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Conventional Beef Cattle Farming Practices in Wolaita and Sidama, Southern Ethiopia 埃塞俄比亚南部Wolaita和Sidama的传统肉牛养殖方法
Q3 Veterinary Pub Date : 2023-03-01 DOI: 10.3844/ajavsp.2023.199.209
Abenezer Wendimu, Wondimagegnehu Tekalign
Beef cattle farming is an integral part of livelihood for rural and urban smallholder farmers in developing countries. We aimed to describe the cattle farming practices in selected communities of Diguna Fango district, Wolaita Zone, and Sidama Region, Southern Ethiopia. Data were collected with pretested, semi-structured questionnaires applying a cross-sectional study. A stratified sampling was used to select a total of 180 fatteners from the three communities. Collected data were analyzed using descriptive statistics. The most cited forages for fattening cattle in the dry season were hay and wheat seeds, 180 (100% each), and fresh kidney bean and maize shoot system in the wet season, 180 (100% each). Rivers (75%) and ponds (84%) were the dominant sources of water. The body frame selection criteria had a significant (p = 0.021) effect on the weight gain and determined the fattening period. The breed that was most preferred (100%) was Wolaita sangga. Trypanosomiasis (57.77%) was the highest disease burden in the study area. Lack of clean potable water (50%), feeding resources (20%), and animal health ailments (10%) were the main constraints on beef cattle farming practices in the study area. Market actors were producers, consumers, middlemen, restaurant owners, traders, and butchers. The majority (79%) of fatteners had access to market information before the sale and the price was self-determined by fatteners (78%). Availability of better meat types, cattle, and high market demand were identified as good opportunities which could be used to enhance the performance of cattle fattening activity in the study area. It should be essential to provide farmers with adequate training and extension services on improved cattle fattening technology, management techniques, and market information to increase the revenue of the farming communities that use seasonally available feed supplies.
肉牛养殖是发展中国家农村和城市小农生计的重要组成部分。我们的目的是描述埃塞俄比亚南部迪古纳法戈区、沃莱塔区和西达马区选定社区的养牛做法。数据收集采用预测试,半结构化问卷采用横断面研究。采用分层抽样的方法,从三个群落中选取180名育肥者。收集的数据采用描述性统计进行分析。旱季肥育牛引用最多的饲料是干草和小麦种子,各180份(100%),湿季鲜芸豆和玉米芽系统,各180份(100%)。河流(75%)和池塘(84%)是主要的水源。体框选择标准对增重有显著影响(p = 0.021),并决定了育肥期。最受欢迎的品种是Wolaita sangga(100%)。研究区疾病负担最高的是锥虫病(57.77%)。缺乏清洁饮用水(50%)、饲料资源(20%)和动物健康疾病(10%)是研究地区肉牛养殖的主要制约因素。市场参与者包括生产者、消费者、中间商、餐馆老板、商人和屠夫。大多数(79%)育肥者在销售前获得了市场信息,价格由育肥者自行决定(78%)。在研究区域,更好的肉类品种、牛的可用性和高市场需求被认为是提高牛育肥活动性能的良好机会。必须向农民提供关于改良的牛育肥技术、管理技术和市场信息的充分培训和推广服务,以增加使用季节性饲料供应的农业社区的收入。
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引用次数: 1
Endometritis Control and Enhanced Reproductive Performance in Aceh Cow Using Medicinal Plants 利用药用植物控制亚齐奶牛子宫内膜炎和提高生殖性能
Q3 Veterinary Pub Date : 2023-03-01 DOI: 10.3844/ajavsp.2023.235.242
Teuku Armansyah, Teuku Armansyah, Tongku Nizwan Siregar, Tongku Nizwan Siregar, Amalia Sutriana, Suhartono Suhartono, Budianto Panjaitan, Rania Evita Agustine, Nuriani Saputri, Uti Nurlita
Antibiotic treatment for endometritis has some weaknesses due to its toxicity and unexpected side effects. Therefore, the utilization of plant extract as antimicrobials is an alternative to antibiotics. We aimed to evaluate the efficacy of betel leaf, moringa leaf, and garlic extracts in treating endometritis in Aceh cows. We used nine Aceh cows at 3-5 years old, 150-250 kg body weight, and were diagnosed with endometritis through the White Side Test (WST). All cows were injected with 25 mg Prostaglandin (PGF2α) hormone and cervical mucus was collected 8-12 h post-estrus onset. A 20 mL of red betel leaf, moringa leaf, and garlic extracts with a concentration of 40% was administered every 24 h for 7 days. The extract application was carried out using an insemination gun inserted into the uterus. Post-treatment, the cows were re-synchronized with PGF2α and artificially inseminated after showing symptoms of estrus. Pregnancy was diagnosed using rectal palpation 60-90 days post-insemination. WST score data and total bacterial counts were analyzed using the Wilcoxon test, whereas the reproductive performance data were reported descriptively. The results showed that the administration of betel leaf, moringa leaf, and garlic extracts tended to reduce the WST scores and bacterial counts. The successful pregnancy rates in groups treated with betel leaf extract, moringa leaf, and garlic extract were 66.6, 66.6, and 0.0%, respectively. The application of red betel leaf extract and moringa leaf could reduce the level of endometritis and increase the pregnancy rates in Aceh cows.
