Algal Research-Biomass Biofuels and Bioproducts最新文献_第6页

Exploring the associations among halogenated compounds in Asparagopsis taxiformis: temporal shifts and bacterial impacts under intensive cultivation 探索taxxiformasopsis中卤化化合物之间的关系：在集约化栽培下的时间变化和细菌影响

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2026-03-01 Epub Date: 2025-12-30 DOI: 10.1016/j.algal.2025.104513

S. Blanco-González , P.R. Brooks , D.A. Heyne , A.H. Campbell , S.F. Cummins , N.A. Paul

Bromoform is the key antimethanogenic bioactive in Asparagopsis species, yet its variation in culture and relationship with other halogenated compounds remain poorly understood. Here, the profile of 9 halogenated compounds in A. taxiformis sporophytes cultured at 0.5 g/L over five weeks were quantified, comparing control cultures to those exposed to gentamicin (10 and 20 mg/L) which temporarily reduced surface bacteria by 81–95 %. Chemical analyses (n = 75) identified three classes of compounds (haloacids, halomethanes and haloacetones) comprising totals of 1–3.3 % dry weight (dw). Haloacids represented half the total; dibromoacetic acid (DBAA, 31.4 %), tribromoacetic acid (TBAA, 10 %) and bromochloroacetic acid (1.3 %). Halomethanes accounted for 39.5 % with bromoform (36.4 %) the single most abundant compound at 0.44–1.38 % dw. DBAA and TBAA concentrations reached 0.47–0.82 % and 0.13–0.38 % dw, respectively. Haloacetones contributed 10.7 % of the total, positively correlating with five other halogenated compounds. However, DBAA and TBAA concentrations correlated more closely to growth, cell morphology and bacterial densities than to other halogenated compounds. Overall, there was a temporal shift in growth rates for both antibiotic treatments – initially suppressing growth by 10–25 % before stabilising with all treatments converging to a specific growth rate of ~8.5 % day⁻¹ by week 5. Extending the cultures for an additional 10 weeks confirmed no long-term impacts of antibiotic treatments on growth, halogenated compounds or the composition of surface bacterial community. This study is the first comprehensive evaluation of halogenated compound loads in cultured Asparagopsis, identifying TBAA as a major component that may contribute to antimethanogenic activity in ruminant feed.

溴仿是天冬酰胺中主要的抗甲烷生物活性物质，但其在培养过程中的变化及其与其他卤化化合物的关系尚不清楚。本研究对0.5 g/L浓度下培养5周的A. taxformis孢子体中9种卤化化合物的分布进行了量化，并将对照培养物与暴露于庆大霉素（10和20 mg/L）的培养物进行了比较，庆大霉素暂时减少了81 - 95%的表面细菌。化学分析（n = 75）确定了三类化合物（卤酸、卤甲烷和卤丙酮），总含量为干重（dw）的1 - 3.3%。卤代酸占总数的一半；二溴乙酸（DBAA, 31.4%）、三溴乙酸（TBAA, 10%）和溴氯乙酸（1.3%）。卤甲烷占39.5%，溴仿占36.4%，含量最高，为0.44 - 1.38%。DBAA和TBAA浓度分别为0.47 ~ 0.82%和0.13 ~ 0.38%。卤代酮贡献了10.7%的总量，与其他五种卤代化合物正相关。然而，与其他卤化化合物相比，DBAA和TBAA浓度与生长、细胞形态和细菌密度的关系更为密切。总的来说，两种抗生素治疗的生长速度都有时间变化——最初抑制生长10 - 25%，然后稳定下来，所有治疗在第5周收敛到约8.5%的特定生长速度。将培养物再延长10周，证实抗生素治疗对生长、卤化化合物或表面细菌群落的组成没有长期影响。本研究首次对培养的天冬酰胺中卤化化合物负荷进行了综合评价，确定了TBAA可能是反刍动物饲料中抗甲烷活性的主要成分。

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引用次数: 0

Symbiotic interaction of Chlorella sp. and Shinella sp. for enhanced dissolved organic matter removal from sterilized mixed wastewater 小球藻和Shinella的共生作用对混合废水中溶解性有机物的去除效果

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2026-03-01 Epub Date: 2025-12-30 DOI: 10.1016/j.algal.2025.104501

Rumeng Lu , Qiaoyun Huang , Yunpu Wang , Xian Cui , Zhigang Yu , Naif Abdullah Al-Dhabi , Xiang Wang , Roger Ruan , Wangwang Tang , Zihan Li , Ting Zhou , Yuhuan Liu , Qi Zhang

