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Techno-economic analysis of bioplastic and biofuel production from a high-ash microalgae biofilm cultivated in effluent from a municipal anaerobic digester 利用市政厌氧发酵池污水中培养的高灰分微藻生物膜生产生物塑料和生物燃料的技术经济分析
IF 4.6 2区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-10-26 DOI: 10.1016/j.algal.2024.103774
Rotating Algae Biofilm Reactors (RABRs) are a promising technology for efficient treatment of wastewater and production of algae-based bioproducts. However, RABR-grown algae can contain a high content of ash (30–60 wt%, dry basis), which influences the technical and economic feasibility of bioproduct conversion processes. In this report, experimental studies and economic analysis were conducted to compare different processes for bioproduct conversion of a high-ash microalgae biofilm grown using a RABR treating 0.6 million gallons per day of anaerobic digestion centrate at the Central Valley Water Reclamation Facility in Salt Lake City, UT. Process and economic models were developed and compared for three conversion processes: 1) the production of bioplastics, 2) the production of bioplastics with a lipid-extraction pretreatment, and 3) the production of biocrude via hydrothermal liquefaction. Techno-economic analysis was performed for each conversion process, including three cases for algae productivity: 231, 391, and 577 metric tons per year (dry basis). The calculated value for the minimum plastic selling price (MPSP) of bioplastics produced from algae ranges from $4050 to $3520 per metric ton based on the baseline and final productivity cases of the RABR, respectively. The extraction of lipids in addition to bioplastic production results in an MPSP of $4570 to $4000 per metric ton for the same productivity cases. The relatively small production scale and complex processing for hydrothermal liquefaction results in a minimum fuel selling price of the biocrude of $5.32 per gallon of gasoline equivalent. The conversion process for bioplastic production from whole algae has the highest income:expense ratio and the most cost-competitive pricing of the three modeled processes.
旋转藻类生物膜反应器(RABRs)是一种很有前途的技术,可用于高效处理废水和生产藻类生物产品。然而,RABR 生长的藻类可能含有较高的灰分(30-60 wt%,干基),这影响了生物产品转化过程的技术和经济可行性。在本报告中,我们进行了实验研究和经济分析,比较了在犹他州盐湖城中央谷水回收设施中使用 RABR(每天处理 60 万加仑厌氧消化中心液)生长的高灰分微藻生物膜进行生物产品转化的不同工艺。针对三种转换工艺开发并比较了工艺和经济模型:1) 生产生物塑料;2) 通过脂质提取预处理生产生物塑料;3) 通过水热液化生产生物原油。对每种转化工艺都进行了技术经济分析,包括藻类生产力的三种情况:每年 231、391 和 577 公吨(干基)。根据 RABR 的基准和最终生产率情况,藻类生产的生物塑料的最低塑料销售价格(MPSP)计算值分别为每公吨 4050 美元至 3520 美元。在生产率相同的情况下,除生产生物塑料外还提取脂类,则每公吨的 MPSP 为 4570 美元至 4000 美元。由于生产规模相对较小,水热液化工艺复杂,生物原油的最低燃料售价为每加仑汽油当量 5.32 美元。在三种模拟工艺中,利用整株藻类生产生物塑料的转化工艺具有最高的收支比和最具成本竞争力的定价。
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引用次数: 0
The growth and nitrogen utilization strategies in two dominant Symbiodiniaceae species facing nitrogen deficiency and enrichment 面临氮缺乏和氮富集的两种优势共生草本植物的生长和氮利用策略
IF 4.6 2区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-10-24 DOI: 10.1016/j.algal.2024.103772
Symbiodiniaceae, a diverse group of unicellular dinoflagellates, are well known as endosymbionts of marine invertebrates (e.g., corals, giant clams and foraminiferans). Currently, how in vitro cultured Symbiodiniaceae cope with nitrogen (N) deficiency and enhancement remain largely unexplored. To address this knowledge gap, this study investigated the dynamics of growth, photosynthesis and crystalline guanine formation in response to differential N supplies in two dominant Symbiodiniaceae species: Durusdinium trenchii and Cladocopium goreaui. The results indicated growth rate and photosynthesis were closely related to N sources and concentrations. Under N deficiency, cell growth was inhibited. As N concentration increased, both D. trenchii and C. goreaui exhibited flexible strategies for utilizing different N sources. When provided with nitrate (NO3), C. goreaui and D. trenchii showed an enhancement in the algal growth and photosynthetic efficiency (Fv/Fm). When ammonium (NH4+) was supplied, a moderate increase of NH4+ can benefit cell growth and photosynthesis, but excessive enrichment adversely affected algal growth. Additionally, Raman microscopy demonstrated that cellular crystalline guanine was formed by C. goreaui when exposed to N supply, but gradually decreased as N was consumed in medium. A similar phenomenon was observed in D. trenchii. We proposed that crystalline guanine may serve as an important N storage and utilization strategy. This study delved into the growth strategies and adaptability of Symbiodiniaceae to varying N nutritional environments, which contributes to understanding the symbiotic relationship between Symbiodiniaceae and corals.
