Algal Research-Biomass Biofuels and Bioproducts最新文献_第2页

Thermosynechococcus guangdongensis HY-01 sp. nov., a convenient easily transformable thermophilic chassis for photothermobiology 广东热共生球菌HY-01 sp. nov.，一种方便易转换的光热生物学的嗜热性底盘

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2026-04-01 Epub Date: 2026-02-17 DOI: 10.1016/j.algal.2026.104597

Xiangjian Liu , Chen Li , Ying Jiang , Lianghuan Wu , Dawei You , Maurycy Daroch

This study describes the isolation and characterization of Thermosynechococcus guangdongensis HY-01, a novel thermophilic cyanobacterium developed as a microbial chassis for photothermobiology. The strain was isolated from a top stratum of a microbial mat collected at a geothermal area Tangjiaoqiaotou in Guangdong province of China. Its genome, sequenced using a hybrid approach, is the largest reported in the genus at 2.75 Mbp. The expansion of genome could be attributed to the presence of 180 unique genes, many acquired via horizontal gene transfer. These unique genes are often organised into genomic clusters, four of which are composed exclusively of HY-01-specific genes, including two implicated in exopolysaccharide biosynthesis. This genomic feature may contribute to the strain's ecological niche and physiological robustness. Strain delineation as a new species was performed based on the Botanical Code, encompassing 16S rRNA phylogeny, GTDB, Average Nucleotide and Amino Acid Identities together with morphological and habitat data. The polyphasic approach confirmed its status as a new species within the family. Morphologically and physiologically, HY-01 exhibited typical Thermosynechococcus characteristics. Growth profiling demonstrated robust performance across 37–57 °C, with peak at 52 °C and broad optimum between 47 and 57 °C. Critically, a simplified genetic transformation system based on electroporation was successfully established, achieving fully segregated transgenic lines within a fortnight, as validated by diagnostic PCR and protein immunoblotting of His-tagged photosystem I protein psaJ. We conclude that Thermosynechococcus guangdongensis HY-01 represents a genetically tractable and thermotolerant new species, expanding the portfolio of model organisms for studying thermostable photosynthesis and for developing high-temperature biotechnological applications.

本文报道了广东热共生球菌HY-01的分离和鉴定，这是一种新型的嗜热蓝藻，是光热生物学的微生物基础。该菌株分离自广东汤角桥头地热区采集的微生物垫表层。它的基因组是用杂交方法测序的，是该属中报道的最大的基因组，长度为2.75 Mbp。基因组的扩增可归因于180个独特基因的存在，其中许多是通过水平基因转移获得的。这些独特的基因通常被组织成基因组簇，其中四个完全由hy -01特异性基因组成，包括两个与外多糖生物合成有关的基因。这种基因组特征可能有助于菌株的生态位和生理稳健性。根据植物编码，包括16S rRNA系统发育、GTDB、平均核苷酸和氨基酸特征以及形态和栖息地数据，将菌株划分为新种。多相方法证实了其作为科内新种的地位。在形态和生理上，HY-01表现出典型的热聚球菌特征。生长曲线在37-57°C范围内表现强劲，在52°C达到峰值，在47 - 57°C范围内达到最佳。关键是，成功建立了基于电穿孔的简化遗传转化系统，在两周内实现了完全分离的转基因系，并通过诊断PCR和his标记的光系统I蛋白psaJ的蛋白质免疫印迹验证了这一点。我们认为，广东热聚球菌HY-01是一种遗传易感和耐热的新物种，为研究热稳态光合作用和开发高温生物技术提供了新的模式生物组合。

{"title":"Thermosynechococcus guangdongensis HY-01 sp. nov., a convenient easily transformable thermophilic chassis for photothermobiology","authors":"Xiangjian Liu , Chen Li , Ying Jiang , Lianghuan Wu , Dawei You , Maurycy Daroch","doi":"10.1016/j.algal.2026.104597","DOIUrl":"10.1016/j.algal.2026.104597","url":null,"abstract":"<div><div>This study describes the isolation and characterization of <em>Thermosynechococcus guangdongensis</em> HY-01, a novel thermophilic cyanobacterium developed as a microbial chassis for photothermobiology. The strain was isolated from a top stratum of a microbial mat collected at a geothermal area Tangjiaoqiaotou in Guangdong province of China. Its genome, sequenced using a hybrid approach, is the largest reported in the genus at 2.75 Mbp. The expansion of genome could be attributed to the presence of 180 unique genes, many acquired via horizontal gene transfer. These unique genes are often organised into genomic clusters, four of which are composed exclusively of HY-01-specific genes, including two implicated in exopolysaccharide biosynthesis. This genomic feature may contribute to the strain's ecological niche and physiological robustness. Strain delineation as a new species was performed based on the Botanical Code, encompassing 16S rRNA phylogeny, GTDB, Average Nucleotide and Amino Acid Identities together with morphological and habitat data. The polyphasic approach confirmed its status as a new species within the family. Morphologically and physiologically, HY-01 exhibited typical <em>Thermosynechococcus</em> characteristics. Growth profiling demonstrated robust performance across 37–57 °C, with peak at 52 °C and broad optimum between 47 and 57 °C. Critically, a simplified genetic transformation system based on electroporation was successfully established, achieving fully segregated transgenic lines within a fortnight, as validated by diagnostic PCR and protein immunoblotting of His-tagged photosystem I protein psaJ. We conclude that <em>Thermosynechococcus guangdongensis</em> HY-01 represents a genetically tractable and thermotolerant new species, expanding the portfolio of model organisms for studying thermostable photosynthesis and for developing high-temperature biotechnological applications.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"95 ","pages":"Article 104597"},"PeriodicalIF":4.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147386607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Coelastrella sp. biofilm integrated on agave-derived carbon for enhanced anodic performance in biophotovoltaic energy harvesting 龙舌兰类生物膜在龙舌兰衍生碳上的集成，用于提高生物光伏能量收集的阳极性能

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2026-04-01 Epub Date: 2026-02-16 DOI: 10.1016/j.algal.2026.104594

Patrick U. Okoye , Itzel Montes-Roman , Rosa Angelica Guillen-Garcés , Francisco Vera-Lopez Portillo , Diego Lobato-Peralta , Dulce M. Arias-Lizarraga

The development of sustainable electrode materials that allows sufficient accumulation of redox species is key to advancing biophotovoltaic (BPV) technologies. In this study, activated carbon derived from agave fiber was evaluated as an anodic electrode material for BPV devices. Activated carbon samples were synthesized at 600, 700, and 800 °C using different agave biomass-to-KOH impregnation ratios from 1:1 to 1:3. The resulting carbons were comprehensively characterized to analyze their morphology and composition, and to establish structure–performance relationships. Electrochemical evaluation using Coelastrella sp. biofilms revealed that the electrode produced at 700 °C with a 1:2 ratio delivered the best performance, achieving a specific capacity of 3.50 mAh/g. Both impregnation ratio and activation temperature influenced biofilm accumulation, while a balanced meso–micropore structure favored electron transfer from the bulk to the anode surface, ensuring sufficient interaction with photosynthetic microorganisms of Coelastrella sp. The maximum power density achieved was 1.3 mW/m², with an open-circuit voltage of ∼140 mV at the lowest current density (∼6 mA/m²), and a short-circuit current density of ∼24 mA/m². These findings demonstrate the potential of Coelastrella sp. biofilms to enhance the functionality of electrochemical materials by improving electron transfer kinetics, thereby contributing to the development of clean technologies that harness solar energy.