Microalgae-bacteria symbiont (ABS) presents promising applications in wastewater treatment. However, the synergistic mechanisms underlying pollutants removal by ABS, particularly for dissolved organic matter (DOM), remain inadequately understood. This study evaluated the performance of a symbiotic system of Chlorella sp. and Shinella sp. in different real wastewater scenarios (WW1: mixture of anaerobic digestion effluent (ADE); WW2: mixture of ADE and Baijiu wastewater) across different hydraulic retention times (HRTs). Results demonstrate that ABS significantly outperformed both monocultures, achieving not only markedly higher biomass production (up to 535 % increase) and pollutant removal, but also exhibiting uniquely enhanced capacity for removing DOM components. Notably, these enhanced-removal DOM constituents accounted for over 20 % of the total differential DOM abundance in both wastewater types. Spectroscopic and UPLC-MS/MS analyses revealed that this enhanced performance was driven by active metabolite exchange between microalgae and bacteria, with amino acids, B vitamins, phytohormones (e.g., indoleacetic acid and gibberellins), and signaling molecules such as jasmonic acid underpinning this synergistic relationship. These interactions collectively regulated microbial growth, metabolism, and oxidative stress, thereby improving DOM removal. The resulting biomass was rich in proteins (60 %) and C16–18 fatty acids (96.34 %), thus representing a promising candidate for valorization. This study elucidates the metabolic and signaling mechanisms underlying the enhanced DOM removal in ABS, offering insights for scalable wastewater bioremediation and resource recovery.

微藻-细菌共生体（ABS）在污水处理中具有广阔的应用前景。然而，ABS去除污染物的协同机制，特别是对溶解有机物（DOM）的去除，仍然没有得到充分的了解。本研究评估了小球藻（Chlorella sp.）和Shinella sp.共生系统在不同实际废水场景中的性能(WW1：厌氧消化出水（ADE）的混合物；二战：ADE和白酒废水的混合物)在不同的水力停留时间（HRTs）。结果表明，ABS显著优于两种单一培养，不仅实现了显著更高的生物质产量（高达535%）和污染物去除，而且还表现出独特的增强的去除DOM成分的能力。值得注意的是，这些增强去除的DOM成分占两种废水中总DOM丰度差异的20%以上。光谱和UPLC-MS/MS分析表明，微藻和细菌之间的代谢物交换是微藻性能增强的主要原因，氨基酸、B族维生素、植物激素（如吲哚乙酸和赤霉素）和茉莉酸等信号分子支撑了这种协同关系。这些相互作用共同调节微生物生长、代谢和氧化应激，从而改善DOM的去除。所得到的生物量富含蛋白质（60%）和C16-18脂肪酸（96.34%），因此代表了有希望的增值候选者。本研究阐明了ABS中增强DOM去除的代谢和信号机制，为大规模废水生物修复和资源回收提供了见解。

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引用次数: 0

Innovative indoor hydroponic co-cultivation: Light-driven optimization of lettuce growth and microalgal Chlorella sp. G049 biomass with nutritional and biodiesel potentials 创新室内水培共栽培：生菜生长和具有营养和生物柴油潜力的微藻小球藻G049生物量的光照优化

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2026-03-01 Epub Date: 2026-01-23 DOI: 10.1016/j.algal.2026.104548

Jeeraporn Pekkoh , Kansinee Nuchtako , Wageeporn Maneechote , Apiwit Kamngoen , Antira Wichaphian , Wasu Pathom-aree , Yupa Chromkaew , Benjamas Cheirsilp , Kuan Shiong Khoo , Shuhao Huo , Piroonporn Srimongkol , Sirasit Srinuanpan

