Pub Date : 2015-07-23DOI: 10.4172/2161-1068.1000188
Monika Gupta, M. Mittal, A. Agarwal, B. Thukral
Primary tubercular osteomyelitis of sternum is a rare clinical entity and involvement of manubrio-sternal joint is even rarer, with only few cases reported in the literature. Tuberculous sternal involvement is seen in approximately 1% of all skeletal TB cases. We report an unusual case of primary manubrio-sternal joint tuberculosis presenting as vague anterior chest wall swelling and pain. ESR and CRP were elevated, Montoux was positive and PCR for tuberculosis was positive. Computed tomography of chest done after giving intravenous contrast showed erosion at manubrio-sternal joint with minimal soft tissue component. The patient is put on anti-tubercular drugs and is responding well with regular follow-up.
{"title":"Primary Tubercular Osteomyelitis of Manubrio Sternal Joint: A Case Report","authors":"Monika Gupta, M. Mittal, A. Agarwal, B. Thukral","doi":"10.4172/2161-1068.1000188","DOIUrl":"https://doi.org/10.4172/2161-1068.1000188","url":null,"abstract":"Primary tubercular osteomyelitis of sternum is a rare clinical entity and involvement of manubrio-sternal joint is even rarer, with only few cases reported in the literature. Tuberculous sternal involvement is seen in approximately 1% of all skeletal TB cases. We report an unusual case of primary manubrio-sternal joint tuberculosis presenting as vague anterior chest wall swelling and pain. ESR and CRP were elevated, Montoux was positive and PCR for tuberculosis was positive. Computed tomography of chest done after giving intravenous contrast showed erosion at manubrio-sternal joint with minimal soft tissue component. The patient is put on anti-tubercular drugs and is responding well with regular follow-up.","PeriodicalId":74235,"journal":{"name":"Mycobacterial diseases : tuberculosis & leprosy","volume":"5 1","pages":"1-3"},"PeriodicalIF":0.0,"publicationDate":"2015-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70581228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-07-15DOI: 10.4172/2161-1068.1000187
D. Ijdema, C. Magis-Escurra, Horsting Pp, C. Erkens, R. Aarnoutse, M. Boeree
Introduction: With declining TB incidences diagnostic delays, especially in extra-pulmonary TB, may increase. We describe the patient and clinical characteristics of patients with spinal TB and assessed the course of diagnostic delays of spinal TB from 2000-2011 in The Netherlands. Methods: Data from the Netherlands Tuberculosis Registry were studied, completed with basic demographic data and data considering patients-, doctors- and total diagnostic delay retrieved from the patient records at the public municipal health services. Results: A total of 274 cases were studied. Median diagnostic delay was five months and stable during this period. Sex and age groups were associated with significant differences in diagnostic delay (male 4.5 vs female 5.5 months), and 4.5-5 months in the youngest age group and persons>65 years but 5.75 months in patients aged 35-64 years. No difference was observed between origin of patients, patients presenting with TB risk factors or with neurological symptoms. Typical TB symptoms at presentation lead, surprisingly, to significantly increased doctors’ delay (typical symptoms 4.0 vs no typical symptoms 2.0 months, p=0.05). Conclusion: Considering spinal TB diagnosis and act expeditious is necessary to limit the time to diagnosis in spinal TB. Refresher courses should be offered both to family physicians and clinical specialists in The Netherlands
{"title":"Clinical Characteristics and Diagnostic Delay in Spinal Tuberculosis Patients in The Netherlands","authors":"D. Ijdema, C. Magis-Escurra, Horsting Pp, C. Erkens, R. Aarnoutse, M. Boeree","doi":"10.4172/2161-1068.1000187","DOIUrl":"https://doi.org/10.4172/2161-1068.1000187","url":null,"abstract":"Introduction: With declining TB incidences diagnostic delays, especially in extra-pulmonary TB, may increase. We describe the patient and clinical characteristics of patients with spinal TB and assessed the course of diagnostic delays of spinal TB from 2000-2011 in The Netherlands. \u0000Methods: Data from the Netherlands Tuberculosis Registry were studied, completed with basic demographic data and data considering patients-, doctors- and total diagnostic delay retrieved from the patient records at the public municipal health services. \u0000Results: A total of 274 cases were studied. Median diagnostic delay was five months and stable during this period. Sex and age groups were associated