开发可持续的电极材料，使氧化还原物质充分积累，是推进生物光伏技术的关键。在这项研究中，从龙舌兰纤维中提取的活性炭被评价为BPV装置的阳极电极材料。在600、700和800℃的条件下，以不同的龙舌兰生物质与koh的浸渍比例（1:1 ~ 1:3）合成活性炭样品。对所得碳进行了全面表征，分析了其形貌和组成，并建立了结构性能关系。利用Coelastrella sp.生物膜进行电化学评价表明，在700°C下以1:2的比例生产的电极具有最佳性能，达到3.50 mAh/g的比容量。浸透比和活化温度都会影响生物膜的积累，而平衡的中微孔结构有利于电子从体向阳极表面的转移，从而确保与Coelastrella sp.光合微生物的充分相互作用。获得的最大功率密度为1.3 mW/m2，在最低电流密度（~ 6 mA/m2）下开路电压为~ 140 mV，短路电流密度为~ 24 mA/m2。这些发现证明了Coelastrella sp.生物膜通过改善电子传递动力学来增强电化学材料功能的潜力，从而有助于开发利用太阳能的清洁技术。

{"title":"Coelastrella sp. biofilm integrated on agave-derived carbon for enhanced anodic performance in biophotovoltaic energy harvesting","authors":"Patrick U. Okoye , Itzel Montes-Roman , Rosa Angelica Guillen-Garcés , Francisco Vera-Lopez Portillo , Diego Lobato-Peralta , Dulce M. Arias-Lizarraga","doi":"10.1016/j.algal.2026.104594","DOIUrl":"10.1016/j.algal.2026.104594","url":null,"abstract":"<div><div>The development of sustainable electrode materials that allows sufficient accumulation of redox species is key to advancing biophotovoltaic (BPV) technologies. In this study, activated carbon derived from agave fiber was evaluated as an anodic electrode material for BPV devices. Activated carbon samples were synthesized at 600, 700, and 800 °C using different agave biomass-to-KOH impregnation ratios from 1:1 to 1:3. The resulting carbons were comprehensively characterized to analyze their morphology and composition, and to establish structure–performance relationships. Electrochemical evaluation using <em>Coelastrella</em> sp. biofilms revealed that the electrode produced at 700 °C with a 1:2 ratio delivered the best performance, achieving a specific capacity of 3.50 mAh/g. Both impregnation ratio and activation temperature influenced biofilm accumulation, while a balanced <em>meso</em>–micropore structure favored electron transfer from the bulk to the anode surface, ensuring sufficient interaction with photosynthetic microorganisms of <em>Coelastrella</em> sp. The maximum power density achieved was 1.3 mW/m<sup>2</sup>, with an open-circuit voltage of ∼140 mV at the lowest current density (∼6 mA/m<sup>2</sup>), and a short-circuit current density of ∼24 mA/m<sup>2</sup>. These findings demonstrate the potential of <em>Coelastrella</em> sp. biofilms to enhance the functionality of electrochemical materials by improving electron transfer kinetics, thereby contributing to the development of clean technologies that harness solar energy.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"95 ","pages":"Article 104594"},"PeriodicalIF":4.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147386337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Geography and seasonality as factors affecting lipid classes and anti-inflammatory activity of Rugulopteryx okamurae, an invasive seaweed in Southwest Europe 地理和季节因素对欧西南地区入侵海藻巨纹龙鸟脂类和抗炎活性的影响

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2026-04-01 Epub Date: 2026-03-03 DOI: 10.1016/j.algal.2026.104618

A.M. García Cervantes , M. Carmona-Fernández , A. Gomes-Bispo , C. Cardoso , C. Afonso , J.L. Guil-Guerrero , N.M. Bandarra

Rugulopteryx okamurae is an invasive brown seaweed originating in the Pacific Ocean that has spread to Southwestern European shores in the last decade, reaching high densities and causing large ecological and economic impacts. The lipid classes and anti-inflammatory activity of this seaweed species was investigated, comprising an analysis of different geographical locations in the Mediterranean Sea and Atlantic Ocean as well as winter and summer as growing seasons. The fatty acid (FA) composition of the biomass, main lipid classes, and cyclooxygenase-2 (COX-2) inhibition were analysed. There is evidence supporting a possible class-level lipid distribution pattern specific to R. okamurae, differentiating it from other brown seaweeds. The FA profile revealed prevalence of palmitic acid and saturated FA (SFA) in all lipid classes. Lipid class distribution in R. okamurae was characterized by a majority of polar lipids, with non-polar lipids making up to 40% of the total lipid content. Within polar lipids, glycolipids were the main class and monogalactosyldiacylglycerols (MGDG) had a share ranging from 45 to 58% of the total lipids. The SFA content ranged between 30.8 ± 1.3% (of total FA) in Atlantic (Algarve) R. okamurae MGDG and 82.5 ± 2.6% in Atlantic R. okamurae phospholipids. The FA profiles of R. okamurae from the two studied locations were quite similar, differing in palmitic acid content in triacylglycerols (TAG), oleic acid in MGDG, PUFA in digalactosyl diacylglycerols (DGDG), and n-3/n-6 ratio in MGDG. Regarding this latter parameter, the highest n-3/n-6 ratio was in Algarve MGDG samples, 1.8 ± 0.1. The preliminary screening of anti-inflammatory activity by the COX-2 assay showed higher levels of this parameter in summer R. okamurae (76.3 and 78.5% COX-2 inhibition). Therefore, R. okamurae exhibits distinctive traits and there is a potential valorization by component isolation (e.g. MGDG) and further refining of biologically active fractions (e.g. anti-inflammatory ethanol extracts).

