Algal Research-Biomass Biofuels and Bioproducts最新文献_第2页

Development of chitosan-based active composite films incorporating Chlorella vulgaris oil for active food packaging 含小球藻油壳聚糖基活性食品包装复合膜的研制

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2026-01-20 DOI: 10.1016/j.algal.2026.104542

Xiaotong Zou , Shaohua Zhao , Ke Liu , Lingtao Li , Miaosen Zhang , Rui Lu , Changqing Fang

The increasing environmental burden associated with petroleum-based plastic packaging has stimulated growing interest in microalgae-derived bio-based materials for sustainable food packaging applications. In this study, active composite films based on chitosan (CS) and Chlorella vulgaris (CV) oil were prepared using a solution-casting method. Scanning electron microscopy (SEM) observations indicated that the incorporation of CV oil significantly altered the film morphology, resulting in uniformly distributed porous microstructures. Mechanical property analysis showed a gradual decrease in tensile strength, accompanied by an initial increase followed by a decline in elongation at break, with a maximum enhancement of 66%. The surface hydrophobicity of the films increased with increasing CV oil content, as evidenced by an increase in water contact angle up to 83.99°, while water solubility, swelling ratio, and water vapor permeability were correspondingly reduced. Optical measurements revealed a pronounced decrease in UV–visible light transmittance, indicating improved light-barrier performance. Furthermore, bioactive compounds present in CV oil enhanced the antioxidant and antimicrobial properties of the films, with the DPPH radical scavenging activity increasing from 43.17% to 73.45% and effective inhibition observed against Escherichia coli and Staphylococcus aureus. These results demonstrate the potential of CV oil as a functional microalgae-derived additive for the development of sustainable and bioactive packaging films.

与石油基塑料包装相关的日益增加的环境负担刺激了对微藻衍生的生物基材料用于可持续食品包装应用的日益增长的兴趣。本研究采用溶液浇铸法制备了壳聚糖（CS）和小球藻（CV）油基活性复合膜。扫描电镜（SEM）观察表明，CV油的掺入显著改变了膜的形貌，形成了均匀分布的多孔微观结构。力学性能分析表明，拉伸强度逐渐降低，断裂伸长率先升高后下降，最大提高66%。随着CV油含量的增加，膜的表面疏水性增加，水接触角增加至83.99°，而水溶性、溶胀率和水蒸气渗透率相应降低。光学测量显示紫外-可见光透过率显著降低，表明光屏障性能得到改善。此外，CV油中含有的生物活性物质增强了膜的抗氧化和抗菌性能，DPPH自由基清除能力从43.17%提高到73.45%，对大肠杆菌和金黄色葡萄球菌有明显的抑制作用。这些结果表明，CV油作为一种功能性微藻衍生添加剂，具有开发可持续和生物活性包装薄膜的潜力。

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引用次数: 0

Removal of polypropylene microplastics from water by microalgae Desmodesmus sp.: Influences, kinetics and mechanisms 微藻对水中聚丙烯微塑料的去除：影响、动力学和机制

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2026-01-20 DOI: 10.1016/j.algal.2026.104539

Qing Xie , Xiaoai Lin , Lang Tan , Longzao Luo , Shuang Luo , Xiaosong Tian , Qing Cheng , Shao Zhang , Yujing Wang

Microplastics (MPs) pollution in Poyang Lake, China, necessitates the implementation of sustainable remediation strategies. This research developed a Microalgae-based Microplastic Removal System (MMRS) aimed at the removal of polypropylene microplastics (PP-MPs). The findings indicated that the efficacy of PP-MP removal by the MMRS was influenced by several factors, including the particle size and concentration of the microplastics, the pH level, and the initial concentration of microalgal inoculation. Specifically, under conditions where the microalgal concentration was maintained at 0.4 g/L, the microplastic concentration at 16 mg/L, the particle size at 6.5 μm, and the pH at 7.0, the MMRS achieved a removal efficiency of 72% for PP-MPs. The removal process conformed to a pseudo-first-order kinetic model. The primary mechanism facilitating the removal of PP-MPs by the MMRS was identified as adsorption, which was driven by the secretion of extracellular polymeric substances. Overall, the MMRS presents a promising environmentally sustainable approach for the bioremediation of microplastics in freshwater systems, though scalability requires further investigation.