共生藻科(Symbiodiniaceae)是一类种类繁多的单细胞甲藻,是众所周知的海洋无脊椎动物(如珊瑚、巨蛤和有孔虫)的内共生体。目前,体外培养的共生藻如何应对氮(N)缺乏和增加的问题在很大程度上仍未得到研究。为了填补这一知识空白,本研究调查了两种优势共生藤本植物在不同氮供应条件下的生长、光合作用和结晶鸟嘌呤形成动态:Durusdinium trenchii 和 Cladocopium goreaui。结果表明,生长速度和光合作用与氮源和氮浓度密切相关。缺氮时,细胞生长受到抑制。随着氮浓度的增加,D. trenchii 和 C. goreaui 都表现出灵活利用不同氮源的策略。当提供硝酸盐(NO3-)时,C. goreaui 和 D. trenchii 的藻类生长和光合效率(Fv/Fm)都有所提高。当提供铵(NH4+)时,适度增加 NH4+ 有利于细胞生长和光合作用,但过度富集会对藻类生长产生不利影响。此外,拉曼显微镜研究表明,高丽藻在氮供应条件下会形成细胞结晶鸟嘌呤,但随着培养基中氮的消耗,结晶鸟嘌呤逐渐减少。在 D. trenchii 中也观察到了类似的现象。我们认为结晶鸟嘌呤可能是一种重要的氮储存和利用策略。本研究深入探讨了共生苔藓植物的生长策略和对不同氮营养环境的适应性,有助于了解共生苔藓植物与珊瑚之间的共生关系。
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引用次数: 0
The effects of influent 5-Deoxystrigol concentrations on integral biogas upgrading and nutrient removal by different algal-fungal-bacterial consortium 进水 5-脱氧斯的明浓度对不同藻类-真菌-细菌联合体整体沼气升级和营养物质去除的影响
IF 4.6 2区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-10-23 DOI: 10.1016/j.algal.2024.103770
The low quality of biogas and the difficulties in treating biogas slurry are important bottlenecks that limit the development of the fermentation industry. The effects of 5-deoxystrigol (5DS) on the growth rate, daily productivity, and photosynthetic performance of different algal-fungal-bacterial consortia were investigated, and the auxiliary activities of endophytic bacteria (S395–2), Ganoderma lucidum (G. lucidum), Bacillus licheniformis (B. licheniformis), and activated sludge in these systems were examined. The consortium Chlorella vulgaris (C. vulgaris+ G. lucidum + S395–2 was found to be the most effective combination for both biogas upgrading and nutrient removal. Removal efficiencies of chemical oxygen demand (COD), total nitrogen (TN), and total phosphorous (TP) were approximately 82.94 ± 7.88 %, 81.36 ± 7.79 %, and 83.27 ± 8.09 %, respectively, and the CO2 removal efficiency was observed to be about 69.89 ± 6.57 % at the optimum 5DS concentration of 10−11 M. On day 7 of the treatment, the CH4 content was elevated from 66.07 ± 4.84 % to 86.24 ± 8.44 %. The effect of 5DS treatments in different algal-fungal-bacterial consortia was positive in terms of growth performance and photosynthetic rate. The present study provides a framework for efficient biogas upgrading and nutrient removal by the three-phase symbionts.