okamurrugulopteryx okamurae是一种源自太平洋的入侵褐藻，在过去十年中已经扩散到欧洲西南部海岸，密度很高，造成了巨大的生态和经济影响。通过对地中海和大西洋不同地理位置以及生长季节冬季和夏季的分析，研究了这种海藻的脂类和抗炎活性。分析了生物质的脂肪酸组成、主要脂类以及对环氧合酶-2的抑制作用。有证据支持一种可能的类水平脂质分布模式，冈村褐藻与其他褐藻区别开来。脂肪酸谱显示棕榈酸和饱和脂肪酸（SFA）在所有脂类中普遍存在。冈村田鼠脂类分布以极性脂类为主，非极性脂类占总脂类含量的40%。在极性脂类中，糖脂类是主要的一类，单半乳糖二酰基甘油（MGDG）占总脂类的45% ~ 58%。大西洋（Algarve）冈村田鼠MGDG中SFA含量为30.8±1.3%，大西洋冈村田鼠磷脂中SFA含量为82.5±2.6%。两个研究地点的冈村田鼠的FA谱非常相似，只是三酰甘油（TAG）中的棕榈酸含量、MGDG中的油酸含量、双半乳糖二酰甘油（DGDG）中的PUFA含量和MGDG中的n-3/n-6比值不同。对于后一参数，Algarve MGDG样品的n-3/n-6比值最高，为1.8±0.1。通过COX-2实验初步筛选的抗炎活性显示，夏季okamurae的该参数水平较高（COX-2抑制率为76.3和78.5%）。因此，okamurae表现出独特的性状，并且通过分离组分（例如MGDG）和进一步精制生物活性组分（例如抗炎乙醇提取物）有潜在的价值。

{"title":"Geography and seasonality as factors affecting lipid classes and anti-inflammatory activity of Rugulopteryx okamurae, an invasive seaweed in Southwest Europe","authors":"A.M. García Cervantes , M. Carmona-Fernández , A. Gomes-Bispo , C. Cardoso , C. Afonso , J.L. Guil-Guerrero , N.M. Bandarra","doi":"10.1016/j.algal.2026.104618","DOIUrl":"10.1016/j.algal.2026.104618","url":null,"abstract":"<div><div><em>Rugulopteryx okamurae</em> is an invasive brown seaweed originating in the Pacific Ocean that has spread to Southwestern European shores in the last decade, reaching high densities and causing large ecological and economic impacts. The lipid classes and anti-inflammatory activity of this seaweed species was investigated, comprising an analysis of different geographical locations in the Mediterranean Sea and Atlantic Ocean as well as winter and summer as growing seasons. The fatty acid (FA) composition of the biomass, main lipid classes, and cyclooxygenase-2 (COX-2) inhibition were analysed. There is evidence supporting a possible class-level lipid distribution pattern specific to <em>R. okamurae</em>, differentiating it from other brown seaweeds. The FA profile revealed prevalence of palmitic acid and saturated FA (SFA) in all lipid classes. Lipid class distribution in <em>R. okamurae</em> was characterized by a majority of polar lipids, with non-polar lipids making up to 40% of the total lipid content. Within polar lipids, glycolipids were the main class and monogalactosyldiacylglycerols (MGDG) had a share ranging from 45 to 58% of the total lipids. The SFA content ranged between 30.8 ± 1.3% (of total FA) in Atlantic (Algarve) <em>R. okamurae</em> MGDG and 82.5 ± 2.6% in Atlantic <em>R. okamurae</em> phospholipids. The FA profiles of <em>R. okamurae</em> from the two studied locations were quite similar, differing in palmitic acid content in triacylglycerols (TAG), oleic acid in MGDG, PUFA in digalactosyl diacylglycerols (DGDG), and n-3/n-6 ratio in MGDG. Regarding this latter parameter, the highest n-3/n-6 ratio was in Algarve MGDG samples, 1.8 ± 0.1. The preliminary screening of anti-inflammatory activity by the COX-2 assay showed higher levels of this parameter in summer <em>R. okamurae</em> (76.3 and 78.5% COX-2 inhibition). Therefore, <em>R. okamurae</em> exhibits distinctive traits and there is a potential valorization by component isolation (e.g. MGDG) and further refining of biologically active fractions (e.g. anti-inflammatory ethanol extracts).</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"95 ","pages":"Article 104618"},"PeriodicalIF":4.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147386394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tracking lipid droplet dynamics using BODIPY C12 in Phaeodactylum tricornutum 利用BODIPY C12跟踪三角褐指藻脂滴动力学

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2026-04-01 Epub Date: 2026-03-03 DOI: 10.1016/j.algal.2026.104622

Ayushi Dalmia, Inna Khozin-Goldberg

Oleaginous microalgae are central to fundamental research on microalgae and biotechnology for single-cell oil production. Although many tools to manipulate physiological and molecular mechanisms have been developed for microalgae, cellular biology tools that enable visualization of lipid droplet (LD) dynamics in vivo are lacking. In this study, we demonstrate the use of the fluorescent fatty acid conjugate BODIPY C12 (BP C12) to study LD dynamics in two ecotypes of the model diatom Phaeodactylum tricornutum Pt1 and Pt4, as well as fatty acid trafficking in vivo, through real-time visualization of LD formation and breakdown by epifluorescence microscopy and FACS. Cells supplied with BP C12 under nutrient-replete conditions exhibited incorporation of BP C12 signal inside the cells in tiny vesicles. Under nitrogen starvation, the BP C12 signal was localized to the preformed LDs. During recovery from nitrogen starvation, we observed the breakdown of the LDs harbouring the BP C12 signal. Thin-layer chromatography showed that most BP C12 remained unesterified, but the signal was found in both polar and neutral lipids, suggesting its suitability for lipid-metabolic studies in P. tricornutum. Use of BP C12 also elucidated substrate utilization in the two ecotypes. These findings pave the way for the use of BP C12 in various assays with P. tricornutum.

