Algal Research-Biomass Biofuels and Bioproducts最新文献_第10页

Integrating a pilot scale Spirulina biorefinery into a local bioeconomy: A comparative life cycle assessment of environmental mitigation strategies 将试点规模的螺旋藻生物精炼厂纳入当地生物经济：环境缓解战略的生命周期比较评估

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2025-11-12 DOI: 10.1016/j.algal.2025.104434

L. Braud , K. McDonnell , F. Murphy

The use of energy and inorganic fertilisers have been identified as major contributors to the overall environmental impacts of algal value chains. This life cycle assessment (LCA) study compares three mitigation strategies aimed at reducing the environmental impacts of a novel European pilot scale Spirulina biorefinery and focusing on energy consumption, nutrient use, and transportation distances: (1) coupling algae cultivation with an anaerobic digestion and combined heat and power (AD-CHP) plant, (2) co-locating all biorefinery process stages, and (3) using photovoltaic (PV) panels to provide energy for biomass processing. While integrating the biorefinery with an AD-CHP plant initially appeared promising, the use of heat, electricity, and organic nitrogen led to increased climate change impacts and agricultural land occupation. This was primarily due to decreased Spirulina biomass productivity and the production of grass silage for co-digestion with cattle slurry. In contrast, the combined application of mitigation strategies (2) and (3), i.e. the co-location of all biorefinery processes and use of PV panels proved most effects, achieving 22% reduction in climate impacts. Solar energy use alone reduced climate impacts by 12% without shifting burdens to agricultural land occupation. The combined application of all strategies led to a 5% reduction in climate impacts. The sensitivity analysis highlighted the influence of the method used to handle multifunctionality on the LCA results. Overall, this study underscores the potential of integrating Spirulina biorefineries into a local bioeconomy and emphasises the significant role of renewable energy in reducing environmental impacts. These findings provide valuable insights to scale-up sustainable algae systems in Europe and advance the blue bioeconomy.

能源和无机肥料的使用已被确定为藻类价值链整体环境影响的主要因素。这项生命周期评估（LCA）研究比较了三种缓解策略，旨在减少欧洲新型中试规模螺旋藻生物炼制的环境影响，并关注能源消耗、营养物质利用和运输距离：(1)将藻类培养与厌氧消化和热电联产（AD-CHP）工厂结合起来，(2)将所有生物炼制过程阶段放在一起，(3)使用光伏（PV）板为生物质加工提供能源。虽然将生物精炼厂与AD-CHP工厂整合在一起最初看起来很有希望，但热、电和有机氮的使用导致气候变化影响和农业用地占用增加。这主要是由于螺旋藻生物量生产力下降和用于与牛浆共消化的草青贮的生产。相比之下，缓解战略(2)和(3)的联合应用，即所有生物炼制工艺和使用光伏电池板的共同选址，证明了最有效的效果，将气候影响减少了22%。仅太阳能的使用就减少了12%的气候影响，而没有将负担转移到农业用地上。所有战略的综合应用使气候影响减少了5%。敏感性分析突出了用于处理多功能性的方法对LCA结果的影响。总的来说，这项研究强调了将螺旋藻生物精炼厂整合到当地生物经济中的潜力，并强调了可再生能源在减少环境影响方面的重要作用。这些发现为扩大欧洲可持续藻类系统和推进蓝色生物经济提供了有价值的见解。

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引用次数: 0

Structural characterizations and anticoagulant activities of hydrolyzed low-molecular-weight arabinan-type sulfated polysaccharide from Chaetomorpha linum 毛茛低分子量阿拉伯型硫酸酸化水解多糖的结构表征及抗凝血活性

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2025-11-12 DOI: 10.1016/j.algal.2025.104428

Ling Qin , Xiao Chu , Ying Wang , Yuanqin Sun , Xiao Li , Wei Wang , Tianhong Liu