沼气质量不高、沼液处理困难是制约发酵工业发展的重要瓶颈。研究了5-脱氧斯的明(5DS)对不同藻类-真菌-细菌联合体的生长速率、日生产力和光合作用性能的影响,并考察了内生菌(S395-2)、灵芝(G. lucidum)、地衣芽孢杆菌(B. licheniformis)和活性污泥在这些系统中的辅助活性。结果发现,小球藻(C. vulgaris)+灵芝(G. lucidum)+S395-2 联合体是沼气升级和营养物质去除的最有效组合。在最佳 5DS 浓度为 10-11 M 时,化学需氧量 (COD)、总氮 (TN) 和总磷 (TP) 的去除率分别约为 82.94 ± 7.88 %、81.36 ± 7.79 % 和 83.27 ± 8.09 %,二氧化碳的去除率约为 69.89 ± 6.57 %。在不同的藻类-真菌-细菌联合体中,5DS 处理对生长性能和光合速率的影响是积极的。本研究为三相共生体的高效沼气升级和养分去除提供了一个框架。
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引用次数: 0
Growth and biochemical composition of green seaweed Chaetomorpha linum (O.F. Müller) Kützing (1845) cultured in a range of ionic profiles 在一定离子浓度范围内培养的绿藻 Chaetomorpha linum (O.F. Müller) Kützing (1845) 的生长和生化成分
IF 4.6 2区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-10-22 DOI: 10.1016/j.algal.2024.103769
Seaweed aquaculture in Inland Saline Water (ISW) can utilise degraded land to produce valuable products. ISW is characterized by its different ionic profile to ocean water (OW), particularly the ratios of sodium (Na+) to potassium (K+) ions, and magnesium (Mg2+) to calcium (Ca2+) ions. The feasibility of seaweed cultivation in varying ionic profiles is not yet well understood. This study investigates the growth of Chaetomorpha linum by length (SL) and biomass (TB), cultured in several ionic profiles by mixture of ISW with OW over two 15 day experiments. The first experiment (EXP 1) investigated growth across a broad range of ionic profiles. C. linum was then cultivated across a more specific range of profiles in the second experiment (EXP 2), based on EXP 1 results. Tanks (50 L) were randomly organised outdoors and salinity was maintained at approximately 25 ppt. SL and TB of seaweed was recorded every 3 days. The proximate and mineral composition of harvested seaweed on Day 15 was also analysed. A significant negative correlation (p < 0.001, r = −0.835, n = 13) was observed between non-discrete TB specific growth rate (SGR) and increasing K+ of media in EXP 1. A significant positive correlation (p < 0.001, r = 0.769, n = 28, EXP 1) was observed between potassium (K) in dry matter (mg.g−1) and K+ in media, suggesting lower K+ in media causes increased TB due to higher water flow rate into cells. Calcium (Ca) content (% d.b.) was much higher in K+ deficient media, indicating Ca accumulation occurs in cells during ion homeostasis when K is not available. Positive SL SGR's across ionic profiles also indicate this species is capable of a high level of internal ion regulation. This study provides context for commercial cultivation of C. linum in varying water types, and presents an avenue for salinity-affected dryland farmers to diversify their income through ISW aquaculture.