This study investigated the potential of an innovative indoor hydroponic co-cultivation system for lettuce (Lactuca sativa L. var. longifolia) and Chlorella sp. G049 microalga, addressing the growing demand for sustainable food production and renewable bioresources. The primary aim was to optimize the growth and biochemical profiles of both organisms by systematically evaluating various LED light wavelengths. In this integrated system, lettuce and Chlorella sp. G049 were co-cultivated under controlled white, red, green, and blue LED lights for 21 days across three successive cycles (7 days for each cycle). Comprehensive assessments included lettuce growth parameters, microalgal biomass accumulation, total lipid content, and the quality of the extracted lipids. Results distinctly showed that red LED light significantly enhanced lettuce growth, promoting robust development and increased photosynthetic pigment production. Conversely, blue LED light maximized microalgal biomass (0.41–0.57 g/L) and total lipid yield (80.67–98.00 mg/L) across all cultivation cycles. The microalgal lipids produced under blue light exhibited notably improved nutritional quality, characterized by a higher proportion of polyunsaturated fatty acids (PUFA; >53%) and favorable health indices. Furthermore, these lipids largely met international biodiesel standards, demonstrating promising cold flow properties (>1.6 °C) and high energy content (>39 MJ/kg). In conclusion, this integrated system offers a highly sustainable and resource-efficient approach for concurrent food and bioresource production, underscoring the significant role of tailored light quality in optimizing output for a circular bioeconomy model. Based on the findings, blue LED light is recommended as the most effective option for simultaneously enhancing lettuce growth and microalgal lipid production, offering substantial benefits for nutritional, bioenergy, and broader sustainable applications.

本研究探讨了莴苣（Lactuca sativa L. var. longifolia）和小球藻（Chlorella sp. G049）室内水培共生系统的潜力，以满足可持续粮食生产和可再生生物资源日益增长的需求。主要目的是通过系统地评估各种LED光波长来优化这两种生物的生长和生化特征。在该综合系统中，生菜和小球藻G049在白、红、绿、蓝4种LED灯控制下共培养21 d，连续3个周期（每个周期7 d）。综合评价包括生菜生长参数、微藻生物量积累、总脂质含量和提取的脂质质量。结果表明，红色LED光显著促进了生菜的生长，促进了生菜的旺盛发育，增加了光合色素的产量。相反，蓝色LED光在所有培养周期中均使微藻生物量（0.41-0.57 g/L）和总脂质产量（80.67-98.00 mg/L）最大化。在蓝光照射下制备的微藻脂质营养品质显著改善，多不饱和脂肪酸（PUFA; >53%）含量较高，健康指数良好。此外，这些脂质在很大程度上符合国际生物柴油标准，表现出良好的冷流动特性（>1.6°C）和高能量含量（>39 MJ/kg）。综上所述，该综合系统为粮食和生物资源同步生产提供了一种高度可持续和资源高效的方法，强调了定制光质量在优化循环生物经济模式产出中的重要作用。基于这些发现，蓝色LED灯被推荐为同时促进生菜生长和微藻脂质产生的最有效选择，在营养、生物能源和更广泛的可持续应用方面提供了实质性的好处。

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引用次数: 0

Phytoplankton communities enriched with small-sized and low-abundance taxa respond to multi-nutrient synergies 小丰度和低丰度浮游植物群落对多养分协同效应有响应

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2026-03-01 Epub Date: 2026-01-25 DOI: 10.1016/j.algal.2026.104535

Jiaqi Chen , Tao Sun , Yanwei Zhao , Wei Yang , Rui Zhang , Qianzhao Sun

Phytoplankton form the foundation of aquatic ecosystems, though some taxa also pose risks by producing algal toxins. Currently, our understanding of how phytoplankton communities, particularly those dominated by small or low-abundance taxa, respond to environmental change remains limited. In this study, we used environmental DNA (eDNA) metabarcoding and morphological surveys to characterize phytoplankton in a typical shallow lake–wetland system and identify potential toxigenic genera. eDNA metabarcoding detected 114 taxa, including 12 potential toxin producers, exceeding morphological surveys in both overall diversity and the number of toxigenic genera. Morphological analyses indicated nutrient-specific response patterns, suggesting that prokaryotic and eukaryotic phytoplankton are mainly associated with phosphorus and nitrogen, respectively. In contrast, eDNA metabarcoding captured more small-sized and low-abundance taxa, exhibiting multi-nutrient nitrogen–phosphorus synergistic effects. This pattern was also evident among potential toxin-producing genera. These findings reveal the role of heterogeneous environments in sustaining phytoplankton diversity and stability, and highlight the importance of dual nitrogen and phosphorus management to reduce ecological risks associated with toxigenic phytoplankton.