Sulfated polysaccharides from algae show significant promise for use in medication development and biomaterials. A sulfated rhamnogalactoarabinan, designated CHS1, was isolated from the green alga Chaetomorpha linum. Structural characterization revealed its backbone consists of →4)-β-L-Arap-(1 → residues, partially sulfated at C-2/C-3 and branched at C-3. Controlled acid degradation of CHS1 yielded seven low-molecular-weight (LMW) fractions, which maintained similar chemical structures to the native polysaccharide. The molecular weights of CHS1 and its LMW fractions were 680 kDa, 358 kDa, 247 kDa, 228 kDa, 175 kDa, 62 kDa, 31 kDa, and 9.3 kDa, respectively. In vitro anticoagulant assays demonstrated that CHS1 and its LMW fractions significantly prolonged activated partial thromboplastin time (APTT) and thrombin time (TT) in a concentration-dependent manner. As molecular weight decreases, the prolongation effects on APTT and TT correspondingly weaken, with longer chains exhibiting superior thrombin inhibitory activity. Both CHS1 and CHS1-L5 also displayed potent anticoagulant activity in vivo and effective thrombolytic activity in vitro, which was independent of molecular weight. These findings highlight the structure-dependent anticoagulant activity of sulfated rhamnogalactoarabinans and the potential of marine-derived sulfated rhamnogalactoarabinans as a novel source for antithrombotic biological products development.

从藻类中提取的硫酸多糖在药物开发和生物材料方面具有重要的应用前景。从毛藻（Chaetomorpha linum）中分离到硫酸酸化鼠李糖半乳糖阿拉伯糖（CHS1）。结构表征表明其主链由→4)-β- l - arap -(1)→残基组成，在C-2/C-3处部分硫酸化，在C-3处支链化。控制酸降解CHS1得到7个低分子量（LMW）馏分，这些馏分保持了与天然多糖相似的化学结构。CHS1及其LMW组分分子量分别为680 kDa、358 kDa、247 kDa、228 kDa、175 kDa、62 kDa、31 kDa和9.3 kDa。体外抗凝试验表明，CHS1及其LMW组分显著延长了活化的部分凝血活素时间（APTT）和凝血酶时间（TT），并呈浓度依赖性。随着分子量的减小，对APTT和TT的延长作用相应减弱，较长的链表现出更好的凝血酶抑制活性。CHS1和CHS1- l5在体内也表现出有效的抗凝血活性和体外有效的溶栓活性，且与分子量无关。这些发现强调了硫酸鼠李糖半乳糖阿拉伯糖的结构依赖性抗凝活性，以及海洋来源的硫酸鼠李糖半乳糖阿拉伯糖作为抗血栓生物制品开发的新来源的潜力。

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引用次数: 0

Sustainable integration of aquaculture wastewater treatment using Chlorococcum sp. in photobioreactor systems: Evaluation of nutrient removal, biomass productivity, and operational performance 在光生物反应器系统中使用绿球藻可持续整合水产养殖废水处理：营养物去除、生物量生产力和操作性能的评估

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2025-11-12 DOI: 10.1016/j.algal.2025.104427

Zubair Hashmi , Ibrahim Maina Idriss , Rieza Zulrian Aldio , Nur Aqidah Donglah , Juliana Zaini , Yusuf Wibisono , Muhammad Roil Bilad

Aquaculture growth intensifies challenges related to nutrient discharge, water use, and energy demand. This study evaluated three systems: traditional, batchwise wastewater treatment, and a membrane photobioreactor (MPBR) integrated recirculating aquaculture system, using environmental, economic, and technical metrics. The MPBR achieved the highest overall performance, with 87 % nitrate, 76 % phosphate, 82 % COD removal, 90 % water reuse, and the lowest water footprint (16.3 L/kg fish). At a 10-reactor scale, it produced 39.3 kg fish and 1.91 kg algal biomass annually, generating $577.46/year revenue and optimizing land use (0.243 m²/kg biomass). Integrating solar illumination eliminated lighting costs, reduced net CO₂ emissions by 1.68 %, and energy use by 1.7 %. Price elasticities were not uniformly low: MPBR/batchwise were resilient to fish price shocks (0.22–0.34) and moderately sensitive to algae price (0.66–0.78), whereas the traditional system remained highly fish-price sensitive (1.00); electricity and feed sensitivities were low (≈0.16, ≈0.03), with labor costs the principal vulnerability (≈0.8–0.92). Despite higher energy demand, the MPBR excelled in yield, resource recovery, and sustainability, offering a scalable solution aligned with circular bioeconomy and food–energy–water security goals.

