonstration Farm of the Faculty of Agricultural Sciences, University of Dongola, Northern State, Sudan. The aim of this experiment was to study the effect of fertilizer doses, types and application methods on the grain yield of wheat (Triticum aestivum L.). Experimental method is a split-split plot trial with four replications. The main plot was composed of tow methods of fertilizer application (broadcasting and localized placement) in randomized complete block design. Subplots consisted of tow types of compound fertilizers (Urea + Triple super phosphate) and Nitrophoska. Sub-subplots consisted of four doses of each fertilizer. Yield components of wheat which were investigated in this study included number of grains per spike, thousand grains weight, spike index, grain yield and harvest index. In this study the general trend was that the increase in fertilizer dose highly significant increased number of grains per spike in the second season, grain yield in both seasons, but there were no significant difference in thousand grains weight and spike index which were not affected. The results showed that the fertilizer doses significantly decreased harvest index. Generally the results show that there were no significant differences in yield components between the fertilizer types and between the application methods.
试验于2008/2009年和2009/2010年连续两季在苏丹北部州东古拉大学农业科学学院示范农场进行。本试验旨在研究施肥剂量、施肥类型和施肥方式对小麦产量的影响。实验方法为4个重复的分割图试验。主要地块采用随机完全块设计,采用撒播和局部施肥两种施肥方式。小区由两种复合肥(尿素+三元过磷酸钙)和磷硝组成。每个小块由每种肥料的四剂组成。本研究研究的小麦产量成分包括每穗粒数、千粒重、穗指数、籽粒产量和收获指数。在本研究中,总的趋势是施肥剂量的增加极显著提高了第二季的单穗粒数和两季的产量,但千粒重和穗指数差异不显著,未受影响。结果表明,施肥剂量显著降低了收获指数。总体而言,不同肥料类型和施用方式对产量构成没有显著影响。
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引用次数: 4
Physiological and biochemical responses of five nerica rice varieties (Oryza sativa L.) to water deficit at vegetative and reproductive stage 5个籼稻品种营养期和生殖期水分亏缺的生理生化响应
Pub Date : 2012-03-01 DOI: 10.5251/ABJNA.2012.3.3.93.104
P. Sikuku, G. Netondo, J. Onyango
Food shortage is a major problem in sub Saharan Africa as population increases. Over 89% of Kenyans are food poor and are malnourished especially in rural areas. NERICA (New Rice for Africa) are high yielding rainfed rice varieties with early maturity and has shown high potential to revolutionize rice farming even in Africa’s stress afflicted ecologies. However, NERICA varieties vary in their response to water deficit. A pot experiment was conducted in 2009 at the Maseno University Botanic garden, to evaluate the responses of five NERICA varieties (NERICA1, NERICA 2, NERICA 3, NERICA 4 and NERICA 5) to water deficit during their vegetative or reproductive stage of their development. The response pattern of physiological and biochemical parameters to water deficit imposed at different growth stages might provide basis for selecting the most tolerant variety to water deficit in order to stabilize yield and solve food crisis. The treatments were; T1-irrigating the pots with a litre of water after every two days (Control), T2-water deficit at vegetative stage in which water was withheld by irrigating the plants using one litre of water after every six days from 30-50 days after planting; T3-water deficit at reproductive stage in which water was withheld by irrigating the plants using one litre of water after every six days from 51-71 days after planting. Water deficit caused a significant reduction in physiological parameters i.e. growth, chlorophyll fluorescence and biochemical parameters i.e. chlorophyll and protein content both at vegetative stage and at reproductive stage. However, Growth parameters i.e. plant height, root length and plant dry weight were affected more by water deficit imposed at the vegetative stage as compared to water deficit imposed at the reproductive stage while chlorophyll fluorescence, chlorophyll content and protein content were affected more by water deficit imposed at reproductive stage as compared to water deficit at reproductive stage. The results indicate that NERICA 2 and 4 were tolerant as compared to NERICA 1, 3 and 5 to water deficit occurring at vegetative stage or reproductive stage because their growth, chlorophyll fluorescence and biochemical parameters were least affected.