with significant differences in diagnostic delay (male 4.5 vs female 5.5 months), and 4.5-5 months in the youngest age group and persons>65 years but 5.75 months in patients aged 35-64 years. No difference was observed between origin of patients, patients presenting with TB risk factors or with neurological symptoms. Typical TB symptoms at presentation lead, surprisingly, to significantly increased doctors’ delay (typical symptoms 4.0 vs no typical symptoms 2.0 months, p=0.05). \u0000Conclusion: Considering spinal TB diagnosis and act expeditious is necessary to limit the time to diagnosis in spinal TB. Refresher courses should be offered both to family physicians and clinical specialists in The Netherlands","PeriodicalId":74235,"journal":{"name":"Mycobacterial diseases : tuberculosis & leprosy","volume":"5 1","pages":"1000187"},"PeriodicalIF":0.0,"publicationDate":"2015-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70581175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-06-05DOI: 10.4172/2161-1068.1000185
Y. Kobashi, K. Mouri, Shigeki Kato, M. Oka
Background: The aim of this study was to evaluate the clinical potential of IP-10 and MIG as biomarkers of tuberculosis (TB) infection adding a comparison with IGRAs (QFT and T-SPOT.TB). Materials and Methods: The subjects consisted of 52 patients with active TB disease and 86 patients with non-TB disease. We measured two IGRAs using peripheral blood (PB), and IP-10 and MIG using the supernatant from whole blood stimulated with MTB (Mycobacterium tuberculosis)-specific antigens. Results: In the patient group with active TB disease, while the positive response rates of QFT and T-SPOT.TB were 81% and 87%, that of IP-10 using the supernatant was 88% and that of MIG was 85%. In the patient group with non-TB disease, the positive response rate of QFT was 13%, and that of T-SPOT.TB was 14%, IP-10 using the supernatant was 14%, and that of MIG was 14%. The IP-10 and MIG levels of the patients with active TB disease using the supernatant were significantly higher than those of the patients with non-TB disease. The combination of four diagnostic methods using the supernatant increased the positive response rate to 94%. Conclusion: IP-10 and MIG using a supernatant stimulated with MTB-specific antigens showed similar results to IGRAs. Therefore, these tests can be used as alternatives for the diagnosis of active TB disease.
{"title":"Clinical Evaluation of IP-10 and MIG for the Diagnosis of Active Tuberculosis Disease","authors":"Y. Kobashi, K. Mouri, Shigeki Kato, M. Oka","doi":"10.4172/2161-1068.1000185","DOIUrl":"https://doi.org/10.4172/2161-1068.1000185","url":null,"abstract":"Background: The aim of this study was to evaluate the clinical potential of IP-10 and MIG as biomarkers of tuberculosis (TB) infection adding a comparison with IGRAs (QFT and T-SPOT.TB). \u0000Materials and Methods: The subjects consisted of 52 patients with active TB disease and 86 patients with non-TB disease. We measured two IGRAs using peripheral blood (PB), and IP-10 and MIG using the supernatant from whole blood stimulated with MTB (Mycobacterium tuberculosis)-specific antigens. \u0000Results: In the patient group with active TB disease, while the positive response rates of QFT and T-SPOT.TB were 81% and 87%, that of IP-10 using the supernatant was 88% and that of MIG was 85%. In the patient group with non-TB disease, the positive response rate of QFT was 13%, and that of T-SPOT.TB was 14%, IP-10 using the supernatant was 14%, and that of MIG was 14%. The IP-10 and MIG levels of the patients with active TB disease using the supernatant were significantly higher than those of the patients with non-TB disease. The combination of four diagnostic methods using the supernatant increased the positive response rate to 94%. \u0000Conclusion: IP-10 and MIG using a supernatant stimulated with MTB-specific antigens showed similar results to IGRAs. Therefore, these tests can be used as alternatives for the diagnosis of active TB disease.","PeriodicalId":74235,"journal":{"name":"Mycobacterial diseases : tuberculosis & leprosy","volume":"5 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2015-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4172/2161-1068.1000185","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70581528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-05-28DOI: 10.4172/2161-1068.1000184
C. Dassi, L. Mosi, B. Akpatou, Charles Akugbey Narh, Charles Quaye, D. O. Konan, J. Djaman, B. Bonfoh