水产养殖的增长加剧了与养分排放、用水和能源需求相关的挑战。本研究使用环境、经济和技术指标评估了三种系统：传统的分批废水处理系统和膜光生物反应器（MPBR）集成循环水养殖系统。MPBR的总体性能最高，硝酸盐含量为87%，磷酸盐含量为76%，COD去除率为82%，水回用率为90%，水足迹最低（16.3 L/kg鱼）。在10个反应器规模下，它每年生产39.3公斤鱼和1.91公斤藻类生物量，每年产生577.46美元的收入，并优化土地利用（0.243平方米/公斤生物量）。集成太阳能照明消除了照明成本，减少了1.68%的二氧化碳净排放量，减少了1.7%的能源使用。价格弹性并非一律低：MPBR/批次对鱼类价格冲击具有弹性（0.22-0.34），对藻类价格中等敏感（0.66-0.78），而传统系统对鱼类价格仍然高度敏感（1.00）；电力和饲料敏感性较低（≈0.16,≈0.03），人工成本是主要的脆弱性（≈0.8-0.92）。尽管能源需求增加，但MPBR在产量、资源回收和可持续性方面表现出色，提供了符合循环生物经济和粮食-能源-水安全目标的可扩展解决方案。

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引用次数: 0

Magnetite nanoparticles for microalgal biomass dewatering: A study on Chlorella sp. 磁性纳米颗粒用于微藻生物质脱水：小球藻的研究。

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2025-11-12 DOI: 10.1016/j.algal.2025.104431

Gloria Amo-Duodu , Houda Ennaceri , Parisa A. Bahri , Navid Reza Moheimani

Magnetic flocculation using magnetite nanoparticles (MNPs) has emerged as a promising technique for microalgae harvesting and dewatering, offering advantages over conventional methods. This study evaluated the key factors influencing the efficacy of magnetic harvesting of Chlorella sp. using commercial MNPs in two comparisons with pH-adjustment-sonication-extraction and pH-adjustment-sonication recovery methods for testing the recyclability of MNPs. Results showed that magnetic harvesting efficiencies exceeding 99 % were achieved within the pH range of 7–9 when MNPs were applied at concentrations between 5 and 10 g/L. From pH 7–9, there was no statistically significant variability in performance. In contrast, MNP concentration had a more pronounced effect: less than 50 % harvesting efficiency was observed at 0.1–0.3 g/L, whereas increasing the concentration to 0.5–1 g/L resulted in 70–90 % efficiency. To enable MNPs recycling, pH adjustment—with and without ultrasonication—and solvent extraction were tested and compared. The pH-only method enabled up to two reuse cycles with less than a 20 % reduction in efficiency, whereas the combined method extended reuse to three cycles but involved higher chemical usage and a greater risk of cell damage. These findings suggest that the pH-only detachment approach can be a more cost-effective and sustainable method for achieving efficient (>99 %) harvesting of Chlorella sp., particularly at neutral to alkaline pH levels. Additionally, this approach enables the repurposing of spent MNPs in applications such as soil and environmental remediation, offering potential to add value to waste materials and contribute to a more sustainable future.

利用磁铁矿纳米颗粒（MNPs）进行磁絮凝是一种很有前途的微藻收获和脱水技术，具有传统方法无法达到的优点。通过ph调节-超声提取和ph调节-超声回收两种方法的比较，研究了影响商业MNPs对小球藻磁性收获效果的关键因素，以检验MNPs的可回收性。结果表明，当MNPs的浓度为5 ~ 10 g/L时，在pH为7 ~ 9的范围内，磁性收获效率超过99%。从pH值7-9，性能没有统计学上显著的变化。相比之下，MNP浓度的效果更为明显：在0.1-0.3 g/L时，收获效率不到50%，而当MNP浓度增加到0.5-1 g/L时，收获效率达到70 - 90%。为了使MNPs回收利用，对pH调节（有和没有超声波）和溶剂萃取进行了测试和比较。仅使用ph值的方法可以重复使用两次，效率降低不到20%，而结合使用的方法可以重复使用三次，但涉及到更高的化学品使用量和更大的细胞损伤风险。这些发现表明，纯pH分离方法对于实现小球藻的高效收获（> 99%）是一种更具成本效益和可持续性的方法，特别是在中性至碱性pH水平下。此外，这种方法使废MNPs能够在土壤和环境修复等应用中重新利用，有可能增加废物的价值，并为更可持续的未来做出贡献。

