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Soil microbial community structure and carbon dynamics in response to compost and livestock management in grassland soils 草地土壤微生物群落结构和碳动态对堆肥和牲畜管理的响应
IF 6.4 1区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2026-01-23 DOI: 10.1016/j.agee.2026.110261
Laura L. de Sosa , María José Carpio , Marta Gil-Martínez , Mathieu Thevenot , Olivier Mathieu , Engracia Madejón , Marco Panettieri
Organic amendments are widely promoted to restore soil fertility in degraded agroecosystems, yet their effects may depend strongly on land-use history. We evaluated the interactive influence of composted olive mill waste (alperujo) and three grazing management regimes—livestock exclusion, ovine, and equine grazing—on soil chemical properties, microbial communities, and carbon (C) mineralization in a Mediterranean dehesa. Across treatments, livestock exclusion consistently increased soil C and N contents compared with grazed areas, with total C (TC) 42 % higher than in equine-grazed plots and 52 % higher than in ovine-grazed plots, and total nitrogen (TN) 18 % higher than in equine plots (with similar TN levels in ovine plots across samplings). Compost addition further increased plant-available phosphorus (P) and potassium (K) by 243 % and 270 % in the more degraded equine-grazed soils. Compost did not significantly increase TC or TN but induced marked shifts in bacterial and fungal community composition, with responses modulated by grazing legacy and initial fertility. Taxonomic changes were most pronounced within oligotrophic and copiotrophic bacterial groups (e.g., Chloroflexi, Verrucomicrobiota) and certain fungal phyla (e.g., Rozellomycota, Ascomycota), although overall microbial diversity remained largely unchanged. Functionally, compost reduced both initial C mineralization rates and cumulative respiration, as well as the priming effect, especially in exclusion and ovine soils. This suppression likely reflects the compost’s high chemical stability, low labile C content, and its influence on microbial carbon-use strategies. Spatial heterogeneity of organic inputs remained a challenge in grazed systems, but compost partially mitigated nutrient depletion in under-enriched areas. Our findings highlight that the effects of organic amendments are context-dependent, shaped by grazing legacy and nutrient status. They underscore the importance of integrating organic amendments with tailored grazing management to optimize nutrient balance, microbial function, and long-term soil C sequestration in Mediterranean rangelands. However, as this study was conducted at a single site with one compost type over a short-term period, further research across sites, compost types, and longer time scales is needed to generalize these findings
在退化的农业生态系统中,有机改良剂被广泛推广以恢复土壤肥力,但其效果可能在很大程度上取决于土地利用历史。我们评估了堆肥橄榄磨坊废弃物(alperujo)和三种放牧管理制度(牲畜排除、羊和马放牧)对地中海地区土壤化学性质、微生物群落和碳(C)矿化的相互作用影响。在不同处理中,与放牧区相比,排除牲畜持续增加了土壤C和N含量,总C (TC)比放牧区高42 %,比放牧区高52 %,总氮(TN)比放牧区高18 %(不同采样中,绵羊地的TN水平相似)。在退化程度较高的马放牧土壤中,添加堆肥进一步提高了植物速效磷(P)和钾(K),分别提高了243 %和270 %。堆肥没有显著增加土壤总氮和总碳,但引起了细菌和真菌群落组成的显著变化,这种变化受放牧遗产和初始肥力的调节。尽管总体微生物多样性基本保持不变,但在低营养和共营养细菌群(如氯氟菌群、疣菌群)和某些真菌门(如罗泽菌群、子囊菌群)中分类学变化最为明显。在功能上,堆肥降低了初始碳矿化速率和累积呼吸速率,并降低了启动效应,特别是在排异土和羊土中。这种抑制可能反映了堆肥的高化学稳定性,低不稳定C含量及其对微生物碳利用策略的影响。在放牧系统中,有机投入的空间异质性仍然是一个挑战,但堆肥在一定程度上缓解了营养不足地区的养分消耗。我们的研究结果强调,有机修正的影响是环境依赖的,由放牧遗产和营养状况决定。他们强调了将有机改良与量身定制的放牧管理相结合的重要性,以优化地中海牧场的营养平衡、微生物功能和长期土壤碳封存。然而,由于本研究是在短期内使用一种堆肥类型的单一地点进行的,因此需要进一步研究跨地点、堆肥类型和更长的时间尺度来推广这些发现
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引用次数: 0
Long-term fertilization shaped soil organic matter molecular diversity via microbial functional regulation in paddy soil 长期施肥通过微生物功能调控影响水稻土有机质分子多样性
IF 6.4 1区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2026-01-23 DOI: 10.1016/j.agee.2026.110266
Shuotong Chen , Keyao Zhu , Fang Gao , Bowen Yang , Xianlin Ke , Wenhai Mi
