Algal Research-Biomass Biofuels and Bioproducts最新文献_第6页

Comprehensive lipid extract characterization of Chlorella vulgaris microalgae: A multi-analytical approach

IF 4.6 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2025-01-12 DOI: 10.1016/j.algal.2025.103919

Sheetal Mane, Shivani Desai, Anand Kishore, Anupama Singh

This study aimed to assess lipid extracts of Chlorella vulgaris (CV) using traditional (soxhlet) and innovative extraction methods (microwave-assisted and ultrasound-assisted). The evaluation focused on antioxidant properties, fatty acid profiling, thermogravimetric analysis (TGA), and Fourier transform infrared spectroscopy (FT-IR) of lipid extract and raw biomass. Scanning electron microscopy (SEM) of the lipid-extracted biomass (residual biomass) was also analyzed. Principal component analysis (PCA) was used to develop a correlation between the antioxidant properties of soxhlet lipid extract (SLE), microwave lipid extract (MWLE), ultrasound lipid extract (USLE), and raw biomass. The study revealed that the MWLE showed significantly higher antioxidant properties such as total phenolic content (TPC) 10.71 ± 0.15 mg GAE/g, % DPPH inhibition (37.23 ± 0.16 %), ABTS (9.86 ± 0.02 mg TE/g), and FRAP (19.67 ± 0.04 mg TE/g) whereas SLE showed highest total flavonoid content (TFC) 45.92 ± 2.33 mg QE/g. Extracted lipids also showed a suitable fatty acid profile for biodiesel and food applications. Further TGA results depicted that the residual biomass after lipid extraction could be suitable for biochar production. The FT-IR spectroscopy confirmed the presence of lipids in the extracts, while SEM images demonstrated ultrasonication's superior lipid extraction efficiency over other methods (soxhlet and microwave). The findings suggest that CV biomass and lipid extracts has potential applications in the food, feed, and medicine sectors.

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引用次数: 0

Innovative vs. classical methods for drying heterotrophic Chlorella vulgaris: Impact on the nutritional properties, safety, sustainability and costs

IF 4.6 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2025-01-12 DOI: 10.1016/j.algal.2025.103913

Marie-Christin Baune , Edwin Januschewski , Maresa Bussa , Simon Van De Walle , Imma Gifuni , Alexandre Miguel Cavaco Rodrigues , Maria Helena Cardoso , Geert Van Royen , Andreas Juadjur , Niels Jungbluth , Nino Terjung , Massimo Castellari , Fabio Fanari

The microalgae Chlorella vulgaris is an interesting emerging food source, but to compete with other alternative protein sources, production costs must be reduced. The drying process plays a critical role in production as it ensures product stabilization and quality preservation. However, drying is a cost-intensive process and different drying methods and drying parameters can significantly affect the composition and quality of the final product. This study analyzed the effects of the conventional drying methods freeze drying (FD) and spray drying (SprD) as well as the innovative drying methods solar drying (SolD), pulse combustion drying (PCD) and agitated thin film drying (ATFD) on the nutrient composition, the content of polycyclic aromatic hydrocarbons, the microbial count (total bacterial count, yeasts, molds, E. coli) as well as the environmental impact and production cost. Except for ATFD, showing changes in the carbohydrate and fiber content, the effects of drying methods on nutrient composition were minor. SprD was identified as having the greatest environmental impact and being the most cost-intensive, whereas SolD performed best, with only a slight reduction in vitamin B6 and some mineral elements compared to FD. PCD performed second best but needs improvement in terms of drying yield to reduce production costs.