抗生素治疗子宫内膜炎由于其毒性和意想不到的副作用有一些弱点。因此,利用植物提取物作为抗菌剂是抗生素的替代品。我们旨在评估槟榔叶、辣木叶和大蒜提取物治疗亚齐奶牛子宫内膜炎的疗效。我们选用了9头3-5岁、体重150-250公斤的亚齐奶牛,并通过白侧试验(WST)诊断为子宫内膜炎。所有奶牛均注射前列腺素(PGF2α)激素25 mg,并于发情后8-12 h采集宫颈粘液。每24 h给予红槟榔叶、辣木叶和大蒜提取物20 mL,浓度为40%,连续7天。使用插入子宫的人工授精枪进行提取物应用。处理后,奶牛再次与PGF2α同步,并在出现发情症状后进行人工授精。在人工授精后60-90天用直肠触诊诊断妊娠。WST评分数据和细菌总数采用Wilcoxon检验进行分析,而繁殖性能数据采用描述性报告。结果表明,槟榔叶、辣木叶和大蒜提取物有降低WST评分和细菌计数的趋势。槟榔叶提取物组、辣木叶提取物组和大蒜提取物组的妊娠成功率分别为66.6、66.6和0.0%。应用红槟榔叶提取物和辣木叶提取物可降低亚齐奶牛子宫内膜炎水平,提高妊娠率。
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引用次数: 0
Prevalence and Risk Factors of Bovine Fascioliasis in Northeastern Peru 秘鲁东北部牛片形吸虫病的流行及危险因素
Q3 Veterinary Pub Date : 2023-03-01 DOI: 10.3844/ajavsp.2023.173.183
Clavel Diaz-Quevedo, Clavel Diaz-Quevedo, Hugo Frias, Nilton Luis Murga Valderrama, Lenin Torres Bernal, Ilse Silvia Cayo Colca, José Américo Saucedo-Uriarte, José Américo Saucedo-Uriarte
Bovine fascioliasis in Peru is highly prevalent in almost all regions; however, there are few studies about its prevalence in the region of Amazonas. This research aimed to determine the prevalence and risk factors associated with fascioliasis from four livestock basins in the Amazonas region. A total of 941 bovine feces samples were analyzed and a prevalence of 52% was found. The highest prevalence was registered in females (53.9%), crossbred (58.6%), and producers with less than 50 animals (54.40%). The highest risk factor was for Brown Swiss (2.1), crossbreeds (2.4), heifer (4.1), females (1.4), and bovine that drinks water from streams (2.5) and waterhole (2.4). With the principal component analysis, 5 groups were identified, where the first explains that the area of the farm and the number of animals are related to the prevalence of fascioliasis. Group five indicated a relationship between the drinking water source and the breeds with the highest prevalence of fascioliasis. The results show that there are high prevalence and risk factors that affect livestock productivity and welfare. For this reason, there is a need to improve veterinary and animal health support, as well as training in livestock management, providing adequate sources of nutrition, and improving drug administration.