The molecular diversity of soil organic matter (SOM) underpins its persistence, yet how fertilization alters the microbial assembly of SOM at the molecular level remains poorly understood in paddy soils. Here, we combined a 14-year field experiment in subtropical China with pyrolysis gas chromatography/mass spectrometry (Py-GC/MS) and metagenomics to elucidate how fertilization regimes shape SOM molecular diversity across soil depths (topsoil, 0–15 cm; subsoil, 15–30 cm). Four fertilization regimes were compared: no fertilization (CK), synthetic NPK fertilizers alone (NPK), and NPK combined with either cattle manure (NPKM) or rice straw (NPKS). Organic amendments enhanced soil organic matter (SOM) molecular diversity in a depth-specific manner. Manure application increased SOM richness, Shannon index, and evenness in topsoil by 35.9 %, 15.4 %, and 9.6 %, respectively, whereas straw addition increased subsoil richness and Shannon index by 30.6 % and 16.4 % relative to CK (p < 0.05). In contrast, β-diversity was governed more by soil depth (R² = 0.32, p < 0.001) than fertilization (R² = 0.19, p < 0.001). These patterns were underpinned by distinct shifts in microbial functional traits. Straw enriched hemicellulose-degrading CAZymes, whereas manure stimulated lignin-oxidizing and chitin-degrading enzymes and upregulated lipid biosynthesis genes, resulting in enhanced aromatic turnover and lipid accumulation in topsoil. Subsoil lipid pools declined with downregulation of sterol and glycerophospholipid genes. Network and path analyses demonstrated that microbial functional traits, rather than fertilization inputs alone, were the dominant mediators linking fertilization to SOM molecular diversification (path coefficient = 0.94, p < 0.05). Overall, our findings highlight that organic amendments expand microbial metabolic repertoires and strengthen SOM–microbe interactions, thereby enhancing SOM molecular complexity and carbon stabilization. This mechanistic understanding provides a microbiome-informed framework for optimizing fertilization strategies to sustain soil carbon and ecosystem functioning in paddy systems.
土壤有机质(SOM)的分子多样性是其持续存在的基础,但在水稻土中,施肥如何在分子水平上改变SOM的微生物组合仍然知之甚少。在这里,我们结合了在中国亚热带地区进行的14年的田间实验,结合热解气相色谱/质谱(Py-GC/MS)和宏基因组学来阐明施肥制度如何影响土壤深度(表层土壤,0-15 cm;底土,15-30 cm)的SOM分子多样性。比较了4种施肥方案:不施肥(CK)、单独施用氮磷钾(NPK)和氮磷钾与牛粪(NPKM)或稻草(NPKS)混合施用。有机改良剂对土壤有机质(SOM)分子多样性具有深度特异性。施用有机肥使表层土壤有机质丰富度、Shannon指数和均匀度比对照分别提高了35.9 %、15.4 %和9.6 %,而秸秆使底土丰富度和Shannon指数分别提高了30.6 %和16.4 % (p <; 0.05)。相比之下,β-多样性受土壤深度(R²= 0.32,p <; 0.001)的影响大于施肥(R²= 0.19,p <; 0.001)。这些模式是由微生物功能特征的明显变化所支撑的。秸秆富集了半纤维素降解酶,而粪肥则刺激了木质素氧化酶和几丁质降解酶,并上调了脂质生物合成基因,导致表层土壤芳香转化和脂质积累增加。随着甾醇和甘油磷脂基因的下调,底土脂库减少。网络和通径分析表明,微生物功能性状,而不是单独的施肥投入,是连接施肥与SOM分子多样化的主要媒介(通径系数= 0.94,p <; 0.05)。总的来说,我们的研究结果强调,有机修饰扩大了微生物代谢谱,加强了SOM与微生物的相互作用,从而提高了SOM分子的复杂性和碳的稳定性。这种机制的理解为优化施肥策略提供了一个微生物信息框架,以维持水稻系统的土壤碳和生态系统功能。
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引用次数: 0
Multi-year drought has persistent forage quality and quantity effects that can be intensified by heavy grazing in semiarid rangelands 多年干旱具有持续的牧草质量和数量效应,在半干旱的草地上,重度放牧会加剧这种效应
IF 6.4 1区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2026-01-23 DOI: 10.1016/j.agee.2026.110231
Lauren M. Porensky , Sally E. Koerner , Amanda R. Williams , Megan L. Van Emon , Kevin R. Wilcox , Kimberly J. Komatsu , John D. Dietrich , Kurt O. Reinhart
Growing season droughts have major impacts on grassland vegetation and are predicted to become increasingly frequent in semiarid rangelands, but little is known about how droughts and post-drought legacies interact with grazing management to affect forage quality and quantity. In two semiarid prairie rangelands, we assessed the separate and combined effects of experimental rainfall reductions and grazing management strategies on ruminant forage quality and quantity over five years. We measured forage fiber content, organic matter digestibility, relative feed value, forage quantity, and the quantity of digestible forage. During a 2-year experimental rainfall reduction period, rainfall reductions decreased both forage