Algal Research-Biomass Biofuels and Bioproducts最新文献_第6页

The effect and mechanism of Synechococcus sp. PCC 7942 harboring VP26 in the immunity of Litopenaeus vannamei against WSSV 含VP26的聚球菌PCC 7942对凡纳滨对虾免疫WSSV的作用及机制

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2026-01-19 DOI: 10.1016/j.algal.2026.104532

Xiang Ding , Shengyu Liao , Chan Zhu , Xiaofeng Gao , Hao Xing , Rui Jia

White spot syndrome virus (WSSV) has become one of the most widely spread deadly viruses in shrimp farming because of its wide host range, fast spreading speed, and high lethality. Synechococcus sp. PCC 7942 is a safe, edible, noncytotoxic cyanobacterium and is a good expression vector. As a tegument protein of WSSV, VP26 plays an important role in the stage of virus reproduction. This study is mainly about constructing Synechococcus sp. PCC 7942 harboring VP26, as well as detecting the expression of host immune pathway marker genes (Dorsal, Relish, STAT, c-Jun, and ALF1), metabolic enzyme activities including lysozyme (LZM), superoxide dismutase (SOD), phenoloxidase (PO), catalase (CAT), glutamate dehydrogenase (GDH), and fatty acid synthase (FAS), and mortality in vivo to evaluate its prevention and treatment effect on WSSV. The study showed that the oral administration of transformed Synechococcus sp. PCC 7942 followed by the challenge led to a relative survival rate of 80% compared with 0% of the positive group. The immune pathway marker gene expression levels and activity of 5 metabolic enzymes tested in the experiment group and the positive group showed an upward trend and reached a peak at 12 h, after which they decreased within 12–24 h. Nevertheless, the activity of LZM in these groups increased within 0–6 h, peaked at 6 h, then decreased. In all the expression and enzyme activity tests, the results in the experiment group were lower than those in the positive group. These results indicated that Synechococcus sp. PCC 7942 harboring VP26 had a positive effect on protecting shrimp from WSSV.

白斑综合征病毒（WSSV）因其宿主范围广、传播速度快、致死率高，已成为对虾养殖中传播最广泛的致命病毒之一。聚藻球菌（Synechococcus sp. pcc7942）是一种安全、可食用、无细胞毒性的蓝藻，是一种良好的表达载体。VP26作为WSSV的被膜蛋白，在病毒繁殖阶段起着重要的作用。本研究主要通过构建携带VP26基因的聚球菌PCC 7942，检测宿主免疫途径标记基因（Dorsal、穗、STAT、c-Jun、ALF1）的表达，体内溶菌酶（LZM）、超氧化物歧化酶（SOD）、酚氧化酶（PO）、过氧化氢酶（CAT）、谷氨酸脱氢酶（GDH）、脂肪酸合成酶（FAS）等代谢酶活性及致死率，评价其对WSSV的防治效果。研究表明，口服转化聚球菌PCC 7942后攻毒，相对存活率为80%，阳性组为0%。试验组和阳性组免疫通路标记基因表达水平和5种代谢酶活性均呈上升趋势，在12 h达到峰值，12 - 24 h内下降，而各组LZM活性在0-6 h内升高，6 h达到峰值，然后下降。在所有的表达和酶活性测试中，实验组的结果低于阳性组。上述结果表明，含VP26的聚球菌PCC 7942对WSSV有一定的保护作用。

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引用次数: 0

From linear to circular bioeconomy: Case study and life cycle assessment of integrating small-scale freshwater aquaculture with microalgae cultivation 从线性到循环生物经济：小规模淡水养殖与微藻养殖相结合的案例研究与生命周期评估

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2026-01-19 DOI: 10.1016/j.algal.2026.104531

Alexander Meneses-Jácome , Sebastián Muñoz-Chinome , Lorena Durán-Ortiz , Astrid Y. Rincón-Hernández , Yohana Castro-Hernández

This study deals with the use of microalgae cultivation system to treat effluents from a small, artisanal fish farm that breed “red tilapia” in Colombia. These types of “aquafarms” have limited technology and high production costs, particularly regarding aquaculture feed inputs. The proposed integrated “aquaculture-microalgae” process aims to recover nutrients and rationalise freshwater intake, whilst the harvested algae biomass partially offsets commercial feed and provides fish farmers with extra income. In this way “circular bioeconomy” notions are brought to this traditional form of aquaculture. The results of this study comprise a life cycle assessment (LCA) based on data extracted from a fully integrated pilot system for fish farming and microalgae (Spirulina) production, using a 6.6 m³ open-raceway pond bioreactor; it also covers the validation of aquafeed replacement with microalgae. LCA methodology has provided a more comprehensive view of the environmental trade-offs and co-benefits associated with the proposed shift from a traditional, linear model of aquaculture to a circular bioeconomy, but it also reveals areas requiring attention for its full maturation and potential replication. The environmental midpoint indicators used to analyse local water pollution, climate change and resource use, return lower values in circular bioeconomy scenarios with “enhanced circularity”, than in the baseline scenario (traditional fish farming). Although the circular bioeconomy model and reference scenario display similar toxicity indicator results, positive change is possible in this respect. Lastly, it is suggested using the harvested biomass for basic “in situ” applications, without major downstream refinement, to be sustainable in isolated, low-income contexts.