产油微藻是微藻和单细胞产油生物技术基础研究的核心。尽管已经开发了许多工具来操纵微藻的生理和分子机制，但缺乏能够可视化脂滴（LD）体内动力学的细胞生物学工具。在本研究中，我们利用荧光脂肪酸偶联物BODIPY C12 （BP C12）通过荧光显微镜和FACS实时可视化LD的形成和分解，研究了两种生态型硅藻模型三角褐指藻Pt1和Pt4的LD动力学以及脂肪酸在体内的运输。在营养充足的条件下，提供BP C12的细胞显示BP C12信号在细胞内的小泡中结合。在氮饥饿条件下，BP C12信号被定位到预制的ld上。在氮饥饿恢复过程中，我们观察到含有BP C12信号的ld被破坏。薄层色谱显示，大部分BP C12未酯化，但在极性和中性脂质中均发现该信号，表明其适合于三角藻脂质代谢研究。BP C12的使用也阐明了两种生态型对底物的利用。这些发现为BP C12在三角锥虫的各种检测中使用铺平了道路。

{"title":"Tracking lipid droplet dynamics using BODIPY C12 in Phaeodactylum tricornutum","authors":"Ayushi Dalmia, Inna Khozin-Goldberg","doi":"10.1016/j.algal.2026.104622","DOIUrl":"10.1016/j.algal.2026.104622","url":null,"abstract":"<div><div>Oleaginous microalgae are central to fundamental research on microalgae and biotechnology for single-cell oil production. Although many tools to manipulate physiological and molecular mechanisms have been developed for microalgae, cellular biology tools that enable visualization of lipid droplet (LD) dynamics in vivo are lacking. In this study, we demonstrate the use of the fluorescent fatty acid conjugate BODIPY C12 (BP C12) to study LD dynamics in two ecotypes of the model diatom <em>Phaeodactylum tricornutum</em> Pt1 and Pt4, as well as fatty acid trafficking in vivo, through real-time visualization of LD formation and breakdown by epifluorescence microscopy and FACS. Cells supplied with BP C12 under nutrient-replete conditions exhibited incorporation of BP C12 signal inside the cells in tiny vesicles. Under nitrogen starvation, the BP C12 signal was localized to the preformed LDs. During recovery from nitrogen starvation, we observed the breakdown of the LDs harbouring the BP C12 signal. Thin-layer chromatography showed that most BP C12 remained unesterified, but the signal was found in both polar and neutral lipids, suggesting its suitability for lipid-metabolic studies in <em>P. tricornutum</em>. Use of BP C12 also elucidated substrate utilization in the two ecotypes. These findings pave the way for the use of BP C12 in various assays with <em>P. tricornutum</em>.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"95 ","pages":"Article 104622"},"PeriodicalIF":4.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147386395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated biorefinery of Limnospira (Arthrospira) platensis: Recycling cultivation medium and biosorption of endocrine disruptors from water 平滨螺（Arthrospira platensis）的综合生物炼制：培养基的循环利用和水中内分泌干扰物的生物吸附

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2026-04-01 Epub Date: 2026-02-27 DOI: 10.1016/j.algal.2026.104616

Alenne Prince Junqueira de Moraes , Camylle Guimarães Scheliga , Yollanda Carolina da Silva Ferreira Vançato , Cláudia Maria Luz Lapa Teixeira , André Luís de Sá Salomão

Microalgae-based systems are increasingly recognized as scalable platforms for producing high-value bioproducts while addressing water pollution, including emerging contaminants. However, large-scale production is often constrained by high operational costs. This study evaluated biomass production of the cyanobacterium Limnospira (Arthrospira) platensis over two consecutive cultivation cycles, with the culture medium from the first cycle recycled in the second. Mediumrecycling reduced freshwater and nutrient inputs without compromising biomass productivity. Biomass from both cycles exhibited high protein (59.8 ± 1.9% in cycle 1; 52.6 ± 2.0% in cycle 2) and carbohydrate contents (29.3 ± 1.8% and 39.0 ± 2.4%, respectively), supporting diverse valorization pathways. First-cycle biomass is suitable for high-value applications such as nutraceuticals, while second-cycle biomass was evaluated as a low-cost biosorbent for removing 17α-ethinylestradiol (EE2), a pseudo-persistent endocrinedisruptorfrequently found in aquatic ecosystems. Biosorption assays achieved ∼70% EE2 removal at 30 μg·L⁻¹ within 7 h using 0.6 g·L⁻¹ of biomass (biosorbent). Kinetics followed a pseudo-second-order model (R² = 0.98), and equilibrium data fitted the Freundlich isotherm, with a maximum adsorption capacity of 251.28 μg·g⁻¹. These results demonstrate that cultivation medium recycling can simultaneously reduce resource consumption and enable cascade biomass valorization, providing a scalable approach aligned with circular bioeconomy principles and sustainable tertiary wastewater treatment.

基于微藻的系统越来越被认为是生产高价值生物产品的可扩展平台，同时解决水污染，包括新出现的污染物。然而，大规模生产往往受到高运营成本的限制。本研究评估了两个连续培养周期的蓝藻Limnospira (Arthrospira) platensis的生物量产量，第一个循环的培养基在第二个循环中循环使用。中度循环减少了淡水和养分投入，但不影响生物量生产力。两个循环的生物量均表现出较高的蛋白质含量（第1周期为59.8±1.9%，第2周期为52.6±2.0%）和碳水化合物含量（分别为29.3±1.8%和39.0±2.4%），支持不同的增值途径。第一循环生物质适用于高价值应用，如营养品，而第二循环生物质被评价为一种低成本的生物吸附剂，用于去除17α-炔雌醇（EE2），这是一种在水生生态系统中经常发现的伪持久性内分泌干扰物。生物吸附试验使用0.6 g·L−1的生物质（生物吸附剂），在30 μg·L−1的条件下，在7小时内实现了~ 70%的EE2去除率。动力学符合拟二阶模型（R2 = 0.98），平衡数据符合Freundlich等温线，最大吸附量为251.28 μg·g−1。这些结果表明，培养基回收可以同时减少资源消耗和实现级联生物质增值，提供了一种符合循环生物经济原则和可持续三级废水处理的可扩展方法。