内陆盐水(ISW)中的海藻养殖可利用退化土地生产有价值的产品。内陆盐水(ISW)的特点是其离子状况与海水(OW)不同,特别是钠离子(Na+)与钾离子(K+)的比例,以及镁离子(Mg2+)与钙离子(Ca2+)的比例。在不同离子状况下栽培海藻的可行性尚不十分清楚。本研究通过两个为期 15 天的实验,研究了在多种离子状况下通过混合 ISW 和 OW 培养的 Chaetomorpha linum 的长度(SL)和生物量(TB)的生长情况。第一个实验(EXP 1)研究了多种离子状况下的生长情况。根据 EXP 1 的结果,在第二次实验(EXP 2)中,亚麻酸藻在更具体的离子浓度范围内生长。水槽(50 升)在室外随机布置,盐度保持在约 25 ppt。每 3 天记录一次海藻的盐度和盐浓度。还分析了第 15 天收获的海藻的近似物和矿物质成分。在 EXP 1 中,非离散 TB 特定生长率(SGR)与培养基中 K+ 的增加之间存在明显的负相关(p < 0.001,r = -0.835,n = 13)。 在干物质中的钾(K)含量(mg.g-1)与培养基中的 K+ 之间存在明显的正相关(p < 0.001,r = 0.769,n = 28,EXP 1),这表明培养基中的 K+ 越低,进入细胞的水流量越大,从而导致 TB 增加。钙(Ca)含量(% d.b.)在 K+ 缺乏的培养基中要高得多,这表明当 K 缺乏时,细胞中的钙会在离子平衡过程中积累。不同离子状况下的正 SL SGR 也表明该物种能够进行高水平的内部离子调节。这项研究为在不同水质中商业化养殖亚麻藻提供了背景,并为受盐碱影响的旱地农民提供了一条通过ISW水产养殖实现收入多样化的途径。
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引用次数: 0
A comparative analysis on impact of drying methods on antioxidants, antidiabetes and antiobesity activities in green algae Caulerpa lentillifera: In vitro study 比较分析干燥方法对绿藻Caulerpa lentillifera中抗氧化剂、抗糖尿病和抗肥胖活性的影响:体外研究
IF 4.6 2区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-10-22 DOI: 10.1016/j.algal.2024.103768
Marine algae, particularly Caulerpa lentillifera (C. lentillifera), have gained attention as potent sources of bioactive compounds with diverse health benefits. Despite its promising bioactivity, the influence of drying methods on its health-promoting properties remains underexplored. This study investigates the impact of different drying methods on the antioxidant, antidiabetic, and antiobesity activities of C. lentillifera. Fresh samples (GACL-FS) and those dried using air-fryer (GACL-AFD) and microwave (GACL-MD) methods were evaluated for carrageenan content, protein composition, and bioactive potentials. GACL-MD preserved the highest levels of protein, while fresh samples retained the highest carrageenan content. Antioxidant assays revealed superior performance in GACL-MD samples, showing significant DPPH and ABTS inhibition compared to Trolox standards. Similarly, antidiabetic assays demonstrated comparable α-glucosidase inhibition across all drying methods, suggesting robust retention of antidiabetic properties. Antiobesity activity, evaluated through lipase inhibition assays, highlighted GACL-MD's efficacy at lower concentrations, akin to Orlistat, while fresh and air-fryer dried samples showed varied results. In conclusion, C. lentillifera exhibits substantial potential as a functional food ingredient, with the drying method significantly influencing its bioactive profile. Microwave drying emerged as optimal for preserving antioxidant and antiobesity activities, emphasizing the need for tailored drying strategies to enhance nutritional benefits. Future research should focus on optimizing processing techniques and elucidating underlying mechanisms to fully harness C. lentillifera's health-promoting properties in functional food development and public health interventions.
海洋藻类,尤其是蓝藻(Caulerpa lentillifera),作为具有多种健康益处的生物活性化合物的有效来源,已经引起了人们的关注。尽管其生物活性前景广阔,但干燥方法对其健康促进特性的影响仍未得到充分探索。本研究探讨了不同干燥方法对扁豆的抗氧化、抗糖尿病和抗肥胖活性的影响。研究评估了新鲜样品(GACL-FS)以及使用空气油炸法(GACL-AFD)和微波法(GACL-MD)干燥的样品的卡拉胶含量、蛋白质组成和生物活性潜力。GACL-MD 保存了最高水平的蛋白质,而新鲜样品保留了最高的卡拉胶含量。抗氧化试验表明,GACL-MD 样品的抗氧化性能更优越,与 Trolox 标准品相比,它对 DPPH 和 ABTS 的抑制作用明显。同样,抗糖尿病测定显示,所有干燥方法对α-葡萄糖苷酶的抑制作用相当,这表明其具有很强的抗糖尿病特性。通过脂肪酶抑制实验评估的抗肥胖活性突出了 GACL-MD 在较低浓度下的功效,类似于奥利司他,而新鲜样品和风干样品则显示出不同的结果。总之,C. lentillifera 具有作为功能性食品配料的巨大潜力,干燥方法对其生物活性特征有显著影响。微波干燥是保存抗氧化和抗肥胖活性的最佳方法,这强调了需要定制干燥策略来提高营养价值。未来的研究应侧重于优化加工技术和阐明潜在机制,以便在功能食品开发和公共卫生干预中充分利用扁豆的健康促进特性。
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引用次数: 0
The transformation of typical heavy metals during the process for magnetic harvesting and subsequent liquefaction in ethanol of microalgae 微藻磁力收割和随后乙醇液化过程中典型重金属的转化
IF 4.6 2区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-10-22 DOI: 10.1016/j.algal.2024.103766
