Aim: To assess the chemo-immunomodulatory effects of doxorubicin-loaded cerium oxide nanoparticles coated with oleyl amine-linked cyclic RGDfK peptide (CeNP+Dox+RGD) to target both gliomas and its tumor microenvironment (TME) via integrin receptors. Materials & methods: CeNP+Dox+RGD nanoparticles are synthesized by the sequential addition of cerium III chloride heptahydrate, beta-cyclodextrin, oleic acid, and F127 micelle (CeNP). Doxorubicin was then loaded into CeNPs and coated with oleyl amine-linked cyclic RGDfK peptide to form stable CeNP+Dox+RGD nanoparticles. Results: CeNP+Dox+RGD nanoparticles crossed blood-brain barrier (BBB) effectively and demonstrated threefold enhanced survivability in glioma-bearing mice. The IHC profiling of glial tumor cross-sections showed increased CD80 expression (M1 TAMs) and decreased arginase-1 expression (M2 TAMs). Conclusion: CeNP+Dox+RGD can be an immunotherapeutic treatment option to combat glioblastoma.
{"title":"Integrin receptor-targeted, doxorubicin-loaded cerium oxide nanoparticles delivery to combat glioblastoma.","authors":"Gayathri Koula, Venu Yakati, Hari Krishnareddy Rachamalla, Keerti Bhamidipati, Muralidharan Kathirvel, Rajkumar Banerjee, Nagaprasad Puvvada","doi":"10.1080/17435889.2024.2350357","DOIUrl":"10.1080/17435889.2024.2350357","url":null,"abstract":"<p><p><b>Aim:</b> To assess the chemo-immunomodulatory effects of doxorubicin-loaded cerium oxide nanoparticles coated with oleyl amine-linked cyclic RGDfK peptide (CeNP+Dox+RGD) to target both gliomas and its tumor microenvironment (TME) via integrin receptors. <b>Materials & methods:</b> CeNP+Dox+RGD nanoparticles are synthesized by the sequential addition of cerium III chloride heptahydrate, beta-cyclodextrin, oleic acid, and F127 micelle (CeNP). Doxorubicin was then loaded into CeNPs and coated with oleyl amine-linked cyclic RGDfK peptide to form stable CeNP+Dox+RGD nanoparticles. <b>Results:</b> CeNP+Dox+RGD nanoparticles crossed blood-brain barrier (BBB) effectively and demonstrated threefold enhanced survivability in glioma-bearing mice. The IHC profiling of glial tumor cross-sections showed increased CD80 expression (M1 TAMs) and decreased arginase-1 expression (M2 TAMs). <b>Conclusion:</b> CeNP+Dox+RGD can be an immunotherapeutic treatment option to combat glioblastoma.</p>","PeriodicalId":74240,"journal":{"name":"Nanomedicine (London, England)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11318704/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141443883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-20Epub Date: 2024-06-26DOI: 10.1080/17435889.2024.2357530
Jennifer T Cruz, Karen Álvarez, Víctor H Orozco, Mauricio Rojas, Raul A Morales-Luckie, Luis F Giraldo
Aim: To investigate the effect of surfactant type on curcumin-loaded (CUR) PLGA nanoparticles (NPs) to modulate monocyte functions. Materials & methods: The nanoprecipitation method was used, and PLGA NPs were designed using Pluronic F127 (F127) and/or lecithin (LEC) as surfactants. Results: The Z-average of the NPs was <200 nm, they had a spherical shape, Derjaguin-Muller-Toporov modulus >0.128 MPa, they were stable during storage at 4°C, ζ-potential ∼-40 mV, polydispersity index <0.26 and % EE of CUR >94%. PLGA-LEC/F127 NPs showed favorable physicochemical and nanomechanical properties. These NPs were bound and internalized mainly by monocytes, suppressed monocyte-induced reactive oxygen species production, and decreased the ability of monocytes to modulate T-cell proliferation. Conclusion: These results demonstrate the potential of these NPs for targeted therapy.