{"title":"Innovative vs. classical methods for drying heterotrophic Chlorella vulgaris: Impact on the nutritional properties, safety, sustainability and costs","authors":"Marie-Christin Baune , Edwin Januschewski , Maresa Bussa , Simon Van De Walle , Imma Gifuni , Alexandre Miguel Cavaco Rodrigues , Maria Helena Cardoso , Geert Van Royen , Andreas Juadjur , Niels Jungbluth , Nino Terjung , Massimo Castellari , Fabio Fanari","doi":"10.1016/j.algal.2025.103913","DOIUrl":"10.1016/j.algal.2025.103913","url":null,"abstract":"<div><div>The microalgae <em>Chlorella vulgaris</em> is an interesting emerging food source, but to compete with other alternative protein sources, production costs must be reduced. The drying process plays a critical role in production as it ensures product stabilization and quality preservation. However, drying is a cost-intensive process and different drying methods and drying parameters can significantly affect the composition and quality of the final product. This study analyzed the effects of the conventional drying methods freeze drying (FD) and spray drying (SprD) as well as the innovative drying methods solar drying (SolD), pulse combustion drying (PCD) and agitated thin film drying (ATFD) on the nutrient composition, the content of polycyclic aromatic hydrocarbons, the microbial count (total bacterial count, yeasts, molds, <em>E. coli</em>) as well as the environmental impact and production cost. Except for ATFD, showing changes in the carbohydrate and fiber content, the effects of drying methods on nutrient composition were minor. SprD was identified as having the greatest environmental impact and being the most cost-intensive, whereas SolD performed best, with only a slight reduction in vitamin B6 and some mineral elements compared to FD. PCD performed second best but needs improvement in terms of drying yield to reduce production costs.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"86 ","pages":"Article 103913"},"PeriodicalIF":4.6,"publicationDate":"2025-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143144513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Specific light-regime adaptations, terpenoid profiles and engineering potential in ecologically diverse Phaeodactylum tricornutum strains

IF 4.6 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2025-01-12 DOI: 10.1016/j.algal.2025.103920

Luca Morelli , Payal Patwari , Florian Pruckner , Maxime Bastide , Michele Fabris

Microalgae, and among them, the diatom Phaeodactylum tricornutum stand out with their remarkable versatility and metabolic engineering potential. Diatoms exhibit substantial variability in metabolism, photosynthetic physiology and environmental adaptation, even across the same species. These factors can affect the design and outcome of metabolic engineering strategies. In this study, we profiled the diversity of biotechnologically relevant traits of three P. tricornutum strains (Pt1, Pt6, and Pt9) under different light regimes to identify the most suitable chassis to be employed as bio-factory to produce high-value terpenoids. We conducted detailed assessments of these strains, using pulse amplitude modulated (PAM) fluorometry to measure photosynthetic efficiency and we analyzed the composition of pigments and triterpenoids, as main terpenoid metabolic sinks. Parameters such as the maximum quantum yield of PSII (Fv/Fm), the efficiency of excitation energy capture (Fv’/Fm'), and OJIP kinetics were used to estimate photosynthetic performance in different light regimes. Additionally, we evaluated their transformation efficiency and their capacity to produce heterologous monoterpenoids, using geraniol as a model product. Our findings revealed that Pt1, widely used in laboratories, exhibits robust growth and photosynthetic performance under standard laboratory conditions. Pt6, adapted to intertidal environments, shows unique resilience in fluctuating conditions, while Pt9, with its high-temperature tolerance, excels under continuous high irradiance. Additionally, this variability across strains and light conditions influenced the metabolic output of each strain. We concluded that understanding the physiological responses of different P. tricornutum strains to light is crucial for optimizing their use in metabolic engineering. The insights gained from this research will facilitate the strategic selection and exploitation of these strains in algae biotechnology, enhancing the production of commercially valuable compounds such as high-value terpenoids and derivatives. This comprehensive characterization of strains under varying light conditions offers a pathway to more efficient and targeted metabolic engineering applications.