This study reports the transformation of three typical heavy metals (As(III), Hg(II), and Pb(II)) during the process for magnetic harvesting and subsequent liquefaction in ethanol of microalgae. The magnetic harvesting process of Chlorella Vulgaris (CV) was simulated using a co-precipitation method, achieving the highest magnetic harvesting ratio of 98.95 % at a pH of 8 and an iron-algae ratio of 0.4 g/g. Then the magnetically harvested CV was subjected to liquefaction in ethanol to explore the speciation and migration of As(III), Hg(II), and Pb(II). The environmental risk assessment of above three heavy metals in the bio-oil and biochar was assessed according to the Risk Assessment Code (RAC). Hg(II) and Pb(II) were found to be effectively stabilized and immobilized in the biochar, while As(III) exhibited a propensity to migrate into the bio-oil and existed mostly in the dangerous speciation such as fraction associated with Fe and Mn oxides and fraction bound to organic matter. It reflects a high environmental risk and necessitates a pre-removal treatment.
本研究报告了三种典型重金属(As(III)、Hg(II)和Pb(II))在微藻磁性收获和乙醇液化过程中的转化。采用共沉淀法模拟了小球藻(CV)的磁性收获过程,在 pH 值为 8 和铁藻比为 0.4 g/g 的条件下,最高磁性收获率达到 98.95%。然后,将磁性收获的 CV 在乙醇中进行液化,以探究 As(III)、Hg(II) 和 Pb(II) 的种类和迁移情况。根据风险评估准则(RAC)对生物油和生物炭中的上述三种重金属进行了环境风险评估。结果发现,汞(II)和铅(II)在生物炭中得到了有效的稳定和固定,而砷(III)则表现出迁移到生物油中的倾向,并主要以危险的形态存在,如与铁和锰氧化物相关的部分以及与有机物结合的部分。这反映了较高的环境风险,需要进行预去除处理。
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引用次数: 0
Isolation and identification of thermophilic cyanobacterium inhabiting Shiv Kund (Sohna hot spring), India through polyphasic approach: Bioprospecting in varying nitrogen environment for biotechnological interest particularly for biofuel potential 通过多相法分离和鉴定栖息在印度 Shiv Kund(Sohna 温泉)的嗜热蓝藻:在不同氮环境中进行生物勘探,特别是生物燃料潜力方面的生物技术研究
IF 4.6 2区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-10-21 DOI: 10.1016/j.algal.2024.103757
Identifying a potential candidate having optimal growth characteristics and high-value biotechnological attributes, especially biofuel profile in the era of high rising renewable energy demands is necessary for a sustainable algal-biorefinery perspective. Thermophilic cyanobacteria are scarcely investigated for their industrial potential. In this regard, a preliminary study was performed to isolate a thermophilic cyanobacterium from Shiv Kund, Sohna hot spring, Gurugram, India. The polyphasic approach with molecular identification through 16SrRNA and phylogenetic analysis identified the isolated thermophilic cyanobacterium as Fischerella thermalis PCC 7521 based on the 99.15 % similarity in the NCBI-BLASTn results. Further, the phenotypic visualization under light microscopy and Scanning Electron Microscope (SEM) confirms the true-branching filamentous nitrogen fixing cyanobacterium. To examine its potential for a biotechnological perspective, the effect of different nitrogen concentrations in the growth medium on the biochemical profiling was studied. The highest biomass production and biomass productivity per day obtained was 1041.6 mg/L and 42.33 ± 1.52 mg/L/d⁎⁎(p < 0.01) in 0.1875 g/L (N/8) and 1.5 g/L (N) sodium nitrate concentration respectively. The effect of the growth phase on the biochemical profiling was also prominent and studying optimization helps in the cost-effective production of the required product. Surprisingly, nitrogen starvation has resulted in the increased production of pigments (chlorophyll, phycocyanin and carotenoids), proteins and lipids. The maximum carbohydrate production observed was under complete nitrogen availability (N) in the growth medium with 37.4 % more production in N than complete nitrogen starvation (N0) on 15th day of the growth cycle. Interestingly, the total lipid production observed was 457.41 ± 18.3 μg/mg (p ≤ 0.05) in N0 condition and 519.28 ± 21.3 μg/mg ⁎⁎(p < 0.01) in N/8 condition on 15th and 20th day of the growth cycle respectively. The effect of nitrogen concentrations on the C/N ratio and different functional groups were also examined. Overall, the results indicated F. thermalis PCC 7521 is a promising candidate for different biotechnological fields, especially biofuel production.