{"title":"Integrated biorefinery of Limnospira (Arthrospira) platensis: Recycling cultivation medium and biosorption of endocrine disruptors from water","authors":"Alenne Prince Junqueira de Moraes , Camylle Guimarães Scheliga , Yollanda Carolina da Silva Ferreira Vançato , Cláudia Maria Luz Lapa Teixeira , André Luís de Sá Salomão","doi":"10.1016/j.algal.2026.104616","DOIUrl":"10.1016/j.algal.2026.104616","url":null,"abstract":"<div><div>Microalgae-based systems are increasingly recognized as scalable platforms for producing high-value bioproducts while addressing water pollution, including emerging contaminants. However, large-scale production is often constrained by high operational costs. This study evaluated biomass production of the cyanobacterium <em>Limnospira (Arthrospira) platensis</em> over two consecutive cultivation cycles, with the culture medium from the first cycle recycled in the second. Mediumrecycling reduced freshwater and nutrient inputs without compromising biomass productivity. Biomass from both cycles exhibited high protein (59.8 ± 1.9% in cycle 1; 52.6 ± 2.0% in cycle 2) and carbohydrate contents (29.3 ± 1.8% and 39.0 ± 2.4%, respectively), supporting diverse valorization pathways. First-cycle biomass is suitable for high-value applications such as nutraceuticals, while second-cycle biomass was evaluated as a low-cost biosorbent for removing 17α-ethinylestradiol (EE2), a pseudo-persistent endocrinedisruptorfrequently found in aquatic ecosystems. Biosorption assays achieved ∼70% EE2 removal at 30 μg·L<sup>−1</sup> within 7 h using 0.6 g·L<sup>−1</sup> of biomass (biosorbent). Kinetics followed a pseudo-second-order model (R<sup>2</sup> = 0.98), and equilibrium data fitted the Freundlich isotherm, with a maximum adsorption capacity of 251.28 μg·g<sup>−1</sup>. These results demonstrate that cultivation medium recycling can simultaneously reduce resource consumption and enable cascade biomass valorization, providing a scalable approach aligned with circular bioeconomy principles and sustainable tertiary wastewater treatment.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"95 ","pages":"Article 104616"},"PeriodicalIF":4.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147386342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphology-dependent biotoxic effects and cellular damage mechanisms of silver nanoparticles on Chlorella vulgaris and Scenedesmus obliquus 银纳米颗粒对普通小球藻和斜小球藻的形态依赖性生物毒性效应及细胞损伤机制

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2026-04-01 Epub Date: 2026-02-24 DOI: 10.1016/j.algal.2026.104612

Wei Yu , Yongchang Shang , Zhenyu Wang , Yue Yang , Chenxi Zhang , Ningcan Deng , Kaixuan Wang , Ting Ma , Xiuzhen Yang , Miao Xu , Fan Mo , Haibo Li

The widespread application of silver nanoparticles (AgNPs) leads to their inevitable release into freshwater ecosystems, raising ecological concerns. The present study involved the synthesis of silver nanospheres (AgNSs), nanocubes (AgNCs), and nanoplates (AgPLs) through chemical synthesis methods. It also investigates the morphological-dependent biotoxicity of AgNPs at two distinct concentrations (0–5 mg L⁻¹ and 0–5000 ng L⁻¹) on two species of algae, C. vulgaris and S. obliquus. The results demonstrated that AgPLs exhibited the strongest inhibitory growth, with 0.5 mg L⁻¹ AgPLs inhibiting C. vulgaris growth by 58% (2.5–2.7-fold higher than AgNSs/AgNCs) and 5 mg L⁻¹ AgPLs inhibiting S. obliquus by 76%. Transmission electron microscopy (TEM) showed AgPLs caused cell content leakage, AgNCs induced starch granule accumulation, and AgNSs only damaged cell walls. Low-concentration (ng L⁻¹) AgNPs triggered a “low-dose stimulation, high-dose inhibition” hormesis on S. obliquus chlorophyll a. AgPLs had the highest colloidal stability (zeta potential: −23.07 mV), and cell-associated Ag accumulation was morphology- and species-dependent, with S. obliquus more sensitive than C. vulgaris. These findings provide toxicity data for AgNPs, supporting ecological risk assessments by incorporating morphology and species specificity.

银纳米颗粒（AgNPs）的广泛应用导致其不可避免地释放到淡水生态系统中，引起生态问题。本研究采用化学合成方法合成了银纳米球（AgNSs）、纳米立方（AgNCs）和纳米板（AgPLs）。它还研究了两种不同浓度（0-5 mg L - 1和0-5000 ng L - 1）的AgNPs对C. vulgaris和S. obliquus两种藻类的形态依赖性生物毒性。结果表明，AgPLs对C. vulgaris的抑制作用最强，0.5 mg L−1 AgPLs对C. vulgaris的抑制作用为58%（比AgNSs/AgNCs高2.5 - 2.7倍），5mg L−1 AgPLs对S. obliquus的抑制作用为76%。透射电镜（TEM）显示，AgPLs引起细胞内容物渗漏，AgNCs引起淀粉颗粒堆积，AgNSs仅破坏细胞壁。低浓度（ng L−1）AgNPs对斜叶参叶绿素a具有“低剂量刺激，高剂量抑制”的效应。AgNPs具有最高的胶体稳定性（zeta电位：- 23.07 mV），细胞相关的银积累具有形态和物种依赖性，斜叶参对银积累的敏感性高于普通叶参。这些发现提供了AgNPs的毒性数据，通过结合形态学和物种特异性来支持生态风险评估。

{"title":"Morphology-dependent biotoxic effects and cellular damage mechanisms of silver nanoparticles on Chlorella vulgaris and Scenedesmus obliquus","authors":"Wei Yu , Yongchang Shang , Zhenyu Wang , Yue Yang , Chenxi Zhang , Ningcan Deng , Kaixuan Wang , Ting Ma , Xiuzhen Yang , Miao Xu , Fan Mo , Haibo Li","doi":"10.1016/j.algal.2026.104612","DOIUrl":"10.1016/j.algal.2026.104612","url":null,"abstract":"<div><div>The widespread application of silver nanoparticles (AgNPs) leads to their inevitable release into freshwater ecosystems, raising ecological concerns. The present study involved the synthesis of silver nanospheres (AgNSs), nanocubes (AgNCs), and nanoplates (AgPLs) through chemical synthesis methods. It also investigates the morphological-dependent biotoxicity of AgNPs at two distinct concentrations (0–5 mg L<sup>−1</sup> and 0–5000 ng L<sup>−1</sup>) on two species of algae, <em>C. vulgaris</em> and <em>S. obliquus</em>. The results demonstrated that AgPLs exhibited the strongest inhibitory growth, with 0.5 mg L<sup>−1</sup> AgPLs inhibiting <em>C. vulgaris</em> growth by 58% (2.5–2.7-fold higher than AgNSs/AgNCs) and 5 mg L<sup>−1</sup> AgPLs inhibiting <em>S. obliquus</em> by 76%. Transmission electron microscopy (TEM) showed AgPLs caused cell content leakage, AgNCs induced starch granule accumulation, and AgNSs only damaged cell walls. Low-concentration (ng L<sup>−1</sup>) AgNPs triggered a “low-dose stimulation, high-dose inhibition” hormesis on <em>S. obliquus</em> chlorophyll <em>a</em>. AgPLs had the highest colloidal stability (zeta potential: −23.07 mV), and cell-associated Ag accumulation was morphology- and species-dependent, with <em>S. obliquus</em> more sensitive than <em>C. vulgaris</em>. These findings provide toxicity data for AgNPs, supporting ecological risk assessments by incorporating morphology and species specificity.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"95 ","pages":"Article 104612"},"PeriodicalIF":4.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147386484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biofilm organization and ECM remodeling in Trebouxia lynniae during colony growth and lichen symbiosis 水蛭群落生长和地衣共生过程中生物膜组织和ECM重塑