鄱阳湖的微塑料污染需要实施可持续的修复策略。本研究开发了一种基于微藻的微塑料去除系统，旨在去除聚丙烯微塑料（PP-MPs）。结果表明，MMRS去除PP-MP的效果受微塑料粒径和浓度、pH值、微藻接种初始浓度等因素的影响。在微藻浓度为0.4 g/L、微塑料浓度为16 mg/L、粒径为6.5 μm、pH为7.0的条件下，MMRS对PP-MPs的去除率可达72%。去除过程符合准一级动力学模型。MMRS去除PP-MPs的主要机制是吸附，这是由细胞外聚合物质的分泌驱动的。总的来说，MMRS为淡水系统中的微塑料生物修复提供了一种有前景的环境可持续方法，尽管可扩展性需要进一步研究。

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引用次数: 0

Research trends and hotspots of microalgae protein as a functional ingredient in plant-based foods: A bibliometric analysis based on the web of science 微藻蛋白作为植物性食品功能成分的研究趋势与热点：基于科学网的文献计量学分析

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2026-01-20 DOI: 10.1016/j.algal.2026.104541

Jiayi He , Haoxie Xu , Keyu Lu , Chunli Guo , Ming Du , Ruochen Jia

This bibliometric study maps the research landscape of microalgae protein as a functional ingredient in plant-based foods, utilizing data from the Web of Science (WOS) Core Collection from 2015 to 2024. The study analyzes trends in publication volume, collaboration networks, keyword evolution, and citation bursts, revealing the rapid growth and increasing significance of microalgae proteins in the field of sustainable food systems. The research identifies key themes, such as protein functionality, environmental sustainability, and the use of microalgae in food formulations like meat analogs and dairy alternatives. Insights into the collaboration networks across countries, institutions, and authors highlight the global nature of the research, with emerging contributions from developing countries. Temporal keyword analysis underscores the shift from foundational biological studies to applied food science, with a rising focus on antioxidant properties and sustainability. This study provides valuable insights into the current state of microalgae protein research and identifies future research directions aimed at optimizing production, enhancing functional properties, and integrating microalgae proteins into the global food system.

本文献计量学研究利用Web of Science (WOS) Core Collection从2015年到2024年的数据，绘制了微藻蛋白作为植物性食品功能成分的研究图景。本研究分析了微藻蛋白在发文量、合作网络、关键词演变和引文爆发等方面的趋势，揭示了微藻蛋白在可持续粮食系统领域的快速增长和日益重要的意义。该研究确定了关键主题，如蛋白质功能、环境可持续性以及微藻在肉类类似物和乳制品替代品等食品配方中的使用。对跨国家、机构和作者的合作网络的洞察突出了该研究的全球性质，以及发展中国家的新贡献。时间关键词分析强调了从基础生物学研究到应用食品科学的转变，越来越关注抗氧化特性和可持续性。本研究为微藻蛋白的研究现状提供了有价值的见解，并确定了未来的研究方向，旨在优化生产，增强功能特性，并将微藻蛋白融入全球食品体系。

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引用次数: 0

Mechanisms for enhanced antibiotic removal and lipid production by a cyanobacteria-bacteria consortium in an optimized baffled photobioreactor 在优化的折流板光生物反应器中，蓝藻-细菌联合体增强抗生素去除和脂质产生的机制

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2026-01-20 DOI: 10.1016/j.algal.2026.104536

