Algal Research-Biomass Biofuels and Bioproducts最新文献

{"title":"Does health information influence consumer perception of seaweed-containing foods? A repeated exposure study","authors":"Rachael Moss,&nbsp;Matthew Code,&nbsp;Brianna Power,&nbsp;Zach Adams,&nbsp;Mackenzie Gorman,&nbsp;Matthew B. McSweeney","doi":"10.1016/j.algal.2026.104587","DOIUrl":"10.1016/j.algal.2026.104587","url":null,"abstract":"<div><div>Seaweed has been proposed as a nutrient-dense, sustainable ingredient, but they are underutilized in the Western world due to undesirable sensory properties or unfamiliarity. One method to increase consumption, may be to educate consumers on the nutritional benefits of seaweed. Nutritional information has been found to have a positive influence on consumer perception of foods. Furthermore, familiarity with a product has been found to influence consumer acceptance. As such, this study explored how nutritional information and repeated exposure influenced consumer perception and acceptance of seaweed, on its own (dulse- <em>Palmaria palmata</em>) and when it is incorporated into a food product (bread with brown seaweed, <em>Ascophyllum nodosum</em>). Participants (n = 148) evaluated dulse and seaweed bread with and without nutritional information in two different testing sessions. Consumers evaluated both seaweed products for their perceived tastiness, healthiness, sustainability, acceptance, and sensory perception using check-all-that-apply (CATA). The results revealed the nutritional information had an influence on the tastiness, healthiness, and sustainability of the dulse, but only influenced the tastiness of the seaweed bread. The repeated exposure increased acceptance of the seaweed bread and decreased selection of negative attributes during the CATA task, when evaluating both the dulse and seaweed bread. The results identified that participants' evaluations of dulse (seaweed on its own) were strongly influenced by providing nutritional information. Future studies need to continue to explore how to nutritional information can be used to influence attitudes towards consumption of seaweed.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"94 ","pages":"Article 104587"},"PeriodicalIF":4.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146170355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of pretreatments used to obtain hydrolysates from microalgae biomass, and their effects on the recovery of carotenoids and fatty acids intended for agriculture applications","authors":"M.A. González-Cardoso ,&nbsp;R. Yepez ,&nbsp;E. Navarro-López ,&nbsp;A. Contreras-Gómez ,&nbsp;F.J. Alarcón-López ,&nbsp;M.C. Cerón-García","doi":"10.1016/j.algal.2025.104500","DOIUrl":"10.1016/j.algal.2025.104500","url":null,"abstract":"<div><div>Microalgae are considered a promising raw material for a variety of applications thanks to their nutritional profile. However, the rigid cell walls of certain species require pretreatments to facilitate the release of bioactive compounds. This study evaluated the impact of different pretreatments, including high-pressure homogenization (HPH), milling with alumina (M-AL), ultrasound (US), and microwave (MW), on protein hydrolysis and the recovery of fatty acids and carotenoids from the biomass of <em>Nannochloropsis gaditana</em>, <em>Isochrysis galbana</em> and <em>Arthrospira platensis</em>. All pretreatments significantly influenced protein hydrolysis. US at 80 % intensity resulted in the highest degree of hydrolysis (DH) for <em>I. galbana</em> (77 %), followed by <em>N. gaditana</em> (62 %). However, <em>A. platensis</em> showed no improvement compared to the control. Carotenoid recovery varied among species: the highest yield was achieved by <em>N. gaditana</em> (195 %) with US at 80 %, followed by <em>I. galbana</em> (180 %) with MW at 360 W, while the lowest recovery was observed in <em>A. platensis</em> (133 %) with HPH at 800 bar. Fatty acid yields also increased, with <em>N. gaditana</em> reaching 113 % (HPH at 800 bar) and 116 % (US at 80 %), and <em>I. galbana</em> reaching 112 % and 124 % under the same conditions. The highest recoveries in <em>A. platensis</em> were obtained with HPH at 400 and 800 bar (139 % and 135 %, respectively), compared to the untreated control. The bioactivity of the hydrolysates was assessed through the germination index of garden cress seeds. Only <em>I. galbana</em> hydrolysates enhanced germination, suggesting that specific pretreatments can improve the recovery of functional compounds from microalgae.