The endemicity of Buruli ulcer (BU), a non-tuberculous mycobacteria infection, has significantly increased in Cote d'Ivoire. The exact transmission mode remains unknown but DNA based evidence of Mycobacterium ulcerans, the causative agent, have implicated potential environmental reservoirs, similar to those suspected in the transmission to humans. The role of small mammals in transmission has recently received some research attention. Based on the hypothesis that the overlapping ecology of human and animal habitats would favour mycobacteria transmission, the study aimed to identify BU like infections in small mammals living in close proximity to humans, in endemic communities. One hundred and eleven animals were trapped within five communities in two major endemic areas, Daloa and Tabbo. Majority of trapped small mammals were mice, Mastomys natalensis, of which 8 animals had external lesions. PCR on organ and lesion samples identified, predominantly, mycobacterium spp, of which five mice were positive for IS2404. IS2404 sequencing confirmed infection in two mice as M. ulcerans strain Agy99. Our findings suggest that small terrestrial mammals could be susceptible to mycobacteria, particularly, M. ulcerans, within endemic communities in Cote d’Ivoire. It also consolidates earlier evidence asserting their role as potential environmental reservoirs of M. ulcerans in endemic communities. The potential public health threat from these small mammals warrants an “OneHealth” approach to investigating the ecology and transmission of M. ulcerans in endemic countries.
{"title":"Detection of Mycobacterium ulcerans in Mastomys natalensis and PotentialTransmission in Buruli ulcer Endemic Areas in Côte d'Ivoire","authors":"C. Dassi, L. Mosi, B. Akpatou, Charles Akugbey Narh, Charles Quaye, D. O. Konan, J. Djaman, B. Bonfoh","doi":"10.4172/2161-1068.1000184","DOIUrl":"https://doi.org/10.4172/2161-1068.1000184","url":null,"abstract":"The endemicity of Buruli ulcer (BU), a non-tuberculous mycobacteria infection, has significantly increased in Cote d'Ivoire. The exact transmission mode remains unknown but DNA based evidence of Mycobacterium ulcerans, the causative agent, have implicated potential environmental reservoirs, similar to those suspected in the transmission to humans. The role of small mammals in transmission has recently received some research attention. Based on the hypothesis that the overlapping ecology of human and animal habitats would favour mycobacteria transmission, the study aimed to identify BU like infections in small mammals living in close proximity to humans, in endemic communities. One hundred and eleven animals were trapped within five communities in two major endemic areas, Daloa and Tabbo. Majority of trapped small mammals were mice, Mastomys natalensis, of which 8 animals had external lesions. PCR on organ and lesion samples identified, predominantly, mycobacterium spp, of which five mice were positive for IS2404. IS2404 sequencing confirmed infection in two mice as M. ulcerans strain Agy99. Our findings suggest that small terrestrial mammals could be susceptible to mycobacteria, particularly, M. ulcerans, within endemic communities in Cote d’Ivoire. It also consolidates earlier evidence asserting their role as potential environmental reservoirs of M. ulcerans in endemic communities. The potential public health threat from these small mammals warrants an “OneHealth” approach to investigating the ecology and transmission of M. ulcerans in endemic countries.","PeriodicalId":74235,"journal":{"name":"Mycobacterial diseases : tuberculosis & leprosy","volume":"31 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2015-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70581249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-05-25DOI: 10.4172/2161-1068.1000182
M. Tungdim, Heemanshu Aurora, S. Kapoor
A cross sectional study was conducted among adult patients (N=280) between 20-40 years at different stages of tuberculosis (TB) treatment. The data was collected from the different DOTS centres in Manipur, Northeast India. Several anthropometric measurements and the indices of adiposity like body mass index (BMI), waist hip ratio (WHR), waist height ratio (WHtR) and grand mean thickness (GMT) were studied. Wasting of muscle mass and decrease in fat percentage because of chronic disease (TB) and overall improvement in these components with antitubercular treatment was observed. This study suggests that body composition is a useful marker to indicate temporal recovery of patients undergoing tuberculosis treatment and determine treatment outcome.