浮游植物是水生生态系统的基础，尽管一些分类群也会产生藻类毒素，从而构成风险。目前，我们对浮游植物群落，特别是那些以小型或低丰度分类群为主的浮游植物群落如何响应环境变化的理解仍然有限。本研究利用环境DNA （environmental DNA, eDNA）元条形码和形态调查对典型浅湖湿地系统中的浮游植物进行了特征分析，并鉴定了潜在的产毒属。eDNA元条形码检测到114个分类群，其中包括12个潜在毒素产生者，在总体多样性和产毒属数量上都超过形态学调查。形态学分析表明，原核和真核浮游植物主要与磷和氮有关。相比之下，eDNA元条形码捕获更多的小尺寸和低丰度分类群，表现出多养分氮磷协同效应。这种模式在潜在的产毒属中也很明显。这些发现揭示了异质环境在维持浮游植物多样性和稳定性中的作用，并强调了氮磷双管理对降低浮游植物产毒素相关生态风险的重要性。

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引用次数: 0

Reprogramming carbon metabolism and enhancing the Kennedy pathway promote docosahexaenoic acid biosynthesis in Schizochytrium sp. HX-308 重编程碳代谢和增强Kennedy通路促进Schizochytrium sp. HX-308的二十二碳六烯酸生物合成

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2026-03-01 Epub Date: 2026-01-30 DOI: 10.1016/j.algal.2026.104557

Chunxiao Yan , Zetong Lu , Shuai Zhang , Zhihao Huang , Zixu Zhang , Wang Ma , Xiaoman Sun , Guang Yang

Docosahexaenoic acid (DHA) is a typical ω-3 polyunsaturated fatty acid, which has been shown to improve vision and promote brain development, showing high application value in nutritional supplements. In this study, genetic engineering was carried out on Schizochytrium sp.to promote DHA and lipid accumulation. Firstly, three rate-limiting enzyme genes of glycolysis pathway were successively overexpressed, the optimal engineered strain PYRK-3-P6FK-5-Hex-4 obtained the accumulation of DHA and lipid reaching 29.8 g/L and 66.7 g/L, respectively. To increase precursor and NADPH supply, the genes ACC1, G6PD, ME1, and ME2 were overexpressed in this study, respectively. Notably, the optimal engineered strain G6PD-5 obtained by overexpression G6PD alone had the highest DHA and lipid titers, which were 30.1 g/L and 68.8 g/L respectively, significantly higher than those of PYRK-3-P6FK-5-Hex-4. Considering the limitations of multi-gene editing technology, the optimal engineered strain G6PD-5-ACC1(P_A5150)-4 was obtained by overexpressing G6PD and ACC1. Additionally, mutants DGAT2A* and DGAT2B* were obtained by mutating the conserved amino acid sequences of DGAT2A and DGAT2B. Among them, overexpression of DGAT2B* has the best promoting effect on DHA biosynthesis. Furthermore, the engineered strain G6PD-5-ACC1(P_A5150)-4-DGAT2B*-6 was obtained by overexpressing DGAT2B*, which had the highest DHA and lipid accumulation, reaching 37.7 g/L and 78.8 g/L, respectively. Finally, the engineered strain was fed batch fermented in a 5 L bioreactor, and the titers of DHA and lipid were 38.0 g/L and 79.9 g/L. This study laid the foundation for the industrial production of DHA and provided guidance for the efficient biosynthesis of other lipid compounds.

二十二碳六烯酸（DHA）是一种典型的ω-3多不饱和脂肪酸，已被证明具有改善视力和促进大脑发育的作用，在营养补充剂中具有很高的应用价值。本研究通过对Schizochytrium sp.进行基因工程改造，促进其DHA和脂质积累。首先，糖酵解途径的3个限速酶基因先后过表达，最优工程菌株PYRK-3-P6FK-5-Hex-4的DHA和脂质积累量分别达到29.8 g/L和66.7 g/L。为了增加前体和NADPH的供应，本研究中分别过量表达了ACC1、G6PD、ME1和ME2基因。值得注意的是，仅过表达G6PD获得的最佳工程菌株G6PD-5的DHA滴度和脂质滴度最高，分别为30.1 g/L和68.8 g/L，显著高于PYRK-3-P6FK-5-Hex-4。考虑到多基因编辑技术的局限性，通过过表达G6PD和ACC1获得最佳工程菌株G6PD-5-ACC1(PA5150)-4。此外，通过突变DGAT2A和DGAT2B的保守氨基酸序列，获得突变体DGAT2A*和DGAT2B*。其中，过表达DGAT2B*对DHA生物合成的促进作用最好。此外，通过过表达DGAT2B*获得了G6PD-5-ACC1(PA5150)-4-DGAT2B*-6工程菌株，其DHA和脂质积累量最高，分别达到37.7 g/L和78.8 g/L。最后，在5 L生物反应器中分批发酵，得到DHA滴度为38.0 g/L，脂质滴度为79.9 g/L。本研究为DHA的工业化生产奠定了基础，并为其他脂类化合物的高效生物合成提供了指导。