quality and quantity at one site. At the second site, reductions were only apparent in heavily grazed plots. In the first year after experimental rainfall reduction treatments ended, plots that formerly received large rainfall reductions displayed strong legacy effects, with 26–57 % less digestible forage biomass but greater forage quality than controls. Increases in forage quality with former rainfall reduction treatments were strongest in plots that had received heavier grazing during the experimental drought period. Experimental treatments did not induce long-term changes in forage quantity, but rainfall reductions caused forage quality reductions that persisted up to three years after droughts ended. In contrast, grazing treatments did not have long-term effects on forage quality. Our results highlight that forage production capacity in North American grasslands is resilient to both drought stress and grazing disturbance, but these drivers can have additive and long-term effects on forage nutritive value. Heavy grazing during drought may strengthen both negative (during drought) and positive (post-drought) effects of drought on ungulate nutrition.
生长季干旱对草地植被有重大影响,预计将在半干旱牧场变得越来越频繁,但关于干旱和干旱后遗产如何与放牧管理相互作用,影响饲料质量和数量,我们知之甚少。在2个半干旱草原放牧区,研究了5年试验降水减少和放牧管理策略对反刍动物饲料质量和数量的单独和联合影响。测定了饲料纤维含量、有机质消化率、相对饲料价值、饲料量和可消化饲料量。在2年的试验减雨期内,一个试验点的牧草质量和数量均下降。在第二个地点,减少只在大量放牧的地块上明显。在试验减雨处理结束后的第一年,以前大量减雨的地块表现出强烈的遗留效应,可消化的饲料生物量比对照低26-57 %,但饲料质量比对照高。在试验干旱期放牧较重的地块,前减雨处理对牧草质量的提高最大。试验处理没有引起饲料数量的长期变化,但降雨减少导致饲料质量下降,这种下降持续到干旱结束后的三年。相比之下,放牧处理对牧草质量没有长期影响。我们的研究结果表明,北美草原的牧草生产能力对干旱胁迫和放牧干扰都具有弹性,但这些驱动因素可能对牧草营养价值具有附加性和长期影响。干旱期间的大量放牧可能会加强干旱对有蹄类动物营养的消极(干旱期间)和积极(干旱后)影响。
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引用次数: 0
Managed fly pollinators ensure berry crop yields under variable weather conditions 有管理的苍蝇传粉者确保在多变的天气条件下浆果作物的产量
IF 6.4 1区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2026-01-22 DOI: 10.1016/j.agee.2026.110254
Abby E. Davis , Raylea Rowbottom , Lena A. Schmidt , Jelena Preradovic , Karen C.B.S. Santos , Blake M. Dawson , Mavi Manrique , Derek Wright , Bar Shermeister , Brad Hocking , Joao Branco , Maurizio Rocchetti , Cameron Spurr , Romina Rader
Insect pollination is essential for global fruit and vegetable production, yet rising climate variability and widespread declines in bee populations threaten the reliability of this service. Flies (Diptera), despite their abundance and tolerance to different environmental conditions, remain largely overlooked as managed pollinators in agriculture. Here, we present field-based evidence from commercial farms in Australia showing that certain fly species can deliver effective and climate-resilient pollination services to high-value berry cropping systems. In replicated cage trials, European hover flies (Eristalis tenax) produced fruit comparable in weight and marketability to those pollinated by European honey bees (Apis mellifera) and Australian native stingless bees (Tetragonula carbonaria), whereas brown blow flies (Calliphora stygia) showed more variable performance. When scaled to full-sized commercial polytunnels, hover flies consistently produced market-quality blackberry fruit, matching open (mainly bee) pollination in spring and outperforming open pollination under the cooler, more variable conditions of winter. Hover fly-pollinated fruit equaled or exceeded the quality of hand-pollinated flowers and consistently outperformed self-pollinated controls. Across spring and winter floral visitation field surveys, hover flies maintained higher flower visitation than honey bees across cooler temperatures, broader humidity ranges, and early tomidday time periods, indicating greater climatic tolerance under field conditions. Together, these findings demonstrate that the hover fly, E. tenax, can provide scalable, climate-resilient pollination services and highlight the value of integrating flies into diversified pollination strategies to support resilient crop production under a changing climate.