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"94 ","pages":"Article 104500"},"PeriodicalIF":4.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145923280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long-term study shows cyanobacterial blooms reduce ciliate functional stability by altering network complexity","authors":"Mamun Abdullah Al ,&nbsp;Zijie Xu ,&nbsp;Jun Zuo ,&nbsp;Peng Xiao ,&nbsp;Yuanyuan Xue ,&nbsp;Shuzhen Li ,&nbsp;Xue Yan ,&nbsp;Yuanyuan Mo ,&nbsp;Qingyun Yan ,&nbsp;Henglong Xu ,&nbsp;Markus Majaneva ,&nbsp;Erik Jeppesen ,&nbsp;Jun Yang","doi":"10.1016/j.algal.2026.104534","DOIUrl":"10.1016/j.algal.2026.104534","url":null,"abstract":"<div><div>Ecological communities are normally composed of multiple interacting species. A fundamental interaction type is the trophic (feeding) interaction. As an example, bloom-forming cyanobacteria may impact the assembly and diversity of ciliate communities in waters. However, it is unclear to what extent cyanobacterial blooms – in the short and long term – affect the functional stability of ciliates and the complexity of the ciliate-cyanobacterium interdomain network. In this study, we analyzed nine years of high throughput sequence data of amplicons targeting the V9 region of the 18S rRNA gene of ciliates and the V3–V4 region of the 16S rRNA gene of cyanobacteria in two subtropical reservoirs. Our results demonstrated cyanobacterium (<em>Raphidiopsis</em>) bloom succession in terms of biomass –with three distinct bloom and two non-bloom periods over nine years. Similarly, the functional trait distribution of ciliate communities showed significant bloom-related variations and differences in functional composition, accounting for 43% and 52% of the total variation in the Shidou and Bantou reservoirs, respectively. The variation in functional composition of ciliate communities in the different bloom periods ranged from 15% to 96% in Shidou Reservoir and from 16% to 84% in Bantou Reservoir. Using network analyses, we found that the ciliate-cyanobacterium interdomain network exhibited a bloom-related temporal trajectory with complex modular associations. Influential analyses showed that some cyanobacterial nodes played an important role in network structure and complexity, accounting for 21.4% and 22.2% cyanobacterial influential nodes between bloom and non-bloom periods in Shidou and Bantou reservoirs, respectively. The variation in cyanobacterial nodes in the different bloom periods ranged from 14.7 to 32.6% in Shidou Reservoir and from 19.5% to 35.6% in Bantou Reservoir. Furthermore, the functional stability of ciliates and the robustness of the ciliate-cyanobacterium interdomain network were comparatively lower during bloom than non-bloom periods, indicating that cyanobacterial blooms had a significant negative impact on ciliate functional stability and ciliate-cyanobacterium interdomain network complexity as well. We also found a weaker influence of environmental factors during cyanobacterial bloom periods, and bloom-induced ecological changes were critical for microbial interactions and stability, highlighting the negative and strong impact of cyanobacterial blooms in freshwater ecosystems.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"94 ","pages":"Article 104534"},"PeriodicalIF":4.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147396800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect and mechanism of Synechococcus sp. PCC 7942 harboring VP26 in the immunity of Litopenaeus vannamei against WSSV","authors":"Xiang Ding ,&nbsp;Shengyu Liao ,&nbsp;Chan Zhu ,&nbsp;Xiaofeng Gao ,&nbsp;Hao Xing ,&nbsp;Rui Jia","doi":"10.1016/j.algal.2026.104532","DOIUrl":"10.1016/j.algal.2026.104532","url":null,"abstract":"<div><div>White spot syndrome virus (WSSV) has become one of the most widely spread deadly viruses in shrimp farming because of its wide host range, fast spreading speed, and high lethality. <em>Synechococcus</em> sp. PCC 7942 is a safe, edible, noncytotoxic cyanobacterium and is a good expression vector. As a tegument protein of WSSV, VP26 plays an important role in the stage of virus reproduction. This study is mainly about constructing <em>Synechococcus</em> sp. PCC 7942 harboring VP26, as well as detecting the expression of host immune pathway marker genes (Dorsal, Relish, STAT, c-Jun, and ALF1), metabolic enzyme activities including lysozyme (LZM), superoxide dismutase (SOD), phenoloxidase (PO), catalase (CAT), glutamate dehydrogenase (GDH), and fatty acid synthase (FAS), and mortality