{"title":"Outcome of Tuberculosis Treatment on Measures of Obesity and Adiposity Indices Among the Tribals of Northeast India","authors":"M. Tungdim, Heemanshu Aurora, S. Kapoor","doi":"10.4172/2161-1068.1000182","DOIUrl":"https://doi.org/10.4172/2161-1068.1000182","url":null,"abstract":"A cross sectional study was conducted among adult patients (N=280) between 20-40 years at different stages of tuberculosis (TB) treatment. The data was collected from the different DOTS centres in Manipur, Northeast India. Several anthropometric measurements and the indices of adiposity like body mass index (BMI), waist hip ratio (WHR), waist height ratio (WHtR) and grand mean thickness (GMT) were studied. Wasting of muscle mass and decrease in fat percentage because of chronic disease (TB) and overall improvement in these components with antitubercular treatment was observed. This study suggests that body composition is a useful marker to indicate temporal recovery of patients undergoing tuberculosis treatment and determine treatment outcome.","PeriodicalId":74235,"journal":{"name":"Mycobacterial diseases : tuberculosis & leprosy","volume":"5 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2015-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70581109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-05-20DOI: 10.4172/2161-1068.1000183
I. G. Sardella, Ana Carla de Paulo Mulinari, L. Fonseca, M. Saad
Mycobacterium tuberculosis-specific antigens (Ag) would be of important value in developing immunodiagnostic test for tuberculosis (TB), however human heterogeneous recognition of Ag epitopes may result in specificity variation of the test. In this work, we described the construction of two fusion proteins, based on two peptides from MPT-64 disrupting MT-10 Ag, F1-MT10.3 (1M-40S):CE15 (173G-187A):MT10.3 (41S-96) and F2- MT10.3 (1M-40S):MPT64 (91L-205A):MT10.3 (41S-96), and their potential immunoreactivity in TB sera. These fusion genes were cloned in expression vector, inserted in E. coli, and their proteins were expressed and purified. Using ELISA technique purified fused proteins and single full antigens were evaluated for IgA, IgM and IgG in sera from individuals diagnosed with pulmonary tuberculosis (TB) and controls with other pulmonary disease. The F1 construction generated a new peptide and F2 generate two modified peptides compared with the single full proteins. Testing of the tuberculosis human sera, the constructions were recognized by all antibodies types but the best results was obtained for ELISA-IgA which predominantly recognized the F2 (66.7%) and F1 (58.3%), follow by full single antigens MT10.3 (41.7%) and MPT64 (16.7%) keeping the highest specificity (95.5%), hitherto being unnoticed. Reactivity of IgG-F1 and IgM-F2 showed higher UAC than full MT10.2 and MPT64. The data demonstrated the viability of the constructions and the usefulness of molecule modification for obtains potential immune reactivity improvement, deserving further immunological characterization.
{"title":"Cloning, Expression and Characterization of Fusion Proteins Based on Peptides of Rv1980c Disrupting Rv3019c Sequence and Evaluation of its Potential Immunoreactivity in Pulmonary Tuberculosis Sera","authors":"I. G. Sardella, Ana Carla de Paulo Mulinari, L. Fonseca, M. Saad","doi":"10.4172/2161-1068.1000183","DOIUrl":"https://doi.org/10.4172/2161-1068.1000183","url":null,"abstract":"Mycobacterium tuberculosis-specific antigens (Ag) would be of important value in developing immunodiagnostic test for tuberculosis (TB), however human heterogeneous recognition of Ag epitopes may result in specificity variation of the test. In this work, we described the construction of two fusion proteins, based on two peptides from MPT-64 disrupting MT-10 Ag, F1-MT10.3 (1M-40S):CE15 (173G-187A):MT10.3 (41S-96) and F2- MT10.3 (1M-40S):MPT64 (91L-205A):MT10.3 (41S-96), and their potential immunoreactivity in TB sera. These fusion genes were cloned in expression vector, inserted in E. coli, and their proteins were expressed and purified. Using ELISA technique purified fused proteins and single full antigens were evaluated for IgA, IgM and IgG in sera from individuals diagnosed with pulmonary tuberculosis (TB) and controls with other pulmonary disease. The F1 construction generated a new peptide and F2 generate two modified peptides compared with the single full proteins. Testing of the tuberculosis human sera, the constructions were recognized by all antibodies types but the best results was obtained for ELISA-IgA which predominantly recognized the F2 (66.7%) and F1 (58.3%), follow by full single antigens MT10.3 (41.7%) and MPT64 (16.7%) keeping the highest specificity (95.5%), hitherto being unnoticed. Reactivity of IgG-F1 and IgM-F2 showed higher UAC than full MT10.2 and MPT64. The data demonstrated the viability of the constructions and the usefulness of molecule modification for obtains potential immune reactivity improvement, deserving further immunological characterization.","PeriodicalId":74235,"journal":{"name":"Mycobacterial diseases : tuberculosis & leprosy","volume":"5 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2015-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70581154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-05-02DOI: 10.4172/2161-1068.1000181