昆虫授粉对全球水果和蔬菜生产至关重要,但气候变化的加剧和蜜蜂种群的广泛减少威胁着这一服务的可靠性。蝇类(双翅目)尽管数量丰富且对不同环境条件具有耐受性,但在农业中作为有管理的传粉媒介在很大程度上仍被忽视。在这里,我们提出了来自澳大利亚商业农场的实地证据,表明某些苍蝇物种可以为高价值浆果种植系统提供有效和气候适应性的授粉服务。在重复的笼子试验中,欧洲悬飞蝇(Eristalis tenax)结出的果实在重量和适销性上与欧洲蜜蜂(Apis mellifera)和澳大利亚本土无刺蜜蜂(Tetragonula carbonaria)授粉的果实相当,而褐飞蝇(Calliphora stygia)表现出更大的差异。当扩大到全尺寸的商业多通道时,盘旋蝇始终生产市场质量的黑莓果实,在春季与开放式(主要是蜜蜂)授粉相匹配,在冬季更凉爽,更多变的条件下优于开放式授粉。悬停蝇授粉的水果等于或超过了手授粉的花的质量,并始终优于自花授粉的对照。在春季和冬季的访花现场调查中,悬停蝇在较低的温度、较宽的湿度范围和午后时段比蜜蜂保持更高的访花量,表明在田间条件下具有更大的气候耐受性。总之,这些研究结果表明,悬飞蝇(E. tenax)可以提供可扩展的、气候适应性强的授粉服务,并突出了将苍蝇纳入多样化授粉策略的价值,以支持气候变化下的弹性作物生产。
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引用次数: 0
Pesticide-free fields under minimum tillage and flower strips enhance carabid beetles and spiders through increased overwintering and spill over processes 少耕免农药的田地和花带通过增加越冬和溢出过程来增强瓢虫和蜘蛛
IF 6.4 1区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2026-01-22 DOI: 10.1016/j.agee.2026.110250
Paul Bannwart , Antoine Gardarin , Violaine Deytieux , Stéphane Cordeau , Sandrine Petit
Enhancing generalist arthropod predators in arable farming can contribute to pest control and pesticide use reduction, and be achieved through a diversity of management options at field and farm scales. Most studies however link management options to densities of circulating adults, which gives little insight into potential source-sinks dynamics and the occurrence of ‘source’ habitats enabling overwintering. In this study, we assessed the assemblages of emerging and circulating ground-dwelling carabids and spiders during four months in a continuous mosaic of pesticide-free winter-sown crops under contrasted tillage regimes (minimum vs. conventional tillage) and sown flower strips bordering fields. We detected clear patterns, with high in-field carabid and spider overwintering densities than in adjacent flower strips, which were rarely a preferred overwintering habitat. Our results nevertheless indicate that within both taxa, different species exhibit different responses and source-sink dynamics. Our results also demonstrate the key role of pesticide-free fields under minimum tillage, acting both as a high-quality overwintering site for some dominant carabid species and as a source habitat, as several predator species activity-density responded positively to the increased area of minimum tillage fields in the surroundings. We detected a comparable positive effect of the area of flower strips in the surrounding for some species, suggesting that these linear features could also act as a temporary refuge and source. These findings highlight that sustaining diverse communities of carabid beetles and spiders probably requires adopting several and complementary management options and that in-field farming practice represent a powerful lever to foster natural enemies populations.
