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The presence of native semi-fossorial herbivores enhances the coupling between species and functional diversity in alpine meadows 原生半穴居食草动物的存在增强了高寒草甸物种与功能多样性之间的耦合
IF 6.4 1区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2026-04-15 Epub Date: 2026-01-14 DOI: 10.1016/j.agee.2026.110235
Yuan Yuan Duan, Kun Qin, Yu Chen, Jie Li, Ni Wang, Xiao Dan Sun, Yan Ping Bai, Lin Han Li, Zheng Gang Guo
Restoring biodiversity and ecosystem functionality in degraded alpine grasslands is central to reversing ecological degradation and maintaining ecosystem services. However, despite the increasing recognition of functional diversity as a key driver of ecosystem function, the role of native herbivores in shaping such diversity remains poorly understood. This study evaluated the ecological consequences of the presence of plateau pikas (Ochotona curzoniae) in alpine meadows by comparing plant species diversity, functional diversity, and their relationships with soil properties between presence and absence plots. The results showed that the presence of plateau pikas significantly enhanced plant species richness and functional trait diversity (FAD2, MFAD, FDc), while reducing Pielou’s index and functional divergence (FDiv). It also strengthened the coupling between species and functional species, suggesting enhanced ecological functionality through increased trait complementarity. Plant diversity was primarily driven by soil total potassium and phosphorus in absence plots, whereas soil organic carbon and nitrogen to phosphorus ratio were positively associated with plant diversity, soil total nitrogen, phosphorus, and carbon to phosphorus ratio showed negative associations in presence plots. This indicates that the presence of plateau pikas decouples plant diversity from its original soil nutrient controls, shifting community assembly toward activity-driven processes. These findings highlight plateau pika as a native ecosystem engineer that facilitates biodiversity recovery and functional integration, even as it disrupts traditional soil-vegetation feedbacks. The study emphasizes the need to consider moderate native herbivore activity as a sustainable grassland restoration strategy to enhance biodiversity, ecosystem functionality, and resilience under environmental change.
恢复退化高寒草原的生物多样性和生态系统功能是扭转生态退化和维持生态系统服务的关键。然而,尽管越来越多的人认识到功能多样性是生态系统功能的关键驱动因素,但本地食草动物在形成这种多样性中的作用仍然知之甚少。本研究通过比较高原鼠兔(Ochotona curzoniae)在高寒草甸存在的植物物种多样性、功能多样性及其与土壤性质的关系,评价了高原鼠兔(Ochotona curzoniae)存在的生态后果。结果表明,高原鼠兔的存在显著提高了植物物种丰富度和功能性状多样性(FAD2、MFAD、FDc),降低了Pielou指数和功能分化度(FDiv)。它还加强了物种与功能物种之间的耦合,表明通过增加性状互补性来增强生态功能。土壤有机碳和氮磷比与植物多样性呈显著正相关,土壤全氮、全磷和碳磷比与植物多样性呈显著负相关。这表明高原鼠兔的存在将植物多样性从其原始土壤养分控制中解耦,将群落组装转向活动驱动过程。这些发现突出了高原鼠兔作为原生生态系统工程师,促进生物多样性恢复和功能整合,即使它破坏了传统的土壤-植被反馈。该研究强调,有必要考虑适度的本地食草动物活动作为可持续的草地恢复策略,以增强环境变化下的生物多样性、生态系统功能和恢复力。
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引用次数: 0
Effects of no-tillage, mulching, drip irrigation, and nitrogen fertilization on greenhouse gas emissions, soil carbon sequestration, and crop yields in dryland agroecosystems: A meta-analysis 免耕、覆盖、滴灌和氮肥对旱地农业生态系统温室气体排放、土壤固碳和作物产量的影响:meta分析
IF 6.4 1区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2026-04-15 Epub Date: 2026-01-14 DOI: 10.1016/j.agee.2026.110242
Rahmatullah Hashimi , Girisha K. Ganjegunte , Saurav Kumar , Santosh S. Palmate , Jhaman Das Suthar
Dryland agriculture contributes substantially to greenhouse gas (GHG) emissions, primarily due to flood irrigation, excessive nitrogen fertilization, and intensive soil disturbance. However, the influence of climate-smart agriculture (CSA) practices in mitigating GHG emissions under dry conditions remains inconsistent. This meta-analysis was conducted by reviewing 87 peer-reviewed papers to assess the impact of CSA practices, such as no-tillage (NT), drip irrigation (DI), plastic mulching (PSM), straw mulching (STM), and nitrogen fertilization (NFY), on soil organic carbon (SOC) content, global warming potential (GWP), GHG intensity (GHGI), and crop yields in arid and semi-arid agroecosystems. DI was the most effective single practice, reducing CO2, N2O, GWP, and GHGI by 9.8 %, 54.7 %, 9.5 %, and 10.6 %, respectively. Compared to conventional tillage (CT), NT with straw retention (NTS) significantly increased SOC content by 14.8 % and wheat yield by 5.2 %, while long-term (>5 years) NT reduced GWP and GHGI by 14.2 % and 14.1 %, respectively. Conversely, STM and high NFY rate increased GWP by 27.7 % and 41.5 %, respectively. Although the high NFY rate increased overall crop yield by 70.6 %, indicating at a substantial environmental cost. In contrast, a low NFY rate reduced GHGI by 42.6 %, suggesting a viable mitigation pathway. Overall, these findings underscore a fundamental trade-off between yield and emissions, indicating that integrating precise nutrient management, drip irrigation, and no-tillage with optimized residue retention can provide a synergistic strategy to enhance productivity while simultaneously mitigating GHG emissions in dryland agroecosystems.
