Pub Date : 2021-01-01DOI: 10.5352/JLS.2021.31.1.83
H. Oh, Hye-Jin Go, N. Park
Uu-ilys, an i-type lysozyme from spoon worm (Urechis unicinctus), is an innate immune factor that plays an important role in the defense against pathogens. It also possesses non-enzymatic antibacterial activity. Thus, there is a possibility to develop an antimicrobial model peptide from Uu-ilys. In this study, we report the design, production, and antibacterial activity of an Uu-ilys analog that exhibits antibacterial activity. The Uu-ilys structure was fragmented according to its secondary structures to predict the regions with antimicrobial activity using antimicrobial peptide (AMP) prediction tools from different AMP databases. A peptide containing the C-terminal fragment was predicted to exert antimicrobial activity. The chosen fragment was designated as an Uu-ilys analog containing the C-terminal fragment, Uuilys-CF. To examine the possibility of developing an AMP using the sequence of Uu-ilys-CF, recombinant fusion protein (TrxA-Uu-ilys-CF) was produced in an expression system that was heterologous. The produced fusion protein was cleaved after methionine leaving Uu-ilys-CF free from the fusion protein. This was then isolated through high performance liquid chromatography and reverse phase column, CapCell-Pak C18. The antibacterial activity of Uu-ilys-CF against different microbial strains (four gram-positive, six gram-negative, and one fungal strain) were assessed through the ultrasensitive radial diffusion assay (URDA). Among the bacterial strains tested, Salmonella enterica was the most susceptible. While the fungal strain tested was not susceptible to Uu-ilys-CF, broad spectrum antibacterial activity was observed.
{"title":"Recombinant Production and Antimicrobial Activity of an Antimicrobial Model Peptide (Uu-ilys-CF) Derived from Spoon Worm Lysozyme, Uu-ilys","authors":"H. Oh, Hye-Jin Go, N. Park","doi":"10.5352/JLS.2021.31.1.83","DOIUrl":"https://doi.org/10.5352/JLS.2021.31.1.83","url":null,"abstract":"Uu-ilys, an i-type lysozyme from spoon worm (Urechis unicinctus), is an innate immune factor that plays an important role in the defense against pathogens. It also possesses non-enzymatic antibacterial activity. Thus, there is a possibility to develop an antimicrobial model peptide from Uu-ilys. In this study, we report the design, production, and antibacterial activity of an Uu-ilys analog that exhibits antibacterial activity. The Uu-ilys structure was fragmented according to its secondary structures to predict the regions with antimicrobial activity using antimicrobial peptide (AMP) prediction tools from different AMP databases. A peptide containing the C-terminal fragment was predicted to exert antimicrobial activity. The chosen fragment was designated as an Uu-ilys analog containing the C-terminal fragment, Uuilys-CF. To examine the possibility of developing an AMP using the sequence of Uu-ilys-CF, recombinant fusion protein (TrxA-Uu-ilys-CF) was produced in an expression system that was heterologous. The produced fusion protein was cleaved after methionine leaving Uu-ilys-CF free from the fusion protein. This was then isolated through high performance liquid chromatography and reverse phase column, CapCell-Pak C18. The antibacterial activity of Uu-ilys-CF against different microbial strains (four gram-positive, six gram-negative, and one fungal strain) were assessed through the ultrasensitive radial diffusion assay (URDA). Among the bacterial strains tested, Salmonella enterica was the most susceptible. While the fungal strain tested was not susceptible to Uu-ilys-CF, broad spectrum antibacterial activity was observed.","PeriodicalId":7759,"journal":{"name":"American Journal of Life Sciences","volume":"2 1","pages":"83-89"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87443213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.5352/JLS.2021.31.4.389
J. Ji, Y. Nam, Sang-mo Kang