Billie Caceda, J. Garrós, U. Mj, M. Sada, M. Machó, R. Cisterna
We present a case of severe Mycobacterium kansasii infection due to the clinical and radiological progression and complicated with syndrome of inappropriate antidiuretic hormone secretion (SIADH). This 54-year-old male alcoholic and heavy smoker as predisposing factors presented severe anorexia, asthenia and cachexia. Abundant acid-fast bacilli (AFB) were observed in the patient’s sputum and pleural fluid after Ziehl-Neelsen (ZN) staining. The strain was identified as M. kansasii by culture. A treatment with isoniazid, pyrazinamide, ethambutol, clarithromycin and moxifloxacin were then initiated. The course of the illness includes impaired consciousness, behavioural disturbances and agitation; we believe was a consequence of SIADH, because of the severe hyponatremia with low serum osmolality condition. Also, generalized or local infections are important and unregarded causes of SIADH. Multiple infectious diseases are associated with this syndrome [1]. An awareness of this www.omicsonline.org/open-access/pathogenicity-of-entamoeba-species-depends-on-cell-line-conversiongenome-reprogramming-and-epigenetic-gene-regulation-2157-7013-1000245.phppathogen with clinical, radiologic, and microbiologic parameters are all needed to establish the diagnosis of this infection and the eradication of disease requires prolonged combination drug therapy.
{"title":"A Case of Severe Mycobacterium kansasii Infection Complicated with the Syndrome of Inappropriate Antidiuretic Hormone Secretion (SIADH)","authors":"Billie Caceda, J. Garrós, U. Mj, M. Sada, M. Machó, R. Cisterna","doi":"10.4172/2161-1068.1000181","DOIUrl":"https://doi.org/10.4172/2161-1068.1000181","url":null,"abstract":"We present a case of severe Mycobacterium kansasii infection due to the clinical and radiological progression and complicated with syndrome of inappropriate antidiuretic hormone secretion (SIADH). This 54-year-old male alcoholic and heavy smoker as predisposing factors presented severe anorexia, asthenia and cachexia. Abundant acid-fast bacilli (AFB) were observed in the patient’s sputum and pleural fluid after Ziehl-Neelsen (ZN) staining. The strain was identified as M. kansasii by culture. A treatment with isoniazid, pyrazinamide, ethambutol, clarithromycin and moxifloxacin were then initiated. The course of the illness includes impaired consciousness, behavioural disturbances and agitation; we believe was a consequence of SIADH, because of the severe hyponatremia with low serum osmolality condition. Also, generalized or local infections are important and unregarded causes of SIADH. Multiple infectious diseases are associated with this syndrome [1]. An awareness of this www.omicsonline.org/open-access/pathogenicity-of-entamoeba-species-depends-on-cell-line-conversiongenome-reprogramming-and-epigenetic-gene-regulation-2157-7013-1000245.phppathogen with clinical, radiologic, and microbiologic parameters are all needed to establish the diagnosis of this infection and the eradication of disease requires prolonged combination drug therapy.","PeriodicalId":74235,"journal":{"name":"Mycobacterial diseases : tuberculosis & leprosy","volume":"5 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2015-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70581381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-04-21DOI: 10.4172/2161-1068.1000180
D. Zingué
Mycobacterium tuberculosis complex and non-tuberculosis mycobacterium are still caused serious problems of public health around the world, mainly in resource limited settings. The emergence of drug resistant, failure and relapse of mycobacterium infections treatment made the need of efficient and cost-effective tools for treatment monitoring. In resource limited settings with poor facilities, the transfer and implementation by National Tuberculosis Programs of fluorescein diacetate vitality staining could be used as an alternative method of mycobacteria culture for drug therapy monitoring in district level. We described in this review the potential use of fluorescein diacetate staining in the monitoring of infectious disease caused by mycobacterium strains.