{"title":"PLGA-LEC/F127 hybrid nanoparticles loaded with curcumin and their modulatory effect on monocytes.","authors":"Jennifer T Cruz, Karen Álvarez, Víctor H Orozco, Mauricio Rojas, Raul A Morales-Luckie, Luis F Giraldo","doi":"10.1080/17435889.2024.2357530","DOIUrl":"10.1080/17435889.2024.2357530","url":null,"abstract":"<p><p><b>Aim:</b> To investigate the effect of surfactant type on curcumin-loaded (CUR) PLGA nanoparticles (NPs) to modulate monocyte functions. <b>Materials & methods</b>: The nanoprecipitation method was used, and PLGA NPs were designed using Pluronic F127 (F127) and/or lecithin (LEC) as surfactants. <b>Results:</b> The Z-average of the NPs was <200 nm, they had a spherical shape, Derjaguin-Muller-Toporov modulus >0.128 MPa, they were stable during storage at 4°C, ζ-potential ∼-40 mV, polydispersity index <0.26 and % EE of CUR >94%. PLGA-LEC/F127 NPs showed favorable physicochemical and nanomechanical properties. These NPs were bound and internalized mainly by monocytes, suppressed monocyte-induced reactive oxygen species production, and decreased the ability of monocytes to modulate T-cell proliferation. <b>Conclusion:</b> These results demonstrate the potential of these NPs for targeted therapy.</p>","PeriodicalId":74240,"journal":{"name":"Nanomedicine (London, England)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11382718/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141452344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-20DOI: 10.1080/17435889.2024.2359364
Moaz A Eltabeeb, Menna M Abdellatif, Mohamed A El-Nabarawi, Mahmoud H Teaima, Mohammed I A Hamed, Khaled M Darwish, Mariam Hassan, Ahmed Me Hamdan, Raghda Rabe Hamed
Aim: Our investigation aims to estimate the antifungal effect of propranolol hydrochloride (PNL). Methods: Oleosomes (OLs) were fabricated by thin-film hydration and evaluated for entrapment efficiency (EE%), particle size (PS), polydispersity index (PDI), zeta potential (ZP), and amount of drug released after 6 h Q6h (%). Results: The optimal OL showed a rounded shape with optimum characteristics. The ex-vivo permeation and confocal laser scanning microscopy verified the prolonged release and well deposition of PNL-loaded OLs-gel. The in-silico assessment demonstrated the good stability of PNL with OLs' ingredients. In vivo evaluations for PNL-loaded OLs-gel showed a good antifungal impact against Candida albicans with good safety. Conclusion: This work highlights the potential of PNL-loaded OLs-gel as a potential treatment for candida vaginal infection.
目的:我们的调查旨在评估盐酸普萘洛尔(PNL)的抗真菌效果。方法:采用薄膜水合法制造油小体(OLs),并评估其抗真菌效果(单位:mg/g):通过薄膜水合法制备油小体(OLs),并评估其包载效率(EE%)、粒度(PS)、多分散指数(PDI)、Zeta 电位(ZP)以及 6 小时 Q6h 后的药物释放量(%)。结果显示最佳 OL 呈圆形,具有最佳特性。体内外渗透和共聚焦激光扫描显微镜验证了负载 PNL 的 OLs-凝胶的长期释放和良好沉积。室内评估表明,PNL 与 OL 成分具有良好的稳定性。载入 PNL 的 OLs-凝胶的体内评估显示,它对白色念珠菌具有良好的抗真菌效果,而且安全性良好。结论这项研究强调了 PNL 负载 OLs-凝胶作为治疗念珠菌阴道感染的潜在药物的潜力。
{"title":"Chitosan decorated oleosomes loaded propranolol hydrochloride hydrogel repurposed for <i>Candida albicans</i>-vaginal infection.","authors":"Moaz A Eltabeeb, Menna M Abdellatif, Mohamed A El-Nabarawi, Mahmoud H Teaima, Mohammed I A Hamed, Khaled M Darwish, Mariam Hassan, Ahmed Me Hamdan, Raghda Rabe Hamed","doi":"10.1080/17435889.2024.2359364","DOIUrl":"10.1080/17435889.2024.2359364","url":null,"abstract":"<p><p><b>Aim:</b> Our investigation aims to estimate the antifungal effect of propranolol hydrochloride (PNL). <b>Methods:</b> Oleosomes (OLs) were fabricated by thin-film hydration and evaluated for entrapment efficiency (EE%), particle size (PS), polydispersity index (PDI), zeta potential (ZP), and amount of drug released after 6 h Q6h (%). <b>Results:</b> The optimal OL showed a rounded shape with optimum characteristics. The <i>ex-vivo</i> permeation and confocal laser scanning microscopy verified the prolonged release and well deposition of PNL-loaded OLs-gel. The <i>in-silico</i> assessment demonstrated the good stability of PNL with OLs' ingredients. <i>In vivo</i> evaluations for PNL-loaded OLs-gel showed a good antifungal impact against <i>Candida albicans</i> with good safety. <b>Conclusion:</b> This work highlights the potential of PNL-loaded OLs-gel as a potential treatment for candida vaginal infection.