Ziqi Yu , Guannan Lin , Yawen Qi , Ying Liu , Jian Zhang

This study optimized seven operating parameters for purifying antibiotic-polluted wastewater through orthogonal experiments including TN (15 mg/L), TP (2 mg/L), COD (50 mg/L), antibiotic concentration (5 μg/L), initial cyanobacterial biomass (200 mg/L), light intensity (4000 lx), and hydraulic retention time (96 h). Using these conditions, a continuous wastewater treatment system was established in a baffled photobioreactor containing a symbiotic consortium of immobilized Synechococcus and indigenous wastewater bacteria. During a 36-d stable operation, the system achieved high lipid productivity of 30.67–68.17 mg/(L·d) and remarkable removal rates of up to 90.77%, 87.88%, 87.50%, and 79.59% for TN, TP, COD, and a typical antibiotic (sulfamethoxazole), respectively. Metatranscriptomic analysis revealed the molecular mechanisms in the cyanobacteria-bacteria consortium, namely: (i) upregulation of genes (GlnA, GlnK, AmtB), GO biological processes, and KEGG pathways related to nitrogen metabolism facilitated nitrogen assimilation from wastewater; (ii) overexpression of genes related to ATP synthesis (AtpE) and phosphate transport (PstS) enhanced phosphorus uptake from wastewater; (iii) upregulation of a stress response gene (sodA), GO biological processes related to antibiotic degradation, and KEGG pathways associated with the degradation of various organic pollutants enabled efficient sulfamethoxazole and COD removal; (iv) correlation between photosynthesis-related genes and bacterial genes, as well as the upregulated signaling-related GO biological processes, confirmed cyanobacteria-bacteria cooperation; and (v) upregulation of functional genes and KEGG pathways representing photosynthesis, carbon fixation, and fatty acid synthesis directed carbon flux toward lipid production. Overall, the system demonstrates potential for simultaneous wastewater remediation and biofuel feedstock production.

本研究通过正交试验，优化了TN （15 mg/L）、TP （2 mg/L）、COD （50 mg/L）、抗生素浓度（5 μg/L）、初始蓝藻生物量（200 mg/L）、光照强度（4000 lx）、水力停留时间（96 h） 7个操作参数对抗生素污染废水的净化效果。在此条件下，在一个包含固定化聚球菌和本地废水细菌共生联合体的挡板光生物反应器中建立了连续废水处理系统。在36 d的稳定运行中，该系统的脂质产率达到30.67 ~ 68.17 mg/(L·d)，对TN、TP、COD和一种典型抗生素磺胺甲新唑的去除率分别达到90.77%、87.88%、87.50%和79.59%。超转录组学分析揭示了蓝藻-细菌联盟的分子机制，即：(1)与氮代谢相关的基因（GlnA、GlnK、AmtB）、GO生物过程和KEGG途径的上调促进了废水中氮的同化；（ii）与ATP合成（AtpE）和磷酸盐运输（PstS）相关的基因过表达增强了废水中磷的吸收；（iii）应激反应基因（sodA）的上调，与抗生素降解相关的氧化石墨烯生物过程，以及与各种有机污染物降解相关的KEGG途径，使磺胺甲恶唑和COD的有效去除成为可能；（iv）光合作用相关基因与细菌基因的相关性，以及与氧化石墨烯信号相关的生物过程的上调，证实了蓝藻-细菌的合作；(v)代表光合作用、碳固定和脂肪酸合成的功能基因和KEGG通路的上调将碳通量导向脂质生产。总的来说，该系统显示了同时进行废水修复和生物燃料原料生产的潜力。

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引用次数: 0

Physiological, biochemical, and cytotoxic characterization of two UV-C mutant strains of the microalga Porphyridium purpureum (Rhodophyta): An indoor and outdoor study 两种紫红藻UV-C突变株的生理、生化和细胞毒特性：室内和室外研究

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2026-01-19 DOI: 10.1016/j.algal.2026.104533

Pablo Castro-Varela , Roberto Teófilo Abdala-Díaz , Félix L. Figueroa , Patricia I. Gómez