秘鲁几乎所有地区都高度流行牛片吸虫病;然而,关于其在亚马逊地区的流行情况的研究很少。本研究旨在确定亚马逊地区4个畜牧业盆地的片形吸虫病患病率及其相关危险因素。共分析941份牛粪便样本,发现感染率为52%。发病率最高的是雌性(53.9%)、杂交(58.6%)和少于50头的养殖户(54.40%)。危险系数最高的是瑞士棕色牛(2.1)、杂交牛(2.4)、小母牛(4.1)、母牛(1.4)和饮用溪流水的牛(2.5)和水坑水的牛(2.4)。通过主成分分析,确定了5个组,其中第一个组解释了农场面积和动物数量与片形吸虫病的流行有关。第五组表明饮用水源与片吸虫病患病率最高的品种之间存在相关性。结果表明,该地区存在高患病率和影响畜禽生产力和福利的危险因素。因此,有必要改善兽医和动物健康支助,以及牲畜管理方面的培训,提供充足的营养来源,并改进药物管理。
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引用次数: 0
Breeding Qualities of Aberdeen-Angus Cows of Different Genotypes in Northern Kazakhstan 哈萨克斯坦北部不同基因型阿伯丁-安格斯奶牛的育种品质
Q3 Veterinary Pub Date : 2023-03-01 DOI: 10.3844/ajavsp.2023.217.222
Pavel Shevchenko, Raushan Rychshanova, Kulyay Suleimanova, Zhanaidar Bermukhametov, Jan Miciński, Inna Brel-Kisselevа
The Aberdeen-Angus breed of cattle is widely used to increase the number of breeding cores and beef production volumes and improve its quality in the Republic of Kazakhstan. The purpose of the research is to study the breeding and adaptive qualities of Aberdeen-Angus cattle in the conditions of the Kostanay region (Northern region of the Republic of Kazakhstan). To achieve this goal, we studied the breeding qualities of imported breeding stock Aberdeen-Angus cows and their breeding use as factors in the formation of productivity in future offspring. In the conditions of Northern Kazakhstan, the breeding qualities of this breed were studied depending on the country of origin. The experimental part of the work was carried out in 2019-2022. Two groups were formed: Group I (n = 288 heads) Kazakh breeding and Group II (n = 100 heads) Estonian breeding, which was grown under the same conditions of feeding and maintenance in "Kolos-firma” LLP of Kostanay region. According to all the considered breeding qualities, superiority was observed in cattle of Estonian breeding.
在哈萨克斯坦共和国,阿伯丁-安格斯品种的牛被广泛用于增加育种核心的数量和牛肉产量,并提高其质量。本研究的目的是研究阿伯丁-安格斯牛在Kostanay地区(哈萨克斯坦共和国北部地区)条件下的繁殖和适应品质。为了实现这一目标,我们研究了进口阿伯丁-安格斯奶牛的育种品质及其育种利用作为未来后代生产力形成的因素。在哈萨克斯坦北部的条件下,根据原产国研究了该品种的育种品质。这项工作的实验部分于2019-2022年进行。分为两组:第一组(n = 288头)哈萨克种猪,第二组(n = 100头)爱沙尼亚种猪,在Kostanay地区“Kolos-firma”LLP相同的饲养和养护条件下生长。根据所有考虑的育种品质,在爱沙尼亚育种的牛中观察到优势。
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引用次数: 0
Resistance to Antibacterial Agents in Escherichia coli Isolated from Domestic Cats and Dogs in the Northern Region of the Republic of Kazakhstan 哈萨克斯坦共和国北部地区家猫和家狗分离的大肠杆菌对抗菌药物的耐药性
Q3 Veterinary Pub Date : 2023-03-01 DOI: 10.3844/ajavsp.2023.190.198
Anara Yeleussizova, Yuliya Aleshina, Anara Mendybayeva, Madina Khassanova, Raushan Rychshanova
The widespread use of antimicrobial agents for pets in veterinary practice has led to the emergence and spread of drug resistance not only in pathogenic but also in opportunistic bacteria. The study aims to evaluate the profile of resistance to antibacterial agents in Escherichia coli strains isolated from domestic cats and dogs, as well as to look into the determinants of resistance responsible for the ability of microorganisms to resist the effects of antimicrobial agents. During 2021-2022, biological material from cats and dogs, taken in veterinary clinics of Kostanay, Republic of Kazakhstan, was analyzed. Identification of genes encoding antimicrobial resistance was carried out by Polymerase Chain Reaction (PCR). As a result, it was found that all isolated strains of microorganisms showed sensitivity to the action of meropenem included in the group of beta-lactam antibiotics and showed resistance to tetracycline, doxycycline, ofloxacin, ampicillin, and amoxicillin. Resistance genes were identified for all the studied groups of antibiotics in the DNA of E. coli isolated from cats and no genes encoding resistance to fluoroquinolones were detected in the DNA of strains isolated from dogs. The most common genes were genes encoding resistance to aminoglycosides (43.8%), tetracyclines (35%), beta-lactams (33.5%), sulfonamides (16.1%), and fluoroquinolones (1.24%). In general, domestic cats and dogs have a high prevalence of E. coli strains resistant to beta-lactam drugs (ampicillin: 40%, amoxicillin: 36%) and tetracyclines (tetracycline: 71%, doxycycline: 47%). Resistance in most cases was explained by the presence of such resistance genes as blaTEM, OXA, tetA, and tetB.