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引用次数: 0

13C-depletion and high concentration of carbon sources enhance carbon fixation of Nitzschia closterium 13c耗竭和高浓度碳源增强了Nitzschia closterium固碳能力

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2026-03-01 Epub Date: 2026-02-06 DOI: 10.1016/j.algal.2026.104576

Xiaoshuang Deng , Yu Liu , Guoguang Wang , Shuyuan Wang , Guangzhi Rong , Haixia Wang

With the ongoing expansion of the 21st Century Maritime Silk Road, China's maritime trade volume has increased significantly, resulting in a rapid growth in CO₂ emissions from ships. CO₂ derived from fossil fuel combustion has a lower ¹³C/¹²C ratio compared with the atmospheric CO₂. However, the effects of ¹³C-depleted carbon sources from ship emissions on the carbon fixation of algae and the relevant underlying mechanisms remain elusive. In this study, the influences of ¹³C-depletion and high concentration of carbon sources (LA-HCS) on carbon fixation and fatty acid biosynthesis in Nitzschia closterium (N. closterium) were investigated using stable isotope techniques and transcriptomics. Results showed that LA-HCS increased the biomass and CO₂ fixation rate of N. closterium by 40.48% and 37.5%, respectively. Stable isotope analysis showed that LA-HCS reduced the δ¹³C of dissolved inorganic carbon (DIC), promoting ¹²C enrichment in algae cells. Fatty acid quantification showed that LA-HCS increased total fatty acid content, especially monounsaturated fatty acids. The δ¹³C of fatty acids decreased under LA-HCS conditions. This led to the continuous accumulation of ¹²C in fatty acids, promoting the entry of ¹²C into algae. Transcriptomics revealed that LA-HCS upregulated glycolysis, the tricarboxylic acid cycle (TCA cycle), and fatty acid biosynthesis, which increased energy production in algae, accelerated carbon flow and turnover, triggered carbon flow into lipid storage, thereby enhancing the carbon fixation of N. closterium. Overall, this study provided a scientific reference for understanding the mechanisms underlying algae carbon fixation in response to ship emissions.

随着21世纪海上丝绸之路的不断扩张，中国的海上贸易量大幅增加，导致船舶二氧化碳排放量快速增长。与大气中的二氧化碳相比，化石燃料燃烧产生的二氧化碳具有更低的13C/12C比率。然而，船舶排放的13c -贫碳源对藻类固碳的影响及其相关机制尚不清楚。本研究利用稳定同位素技术和转录组学研究了13c耗竭和高浓度碳源（LA-HCS）对Nitzschia closterium （N. closterium）固碳和脂肪酸生物合成的影响。结果表明，LA-HCS使梭状芽孢杆菌生物量和CO2固定率分别提高40.48%和37.5%。稳定同位素分析表明，LA-HCS降低了溶解无机碳（DIC）的δ13C，促进了藻类细胞中12C的富集。脂肪酸定量分析表明，LA-HCS提高了总脂肪酸含量，尤其是单不饱和脂肪酸含量。脂肪酸δ13C在LA-HCS条件下降低。这导致12C在脂肪酸中不断积累，促进12C进入藻类。转录组学研究表明，LA-HCS上调糖酵解、三羧酸循环（TCA）和脂肪酸的生物合成，增加藻类的能量产生，加速碳流和周转，触发碳流进入脂质储存，从而增强N. closterium的固碳能力。总的来说，本研究为理解藻类对船舶排放的固碳反应机制提供了科学参考。

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引用次数: 0

Algal biogenic synthesis of SiO2–CaO–P2O5 system bioactive glass using Cyclotella meneghiniana and waste fish bone 利用meneghiniah Cyclotella和废鱼骨藻类生物合成SiO2-CaO-P2O5体系生物活性玻璃

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2026-03-01 Epub Date: 2026-02-08 DOI: 10.1016/j.algal.2026.104579

Enobong R. Essien , Victor A. Ajisafe , David A. Faluyi , Ngozi A. Adeleye , Violette N. Atasie , Taofikat Abosede Adesalu , Luqman A. Adams , Matthew Julius