{"title":"Centella asiatica and Asiaticoside Regulate H 2 O 2 -induced Cellular Inflammation via Mitochondrial Respiration and the TLR4 Pathway","authors":"J. Ji, Y. Nam, Sang-mo Kang","doi":"10.5352/JLS.2021.31.4.389","DOIUrl":"https://doi.org/10.5352/JLS.2021.31.4.389","url":null,"abstract":"","PeriodicalId":7759,"journal":{"name":"American Journal of Life Sciences","volume":"7 1","pages":"389-399"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85422288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.5352/JLS.2021.31.5.520
L. Mamuad, Sang-Suk Lee
{"title":"The Role of Glutamic Acid-producing Microorganisms in Rumen Microbial Ecosystems","authors":"L. Mamuad, Sang-Suk Lee","doi":"10.5352/JLS.2021.31.5.520","DOIUrl":"https://doi.org/10.5352/JLS.2021.31.5.520","url":null,"abstract":"","PeriodicalId":7759,"journal":{"name":"American Journal of Life Sciences","volume":"1 1","pages":"520-526"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80395394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.11648/j.ajls.20210904.11
Alemu Ayele Zerihun
{"title":"Evaluation of Ethiopian Bread Wheat Cultivars for Slow Rusting Resistance to Yellow Rust (Puccinia striiformis f.s Tritici)","authors":"Alemu Ayele Zerihun","doi":"10.11648/j.ajls.20210904.11","DOIUrl":"https://doi.org/10.11648/j.ajls.20210904.11","url":null,"abstract":"","PeriodicalId":7759,"journal":{"name":"American Journal of Life Sciences","volume":"145 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86020844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.11648/j.ajls.20210905.16
Lijun Mu, E. Xie, Xinwei Zhang, Juanhua Tian, Meiyu Wang, Dapeng Wu, Yue-Feng Du
: OnabotulinumtoxinA therapy has become widely used in overactive bladder syndrome (OAB), and many relevant articles have been published, however, there is no consensus regarding the clinical effect of onabotulinumtoxinA with the different dosages. Therefore, we conducted this meta-analysis to assess the efficacy and safety of 100 U, 200 U and 300 U onabotulinumtoxinA for the treatment of (OAB). In this project, We performed a comprehensive literature search, which was performed using EMBASE, PubMed, Cochrane database, and Google Scholar for randomized controlled trials (from inception to February 2017). As a result, fourteen studies with 1999 participants were selected. For the efficacy, 200 U of OnabotulinumtoxinA was significantly superior to 100 U, especially in the maximum cystometric capacity (MCC) and maximum detrusor pressure (MDP). Also, its subjective cure rate showed the same tendency. There were no statistical differences between 200 U and 300 U in MCC, MDP and subjective cure rate. For the adverse events, there were no statistical differences among 100 U, 200 U and 300 U OnabotulinumtoxinA in urinary tract infection (UTI) and urinary retention. Therefore, in our study. Compared to 100 U OnabotulinumtoxinA, 200 U OnabotulinumtoxinA has better efficacy while maintaining safety. Although 200 U OnabotulinumtoxinA is comparable to 300 U OnabotulinumtoxinA in terms of safety and efficacy, 200 U OnabotulinumtoxinA is a cost-effective intervention and may appear to be the optimal dosage for OAB populations.
OnabotulinumtoxinA治疗膀胱过度活动综合征(OAB)已被广泛应用，相关文章已发表，但不同剂量OnabotulinumtoxinA的临床疗效尚未达成共识。因此，我们进行了这项荟萃分析，以评估100u、200u和300u肉毒杆菌毒素a治疗OAB的有效性和安全性。在这个项目中，我们进行了全面的文献检索，使用EMBASE、PubMed、Cochrane数据库和Google Scholar进行随机对照试验(从成立到2017年2月)。结果，有14项研究，1999名参与者被选中。在疗效方面，200 U的OnabotulinumtoxinA显著优于100 U，特别是在最大膀胱容量(MCC)和最大逼尿肌压力(MDP)方面。主观治愈率也呈现出相同的趋势。在MCC、MDP及主观治愈率方面，200u与300u组比较无统计学差异。在不良事件方面，100 U、200 U和300 U的肉毒杆菌毒素组尿路感染(UTI)和尿潴留发生率无统计学差异。因此，在我们的研究中。与100 U OnabotulinumtoxinA相比，200 U OnabotulinumtoxinA在保持安全性的同时具有更好的疗效。尽管在安全性和有效性方面，200 U的OnabotulinumtoxinA与300 U的onabotulintoxina相当，但200 U的onabotulintoxina是一种具有成本效益的干预措施，可能是OAB人群的最佳剂量。