在兽医实践中,宠物抗菌药物的广泛使用导致耐药性的出现和传播,不仅在致病性细菌中,而且在机会性细菌中。该研究旨在评估从家猫和狗身上分离的大肠杆菌菌株对抗菌剂的耐药性概况,并探讨微生物抵抗抗菌剂作用能力的耐药性决定因素。在2021-2022年期间,对在哈萨克斯坦共和国科斯坦内兽医诊所采集的猫和狗的生物材料进行了分析。采用聚合酶链式反应(PCR)鉴定耐药基因。结果发现,所有分离的微生物菌株对β -内酰胺类抗生素组中的美罗培南均表现出敏感性,对四环素、多西环素、氧氟沙星、氨苄西林和阿莫西林均表现出耐药性。从猫分离的大肠杆菌DNA中鉴定出所有研究类群的抗生素耐药基因,而从狗分离的大肠杆菌DNA中未检测到氟喹诺酮类药物耐药基因。最常见的基因是氨基糖苷类耐药基因(43.8%)、四环素类耐药基因(35%)、内酰胺类耐药基因(33.5%)、磺胺类耐药基因(16.1%)和氟喹诺酮类耐药基因(1.24%)。一般来说,家猫和狗对-内酰胺类药物(氨苄西林:40%,阿莫西林:36%)和四环素类药物(四环素:71%,多西环素:47%)耐药的大肠杆菌菌株的患病率很高。在大多数情况下,耐药是由这些耐药基因的存在,如blaTEM, OXA, tetA和tetB。
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引用次数: 0
Influence of Various Schemes of Feeding Calves with Whole Milk on their Growth Indicators and Physiological State 不同全脂奶喂养方案对犊牛生长指标和生理状态的影响
Q3 Veterinary Pub Date : 2023-03-01 DOI: 10.3844/ajavsp.2023.210.216
K. K. Akhazhanov, T. K. Bekseitov, J. J. Uakhitov, B. Ateikhan, M. V. Syrovatskiy, S. V. Beketov
In dairy farming, special requirements are imposed on the feeding of replacement young animals. The period when young animals receive colostrum and milk is considered important for raising viable and productive animals. During this time, a large amount of whole milk is consumed by calves. In this connection, the purpose of our study was to compare the effect of various schemes of feeding calves with whole milk on their growth indicators and physiological state. The study was conducted in the Pobeda Limited Liability Partnership (Pavlodar region, Republic of Kazakhstan). The objects of the study were heifers of the Simmental breed. During the experiment, the authors evaluated the effect of the commercial (375 L) and reduced (345 and 285 L) whole milk feeding schemes on the subsequent growth and development of calves. In just 150 days of the experimental period, the highest average weight, as well as the largest absolute and average daily gains were noted in animals that received the least amount of milk according to the feeding scheme, which became possible due to the early and more intensive consumption of compound feed by calves of these groups. In total, in the group that received 375 L of milk, each calf received 160.97 kg of starter compound feed, in the group with 345 L of milk 176.45 kg of compound feed, and the group with 285 L of milk 215 kg of compound feed. Thus, with the same nutritional value of the diets, a decrease in the amount of whole milk fed to the calves of the experimental groups led to an increase in their consumption of compound feed, which, against the background of a normal physiological state and the absence of significant differences in the growth dynamics of experimental animals, makes it economically feasible to approach the use of such expensive feed as whole milk.