Porphyridium purpureum is a red microalga that contains phycoerythrin (R-PE), exopolysaccharides (EPS) and polyunsaturated fatty acids (PUFAs): linoleic acid (LA), eicosapentaenoic acid (EPA), and arachidonic acid (AA). The optimization of culture conditions remains a major challenge for enhancing biomass production on a large scale. In this study, a random mutagenesis-selection approach to enhance the physiological and biochemical traits of P. purpureum was used. UV-C radiation (254 nm) served as the mutagenic agent, while dim light and the fatty acid biosynthesis inhibitor Cerulenin, were employed as selection agents. Four mutant strains (A-4, B-3, B-7, and B-11) were selected and evaluated at the laboratory scale (1 L). According to their growth and biochemical attributes, strains B-7 and B-11 were selected and used for outdoor trials in 350 L tubular photobioreactor. The strains B-7 and B-3 in 350-L trials achieved similar percentages of total polyunsaturated fatty acids (ranging from 60 to 64%) compared to the wild-type strain. However, only the B-7 strain showed an increase in total lipid accumulation, rising from 5.9% to 6.5% relative to the wild type; additionally, LA and ARA increased by 49.5% and 52.3.7%, respectively, while EPA decreased by 56.7% compared to the wild type. Concerning phycoerythrin, improvements were observed only in strain B-7 (from 14.4 to 20.6 mg g⁻¹ dry biomass). The biomass production per culture volume increased by approximately 53.2% for B-7 and 35% for B-3, respectively, relative to the wild type, indicating that notable properties of the mutant strains could be observed at a large culture scale. Regarding the potential use of these strains in nutraceutical applications, cytotoxicity tests of strains B-3 and B-7 showed no harmful effects on healthy human cell lines. Furthermore, both strains demonstrated some antitumoral activity against colon cancer HCT-116 cells. This study demonstrated that random mutagenesis-selection is a valuable approach for selecting new P. purpureun strains for biotechnological applications.

紫斑卟啉（Porphyridium purpureum）是一种红色微藻，含有植红蛋白（R-PE）、外多糖（EPS）和多不饱和脂肪酸（PUFAs）：亚油酸（LA）、二十碳五烯酸（EPA）和花生四烯酸（AA）。优化培养条件仍然是大规模提高生物质生产的主要挑战。本研究采用随机诱变-选择方法提高紫花假单胞菌的生理生化特性。紫外- c辐射（254 nm）作为诱变剂，弱光和脂肪酸生物合成抑制剂Cerulenin作为选择剂。选择4个突变株（A-4、B-3、B-7和B-11）在实验室规模（1 L）进行评估。根据菌株B-7和B-11的生长特性和生化特性，选择菌株B-7和B-11在350 L管状光生物反应器中进行室外试验。菌株B-7和B-3在350-L试验中获得的总多不饱和脂肪酸百分比与野生型菌株相似（60 - 64%）。但只有B-7菌株的总脂质积累较野生型增加，从5.9%增加到6.5%；与野生型相比，LA和ARA分别增加了49.5%和52.3.7%，EPA减少了56.7%。关于藻红蛋白，只有菌株b7的干生物量从14.4 mg g−1提高到20.6 mg g−1。与野生型相比，B-7和B-3的单位培养体积生物量分别增加了约53.2%和35%，表明在大规模培养中可以观察到突变菌株的显著特性。关于这些菌株在营养保健应用中的潜在用途，菌株B-3和B-7的细胞毒性试验显示对健康的人类细胞系没有有害影响。此外，两株菌株对结肠癌HCT-116细胞均表现出一定的抗肿瘤活性。该研究表明，随机诱变选择是一种有价值的选择新的紫癜性假单胞菌的生物技术应用的方法。

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引用次数: 0

Research on the application of machine learning in optimizing the heavy metal adsorption of algae-based adsorbents 机器学习在优化藻类吸附剂对重金属吸附中的应用研究

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2026-01-19 DOI: 10.1016/j.algal.2026.104528