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引用次数: 0

Marine macroalgal bioactives for wound healing: Molecular mechanisms, synergistic approaches, and biomaterial integration 伤口愈合的海洋大藻生物活性物质：分子机制、协同方法和生物材料整合

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2025-11-11 DOI: 10.1016/j.algal.2025.104422

Shruti S. Gawde, Maushmi S. Kumar

Wound healing is a dynamic biological process involving haemostasis, inflammation, proliferation, and tissue remodelling. Disruption of these phases in chronic wounds leads to prolonged inflammation, oxidative stress, and microbial infections, posing significant clinical challenges. Marine macroalgae are a promising source of bioactive compounds, including sulfated polysaccharides (fucoidan, carrageenan, alginate), polyphenols (phlorotannins), pigments (fucoxanthin), fatty acids, and sterols, that exhibit antioxidant, anti-inflammatory, antimicrobial, and cell-regenerative properties essential for wound repair. These compounds modulate key molecular pathways such as NF-κB, MAPK, COX-2, and Nrf2 to reduce oxidative damage, suppress pro-inflammatory cytokines, and inhibit biofilm-forming pathogens. Recent advances demonstrate the incorporation of seaweed-derived bioactives into hydrogels, nanocomposites, and scaffolds, improving wound closure, drug delivery, and synergistic interactions with conventional treatments. This review summarizes the pharmacological mechanisms of algal bioactives, explores their integration with advanced biomaterials, and highlights synergistic approaches that enhance therapeutic outcomes. In this review we have constructed a synergistic map of seaweed-derived bioactives and their molecular targets to visualize potential synergistic interactions. A cross-phylum analysis is performed to compare the pharmacological activities. Additionally, recent limitations and advances in omics-based approaches for elucidating seaweed-host interactions are discussed.

伤口愈合是一个动态的生物过程，包括止血、炎症、增殖和组织重塑。在慢性伤口中，这些阶段的破坏会导致长期的炎症、氧化应激和微生物感染，给临床带来重大挑战。海洋大型藻类是生物活性化合物的重要来源，包括硫酸多糖（岩藻聚糖、卡拉胶、海藻酸盐）、多酚（褐藻单宁）、色素（岩藻黄素）、脂肪酸和甾醇，它们具有抗氧化、抗炎、抗菌和细胞再生特性，对伤口修复至关重要。这些化合物可调节NF-κB、MAPK、COX-2和Nrf2等关键分子通路，减少氧化损伤，抑制促炎细胞因子，抑制生物膜形成病原体。最近的进展表明，将海藻衍生的生物活性物质掺入水凝胶、纳米复合材料和支架中，可以改善伤口愈合、药物传递以及与传统治疗的协同相互作用。本文综述了藻类生物活性物质的药理机制，探讨了它们与先进生物材料的结合，并强调了提高治疗效果的协同方法。在这篇综述中，我们构建了海藻衍生生物活性及其分子靶点的协同图，以可视化潜在的协同相互作用。进行跨门分析以比较其药理活性。此外，最近的局限性和进展，以组学为基础的方法阐明海藻与宿主的相互作用进行了讨论。

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引用次数: 0

Comparative analysis of isoprostanoid profiles in Chlorella sorokiniana grown under autotrophic and heterotrophic conditions 自养和异养条件下小球藻异前列腺素谱的比较分析

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2025-11-11 DOI: 10.1016/j.algal.2025.104424