在耕地农业中加强多面手节肢动物捕食者有助于害虫控制和减少农药使用,并可通过田间和农场规模的多种管理选择来实现。然而,大多数研究将管理选择与循环成虫的密度联系起来,这对潜在的源汇动态和能够越冬的“源”栖息地的发生知之甚少。在这项研究中,我们评估了在不同耕作制度(最少耕作与常规耕作)下无农药冬播作物连续镶嵌的4个月内出现的和循环的地栖瓢虫和蜘蛛的组合。结果表明,在花带中,野蜘蛛和蜘蛛的越冬密度明显高于邻近花带,而邻近花带很少成为首选越冬生境。然而,我们的研究结果表明,在这两个分类群中,不同的物种表现出不同的响应和源库动态。我们的研究结果还表明,免耕田在最小耕作条件下的关键作用,既是一些优势瓢虫的优质越冬场所,也是一个来源栖息地,因为一些捕食者物种的活动密度与周围最小耕作面积的增加呈正相关。我们发现,对于一些物种来说,周围花带的面积也有类似的积极影响,这表明这些线性特征也可以作为临时避难所和来源。这些发现强调,维持瓢虫和蜘蛛的多样化群落可能需要采用几种互补的管理方案,而田间耕作实践是促进天敌种群的有力杠杆。
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引用次数: 0
Organic fertilization and hayseed application in traditional hay meadows: A pathway to biodiversity and ecological sustainability 传统草甸有机施肥与草籽施用:生物多样性与生态可持续性的途径
IF 6.4 1区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2026-01-21 DOI: 10.1016/j.agee.2026.110214
Monika Janišová , Polina Dayneko , Martin Magnes , Dariia Borovyk , Lubov Borsukevych , Denys Vynokurov , Katarína Devánová , Anna Kuzemko , Corina Iosif , Anamaria Iuga , Iveta Škodová
In Central Europe, low-intensity and low-input agriculture has fostered biodiverse landscapes and species-rich semi-natural grasslands, but implementation and ecological context of historical land-use practices remain poorly understood. We investigated meadow management practices that enhance hay productivity and quality in Poienile de sub Munte, Romania, a village with well-maintained historical land-use patterns. Specifically, we examined: (i) the implementation of manuring, ash fertilization, and hayseed application; (ii) their effects on plant species composition and diversity; and (iii) their potential in modern grassland conservation and restoration. Our study analyzed 47 vegetation plots, 30 hayseed samples, and land-use data from 30 farmers. Manure was mainly applied on gentle slopes, whereas ash was used in steep and hard-to-access areas. Hayseed was applied in 47 % of one-cut meadows and 33 % of two-cut meadows. Vegetation composition was primarily influenced by mowing frequency, soil calcium content, and the frequency of manuring and ash fertilization. Manuring decreased species richness by seven species through competitive exclusion favoring nitrogen-demanding species. In contrast, ash fertilization promoted oligotrophic specialists, including rare and vulnerable species, while increasing species richness by approximately seven species. Regular hayseed application enhanced richness through propagule supply without significant effect on vegetation composition. Hayseed composition differed from standing vegetation and was influenced by mowing time, with first-cut hay containing significantly more species and marginally more seeds than second-cut hay. Hayseed application creates a resilient system through continuous local seed reintroduction, effectively buffering historical disturbances and management variations. While this homogenizes vegetation composition across landscapes, it simultaneously maintains local diversity through enhancing functional connectivity, further supported by manuring and grazing. Traditional practices offer low-cost, accessible tools for restoring degraded grasslands, particularly when combining ash fertilization with locally-sourced hayseed under appropriate grazing regimes.
在中欧,低强度和低投入的农业培育了生物多样性景观和物种丰富的半自然草地,但对历史土地利用实践的实施和生态背景仍知之甚少。我们在罗马尼亚的Poienile de sub Munte村调查了提高干草生产力和质量的草甸管理措施,这是一个保持良好历史土地利用模式的村庄。具体来说,我们检查了:(i)实施施肥,灰施肥和干草施用;(ii)它们对植物物种组成和多样性的影响;(三)在现代草地保护与恢复中的潜力。我们的研究分析了47个植被样地、30个干草样本和30个农民的土地利用数据。肥料主要用于平缓的斜坡,而灰则用于陡峭和难以接近的地区。在47% %的一次刈割草地和33% %的两次刈割草地上施用草籽。植被组成主要受刈割频率、土壤钙含量、施肥和灰施肥频率的影响。施肥通过竞争排斥减少了7种物种的丰富度,有利于需氮物种。相比之下,灰施肥促进了贫营养专家,包括稀有和脆弱物种,同时增加了大约7种物种的丰富度。定期施用草籽可通过繁殖体供应增加丰富度,但对植被组成无显著影响。干草组成与立地不同,受刈割时间的影响,第一次刈割的干草比第二次刈割的干草含有更多的物种和略多的种子。Hayseed应用通过持续的本地种子重新引入创建了一个有弹性的系统,有效地缓冲了历史干扰和管理变化。虽然这使整个景观的植被组成同质化,但它同时通过增强功能连通性来保持当地的多样性,并进一步得到施肥和放牧的支持。传统做法为恢复退化的草原提供了低成本、容易获得的工具,特别是在适当的放牧制度下,将灰施肥与当地来源的干草结合起来。
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引用次数: 0
Effects of short-term grazing prohibition on methane, nitrous oxide and nitric oxide fluxes from alpine meadows on eastern Tibetan Plateau 短期禁牧对青藏高原东部高寒草甸甲烷、氧化亚氮和一氧化氮通量的影响
IF 6.4 1区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2026-01-21 DOI: 10.1016/j.agee.2026.110259
Xiao Chen , Rui Wang , Lei Ma , Han Zhang , Zhisheng Yao , Kai Wang , Wei Zhang , Siqi Li , Shenghui Han , Chunyan Liu , Yong Li , Xunhua Zheng