{"title":"Specific light-regime adaptations, terpenoid profiles and engineering potential in ecologically diverse Phaeodactylum tricornutum strains","authors":"Luca Morelli , Payal Patwari , Florian Pruckner , Maxime Bastide , Michele Fabris","doi":"10.1016/j.algal.2025.103920","DOIUrl":"10.1016/j.algal.2025.103920","url":null,"abstract":"<div><div>Microalgae, and among them, the diatom <em>Phaeodactylum tricornutum</em> stand out with their remarkable versatility and metabolic engineering potential. Diatoms exhibit substantial variability in metabolism, photosynthetic physiology and environmental adaptation, even across the same species. These factors can affect the design and outcome of metabolic engineering strategies. In this study, we profiled the diversity of biotechnologically relevant traits of three <em>P. tricornutum</em> strains (Pt1, Pt6, and Pt9) under different light regimes to identify the most suitable chassis to be employed as bio-factory to produce high-value terpenoids. We conducted detailed assessments of these strains, using pulse amplitude modulated (PAM) fluorometry to measure photosynthetic efficiency and we analyzed the composition of pigments and triterpenoids, as main terpenoid metabolic sinks. Parameters such as the maximum quantum yield of PSII (Fv/Fm), the efficiency of excitation energy capture (Fv’/Fm'), and OJIP kinetics were used to estimate photosynthetic performance in different light regimes. Additionally, we evaluated their transformation efficiency and their capacity to produce heterologous monoterpenoids, using geraniol as a model product. Our findings revealed that Pt1, widely used in laboratories, exhibits robust growth and photosynthetic performance under standard laboratory conditions. Pt6, adapted to intertidal environments, shows unique resilience in fluctuating conditions, while Pt9, with its high-temperature tolerance, excels under continuous high irradiance. Additionally, this variability across strains and light conditions influenced the metabolic output of each strain. We concluded that understanding the physiological responses of different <em>P. tricornutum</em> strains to light is crucial for optimizing their use in metabolic engineering. The insights gained from this research will facilitate the strategic selection and exploitation of these strains in algae biotechnology, enhancing the production of commercially valuable compounds such as high-value terpenoids and derivatives. This comprehensive characterization of strains under varying light conditions offers a pathway to more efficient and targeted metabolic engineering applications.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"86 ","pages":"Article 103920"},"PeriodicalIF":4.6,"publicationDate":"2025-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143144493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of unconventional pretreatments on the morphology and biochemical methane potential of Sargassum spp.

IF 4.6 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2025-01-12 DOI: 10.1016/j.algal.2025.103915

Rosy Paletta , Sebastiano Candamano , Mauro Daniel Luigi Bruno , Giovanni Desiderio , Yessica A. Castro

Sargassum spp. blooms disrupt Caribbean coastal ecology, economy, and community health. Methane production is a promising solution for valorizing this macroalgae and cutting macroalgae management costs. However, pretreatment is essential to address low efficiency due to the presence of lignocellulose and calcite in the biomass morphology. In this work we evaluate the effects of non-chemically driven pretreatments (i.e. microwave, sonication, and water wash at 40 °C) on the morphology and biochemical methane potential (BMP) of the Sargassum spp. biomass. The morphological properties of the biomass were determined using X-ray diffractometry (XRD), FT-IR, SEM. The BMP was evaluated using an automated potential test system (AMPTS® II). The yield of the untreated biomass was equal to 72.25 NmL/g VS. The microwave pre-treatment induced a 26.06 % reduction in yield, primarily due to the formation of recalcitrant components. Sonication pretreatment led to a 5.28 % increase in yield, attributed to the cellular-level alterations prompted by cavitation phenomena. In contrast, the water wash pretreatment demonstrated a significant yield enhancement of 20.76 % compared to untreated biomass. However, all the investigated scenarios are not able to generate a positive income by solely selling the obtained electricity.

{"title":"Effect of unconventional pretreatments on the morphology and biochemical methane potential of Sargassum spp.","authors":"Rosy Paletta , Sebastiano Candamano , Mauro Daniel Luigi Bruno , Giovanni Desiderio , Yessica A. Castro","doi":"10.1016/j.algal.2025.103915","DOIUrl":"10.1016/j.algal.2025.103915","url":null,"abstract":"<div><div><em>Sargassum</em> spp. blooms disrupt Caribbean coastal ecology, economy, and community health. Methane production is a promising solution for valorizing this macroalgae and cutting macroalgae management costs. However, pretreatment is essential to address low efficiency due to the presence of lignocellulose and calcite in the biomass morphology. In this work we evaluate the effects of non-chemically driven pretreatments (i.e. microwave, sonication, and water wash at 40 °C) on the morphology and biochemical methane potential (BMP) of the <em>Sargassum</em> spp. biomass. The morphological properties of the biomass were determined using X-ray diffractometry (XRD), FT-IR, SEM. The BMP was evaluated using an automated potential test system (AMPTS® II). The yield of the untreated biomass was equal to 72.25 NmL/g VS. The microwave pre-treatment induced a 26.06 % reduction in yield, primarily due to the formation of recalcitrant components. Sonication pretreatment led to a 5.28 % increase in yield, attributed to the cellular-level alterations prompted by cavitation phenomena. In contrast, the water wash pretreatment demonstrated a significant yield enhancement of 20.76 % compared to untreated biomass. However, all the investigated scenarios are not able to generate a positive income by solely selling the obtained electricity.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"86 ","pages":"Article 103915"},"PeriodicalIF":4.6,"publicationDate":"2025-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143144516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integration of dairy manure wastewater treatment and biofuel production by microalgae-bacteria consortia in a biofilm reactor