Tiago Conde , Diana Lopes , Valérie Gros , Guillaume Reversat , Camille Oger , Jean-Marie Galano , Claire Vigor , Alexandre M.C. Rodrigues , Natacha Coelho , Helena Cardoso , M. Rosário Domingues , Thierry Durand

Oxylipins are bioactive lipid mediators derived from polyunsaturated fatty acids (PUFAs), with roles in oxidative stress responses, immunomodulation, and inflammation. While microalgae are recognized as valuable sources of oxylipins, their profiles remain less studied across different species and cultivation conditions. In this study, we characterized the non-enzymatic oxylipin profile of Chlorella sorokiniana grown under autotrophic and heterotrophic conditions to assess the influence of cultivation strategies on their production. A total of 22 isoprostanoids, mainly Phytoprostanes (PhytoP), Phytofurans (PhytoF), Isoprostanes (IsoP), and Neuroprostanes (NeuroP). Autotrophic cultivation resulted in a higher accumulation of isoprostanoids, particularly the α-linolenic acid (ALA) derivatives, PhytoP and PhytoF species, likely due to oxidative stress induced by fluctuating light and temperature conditions. In contrast, heterotrophic growth, performed under controlled conditions, yielded lower overall oxylipin levels highlighting the presence of 10(R)-10-F_4t-NeuroP which was only present in heterotrophic Chlorella. We observed a correlation between the PUFA composition of Chlorella and its non-enzymatic oxylipin profile. Notably, several oxylipins identified in Chlorella have been associated with anti-inflammatory, immunomodulatory, and neuroprotective properties, emphasizing the potential of this microalga as a source of high-value bioactive oxylipins. This study paves the way to the utilization of Chlorella as a source of bioactive oxylipins, as well as to develop cultivation strategies to enhance the production of these lipid mediators.

氧化脂素是源自多不饱和脂肪酸（PUFAs）的生物活性脂质介质，在氧化应激反应、免疫调节和炎症中发挥作用。虽然微藻被认为是有价值的氧化脂素来源，但它们在不同物种和培养条件下的特征研究仍然较少。在本研究中，我们研究了在自养和异养条件下生长的小球藻的非酶促氧脂质谱，以评估培养策略对其产量的影响。共有22种异前列腺素，主要有植物前列腺素（PhytoP）、植物呋喃（PhytoF）、异前列腺素（IsoP）和神经前列腺素（NeuroP）。自养培养导致异前列腺素积累增加，尤其是α-亚麻酸（ALA）衍生物、PhytoP和PhytoF，这可能是由于光照和温度波动导致的氧化应激所致。相比之下，在受控条件下进行的异养生长产生了较低的总氧脂素水平，突出了仅存在于异养小球藻中的10(R)-10-F4t-NeuroP的存在。我们观察到小球藻的PUFA组成与其非酶促氧脂质谱之间的相关性。值得注意的是，在小球藻中发现的几种氧化脂类具有抗炎、免疫调节和神经保护特性，强调了这种微藻作为高价值生物活性氧化脂类来源的潜力。本研究为利用小球藻作为生物活性氧化脂质的来源，以及制定培养策略以提高这些脂质介质的产量铺平了道路。

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引用次数: 0

Novel algal modular LOV domain proteins expand opto-biotechnological avenues for controlling of eukaryotic riboswitching, translational and proteolytic processes 新型藻类模块化LOV结构域蛋白为控制真核生物的核开关、翻译和蛋白水解过程拓展了光生物技术途径

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2025-11-11 DOI: 10.1016/j.algal.2025.104429