旱地农业对温室气体(GHG)排放有很大贡献,主要是由于洪水灌溉、过度施氮和严重的土壤干扰。然而,气候智慧型农业(CSA)实践在减少干旱条件下温室气体排放方面的影响仍然不一致。通过对87篇同行评议论文的荟萃分析,评估了免耕(NT)、滴灌(DI)、地膜(PSM)、秸秆覆盖(STM)和氮肥(NFY)等CSA措施对干旱和半干旱农业生态系统土壤有机碳(SOC)含量、全球变暖潜势(GWP)、温室气体强度(GHGI)和作物产量的影响。DI是最有效的单一做法,分别减少了9.8% %、54.7% %、9.5% %和10.6 %的CO2、N2O、GWP和GHGI。与常规耕作(CT)相比,秸秆还田(NTS)显著提高了土壤有机碳含量(14.8 %)和小麦产量(5.2 %),而长期(>;5年)土壤还田使GWP和GHGI分别降低了14.2 %和14.1 %。相反,STM和高NFY率分别使GWP增加27.7% %和41.5 %。尽管高净产量使作物总产量提高了70.6% %,但这表明付出了巨大的环境代价。相比之下,低NFY率使温室气体排放量减少了42.6% %,这表明存在可行的缓解途径。总的来说,这些发现强调了产量和排放之间的基本权衡,表明将精确的养分管理、滴灌和免耕与优化的残留物保留相结合,可以提供一种协同策略,在提高生产力的同时减少旱地农业生态系统的温室气体排放。
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引用次数: 0
Soil microbial community structure and carbon dynamics in response to compost and livestock management in grassland soils 草地土壤微生物群落结构和碳动态对堆肥和牲畜管理的响应
IF 6.4 1区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2026-04-15 Epub Date: 2026-01-23 DOI: 10.1016/j.agee.2026.110261
Laura L. de Sosa , María José Carpio , Marta Gil-Martínez , Mathieu Thevenot , Olivier Mathieu , Engracia Madejón , Marco Panettieri
Organic amendments are widely promoted to restore soil fertility in degraded agroecosystems, yet their effects may depend strongly on land-use history. We evaluated the interactive influence of composted olive mill waste (alperujo) and three grazing management regimes—livestock exclusion, ovine, and equine grazing—on soil chemical properties, microbial communities, and carbon (C) mineralization in a Mediterranean dehesa. Across treatments, livestock exclusion consistently increased soil C and N contents compared with grazed areas, with total C (TC) 42 % higher than in equine-grazed plots and 52 % higher than in ovine-grazed plots, and total nitrogen (TN) 18 % higher than in equine plots (with similar TN levels in ovine plots across samplings). Compost addition further increased plant-available phosphorus (P) and potassium (K) by 243 % and 270 % in the more degraded equine-grazed soils. Compost did not significantly increase TC or TN but induced marked shifts in bacterial and fungal community composition, with responses modulated by grazing legacy and initial fertility. Taxonomic changes were most pronounced within oligotrophic and copiotrophic bacterial groups (e.g., Chloroflexi, Verrucomicrobiota) and certain fungal phyla (e.g., Rozellomycota, Ascomycota), although overall microbial diversity remained largely unchanged. Functionally, compost reduced both initial C mineralization rates and cumulative respiration, as well as the priming effect, especially in exclusion and ovine soils. This suppression likely reflects the compost’s high chemical stability, low labile C content, and its influence on microbial carbon-use strategies. Spatial heterogeneity of organic inputs remained a challenge in grazed systems, but compost partially mitigated nutrient depletion in under-enriched areas. Our findings highlight that the effects of organic amendments are context-dependent, shaped by grazing legacy and nutrient status. They underscore the importance of integrating organic amendments with tailored grazing management to optimize nutrient balance, microbial function, and long-term soil C sequestration in Mediterranean rangelands. However, as this study was conducted at a single site with one compost type over a short-term period, further research across sites, compost types, and longer time scales is needed to generalize these findings
