Pub Date : 2019-08-08DOI: 10.46624/ajptr.2020.v10.i3.002
Dr.P. Shalini, N. Reddy
Management of worn dentition using fixed or removable prostheses is complex and among the most difficult cases to restore. Evaluation of vertical dimension is important for the management, and comprehensive treatment plan is required for all cases. Articulated casts and diagnostic wax-up provide a important information which and helpful for the evaluation of treatment options. Minimal changes to vertical dimension of occlusion is usually confirmed with the clinical evaluation of the patient using diagnostic splint or provisional prosthesis. Nevertheless, occlusal wear of prosthetic teeth is a normal process during the lifetime of a denture. In this line, vertical dimension(VD) assessment should be conservative and with careful approach and it should not be changed. Particularly, increasing the VD for bruxism patients puts a severe overload on the teeth and it often results in the destruction of the restorations.
{"title":"Methods to Determine Vertical Jaw Relation- Review","authors":"Dr.P. Shalini, N. Reddy","doi":"10.46624/ajptr.2020.v10.i3.002","DOIUrl":"https://doi.org/10.46624/ajptr.2020.v10.i3.002","url":null,"abstract":"Management of worn dentition using fixed or removable prostheses is complex and among the most difficult cases to restore. Evaluation of vertical dimension is important for the management, and comprehensive treatment plan is required for all cases. Articulated casts and diagnostic wax-up provide a important information which and helpful for the evaluation of treatment options. Minimal changes to vertical dimension of occlusion is usually confirmed with the clinical evaluation of the patient using diagnostic splint or provisional prosthesis. Nevertheless, occlusal wear of prosthetic teeth is a normal process during the lifetime of a denture. In this line, vertical dimension(VD) assessment should be conservative and with careful approach and it should not be changed. Particularly, increasing the VD for bruxism patients puts a severe overload on the teeth and it often results in the destruction of the restorations.","PeriodicalId":7701,"journal":{"name":"American Journal of PharmTech Research","volume":"31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91300280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-08-08DOI: 10.46624/ajptr.2020.v10.i3.007
Binu Km, El-Kosasy Am, H. Doddayya, S. Antin
{"title":"Pulsatile drug delivery, circadian rhythms, Tiotropium Bromide, Lag time, Press coated tablets.","authors":"Binu Km, El-Kosasy Am, H. Doddayya, S. Antin","doi":"10.46624/ajptr.2020.v10.i3.007","DOIUrl":"https://doi.org/10.46624/ajptr.2020.v10.i3.007","url":null,"abstract":"","PeriodicalId":7701,"journal":{"name":"American Journal of PharmTech Research","volume":"44 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87419303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-08-08DOI: 10.46624/ajptr.2020.v10.i3.005
S. Mishra, R. Shankar
Bundelkhand is located Central part of India with an unique type of climatic condition having stony sandy and rocky soil deficient in water. Gradual climatic change due to acute pressure on land for various developmental activities are regularly increasing the degradation of biodiversity which is affecting the potential of medicinal plants and invite needs for the conservation of biodiversity of medicinal plants in the region. During the course of various studies authors have identified the medicinal plants of high value, used in top medical prescriptions and traditional medical practices viz. Acacia catechu (Khadira), Asparagus racemosus (Shatawari), Boswellia serrata (Shalaki), Desmodium gangeticum(Shalaparni), Gymnema sylvestre.(Retz.) R. Br. ex Sm.( Gudmaar), Hemidesmus indicus R.Br.(Anantmoola),Oroxylum indicum (L.) Kurz, Pluchia lanceolata(Rasana),Tribulus terrestris (Gokshuru), Uraria picta (Prishniparni), Withania somnifera (Ashwagandha), Writia tinctoriaetc. Efforts are needed in acclimatizing the medicinal plants in the garden land, nurseries, development of strategy of protection of natural habitat of the medicinal plants of region, developing ago-technical protocols for cultivation of medicinal plants suitable for farmers to enrich their economy as well as biodiversity under Government control is need of the day ensuring effective conservation of these valuable medicinal resources of the country. Cultivation of Cymbopogon flexuosus (Pootika),Saccharum munja (Munja), and Chrysopogon zizanioides (Ushir) is also the need of time for enriching the biodiversity through conserving the medicinal plants.