</p>","PeriodicalId":74240,"journal":{"name":"Nanomedicine (London, England)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11318686/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141433534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-20DOI: 10.1080/17435889.2024.2354159
Pooja Kathait, Pradeep Kumar Patel, Alakh N Sahu
Exosomes, a category of extracellular vesicle (EV), are phospholipid bilayer structures ranging from 30 to 150 nm, produced by various organisms through the endosomal pathway. Recent studies have established the utilization of exosomes as nanocarriers for drug distribution across various therapeutic areas including cancer, acute liver injury, neuroprotection, oxidative stress, inflammation, etc. The importance of plant-derived exosomes and exosome vesicles derived from mammalian cells or milk, loaded with potent plant bioactives for various therapeutic indications are discussed along with insights into future perspectives. Moreover, this review provides a detailed understanding of exosome biogenesis, their composition, classification, stability of different types of exosomes, and different routes of administration along with the standard techniques used for isolating, purifying, and characterizing exosomes.
{"title":"Harnessing exosomes and plant-derived exosomes as nanocarriers for the efficient delivery of plant bioactives.","authors":"Pooja Kathait, Pradeep Kumar Patel, Alakh N Sahu","doi":"10.1080/17435889.2024.2354159","DOIUrl":"https://doi.org/10.1080/17435889.2024.2354159","url":null,"abstract":"<p><p>Exosomes, a category of extracellular vesicle (EV), are phospholipid bilayer structures ranging from 30 to 150 nm, produced by various organisms through the endosomal pathway. Recent studies have established the utilization of exosomes as nanocarriers for drug distribution across various therapeutic areas including cancer, acute liver injury, neuroprotection, oxidative stress, inflammation, etc. The importance of plant-derived exosomes and exosome vesicles derived from mammalian cells or milk, loaded with potent plant bioactives for various therapeutic indications are discussed along with insights into future perspectives. Moreover, this review provides a detailed understanding of exosome biogenesis, their composition, classification, stability of different types of exosomes, and different routes of administration along with the standard techniques used for isolating, purifying, and characterizing exosomes.</p>","PeriodicalId":74240,"journal":{"name":"Nanomedicine (London, England)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141433535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-01Epub Date: 2024-02-29DOI: 10.2217/nnm-2023-0282
Rita Castro, James H Adair, Andrea M Mastro, Thomas Neuberger, Gail L Matters
Vascular cell adhesion molecule-1 (VCAM-1) was identified over 2 decades ago as an endothelial adhesion receptor involved in leukocyte recruitment and cell-based immune responses. In atherosclerosis, a chronic inflammatory disease of the blood vessels that is the leading cause of death in the USA, endothelial VCAM-1 is robustly expressed beginning in the early stages of the disease. The interactions of circulating immune cells with VCAM-1 on the activated endothelial cell surface promote the uptake of monocytes and the progression of atherosclerotic lesions in susceptible vessels. Herein, we review the role of VCAM-1 in atherosclerosis and the use of VCAM-1 binding peptides, antibodies and aptamers as targeting agents for nanoplatforms for early detection and treatment of atherosclerotic disease.