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2026-04-01 Epub Date: 2026-03-09 DOI: 10.1016/j.algal.2026.104637

César Daniel Bordenave , Ayelén Gazquez , Lucia Muggia , Francisco García-Breijo , Ángeles Calatayud , Eva Barreno , Pedro Carrasco

Lichen-forming microalgae are traditionally studied within their symbiotic context, yet growing evidence shows they also persist as free-living organisms, revealing ecological flexibility, physiological autonomy, and potential contributions to ecosystem resilience. De novo lichen formation — the establishment of a new thallus from free-living fungal and algal partners — depends on traits of these free-living photobionts, yet the mechanisms that enable partner recognition, spatial organization, and functional integration remain poorly characterized. Understanding how microalgae organize themselves outside the symbiosis is therefore crucial to uncover how these traits contribute to thallus development and symbiotic reassembly. This study explores whether biofilm traits in the model microalga Trebouxia lynniae, including abundant extracellular matrix production and morphological and physiological stratification, are developmentally coordinated and retained or modified within the lichen thallus formed with the mycobiont Ramalina farinacea. Using confocal microscopy, flow cytometry, and ultrastructural analyses, we show that T. lynniae forms structured, biofilm-like colonies with spatially stratified photosynthetic activity. Within the lichen thallus, vegetative algal cells maintain layered organization in repeating undulating patterns, yet extracellular matrix is markedly reduced or absent, suggesting a functional repurposing of extracellular polymeric substances from structural cohesion to fungal nutrition. Flow cytometry further indicates that most lichenized algal cells remain in a vegetative, non-reproductive state, consistent with suppression of photobiont sexual reproduction within the thallus. Our findings reveal that extracellular matrix remodeling and biofilm architecture are central to coordinating symbiotic integration in lichens, highlighting biofilm-like growth as an evolutionary step toward stable mutualism.

形成地衣的微藻传统上是在它们的共生环境中进行研究的，但越来越多的证据表明，它们也作为自由生物存在，揭示了生态灵活性、生理自主性和对生态系统恢复力的潜在贡献。新生地衣的形成——从自由生活的真菌和藻类伴侣中建立一个新的菌体——取决于这些自由生活的光生物的特征，然而，使伴侣识别、空间组织和功能整合的机制仍然不清楚。因此，了解微藻如何在共生之外组织自己对于揭示这些特征如何促进菌体发育和共生重组至关重要。本研究探讨了模式微藻（Trebouxia lynniae）的生物膜特征，包括丰富的细胞外基质生成和形态生理分层，是否在与真菌Ramalina farinacea形成的地衣菌体中发育协调并保留或修饰。利用共聚焦显微镜、流式细胞术和超微结构分析，我们发现T. lynniae形成结构化的、生物膜样菌落，具有空间分层的光合活性。在地衣菌体中，营养藻细胞以重复的波动模式维持层状组织，但细胞外基质明显减少或缺失，表明细胞外聚合物质从结构凝聚力到真菌营养的功能重新定位。流式细胞术进一步表明，大多数地衣化的藻类细胞仍处于营养、非生殖状态，这与抑制菌体内光生物有性生殖一致。我们的研究结果表明，细胞外基质重塑和生物膜结构是协调地衣共生整合的核心，强调了生物膜样生长是迈向稳定共生的进化步骤。

{"title":"Biofilm organization and ECM remodeling in Trebouxia lynniae during colony growth and lichen symbiosis","authors":"César Daniel Bordenave , Ayelén Gazquez , Lucia Muggia , Francisco García-Breijo , Ángeles Calatayud , Eva Barreno , Pedro Carrasco","doi":"10.1016/j.algal.2026.104637","DOIUrl":"10.1016/j.algal.2026.104637","url":null,"abstract":"<div><div>Lichen-forming microalgae are traditionally studied within their symbiotic context, yet growing evidence shows they also persist as free-living organisms, revealing ecological flexibility, physiological autonomy, and potential contributions to ecosystem resilience. <em>De novo</em> lichen formation — the establishment of a new thallus from free-living fungal and algal partners — depends on traits of these free-living photobionts, yet the mechanisms that enable partner recognition, spatial organization, and functional integration remain poorly characterized. Understanding how microalgae organize themselves outside the symbiosis is therefore crucial to uncover how these traits contribute to thallus development and symbiotic reassembly. This study explores whether biofilm traits in the model microalga <em>Trebouxia lynniae</em>, including abundant extracellular matrix production and morphological and physiological stratification, are developmentally coordinated and retained or modified within the lichen thallus formed with the mycobiont <em>Ramalina farinacea</em>. Using confocal microscopy, flow cytometry, and ultrastructural analyses, we show that <em>T. lynniae</em> forms structured, biofilm-like colonies with spatially stratified photosynthetic activity. Within the lichen thallus, vegetative algal cells maintain layered organization in repeating undulating patterns, yet extracellular matrix is markedly reduced or absent, suggesting a functional repurposing of extracellular polymeric substances from structural cohesion to fungal nutrition. Flow cytometry further indicates that most lichenized algal cells remain in a vegetative, non-reproductive state, consistent with suppression of photobiont sexual reproduction within the thallus. Our findings reveal that extracellular matrix remodeling and biofilm architecture are central to coordinating symbiotic integration in lichens, highlighting biofilm-like growth as an evolutionary step toward stable mutualism.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"95 ","pages":"Article 104637"},"PeriodicalIF":4.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147386550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Agrobacterium-mediated transformation of Chlamydomonas reinhardtii to produce recombinant human serum albumin 农杆菌介导的莱茵衣单胞菌转化制备重组人血清白蛋白

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2026-04-01 Epub Date: 2026-03-03 DOI: 10.1016/j.algal.2026.104619

Pratheesh Prakasam Thanka , Vineetha Mohan , Muraleedhara Kurup , Menatalla M. Ismail , Mallik Sudhanshu , Abdel-Hamid I. Mourad

Human serum albumin (HSA) constitutes the most significant fraction of proteins in human blood plasma. It remains one of the most widely used therapeutic biomolecules in modern medicine, with global demand exceeding 500 t annually. Limitations in plasma-derived HSA (pHSA), including supply shortages and the risk of pathogen transmission, have accelerated the pursuit of recombinant production strategies. This study demonstrates the first successful expression of structurally and functionally validated rHSA in Chlamydomonas reinhardtii through Agrobacterium-mediated nuclear transformation, highlighting the platform's novelty. Molecular analyses validated stable integration and transcriptional activity of the transgene, while expression levels reached up to 0.5% of total soluble protein. Structural and functional assessments confirmed that algal-derived rHSA closely resembles native pHSA, and cytotoxicity assays (MTT) verified its safety in human cell cultures. Taken together, the results highlight C. reinhardtii as an economically viable and scalable platform for sustainable biomanufacturing of high-value therapeutic proteins, making it an attractive alternative to traditional production sources.