{"title":"Diversity and Conservation Strategies of Pharmaceutically important Medicinal plants of Bundelkhand region, India","authors":"S. Mishra, R. Shankar","doi":"10.46624/ajptr.2020.v10.i3.005","DOIUrl":"https://doi.org/10.46624/ajptr.2020.v10.i3.005","url":null,"abstract":"Bundelkhand is located Central part of India with an unique type of climatic condition having stony sandy and rocky soil deficient in water. Gradual climatic change due to acute pressure on land for various developmental activities are regularly increasing the degradation of biodiversity which is affecting the potential of medicinal plants and invite needs for the conservation of biodiversity of medicinal plants in the region. During the course of various studies authors have identified the medicinal plants of high value, used in top medical prescriptions and traditional medical practices viz. Acacia catechu (Khadira), Asparagus racemosus (Shatawari), Boswellia serrata (Shalaki), Desmodium gangeticum(Shalaparni), Gymnema sylvestre.(Retz.) R. Br. ex Sm.( Gudmaar), Hemidesmus indicus R.Br.(Anantmoola),Oroxylum indicum (L.) Kurz, Pluchia lanceolata(Rasana),Tribulus terrestris (Gokshuru), Uraria picta (Prishniparni), Withania somnifera (Ashwagandha), Writia tinctoriaetc. Efforts are needed in acclimatizing the medicinal plants in the garden land, nurseries, development of strategy of protection of natural habitat of the medicinal plants of region, developing ago-technical protocols for cultivation of medicinal plants suitable for farmers to enrich their economy as well as biodiversity under Government control is need of the day ensuring effective conservation of these valuable medicinal resources of the country. Cultivation of Cymbopogon flexuosus (Pootika),Saccharum munja (Munja), and Chrysopogon zizanioides (Ushir) is also the need of time for enriching the biodiversity through conserving the medicinal plants.","PeriodicalId":7701,"journal":{"name":"American Journal of PharmTech Research","volume":"76 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90836541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-08DOI: 10.46624/ajptr.2019.v9.i3.003
Priya Singh
GM derived seed is one type of powered test item. It is used in 90 day repeat oral toxicity studies. Powered is administered orally to wistar rats for a period of 90 days. This study also compared the profile of the GM derived cotton seed with the non GM derived cotton seed at the highest does tested. The study was performed to generate information on major systemic toxic effects (if any), target organs and an estimate of the No Observed Effect Level (NOEL) / No Observed Adverse Effect Level (NOAEL) and also reversibility of observed changes, if any, after discontinuation of treatment with the Test Items.
{"title":"90 Day Repeat DOSE Oral Toxicity Study of GM Derived Cotton Seed In Wistar Rats","authors":"Priya Singh","doi":"10.46624/ajptr.2019.v9.i3.003","DOIUrl":"https://doi.org/10.46624/ajptr.2019.v9.i3.003","url":null,"abstract":"GM derived seed is one type of powered test item. It is used in 90 day repeat oral toxicity studies. Powered is administered orally to wistar rats for a period of 90 days. This study also compared the profile of the GM derived cotton seed with the non GM derived cotton seed at the highest does tested. The study was performed to generate information on major systemic toxic effects (if any), target organs and an estimate of the No Observed Effect Level (NOEL) / No Observed Adverse Effect Level (NOAEL) and also reversibility of observed changes, if any, after discontinuation of treatment with the Test Items.","PeriodicalId":7701,"journal":{"name":"American Journal of PharmTech Research","volume":"70 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75803524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-08DOI: 10.46624/ajptr.2019.v9.i3.024
Ganesh M. Sanap, Prajakta S. Shirode, Kalpesh V. Sonar
To develop and validate simple, rapid, linear, accurate, precise and economical UV Spectroscopic method for estimation of Guaifenesin in tablet dosage form. The drug is freely soluble in analytical grade Methanol. The drug was identified in terms of solubility studies and on the basis of melting point done on melting point apparatus of Equiptronics. It showed absorption maxima were determined in analytical grade Methanol. The drug obeyed the Beer’s law and showed good correlation of concentration with absorption which reflect in linearity. The UV spectroscopic method was developed for estimation of Guaifenesin in tablet dosage form and also validated as per ICH guidelines. The drug is freely soluble in analytical grade Methanol, moderately soluble in Benzene and soluble in Chloroform, Glycerol. So, the analytical grade Methanol is used as a diluent in method. The melting point of Guaifenesin was found to be 78-79 ̊C (uncorrected). It showed absorption maxima 269 nm in analytical grade Methanol. On the basis of absorption spectrum the working concentration was set on 10μg/ml (PPM). The linearity was observed between 6-14 μg/ml (PPM). The results of analysis were validated by recovery studies. The recovery was found to be 98.75, 101 and 99.17% for three levels respectively. The % RSD for precision was found to be 0.97%. A simple, rapid, linear, accurate, precise and economical UV Spectroscopic method has been developed for estimation of Guaifenesin in tablet dosage form. The method could be considered for the determination of Guaifenesin in quality control laboratories.