{"title":"VCAM-1-targeted nanoparticles to diagnose, monitor and treat atherosclerosis.","authors":"Rita Castro, James H Adair, Andrea M Mastro, Thomas Neuberger, Gail L Matters","doi":"10.2217/nnm-2023-0282","DOIUrl":"10.2217/nnm-2023-0282","url":null,"abstract":"<p><p>Vascular cell adhesion molecule-1 (VCAM-1) was identified over 2 decades ago as an endothelial adhesion receptor involved in leukocyte recruitment and cell-based immune responses. In atherosclerosis, a chronic inflammatory disease of the blood vessels that is the leading cause of death in the USA, endothelial VCAM-1 is robustly expressed beginning in the early stages of the disease. The interactions of circulating immune cells with VCAM-1 on the activated endothelial cell surface promote the uptake of monocytes and the progression of atherosclerotic lesions in susceptible vessels. Herein, we review the role of VCAM-1 in atherosclerosis and the use of VCAM-1 binding peptides, antibodies and aptamers as targeting agents for nanoplatforms for early detection and treatment of atherosclerotic disease.</p>","PeriodicalId":74240,"journal":{"name":"Nanomedicine (London, England)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139992064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-01Epub Date: 2024-03-01DOI: 10.2217/nnm-2023-0292
Anam Rais, Shubham Sharma, Prashant Mishra, Luqman Ahmad Khan, Tulika Prasad
Aim: The development of carbon quantum dots (C-QDs) as nanotrackers to understand drug-pathogen interactions, virulence and multidrug resistance. Methods: Microwave synthesis of C-QDs was performed using citric acid and polyethylene glycol. Further, in vitro toxicity was evaluated and imaging applications were demonstrated in Candida albicans isolates. Results: Well-dispersed, ultra small C-QDs exhibited no cyto/microbial/reactive oxygen species-mediated toxicity and internalized effectively in Candida yeast and hyphal cells. C-QDs were employed for confocal imaging of drug-sensitive and -resistant cells, and a study of the yeast-to-hyphal transition using atomic force microscopy in Candida was conducted for the first time. Conclusion: These biocompatible C-QDs have promising potential as next-generation nanotrackers for in vitro and in vivo targeted cellular and live imaging, after functionalization with biomolecules and drugs.
{"title":"Biocompatible carbon quantum dots as versatile imaging nanotrackers of fungal pathogen - <i>Candida albicans</i>.","authors":"Anam Rais, Shubham Sharma, Prashant Mishra, Luqman Ahmad Khan, Tulika Prasad","doi":"10.2217/nnm-2023-0292","DOIUrl":"10.2217/nnm-2023-0292","url":null,"abstract":"<p><p><b>Aim:</b> The development of carbon quantum dots (C-QDs) as nanotrackers to understand drug-pathogen interactions, virulence and multidrug resistance. <b>Methods:</b> Microwave synthesis of C-QDs was performed using citric acid and polyethylene glycol. Further, <i>in vitro</i> toxicity was evaluated and imaging applications were demonstrated in <i>Candida albicans</i> isolates. <b>Results:</b> Well-dispersed, ultra small C-QDs exhibited no cyto/microbial/reactive oxygen species-mediated toxicity and internalized effectively in <i>Candida</i> yeast and hyphal cells. C-QDs were employed for confocal imaging of drug-sensitive and -resistant cells, and a study of the yeast-to-hyphal transition using atomic force microscopy in <i>Candida</i> was conducted for the first time. <b>Conclusion:</b> These biocompatible C-QDs have promising potential as next-generation nanotrackers for <i>in vitro</i> and <i>in vivo</i> targeted cellular and live imaging, after functionalization with biomolecules and drugs.</p>","PeriodicalId":74240,"journal":{"name":"Nanomedicine (London, England)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139998518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-01Epub Date: 2024-02-29DOI: 10.2217/nnm-2024-0029
Alakh N Sahu, Debadatta Mohapatra, Pratap Chandra Acharya
Tweetable abstract Invasomes and invasomal gel are ultraflexible, soft vesicular, phospholipid based nanocarriers with deeper skin penetration ability for transdermal applications of drugs and phytopharmaceuticals.