Short-term grazing prohibition (STGP) is a common practice to restore degraded alpine meadows. But its effects on emissions of greenhouse gas (GHG) and reactive nitrogen gases remain ambiguous, particularly regarding year-round dynamics and net climate impacts. Here, we address these knowledge gaps by investigating STGP′s influence on methane (CH4), nitrous oxide (N2O) and nitric oxide (NO) in an alpine meadow on the Tibetan Plateau with a field trial comparing the conventionally grazed and short-term-ungrazed treatments. Using static opaque chamber methods, we year-roundly measured dynamical fluxes of these gases in the second full year of grazing prohibition. However, the net climate impact of STGP remains uncertain due to the lack of diurnal flux measurements and concurrent CO2 exchange data. The STGP practice significantly (P < 0.001) increased CH4 uptake by 48 % annually and by 51 % in growing season. It significantly (P < 0.001) raised N2O emissions by 124 % annually and by 191 % in non-growing season while obviously (P < 0.05) reducing NO emissions by about 63 % in growing season. Notably, the STGP-stimulated N2O emissions surged by 288 % during freeze-thaw periods (P < 0.001). In addition, STGP tended to reduce temperature sensitivity for CH4 uptakes in non-growing season and for N2O and NO emissions in growing season. Notably, the CO2-equivalent balance reveals a trade-off: while the aggregate of CH4 and N2O remained a net sink at the 20-year horizon, it shifted to a source at the 100-year horizon, with STGP increasing the net positive emission by nearly 5-fold. This suggests that the climate benefit of enhanced CH4 uptake could be offset by intensified N2O emissions over the long term. However, the net climate impact of STGP still remains uncertain due to methodological constraints, including the use of static opaque chambers (which exclude diurnal and plant‑mediated fluxes) and the lack of simultaneous CO2 exchange measurements. Future studies integrating complementary methods and longer‑term monitoring are needed to fully quantify STGP′s impact on net ecosystem GHG balance.
短期禁牧是恢复退化高寒草甸的常用措施。但其对温室气体(GHG)和活性氮排放的影响仍不明确,特别是在全年动态和净气候影响方面。在此,我们通过比较常规放牧和短期不放牧的田间试验,研究了STGP对青藏高原高寒草甸甲烷(CH4)、氧化亚氮(N2O)和一氧化氮(NO)的影响,以解决这些知识空白。采用静态不透明室方法,在禁牧第二年全年对这些气体的动态通量进行了全年测量。然而,由于缺乏日通量测量和同步的CO2交换数据,STGP的净气候影响仍然不确定。STGP做法显著(P <; 0.001)增加了每年48 %的CH4吸收量,在生长季节增加了51 %。显著(P <; 0.001)提高N2O年排放量124 %,非生长期显著(P <; 0.05)降低NO年排放量约63 %。值得注意的是,在冻融期间,stgp刺激的N2O排放量激增了288 % (P <; 0.001)。此外,STGP有降低非生长季CH4吸收和生长季N2O和NO排放温度敏感性的趋势。值得注意的是,co2当量平衡揭示了一种权衡:虽然CH4和N2O的总量在20年水平上仍然是净汇,但它在100年水平上转变为源,STGP使净正排放增加了近5倍。这表明,从长期来看,CH4吸收增加的气候效益可能被N2O排放增加所抵消。然而,由于方法上的限制,包括使用静态不透明室(不包括日通量和植物介导的通量)和缺乏同步的二氧化碳交换测量,STGP的净气候影响仍然不确定。未来的研究需要整合互补方法和长期监测,以充分量化STGP对生态系统净温室气体平衡的影响。
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引用次数: 0
Natural habitats enhance pollination services in apple and sweet cherry orchards: Evidence from Southern Mediterranean agroecosystems 自然栖息地增强了苹果和甜樱桃园的授粉服务:来自南地中海农业生态系统的证据
IF 6.4 1区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2026-01-20 DOI: 10.1016/j.agee.2026.110220
Camila B. García , Keira Dymond , Manuel López-Aliste , Pablo Díaz-Siefer , Francisco E. Fontúrbel , Michael P.D. Garratt , Juan L. Celis-Diez
Wild pollinators play a critical role in crop production. However, the ongoing loss of natural habitats threatens pollination services and food security. We evaluated how the percentage of surrounding natural habitat and the distance from orchard edges influence wild floral visitors and pollination deficit in sweet cherry and apple orchards in Mediterranean Chile. Additionally, we examined the relationship between floral visitation and fruit production. We studied eighteen orchards of each crop and classified them into three categories according to the percentage of natural habitats within a 1 km radius: < 35 %, 36–70 % and > 70 %. Three pollination treatments (supplementary, open, and exclusion) were applied at the edge and into the orchard interior. We quantified floral visitor richness, floral visits, fruit set and fruit quality. Our results showed that sweet cherry orchards with less than 35 % surrounding natural habitat had significantly lower wild insect species richness and floral visits compared to orchards with > 35 %. In both crops, species richness and wild floral visits declined with increasing distance from the orchard edge. Wild floral visitors enhanced fruit set in both crops, while honey bees contributed to sweet cherry fruit set and apple seed set. Although insect pollination improved fruit quality, we found no direct relationship between flower visitation and fruit quality. In apple orchards with > 70 % natural habitat, fruit weight deficit was lower at the edge compared to both the interior and the edges of orchards with < 35 % of natural habitat. We recommend conserving natural habitats around orchards to support wild pollinator diversity and visitation, thereby reducing pollination deficits.