in vivo to evaluate its prevention and treatment effect on WSSV. The study showed that the oral administration of transformed <em>Synechococcus</em> sp. PCC 7942 followed by the challenge led to a relative survival rate of 80% compared with 0% of the positive group. The immune pathway marker gene expression levels and activity of 5 metabolic enzymes tested in the experiment group and the positive group showed an upward trend and reached a peak at 12 h, after which they decreased within 12–24 h. Nevertheless, the activity of LZM in these groups increased within 0–6 h, peaked at 6 h, then decreased. In all the expression and enzyme activity tests, the results in the experiment group were lower than those in the positive group. These results indicated that <em>Synechococcus</em> sp. PCC 7942 harboring VP26 had a positive effect on protecting shrimp from WSSV.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"94 ","pages":"Article 104532"},"PeriodicalIF":4.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146023291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acetate-enabled staged strategy in Chlorella vulgaris culture: Relieving ammonium inhibition while reducing ammonia stripping and enhancing protein content","authors":"Zhongliang Sun,&nbsp;Chenmei Bo,&nbsp;Shuonan Cao,&nbsp;Liqin Sun","doi":"10.1016/j.algal.2026.104527","DOIUrl":"10.1016/j.algal.2026.104527","url":null,"abstract":"<div><div>High-ammonium dosing effectively suppresses protozoan contamination in <em>Chlorella</em> cultivation but causes growth inhibition, ammonia volatilization, and photosynthetic damage. Developing a sustainable strategy that mitigates these adverse effects while maintaining productivity is critical for large-scale applications. A staged cultivation strategy combining ammonium bicarbonate treatment with acetate-based recovery successfully restored algal growth and nitrogen utilization. Acetate maintained near-neutral pH, reduced ammonia stripping by 41%, and increased biomass productivity by 92% compared with CO₂ control. Chlorophyll <em>a</em> fluorescence revealed faster recovery of <em>F</em>_<em>v</em><em>/F</em>_<em>m</em> and ψ₀, indicating enhanced PSII function and photochemical efficiency. Acetate alleviates ammonium inhibition through physicochemical buffering and metabolic coordination, stabilizing pH, supplying carbon skeletons for nitrogen assimilation, and supporting thylakoid repair. This dual-function approach provides a simple, cost-effective, and scalable solution for open-pond cultivation, integrating contamination control with improved nitrogen-use efficiency and photosynthetic resilience.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"94 ","pages":"Article 104527"},"PeriodicalIF":4.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145973856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel microalgae from Panax ginseng rhizosphere: a potential sustainable source of saponins and antioxidants","authors":"Yuqing He ,&nbsp;Jielin Wei ,&nbsp;Mengmeng Zhang ,&nbsp;Shiqi Xu ,&nbsp;Kexin Pei ,&nbsp;Mingyan Liu ,&nbsp;Chunxia Ma ,&nbsp;Haiyan Pei","doi":"10.1016/j.algal.2026.104549","DOIUrl":"10.1016/j.algal.2026.104549","url":null,"abstract":"<div><div>As an important medicinal plant, <em>Panax ginseng</em> harbours microorganisms in its rhizosphere with specific pharmaceutical values. However, few studies have focused specifically on the microalgae distributed in medicinal plants. In this study, we isolated and characterized four microalgal strains — <em>Asterarcys</em> sp. FDA-9, <em>Coelastrum</em> sp. FDA-10, <em>Coelastrella</em> sp. FDA-11, and <em>Klebsormidium</em> sp. FDA-14 — from the ginseng rhizosphere. A comparative analysis with ginseng revealed that these microalgae exhibited superior biomass productivity and synthesized valuable biochemicals, notably high levels of polyunsaturated fatty acids (&gt;40%). FDA-10 achieved the highest biomass (5.10 g L⁻¹), and content of carbohydrates (37.62% of dry mass DM), lipids (36.69% DM), and proteins (19.00% DM). The methanol extracts (ME) and crude polysaccharides (CP) from microalgae demonstrated antioxidant activity comparable to or even stronger than that of ginseng extracts. The DPPH scavenging rates of ME and CP from FDA-14 in 2.0 mg mL⁻¹ were respectively 2.20 times and 6.70 times higher than those in ginseng. Crucially, the crude saponin contents in the four microalgae (24.03–71.53 mg g⁻¹) significantly exceeded that of ginseng (12.06 mg g⁻¹), and untargeted metabolomic analysis revealed the presence of ginsenosides in the isolated microalgae. Our findings reveal these ginseng rhizosphere-derived microalgae to be very promising sustainable cell factories for the rapid production of pharmaceutically valuable bioactive compounds, such as saponins and antioxidants.