随着人口的增长,粮食短缺是撒哈拉以南非洲的一个主要问题。超过89%的肯尼亚人食物贫乏,营养不良,尤其是在农村地区。NERICA(非洲新水稻)是早熟的高产雨养水稻品种,即使在非洲受压力影响的生态环境中也显示出巨大的潜力,可以彻底改变水稻种植。然而,NERICA品种对缺水的反应各不相同。2009年,在马塞诺大学植物园进行了盆栽试验,研究了5个NERICA品种(NERICA a1、NERICA 2、NERICA 3、NERICA 4和NERICA 5)在营养发育和生殖发育阶段对水分亏缺的响应。不同生育期生理生化参数对水分亏缺的响应规律,可为选择最耐水分亏缺的品种,稳定产量,解决粮食危机提供依据。治疗方法是;t1 -对照每2天灌1升水,t2 -植后30-50天每6天灌1升水,营养期水分亏缺;t3 -生殖期水分亏缺,在种植后51-71天每隔6天灌溉1升水来截留水分。水分亏缺导致营养期和生殖期的生理参数(即生长、叶绿素荧光和生化参数,即叶绿素和蛋白质含量)显著降低。然而,植物生长参数(株高、根长和植株干重)受营养期水分亏缺的影响大于生殖期水分亏缺,叶绿素荧光、叶绿素含量和蛋白质含量受生殖期水分亏缺的影响大于生殖期水分亏缺。结果表明,与NERICA 1、3和5相比,NERICA 2和4对营养期和生殖期水分亏缺的耐受性较好,对其生长、叶绿素荧光和生化指标的影响最小。
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引用次数: 32
Length-Weight relationship and condition factor of tilapia species grown in marine and fresh water ponds 海水和淡水池塘养殖罗非鱼的长重关系及条件因子
Pub Date : 2012-03-01 DOI: 10.5251/ABJNA.2012.3.3.117.124
A. Nehemia, J. D. Maganira, C. Rumisha
The aim of this study was to describe the differences in Length-Weight relationship and condition factor of Tilapia zillii and Oreochromis urolepis urolepis reared in full strength sea water (FSSW) and fresh water (FW) ponds. The environmental condition in FSSW and FW ponds were also analyzed to determine their impacts on Length-Weight relationship and condition factor on both species. The regression coefficient "b" exhibit negative allometric growth in FW and positive allometric growth in FSSW for both species. The value of exponent "b" and condition factor (K) for T. zillii in FW and FSSW (in the bracket) were found to be 2.94 (3.3) and 2.07 (0.74) respectively. On the other hand the value of exponent "b" and condition factor (K) for O. urolepis urolepis in FW and FSSW (in the bracket) were found to be 2.81 (3.46) and 0.86 (0.53) respectively. Though both species performed well in FW, the study also revelead that farming of these tilapia species in FSSW can be feasible if water quality parameters are properly monitored.
本研究旨在探讨全强度海水(FSSW)和淡水(FW)池中养殖的紫罗非鱼(zilliia)和尾鳞罗非鱼(Oreochromis urolepis)的长重关系和条件因子的差异。分析了FSSW和FW池的环境条件,确定了它们对两种物种的长-重关系和条件因子的影响。回归系数“b”均表现为FW负异速生长和FSSW正异速生长。结果表明,FW和FSSW(括号内)的黄僵菌指数b值和条件因子K值分别为2.94(3.3)和2.07(0.74)。另一方面,在FW和FSSW(括号中)中,发现尿鼠的指数b值和条件因子K值分别为2.81(3.46)和0.86(0.53)。虽然这两种罗非鱼在淡水中表现良好,但研究还表明,如果对水质参数进行适当监测,在淡水中养殖这些罗非鱼是可行的。
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引用次数: 120
The effects of green manure and NPK fertilizer on the growth and yield of maize (Zea mays L) in the mount Cameroon region 绿肥和氮磷钾肥对喀麦隆山区玉米生长和产量的影响
Pub Date : 2012-03-01 DOI: 10.5251/ABJNA.2012.3.3.82.92
E. Egbe, B. Fonge
This study was carried out in the Mount Cameroon region to evaluate the effects of green manure and fertilizer on the growth and yield of maize. There were eight treatments: Calliandra prunings, Calliandra prunings + fertilizer (60kg/ha NPK), Gliricidia prunings, Gliricidia prunings + fertilizer, Senna prunings, Senna prunings + fertilizer, fertilizer only (120 kg/ha NPK) and control. Results showed that biomass of prunings in the first pruning was highest in Calliandra (5314kg/ha) and this was significantly different from those of Senna (2909kg/ha) and Gliricidia (2112kg/ha). Nitrogen input from the prunings was highly significant (P<0.05) in Calliandra prunings (217 kgN/ha) and least in Gliricidia species (69.0kgN/ha). Maize grain yield in the different treatments was not significantly different in the first and second planting seasons. The highest grain yield during the third cropping season was observed in Calliandra + fertilizer (4696kg/ha) and least in control (3332kg/ha). It was also noted that nitrogen uptake was highest in plots supplied with fertilizer only and lowest in control. Weed biomass was highest in control and in plots supplied with fertilizer only. Soil chemical changes after three cropping periods showed variable changes in treatments. There was decrease in bulk density in plots supplied with Calliandra prunings.