Tweetable 摘要 Invasomes 和 invasomal gel 是一种超柔韧、软泡状、磷脂基纳米载体,具有更深的皮肤渗透能力,可用于药物和植物药的透皮应用。
{"title":"Nanovesicular ultraflexible invasomes and invasomal gel for transdermal delivery of phytopharmaceuticals.","authors":"Alakh N Sahu, Debadatta Mohapatra, Pratap Chandra Acharya","doi":"10.2217/nnm-2024-0029","DOIUrl":"10.2217/nnm-2024-0029","url":null,"abstract":"<p><p>Tweetable abstract Invasomes and invasomal gel are ultraflexible, soft vesicular, phospholipid based nanocarriers with deeper skin penetration ability for transdermal applications of drugs and phytopharmaceuticals.</p>","PeriodicalId":74240,"journal":{"name":"Nanomedicine (London, England)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139992063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-01Epub Date: 2024-02-23DOI: 10.2217/nnm-2023-0353
Jie Mao, Gi-Deok Eom, Keon-Woong Yoon, Min-Ju Kim, Ki-Back Chu, Hae-Ji Kang, Fu-Shi Quan
Aims: To develop an effective universal vaccine against antigenically different influenza viruses. Materials & methods: We generated influenza virus-like particles (VLPs) expressing the H1 and H3 antigens with or without M2e5x. VLP-induced immune responses and crossprotection against H1N1, H3N2 or H5N1 viruses were assessed to evaluate their protective efficacy. Results: H1H3M2e5x immunization elicited higher crossreactive IgG antibodies than H1H3 VLPs. Upon challenge, both VLPs enhanced lung IgG, IgA and germinal center B-cell responses compared with control. While these VLPs conferred protection, H1H3M2e5x showed greater lung viral load reduction than H1H3 VLPs with minimal body weight loss. Conclusion: Utilizing VLPs containing dual-hemagglutinin, along with M2e5x, can be a vaccination strategy for inducing crossprotection against influenza A viruses.
{"title":"Crossprotection induced by virus-like particles containing influenza dual-hemagglutinin and M2 ectodomain.","authors":"Jie Mao, Gi-Deok Eom, Keon-Woong Yoon, Min-Ju Kim, Ki-Back Chu, Hae-Ji Kang, Fu-Shi Quan","doi":"10.2217/nnm-2023-0353","DOIUrl":"10.2217/nnm-2023-0353","url":null,"abstract":"<p><p><b>Aims:</b> To develop an effective universal vaccine against antigenically different influenza viruses. <b>Materials & methods:</b> We generated influenza virus-like particles (VLPs) expressing the H1 and H3 antigens with or without M2e5x. VLP-induced immune responses and crossprotection against H1N1, H3N2 or H5N1 viruses were assessed to evaluate their protective efficacy. <b>Results:</b> H1H3M2e5x immunization elicited higher crossreactive IgG antibodies than H1H3 VLPs. Upon challenge, both VLPs enhanced lung IgG, IgA and germinal center B-cell responses compared with control. While these VLPs conferred protection, H1H3M2e5x showed greater lung viral load reduction than H1H3 VLPs with minimal body weight loss. <b>Conclusion:</b> Utilizing VLPs containing dual-hemagglutinin, along with M2e5x, can be a vaccination strategy for inducing crossprotection against influenza A viruses.</p>","PeriodicalId":74240,"journal":{"name":"Nanomedicine (London, England)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139934575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-01Epub Date: 2024-02-09DOI: 10.2217/nnm-2023-0318
Sobia Noreen, Fahad Pervaiz, Muhammad Ijaz, Muhammad Farhan Hanif, Jam Riyan Hamza, Hassan Mahmood, Hina Shoukat, Irsah Maqbool, Muhammad Azeem Ashraf
Aim: This study aimed to develop and evaluate pH-sensitive docetaxel-loaded thiolated hyaluronic acid (HA-SH) nanoparticles (NPs) for targeted treatment of colon cancer. Materials & methods: HA-SH, synthesized via oxidation and subsequent covalent linkage to cysteamine, served as the precursor for developing HA-SH NPs through polyelectrolyte complexation involving chitosan and thiol-bearing HA. Results & conclusion: HA-SH NPs displayed favorable characteristics, with small particle sizes (184-270 nm), positive zeta potential (15.4-18.6 mV) and high entrapment efficiency (91.66-95.02%). In vitro, NPs demonstrated potent mucoadhesion and enhanced cytotoxicity compared with free docetaxel. In vivo assessments confirmed safety and biocompatibility, suggesting HA-SH NPs as promising pH-sensitive drug carriers with enhanced antitumor activity for colorectal cancer treatments.