Manisha, Rajani Singh, Suneel Kateriya

Light, Oxygen, or Voltage (LOV) domains mediate blue light-gated signal transduction, regulating diverse opto-biological functions. LOV domain functions either standalone or fused with effector domains, regulating the downstream signalling process. The current repertoire of LOV domain-based tools is limited to a relatively small number of naturally occurring proteins. In this study, we have identified novel algal LOV domains fused with different effector domains as potential light-mediated translational, ribogenetic and proteolytic switches, highlighting their unexplored avenues of opto-biotechnology. LOV-domain fusion with eIF4E suggests its potential as light-controlled translational switch and as an opto-ribogenetic regulator. LOV-SppA would be used as light-gated cellular proteolytic switch. LOV-HDAC could be a potential opto-modulated epigenetic regulator. Additionally, LOV coupled with UFD1, UbiH, mannosyl-oligosaccharide glucosidase, HDAC, SppA1 and biosynthetic gene cluster (BCG) molecular chassis pave the way for opto-biomanufacturing strategies of relevant valuable algal bioactive. Moreover, LOV domain distribution shows gradual evolutionary expansion across diverse algal lineages. Here, we report the discovery of 14 novel algal modular LOV domain-containing proteins across the algal system through comprehensive bioinformatics and systems biology approaches. It offers important insights into the structural and functional diversity of LOV photoreceptors in diverse algae. Hence, these newly identified modular LOV domain-containing proteins expand the platform of opto-biotechnology applications. These findings lay foundation for future research on the mechanistic basis for light-driven (LOV domain-mediated) signalling cascade of RNA, translational and protein homeostasis in algae, and potentiate development of next-generation opto-biotechnological tools for optogenetics and opto-biomanufacturing of valuable bioactive via regulation of BGC in green lineage.

光、氧或电压（LOV）结构域介导蓝光门控信号转导，调节多种光生物学功能。LOV结构域可以独立或与效应结构域融合，调节下游信号传导过程。目前基于LOV结构域的工具仅限于相对少量的天然存在的蛋白质。在这项研究中，我们发现了新的藻类LOV结构域与不同的效应结构域融合，作为潜在的光介导翻译，核糖体和蛋白水解开关，突出了它们未被探索的光生物技术途径。lov结构域与eIF4E的融合表明其作为光控翻译开关和光核素调控因子的潜力。LOV-SppA将被用作光门控细胞蛋白水解开关。LOV-HDAC可能是一种潜在的光调节表观遗传调节剂。此外，LOV与UFD1、UbiH、甘露糖寡糖葡萄糖苷酶、HDAC、SppA1和生物合成基因簇（BCG）分子基偶联，为相关有价值的藻类生物活性物的光生物制造策略铺平了道路。此外，LOV结构域的分布在不同的藻类谱系中表现出渐进的进化扩张。在这里，我们报告了通过综合生物信息学和系统生物学方法在藻类系统中发现的14种新的藻类模块化LOV结构域蛋白。它为了解不同藻类中LOV光感受器的结构和功能多样性提供了重要的见解。因此，这些新发现的模块化LOV结构域蛋白扩展了光生物技术的应用平台。这些发现为进一步研究藻类中光驱动（LOV结构域介导）信号级联、翻译和蛋白质稳态的机制奠定了基础，并为开发下一代光生物技术工具奠定了基础，这些光生物技术工具可通过调控绿色谱系中的BGC来实现光遗传学和光生物制造。

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引用次数: 0

CFD-based investigation of sparger position and aeration rate in inclined flat plate photobioreactors 基于cfd的斜平板光生物反应器中分散器位置和曝气速率的研究

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2025-11-11 DOI: 10.1016/j.algal.2025.104423

Behnam Amanna , Parisa A. Bahri , Navid R. Moheimani

Optimizing photobioreactor (PBR) design is essential for improving the productivity and energy efficiency of microalgal cultivation systems. This study employed Computational Fluid Dynamics (CFD) simulations to assess the hydrodynamic performance of an inclined flat plate PBR for Arthrospira platensis cultivation. The CFD model, validated against experimental data (maximum discrepancy: 8.4 %, R² = 0.81), reliably predicted biomass productivity and internal flow dynamics. Five sparger configurations and four aeration rates were investigated for their effects on radial velocity, turbulence kinetic energy (TKE), and dead zone formation.

