{"title":"MT May 2023 Industry & Product News","authors":"","doi":"10.1093/mictod/qaad022","DOIUrl":"https://doi.org/10.1093/mictod/qaad022","url":null,"abstract":"","PeriodicalId":74194,"journal":{"name":"Microscopy today","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46406490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Pollinators Have Been Around for a Very Long Time!","authors":"S. Carmichael","doi":"10.1093/mictod/qaad020","DOIUrl":"https://doi.org/10.1093/mictod/qaad020","url":null,"abstract":"","PeriodicalId":74194,"journal":{"name":"Microscopy today","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47181803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A non-local-means algorithm with non-local averaging is used to reduce noise in an electron holography phase map. The noise level is evaluated through wavelet analysis. The method is shown to be effective in reducing noise in the map while preserving both the spatial resolution and underlying map information. Because of the significant noise reduction, the processed phase map is easier to understand and can produce an internal electric field map with a derivative of the phase map.
{"title":"Noise Reduction of Electron Holography Phase Maps with Non-Local-Mean Wavelet Analysis","authors":"Shixin Wang, Yun-Yu Wang","doi":"10.1093/mictod/qaad026","DOIUrl":"https://doi.org/10.1093/mictod/qaad026","url":null,"abstract":"\u0000 A non-local-means algorithm with non-local averaging is used to reduce noise in an electron holography phase map. The noise level is evaluated through wavelet analysis. The method is shown to be effective in reducing noise in the map while preserving both the spatial resolution and underlying map information. Because of the significant noise reduction, the processed phase map is easier to understand and can produce an internal electric field map with a derivative of the phase map.","PeriodicalId":74194,"journal":{"name":"Microscopy today","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44330254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Immunohistochemistry (IHC) is the use of antibodies as probes to determine the localization of proteins in a cell or tissue sample. In microscopy, the technique has been in use since the 1940s by pathologists and research scientists. At first glance it appears a straightforward procedure. However, in practice, an IHC protocol has a number of steps where problems can occur, resulting in either false positive or false negative data. IHC can be used in both transmitted light and fluorescence microscopy, and it can be used in combinations to identify and mark multiple proteins of interest. While the technique has been published extensively, a certain amount of confusion exists as to how to optimize it. This series of three articles describes the steps involved in IHC, their function, and some variations that occur in specific protocols on differing cell and tissue types. It is hoped that a better understanding of these steps will help to guide users in determining the optimal conditions for carrying through IHC protocols.
{"title":"Using Antibodies in Microscopy: A Guide to Immunohistochemistry. Part 2: IHC Staining Protocols","authors":"C. Guerin","doi":"10.1093/mictod/qaad029","DOIUrl":"https://doi.org/10.1093/mictod/qaad029","url":null,"abstract":"\u0000 Immunohistochemistry (IHC) is the use of antibodies as probes to determine the localization of proteins in a cell or tissue sample. In microscopy, the technique has been in use since the 1940s by pathologists and research scientists. At first glance it appears a straightforward procedure. However, in practice, an IHC protocol has a number of steps where problems can occur, resulting in either false positive or false negative data. IHC can be used in both transmitted light and fluorescence microscopy, and it can be used in combinations to identify and mark multiple proteins of interest. While the technique has been published extensively, a certain amount of confusion exists as to how to optimize it. This series of three articles describes the steps involved in IHC, their function, and some variations that occur in specific protocols on differing cell and tissue types. It is hoped that a better understanding of these steps will help to guide users in determining the optimal conditions for carrying through IHC protocols.","PeriodicalId":74194,"journal":{"name":"Microscopy today","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47612635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Pioneers in Optics: Robert Wilhelm Bunsen (1811–1899)","authors":"Cameron Varano Casasanta","doi":"10.1093/mictod/qaad025","DOIUrl":"https://doi.org/10.1093/mictod/qaad025","url":null,"abstract":"","PeriodicalId":74194,"journal":{"name":"Microscopy today","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41314472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"What Will Microscopy Look Like in 10 Years?","authors":"Robert L. Price","doi":"10.1093/mictod/qaad032","DOIUrl":"https://doi.org/10.1093/mictod/qaad032","url":null,"abstract":"","PeriodicalId":74194,"journal":{"name":"Microscopy today","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42318679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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