{"title":"Microanalysis Society Awards: 2023 Award Recipients","authors":"Bradley T De Gregorio, Pat Camus","doi":"10.1093/mictod/qaad045","DOIUrl":"https://doi.org/10.1093/mictod/qaad045","url":null,"abstract":"","PeriodicalId":74194,"journal":{"name":"Microscopy today","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135568283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Atomic force microscopes (AFMs) have emerged as the principal enabling tool for nanotechnology research. They are used ubiquitously in a wide range of fields: from 2D materials, semiconductors, ferroelectrics, and batteries to biomolecules, polymers, and cell biology. As the name implies, AFMs are microscopes. However, rather than using focused light or electrons to magnify sample features, AFMs scan a mechanical probe with a very sharp tip over the surface to create a high-resolution 3D topographical image. Further, by modifying the probe composition or structure, other material properties (electrical, mechanical, magnetic, etc.) can be simultaneously measured and mapped onto the topographic image for precise structure/property correlation. Clearly, the probe is key to unlocking the power of the AFM, thus, choosing the right probe is critical. In this article, we will provide novice and experienced users with basic information and guidelines to simplify the AFM probe selection process.
{"title":"How to Choose the Right AFM Probe for Your Experiment","authors":"F Ted Limpoco, David E Beck","doi":"10.1093/mictod/qaad055","DOIUrl":"https://doi.org/10.1093/mictod/qaad055","url":null,"abstract":"Abstract Atomic force microscopes (AFMs) have emerged as the principal enabling tool for nanotechnology research. They are used ubiquitously in a wide range of fields: from 2D materials, semiconductors, ferroelectrics, and batteries to biomolecules, polymers, and cell biology. As the name implies, AFMs are microscopes. However, rather than using focused light or electrons to magnify sample features, AFMs scan a mechanical probe with a very sharp tip over the surface to create a high-resolution 3D topographical image. Further, by modifying the probe composition or structure, other material properties (electrical, mechanical, magnetic, etc.) can be simultaneously measured and mapped onto the topographic image for precise structure/property correlation. Clearly, the probe is key to unlocking the power of the AFM, thus, choosing the right probe is critical. In this article, we will provide novice and experienced users with basic information and guidelines to simplify the AFM probe selection process.","PeriodicalId":74194,"journal":{"name":"Microscopy today","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135509280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Dr. Dale Newbury: An Impressive 50-Year Career and Legacy","authors":"Cameron Varano Casasanta","doi":"10.1093/mictod/qaad051","DOIUrl":"https://doi.org/10.1093/mictod/qaad051","url":null,"abstract":"","PeriodicalId":74194,"journal":{"name":"Microscopy today","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41996739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Okerstrom, D. Esponda, Carolina Fernandez-Jansink
This article proposes using microscopy for nature-inspired design in the early stage of “fuzzy front end” radical innovation and sustainable design technology, where it would have a high positive or sustainable impact. To give a taste of what is possible, a live virtual Nature Experience Radical Design (NERD) Jam was created. Instead of starting with a problem, designers, biologists, engineers, microscopists, and curious people were invited to begin with the solution. By observing and empathizing with nature, both in its element and under a microscope, microscopy reveals unique structural adaptations and possible unknown strategies and mechanisms when exploring nature as a series of successful devices in particular environmental contexts. By looking at nature on multiple scales, one can discover unique models to design sustainable solutions for multiple human challenges.
{"title":"Virtual Microscopy – Nature Experience Radical Design (NERD) Jam","authors":"S. Okerstrom, D. Esponda, Carolina Fernandez-Jansink","doi":"10.1093/mictod/qaad054","DOIUrl":"https://doi.org/10.1093/mictod/qaad054","url":null,"abstract":"\u0000 This article proposes using microscopy for nature-inspired design in the early stage of “fuzzy front end” radical innovation and sustainable design technology, where it would have a high positive or sustainable impact. To give a taste of what is possible, a live virtual Nature Experience Radical Design (NERD) Jam was created. Instead of starting with a problem, designers, biologists, engineers, microscopists, and curious people were invited to begin with the solution. By observing and empathizing with nature, both in its element and under a microscope, microscopy reveals unique structural adaptations and possible unknown strategies and mechanisms when exploring nature as a series of successful devices in particular environmental contexts. By looking at nature on multiple scales, one can discover unique models to design sustainable solutions for multiple human challenges.","PeriodicalId":74194,"journal":{"name":"Microscopy today","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43476549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract The National Institutes of Health (NIH) implemented a new Data Management and Sharing Policy on January 25, 2023. This policy replaces the 2003 document and affirms the NIH’s support for open science and data sharing. Almost all proposals for research that will produce scientific data are now required to include a Data Management and Sharing Plan, which lays out how the research team will collect and preserve data to ensure long-term discoverability and access. Researchers must approach their projects with data sharing in mind, to the extent that is ethically, legally, and technically possible.