The results highlighted the hydrodynamic advantages of the rear-most sparger position (R). At 0.21 vvm (volume of air per volume of culture per minute), position R achieved a radial velocity of 0.125 m·s⁻¹, a TKE of 4.32 × 10⁻³ m²·s⁻², and a dead zone fraction of 18.23 %, closely matching the middle sparger position at 0.23 vvm. Notably, 0.23 vvm represented the highest tolerable aeration rate experimentally, as exceeding this threshold induced shear-related mechanical stress, negatively impacting microalgal cell integrity and reducing productivity. Thus, sparger position R provided equivalent mixing at reduced aeration, lowering energy demand and operational stress.

The inclined geometry enhanced flow uniformity and turbulence, particularly with rearward sparger placement. Integrating dead zone analysis with velocity and TKE metrics, this study offers a validated framework for optimizing sparger design in inclined flat plate PBRs. These findings have significant implications for improving energy efficiency and scalability in laboratory and industrial scale microalgal cultivation systems.

优化光生物反应器（PBR）设计对于提高微藻培养系统的生产效率和能源效率至关重要。本研究采用计算流体力学（CFD）模拟方法，对斜平板式平台人工养殖平台的水动力性能进行了研究。根据实验数据验证了CFD模型（最大差异为8.4%,R2 = 0.81），该模型可靠地预测了生物量生产力和内部流动动力学。研究了5种分散器构型和4种曝气率对径向速度、湍流动能（TKE）和死区形成的影响。结果表明，最后间隔位置(R)具有水动力优势。在0.21 vvm（每体积培养物每分钟的空气体积）下，R位置的径向速度为0.125 m·s−1，TKE为4.32 × 10−3 m2·s−2，死区分数为18.23%，与0.23 vvm的中间分散位置非常接近。值得注意的是，0.23 vvm是实验中最高的可耐受曝气率，因为超过这个阈值会引起剪切相关的机械应力，对微藻细胞的完整性产生负面影响，并降低生产力。因此，分散位置R在低曝气条件下提供了等效的混合，降低了能量需求和操作应力。倾斜的几何形状增强了流动均匀性和湍流性，特别是在后部放置分散器时。该研究将死区分析与速度和TKE指标相结合，为倾斜平板pbr中分散器的优化设计提供了一个经过验证的框架。这些发现对提高实验室和工业规模微藻培养系统的能源效率和可扩展性具有重要意义。

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引用次数: 0

Optimizing aquaculture sustainability: Microalgae-based co-culture systems for aquaculture wastewater treatment and pollution reduction 优化水产养殖的可持续性：用于水产养殖废水处理和减少污染的微藻共培养系统

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2025-11-11 DOI: 10.1016/j.algal.2025.104430

Javaeria Maqsood Gill , Syed Makhdoom Hussain , Shafaqat Ali , Ameer Fawad Zahoor , Abdulrahman Alasmari , Muhammad Munir , Eman Naeem , Muhammad Amjad , Zeeshan Yousaf , Muhammad Faisal

Aquaculture, as a growing sector of global food production, faces critical environmental challenges related to nutrient pollution and wastewater management. To address these issues, microalgae-based co-culture systems have emerged as a promising approach for optimizing aquaculture sustainability. These systems leverage the synergistic interactions between microalgae and other organisms, including bacteria, fungi, cyanobacteria, and invertebrates such as zooplankton and filter feeders, to enhance the efficiency of nutrient removal and promote water quality. Microalgae, through their biological processes, can assimilate excess nitrogen and phosphorus, mitigate harmful pollutants, and generate valuable biomass for various commercial applications. The incorporation of microalgae into aquaculture not only facilitates effective wastewater treatment but also offers economic benefits by producing byproducts that can be reintegrated into the production cycle. This review aims to provide a comprehensive analysis of the potential of microalgae-based co-culture systems in aquaculture wastewater treatment, focusing on their environmental, economic, and commercial advantages. It explores the mechanisms by which microalgae interact with other organisms to improve wastewater quality, examines the feasibility and cost-effectiveness of implementing these systems, and highlights current advancements and future research directions in optimizing aquaculture sustainability through microalgae-based technologies.