{"title":"Development and Validation of UV Spectroscopic Method for Estimation of Guaifenesin In Tablet Dosage Form","authors":"Ganesh M. Sanap, Prajakta S. Shirode, Kalpesh V. Sonar","doi":"10.46624/ajptr.2019.v9.i3.024","DOIUrl":"https://doi.org/10.46624/ajptr.2019.v9.i3.024","url":null,"abstract":"To develop and validate simple, rapid, linear, accurate, precise and economical UV Spectroscopic method for estimation of Guaifenesin in tablet dosage form. The drug is freely soluble in analytical grade Methanol. The drug was identified in terms of solubility studies and on the basis of melting point done on melting point apparatus of Equiptronics. It showed absorption maxima were determined in analytical grade Methanol. The drug obeyed the Beer’s law and showed good correlation of concentration with absorption which reflect in linearity. The UV spectroscopic method was developed for estimation of Guaifenesin in tablet dosage form and also validated as per ICH guidelines. The drug is freely soluble in analytical grade Methanol, moderately soluble in Benzene and soluble in Chloroform, Glycerol. So, the analytical grade Methanol is used as a diluent in method. The melting point of Guaifenesin was found to be 78-79 ̊C (uncorrected). It showed absorption maxima 269 nm in analytical grade Methanol. On the basis of absorption spectrum the working concentration was set on 10μg/ml (PPM). The linearity was observed between 6-14 μg/ml (PPM). The results of analysis were validated by recovery studies. The recovery was found to be 98.75, 101 and 99.17% for three levels respectively. The % RSD for precision was found to be 0.97%. A simple, rapid, linear, accurate, precise and economical UV Spectroscopic method has been developed for estimation of Guaifenesin in tablet dosage form. The method could be considered for the determination of Guaifenesin in quality control laboratories.","PeriodicalId":7701,"journal":{"name":"American Journal of PharmTech Research","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85887217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-08DOI: 10.46624/ajptr.2019.v9.i3.028
K. Patil, T. Deshmukh, V. Patil
Please cite this article as: Patil KR et al., Stability Indicating High Performance Thin Layer Chromatographic Determination of Alogliptin Benzoate as Bulk Drug and in Tablet Dosage Form. American Journal of PharmTech Research 2019. Stability Indicating High Performance Thin Layer Chromatographic Determination of Alogliptin Benzoate as Bulk Drug and in Tablet Dosage Form K R Patil*, T A Deshmukh, V R Patil 2 1.SES Arunamai College of Pharmacy Mamurabad, Jalgaon (MS) 2.Hon. LMC College of Pharnmacy, Faizpur, Jalgaon (MS) ABSTRACT Alogliptin Benzoate is a novel hypoglycemic drug that belongs to dipeptidylpeptidase-4 inhibitor class which stimulates glucose dependent insulin release. The Present work describes development and validation of a new simple, accurate, precise and stability indicaing HPTLC method for the determination of alogliptin benzoate in tablet dosage form. The chromatographic separation was achieved by using Chloroform: Methanol 3:7 v/v as mobile phase and UV detection at 275 nm. The developed method was validated with respect to linearity, accuracy, precision, limit of detection, limit of quantitation and robustness as per ICH guidelines. The drug was subjected to stress condition of acid hydrolysis, alkali hydrolysis, photolysis, thermal degradation. Results found to be linear in concentration range of 500-2500 ng/band. The developed method can be used for the quantification of bulk drug as well as tablet dosage form
论文KR等人，稳定性指示高效薄层色谱法测定苯甲酸阿格列汀原料药和片剂剂型。美国医药技术研究杂志2019。稳定性指示高效薄层色谱法测定苯甲酸阿格列汀原料药和片剂剂型K R Patil*， T A Deshmukh, V R Patil 2 1。印度贾尔冈马穆拉巴德Arunamai药学院(MS) 2.Hon摘要苯甲酸阿格列汀是一种新型降糖药物，属于二肽基肽酶-4抑制剂类，可刺激葡萄糖依赖性胰岛素释放。建立了一种简便、准确、精密度高、稳定性好的高效液相色谱法测定苯甲酸阿格列汀片剂的含量。以三氯甲烷:甲醇3:7 v/v为流动相，在275 nm处进行紫外检测，实现了色谱分离。根据ICH指南对所开发的方法进行了线性、准确度、精密度、检出限、定量限和鲁棒性验证。药物经过酸水解、碱水解、光解、热降解等应激条件。结果表明，在500 ~ 2500 ng/波段浓度范围内呈线性关系。该方法可用于原料药和片剂剂型的定量分析