{"title":"pH-sensitive docetaxel-loaded chitosan/thiolated hyaluronic acid polymeric nanoparticles for colorectal cancer.","authors":"Sobia Noreen, Fahad Pervaiz, Muhammad Ijaz, Muhammad Farhan Hanif, Jam Riyan Hamza, Hassan Mahmood, Hina Shoukat, Irsah Maqbool, Muhammad Azeem Ashraf","doi":"10.2217/nnm-2023-0318","DOIUrl":"10.2217/nnm-2023-0318","url":null,"abstract":"<p><p><b>Aim:</b> This study aimed to develop and evaluate pH-sensitive docetaxel-loaded thiolated hyaluronic acid (HA-SH) nanoparticles (NPs) for targeted treatment of colon cancer. <b>Materials & methods:</b> HA-SH, synthesized via oxidation and subsequent covalent linkage to cysteamine, served as the precursor for developing HA-SH NPs through polyelectrolyte complexation involving chitosan and thiol-bearing HA. <b>Results & conclusion:</b> HA-SH NPs displayed favorable characteristics, with small particle sizes (184-270 nm), positive zeta potential (15.4-18.6 mV) and high entrapment efficiency (91.66-95.02%). <i>In vitro</i>, NPs demonstrated potent mucoadhesion and enhanced cytotoxicity compared with free docetaxel. <i>In vivo</i> assessments confirmed safety and biocompatibility, suggesting HA-SH NPs as promising pH-sensitive drug carriers with enhanced antitumor activity for colorectal cancer treatments.</p>","PeriodicalId":74240,"journal":{"name":"Nanomedicine (London, England)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139708761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: The effectiveness of a drug is dependent on its accumulation at the site of therapeutic action, as well as its time in circulation. The aim of the research was the creation of stable albumin/tannin (punicalagin, punicalin) particles, which might serve for the delivery of medicines. Methods: Numerous chromatographic and analytical methods, docking analyses and in vivo testing were applied and used. Results: Stable tannin-albumin/medicine particles with a diameter of ∼100 nm were obtained. The results of in vivo experiments proved that tannin-albumin particles are more stable than albumin particles. Conclusion: Based on the experiments and docking analyses, these stable particles can carry an extended number of medicines, with diverse chemical structures.
{"title":"Tannin-albumin particles as stable carriers of medicines.","authors":"Nelli Ohanyan, Narek Abelyan, Arpi Manukyan, Vardan Hayrapetyan, Samvel Chailyan, Susanna Tiratsuyan, Kristine Danielyan","doi":"10.2217/nnm-2023-0275","DOIUrl":"10.2217/nnm-2023-0275","url":null,"abstract":"<p><p><b>Background:</b> The effectiveness of a drug is dependent on its accumulation at the site of therapeutic action, as well as its time in circulation. The aim of the research was the creation of stable albumin/tannin (punicalagin, punicalin) particles, which might serve for the delivery of medicines. <b>Methods:</b> Numerous chromatographic and analytical methods, docking analyses and <i>in vivo</i> testing were applied and used. <b>Results:</b> Stable tannin-albumin/medicine particles with a diameter of ∼100 nm were obtained. The results of <i>in vivo</i> experiments proved that tannin-albumin particles are more stable than albumin particles. <b>Conclusion:</b> Based on the experiments and docking analyses, these stable particles can carry an extended number of medicines, with diverse chemical structures.</p>","PeriodicalId":74240,"journal":{"name":"Nanomedicine (London, England)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139725222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}