本研究在喀麦隆山地区进行,以评价绿肥和化肥对玉米生长和产量的影响。试验分为花椒修剪、花椒修剪+肥料(60kg/ hnpk)、花椒修剪、花椒修剪+肥料、番泻修剪、番泻修剪+肥料、仅施肥(120kg / hnpk)和对照8个处理。结果表明:第一次剪枝生物量最高的是花椒属植物(5314kg/ha),显著高于Senna属植物(2909kg/ha)和Gliricidia属植物(2112kg/ha);花椒属(Calliandra)的氮素输入极显著(P<0.05),为217 kgN/ha,花椒属(gliriicidia)的氮素输入最低,为69.0kgN/ha。不同处理的玉米产量在第一、第二种植季差异不显著。第三季籽粒产量最高的是花椒配肥(4696kg/ha),最低的是对照(3332kg/ha)。还注意到,氮素吸收量在只施用肥料的地块最高,而对照地块最低。对照和只施肥小区的杂草生物量最高。3个生育期后土壤化学变化在不同处理中表现出不同的变化。在次生木修剪的地块上,容重有所下降。
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引用次数: 0
Effect of ammonium salts on the biodegradation of used transformer oil using locally isolated microorganisms 铵盐对局部分离微生物降解废变压器油的影响
Pub Date : 2012-03-01 DOI: 10.5251/ABJNA.2012.3.3.131.139
A. Ayandele, O. Fagade
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引用次数: 3
In vitro antimicrobial and cytotoxicity screening of n-hexane, chloroform and ethyl acetate extracts of Lablab purpureus (L.) leaves 紫荆叶正己烷、氯仿和乙酸乙酯提取物的体外抗菌和细胞毒性筛选
Pub Date : 2012-02-01 DOI: 10.5251/ABJNA.2012.3.2.43.48
F. Nasrin, I. Bulbul, Y. Begum, Sayema Khanum
In the present study the antimicrobial & cytotoxic activity of crude extracts (Chloroform, n-Hexane, Ethyl acetate) of leaves of Lablab purpureus L. were studied. Antimicrobial activity was tested against eleven important pathogenic bacteria including both gram positive and gram negative bacteria and three fungi. The bacteria are B. megaterium, B. subtilis, Staphylococcus aureus, Sarcina lutea, Escherichia coli, Salmonella paratyphi, S. typhi, Shigella boydii, S. dysenteriae and Vibrio mimicus & V. parahemolyticus. Disc diffusion technique was used for invitro antibacterial and antifungal screening. Here kanamycin disc (30g /disc) was used as standard for antibacterial study. The extracts showed antimicrobial activity against most of the bacterial strains with an average zone of inhibition of 8-20mm. The tested fungi are Saccharromyces cerevaceae, Candida albicans and Aspergillus niger. The extracts showed moderate to good antifungal activity with an average 9 -15 mm zone of inhibition. Among the three solvent extracts used, the most effective extract was found to be n-Hexane extract and maximum activity (20 mm, zone of inhibition) found against Staphylococcus aureus with Minimum inhibitory concentration (MIC) values of 64g/ml. The maximum zone of inhibition for chloroform extract showed 17mm against Bacillus subtilis and E.coli with MIC 128g/ml and 32g/ml respectively. The maximum zone of inhibition for Ethyl acetate extract showed 17mm against Vibrio mimicus with MIC values of 64g/ml .Cytotoxicity test was also studied by Brine Shrimp Lethality Bioassay and compare with LC50 values of standard Vincristin sulphate as a positive control. The results illustrated significant cytotoxicity against A. salina, with LC50 13.88µg/ml, 19.17µg/ml and 17.97µg/ml for n-Hexane, Chloroform and Ethyl acetate extracts respectively.