Aoqiang Li , Yang Peng , Shen Yao , Lei Zhang , Fanwei Meng

Microalgae are promising biological adsorbents for heavy metal removal from aqueous solutions, and machine learning (ML) is a powerful tool for optimizing their adsorption performance. This study developed an ML framework to optimize algae-based adsorbents heavy metal removal efficacy and clarify the underlying regulatory mechanisms. A dataset containing 3692 data points (10 input variables related to microalgal biological traits and adsorption conditions) was constructed by systematically reviewing 71 published literatures, with adsorption efficiency as the output variable. The dataset was further partitioned into 12 sub-datasets in accordance with the principle that one type of microalga is designated for adsorbing one specific heavy metal. Ten ML models were trained and validated, and Shapley Additive Explanations (SHAP) analysis combined with Partial Dependence Plots (PDP) was used to interpret feature importance and variable interactions. Results showed that the Extra Trees model outperformed other models, achieving a maximum test-set coefficient of determination (R²) of 0.9653 and a minimum root mean square error (RMSE) of 4.7024. Feature importance ranking revealed that experimental conditions exerted a more significant influence on adsorption efficiency than microalgal biological characteristics. Additionally, SHAP and PDP analyses identified key nonlinear antagonistic interactions between biomass concentration and initial heavy metal concentration. The sub-dataset prediction mode was proven suitable for pre-experiment scenarios due to its reduced computational cost, while the full-dataset mode was more applicable for broad-spectrum wastewater treatment predictions. This work provides theoretical support for the rational development and optimization of algae-based heavy metal adsorbents.

微藻是一种很有前途的去除水中重金属的生物吸附剂，而机器学习（ML）是优化其吸附性能的有力工具。本研究建立了一个ML框架，以优化藻类吸附剂的重金属去除效果，并阐明其潜在的调节机制。以吸附效率为输出变量，系统回顾71篇已发表的文献，构建了包含3692个数点（10个输入变量与微藻生物学特性和吸附条件相关）的数据集。按照一种微藻吸附一种特定重金属的原则，将数据集进一步划分为12个子数据集。对10个ML模型进行了训练和验证，并使用Shapley加性解释（SHAP）分析结合偏相关图（PDP）来解释特征重要性和变量相互作用。结果表明，Extra Trees模型优于其他模型，最大检验集决定系数（R2）为0.9653，最小均方根误差（RMSE）为4.7024。特征重要性排序显示，实验条件对吸附效率的影响比微藻生物学特性的影响更显著。此外，SHAP和PDP分析确定了生物量浓度与初始重金属浓度之间的关键非线性拮抗相互作用。子数据集预测模式由于计算成本较低，适用于实验前场景，而全数据集预测模式更适用于广谱污水处理预测。本研究为藻基重金属吸附剂的合理开发和优化提供了理论支持。

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引用次数: 0

Insights into interactions of scytonemin and its derivatives with dipeptidyl peptidase-IV 舒张素及其衍生物与二肽基肽酶- iv的相互作用

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2026-01-19 DOI: 10.1016/j.algal.2026.104529

Anshul Kaushik , Ritu Kothari , Kiran Bala

Dipeptidyl peptidase-IV (DPP-IV) is a promising target for treating diabetes, which is a major global healthcare challenge. Existing DPP-IV inhibitor drugs have been associated with side effects, hence there is a pressing need to discover therapeutic alternatives. Algae have emerged as an important source of structurally diverse natural metabolites with pharmaceutical activities. This study aims to combine systematic in-silico screening with detailed molecular interactions analysis, and in-vitro DPP-IV enzyme inhibition assay to identify best algal metabolite as inhibitor. Potential allosteric surface binding sites were predicted in DPP-IV. To gain insight into interactions, conformation stability and flexibility upon ligand binding were analyzed. Virtual screening of 69 metabolites identified 12 ligands scoring better than positive control drug sitagliptin (−8.73 ± 0.37 kcal/mol) including luteolin-7-glucoside, hesperidin, epigallocatethin, phlorofucofuroeckol B), calothrixin. The highest scoring metabolite was identified as scytonemin (−10.86 ± 0.05 kcal/mol). MMPBSA analysis of interactions between derivatives of scytonemin and DPP-IV revealed differences in binding energy score. Compared to scytonemin (−21.18 ± 2.11 kcal/mol), dimethoxyscytonemin showed improved binding affinity (−26.41 ± 1.93 kcal/mol), whereas tetramethoxyscytonemin and scytonin exhibited reduced energy (−16.93 ± 3.06 kcal/mol and − 12.29 ± 4.24 kcal/mol, respectively). Analysis of scytonemin and its derivatives interactions with amino acid residues in DPP-IV binding site identified Trp-695 and Phe-674 as key residues for binding. Additionally, scytonemin exhibited potent DPP-IV enzyme inhibitory activity (IC₅₀ = 2.75 ± 1.70 nM). In conclusion, this study identifies that scytonemin has good activity as DPP-IV inhibitor, supporting its potential as antidiabetic therapeutic.