{"title":"The Efficacy and Safety of OnabotulinumtoxinA with Different Dosages for the Treatment of Overactive Bladder Syndrome: A Systematic Review and Meta-analysis","authors":"Lijun Mu, E. Xie, Xinwei Zhang, Juanhua Tian, Meiyu Wang, Dapeng Wu, Yue-Feng Du","doi":"10.11648/j.ajls.20210905.16","DOIUrl":"https://doi.org/10.11648/j.ajls.20210905.16","url":null,"abstract":": OnabotulinumtoxinA therapy has become widely used in overactive bladder syndrome (OAB), and many relevant articles have been published, however, there is no consensus regarding the clinical effect of onabotulinumtoxinA with the different dosages. Therefore, we conducted this meta-analysis to assess the efficacy and safety of 100 U, 200 U and 300 U onabotulinumtoxinA for the treatment of (OAB). In this project, We performed a comprehensive literature search, which was performed using EMBASE, PubMed, Cochrane database, and Google Scholar for randomized controlled trials (from inception to February 2017). As a result, fourteen studies with 1999 participants were selected. For the efficacy, 200 U of OnabotulinumtoxinA was significantly superior to 100 U, especially in the maximum cystometric capacity (MCC) and maximum detrusor pressure (MDP). Also, its subjective cure rate showed the same tendency. There were no statistical differences between 200 U and 300 U in MCC, MDP and subjective cure rate. For the adverse events, there were no statistical differences among 100 U, 200 U and 300 U OnabotulinumtoxinA in urinary tract infection (UTI) and urinary retention. Therefore, in our study. Compared to 100 U OnabotulinumtoxinA, 200 U OnabotulinumtoxinA has better efficacy while maintaining safety. Although 200 U OnabotulinumtoxinA is comparable to 300 U OnabotulinumtoxinA in terms of safety and efficacy, 200 U OnabotulinumtoxinA is a cost-effective intervention and may appear to be the optimal dosage for OAB populations.","PeriodicalId":7759,"journal":{"name":"American Journal of Life Sciences","volume":"80 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84117672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.5352/JLS.2021.31.3.287
Hyun Hwangbo, Ji-Suk Jeung, Min Young Kim, S. Ji, Yoon Seonhye, Tae hee Kim, S. O. Kim, Yung-Hyun Choi
{"title":"A Study on Antioxidant and Anti-inflammatory Effects Based on Analysis of Functional Components of Cornus officinalis Siebold & Zucc.","authors":"Hyun Hwangbo, Ji-Suk Jeung, Min Young Kim, S. Ji, Yoon Seonhye, Tae hee Kim, S. O. Kim, Yung-Hyun Choi","doi":"10.5352/JLS.2021.31.3.287","DOIUrl":"https://doi.org/10.5352/JLS.2021.31.3.287","url":null,"abstract":"","PeriodicalId":7759,"journal":{"name":"American Journal of Life Sciences","volume":"9 1","pages":"287-297"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88765941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.11648/j.ajls.20210905.15
Han Tuo, Gong Hong, M. Fatima, L. Ying, Fan Yajie, S. Wei, Yao Zhihui, Wang Qian, Wang Congxia
Background: Cardiovascular disease (CVD) is the lead cause of death around the world. Dyslipidemia is strongly associated with CVD. Magnesium (Mg) is an essential trace element involved in many vital biological process, such as glucose and lipid metabolism. Previous studies have showed that hypomagnesemia or Mg deficiency is associated with an increased risk of type 2 diabetes, metabolic syndrome, hypertension, and CVD. Objective: To explore the correlation between serum magnesium (Mg) concentration and dyslipidemia in health subjects and type 2 diabetes patients. Methods: 3430 health subjects were selected and divided into adult (aged 18~59) and elderly (≥60 years) groups according to age. In addition, 63 patients with type 2 diabetes were selected. Propensity score based on age, gender, body mass index (BMI) and smoking history was matched at a ratio of 1:2 between patients and health controls. Results: There was a significant correlation between elevated serum Mg concentration and dyslipidemia in health adults, but no significant correlation between the two in the health elderly. Multiple linear regression showed that serum total cholesterol (TC), BMI and smoking in men while fasting plasma glucose and serum LDL-C in women were independent risk factors for elevated serum Mg concentration. Whereas, no significant correlation was found between serum Mg concentration and lipid levels in T2DM patients. Conclusion: Serum Mg concentration is correlated with serum lipids to a certain extent, while age, gender and disease status may also exert certain confound effects. The causal relationship between serum Mg and dyslipidemia needs to be further studied.