IF 4.6 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2025-01-11 DOI: 10.1016/j.algal.2025.103912

Ying Chu , Xiaolin Jiao , Dan Li , Jiawen Yu , Changhai Wang , Meilin He

Microalgae can efficiently remove nutrients and pollutants from wastewater while converting these substances into reusable biomass. This study explores the potential of microalgae for dairy manure wastewater (DMW) treatment via optimizing process conditions, and employing an algal-bacterial biofilm for continuous wastewater treatment coupling with bioproducts production. A pollutant-tolerant microalga Desmodesmus sp. and a bacterium Bacillus megaterium (Ds-Bm), when co-cultured in suspension, demonstrated efficacy in removing COD and ammonia nitrogen of 50 % raw DMW wastewater under an adjusted nitrogen: phosphorous ratio of 16:1. The total lipid content increased by 45.9 % compared to pure algae culture. A continuous algae-bacterial biofilm photobioreactor was then constructed for raw DMW wastewater treatment without dilution, achieving a COD removal efficiency of 85–92.2 % and meeting the emission standards for livestock wastewater. The ammonium nitrogen removal load reached 4.6 g N/m³/d, with a maximum removal efficiency of 46.5 %. Following a 21-day operation, algae biomass notably increased (the yield and productivity were 122.5 g/m² and 3.7 g/m²/d), with a 15.8 % lipid content. Cultivation in DMW led to a significant reduction in the C18:3 polyunsaturated fatty acid content (<12 %), ensuring compliance with biodiesel feedstock standards. Hence, this Ds-Bm biofilm photobioreactor offers promise for effective wastewater treatment and cost-efficient biodiesel production.

{"title":"Integration of dairy manure wastewater treatment and biofuel production by microalgae-bacteria consortia in a biofilm reactor","authors":"Ying Chu , Xiaolin Jiao , Dan Li , Jiawen Yu , Changhai Wang , Meilin He","doi":"10.1016/j.algal.2025.103912","DOIUrl":"10.1016/j.algal.2025.103912","url":null,"abstract":"<div><div>Microalgae can efficiently remove nutrients and pollutants from wastewater while converting these substances into reusable biomass. This study explores the potential of microalgae for dairy manure wastewater (DMW) treatment via optimizing process conditions, and employing an algal-bacterial biofilm for continuous wastewater treatment coupling with bioproducts production. A pollutant-tolerant microalga <em>Desmodesmus</em> sp. and a bacterium <em>Bacillus megaterium</em> (<em>Ds-Bm</em>), when co-cultured in suspension, demonstrated efficacy in removing COD and ammonia nitrogen of 50 % raw DMW wastewater under an adjusted nitrogen: phosphorous ratio of 16:1. The total lipid content increased by 45.9 % compared to pure algae culture. A continuous algae-bacterial biofilm photobioreactor was then constructed for raw DMW wastewater treatment without dilution, achieving a COD removal efficiency of 85–92.2 % and meeting the emission standards for livestock wastewater. The ammonium nitrogen removal load reached 4.6 g N/m<sup>3</sup>/d, with a maximum removal efficiency of 46.5 %. Following a 21-day operation, algae biomass notably increased (the yield and productivity were 122.5 g/m<sup>2</sup> and 3.7 g/m<sup>2</sup>/d), with a 15.8 % lipid content. Cultivation in DMW led to a significant reduction in the C18:3 polyunsaturated fatty acid content (<12 %), ensuring compliance with biodiesel feedstock standards. Hence, this <em>Ds-Bm</em> biofilm photobioreactor offers promise for effective wastewater treatment and cost-efficient biodiesel production.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"86 ","pages":"Article 103912"},"PeriodicalIF":4.6,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143144517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Machine learning-assisted synthetic biology of cyanobacteria and microalgae

IF 4.6 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2025-01-11 DOI: 10.1016/j.algal.2025.103911