在可再生能源需求不断攀升的时代,要实现藻类生物炼制的可持续发展,就必须找到具有最佳生长特性和高价值生物技术属性(尤其是生物燃料特性)的潜在候选藻类。对于嗜热蓝藻的工业潜力,目前还鲜有研究。为此,我们进行了一项初步研究,从印度古鲁格拉姆的 Shiv Kund、Sohna 温泉中分离出一种嗜热蓝藻。根据 NCBI-BLASTn 结果中 99.15 % 的相似度,通过 16SrRNA 分子鉴定和系统进化分析的多相方法确定分离出的嗜热蓝藻为 Fischerella thermalis PCC 7521。此外,在光学显微镜和扫描电子显微镜(SEM)下观察到的表型也证实了这是一种真分支丝状固氮蓝藻。为了从生物技术角度考察其潜力,研究了生长培养基中不同氮浓度对生化分析的影响。在硝酸钠浓度为 0.1875 g/L (N/8) 和 1.5 g/L (N) 时,生物量产量和生物量生产率最高,分别为 1041.6 mg/L 和 42.33 ± 1.52 mg/L/d⁎⁎(p < 0.01)。生长阶段对生化分析的影响也很显著,研究优化有助于以具有成本效益的方式生产所需的产品。令人惊讶的是,氮饥饿导致色素(叶绿素、藻蓝蛋白和类胡萝卜素)、蛋白质和脂质的产量增加。在生长周期的第 15 天,观察到碳水化合物产量最高的是在生长培养基完全供氮(N)的情况下,N 比完全缺氮(N0)的产量高出 37.4%。有趣的是,在生长周期的第 15 天和第 20 天,在 N0 条件下观察到的总脂产量分别为 457.41 ± 18.3 μg/mg ⁎(p ≤ 0.05)和 519.28 ± 21.3 μg/mg ⁎⁎(p < 0.01)。此外,还研究了氮浓度对碳/氮比和不同官能团的影响。总之,研究结果表明 F. thermalis PCC 7521 有希望用于不同的生物技术领域,尤其是生物燃料生产。
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引用次数: 0
Impact of different food-grade protein extraction methods on the proteomic profile and potential allergenicity of Spirulina (Arthrospira platensis) 不同食品级蛋白质提取方法对螺旋藻蛋白质组谱和潜在过敏性的影响
IF 4.6 2区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-10-21 DOI: 10.1016/j.algal.2024.103765
Various optimized methods for cell disintegration and protein extraction of algae have been reported, but there is a lack of information regarding the proteomic characterization of the extracted proteins; thus, systematic verification of the protein profiles to find potentially exploitable proteins, while minimizing allergenicity risks, is profitable. The method described in this study was developed to thoroughly investigate the proteome characterization of A. platensis following aqueous protein extraction by high-shear homogenization and pH shift. Liquid chromatography-tandem mass spectrometry was applied to assess the proteome obtained from four protein extraction methods using data-dependent proteome analysis. Overall, 699 proteins were identified; however, each extraction method identified unique proteins. The maximum number of exclusive proteins was observed using a combination of high-shear homogenization and acidic pH. The evaluation of protein abundance revealed a clear distinction between proteins isolated from pH 2 treatment compared to those obtained via neutral or basic pH treatment. The applied extraction methods affected the in silico computed physicochemical properties of the proteins. Using the AllerCatPro database, 12 putative allergenic proteins were detected, the most predominant of which were related to the C-phycocyanin beta subunit (P72508). This study demonstrated that extraction methods affect the detection, physicochemical properties, and potential allergenicity of the obtained proteins. Hence, the extraction methods used to obtain novel proteins must consider these aspects before using them as functional foods.