{"title":"Correlation Analysis of Serum Magnesium Concentration and Lipids in Health Population and Type 2 Diabetes Patients","authors":"Han Tuo, Gong Hong, M. Fatima, L. Ying, Fan Yajie, S. Wei, Yao Zhihui, Wang Qian, Wang Congxia","doi":"10.11648/j.ajls.20210905.15","DOIUrl":"https://doi.org/10.11648/j.ajls.20210905.15","url":null,"abstract":"Background: Cardiovascular disease (CVD) is the lead cause of death around the world. Dyslipidemia is strongly associated with CVD. Magnesium (Mg) is an essential trace element involved in many vital biological process, such as glucose and lipid metabolism. Previous studies have showed that hypomagnesemia or Mg deficiency is associated with an increased risk of type 2 diabetes, metabolic syndrome, hypertension, and CVD. Objective: To explore the correlation between serum magnesium (Mg) concentration and dyslipidemia in health subjects and type 2 diabetes patients. Methods: 3430 health subjects were selected and divided into adult (aged 18~59) and elderly (≥60 years) groups according to age. In addition, 63 patients with type 2 diabetes were selected. Propensity score based on age, gender, body mass index (BMI) and smoking history was matched at a ratio of 1:2 between patients and health controls. Results: There was a significant correlation between elevated serum Mg concentration and dyslipidemia in health adults, but no significant correlation between the two in the health elderly. Multiple linear regression showed that serum total cholesterol (TC), BMI and smoking in men while fasting plasma glucose and serum LDL-C in women were independent risk factors for elevated serum Mg concentration. Whereas, no significant correlation was found between serum Mg concentration and lipid levels in T2DM patients. Conclusion: Serum Mg concentration is correlated with serum lipids to a certain extent, while age, gender and disease status may also exert certain confound effects. The causal relationship between serum Mg and dyslipidemia needs to be further studied.","PeriodicalId":7759,"journal":{"name":"American Journal of Life Sciences","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75559600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.5352/JLS.2021.31.3.280
Park Ho-Yeon, Ahn Dong-Gyu, Kim Soon-Young
{"title":"Effect of Inhibitors of Ethylene Production on Growth and Gravitropism Inhibited by Oryzalin in Arabidopsis Roots","authors":"Park Ho-Yeon, Ahn Dong-Gyu, Kim Soon-Young","doi":"10.5352/JLS.2021.31.3.280","DOIUrl":"https://doi.org/10.5352/JLS.2021.31.3.280","url":null,"abstract":"","PeriodicalId":7759,"journal":{"name":"American Journal of Life Sciences","volume":"25 1","pages":"280-286"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80182003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.5352/JLS.2021.31.7.677
H. Sohn, MunJung Kim, Young-Min Kim
Cannabis sativa L. belongs to the Cannabaceae family and is an annual herbaceous flowing plant. The plants can be classified into narcotic marijuana and nonnarcotic hemp. Different parts of C. sativa L. have been used as food, medicine, cosmetics, fiber and textile. However, the use of leaf, flower, and seed of C. sativa L was forbidden in Korea in January 1977 as a result of the Cannabis Control Act due to the narcotic properties. The plant’s mature stems have limited uses for the production of fiber and sheets. Recently, various cannabinoids, terpenes and essential fatty acids were identified from C. sativa L., and their safety and useful bio-activities, such as neuroprotective, anti-inflammation, antithrombosis, antiepileptic, and antimicrobial activities, and the relief of pain, have been highlighted. Furthermore, the process of reduction of tetrahydrocannabinol, a representative narcotic compound, and the isolation of cannabidiol, a nonnarcotic active compound in C. sativa L., have been determined. These findings resulted in the legalization of C. sativa L. in Korea for medical use in December 2018 and the exclusion of C. sativa L. from the narcotic list of the UN Commission on Narcotics Drugs (UNCND) in December 2020. Therefore, developments of various high-value added products have commenced worldwide. Additionally, in 2021, the Korean government deregulated special zones based on hemp. In this study, the current status and the prospect of the hemp industry, as well as essential techniques for developing new hemp products, are provided for the activation of the Korea Green-Rush.