Weijia Jin , Fangzhong Wang , Lei Chen , Weiwen Zhang

Cyanobacteria and microalgae have recently been utilized as autotrophic chassis for microbial cell factories capable of converting light and CO₂ directly into high-value products. However, compared to other heterotrophic model chassis, they face many challenges such as limited genetic manipulation tools, poorly understood metabolic regulatory networks, and low production efficiency. Machine learning, a data-driven pattern recognition strategy, leverages statistical associations in datasets for classification and generation, proving to be a valuable tool for synthetic biology. Synthetic biology requires the extraction of fundamental component information from complex biological data to create novel applications through the reassembly of biological components. This process aligns well with the capabilities of machine learning. In this review, we briefly introduce the recent progress on how machine learning has assisted in genome reannotation, contributed to the development of genetic manipulation tools, elucidated metabolic networks, and optimized bioprocesses in cyanobacterial and microalgal studies within the context of synthetic biology. Additionally, we analyze the current challenges facing this field and provide perspectives on further research directions for applying machine learning to synthetic biology in cyanobacteria and microalgae.

{"title":"Machine learning-assisted synthetic biology of cyanobacteria and microalgae","authors":"Weijia Jin , Fangzhong Wang , Lei Chen , Weiwen Zhang","doi":"10.1016/j.algal.2025.103911","DOIUrl":"10.1016/j.algal.2025.103911","url":null,"abstract":"<div><div>Cyanobacteria and microalgae have recently been utilized as autotrophic chassis for microbial cell factories capable of converting light and CO₂ directly into high-value products. However, compared to other heterotrophic model chassis, they face many challenges such as limited genetic manipulation tools, poorly understood metabolic regulatory networks, and low production efficiency. Machine learning, a data-driven pattern recognition strategy, leverages statistical associations in datasets for classification and generation, proving to be a valuable tool for synthetic biology. Synthetic biology requires the extraction of fundamental component information from complex biological data to create novel applications through the reassembly of biological components. This process aligns well with the capabilities of machine learning. In this review, we briefly introduce the recent progress on how machine learning has assisted in genome reannotation, contributed to the development of genetic manipulation tools, elucidated metabolic networks, and optimized bioprocesses in cyanobacterial and microalgal studies within the context of synthetic biology. Additionally, we analyze the current challenges facing this field and provide perspectives on further research directions for applying machine learning to synthetic biology in cyanobacteria and microalgae.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"86 ","pages":"Article 103911"},"PeriodicalIF":4.6,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143144518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pilot system for engineering sustainable aquatic food webs: Utilizing cyanobacteria for continuous secretion of ω-polyunsaturated fatty acids

IF 4.6 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2025-01-11 DOI: 10.1016/j.algal.2025.103910

Maximilian K. Lübben , Steffen Heinz , Sabine Gießler , Martin Lehmann , Laura Kleinknecht , Maria Stockenreiter , Matthias Ostermeier , Herwig Stibor , Jörg Nickelsen

This study introduces a pilot system that uses genetically engineered and marker-less strains of Synechocystis sp. PCC 6803 for enhanced secretion of ω-polyunsaturated fatty acids (ω-PUFAs), specifically intended for aquaculture applications. By inactivating the native acyl-acyl carrier protein synthetase (Aas) and expressing the heterologous Fatty Acid Export 1 (FAX1) gene from Arabidopsis thaliana, transgenic cyanobacterial lines were obtained that continuously secrete PUFAs in the C₁₆ to C₁₈ range. Cellular integrity was maintained in these lines, as evidenced by the monitoring of growth rates and photosynthetic parameters. In a controlled aquaculture set-up with the planktonic crustacean Daphnia magna, using dialysis tubing for spatial separation, the engineered strains significantly enhanced the survival, the proportion of egg-bearing females, and the reproductive output of D. magna. These results underline the potential of genetically engineered algae to act as a sustainable feedstock in aquaculture, enhancing both nutritional quality and environmental safety.