在奶牛养殖中,对替代幼畜的喂养有特殊要求。幼年动物接受初乳和乳汁的时期被认为对饲养有活力和多产的动物很重要。在此期间,小牛消耗了大量的全脂牛奶。因此,本研究的目的是比较不同全脂奶喂养方案对犊牛生长指标和生理状态的影响。这项研究是在Pobeda有限责任合伙企业(哈萨克斯坦共和国巴甫洛达尔地区)进行的。研究对象是西门塔尔品种的小母牛。在试验中,作者评估了商业(375升)和减少(345升和285升)全脂牛奶饲喂方案对犊牛后续生长发育的影响。在仅150天的试验期内,根据饲喂方案,哺乳量最少的动物平均体重最高,绝对日增重和平均日增重最大,这可能是由于这些组的犊牛较早且更密集地消耗了配合饲料。综上所述,饲喂375 L乳汁组每头犊牛饲喂起始配合饲料160.97 kg,饲喂345 L乳汁组每头犊牛饲喂配合饲料176.45 kg,饲喂285 L乳汁组每头犊牛饲喂配合饲料215 kg。由此可见,在饲粮营养价值相同的情况下,减少试验组犊牛全脂奶的摄取量会导致犊牛配合饲料摄取量的增加,在正常生理状态和实验动物生长动态无显著差异的背景下,采用全脂奶这种昂贵饲料的做法在经济上是可行的。
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引用次数: 0
Morphometric Differentiation of Indigenous Tswana Goat Populations in Botswana Using Canonical Discriminant Analysis 博茨瓦纳土著茨瓦纳山羊种群形态的典型判别分析
Q3 Veterinary Pub Date : 2023-03-01 DOI: 10.3844/ajavsp.2023.184.189
Ditiro Maletsanake
Indigenous goats are renowned for their endurance and capacity to withstand extreme climatic conditions. To categorize indigenous animal genetic resources, morphometric measurements have been employed to analyze the properties of numerous farm animal populations and breeds. The purpose of this research was to use canonical discriminant analysis to categorize the diverse Tswana goat populations in Botswana based on their morphometric characteristics. A total of 1324 does were sampled from the five agroecological regions of Botswana. Only goats with Four Pairs of Permanent Incisors (4PPI = 48 months) were considered to minimize age effects. A hanging scale was used to measure body weight and other body parameters were measured using a fabric measuring tape. Data on morphometric traits were analyzed using the general linear model techniques of the statistical analysis system. The CANDISC algorithm was used to calculate Mahalanobis distances between class means, canonical variables, and eigenvalues. The largest Mahalanobis distance (8.79) was observed between the indigenous goat populations of Central and Ngamiland. The Southern and Gaborone goat populations had the smallest Mahalanobis distance (1.75). The analysis among the five agro-ecological regions identified CAN1 (93.4%) to be statistically significant (p<0.001). However, CAN1 and CAN2 together constituted 97.5% of all changes among the canonical variables, showing a thorough representation of most of the indigenous goats under the study. The morphological distance results confirm that the Ngamiland goat population was morphologically distinct from the other indigenous Tswana goat populations.