{"title":"Fluorescein Diacetate Staining- A Strategy of Laboratories Capacity Building for Efficient Management of Mycobacterium Infections Treatment in Resource Limited Settings","authors":"D. Zingué","doi":"10.4172/2161-1068.1000180","DOIUrl":"https://doi.org/10.4172/2161-1068.1000180","url":null,"abstract":"Mycobacterium tuberculosis complex and non-tuberculosis mycobacterium are still caused serious problems of public health around the world, mainly in resource limited settings. The emergence of drug resistant, failure and relapse of mycobacterium infections treatment made the need of efficient and cost-effective tools for treatment monitoring. In resource limited settings with poor facilities, the transfer and implementation by National Tuberculosis Programs of fluorescein diacetate vitality staining could be used as an alternative method of mycobacteria culture for drug therapy monitoring in district level. We described in this review the potential use of fluorescein diacetate staining in the monitoring of infectious disease caused by mycobacterium strains.","PeriodicalId":74235,"journal":{"name":"Mycobacterial diseases : tuberculosis & leprosy","volume":"5 1","pages":"1-3"},"PeriodicalIF":0.0,"publicationDate":"2015-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70580832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-01-01DOI: 10.4172/2161-1068.1000186
R. Khan, S. Quaiser, A. Viswanath
Even with the best of resources tuberculosis remains one of the leading causes of morbidity and mortality in the developing countries. With the 0 by 25 initiative taken up by the International Society of Nephrology, renal tuberculosis still remains an important, albeit ignored cause of acute kidney injury in low resource settings. With proper evaluation considerable morbidity can be minimised and the quality of life of patients can be improved upon.
即使拥有最好的资源，结核病仍然是发展中国家发病率和死亡率的主要原因之一。随着国际肾脏病学会(International Society of Nephrology)提出的“0 by 25”倡议，肾结核仍然是低资源环境下急性肾损伤的一个重要但被忽视的原因。通过适当的评估，可以将相当大的发病率降至最低，并改善患者的生活质量。
{"title":"Renal Tuberculosis: The Enigma Continues","authors":"R. Khan, S. Quaiser, A. Viswanath","doi":"10.4172/2161-1068.1000186","DOIUrl":"https://doi.org/10.4172/2161-1068.1000186","url":null,"abstract":"Even with the best of resources tuberculosis remains one of the leading causes of morbidity and mortality in the developing countries. With the 0 by 25 initiative taken up by the International Society of Nephrology, renal tuberculosis still remains an important, albeit ignored cause of acute kidney injury in low resource settings. With proper evaluation considerable morbidity can be minimised and the quality of life of patients can be improved upon.","PeriodicalId":74235,"journal":{"name":"Mycobacterial diseases : tuberculosis & leprosy","volume":"5 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70581584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-12-07DOI: 10.4172/2161-1068.1000176
M. Menéndez, M. Jiménez, J. Yubero, M. J. García
The novel slow-grower Mycobacterium kumamotonense was previously misidentified as Mycobacterium tuberculosis complex using commercial probes. Similarly to other slow-growers that cross-react with the tubercle bacilli using commercial probes, M. kumamotonense is carrying two copies of the ribosomal RNA operon (rrn) per genome. Analysis of the corresponding rrn regions allowed the identification of sequences putatively involved in that cross-reactivity.
{"title":"Mycobacterium kumamotonense, another Member of the Mycobacterium terrae Complex Unusually Carrying Two Copies of the Ribosomal RNA Operon","authors":"M. Menéndez, M. Jiménez, J. Yubero, M. J. García","doi":"10.4172/2161-1068.1000176","DOIUrl":"https://doi.org/10.4172/2161-1068.1000176","url":null,"abstract":"The novel slow-grower Mycobacterium kumamotonense was previously misidentified as Mycobacterium tuberculosis complex using commercial probes. Similarly to other slow-growers that cross-react with the tubercle bacilli using commercial probes, M. kumamotonense is carrying two copies of the ribosomal RNA operon (rrn) per genome. Analysis of the corresponding rrn regions allowed the identification of sequences putatively involved in that cross-reactivity.","PeriodicalId":74235,"journal":{"name":"Mycobacterial diseases : tuberculosis & leprosy","volume":"4 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2014-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70581103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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