在退化的农业生态系统中,有机改良剂被广泛推广以恢复土壤肥力,但其效果可能在很大程度上取决于土地利用历史。我们评估了堆肥橄榄磨坊废弃物(alperujo)和三种放牧管理制度(牲畜排除、羊和马放牧)对地中海地区土壤化学性质、微生物群落和碳(C)矿化的相互作用影响。在不同处理中,与放牧区相比,排除牲畜持续增加了土壤C和N含量,总C (TC)比放牧区高42 %,比放牧区高52 %,总氮(TN)比放牧区高18 %(不同采样中,绵羊地的TN水平相似)。在退化程度较高的马放牧土壤中,添加堆肥进一步提高了植物速效磷(P)和钾(K),分别提高了243 %和270 %。堆肥没有显著增加土壤总氮和总碳,但引起了细菌和真菌群落组成的显著变化,这种变化受放牧遗产和初始肥力的调节。尽管总体微生物多样性基本保持不变,但在低营养和共营养细菌群(如氯氟菌群、疣菌群)和某些真菌门(如罗泽菌群、子囊菌群)中分类学变化最为明显。在功能上,堆肥降低了初始碳矿化速率和累积呼吸速率,并降低了启动效应,特别是在排异土和羊土中。这种抑制可能反映了堆肥的高化学稳定性,低不稳定C含量及其对微生物碳利用策略的影响。在放牧系统中,有机投入的空间异质性仍然是一个挑战,但堆肥在一定程度上缓解了营养不足地区的养分消耗。我们的研究结果强调,有机修正的影响是环境依赖的,由放牧遗产和营养状况决定。他们强调了将有机改良与量身定制的放牧管理相结合的重要性,以优化地中海牧场的营养平衡、微生物功能和长期土壤碳封存。然而,由于本研究是在短期内使用一种堆肥类型的单一地点进行的,因此需要进一步研究跨地点、堆肥类型和更长的时间尺度来推广这些发现
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引用次数: 0
Long-term fertilization shaped soil organic matter molecular diversity via microbial functional regulation in paddy soil 长期施肥通过微生物功能调控影响水稻土有机质分子多样性
IF 6.4 1区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2026-04-15 Epub Date: 2026-01-23 DOI: 10.1016/j.agee.2026.110266
Shuotong Chen , Keyao Zhu , Fang Gao , Bowen Yang , Xianlin Ke , Wenhai Mi
The molecular diversity of soil organic matter (SOM) underpins its persistence, yet how fertilization alters the microbial assembly of SOM at the molecular level remains poorly understood in paddy soils. Here, we combined a 14-year field experiment in subtropical China with pyrolysis gas chromatography/mass spectrometry (Py-GC/MS) and metagenomics to elucidate how fertilization regimes shape SOM molecular diversity across soil depths (topsoil, 0–15 cm; subsoil, 15–30 cm). Four fertilization regimes were compared: no fertilization (CK), synthetic NPK fertilizers alone (NPK), and NPK combined with either cattle manure (NPKM) or rice straw (NPKS). Organic amendments enhanced soil organic matter (SOM) molecular diversity in a depth-specific manner. Manure application increased SOM richness, Shannon index, and evenness in topsoil by 35.9 %, 15.4 %, and 9.6 %, respectively, whereas straw addition increased subsoil richness and Shannon index by 30.6 % and 16.4 % relative to CK (p < 0.05). In contrast, β-diversity was governed more by soil depth (R² = 0.32, p < 0.001) than fertilization (R² = 0.19, p < 0.001). These patterns were underpinned by distinct shifts in microbial functional traits. Straw enriched hemicellulose-degrading CAZymes, whereas manure stimulated lignin-oxidizing and chitin-degrading enzymes and upregulated lipid biosynthesis genes, resulting in enhanced aromatic turnover and lipid accumulation in topsoil. Subsoil lipid pools declined with downregulation of sterol and glycerophospholipid genes. Network and path analyses demonstrated that microbial functional traits, rather than fertilization inputs alone, were the dominant mediators linking fertilization to SOM molecular diversification (path coefficient = 0.94, p < 0.05). Overall, our findings highlight that organic amendments expand microbial metabolic repertoires and strengthen SOM–microbe interactions, thereby enhancing SOM molecular complexity and carbon stabilization. This mechanistic understanding provides a microbiome-informed framework for optimizing fertilization strategies to sustain soil carbon and ecosystem functioning in paddy systems.