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"94 ","pages":"Article 104549"},"PeriodicalIF":4.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146170300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a scalable design for biofuel production from green macroalgae hydrolysates","authors":"Hayet Djelal ,&nbsp;Alaa Salma ,&nbsp;Walaa Sayed ,&nbsp;Audrey Cabrol ,&nbsp;Maud Benoit ,&nbsp;Amance Corat ,&nbsp;Abdeltif Amrane ,&nbsp;Ronan Pierre","doi":"10.1016/j.algal.2025.104487","DOIUrl":"10.1016/j.algal.2025.104487","url":null,"abstract":"<div><div>This study investigated the valorization of green macroalgae in ethanol by the yeast <em>Saccharomyces cerevisiae</em>. The proof of concept was conducted with two green macroalgae hydrolysates, namely <em>Ulva rigida and Chaetomorpha linum</em> and the fermentation process was scaled-up from 250 mL and 500 mL shake flasks to a 3 L stirred-tank bioreactor. Firstly, the experiments used a synthetic medium based on algal hydrolysate, examining factors like nitrogen source, inoculum size, and salt content. After 72 h, ethanol yields were similar across the systems: 0.43 g.g⁻¹ in the bioreactor, compared to 0.46 and 0.45 g.g⁻¹ in 250 mL and 500 mL flasks, respectively. Secondly, fermenting green algae hydrolysate was tested, under similar conditions (without aeration and pH adjustment, with nitrogen enrichment, and 1 % <em>v</em>/v inoculum) resulted in an ethanol concentration of 9.05 g.L⁻¹, a yield of 0.44 g.g⁻¹, and 85.86 % efficiency. Thirdly, operating the process at larger scale was carried out on pilot scale of 77 L with <em>Chaetomorpha linum</em> hydrolysate. Glucose was fully consumed in 9 h, and maximum ethanol production (5.61 g.L⁻¹) occurred after 11 h, with a yield of 0.52 g.g⁻¹ which corresponds to the theoretical yield.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"94 ","pages":"Article 104487"},"PeriodicalIF":4.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145923282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insights into leveraging alga–bacterium cooperation for dairy wastewater valorization","authors":"Caterina Isabel Manzano-Puga ,&nbsp;Nuria Membrives-Barea ,&nbsp;Aitor Gómez-Osuna ,&nbsp;María Jesús Torres ,&nbsp;Alexandra Dubini ,&nbsp;David González-Ballester","doi":"10.1016/j.algal.2025.104481","DOIUrl":"10.1016/j.algal.2025.104481","url":null,"abstract":"<div><div>The integration of microalgae-bacteria consortia into wastewater treatment offers a promising strategy for sustainable biomass production and bioremediation. This study investigates the performance of <em>Chlamydomonas reinhardtii</em> in consortium with <em>Serratia liquefaciens</em> DWW128, isolated from raw dairy wastewater (DWW). The consortium, and respective monocultures were cultivated in both untreated, raw DWW and sterile, simulated DWW (sDWW). <em>C. reinhardtii</em> alone exhibited negligible growth in sDWW, while the native microbial community of raw DWW supported its growth (35.2 μg·mL⁻¹ chlorophyll and 1.6 g·L⁻¹ of biomass). However, native microorganisms also negatively affect the alga by limiting its maximum growth compared to cocultures with <em>S. liquefaciens</em> in raw DWW (53.2 μg·mL⁻¹ chlorophyll, 3.1 g·L⁻¹ biomass) and in sDWW (90 μg·mL⁻¹ chlorophyll, 7.1 g·L⁻¹ biomass). The enhancement of algal growth is more pronounced under aerobic conditions, and the supply of ammonium through bacterial proteolytic activity, and possibly also acetic acid, are key factors for algal growth. Cocultures can reduce up to 57.6 % of the bacterial CO₂ emissions. In contrast, <em>S. liquefaciens</em> did not depend on <em>C. reinhardtii</em> to grow successfully in DWW and can produce a substantial amount of bioH₂ (200.7 mL·L⁻¹) in 48 h. However, the presence of the alga enhances the bacterium's viability and persistence. This study provides valuable insights into harnessing the metabolic specialization of diverse microalgal and bacterial species to design tailored consortia capable of exploiting the broad spectrum of nutrient sources in wastewater, enabling more robust and effective biotechnological applications.</div><div><strong>Raw Dataset repository</strong>: <span><span>10.17632/39ckdr8w2n.1</span><svg><path></path></svg></span></div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"94 ","pages":"Article 104481"},"PeriodicalIF":4.