Bioactive glasses containing silica and calcium phosphate are desirable in bone repair due to their ability to induce hydroxyapatite (HA) on their surface, from where a cascade of reactions culminating in bone regeneration occurs. Diatom biosilica derived from algae is a green alternative silica precursor to analytical grade reagents. Its environmentally friendly extraction process makes it suitable for the production of biocompatible silicate-based bioactive glasses. Therefore, this study aimed to prepare a bioactive glass in the system SiO₂–CaO–P₂O₅ biologically using the diatom Cyclotella meneghiniana as the silica source and waste catfish (Siluriformes) bone as the precursor for CaO and P₂O₅. The obtained bioactive glass was characterized for its morphology, elemental composition, mineral phase constituents, and bond properties. A standard in vitro procedure in simulated body fluid (SBF) was used to evaluate the bioactivity of the sample, while a cytocompatibility (mouse fibroblasts) test was performed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT assay). The catfish bone contained 64.535 ± 0.394 CaO and 30.818 ± 0.589 P₂O₅ (in wt%), making it a rich source of CaO-P₂O₅. Analysis showed that the Ca/P ratio in the HA deposited on the glass surface was 1.65 after immersion in SBF for 14 days, which is close to that of natural bone (1.67). Besides the bioactivity displayed, the leachate from the bioactive glass was not toxic but exhibited cell viability above 95%. These performances are for a material serving as a scaffold in bone regeneration. The synthetic route where Cyclotella meneghiniana served as a silica substitute precursor is a promising green method for the production of biocompatible silicate bioactive glasses and related silica-based products.

含有二氧化硅和磷酸钙的生物活性玻璃在骨修复中是理想的，因为它们能够在其表面诱导羟基磷灰石（HA），从那里发生一连串的反应最终导致骨再生。从藻类中提取的硅藻生物二氧化硅是分析级试剂的绿色二氧化硅前体替代品。其环保的提取工艺使其适用于生产生物相容性硅酸盐基生物活性玻璃。因此，本研究旨在以meneghiniana硅藻Cyclotella为硅源，废鲶鱼（Siluriformes）骨为CaO和P2O5前体，生物制备具有生物活性的SiO2-CaO-P2O5体系玻璃。所获得的生物活性玻璃的形态、元素组成、矿物相成分和键性质进行了表征。采用模拟体液（SBF）标准体外程序评估样品的生物活性，同时使用3-（4,5-二甲基噻唑-2-基）-2,5-二苯基溴化四唑（MTT法）进行细胞相容性（小鼠成纤维细胞）试验。鱼骨中CaO含量为64.535±0.394，P2O5含量为30.818±0.589 (wt%)，是CaO-P2O5的丰富来源。分析表明，在SBF中浸泡14天后，沉积在玻璃表面的HA的Ca/P比值为1.65，与天然骨的Ca/P比值（1.67）接近。除显示出生物活性外，生物活性玻璃的渗滤液无毒，细胞活力在95%以上。这些性能是用于骨再生的支架材料。以孟氏环tella meneghiniana为硅代前驱体的合成路线是生产生物相容性硅酸盐生物活性玻璃及相关硅基产品的一种很有前途的绿色方法。

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引用次数: 0

Identification and functional analysis of meiotic Spo11 gene homologs in the red alga Gracilariopsis lemaneiformis (Rhodophyta, Gracilariales) 红藻细叶光藓（Gracilariopsis lemaneiformis） Spo11基因减数分裂同源物的鉴定及功能分析

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2026-03-01 Epub Date: 2026-01-05 DOI: 10.1016/j.algal.2026.104517

Enyuan Liang , Mengchao Liu , Ze Yang, Tingyu Chen, Ruyi Dou, Rongxiu Tian, Xiaonan Zang, Di Xu

The meiosis-specific protein Spo11 is evolved from the ancestral A subunit of archaeal Topo VI. Within Archeaplastida, land plants possess two meiotic Spo11 genes and a eukaryotic TopoVIA gene, but it has long been assumed that green and red algae lack Spo11–1. Recent genome projects in red algae including Chondrus crispus, Gracilariopsis chorda and several unicellular taxes have shown that these species often contain more than two Spo11 paralogs: Spo11–1, Spo11–2, and Spo11–3/TopoVIA. Here we cloned three Spo11 genes from the economically important agarophytic red seaweed Gracilariopsis lemaneiformis and designated them GlSpo11–1, GlSpo11–2 and GlSpo11–3 based on sequence alignments and conserved domain analyses. Yeast sporulation assays demonstrated that only GlSpo11–1 and GlSpo11–2 compensated the sporulation defect of the Spo11-deleted Saccharomyces cerevisiae mutant and their complimentary efficiencies did not differ significantly. Quantitative real-time PCR (qRT-PCR) analysis further showed that both genes were upregulated in mature tetrasporophytes relative to immature tissues. Together, these results indicate that GlSpo11–1 and GlSpo11–2 are meiosis specific and that their encoded proteins likely function equivalently, possibly as a heterodimer. In contrast, only GlSpo11–3 interacted with GlTopoVIB in yeast hybrid assays, confirming that GlSpo11–3 represents the eukaryotic TopoVIA subunit and is not involved in meiosis. To the best of our knowledge, this study provides the first functional characterization of three Spo11 paralogs in red algae and suggests that Spo11–1 and Spo11–2 share equal importance in meiosis, consistent with their roles in land plants.