本地山羊以其耐力和承受极端气候条件的能力而闻名。为了对地方动物遗传资源进行分类,形态学测量被用于分析许多农场动物种群和品种的特性。本研究的目的是利用典型判别分析,根据其形态特征对博茨瓦纳不同的茨瓦纳山羊种群进行分类。从博茨瓦纳的五个农业生态区共取样了1324只绵羊。只有山羊有4对恒门牙(4PPI = 48个月)被认为是最小的年龄影响。用吊秤测量体重,用织物卷尺测量其他身体参数。利用统计分析系统的一般线性模型技术对形态计量性状数据进行分析。使用CANDISC算法计算类均值、典型变量和特征值之间的马氏距离。中部和恩加兰本地山羊种群之间的马氏距离最大,为8.79。南部和哈博罗内山羊种群的马氏距离最小(1.75)。5个农业生态区间的分析发现,CAN1(93.4%)具有统计学显著性(p<0.001)。然而,CAN1和CAN2共同构成了所有典型变量变化的97.5%,显示了研究中大多数本地山羊的全面代表。形态距离结果证实,恩加兰山羊种群在形态上与其他土着茨瓦纳山羊种群不同。
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引用次数: 0
Marking of Genetic Resistance to Chlamydia, Brucellosis and Mastitis in Holstein Cows by Using Polymorphic Variants of LTF, MBL1 and TLR9 Genes 应用LTF、MBL1和TLR9基因多态性变异标记荷斯坦奶牛对衣原体、布鲁氏菌病和乳腺炎的遗传抗性
Q3 Veterinary Pub Date : 2023-02-01 DOI: 10.3844/ajavsp.2023.89.97
I. Beishova, Birzhan Nurgaliyev, A. Belaya, G. Chuzhebayeva, V. Ulyanov, T. Ulyanova, Alexandr Kovalchuk, L. Dushayeva, K. Murzabayev, A. Taipova, Aizhan Zholdasbekova, A. Isabaev
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引用次数: 0
Safe Technologies of Prophylactic Disinfection in the Presence of Animals 动物在场时预防性消毒的安全技术
Q3 Veterinary Pub Date : 2023-02-01 DOI: 10.3844/ajavsp.2023.107.116
Bakhyt Barakhov, Zhaxylyk Myrzabekov, Nurgul Serikbai, Dinara Narbayeva, Gulmira Alpysbayeva, Assylbek Batyrbekov
Prophylactic disinfection in the presence of animals reduces the concentration of microorganisms in the air and thereby prevents various infectious diseases. The study aims to develop a technological mode of prophylactic disinfection in the presence of animals. As part of the disinfection measures, aerosol disinfection, wet disinfection, and foam disinfection were performed. Sanitation procedures used the preparations of Dezinfekt, Penodez, Yodizol, Glutex, and bleach. The study demonstrated that after wet disinfection in the research object with the use of Penodez by the added foam method (combined method), the obtained quantitative indicators when compared to those of the control objects, indicate a 9.7% increase in disinfection effectiveness. With the use of Yodizol in aerosol disinfection in the presence of piglets, the survival rate of microorganisms in the air dropped by 96.3% and on the floor and surfaces by 95.2 and 93.0%, respectively. In the monitoring of the toxic effects of the preparation on the body, no allergic reactions are observed and the level of hemoglobin in the blood was 8.1% higher than in the control group. The experiment demonstrated the highest effectiveness of the combined disinfection method (wet + foam). The use of safe prophylactic disinfection (aerosol) in the presence of animals was found to be expedient. An eco-friendly composition disinfectant based on iodine compounds (Yodizol) was developed for disinfection in the presence of animals. The timing of prophylactic disinfection (considering the accumulation of microorganisms on various surfaces and in the air in facilities) is established.
在动物在场的情况下进行预防性消毒,可以降低空气中微生物的浓度,从而预防各种传染病。本研究旨在开发一种有动物在场的预防性消毒技术模式。作为消毒措施的一部分,进行了气溶胶消毒、湿法消毒和泡沫消毒。卫生程序使用Dezinfekt, Penodez, Yodizol, Glutex和漂白剂的制剂。研究表明,使用Penodez用添加泡沫法(联合法)对研究对象进行湿法消毒后,所获得的定量指标与对照对象相比,消毒效果提高了9.7%。在仔猪在场的情况下,使用Yodizol进行气溶胶消毒,空气中微生物存活率下降96.3%,地板和表面微生物存活率分别下降95.2%和93.0%。在监测制剂对机体的毒性作用时,未观察到过敏反应,血液中血红蛋白水平比对照组高8.1%。实验结果表明,湿法+泡沫法联合消毒效果最好。在动物在场的情况下使用安全的预防性消毒(气溶胶)被认为是权宜之计。开发了一种基于碘化合物(Yodizol)的环保组合消毒剂,用于动物在场消毒。确定了预防性消毒的时间(考虑到微生物在各种表面和设施空气中的积累)。