人血清白蛋白（HSA）是人血浆中最重要的蛋白质成分。它仍然是现代医学中应用最广泛的治疗性生物分子之一，全球需求量每年超过500吨。血浆源性HSA （pHSA）的局限性，包括供应短缺和病原体传播的风险，加速了对重组生产策略的追求。本研究首次通过农杆菌介导的核转化在莱茵衣藻中成功表达了结构上和功能上经过验证的rHSA，突出了该平台的新颖性。分子分析证实了转基因的稳定整合和转录活性，而表达水平高达总可溶性蛋白的0.5%。结构和功能评估证实，藻类衍生的rHSA与天然的pHSA非常相似，细胞毒性试验（MTT）证实了其在人类细胞培养中的安全性。综上所述，这些结果突出了C. reinhardtii作为经济上可行且可扩展的高价值治疗性蛋白质可持续生物制造平台，使其成为传统生产来源的有吸引力的替代品。

{"title":"Agrobacterium-mediated transformation of Chlamydomonas reinhardtii to produce recombinant human serum albumin","authors":"Pratheesh Prakasam Thanka , Vineetha Mohan , Muraleedhara Kurup , Menatalla M. Ismail , Mallik Sudhanshu , Abdel-Hamid I. Mourad","doi":"10.1016/j.algal.2026.104619","DOIUrl":"10.1016/j.algal.2026.104619","url":null,"abstract":"<div><div>Human serum albumin (HSA) constitutes the most significant fraction of proteins in human blood plasma. It remains one of the most widely used therapeutic biomolecules in modern medicine, with global demand exceeding 500 t annually. Limitations in plasma-derived HSA (pHSA), including supply shortages and the risk of pathogen transmission, have accelerated the pursuit of recombinant production strategies. This study demonstrates the first successful expression of structurally and functionally validated rHSA in <em>Chlamydomonas reinhardtii</em> through <em>Agrobacterium</em>-mediated nuclear transformation, highlighting the platform's novelty. Molecular analyses validated stable integration and transcriptional activity of the transgene, while expression levels reached up to 0.5% of total soluble protein. Structural and functional assessments confirmed that algal-derived rHSA closely resembles native pHSA, and cytotoxicity assays (MTT) verified its safety in human cell cultures. Taken together, the results highlight <em>C. reinhardtii</em> as an economically viable and scalable platform for sustainable biomanufacturing of high-value therapeutic proteins, making it an attractive alternative to traditional production sources.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"95 ","pages":"Article 104619"},"PeriodicalIF":4.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147386334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioactive from Ulva lactuca and Ulva fasciata: Structural insights and mechanistic roles in colon cancer therapy 乳状Ulva和筋膜状Ulva的生物活性：在结肠癌治疗中的结构见解和机制作用

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2026-04-01 Epub Date: 2026-02-10 DOI: 10.1016/j.algal.2026.104568

Divya Vijayakumar

Colon cancer is one of the causes of the cancer-related morbidity and mortality on the global scale, and it is crucial to develop safe and effective therapy solutions. Marine green macroalgae, especially species of Ulva have emerged as important sources of structurally diverse bioactive macromolecules with anticancer potential. This review summarizes the existing available information regarding the structure, functionality, and translational use of green algal macromolecules in colon cancer management. Ulvans are sulfated heteropolysaccharides enriched with rhamnose and uronic acids that induce apoptosis, arrest cell cycle, and block oncogenic signaling pathways such as Wnt/b-catenin, PI3K/Akt/mTOR, or NF-kB. The peptides, polyphenols, and polyunsaturated fatty acids derived in Ulva have complementary effects, as they increase antioxidant defense, inhibit chronic inflammation, and manipulate immune responses. The structure-function relationships reveal the importance of sulfate replacement, molecular weight, and monosaccharide composition to define bioactivity. Although in vitro and in vivo experiments prove that selective, low-toxicity, cytotoxicity is restricted to colon cancer cells and not to normal tissue, there is limited clinical validation. Some of the major challenges involve low solubility, absence of standardized procedures of extraction, and inadequate pharmacokinetic characterization. This review highlights the therapeutic potential of green algal macromolecules, highlights translational challenges, and suggests future efforts on developing functional foods, nutraceuticals, and adjuvant therapies against colon cancer.

结肠癌是全球范围内癌症相关发病和死亡的原因之一，开发安全有效的治疗方案至关重要。海洋绿色巨藻，特别是藻属巨藻已成为具有抗癌潜力的结构多样的生物活性大分子的重要来源。本文综述了现有的关于绿藻大分子在结肠癌治疗中的结构、功能和转化应用的信息。Ulvans是一种富含鼠李糖和糖醛酸的硫酸化杂多糖，可诱导细胞凋亡，阻滞细胞周期，阻断Wnt/b-catenin、PI3K/Akt/mTOR或NF-kB等致癌信号通路。从Ulva中提取的多肽、多酚和多不饱和脂肪酸具有互补作用，因为它们可以增强抗氧化防御，抑制慢性炎症，并操纵免疫反应。结构-功能关系揭示了硫酸盐替代、分子量和单糖组成对确定生物活性的重要性。虽然体外和体内实验证明，选择性的、低毒性的细胞毒性仅限于结肠癌细胞，而不是正常组织，但临床验证有限。一些主要的挑战包括低溶解度，缺乏标准化的提取程序，以及不充分的药代动力学表征。这篇综述强调了绿藻大分子的治疗潜力，强调了转化的挑战，并提出了未来开发功能食品、营养食品和辅助治疗结肠癌的努力。