野生传粉媒介在作物生产中起着至关重要的作用。然而,自然栖息地的持续丧失威胁着授粉服务和粮食安全。我们评估了智利地中海地区甜樱桃和苹果园周围自然栖息地的百分比和距离果园边缘的距离如何影响野生花卉游客和授粉赤字。此外,我们还研究了开花与果实产量之间的关系。我们研究了每种作物的18个果园,并根据1 km半径内自然栖息地的百分比将其分为三类:<; 35 %,36-70 %和>; 70 %。在果园边缘和果园内部分别进行了补充授粉、开放授粉和隔离授粉三种授粉处理。我们量化了访花丰富度、访花次数、坐果量和果实质量。结果表明,与环绕自然生境35% %以下的果园相比,环绕自然生境35% %以下的甜樱桃果园野生昆虫种类丰富度和访花次数显著低于环绕自然生境35% %的果园。随着离果园边缘距离的增加,两种作物的物种丰富度和野生花卉到访量均呈下降趋势。野生花卉游客促进了这两种作物的坐果,而蜜蜂则促进了甜樱桃坐果和苹果坐果。虽然昆虫授粉改善了果实品质,但我们发现访花与果实品质之间没有直接关系。在自然生境为>; 70 %的苹果园中,果实重亏在边缘处比在自然生境为<; 35 %的苹果园的内部和边缘处都要低。我们建议保护果园周围的自然栖息地,以支持野生传粉者的多样性和来访,从而减少授粉缺陷。
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引用次数: 0
Beetle communities in agricultural landscapes: relative influences of climate, landscape, plant communities and agricultural practices 农业景观中的甲虫群落:气候、景观、植物群落和农业实践的相对影响
IF 6.4 1区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2026-01-20 DOI: 10.1016/j.agee.2026.110252
B. Penel , L. Genty , C. Marty , A. Bourdonné , A.-L. Clamens , L. Benoit , L. Soldati , A. Migeon , G.J. Kergoat , J. Haran , G. Fried , C.N. Meynard
Agricultural field margins are semi-natural habitats that play a key role in conservation and restoration, supporting threatened biodiversity of agroecosystems. However, most research on field margin biodiversity has focused on plant communities, while insect populations remain largely understudied. To address this gap, we leveraged a national monitoring network across France to provide a comprehensive taxonomic and functional characterisation of beetles, a highly diverse insect group of significant agricultural interest. We examined how climate, landscape, vegetation and agricultural practices influence the structure of field margin beetle communities. Using a combination of molecular and morphological approaches and multivariate analyses, we investigated beetle communities within the herbaceous field margins of 374 agricultural sites across continental France sampled between 2020 and 2023. Our surveys revealed a high diversity of beetles, with 797 species recorded, including hundreds of flower visitors and auxiliary species. Estimates based on accumulation curves suggest a richness of up to 1200 species, corresponding to approximately 10 % of the French beetle fauna. We also identified five community types, each having specific taxonomic and functional characteristics and associated with specific climatic, soil, landscape and agricultural environments. While large-scale climatic gradients were the main drivers shaping these community types, local vegetation played a key role in determining species richness. By contrast, agricultural practices appear to be an important structuring factor for both community types and richness. Finally, this study provides the first in-depth characterisation of beetle communities in French field margins, offering a solid baseline for future research and improving our understanding of the complex interactions among climatic, landscape, vegetation, and agricultural drivers.