土壤有机质(SOM)的分子多样性是其持续存在的基础,但在水稻土中,施肥如何在分子水平上改变SOM的微生物组合仍然知之甚少。在这里,我们结合了在中国亚热带地区进行的14年的田间实验,结合热解气相色谱/质谱(Py-GC/MS)和宏基因组学来阐明施肥制度如何影响土壤深度(表层土壤,0-15 cm;底土,15-30 cm)的SOM分子多样性。比较了4种施肥方案:不施肥(CK)、单独施用氮磷钾(NPK)和氮磷钾与牛粪(NPKM)或稻草(NPKS)混合施用。有机改良剂对土壤有机质(SOM)分子多样性具有深度特异性。施用有机肥使表层土壤有机质丰富度、Shannon指数和均匀度比对照分别提高了35.9 %、15.4 %和9.6 %,而秸秆使底土丰富度和Shannon指数分别提高了30.6 %和16.4 % (p <; 0.05)。相比之下,β-多样性受土壤深度(R²= 0.32,p <; 0.001)的影响大于施肥(R²= 0.19,p <; 0.001)。这些模式是由微生物功能特征的明显变化所支撑的。秸秆富集了半纤维素降解酶,而粪肥则刺激了木质素氧化酶和几丁质降解酶,并上调了脂质生物合成基因,导致表层土壤芳香转化和脂质积累增加。随着甾醇和甘油磷脂基因的下调,底土脂库减少。网络和通径分析表明,微生物功能性状,而不是单独的施肥投入,是连接施肥与SOM分子多样化的主要媒介(通径系数= 0.94,p <; 0.05)。总的来说,我们的研究结果强调,有机修饰扩大了微生物代谢谱,加强了SOM与微生物的相互作用,从而提高了SOM分子的复杂性和碳的稳定性。这种机制的理解为优化施肥策略提供了一个微生物信息框架,以维持水稻系统的土壤碳和生态系统功能。
{"title":"Long-term fertilization shaped soil organic matter molecular diversity via microbial functional regulation in paddy soil","authors":"Shuotong Chen ,&nbsp;Keyao Zhu ,&nbsp;Fang Gao ,&nbsp;Bowen Yang ,&nbsp;Xianlin Ke ,&nbsp;Wenhai Mi","doi":"10.1016/j.agee.2026.110266","DOIUrl":"10.1016/j.agee.2026.110266","url":null,"abstract":"<div><div>The molecular diversity of soil organic matter (SOM) underpins its persistence, yet how fertilization alters the microbial assembly of SOM at the molecular level remains poorly understood in paddy soils. Here, we combined a 14-year field experiment in subtropical China with pyrolysis gas chromatography/mass spectrometry (Py-GC/MS) and metagenomics to elucidate how fertilization regimes shape SOM molecular diversity across soil depths (topsoil, 0–15 cm; subsoil, 15–30 cm). Four fertilization regimes were compared: no fertilization (CK), synthetic NPK fertilizers alone (NPK), and NPK combined with either cattle manure (NPKM) or rice straw (NPKS). Organic amendments enhanced soil organic matter (SOM) molecular diversity in a depth-specific manner. Manure application increased SOM richness, Shannon index, and evenness in topsoil by 35.9 %, 15.4 %, and 9.6 %, respectively, whereas straw addition increased subsoil richness and Shannon index by 30.6 % and 16.4 % relative to CK (<em>p</em> &lt; 0.05). In contrast, β-diversity was governed more by soil depth (R² = 0.32, <em>p</em> &lt; 0.001) than fertilization (R² = 0.19, <em>p</em> &lt; 0.001). These patterns were underpinned by distinct shifts in microbial functional traits. Straw enriched hemicellulose-degrading CAZymes, whereas manure stimulated lignin-oxidizing and chitin-degrading enzymes and upregulated lipid biosynthesis genes, resulting in enhanced aromatic turnover and lipid accumulation in topsoil. Subsoil lipid pools declined with downregulation of sterol and glycerophospholipid genes. Network and path analyses demonstrated that microbial functional traits, rather than fertilization inputs alone, were the dominant mediators linking fertilization to SOM molecular diversification (path coefficient = 0.94, <em>p</em> &lt; 0.05). Overall, our findings highlight that organic amendments expand microbial metabolic repertoires and strengthen SOM–microbe interactions, thereby enhancing SOM molecular complexity and carbon stabilization. This mechanistic understanding provides a microbiome-informed framework for optimizing fertilization strategies to sustain soil carbon and ecosystem functioning in paddy systems.</div></div>","PeriodicalId":7512,"journal":{"name":"Agriculture, Ecosystems & Environment","volume":"400 ","pages":"Article 110266"},"PeriodicalIF":6.4,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146033913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Organic fertilization and hayseed application in traditional hay meadows: A pathway to biodiversity and ecological sustainability 传统草甸有机施肥与草籽施用:生物多样性与生态可持续性的途径
IF 6.4 1区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2026-04-15 Epub Date: 2026-01-21 DOI: 10.1016/j.agee.2026.110214
Monika Janišová , Polina Dayneko , Martin Magnes , Dariia Borovyk , Lubov Borsukevych , Denys Vynokurov , Katarína Devánová , Anna Kuzemko , Corina Iosif , Anamaria Iuga , Iveta Škodová
In Central Europe, low-intensity and low-input agriculture has fostered biodiverse landscapes and species-rich semi-natural grasslands, but implementation and ecological context of historical land-use practices remain poorly understood. We investigated meadow management practices that enhance hay productivity and quality in Poienile de sub Munte, Romania, a village with well-maintained historical land-use patterns. Specifically, we examined: (i) the implementation of manuring, ash fertilization, and hayseed application; (ii) their effects on plant species composition and diversity; and (iii) their potential in modern grassland conservation and restoration. Our study analyzed 47 vegetation plots, 30 hayseed samples, and land-use data from 30 farmers. Manure was mainly applied on gentle slopes, whereas ash was used in steep and hard-to-access areas. Hayseed was applied in 47 % of one-cut meadows and 33 % of two-cut meadows. Vegetation composition was primarily influenced by mowing frequency, soil calcium content, and the frequency of manuring and ash fertilization. Manuring decreased species richness by seven species through competitive exclusion favoring nitrogen-demanding species. In contrast, ash fertilization promoted oligotrophic specialists, including rare and vulnerable species, while increasing species richness by approximately seven species. Regular hayseed application enhanced richness through propagule supply without significant effect on vegetation composition. Hayseed composition differed from standing vegetation and was influenced by mowing time, with first-cut hay containing significantly more species and marginally more seeds than second-cut hay. Hayseed application creates a resilient system through continuous local seed reintroduction, effectively buffering historical disturbances and management variations. While this homogenizes vegetation composition across landscapes, it simultaneously maintains local diversity through enhancing functional connectivity, further supported by manuring and grazing. Traditional practices offer low-cost, accessible tools for restoring degraded grasslands, particularly when combining ash fertilization with locally-sourced hayseed under appropriate grazing regimes.