减数分裂特异性蛋白Spo11是从古细菌TopoVI的祖先A亚基进化而来的。在古plastida中，陆地植物具有两个减数分裂Spo11基因和一个真核TopoVIA基因，但长期以来人们一直认为绿藻和红藻缺乏Spo11 - 1。最近对红藻（包括crispus Chondrus， Gracilariopsis chorda和一些单细胞物种）的基因组计划表明，这些物种通常含有两个以上的Spo11类似物：Spo11 - 1， Spo11 - 2和Spo11 - 3/TopoVIA。本研究从经济上具有重要意义的无叶红藻中克隆了3个Spo11基因，并通过序列测序和保守结构域分析将其命名为GlSpo11-1、GlSpo11-2和GlSpo11-3。酵母产孢实验表明，只有GlSpo11-1和GlSpo11-2弥补了spo11缺失的酿酒酵母突变体的产孢缺陷，它们的互补效率没有显著差异。定量实时PCR （qRT-PCR）分析进一步表明，这两个基因在成熟四孢子植物中相对于未成熟组织表达上调。总之，这些结果表明GlSpo11-1和GlSpo11-2是减数分裂特异性的，它们编码的蛋白质可能具有相同的功能，可能是异源二聚体。相比之下，在酵母杂交实验中，只有GlSpo11-3与GlTopoVIB相互作用，证实GlSpo11-3代表真核TopoVIA亚基，不参与减数分裂。据我们所知，本研究首次提供了红藻中三种Spo11类似物的功能表征，并表明Spo11 - 1和Spo11 - 2在减数分裂中具有同等重要性，与其在陆地植物中的作用一致。

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引用次数: 0

Bioprospecting of a native Chlorella sp.: Culture strategies under contrasting light regimes reveal metabolic trade-offs between primary metabolism and phenolic accumulation 原生小球藻的生物勘探：不同光照条件下的培养策略揭示了初级代谢和酚积累之间的代谢权衡

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2026-03-01 Epub Date: 2026-01-05 DOI: 10.1016/j.algal.2026.104518

M.L. Iriarte , C.H. Armando , T.A. Rearte , J.R. Soberón , D.A. Sampietro , M.A. Sgariglia

Bioprospecting of native microalgae provides novel biotechnological resources; however, cultivation strategies that balance growth and metabolite production remain insufficiently defined. A freshwater Chlorella sp. from a Tucumán reservoir (Argentina) was established as an unialgal culture and identified to genus level by transmission electron microscopy.

We assessed the combined influence of culture medium, spectral quality, and photoperiod on growth kinetics and biochemical composition using a two-part experimental design. Experiment 1 assessed spectral quality (white vs. blue LED) in two media (BBM, F/2G), whereas Experiment 2 evaluated photoperiod (12:12 vs. 16:8 h) in both media using white LED. Growth parameters (μ, Td), pigments (chlorophyll a, chlorophyll b, carotenoids), proteins, lipids, carbohydrates, and phenolic compounds were quantified. Cell disruption protocols were optimized for low-biomass samples, enabling complete biochemical profiling at microscale.

Blue light enhanced growth rate but reduced pigment accumulation, whereas white light favored chlorophylls and carotenoids. Phenolics were stimulated under blue light and BBM medium. Photoperiod modulated biochemical allocation: 12:12 favored pigments, proteins, and carbohydrates, while 16:8 promoted phenolics and lipids, consistent with ROS-linked stress responses. Population and biochemical variables were integrated through Principal Component Analysis (PCA) to identify the variables most strongly differentiating treatments.

Together, the results suggested a two-part conceptual cultivation approach in which an initial photoacclimatory phase could be followed by a stress-inducing regime to enhance bioactive metabolites for food, nutraceutical, and agricultural applications. This is the first evaluation of cultivation strategies for freshwater chlorophytes from northwestern Argentina.