本文研究了紫花Lablab purpureus L.叶片粗提物(氯仿、正己烷、乙酸乙酯)的抑菌和细胞毒活性。对革兰氏阳性菌和革兰氏阴性菌等11种重要病原菌及3种真菌进行了抑菌活性试验。这些细菌是巨型芽孢杆菌、枯草芽孢杆菌、金黄色葡萄球菌、黄斑肉球菌、大肠杆菌、副伤寒沙门氏菌、伤寒沙门氏菌、博氏志贺氏菌、痢疾沙门氏菌和模拟弧菌和副溶血性弧菌。采用圆盘扩散技术进行体外抗菌和抗真菌筛选。以卡那霉素片(30g /片)作为抑菌标准。提取液对大部分病原菌均有抑菌活性,平均抑菌区为8 ~ 20mm。测试的真菌是酿酒酵母菌、白色念珠菌和黑曲霉。提取物具有中等至良好的抗真菌活性,平均抑制区为9 ~ 15 mm。三种溶剂提取物中,正己烷提取物对金黄色葡萄球菌的抑制作用最强(20 mm,抑制区),最小抑制浓度(MIC)为64g/ml。氯仿提取物对枯草芽孢杆菌和大肠杆菌的最大抑制区为17mm, MIC分别为128g/ml和32g/ml。乙酸乙酯提取物对mimicus弧菌的最大抑制区为17mm, MIC值为64g/ml。采用卤虾致死性生物测定法进行细胞毒性试验,并与标准硫酸长春新素LC50值作阳性对照。结果表明,正己烷、氯仿和乙酸乙酯提取物的LC50分别为13.88µg/ml、19.17µg/ml和17.97µg/ml。
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引用次数: 11
Ecological study of four populations acridians (Insecta Orthiptera) areaof El-aouana (Jijel-Algeria) 阿尔及利亚jjel -aouana地区4个蝗科(直翅目)种群生态学研究
Pub Date : 2012-02-01 DOI: 10.5251/ABJNA.2012.3.2.57.68
F. Tekkouk
The study focuses on Acridoidea Orthoptera recorded in four stations represented in one of the largest sites of the Algerian EL-AOUANA (JIJEL) coastline. The work carried out by sampling from May to December 2006 showed the presence of 22 species in 10 families. The similarity and distance matrices indicate that station fact sheet (S1) is almost similar with the (S3) fallow and cultivated environment (S4) .about the basis of ranks frequencies, the vegetation distribution diagrams shows the existence of four groups of locust corresponding to the 4 studied stations. The analysis of the levels for the resulting integration of the classification of the stations shows that can hold two separate groups of stations.
该研究的重点是在阿尔及利亚EL-AOUANA (JIJEL)海岸线最大的一个地点的四个站点记录的直翅蝗科。2006年5月至12月的采样工作表明,在10科中存在22种。相似矩阵和距离矩阵表明,站点概况(S1)与休耕环境(S4)基本相似,在等级频次的基础上,植被分布图显示4个站点对应的蝗虫存在4个类群。对所产生的台站分类整合的水平分析表明,可以容纳两个独立的台站组。
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引用次数: 3
Pharmacological action of integument extracts of red palm weevil, Rhynchophorus ferrugineus on albino mice 红棕象鼻虫被皮提取物对白化小鼠的药理作用
Pub Date : 2012-02-01 DOI: 10.5251/ABJNA.2012.3.2.49.56
M. Al-Dosary, K. Tahir
Extracts were prepared from the tergum and sternum of male and female red palm weevil, Rhynchophorus ferrugineus, using acetone, chloroform and diethyl ether. Pharmacological actions were examined in mice. All of the extracts showed certain behavioral actions that included central nervous system inhibition and stimulation with no effects on pain perception or temperature regulation.Two of the extracts labeled numbers 1 and 2 (acetone and chloroform extracts of the female insect tergum) were found to exhibit cardiac depressant effects. Extract No.1 induced time- dependent bradycardia ( up to 40% inhibition of heart rate) that was significantly antagonized by atropine. It also enhanced calcium chloride- induced bradycardia in mice. Insect extract No.2 also exhibited cardiac depressant effect (up to 37.5%bradycardia) that was not affected by atropine.The extract significantly blocked calcium chloride-induced bradycardia. Extracts No.1 and 2 seemed to contain muscarinic and calcium channel bloking components, respectively. Isolation of these components may pave the way for discovery of potential cardiac depressants that may be useful in treatment of certain cardiac dysrrhythmias.