在中欧,低强度和低投入的农业培育了生物多样性景观和物种丰富的半自然草地,但对历史土地利用实践的实施和生态背景仍知之甚少。我们在罗马尼亚的Poienile de sub Munte村调查了提高干草生产力和质量的草甸管理措施,这是一个保持良好历史土地利用模式的村庄。具体来说,我们检查了:(i)实施施肥,灰施肥和干草施用;(ii)它们对植物物种组成和多样性的影响;(三)在现代草地保护与恢复中的潜力。我们的研究分析了47个植被样地、30个干草样本和30个农民的土地利用数据。肥料主要用于平缓的斜坡,而灰则用于陡峭和难以接近的地区。在47% %的一次刈割草地和33% %的两次刈割草地上施用草籽。植被组成主要受刈割频率、土壤钙含量、施肥和灰施肥频率的影响。施肥通过竞争排斥减少了7种物种的丰富度,有利于需氮物种。相比之下,灰施肥促进了贫营养专家,包括稀有和脆弱物种,同时增加了大约7种物种的丰富度。定期施用草籽可通过繁殖体供应增加丰富度,但对植被组成无显著影响。干草组成与立地不同,受刈割时间的影响,第一次刈割的干草比第二次刈割的干草含有更多的物种和略多的种子。Hayseed应用通过持续的本地种子重新引入创建了一个有弹性的系统,有效地缓冲了历史干扰和管理变化。虽然这使整个景观的植被组成同质化,但它同时通过增强功能连通性来保持当地的多样性,并进一步得到施肥和放牧的支持。传统做法为恢复退化的草原提供了低成本、容易获得的工具,特别是在适当的放牧制度下,将灰施肥与当地来源的干草结合起来。
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引用次数: 0
In situ 15N tracer quantification of fertilizer- and soil-derived NH3, NO, and N2O emissions in maize fields 玉米田肥料和土壤源性NH3、NO和N2O排放的15N示踪剂原位定量研究
IF 6.4 1区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2026-04-15 Epub Date: 2026-01-06 DOI: 10.1016/j.agee.2025.110202
Zhi Quan , Geshere Abdisa Gurmesa , Bin Huang , Xue Li , Chenxia Su , Yanzhi Wang , Xin Chen , Yunting Fang
Agricultural soils are major sources of reactive nitrogen (N) gases, yet distinguishing fertilizer-derived emissions from those originating in the soil remains a key challenge for accurate N management. We conducted an in situ 15N tracing experiment in a maize field in Northeast China using 15N-labeled urea (49.7 % 15N, 200 kg N ha⁻¹), integrating passive adsorption and static chamber techniques to quantify source-specific emissions of ammonia (NH3), nitric oxide (NO), and nitrous oxide (N2O). The results revealed distinct timing in the peak emissions of these N gases. NH3 emission peaked first (6.4 kg N ha⁻¹ cumulative loss) and was mainly driven by soil ammonium levels whereas subsequent NO (3.8 kg N ha⁻¹) and N2O (1.4 kg N ha⁻¹) peaks were primarily regulated by temperature and soil nitrate availability. The synchronous bimodal 15N dynamics of NO and N₂O indicated coupled nitrification-denitrification processes, with higher 15N enrichment in NO (mean 20 %) than in N2O (11 %), suggesting stronger nitrification control on NO production. Fertilizer-derived N accounted for 67 %, 52 %, and 30 % of total NH3, NO, and N2O emissions, respectively. However, fertilizer-induced soil N transformations via priming and legacy effects led to underestimation of the total influence of fertilizer in 15N tracing. These findings challenge conventional emission factor models, which may overlook indirect N emissions from agricultural inputs, and highlight the need to incorporate soil N priming and legacy dynamics into agricultural N footprint assessments.