{"title":"Pilot system for engineering sustainable aquatic food webs: Utilizing cyanobacteria for continuous secretion of ω-polyunsaturated fatty acids","authors":"Maximilian K. Lübben , Steffen Heinz , Sabine Gießler , Martin Lehmann , Laura Kleinknecht , Maria Stockenreiter , Matthias Ostermeier , Herwig Stibor , Jörg Nickelsen","doi":"10.1016/j.algal.2025.103910","DOIUrl":"10.1016/j.algal.2025.103910","url":null,"abstract":"<div><div>This study introduces a pilot system that uses genetically engineered and marker-less strains of <em>Synechocystis</em> sp. PCC 6803 for enhanced secretion of ω-polyunsaturated fatty acids (ω-PUFAs), specifically intended for aquaculture applications. By inactivating the native acyl-acyl carrier protein synthetase (Aas) and expressing the heterologous Fatty Acid Export 1 (FAX1) gene from <em>Arabidopsis thaliana</em>, transgenic cyanobacterial lines were obtained that continuously secrete PUFAs in the C<sub>16</sub> to C<sub>18</sub> range. Cellular integrity was maintained in these lines, as evidenced by the monitoring of growth rates and photosynthetic parameters. In a controlled aquaculture set-up with the planktonic crustacean <em>Daphnia magna</em>, using dialysis tubing for spatial separation, the engineered strains significantly enhanced the survival, the proportion of egg-bearing females, and the reproductive output of <em>D. magna</em>. These results underline the potential of genetically engineered algae to act as a sustainable feedstock in aquaculture, enhancing both nutritional quality and environmental safety.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"86 ","pages":"Article 103910"},"PeriodicalIF":4.6,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143145025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of subcritical water–mediated liquefaction on compositions and bioactivities of sea lettuce (Ulva prolifera) extracts

IF 4.6 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2025-01-11 DOI: 10.1016/j.algal.2025.103914

Jong Won Lee , SangYoon Lee , Mi-Yeon Lee , Geun-Pyo Hong

Sea lettuce (Ulva prolifera) has become a coastal pollutant despite its benefits for human health. This study investigated the effect of subcritical water (SW) liquefaction on the changes in the composition and physiological activities of sea lettuce. Proteins and polysaccharides from sea lettuce were hydrothermally hydrolyzed with SW at 200–250 °C, and these hydrolysates underwent Maillard reaction, yielding a maximum amount of melanoidin at 250 °C. The SW at 250 °C was effective for the extraction of phenolic compounds from sea lettuce. 2,2′-Azino-bis-(3-ethylbenzothiazoline-6-sulfonic) acid (ABTS) radical inhibition activity and angiotensin-converting enzyme (ACE) inhibition activity indicated that the thermochemical changes induced by SW enabled the conversion of sea lettuce into strong antioxidative and antihypertensive biomaterials. Sea lettuce subjected to a temperature of 150 °C exhibited the best prebiotic activity, whereas lettuce liquefied at 300 °C showed strong antimicrobial activity. The taste profiles of sea lettuce indicated that the simple taste attribute of the control extract was transformed into a complex taste attribute through SW. In particular, sea lettuce subjected to 250 °C had the highest sweetness and umami. Consequently, this study demonstrated that sea lettuce liquefied by SW could be used as a biomaterial and a taste enhancer in food systems, and the SW had notable potential for the upcycling utilization of sea lettuce in the functional food industry.

{"title":"Effect of subcritical water–mediated liquefaction on compositions and bioactivities of sea lettuce (Ulva prolifera) extracts","authors":"Jong Won Lee , SangYoon Lee , Mi-Yeon Lee , Geun-Pyo Hong","doi":"10.1016/j.algal.2025.103914","DOIUrl":"10.1016/j.algal.2025.103914","url":null,"abstract":"<div><div>Sea lettuce (<em>Ulva prolifera</em>) has become a coastal pollutant despite its benefits for human health. This study investigated the effect of subcritical water (SW) liquefaction on the changes in the composition and physiological activities of sea lettuce. Proteins and polysaccharides from sea lettuce were hydrothermally hydrolyzed with SW at 200–250 °C, and these hydrolysates underwent Maillard reaction, yielding a maximum amount of melanoidin at 250 °C. The SW at 250 °C was effective for the extraction of phenolic compounds from sea lettuce. 2,2′-Azino-bis-(3-ethylbenzothiazoline-6-sulfonic) acid (ABTS) radical inhibition activity and angiotensin-converting enzyme (ACE) inhibition activity indicated that the thermochemical changes induced by SW enabled the conversion of sea lettuce into strong antioxidative and antihypertensive biomaterials. Sea lettuce subjected to a temperature of 150 °C exhibited the best prebiotic activity, whereas lettuce liquefied at 300 °C showed strong antimicrobial activity. The taste profiles of sea lettuce indicated that the simple taste attribute of the control extract was transformed into a complex taste attribute through SW. In particular, sea lettuce subjected to 250 °C had the highest sweetness and umami. Consequently, this study demonstrated that sea lettuce liquefied by SW could be used as a biomaterial and a taste enhancer in food systems, and the SW had notable potential for the upcycling utilization of sea lettuce in the functional food industry.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"86 ","pages":"Article 103914"},"PeriodicalIF":4.6,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143144500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lipid and lutein recovery from desertic microalgae in saline wastewater combined to abiotic stressors for sustainable antioxidant and biodiesel production