二肽基肽酶- iv （DPP-IV）是治疗糖尿病的一个有希望的靶点，糖尿病是一个主要的全球卫生保健挑战。现有的DPP-IV抑制剂药物具有副作用，因此迫切需要发现治疗替代药物。藻类已成为具有药用活性的结构多样的天然代谢物的重要来源。本研究旨在结合系统的计算机筛选和详细的分子相互作用分析，以及体外DPP-IV酶抑制实验来确定最佳的藻类代谢物作为抑制剂。预测了DPP-IV的潜在变构表面结合位点。为了深入了解相互作用，分析了配体结合时的构象稳定性和灵活性。对69种代谢物进行虚拟筛选，鉴定出12种配体得分优于阳性对照药物西格列汀（- 8.73±0.37 kcal/mol），包括木叶草素-7-葡萄糖苷、橙皮苷、表没食子苷、苯二酚氟化钙B)、钙思草素。评分最高的代谢物为肌张力蛋白（- 10.86±0.05 kcal/mol）。MMPBSA分析显示，舒张素衍生物与DPP-IV之间的相互作用存在结合能评分差异。与四甲氧基丝胞胺（- 16.93±3.06 kcal/mol）和四甲氧基丝胞胺（- 12.29±4.24 kcal/mol）相比，二甲氧基丝胞胺的结合亲和力（- 21.18±2.11 kcal/mol）有所提高（- 26.41±1.93 kcal/mol），而四甲氧基丝胞胺和丝胞胺的结合能力则有所降低（- 16.93±3.06 kcal/mol）。通过对胞浆胺及其衍生物与DPP-IV结合位点氨基酸残基的相互作用分析，发现Trp-695和ph -674是结合的关键残基。此外，舒张素表现出较强的DPP-IV酶抑制活性（IC50 = 2.75±1.70 nM）。综上所述，本研究确定了舒张素作为DPP-IV抑制剂具有良好的活性，支持其作为抗糖尿病药物的潜力。

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引用次数: 0

The effect and mechanism of Synechococcus sp. PCC 7942 harboring VP26 in the immunity of Litopenaeus vannamei against WSSV 含VP26的聚球菌PCC 7942对凡纳滨对虾免疫WSSV的作用及机制

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2026-01-19 DOI: 10.1016/j.algal.2026.104532

Xiang Ding , Shengyu Liao , Chan Zhu , Xiaofeng Gao , Hao Xing , Rui Jia

White spot syndrome virus (WSSV) has become one of the most widely spread deadly viruses in shrimp farming because of its wide host range, fast spreading speed, and high lethality. Synechococcus sp. PCC 7942 is a safe, edible, noncytotoxic cyanobacterium and is a good expression vector. As a tegument protein of WSSV, VP26 plays an important role in the stage of virus reproduction. This study is mainly about constructing Synechococcus sp. PCC 7942 harboring VP26, as well as detecting the expression of host immune pathway marker genes (Dorsal, Relish, STAT, c-Jun, and ALF1), metabolic enzyme activities including lysozyme (LZM), superoxide dismutase (SOD), phenoloxidase (PO), catalase (CAT), glutamate dehydrogenase (GDH), and fatty acid synthase (FAS), and mortality in vivo to evaluate its prevention and treatment effect on WSSV. The study showed that the oral administration of transformed Synechococcus sp. PCC 7942 followed by the challenge led to a relative survival rate of 80% compared with 0% of the positive group. The immune pathway marker gene expression levels and activity of 5 metabolic enzymes tested in the experiment group and the positive group showed an upward trend and reached a peak at 12 h, after which they decreased within 12–24 h. Nevertheless, the activity of LZM in these groups increased within 0–6 h, peaked at 6 h, then decreased. In all the expression and enzyme activity tests, the results in the experiment group were lower than those in the positive group. These results indicated that Synechococcus sp. PCC 7942 harboring VP26 had a positive effect on protecting shrimp from WSSV.