农业土壤是活性氮(N)气体的主要来源,然而区分来自土壤的肥料排放仍然是准确氮管理的关键挑战。我们在中国东北的玉米田进行了15N原位示踪实验,使用15N标记的尿素(49.7 % 15N, 200 kg N ha⁻¹),结合被动吸附和静态室技术来量化氨(NH3)、一氧化氮(NO)和氧化亚氮(N2O)的源特异性排放。结果显示,这些N气体的峰值排放时间明显不同。NH3排放量首先达到峰值(6.4 kg N ha⁻¹累积损失),主要受土壤铵含量的驱动,而随后的NO(3.8 kg N ha⁻¹)和N2O(1.4 kg N ha⁻¹)峰值主要受温度和土壤硝酸盐可用性的调节。NO和N₂O的同步双峰15N动力学表明了耦合的硝化-反硝化过程,NO中的15N富集(平均20 %)高于N2O(11 %),表明硝化对NO的产生有更强的控制作用。肥料衍生的N分别占NH3、NO和N2O总排放量的67 %、52 %和30 %。然而,通过启动效应和遗留效应引起的肥料诱导土壤氮转化导致低估了肥料在15N示踪中的总影响。这些发现挑战了传统的排放因子模型,这些模型可能忽略了农业投入的间接氮排放,并强调了将土壤氮激发和遗留动态纳入农业氮足迹评估的必要性。
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引用次数: 0
Natural habitats enhance pollination services in apple and sweet cherry orchards: Evidence from Southern Mediterranean agroecosystems 自然栖息地增强了苹果和甜樱桃园的授粉服务:来自南地中海农业生态系统的证据
IF 6.4 1区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2026-04-15 Epub Date: 2026-01-20 DOI: 10.1016/j.agee.2026.110220
Camila B. García , Keira Dymond , Manuel López-Aliste , Pablo Díaz-Siefer , Francisco E. Fontúrbel , Michael P.D. Garratt , Juan L. Celis-Diez
Wild pollinators play a critical role in crop production. However, the ongoing loss of natural habitats threatens pollination services and food security. We evaluated how the percentage of surrounding natural habitat and the distance from orchard edges influence wild floral visitors and pollination deficit in sweet cherry and apple orchards in Mediterranean Chile. Additionally, we examined the relationship between floral visitation and fruit production. We studied eighteen orchards of each crop and classified them into three categories according to the percentage of natural habitats within a 1 km radius: < 35 %, 36–70 % and > 70 %. Three pollination treatments (supplementary, open, and exclusion) were applied at the edge and into the orchard interior. We quantified floral visitor richness, floral visits, fruit set and fruit quality. Our results showed that sweet cherry orchards with less than 35 % surrounding natural habitat had significantly lower wild insect species richness and floral visits compared to orchards with > 35 %. In both crops, species richness and wild floral visits declined with increasing distance from the orchard edge. Wild floral visitors enhanced fruit set in both crops, while honey bees contributed to sweet cherry fruit set and apple seed set. Although insect pollination improved fruit quality, we found no direct relationship between flower visitation and fruit quality. In apple orchards with > 70 % natural habitat, fruit weight deficit was lower at the edge compared to both the interior and the edges of orchards with < 35 % of natural habitat. We recommend conserving natural habitats around orchards to support wild pollinator diversity and visitation, thereby reducing pollination deficits.
野生传粉媒介在作物生产中起着至关重要的作用。然而,自然栖息地的持续丧失威胁着授粉服务和粮食安全。我们评估了智利地中海地区甜樱桃和苹果园周围自然栖息地的百分比和距离果园边缘的距离如何影响野生花卉游客和授粉赤字。此外,我们还研究了开花与果实产量之间的关系。我们研究了每种作物的18个果园,并根据1 km半径内自然栖息地的百分比将其分为三类:<; 35 %,36-70 %和>; 70 %。在果园边缘和果园内部分别进行了补充授粉、开放授粉和隔离授粉三种授粉处理。我们量化了访花丰富度、访花次数、坐果量和果实质量。结果表明,与环绕自然生境35% %以下的果园相比,环绕自然生境35% %以下的甜樱桃果园野生昆虫种类丰富度和访花次数显著低于环绕自然生境35% %的果园。随着离果园边缘距离的增加,两种作物的物种丰富度和野生花卉到访量均呈下降趋势。野生花卉游客促进了这两种作物的坐果,而蜜蜂则促进了甜樱桃坐果和苹果坐果。虽然昆虫授粉改善了果实品质,但我们发现访花与果实品质之间没有直接关系。在自然生境为>; 70 %的苹果园中,果实重亏在边缘处比在自然生境为<; 35 %的苹果园的内部和边缘处都要低。我们建议保护果园周围的自然栖息地,以支持野生传粉者的多样性和来访,从而减少授粉缺陷。
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引用次数: 0
Irrigation-driven shifts in weed community composition, functional identity, and functional diversity: Evidence from a Mediterranean vineyard 灌溉驱动的杂草群落组成、功能特性和功能多样性的变化:来自地中海葡萄园的证据
IF 6.4 1区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2026-04-15 Epub Date: 2026-01-15 DOI: 10.1016/j.agee.2026.110244
J. García-Guerra, J. Dorado, J.M. Peña