IF 4.6 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2025-01-10 DOI: 10.1016/j.algal.2025.103903

M.N. Keddar , A. Ballesteros-Gómez , J.A. Siles , S. Rubio , M.A. Martín

Freshwater salinisation is a serious global issue affecting natural resources availability and food production. Salinity-based cultivation systems are considered promising approaches to generate valuable microalgal biomass for energy and food purposes, even though salt stress can compromise growth efficiency and bioproducts productivity markedly. Herein, a single-stage cultivation of two native microalgae in saline wastewater combined to abiotic stress factors was studied. The physicochemical responses of each microalgae including biomass growth, biochemical composition and antioxidant activities were evaluated. The results showed that all the combinations enhanced microalgal growth performance efficiently, with lipids and antioxidant productivities increasing in a simultaneous manner. The biomass contents and productivities were found to vary within the range of 0.58–2.02 g_dw L ^{̶ 1} and 40.50–189.00 mg_dw L ^{̶ 1} d ^{̶ 1}, respectively. The highest lipid accumulation was obtained in the culture of Selenastrum sp. KCC3, under the synergistic effect of 11 g salt L ^{̶ 1} and nitrogen deprivation (36.59 %, dw), as well as in the mixotrophic cultures of Chlorella sorokiniana KCC4 (35.83 %, dw). The lipid productivity varied between 12.50 and 65.66 mg_dw L ^{̶ 1} d ^{̶ 1}, while production of lutein was maximal at 2.04 mg g_dw ^{̶ 1} in Selenastrum sp. and achieved an optimal value of 1.56 mg g_dw ^{̶ 1} under moderate light stress culture of Chlorella sorokiniana. Abiotic factors were also effective in increasing the antioxidant levels and activities of algal extracts, whereas the composition of fatty acid for biodiesel production was also influenced under salt stress. The whole findings provide a promising salinity stress-induced strategy for efficient biorefining of desertic microalgae.

{"title":"Lipid and lutein recovery from desertic microalgae in saline wastewater combined to abiotic stressors for sustainable antioxidant and biodiesel production","authors":"M.N. Keddar , A. Ballesteros-Gómez , J.A. Siles , S. Rubio , M.A. Martín","doi":"10.1016/j.algal.2025.103903","DOIUrl":"10.1016/j.algal.2025.103903","url":null,"abstract":"<div><div>Freshwater salinisation is a serious global issue affecting natural resources availability and food production. Salinity-based cultivation systems are considered promising approaches to generate valuable microalgal biomass for energy and food purposes, even though salt stress can compromise growth efficiency and bioproducts productivity markedly. Herein, a single-stage cultivation of two native microalgae in saline wastewater combined to abiotic stress factors was studied. The physicochemical responses of each microalgae including biomass growth, biochemical composition and antioxidant activities were evaluated. The results showed that all the combinations enhanced microalgal growth performance efficiently, with lipids and antioxidant productivities increasing in a simultaneous manner. The biomass contents and productivities were found to vary within the range of 0.58–2.02 g<sub>dw</sub> L <sup>̶ 1</sup> and 40.50–189.00 mg<sub>dw</sub> L <sup>̶ 1</sup> d <sup>̶ 1</sup>, respectively. The highest lipid accumulation was obtained in the culture of <em>Selenastrum</em> sp. KCC3, under the synergistic effect of 11 g salt L <sup>̶ 1</sup> and nitrogen deprivation (36.59 %, dw), as well as in the mixotrophic cultures of <em>Chlorella sorokiniana</em> KCC4 (35.83 %, dw). The lipid productivity varied between 12.50 and 65.66 mg<sub>dw</sub> L <sup>̶ 1</sup> d <sup>̶ 1</sup>, while production of lutein was maximal at 2.04 mg g<sub>dw</sub> <sup>̶ 1</sup> in <em>Selenastrum</em> sp. and achieved an optimal value of 1.56 mg g<sub>dw</sub> <sup>̶ 1</sup> under moderate light stress culture of <em>Chlorella sorokiniana</em>. Abiotic factors were also effective in increasing the antioxidant levels and activities of algal extracts, whereas the composition of fatty acid for biodiesel production was also influenced under salt stress. The whole findings provide a promising salinity stress-induced strategy for efficient biorefining of desertic microalgae<em>.</em></div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"86 ","pages":"Article 103903"},"PeriodicalIF":4.6,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143144515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effectiveness of four algal technologies in removing nutrients and antibiotics from biogas slurry induced by different concentrations of diethylaminoethyl hexanoate