水产养殖作为全球粮食生产中一个不断增长的部门，面临着与营养物污染和废水管理有关的严峻环境挑战。为了解决这些问题，以微藻为基础的共培养系统已成为优化水产养殖可持续性的一种有希望的方法。这些系统利用微藻和其他生物（包括细菌、真菌、蓝藻）以及浮游动物和滤食性动物等无脊椎动物之间的协同作用，提高营养物去除的效率，改善水质。微藻通过其生物过程，可以吸收多余的氮和磷，减轻有害污染物，并为各种商业应用产生有价值的生物质。将微藻纳入水产养殖不仅有助于有效地处理废水，而且通过产生可重新纳入生产周期的副产品提供经济效益。本文旨在全面分析基于微藻的共培养系统在水产养殖废水处理中的潜力，重点介绍其环境、经济和商业优势。它探讨了微藻与其他生物相互作用以改善废水质量的机制，检查了实施这些系统的可行性和成本效益，并强调了通过基于微藻的技术优化水产养殖可持续性的当前进展和未来研究方向。

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引用次数: 0

Optimized illuminance operation—A light-driven dilution strategy to improve microalgae biomass productivity 优化光照操作——一种提高微藻生物量生产力的光驱动稀释策略

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2025-11-10 DOI: 10.1016/j.algal.2025.104421

Song Gao , Nathan Beirne , Bruno Klein , Scott Edmundson , Geetanjali Yadav , Ryan Davis , Michael Huesemann

Periodic dilution is a necessary operation to avoid light limitation due to self-shading as the culture grows dense. However, environmental conditions are constantly changing in outdoor cultivation systems, making it difficult to determine the optimal dilution rate. To address this challenge, this study evaluated a dilution approach based on light penetration to optimize illuminance (OptiLum) in the culture. Biomass concentration was controlled via sensor-feedback directed dilution to ensure that light reaching the bottom of the culture was maintained above the compensation intensity. Under replicated outdoor pond conditions, by keeping the entire culture within a net-positive photosynthetic zone, the OptiLum operation improved the biomass productivities of two top-performing strains, Picochlorum celeri and Tetraselmis striata, by 95 % and 86 %, respectively, compared to conventional semi-continuous batch cultivation. The dilution rate varied daily and was dynamically adjusted based on the light status within the culture, which is concurrently influenced by weather, culture density, and growth rate. The techno-economic analysis showed that the OptiLum operation could reduce biomass production cost by as much as 24 % and 33 % for P. celeri and T. striata, respectively, assuming a low-cost dewatering approach with initial gravity settling of biomass can be realized for both strains. However, a more costly two-stage dewatering strategy, comprising only membranes and centrifuges, may be necessary for non-settling strains, such as P. celeri, which would alternatively increase production costs by 23 % for the OptiLum case. The results demonstrated that the proposed OptiLum operation is a promising approach to improve biomass productivity and lower production cost via weather-responsive and self-adjusting dilution.

定期稀释是必要的操作，以避免由于培养物变密而产生的自遮光限制。然而，在室外栽培系统中，环境条件是不断变化的，因此很难确定最佳稀释率。为了解决这一挑战，本研究评估了一种基于光穿透的稀释方法，以优化培养物的照度（OptiLum）。通过传感器反馈定向稀释控制生物量浓度，以确保到达培养底部的光保持在补偿强度以上。在重复的室外池塘条件下，通过将整个培养保持在净正光合区，OptiLum操作使两种表现最好的菌株——细绒皮氯菌（Picochlorum celeri）和条纹Tetraselmis striata的生物量生产力分别比常规半连续分批培养提高了95%和86%。稀释率每天都在变化，并根据培养皿内的光照状况动态调整，光照状况同时受天气、培养皿密度和生长速度的影响。技术经济分析表明，假设两种菌种均能实现初始重力沉降的低成本脱水方法，OptiLum操作可分别使celeri和T. striata的生物质生产成本降低24%和33%。然而，对于不沉淀的菌株，如P. celeri，可能需要更昂贵的两阶段脱水策略，仅包括膜和离心机，这可能会使OptiLum的生产成本增加23%。结果表明，OptiLum操作是一种很有前途的方法，可以通过天气响应和自我调节稀释来提高生物质生产力并降低生产成本。

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引用次数: 0