白斑综合征病毒（WSSV）因其宿主范围广、传播速度快、致死率高，已成为对虾养殖中传播最广泛的致命病毒之一。聚藻球菌（Synechococcus sp. pcc7942）是一种安全、可食用、无细胞毒性的蓝藻，是一种良好的表达载体。VP26作为WSSV的被膜蛋白，在病毒繁殖阶段起着重要的作用。本研究主要通过构建携带VP26基因的聚球菌PCC 7942，检测宿主免疫途径标记基因（Dorsal、穗、STAT、c-Jun、ALF1）的表达，体内溶菌酶（LZM）、超氧化物歧化酶（SOD）、酚氧化酶（PO）、过氧化氢酶（CAT）、谷氨酸脱氢酶（GDH）、脂肪酸合成酶（FAS）等代谢酶活性及致死率，评价其对WSSV的防治效果。研究表明，口服转化聚球菌PCC 7942后攻毒，相对存活率为80%，阳性组为0%。试验组和阳性组免疫通路标记基因表达水平和5种代谢酶活性均呈上升趋势，在12 h达到峰值，12 - 24 h内下降，而各组LZM活性在0-6 h内升高，6 h达到峰值，然后下降。在所有的表达和酶活性测试中，实验组的结果低于阳性组。上述结果表明，含VP26的聚球菌PCC 7942对WSSV有一定的保护作用。

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引用次数: 0

From linear to circular bioeconomy: Case study and life cycle assessment of integrating small-scale freshwater aquaculture with microalgae cultivation 从线性到循环生物经济：小规模淡水养殖与微藻养殖相结合的案例研究与生命周期评估

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2026-01-19 DOI: 10.1016/j.algal.2026.104531

Alexander Meneses-Jácome , Sebastián Muñoz-Chinome , Lorena Durán-Ortiz , Astrid Y. Rincón-Hernández , Yohana Castro-Hernández

This study deals with the use of microalgae cultivation system to treat effluents from a small, artisanal fish farm that breed “red tilapia” in Colombia. These types of “aquafarms” have limited technology and high production costs, particularly regarding aquaculture feed inputs. The proposed integrated “aquaculture-microalgae” process aims to recover nutrients and rationalise freshwater intake, whilst the harvested algae biomass partially offsets commercial feed and provides fish farmers with extra income. In this way “circular bioeconomy” notions are brought to this traditional form of aquaculture. The results of this study comprise a life cycle assessment (LCA) based on data extracted from a fully integrated pilot system for fish farming and microalgae (Spirulina) production, using a 6.6 m³ open-raceway pond bioreactor; it also covers the validation of aquafeed replacement with microalgae. LCA methodology has provided a more comprehensive view of the environmental trade-offs and co-benefits associated with the proposed shift from a traditional, linear model of aquaculture to a circular bioeconomy, but it also reveals areas requiring attention for its full maturation and potential replication. The environmental midpoint indicators used to analyse local water pollution, climate change and resource use, return lower values in circular bioeconomy scenarios with “enhanced circularity”, than in the baseline scenario (traditional fish farming). Although the circular bioeconomy model and reference scenario display similar toxicity indicator results, positive change is possible in this respect. Lastly, it is suggested using the harvested biomass for basic “in situ” applications, without major downstream refinement, to be sustainable in isolated, low-income contexts.