{"title":"Stability Indicating High Performance Thin Layer Chromatographic Determination of Alogliptin Benzoate as Bulk Drug and in Tablet Dosage Form","authors":"K. Patil, T. Deshmukh, V. Patil","doi":"10.46624/ajptr.2019.v9.i3.028","DOIUrl":"https://doi.org/10.46624/ajptr.2019.v9.i3.028","url":null,"abstract":"Please cite this article as: Patil KR et al., Stability Indicating High Performance Thin Layer Chromatographic Determination of Alogliptin Benzoate as Bulk Drug and in Tablet Dosage Form. American Journal of PharmTech Research 2019. Stability Indicating High Performance Thin Layer Chromatographic Determination of Alogliptin Benzoate as Bulk Drug and in Tablet Dosage Form K R Patil*, T A Deshmukh, V R Patil 2 1.SES Arunamai College of Pharmacy Mamurabad, Jalgaon (MS) 2.Hon. LMC College of Pharnmacy, Faizpur, Jalgaon (MS) ABSTRACT Alogliptin Benzoate is a novel hypoglycemic drug that belongs to dipeptidylpeptidase-4 inhibitor class which stimulates glucose dependent insulin release. The Present work describes development and validation of a new simple, accurate, precise and stability indicaing HPTLC method for the determination of alogliptin benzoate in tablet dosage form. The chromatographic separation was achieved by using Chloroform: Methanol 3:7 v/v as mobile phase and UV detection at 275 nm. The developed method was validated with respect to linearity, accuracy, precision, limit of detection, limit of quantitation and robustness as per ICH guidelines. The drug was subjected to stress condition of acid hydrolysis, alkali hydrolysis, photolysis, thermal degradation. Results found to be linear in concentration range of 500-2500 ng/band. The developed method can be used for the quantification of bulk drug as well as tablet dosage form","PeriodicalId":7701,"journal":{"name":"American Journal of PharmTech Research","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90966052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-08DOI: 10.46624/ajptr.2019.v9.i3.005
V. Krishnaraju, Haleema Fatima, Samreen Unisaa, Syed Adnan Mohiuddin Hussaini, Syed Aseem Ullah, Mohammad Maaz
Cesarean surgical site infections can be prevented by proper preoperative antibiotic prophylaxis. Differences in antibiotic selection in clinical practice exist according to clinicians preferences despite clear guidelines on preoperative antibiotic prophylaxis. The present study thus attempted to understand the antibiotic usage pattern among Lower segment Cesarian section (LSCS) patients in Hyderabad. Subjects for study were patients attending private hospitals in Hyderabad. Case records of LSCS patients who were prescribed antibiotics were used for extraction of relevant data. Other gynecological patients were excluded from the study. The mean age of LSCS patients was 24.57 years. The average number of antibiotics per patient was 2.2. The average number of other drugs per encounter was 6.45. The most commonly prescribed antibiotic was Ceftriaxone followed by Metronidazole. More than 80% of infections were treated with a combination of antimicrobials. In present study, all antibiotics were prescribed in generic name. Polypharmacy was evident from the study. All the antibiotics were given in multiple doses and the administration of antibiotics were given before incision in all patients. Considering spectrum of activity, sensitivity against resistant pathogens and less toxicity, third generation cephalosporins, especially ceftriaxone has been widely used in India for surgical prophylaxis. The common use of ceftriazone and metronidazole could be related to the possibility of mixed infections in LSCS patients. The overall antibiotic usage in LSCS patients were acceptable as per WHO “World Health Organization” standards and the general gynecological practice around the globe. Properly designed studies to access safety and efficacy of single dose vs multiple dose of antibiotics, pre and post incision antibiotic administration is need of the hour.