{"title":"Current Status and Prospects for the Hemp Bioindustry","authors":"H. Sohn, MunJung Kim, Young-Min Kim","doi":"10.5352/JLS.2021.31.7.677","DOIUrl":"https://doi.org/10.5352/JLS.2021.31.7.677","url":null,"abstract":"Cannabis sativa L. belongs to the Cannabaceae family and is an annual herbaceous flowing plant. The plants can be classified into narcotic marijuana and nonnarcotic hemp. Different parts of C. sativa L. have been used as food, medicine, cosmetics, fiber and textile. However, the use of leaf, flower, and seed of C. sativa L was forbidden in Korea in January 1977 as a result of the Cannabis Control Act due to the narcotic properties. The plant’s mature stems have limited uses for the production of fiber and sheets. Recently, various cannabinoids, terpenes and essential fatty acids were identified from C. sativa L., and their safety and useful bio-activities, such as neuroprotective, anti-inflammation, antithrombosis, antiepileptic, and antimicrobial activities, and the relief of pain, have been highlighted. Furthermore, the process of reduction of tetrahydrocannabinol, a representative narcotic compound, and the isolation of cannabidiol, a nonnarcotic active compound in C. sativa L., have been determined. These findings resulted in the legalization of C. sativa L. in Korea for medical use in December 2018 and the exclusion of C. sativa L. from the narcotic list of the UN Commission on Narcotics Drugs (UNCND) in December 2020. Therefore, developments of various high-value added products have commenced worldwide. Additionally, in 2021, the Korean government deregulated special zones based on hemp. In this study, the current status and the prospect of the hemp industry, as well as essential techniques for developing new hemp products, are provided for the activation of the Korea Green-Rush.","PeriodicalId":7759,"journal":{"name":"American Journal of Life Sciences","volume":"32 1","pages":"677-685"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89773213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.5352/JLS.2021.31.5.481
Ji-Eun Lee, Xiaotong Xu, S. Jeong, W. Kang, S. Ryu, Yehum Kim, D. Ahn
The purpose of this study was to identify fungi that degrade product value during the storage and distribution of confectionery products, and to investigate the antifungal effect of organic acid and UV-C treatments on high carbohydrate products. Fungi isolated from spoiled high carbohydrate confectionery were identified as Wallemia sp., Aspergillus sp-1 and Aspergillus sp-2 depending on homologies with ITS1 and ITS4 sequences. The isolated fungi were assayed for antifungal activity by treatment with acetic acid, citric acid, lactic acid or maleic acid. As a result, it was confirmed that the growth of Wallemia sp. and Aspergillus sp-2 was suppressed by treatment with 0.2 M and 0.35 M acetic acid, respectively. In addition, as a result of confirming the antifungal effect according to the UV-C irradiation time, the growth inhibitory effects of Wallemia sp. and Aspergillus sp-2 were shown in irradiation for 30 min and the growth inhibitory effect of Aspergillus sp-1 was shown in irradiation for 40 min. The result of the sensory evaluation of the untreated and 0.35 M acetic acid-treated high carbohydrate confectionery, there were not significant changes in taste, color, abnormal taste, hardness and texture, but there were significant differences in sour taste and smell. As a result of the above study, the effect of inhibiting fungi growth on the product by treatment with organic acid and UV-C irradiation was confirmed, and it is expected to be used in confectionery that were concerned about the occurrence of fungi in the distribution process.
{"title":"Effect of Ultraviolet-C and Organic Acid Treatment on Fungi Isolated from High Carbohydrate Confectionery","authors":"Ji-Eun Lee, Xiaotong Xu, S. Jeong, W. Kang, S. Ryu, Yehum Kim, D. Ahn","doi":"10.5352/JLS.2021.31.5.481","DOIUrl":"https://doi.org/10.5352/JLS.2021.31.5.481","url":null,"abstract":"The purpose of this study was to identify fungi that degrade product value during the storage and distribution of confectionery products, and to investigate the antifungal effect of organic acid and UV-C treatments on high carbohydrate products. Fungi isolated from spoiled high carbohydrate confectionery were identified as Wallemia sp., Aspergillus sp-1 and Aspergillus sp-2 depending on homologies with ITS1 and ITS4 sequences. The isolated fungi were assayed for antifungal activity by treatment with acetic acid, citric acid, lactic acid or maleic acid. As a result, it was confirmed that the growth of Wallemia sp. and Aspergillus sp-2 was suppressed by treatment with 0.2 M and 0.35 M acetic acid, respectively. In addition, as a result of confirming the antifungal effect according to the UV-C irradiation time, the growth inhibitory effects of Wallemia sp. and Aspergillus sp-2 were shown in irradiation for 30 min and the growth inhibitory effect of Aspergillus sp-1 was shown in irradiation for 40 min. The result of the sensory evaluation of the untreated and 0.35 M acetic acid-treated high carbohydrate confectionery, there were not significant changes in taste, color, abnormal taste, hardness and texture, but there were significant differences in sour taste and smell. As a result of the above study, the effect of inhibiting fungi growth on the product by treatment with organic acid and UV-C irradiation was confirmed, and it is expected to be used in confectionery that were concerned about the occurrence of fungi in the distribution process.","PeriodicalId":7759,"journal":{"name":"American Journal of Life Sciences","volume":"179 1","pages":"481-487"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89069999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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