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145923305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Co-utilization of bread waste and harmful algal bloom-derived cyanobacterial biomass for Euglena gracilis cultivation: Turning waste and pollution into resources","authors":"Woo Shik Jung ,&nbsp;Yugeun Jung ,&nbsp;Jee Young Kim ,&nbsp;Eun Hee Bae ,&nbsp;Yoon-E Choi","doi":"10.1016/j.algal.2025.104510","DOIUrl":"10.1016/j.algal.2025.104510","url":null,"abstract":"<div><div><em>Microcystis aeruginosa</em> is a harmful algal bloom (HAB)-forming cyanobacterium that poses significant environmental risks. Additionally, a large amount of bread waste rich in valuable carbon is discarded worldwide, resulting in both environmental and economic challenges. In this study, we sought to utilise <em>M. aeruginosa</em> biomass and bread waste for the cultivation of the beneficial microalga <em>Euglena gracilis</em>, thereby converting both HAB biomass and food waste into valuable resources. To this end, algal hydrolysate (AH) derived from <em>M. aeruginosa</em> and bread waste hydrolysate (BWH) were incorporated as components of an algal culture medium. Various proportions of AH were mixed with BWH, and the productivity of <em>E. gracilis</em> was assessed. Among all tested conditions, the 20 % AH medium resulted in the highest dry cell weight (9.01 g) of <em>E. gracilis</em>. During the subsequent bioreactor scale-up, biomass accumulation further increased to 9.87 g. Metabolite production was then compared under aerobic and anaerobic conditions using both AH and BWH based media. When cultivation transitioned from aerobic to anaerobic conditions, the lipid content of <em>E. gracilis</em> biomass increased from 12.2 % to 26.6 %. Furthermore, the fatty acid profile shifted toward a composition more suitable for jet fuel applications. This study presents a novel strategy demonstrating that both environmental pollutants (e.g., AH derived from HABs) and food by-products or waste (e.g., BWH) can serve as effective nutrient sources for microalgal cultivation, offering a promising approach for more economical and environmentally sustainable microalgae-based biotechnologies.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"94 ","pages":"Article 104510"},"PeriodicalIF":4.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145923350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Employing exopolysaccharide-rich cyanobacterial materials for microbial preservation","authors":"Xiaolong Yuan ,&nbsp;Tao Zheng ,&nbsp;Zhengke Li ,&nbsp;Wanxia Xia ,&nbsp;Xiang Gao","doi":"10.1016/j.algal.2026.104590","DOIUrl":"10.1016/j.algal.2026.104590","url":null,"abstract":"<div><div>Developing new microbial preservation technologies can advance existing approaches. Macroscopic cyanobacteria such as <em>Nostoc flagelliforme</em>, which are rich in exopolysaccharides, may hold potential for this application. This study investigated sterilized non-treated, methanol-treated, and acetone-treated <em>N. flagelliforme</em> materials for bacterial dry preservation. Methanol or acetone treatment disrupts cells while preserving polysaccharide components, primarily cell wall polysaccharides and exopolysaccharides. The physicochemical properties of the three materials were characterized via X-ray diffraction, X-ray photoelectron spectroscopy, and Fourier transform infrared spectroscopy. All three materials exhibited crystalline structures and specific functional groups; methanol and acetone treatments altered their surface elemental composition and functional groups. All of them could effectively preserved bacterial cells during dry storage at room temperature, with a survival rate of &gt;75% after 360 days. The acetone-treated <em>N. flagelliforme</em> material exhibited the best performance (83.2% survival rate). Bacterial viability was additionally verified through microscopic observation of fluorescence-stained cells. Scanning electron microscopy observations revealed that bacterial cells were bound to or embedded within the surface polysaccharide matrix. This study highlights natural exopolysaccharide-rich biomaterials as a low-cost, equipment-free alternative for microbial dry preservation.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"94 ","pages":"Article 104590"},"PeriodicalIF":4.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146170288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}