{"title":"Antibiotic Utilization Pattern In LSCS Patients","authors":"V. Krishnaraju, Haleema Fatima, Samreen Unisaa, Syed Adnan Mohiuddin Hussaini, Syed Aseem Ullah, Mohammad Maaz","doi":"10.46624/ajptr.2019.v9.i3.005","DOIUrl":"https://doi.org/10.46624/ajptr.2019.v9.i3.005","url":null,"abstract":"Cesarean surgical site infections can be prevented by proper preoperative antibiotic prophylaxis. Differences in antibiotic selection in clinical practice exist according to clinicians preferences despite clear guidelines on preoperative antibiotic prophylaxis. The present study thus attempted to understand the antibiotic usage pattern among Lower segment Cesarian section (LSCS) patients in Hyderabad. Subjects for study were patients attending private hospitals in Hyderabad. Case records of LSCS patients who were prescribed antibiotics were used for extraction of relevant data. Other gynecological patients were excluded from the study. The mean age of LSCS patients was 24.57 years. The average number of antibiotics per patient was 2.2. The average number of other drugs per encounter was 6.45. The most commonly prescribed antibiotic was Ceftriaxone followed by Metronidazole. More than 80% of infections were treated with a combination of antimicrobials. In present study, all antibiotics were prescribed in generic name. Polypharmacy was evident from the study. All the antibiotics were given in multiple doses and the administration of antibiotics were given before incision in all patients. Considering spectrum of activity, sensitivity against resistant pathogens and less toxicity, third generation cephalosporins, especially ceftriaxone has been widely used in India for surgical prophylaxis. The common use of ceftriazone and metronidazole could be related to the possibility of mixed infections in LSCS patients. The overall antibiotic usage in LSCS patients were acceptable as per WHO “World Health Organization” standards and the general gynecological practice around the globe. Properly designed studies to access safety and efficacy of single dose vs multiple dose of antibiotics, pre and post incision antibiotic administration is need of the hour.","PeriodicalId":7701,"journal":{"name":"American Journal of PharmTech Research","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82386165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-08DOI: 10.46624/ajptr.2019.v9.i3.010
A. N., . D. A. M., B. S., Nilakh Sayali P
{"title":"Synthesis of Biofuel from vegetable oils","authors":"A. N., . D. A. M., B. S., Nilakh Sayali P","doi":"10.46624/ajptr.2019.v9.i3.010","DOIUrl":"https://doi.org/10.46624/ajptr.2019.v9.i3.010","url":null,"abstract":"","PeriodicalId":7701,"journal":{"name":"American Journal of PharmTech Research","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89261516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-08DOI: 10.46624/ajptr.2019.v9.i3.014
Priyanka Raj G, Parthiban S, S. Gp
Priyanka Raj G*, Parthiban S, Senthil Kumar GP Dept of pharmaceutics, Bharathi College of Pharmacy, Bharathinagara, mandya, Karnataka. ABSTRACT The aim of the present investigation was to design a mucoadhesive liposomal system of Prednisone for the treatment of arthritis, severe allergic reaction multiple sclerosis that is capable of delivering entrapped drug over an extended period of time. Mucoadhesive liposomal formulations were prepared by different concentration of lecithin and cholesterol by thin film hydration technique followed by coating of liposomes by 0.2 % w/v of chitosan and Liposomes were evaluated for entrapment efficiency, particle size, zeta potential, surface morphology and invitro drug release and stability study of coated formulation. Particle size of the F4, F5 and F6 formulation was found to be 212 nm, 131 nm and 340 nm respectively and zeta potential were 164.9 mV, 165 mV and -9.6 mV, respectively. Highest entrapment efficiency was observed in the ranged of 83 % to 98% for formulation F1 ˗F8 and CF1-CF2 were 90.87 % to 94.68%. The percent drug release from F1-F8 was varied and affected by drug loading, soyalecitin and cholesterol concentration and followed non-Fickian diffusion mechanism. 2x3 factorial design were applied and studied the effect of parameter on entrapment efficiency and in vitro drug release at 2hrs, 6hrs, 12 hrs by using QI Macros R software.