This study examines how different irrigation levels influence weed community composition and functional structure in a Mediterranean vineyard, explicitly considering spatial heterogeneity (vineyard rows vs. inter-rows) and seasonal variability (spring vs. summer). Weed surveys and functional trait measurements (vegetative plant height, leaf area, specific leaf area, and leaf dry matter content) were conducted under three irrigation treatments (low: Kc = 0.2; moderate: Kc = 0.4; high: Kc = 0.8). Irrigation significantly modified weed community composition, particularly during the active irrigation period in summer, with effects persisting into the following spring. Increased irrigation consistently boosted total weed cover and favoured competitor species, while stress-tolerant species declined under higher irrigation levels. Functionally, greater water availability promoted resource-acquisitive traits (e.g. higher leaf area and specific leaf area, lower leaf dry matter content). Dominant species, such as Cirsium arvense, exhibited pronounced intraspecific trait plasticity, notably increasing leaf area under high irrigation, underscoring the critical role of trait plasticity in shaping community responses. Functional diversity metrics—functional richness and divergence—increased significantly under high irrigation, particularly within vineyard rows directly receiving water. Moreover, irrigation influenced weed communities beyond directly irrigated areas and periods, demonstrating spatial and temporal legacy effects. Agronomically, our findings demonstrate that increased irrigation levels may intensify weed pressure, highlighting the necessity for irrigation management strategies to incorporate ecological insights into weed community dynamics to achieve an optimal balance between productivity and ecological sustainability.
本研究考察了不同灌溉水平对地中海葡萄园杂草群落组成和功能结构的影响,明确考虑了空间异质性(葡萄园行与行间)和季节变异(春季与夏季)。在低Kc = 0.2、中Kc = 0.4、高Kc = 0.8 3种灌溉处理下进行了杂草调查和功能性状测量(营养株高、叶面积、比叶面积和叶干物质含量)。灌溉显著改变了杂草群落组成,特别是在夏季的积极灌溉期,这种影响将持续到次年春季。增加灌溉持续增加杂草覆盖总量和有利的竞争物种,而耐胁迫物种在高灌溉水平下下降。在功能上,较高的水分有效性促进了资源获取性状(如较高的叶面积和比叶面积,较低的叶干物质含量)。优势种如卷叶草(Cirsium arvense)表现出明显的种内性状可塑性,在高灌溉条件下叶面积显著增加,说明性状可塑性在形成群落响应中的关键作用。功能多样性指标(功能丰富度和多样性)在高灌溉条件下显著增加,特别是在直接接受水的葡萄园行内。此外,灌溉对杂草群落的影响超出了直接灌区和灌期,表现出时空遗留效应。在农艺学上,我们的研究结果表明,灌溉水平的增加可能会加剧杂草的压力,强调灌溉管理策略的必要性,将生态学见解纳入杂草群落动态,以实现生产力和生态可持续性之间的最佳平衡。
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引用次数: 0
Livestock production systems shape alpha and beta diversity of small mammal assemblages 畜牧业生产系统塑造了小型哺乳动物群落的α和β多样性
IF 6.4 1区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2026-04-15 Epub Date: 2026-01-09 DOI: 10.1016/j.agee.2025.110176
Rodrigo Javier Alonso , Rosario Lovera , Melanie Ruiz , Malena Rospide , Martin Neyen Lammen , Daniela Paula Montes de Oca , Juliana Sánchez , Regino Cavia
Within highly modified environments, livestock production systems shape small mammal assemblages, which are particularly relevant due to their responsiveness to local habitat structure and management practices, and their role as agricultural pests and reservoirs of zoonotic pathogens. This study examines changes in the composition and structure of small mammal assemblages, assessed through taxonomic alpha and beta diversity, across different livestock farming systems, seasons, years, and geographical regions in northeast Buenos Aires province, Argentina. Seasonal sampling was conducted on dairy farms, intensive and extensive pig farms, and feedlots from 2008 to 2021, though not continuously. For alpha and beta diversity comparisons, we used abundance-based diversity indices and multivariate ordination analyses, respectively. A total of 1317 individuals from ten species were captured, with Rattus norvegicus (n = 777) as the dominant species. Multivariate analyses revealed that the composition of small mammal assemblages was influenced by the type of farm and season. Species richness was highest on dairy and intensive pig farms, whereas feedlots exhibited greater evenness with more common and dominant species. Seasonal variations affected diversity patterns, with the highest species richness in fall and winter. The results highlight how different types of farms, seasons and landscape contexts shape small mammal communities, changing abiotic factors and introducing interactions between native and invasive species. These findings underscore the need for tailored small mammal management strategies to mitigate biodiversity loss and zoonotic risks in livestock systems.