本研究涉及使用微藻培养系统来处理哥伦比亚一个养殖“红罗非鱼”的小型手工养鱼场的废水。这些类型的“水产养殖场”技术有限，生产成本高，特别是在水产养殖饲料投入方面。拟议的综合“水产养殖-微藻”过程旨在回收营养物质并使淡水摄入量合理化，而收获的藻类生物量部分抵消了商业饲料，并为养鱼户提供了额外的收入。通过这种方式，“循环生物经济”概念被带入了这种传统的水产养殖形式。本研究的结果包括生命周期评估（LCA），该评估基于从一个完全集成的养鱼和微藻（螺旋藻）生产中试系统中提取的数据，该系统使用6.6立方米的开放式沟道池塘生物反应器；它还涵盖了用微藻替代水产饲料的验证。LCA方法对从传统的线性水产养殖模式向循环生物经济模式转变所带来的环境权衡和共同利益提供了更全面的看法，但它也揭示了需要注意的领域，以使其完全成熟和潜在的复制。用于分析当地水污染、气候变化和资源利用的环境中点指标在“增强循环性”的循环生物经济情景中返回的值低于基线情景（传统养鱼）。尽管循环生物经济模型和参考情景显示出相似的毒性指标结果，但在这方面有可能发生积极的变化。最后，建议将收获的生物质用于基本的“就地”应用，而不需要进行主要的下游提纯，以便在孤立的低收入环境中可持续发展。

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引用次数: 0

Research hotspots and evolutionary trends in microalgae-derived EPA production: A bibliometric review 微藻产生EPA的研究热点和进化趋势：文献计量学综述

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2026-01-15 DOI: 10.1016/j.algal.2025.104505

Qingting Weng , Xiaoai Lin , Longzao Luo , Lang Tan , Qin Liu , Shiping Lu , Guosheng Li , Zhou Zhang

In response to the growing demand for sustainable sources of omega-3 fatty acids, this study provides a comprehensive evaluation of academic and patent advances in eicosapentaenoic acid (EPA) production from microalgae. A bibliometric analysis was conducted using VOSviewer on publications retrieved from the Web of Science Core Collection and patent documents from Derwent Innovations Index between 2005 and 2024. Keyword co-occurrence and clustering analyses were employed to identify major research themes and temporal trends. The results reveal a steady increase in annual publications, divided into three distinct phases. Patent analysis shows parallel growth in commercial activity with a 2–3-year lag behind academic research peaks. Research spans diverse disciplines, including marine biology, biotechnology, and microbiology. Articles were published in 200 journals, 24 of which each published more than 10 articles each. The United States and China were identified as the leading countries in both publication output and collaborative influence. Author co-occurrence analysis indicated that Martin J. Kainz was the most prolific contributor. High-frequency keywords such as “microalgae”, “growth”, and “EPA” reflect central research themes, with clusters highlighting biological traits and environmental factors. Current research focuses on strain selection, lipid composition, metabolic engineering, and commercial applications. Microalgae-derived EPA represents a promising sustainable alternative to fish oil, with significant potential in nutrition, aquaculture, and the food industry.

为了响应对omega-3脂肪酸可持续来源日益增长的需求，本研究对微藻生产二十碳五烯酸（EPA）的学术和专利进展进行了全面评估。利用VOSviewer对2005 - 2024年间Web of Science核心馆藏的出版物和Derwent Innovations Index的专利文献进行了文献计量分析。关键词共现和聚类分析用于确定主要研究主题和时间趋势。结果显示，年度出版物稳步增长，分为三个不同的阶段。专利分析显示，商业活动的平行增长比学术研究峰值滞后2 - 3年。研究跨越多个学科，包括海洋生物学、生物技术和微生物学。在200种期刊上发表文章，其中24种期刊发表10篇以上。美国和中国被确定为在出版物产出和合作影响力方面领先的国家。作者共现分析表明，Martin J. Kainz是最多产的贡献者。高频关键词如“微藻”、“生长”和“EPA”反映了中心研究主题，集群突出了生物特性和环境因素。目前的研究重点是菌株选择、脂质组成、代谢工程和商业应用。微藻衍生的EPA代表了一种有前途的可持续的鱼油替代品，在营养、水产养殖和食品工业中具有巨大的潜力。

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引用次数: 0