{"title":"Formulation Development and Optimization by 2X3 Factorial Design of Novel Prednisone Loaded Mucoadhesive Liposomal Formulation","authors":"Priyanka Raj G, Parthiban S, S. Gp","doi":"10.46624/ajptr.2019.v9.i3.014","DOIUrl":"https://doi.org/10.46624/ajptr.2019.v9.i3.014","url":null,"abstract":"Priyanka Raj G*, Parthiban S, Senthil Kumar GP Dept of pharmaceutics, Bharathi College of Pharmacy, Bharathinagara, mandya, Karnataka. ABSTRACT The aim of the present investigation was to design a mucoadhesive liposomal system of Prednisone for the treatment of arthritis, severe allergic reaction multiple sclerosis that is capable of delivering entrapped drug over an extended period of time. Mucoadhesive liposomal formulations were prepared by different concentration of lecithin and cholesterol by thin film hydration technique followed by coating of liposomes by 0.2 % w/v of chitosan and Liposomes were evaluated for entrapment efficiency, particle size, zeta potential, surface morphology and invitro drug release and stability study of coated formulation. Particle size of the F4, F5 and F6 formulation was found to be 212 nm, 131 nm and 340 nm respectively and zeta potential were 164.9 mV, 165 mV and -9.6 mV, respectively. Highest entrapment efficiency was observed in the ranged of 83 % to 98% for formulation F1 ˗F8 and CF1-CF2 were 90.87 % to 94.68%. The percent drug release from F1-F8 was varied and affected by drug loading, soyalecitin and cholesterol concentration and followed non-Fickian diffusion mechanism. 2x3 factorial design were applied and studied the effect of parameter on entrapment efficiency and in vitro drug release at 2hrs, 6hrs, 12 hrs by using QI Macros R software.","PeriodicalId":7701,"journal":{"name":"American Journal of PharmTech Research","volume":"80 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77446239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-08DOI: 10.46624/ajptr.2019.v9.i3.008
M. Lakshmikanth, B. Rajkamal
Please cite this article as: Kanth LM et al., RP-HPLC Method Development and Validation for Estimation of Lenvatinib In Bulk and Pharmaceutical Dosage Form. American Journal of PharmTech Research 2019. RP-HPLC Method Development and Validation for Estimation of Lenvatinib In Bulk and Pharmaceutical Dosage Form Lakshmi Kanth M*, Raj Kamal B 1.Research Scholar, Mewar University, Chittorgarh, Rajasthan, India. 2.Research Supervisor, Mewar University, Chittorgarh, Rajasthan, India ABSTRACT The objective of present work was to develop and validate a rapid reverse phase high-performance liquid chromatography (RP-HPLC) method for the quantitative analysis of lenvatinib in bulk and pharmaceutical dosage forms. Chromatographic analyses were performed on an ODC column of 250mm 4.6mm: i.d and 5μ particle size with a mobile phase comprising of 0.5M ammonium acetate and acetonitrile in the ratio 90:10 v/v. The flow rate maintained at 1 ml/min, detected lenvatinib at RT 1.15 minutes. The lenvatinib was detected and quantitated using a photodiode array detector at a wavelength of 367 nm. The method was shown to be specific and linear in the range of 20-120μg/ml (r= 0.999). The precision (intraand inter-day) was demonstrated. The method is robust relative to changes in flow rate, column and temperature. The limits of detection and quantitation were 0.4 and 0.12μg/ml respectively. Validation parameters such as specificity, linearity, precision, accuracy, and robustness, limit of detection (LOD) and limit of quantitation (LOQ) were evaluated for the method according to the International Conference on Harmonization (ICH) Q2 R1 guidelines. The method fulfilled the requirements for reliability and feasibility for application to the quantitative analysis of lenvatinib in bulk and pharmaceutical dosage forms.
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