农田边缘是半自然栖息地,在保护和恢复中发挥关键作用,支持受威胁的农业生态系统生物多样性。然而,对野外边缘生物多样性的研究大多集中在植物群落上,而对昆虫种群的研究还远远不够。为了解决这一差距,我们利用法国全国监测网络提供甲虫的全面分类和功能特征,甲虫是一种高度多样化的昆虫群体,具有重要的农业利益。研究了气候、景观、植被和农业实践对田间边缘甲虫群落结构的影响。采用分子和形态学方法以及多变量分析相结合的方法,研究了2020年至2023年间法国大陆374个农业样地草本田边缘的甲虫群落。我们的调查显示,甲虫的多样性很高,记录了797种,其中包括数百种花卉访客和辅助物种。根据累积曲线估计,其丰富度可达1200种,约占法国甲虫动物群的10. %。我们还确定了5种群落类型,每种类型都具有特定的分类和功能特征,并与特定的气候、土壤、景观和农业环境有关。虽然大尺度气候梯度是形成这些群落类型的主要驱动因素,但当地植被在决定物种丰富度方面发挥了关键作用。相比之下,农业实践似乎是群落类型和丰富度的重要结构因素。最后,本研究首次对法国田间边缘的甲虫群落进行了深入的描述,为未来的研究提供了坚实的基础,并提高了我们对气候、景观、植被和农业驱动因素之间复杂相互作用的理解。
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引用次数: 0
Intercropping cotton with wheat or maize enhances biological pest control but only intercropping with wheat improves cotton yield 棉花与小麦或玉米间作能有效防治害虫,但仅与小麦间作能提高棉花产量
IF 6.4 1区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2026-01-20 DOI: 10.1016/j.agee.2026.110247
Shuangshuang Jia , Enjing Hu , Minlong Li , Bing Liu , Long Yang , Wopke van der Werf , Felix J.J.A. Bianchi , Yanhui Lu
Intercropping is a promising strategy for ecologically based pest management in field crops. However, its effects on pest suppression and crop yield vary across species combinations, requiring experiments to identify context-specific driving principles and guide practical application. We evaluated the effects of intercropping cotton with spring wheat or maize on pest suppression and yield. Aphid and natural enemy populations were monitored in intercropped and sole cotton, and predation activity was assessed using cotton bollworm egg cards. Cotton yield was also measured. Aphid densities on cotton were 63 % lower in both cotton-wheat and cotton-maize than in sole cotton in 2023, and 24 % and 29 % lower in cotton-wheat and cotton-maize than in sole cotton in 2024. No significant difference was observed in cotton aphid control between the two intercrops in either year. Ladybeetle densities during early cotton aphid population build-up were higher in the two intercrops than in sole cotton. The predation rate on cotton bollworm eggs was 10 % - 178 % higher in intercrops with wheat and maize. Cotton yield was 18 % higher in cotton-wheat but 18 % lower in cotton-maize than in sole cotton. Different strength of interspecific plant competition is the likely key driver for the difference in yield impact of the two companion species. We conclude that both spring wheat and maize enhance natural enemies and reduce aphid populations in cotton, but only intercropping with wheat enhanced cotton yield. Results indicate that cotton-cereal intercropping is a robust strategy to suppress cotton aphid and reduce reliance on insecticides, but interspecific plant competition needs to be considered to ensure cotton yield.
间作是一种很有前途的大田作物有害生物生态治理策略。然而,其对害虫抑制和作物产量的影响因物种组合而异,需要通过实验来确定具体情况的驱动原理并指导实际应用。评价了春小麦间作棉花和玉米间作对害虫的抑制作用和产量的影响。采用棉铃虫卵卡法对间作棉田和单作棉田蚜虫和天敌种群进行监测,评价棉铃虫的捕食活动。同时测定了棉花产量。棉花蚜虫密度在2023年棉麦和棉玉米中均比单棉低63 %,棉麦和棉玉米中分别比单棉低24 %和29 %。两间作对棉蚜的防治效果无显著差异。棉蚜种群积累初期,两间作的瓢虫密度均高于单作。小麦、玉米间作对棉铃虫虫卵的取食率高出10 % ~ 178 %。棉麦混播的棉花产量比单棉高18 %,棉玉米混播的产量比单棉低18 %。种间竞争强度的不同可能是导致两伴生种产量影响差异的主要原因。综上所述,春小麦和玉米均能提高棉花的天敌数量,减少蚜虫数量,但只有与小麦间作才能提高棉花产量。结果表明,棉粮间作是抑制棉蚜和减少对杀虫剂依赖的有效策略,但需要考虑种间植物竞争以保证棉花产量。
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引用次数: 0
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Agriculture, Ecosystems & Environment
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