目前已报道了多种用于藻类细胞破碎和蛋白质提取的优化方法,但缺乏有关提取蛋白质的蛋白质组特征的信息;因此,对蛋白质概况进行系统的验证,以找到潜在的可利用蛋白质,同时最大限度地降低过敏风险,是有利可图的。本研究采用高剪切匀浆法和 pH 值偏移法进行水提取蛋白后,开发了一种方法来彻底研究 A. platensis 的蛋白质组特征。采用液相色谱-串联质谱法,利用数据依赖性蛋白质组分析评估了四种蛋白质提取方法获得的蛋白质组。共鉴定出 699 个蛋白质;然而,每种提取方法都鉴定出了独特的蛋白质。使用高剪切匀浆和酸性 pH 组合提取的蛋白质数量最多。对蛋白质丰度的评估显示,与通过中性或碱性 pH 处理分离出来的蛋白质相比,pH 值为 2 的蛋白质有明显的区别。所采用的提取方法影响了蛋白质的硅学计算理化性质。利用 AllerCatPro 数据库,检测到了 12 种推测的致敏蛋白质,其中最主要的与 C-花青素 beta 亚基(P72508)有关。这项研究表明,提取方法会影响所获蛋白质的检测、理化性质和潜在过敏性。因此,在将新型蛋白质用作功能食品之前,提取方法必须考虑到这些方面。
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引用次数: 0
Enhancement of ketocarotenoid production via heterologous expression of orange protein from Ipomoea batatas in indigenous microalga Ettlia sp. 通过在本地微藻 Ettlia sp. 中异源表达来自 Ipomoea batatas 的橙色蛋白提高酮类胡萝卜素的产量
IF 4.6 2区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-10-21 DOI: 10.1016/j.algal.2024.103767
Astaxanthin, one of the most powerful natural antioxidants, is used in high-value industries such as those of cosmetics, nutraceuticals, and food products derived from microalgae. In this study, Ettlia sp. mutants were generated by expressing two types of heterologous orange proteins, IbOr and IbOr-R96H, in which the 96th arginine of IbOr was substituted with histidine derived from Ipomoea batatas to enhance astaxanthin production. The Ett-IbOr-R96H mutant showed a 2.4-fold increase in β-carotene content compared to the wildtype (4.59 mg g−1DCW), reaching 10.82 mg g−1 under high-light conditions via two-phase cultivation. Under the stress treatment combination of high-light intensity and nitrogen deprivation, total carotenoid content increased to 17.24 mg L−1 and 21.94 mg L−1 in Ett-IbOr and Ett-IbOr-R96H, respectively. The astaxanthin and canthaxanthin contents in Ett-IbOr-R96H was 4.89 mg L−1 and 0.47 mg L−1, respectively, which were 1.8- and 1.5-fold higher, respectively, than those in Ett-IbOr. Additionally, photosynthetic efficiency (Fv/Fm) recovered in Ett-IbOr-R96H under dual-stress conditions compared to the wildtype while reactive oxygen species levels decreased throughout the cultivation period. Our findings suggest that the heterologous IbOr expression in Ettlia sp. may be an effective approach for enhancing the production of ketocarotenoids and improving stress resistance for industrial applications.