原生微藻的生物勘探提供了新的生物技术资源然而，平衡生长和代谢物产生的培养策略仍然不够明确。阿根廷Tucumán水库淡水小球藻（Chlorella sps .）为单藻培养体，经透射电镜鉴定为属水平。我们采用两部分实验设计评估了培养基、光谱质量和光周期对生长动力学和生化组成的综合影响。实验1评估了两种介质（BBM、F/2G）下的光谱质量（白光LED vs蓝光LED），而实验2评估了两种介质下使用白光LED的光周期（12:12 vs 16:8 h）。测定生长参数（μ, Td）、色素（叶绿素a、叶绿素b、类胡萝卜素）、蛋白质、脂类、碳水化合物和酚类化合物。针对低生物量样品，优化了细胞破坏方案，实现了微尺度下的完整生化分析。蓝光促进了生长速度，但减少了色素积累，而白光有利于叶绿素和类胡萝卜素的生长。在蓝光和BBM介质下刺激酚类物质。光周期调节生物化学分配：12:12有利于色素、蛋白质和碳水化合物，16:8促进酚类物质和脂类，与ros相关的应激反应一致。通过主成分分析（PCA）对种群变量和生化变量进行综合分析，找出对不同处理差异最大的变量。综上所述，研究结果建议采用两部分的概念性培养方法，即在初始光适应阶段之后进行应激诱导，以提高食品、营养和农业应用的生物活性代谢物。这是对阿根廷西北部淡水绿藻栽培策略的首次评价。

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引用次数: 0

Research hotspots and evolutionary trends in microalgae-derived EPA production: A bibliometric review 微藻产生EPA的研究热点和进化趋势：文献计量学综述

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2026-03-01 Epub Date: 2026-01-15 DOI: 10.1016/j.algal.2025.104505

Qingting Weng , Xiaoai Lin , Longzao Luo , Lang Tan , Qin Liu , Shiping Lu , Guosheng Li , Zhou Zhang

In response to the growing demand for sustainable sources of omega-3 fatty acids, this study provides a comprehensive evaluation of academic and patent advances in eicosapentaenoic acid (EPA) production from microalgae. A bibliometric analysis was conducted using VOSviewer on publications retrieved from the Web of Science Core Collection and patent documents from Derwent Innovations Index between 2005 and 2024. Keyword co-occurrence and clustering analyses were employed to identify major research themes and temporal trends. The results reveal a steady increase in annual publications, divided into three distinct phases. Patent analysis shows parallel growth in commercial activity with a 2–3-year lag behind academic research peaks. Research spans diverse disciplines, including marine biology, biotechnology, and microbiology. Articles were published in 200 journals, 24 of which each published more than 10 articles each. The United States and China were identified as the leading countries in both publication output and collaborative influence. Author co-occurrence analysis indicated that Martin J. Kainz was the most prolific contributor. High-frequency keywords such as “microalgae”, “growth”, and “EPA” reflect central research themes, with clusters highlighting biological traits and environmental factors. Current research focuses on strain selection, lipid composition, metabolic engineering, and commercial applications. Microalgae-derived EPA represents a promising sustainable alternative to fish oil, with significant potential in nutrition, aquaculture, and the food industry.

为了响应对omega-3脂肪酸可持续来源日益增长的需求，本研究对微藻生产二十碳五烯酸（EPA）的学术和专利进展进行了全面评估。利用VOSviewer对2005 - 2024年间Web of Science核心馆藏的出版物和Derwent Innovations Index的专利文献进行了文献计量分析。关键词共现和聚类分析用于确定主要研究主题和时间趋势。结果显示，年度出版物稳步增长，分为三个不同的阶段。专利分析显示，商业活动的平行增长比学术研究峰值滞后2 - 3年。研究跨越多个学科，包括海洋生物学、生物技术和微生物学。在200种期刊上发表文章，其中24种期刊发表10篇以上。美国和中国被确定为在出版物产出和合作影响力方面领先的国家。作者共现分析表明，Martin J. Kainz是最多产的贡献者。高频关键词如“微藻”、“生长”和“EPA”反映了中心研究主题，集群突出了生物特性和环境因素。目前的研究重点是菌株选择、脂质组成、代谢工程和商业应用。微藻衍生的EPA代表了一种有前途的可持续的鱼油替代品，在营养、水产养殖和食品工业中具有巨大的潜力。

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引用次数: 0