{"title":"Bioactive from Ulva lactuca and Ulva fasciata: Structural insights and mechanistic roles in colon cancer therapy","authors":"Divya Vijayakumar","doi":"10.1016/j.algal.2026.104568","DOIUrl":"10.1016/j.algal.2026.104568","url":null,"abstract":"<div><div>Colon cancer is one of the causes of the cancer-related morbidity and mortality on the global scale, and it is crucial to develop safe and effective therapy solutions. Marine green macroalgae, especially species of Ulva have emerged as important sources of structurally diverse bioactive macromolecules with anticancer potential. This review summarizes the existing available information regarding the structure, functionality, and translational use of green algal macromolecules in colon cancer management. Ulvans are sulfated heteropolysaccharides enriched with rhamnose and uronic acids that induce apoptosis, arrest cell cycle, and block oncogenic signaling pathways such as Wnt/b-catenin, PI3K/Akt/mTOR, or NF-kB. The peptides, polyphenols, and polyunsaturated fatty acids derived in Ulva have complementary effects, as they increase antioxidant defense, inhibit chronic inflammation, and manipulate immune responses. The structure-function relationships reveal the importance of sulfate replacement, molecular weight, and monosaccharide composition to define bioactivity. Although in vitro and in vivo experiments prove that selective, low-toxicity, cytotoxicity is restricted to colon cancer cells and not to normal tissue, there is limited clinical validation. Some of the major challenges involve low solubility, absence of standardized procedures of extraction, and inadequate pharmacokinetic characterization. This review highlights the therapeutic potential of green algal macromolecules, highlights translational challenges, and suggests future efforts on developing functional foods, nutraceuticals, and adjuvant therapies against colon cancer.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"95 ","pages":"Article 104568"},"PeriodicalIF":4.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147386398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

One-step purification process to obtain analytical grade phycoerythrin from Porphyridium sp. using hydrophobic interaction chromatography (HIC) 疏水相互作用色谱（HIC）一步法纯化卟啉中分析级藻红蛋白

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2026-04-01 Epub Date: 2026-02-15 DOI: 10.1016/j.algal.2026.104596

Alejandro Piera , Carlos Sainz-Urruela , Juan J. Espada , Victoria Morales , Gemma Vicente , Luis Fernando Bautista

Phycoerythrin (PE) is a highly valued protein pigment with notable properties in various applications, including human health, pharmaceuticals, cosmetics, and the biomedical industry. High-purity PE production schemes often involve several purification steps, which can be costly, result in yield losses, and potentially lack scalability. This study proposes a one-step purification process based on hydrophobic interaction chromatography (HIC) to produce analytical-grade PE from an ionic liquid (IL)-based crude extract from Porphyridium sp. The HIC approach effectively leveraged the ILs' ionic strength using different resins with varying hydrophobic ligands, ligand concentrations, and agarose matrix properties. The purity achieved is shown as a function of the recovered PE yield, enabling the selection of a suitable resin based on the required purity and recovery for the PE applications. The highest recovery yield within the analytical grade was obtained with Butyl-S Sepharose 6 Fast Flow resin (73.0%). In contrast, the highest PE purity was achieved using Butyl Sepharose High Performance resin, yielding a maximum value of 8.2, a 9.9-fold increase compared to the crude extract. This resin also produced high purity PE relative to phycocyanin (A₆₂₀/A₅₄₅ = 0.01), comparable to commercial PE standards. Furthermore, the purification process was also highly effective for extracts with reused ILs, an essential consideration for the sustainability of the process when working with this type of solvent. Ultimately, this study provides valuable insights into the behaviour of PE when purified using diverse hydrophobic resins, which can guide the selection of the most appropriate resin based on the desired purity and yield targets.

藻红蛋白（PE）是一种非常有价值的蛋白质色素，在人体健康、制药、化妆品和生物医学等领域有着广泛的应用。高纯度PE生产方案通常涉及几个纯化步骤，这可能会导致成本高昂、产量损失，并且可能缺乏可扩展性。本研究提出了一种基于疏水相互作用色谱（HIC）的一步纯化工艺，从卟啉属植物的离子液体（IL）为基础的粗提取物中生产分析级聚乙烯。HIC方法有效地利用了不同树脂的离子强度，这些树脂具有不同的疏水配体、配体浓度和琼脂糖基质性质。所获得的纯度显示为PE回收率的函数，从而可以根据PE应用所需的纯度和回收率选择合适的树脂。在分析级内，丁基- s Sepharose 6快流树脂的回收率最高（73.0%）。相比之下，使用丁基Sepharose高性能树脂获得的PE纯度最高，最大值为8.2，与粗提取物相比增加了9.9倍。与藻蓝蛋白相比，该树脂也能生产出高纯度的PE (A620/A545 = 0.01)，与商业PE标准相当。此外，该纯化过程对重复使用的il提取物也非常有效，这是使用这种类型的溶剂时该过程可持续性的重要考虑因素。最终，本研究为PE在使用不同疏水树脂纯化时的行为提供了有价值的见解，这可以指导根据所需纯度和产量目标选择最合适的树脂。

{"title":"One-step purification process to obtain analytical grade phycoerythrin from Porphyridium sp. using hydrophobic interaction chromatography (HIC)","authors":"Alejandro Piera , Carlos Sainz-Urruela , Juan J. Espada , Victoria Morales , Gemma Vicente , Luis Fernando Bautista","doi":"10.1016/j.algal.2026.104596","DOIUrl":"10.1016/j.algal.2026.104596","url":null,"abstract":"<div><div>Phycoerythrin (PE) is a highly valued protein pigment with notable properties in various applications, including human health, pharmaceuticals, cosmetics, and the biomedical industry. High-purity PE production schemes often involve several purification steps, which can be costly, result in yield losses, and potentially lack scalability. This study proposes a one-step purification process based on hydrophobic interaction chromatography (HIC) to produce analytical-grade PE from an ionic liquid (IL)-based crude extract from <em>Porphyridium</em> sp. The HIC approach effectively leveraged the ILs' ionic strength using different resins with varying hydrophobic ligands, ligand concentrations, and agarose matrix properties. The purity achieved is shown as a function of the recovered PE yield, enabling the selection of a suitable resin based on the required purity and recovery for the PE applications. The highest recovery yield within the analytical grade was obtained with Butyl-S Sepharose 6 Fast Flow resin (73.0%). In contrast, the highest PE purity was achieved using Butyl Sepharose High Performance resin, yielding a maximum value of 8.2, a 9.9-fold increase compared to the crude extract. This resin also produced high purity PE relative to phycocyanin (A<sub>620</sub>/A<sub>545</sub> = 0.01), comparable to commercial PE standards. Furthermore, the purification process was also highly effective for extracts with reused ILs, an essential consideration for the sustainability of the process when working with this type of solvent. Ultimately, this study provides valuable insights into the behaviour of PE when purified using diverse hydrophobic resins, which can guide the selection of the most appropriate resin based on the desired purity and yield targets.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"95 ","pages":"Article 104596"},"PeriodicalIF":4.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147386479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0