以红棕象鼻虫(Rhynchophorus ferrugineus)雄性和雌性的尾胶和胸骨为原料,用丙酮、氯仿和乙醚制备了提取物。对小鼠进行了药理作用研究。所有的提取物都表现出一定的行为作用,包括中枢神经系统的抑制和刺激,而对痛觉或温度调节没有影响。标记为1和2的两种提取物(雌性昆虫三叶胶的丙酮和氯仿提取物)被发现具有抑制心脏的作用。提取物1诱导时间依赖性心动过缓(高达40%的心率抑制),被阿托品显著拮抗。它还能增强小鼠氯化钙诱导的心动过缓。昆虫提取物2号也表现出心脏抑制作用(高达37.5%的心动过缓),不受阿托品的影响。该提取物可显著阻断氯化钙诱导的心动过缓。提取物1和2似乎分别含有毒蕈碱和钙通道阻断成分。这些成分的分离可能为发现潜在的心脏抑制剂铺平道路,这些抑制剂可能对治疗某些心律失常有用。
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引用次数: 1
Genetic variability of body composition traits in Sudanese Native large Beladi Chicken 苏丹本土大贝拉迪鸡体组成性状的遗传变异
Pub Date : 2012-02-01 DOI: 10.5251/ABJNA.2012.3.2.69.76
A. Khalid, Ibrahim Saeed Yousif, Mahmoud I Omer, K. Elamin
This work was conducted to estimate the genetic and phenotypic parameters for body weight, carcass traits and visceral portions of the native large Beladi chicken. Data set consisted of 1114 observations obtained from 50 sires and 150 dams were analyzed using Harvey’s SLMLMW (1990) to obtain variance and covariance components for genetic parameters estimates. The average body weight at twelve weeks of age (800.74± 162.98g) was lower than that of exotic strains at slaughter age. The results exhibited significant differences between sexes for all traits measured with males being heavier than females. As proportion of body weight at 12 weeks of age the hot carcass weight was 63.30 percent, whereas breast, thigh, drum and leg constituted 24.60, 15.50, 14.70 and 30.10 percents of the cold carcass weight respectively. Moreover, the abdominal fat, gizzard, heart, liver and shanks represented 0.72, 2.4, 0.55, 2.38 and 4.04 percents of live body weight at 12 weeks of age respectively. The heritability estimates for body weight at 12 weeks of age and carcass traits ranged from moderate to high. This indicates the possibility of improving these traits in local chicken using selection procedures. The heritability estimates for visceral portions were low. The genetic correlation estimates for body weight and carcass traits, body weight and abdominal fat and carcass weight and abdominal fat were high and positive. Therefore attempts to improve body weight in indigenous chicken may lead to concomitant increase in carcass traits including abdominal fat. Keyword: Body composition, Genetic variability, Native chicken.
本研究旨在估计本地大贝拉迪鸡的体重、胴体性状和内脏部分的遗传和表型参数。利用Harvey 's SLMLMW(1990)分析了来自50个站点和150个水坝的1114个观测数据集,获得了遗传参数估计的方差和协方差成分。12周龄平均体重(800.74±162.98g)低于屠宰龄外来菌株。结果显示,男性比女性重的所有特征在性别之间都存在显著差异。热胴体重占12周龄体重的63.30%,胸、腿、鼓和腿分别占冷胴体重的24.60%、15.50%、14.70%和30.10%。腹部脂肪、砂囊脂肪、心脏脂肪、肝脏脂肪和小腿脂肪分别占12周龄活重的0.72%、2.4、0.55%、2.38%和4.04%。12周龄体重和胴体性状的遗传力估计从中等到高。这表明利用选择程序改善地方鸡这些性状的可能性。内脏部分的遗传率估计很低。体重与胴体性状、体重与腹部脂肪、胴体体重与腹部脂肪的遗传相关性较高且呈正相关。因此,试图改善土鸡体重可能会导致胴体性状(包括腹部脂肪)的增加。关键词:体成分,遗传变异,土鸡。
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引用次数: 8
期刊
Agriculture and Biology Journal of North America
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