{"title":"Complying with the New NIH Data Management and Sharing Policy","authors":"Stacy Winchester","doi":"10.1093/mictod/qaad036","DOIUrl":"https://doi.org/10.1093/mictod/qaad036","url":null,"abstract":"Abstract The National Institutes of Health (NIH) implemented a new Data Management and Sharing Policy on January 25, 2023. This policy replaces the 2003 document and affirms the NIH’s support for open science and data sharing. Almost all proposals for research that will produce scientific data are now required to include a Data Management and Sharing Plan, which lays out how the research team will collect and preserve data to ensure long-term discoverability and access. Researchers must approach their projects with data sharing in mind, to the extent that is ethically, legally, and technically possible.","PeriodicalId":74194,"journal":{"name":"Microscopy today","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135509282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Digital imaging has made scientific light microscope imaging much faster and facilitates a range of imaging techniques not previously available. Advances in microscope digital image detection systems over the last decade have played an integral part in establishing digital imaging as the medium of choice for most types of microscopy. Although we still refer to them as cameras, in actuality, current detector systems constitute specific hardware and software components specifically adapted for specific types of microscopy. Although these advances have facilitated data collection with microscopes, the plethora of choices comes with the need to understand which system best suits a specific researcher’s needs. In an effort to help navigate the multiple choices available, this article and a subsequent one will list what I feel are the major parameters to use in the evaluation of different detection systems, describe the major categories of cameras available, and discuss the advantages and pitfalls of each digital image solution.
{"title":"Choosing the Best Camera System for Your Biological Light Microscopy Needs: Part I","authors":"W. G. (. Jerome","doi":"10.1093/mictod/qaad039","DOIUrl":"https://doi.org/10.1093/mictod/qaad039","url":null,"abstract":"\u0000 Digital imaging has made scientific light microscope imaging much faster and facilitates a range of imaging techniques not previously available. Advances in microscope digital image detection systems over the last decade have played an integral part in establishing digital imaging as the medium of choice for most types of microscopy. Although we still refer to them as cameras, in actuality, current detector systems constitute specific hardware and software components specifically adapted for specific types of microscopy. Although these advances have facilitated data collection with microscopes, the plethora of choices comes with the need to understand which system best suits a specific researcher’s needs. In an effort to help navigate the multiple choices available, this article and a subsequent one will list what I feel are the major parameters to use in the evaluation of different detection systems, describe the major categories of cameras available, and discuss the advantages and pitfalls of each digital image solution.","PeriodicalId":74194,"journal":{"name":"Microscopy today","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42231389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Index of Advertisers","authors":"","doi":"10.1093/mictod/qaad058","DOIUrl":"https://doi.org/10.1093/mictod/qaad058","url":null,"abstract":"","PeriodicalId":74194,"journal":{"name":"Microscopy today","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135568408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Immunohistochemistry (IHC) is the use of antibodies as probes to determine the localization of proteins in a cell or tissue sample. In microscopy, the technique has been in use since the 1940s by pathologists and research scientists. At first glance it appears a straightforward procedure. In practice, however, an IHC protocol has many steps where problems can occur resulting in either false positive or false negative data. IHC can be used for ultrastructural studies using electron microscopy (EM), but the protocols used for light microscopy must be adapted. While IHC techniques have been published extensively, a certain amount of confusion exists as to how to optimize them. This series of articles describes the steps involved in IHC and their function, and some variations that occur in specific protocols on differing cell and tissue types. It is hoped that a better understanding of these steps will help to guide users in determining the optimal conditions for carrying through IHC protocols.
{"title":"Using Antibodies in Microscopy: A Guide to Immunohistochemistry. Part 3: Post-Embedding Electron Microscopy Techniques","authors":"Christopher J Guerin","doi":"10.1093/mictod/qaad050","DOIUrl":"https://doi.org/10.1093/mictod/qaad050","url":null,"abstract":"Abstract Immunohistochemistry (IHC) is the use of antibodies as probes to determine the localization of proteins in a cell or tissue sample. In microscopy, the technique has been in use since the 1940s by pathologists and research scientists. At first glance it appears a straightforward procedure. In practice, however, an IHC protocol has many steps where problems can occur resulting in either false positive or false negative data. IHC can be used for ultrastructural studies using electron microscopy (EM), but the protocols used for light microscopy must be adapted. While IHC techniques have been published extensively, a certain amount of confusion exists as to how to optimize them. This series of articles describes the steps involved in IHC and their function, and some variations that occur in specific protocols on differing cell and tissue types. It is hoped that a better understanding of these steps will help to guide users in determining the optimal conditions for carrying through IHC protocols.","PeriodicalId":74194,"journal":{"name":"Microscopy today","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135509281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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