虾青素是最强大的天然抗氧化剂之一,被用于化妆品、营养保健品和微藻食品等高价值产业。本研究通过表达两种异源橙蛋白(IbOr 和 IbOr-R96H)生成了 Ettlia sp.突变体,其中 IbOr 的第 96 个精氨酸被来自 Ipomoea batatas 的组氨酸取代,以提高虾青素的产量。与野生型(4.59 mg g-1DCW)相比,Ett-IbOr-R96H突变体的β-胡萝卜素含量增加了2.4倍,在高光条件下通过两相培养达到10.82 mg g-1。在高光照强度和氮素剥夺的胁迫处理组合下,Ett-IbOr和Ett-IbOr-R96H的类胡萝卜素总含量分别增加到17.24毫克/升和21.94毫克/升。Ett-IbOr-R96H 的虾青素和角黄素含量分别为 4.89 mg L-1 和 0.47 mg L-1,分别是 Ett-IbOr 的 1.8 倍和 1.5 倍。此外,与野生型相比,Ett-IbOr-R96H 在双重胁迫条件下的光合效率(Fv/Fm)有所恢复,而活性氧水平在整个培养期间均有所下降。我们的研究结果表明,在 Ettlia sp.中异源表达 IbOr 可能是提高酮类胡萝卜素产量和抗逆性的有效方法,可用于工业应用。
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引用次数: 0
Regulatory role of microcystin in the response of microcystin-producing cyanobacteria to elevated CO2: Insights from metabolic profiling 微囊藻毒素在产微囊藻蓝藻对高浓度 CO2 的反应中的调控作用:代谢剖析的启示
IF 4.6 2区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-10-20 DOI: 10.1016/j.algal.2024.103760
The regulatory role of microcystin in the response of microcystin-producing cyanobacteria to elevated CO2 remains poorly understood. To address this gap, this study compared the responses of wild-type toxic Microcystis PCC 7806 and its mcyB-knockout mutant to elevated CO2 using metabolomic profiling under nitrogen (N)-rich and N-poor conditions. Under N-poor conditions, elevated CO2 promoted carbohydrate synthesis and tricarboxylic acid cycle in both strains, without affecting their growth. Under N-rich conditions, both strains exhibited increased biomass with rising CO2 levels, attributed to enhanced carbohydrate synthesis, tricarboxylic acid cycle, glutamate-glutamine cycle, purine synthesis, and arginine synthesis. However, compared to the mutant, the proliferation of wild-type toxic Microcystis was less stimulated by elevated CO2. The difference was associated with its reduced activity in the pentose phosphate pathway, likely linked to microcystin synthesis. Besides, the difference was related to higher utilization of glutamate, arginine, and aspartate due to increased microcystin production, indicating the regulatory role of microcystin in the response of microcystin-producing cyanobacteria to elevated CO2. Importantly, elevated CO2 could enhance microcystin synthesis by promoting the production of carbon backbones (malonyl CoA), amino acids (including arginine, glutamate and aspartate) and methyl donors (S-adenosylmethionine) of the wild-type toxic Microcystis PCC 7806. Notably, sufficient nitrogen sources were required for increased amino acid and methyl donors synthesis at high CO2 concentration. The discovery revealed underlying mechanisms behind the potential for elevated CO2 levels to increase toxicity risk associated with Microcystis blooms.
人们对微囊藻毒素在产微囊藻蓝藻对高浓度 CO2 的反应中的调控作用仍然知之甚少。为了填补这一空白,本研究比较了野生型毒性微囊藻 PCC 7806 及其 mcyB 基因敲除突变体在富氮和贫氮条件下对高浓度 CO2 的响应。在贫氮条件下,高浓度 CO2 促进了两株菌株的碳水化合物合成和三羧酸循环,但不影响其生长。在富氮条件下,随着二氧化碳水平的升高,两株菌株的生物量都有所增加,这归因于碳水化合物合成、三羧酸循环、谷氨酸-谷氨酰胺循环、嘌呤合成和精氨酸合成的增强。然而,与突变体相比,野生型毒性微囊藻的增殖受二氧化碳升高的刺激较小。这种差异与其磷酸戊糖途径活性降低有关,而磷酸戊糖途径可能与微囊藻毒素的合成有关。此外,这种差异还与微囊藻毒素产量增加导致谷氨酸、精氨酸和天冬氨酸的利用率提高有关,表明微囊藻毒素在产微囊藻蓝藻对高浓度 CO2 的反应中起着调节作用。重要的是,高浓度 CO2 可促进野生型毒性微囊藻 PCC 7806 的碳骨架(丙二酰 CoA)、氨基酸(包括精氨酸、谷氨酸和天门冬氨酸)和甲基供体(S-腺苷蛋氨酸)的产生,从而增强微囊藻毒素的合成。值得注意的是,在高浓度二氧化碳条件下,氨基酸和甲基供体的合成需要充足的氮源。这一发现揭示了二氧化碳浓度升高可能增加与微囊藻藻华相关的毒性风险的潜在机制。
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Algal Research-Biomass Biofuels and Bioproducts
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