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引用次数: 0
Dietary Inclusion of Sunflower Seed Alters Blood Plasma and Endometrium Lipid Profile in Beef Cows 日粮中添加葵花籽可改变肉牛血浆和子宫内膜脂质
Q3 Veterinary Pub Date : 2023-02-01 DOI: 10.3844/ajavsp.2023.156.165
Mariângela Bueno Cordeiro Maldonado, Ricardo de Oliveira Rodrigues, Dante Pazzanese Duarte Lanna, Guilherme Pugliesi, Milton Maturana Filho, Adriano Felipe Mendes, Lucas de Oliveira Bezerra, Ricardo da Fonseca, Claudia Maria Bertan Membrive
Polyunsaturated Fatty Acids (PUFA)-enriched diets are recognized as a significant approach for improving the reproductive efficiency of cattle. The aim of this study was to evaluate the effects of sunflower seed, a PUFA-rich feed source, supplementation on plasma and endometrium lipid profiles in beef cattle. It was hypothesized that feeding of sunflower seed has an additional effect on PUFA that causes changes in the profiles of total cholesterol, Low-Density Lipoprotein (LDL), High-Density Lipoprotein (HDL), and triglycerides plasma concentrations, and endometrium fatty acids composition. For such, 60 Nelore cows had their ovulation synchronized and were then fed 1.7 kg/day/animal of control diet composed of soybean meal and corn or enriched with sunflower seed. Both diets were provided in troughs maintaining the measure of 22 linear cm/trough/animal. The cows were supplemented for 6 (D0-D5), 14 (D0-D13), or 22 days (D0-D21), according to the experimental group, from the expected estrus (D0) and blood was collected throughout the treatment. 24 h after receiving the last supplementation, 15 control, and 15 treated cows were slaughtered for analysis of blood plasma and endometrium lipid profile. The plasma lipid concentrations were assessed through the utilization of commercially available colorimetric kits employings an enzymatic method in an automated analyzer. The endometrial fatty acids profile was analyzed by gas chromatography. Treated cows presented increased total plasma cholesterol concentrations on D18 and D21; Increased HDL concentrations on D10, D14, D18, and D21; and increased LDL concentrations on D21 (p<0.05); but no difference in triglycerides. Furthermore, feeding sunflower seed to beef cows increased endometrial concentrations of C18:1 T10-T11-T12 and C10:1 and decreased those of iso-C15:0, C20:5, C20:3 n3, C23:0, C24:0 and C22:5 fatty acids. We conclude that feeding beef cows with 1.7 kg/day/animal of sunflower seed alters the lipid profile of plasma and endometrium and that such changes are potentially associated with higher reproductive efficiency.
富含多不饱和脂肪酸(PUFA)的饲粮被认为是提高牛繁殖效率的重要途径。本研究旨在评价葵花籽对肉牛血浆和子宫内膜脂质的影响。葵花籽是一种富含pufa的饲料来源。据推测,葵花籽喂养对PUFA有额外的影响,导致总胆固醇、低密度脂蛋白(LDL)、高密度脂蛋白(HDL)、甘油三酯血浆浓度和子宫内膜脂肪酸组成的变化。在此基础上,60头Nelore奶牛同步排卵,饲喂由豆粕和玉米组成的对照饲粮或添加葵花籽的对照饲粮1.7 kg/d /头。两种饲粮均在槽中饲喂,保持22线性cm/槽/头。根据试验组奶牛的预期发情(D0),分别添加6 (D0- d5)、14 (D0- d13)、22 (D0- d21) d,并在整个处理过程中采集血液。饲喂末次饲粮24 h后,屠宰15头对照组奶牛和15头处理奶牛,测定血浆和子宫内膜脂质。血浆脂质浓度通过利用市售比色试剂盒,在自动分析仪中采用酶法进行评估。气相色谱法分析子宫内膜脂肪酸谱。D18和D21处理奶牛血浆总胆固醇浓度升高;D10、D14、D18、D21的HDL浓度升高;D21的LDL浓度升高(p<0.05);但甘油三酯没有差别。另外,饲喂葵花籽可提高肉牛子宫内膜C18:1 T10-T11-T12和C10:1脂肪酸浓度,降低iso-C15:0、C20:5、C20:3 n3、C23:0、C24:0和C22:5脂肪酸浓度。由此可见,饲喂1.7 kg/d葵花籽可以改变肉牛的血浆和子宫内膜的脂质分布,这种变化可能与更高的生殖效率有关。
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American Journal of Animal and Veterinary Sciences
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