IF 4.6 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2025-01-10 DOI: 10.1016/j.algal.2025.103906

Wenbo Chai , Qiaoli Wang , Chunzhi Zhao , Zhengfang Wang , Ping Yao , Bing Zhao , Bei Lu , Yongjun Zhao , Xuehong Yuan

This study investigates the effects of different concentrations of exogenously added Diethylaminoethyl Hexanoate (DA-6) on the biomass, photosynthesis, and pollutant removal efficiency of four microalgal treatment systems (Chlorella vulgaris monoculture, Chlorella vulgaris+S395-2+ Pleurotus ostreatus, Chlorella vulgaris+ S395-2+ Ganoderma lucidum, Chlorella vulgaris +S395-2+ Clonostachys rosea) in biogas slurry containing antibiotics. The results indicate that Chlorella vulgaris exhibited optimal growth at a DA-6 concentration of 10⁻⁷ M, with the Chlorella vulgaris + S395-2 + Clonostachys rosea system showing superior performance in all measured parameters compared to other systems. Under optimal DA-6 conditions (10⁻⁷ M), the average removal rates of COD, TN, and TP in the biogas slurry by the Chlorella vulgaris + S395-2 + Clonostachys rosea system were 89.42 ± 7.13 %, 87.71 ± 6.74 %, and 89.85 ± 7.14 %, respectively. The average removal rates of tetracycline hydrochloride (TC), ciprofloxacin hydrochloride (CPFX), and sulfamethoxypyridazine (SMM) were 99.53 ± 0.19 %, 88.26 ± 6.17 %, and 86.31 ± 6.78 %, respectively, with the system demonstrating a more effective reduction of TC antibiotics compared to CPFX and SMM antibiotics.

{"title":"The effectiveness of four algal technologies in removing nutrients and antibiotics from biogas slurry induced by different concentrations of diethylaminoethyl hexanoate","authors":"Wenbo Chai , Qiaoli Wang , Chunzhi Zhao , Zhengfang Wang , Ping Yao , Bing Zhao , Bei Lu , Yongjun Zhao , Xuehong Yuan","doi":"10.1016/j.algal.2025.103906","DOIUrl":"10.1016/j.algal.2025.103906","url":null,"abstract":"<div><div>This study investigates the effects of different concentrations of exogenously added Diethylaminoethyl Hexanoate (DA-6) on the biomass, photosynthesis, and pollutant removal efficiency of four microalgal treatment systems (<em>Chlorella vulgaris</em> monoculture, <em>Chlorella vulgaris+S395-2+ Pleurotus ostreatus</em>, <em>Chlorella vulgaris+ S395-2+ Ganoderma lucidum</em>, <em>Chlorella vulgaris</em> +<em>S395-2</em>+ <em>Clonostachys rosea</em>) in biogas slurry containing antibiotics. The results indicate that <em>Chlorella vulgaris</em> exhibited optimal growth at a DA-6 concentration of 10<sup>−7</sup> M, with the <em>Chlorella vulgaris</em> + <em>S395-2</em> + <em>Clonostachys rosea</em> system showing superior performance in all measured parameters compared to other systems. Under optimal DA-6 conditions (10<sup>−7</sup> M), the average removal rates of COD, TN, and TP in the biogas slurry by the <em>Chlorella vulgaris</em> + <em>S395-2</em> + <em>Clonostachys rosea</em> system were 89.42 ± 7.13 %, 87.71 ± 6.74 %, and 89.85 ± 7.14 %, respectively. The average removal rates of tetracycline hydrochloride (TC), ciprofloxacin hydrochloride (CPFX), and sulfamethoxypyridazine (SMM) were 99.53 ± 0.19 %, 88.26 ± 6.17 %, and 86.31 ± 6.78 %, respectively, with the system demonstrating a more effective reduction of TC antibiotics compared to CPFX and SMM antibiotics.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"86 ","pages":"Article 103906"},"PeriodicalIF":4.6,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143145029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0