在高度改变的环境中,牲畜生产系统塑造了小型哺乳动物的群落,由于它们对当地栖息地结构和管理做法的反应,以及它们作为农业害虫和人畜共患病原体储存库的作用,这些群落尤为重要。本研究通过分类α和β多样性评估了阿根廷布宜诺斯艾利斯省东北部不同畜牧业系统、季节、年份和地理区域的小型哺乳动物组合的组成和结构变化。从2008年到2021年,对奶牛场、集约化和粗放化养猪场和饲养场进行了季节性抽样,但不是连续抽样。对于α和β多样性的比较,我们分别使用了基于丰度的多样性指数和多元排序分析。共捕获褐家鼠10种1317只,褐家鼠( = 777)为优势种。多因素分析表明,小型哺乳动物群落的组成受养殖场类型和季节的影响。物种丰富度在奶牛场和集约化养猪场最高,而饲养场表现出更大的均匀性,常见和优势物种较多。季节变化影响多样性格局,秋季和冬季物种丰富度最高。研究结果强调了不同类型的农场、季节和景观环境如何塑造小型哺乳动物群落,改变非生物因素,并引入本地和入侵物种之间的相互作用。这些发现强调需要制定量身定制的小型哺乳动物管理战略,以减轻牲畜系统中的生物多样性丧失和人畜共患病风险。
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引用次数: 0
Synergistic agricultural systems improve soil health and support sustainable land use in sandy soils 协同农业系统可改善土壤健康并支持沙质土壤的可持续土地利用
IF 6.4 1区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2026-04-15 Epub Date: 2026-01-07 DOI: 10.1016/j.agee.2025.110193
Camila P. Cagna , Moges K. Biru , Diego A.H.S. Leitao , Patricia A.B. Barreto-Garcia , Viviany Viriato , Jose C.B. Dubeux Jr. , Cassio A. Tormena , Marcio R. Nunes
Sandy soils cover around 900 million hectares globally and are increasingly targeted for agricultural expansion and food production. However, these soils are highly vulnerable to degradation due to their low nutrient and water retention capacity, poor structure, and susceptibility to compaction. Our objective was to identify conservation-based production systems to sustain or enhance the health of sandy soils. Soil physical, chemical, and biological properties and processes were assessed across three soil depths (0–5, 15–20, and 25–30 cm). Diversified cropping systems, particularly the sod-based rotation system combined with cover crops and nitrogen fertilization (SBR-GL34), improved overall soil health. This production system increased total soil porosity by up to 18 %, reduced subsurface bulk density by 11 %, and enhanced soil organic carbon stock by up to 18 % compared with business-as-usual (BAU). Labile fractions also improved, with permanganate-oxidizable carbon increasing by 38 %, while β-glucosidase and arylsulfatase increased by 60 % and 64 %, respectively. Despite these improvements, compared to native, all production systems had lower carbon stocks (55–65 %) and showed signes of soil physical degradation. Integrating cover crops, nitrogen fertilization, and grazing also enhanced microbial activity and nutrient cycling, mitigating the negative effects of converting from native to agricultural systems. Among all agricultural systems, SBR-GL34 showed the highest potential to improve (compared to BAU) and sustain (compared to native) soil health and productivity of sandy soils. Overall, combining appropriate nutrient management with long-term agricultural practices that reduce soil disturbance and maximize diversity and soil cover with live plants and crop residue supports sustainable agricultural production in sandy soils.
沙质土壤覆盖全球约9亿公顷,越来越多地成为农业扩张和粮食生产的目标。然而,这些土壤由于营养和保水能力低、结构差、易压实而极易退化。我们的目标是确定以保护为基础的生产系统,以维持或增强沙质土壤的健康。在三个土壤深度(0-5、15-20和25-30 cm)中评估了土壤的物理、化学和生物特性和过程。多样化的种植制度,特别是与覆盖作物和氮肥相结合的草基轮作制度(SBR-GL34),改善了土壤的整体健康状况。与常规生产(BAU)相比,该生产系统将土壤总孔隙度提高了18% %,将地下体积密度降低了11% %,并将土壤有机碳储量提高了18% %。活性组分也得到了改善,高锰酸盐可氧化碳增加了38 %,β-葡萄糖苷酶和芳基硫酸盐酶分别增加了60 %和64 %。尽管有这些改进,但与本地相比,所有生产系统的碳储量都较低(55 - 65% %),并显示出土壤物理退化的迹象。覆盖作物、氮肥和放牧的结合也增强了微生物活动和养分循环,减轻了从原生系统向农业系统转变的负面影响。在所有农业系统中,SBR-GL34在改善(与BAU相比)和维持(与原生相比)沙土土壤健康和生产力方面表现出最大的潜力。总体而言,将适当的养分管理与长期农业实践相结合,减少土壤干扰,最大限度地提高活植物和作物秸秆的多样性和土壤覆盖,可支持沙质土壤的可持续农业生产。
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