本研究涉及使用微藻培养系统来处理哥伦比亚一个养殖“红罗非鱼”的小型手工养鱼场的废水。这些类型的“水产养殖场”技术有限，生产成本高，特别是在水产养殖饲料投入方面。拟议的综合“水产养殖-微藻”过程旨在回收营养物质并使淡水摄入量合理化，而收获的藻类生物量部分抵消了商业饲料，并为养鱼户提供了额外的收入。通过这种方式，“循环生物经济”概念被带入了这种传统的水产养殖形式。本研究的结果包括生命周期评估（LCA），该评估基于从一个完全集成的养鱼和微藻（螺旋藻）生产中试系统中提取的数据，该系统使用6.6立方米的开放式沟道池塘生物反应器；它还涵盖了用微藻替代水产饲料的验证。LCA方法对从传统的线性水产养殖模式向循环生物经济模式转变所带来的环境权衡和共同利益提供了更全面的看法，但它也揭示了需要注意的领域，以使其完全成熟和潜在的复制。用于分析当地水污染、气候变化和资源利用的环境中点指标在“增强循环性”的循环生物经济情景中返回的值低于基线情景（传统养鱼）。尽管循环生物经济模型和参考情景显示出相似的毒性指标结果，但在这方面有可能发生积极的变化。最后，建议将收获的生物质用于基本的“就地”应用，而不需要进行主要的下游提纯，以便在孤立的低收入环境中可持续发展。

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引用次数: 0

Research hotspots and evolutionary trends in microalgae-derived EPA production: A bibliometric review 微藻产生EPA的研究热点和进化趋势：文献计量学综述

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2026-01-15 DOI: 10.1016/j.algal.2025.104505

Qingting Weng , Xiaoai Lin , Longzao Luo , Lang Tan , Qin Liu , Shiping Lu , Guosheng Li , Zhou Zhang

In response to the growing demand for sustainable sources of omega-3 fatty acids, this study provides a comprehensive evaluation of academic and patent advances in eicosapentaenoic acid (EPA) production from microalgae. A bibliometric analysis was conducted using VOSviewer on publications retrieved from the Web of Science Core Collection and patent documents from Derwent Innovations Index between 2005 and 2024. Keyword co-occurrence and clustering analyses were employed to identify major research themes and temporal trends. The results reveal a steady increase in annual publications, divided into three distinct phases. Patent analysis shows parallel growth in commercial activity with a 2–3-year lag behind academic research peaks. Research spans diverse disciplines, including marine biology, biotechnology, and microbiology. Articles were published in 200 journals, 24 of which each published more than 10 articles each. The United States and China were identified as the leading countries in both publication output and collaborative influence. Author co-occurrence analysis indicated that Martin J. Kainz was the most prolific contributor. High-frequency keywords such as “microalgae”, “growth”, and “EPA” reflect central research themes, with clusters highlighting biological traits and environmental factors. Current research focuses on strain selection, lipid composition, metabolic engineering, and commercial applications. Microalgae-derived EPA represents a promising sustainable alternative to fish oil, with significant potential in nutrition, aquaculture, and the food industry.

为了响应对omega-3脂肪酸可持续来源日益增长的需求，本研究对微藻生产二十碳五烯酸（EPA）的学术和专利进展进行了全面评估。利用VOSviewer对2005 - 2024年间Web of Science核心馆藏的出版物和Derwent Innovations Index的专利文献进行了文献计量分析。关键词共现和聚类分析用于确定主要研究主题和时间趋势。结果显示，年度出版物稳步增长，分为三个不同的阶段。专利分析显示，商业活动的平行增长比学术研究峰值滞后2 - 3年。研究跨越多个学科，包括海洋生物学、生物技术和微生物学。在200种期刊上发表文章，其中24种期刊发表10篇以上。美国和中国被确定为在出版物产出和合作影响力方面领先的国家。作者共现分析表明，Martin J. Kainz是最多产的贡献者。高频关键词如“微藻”、“生长”和“EPA”反映了中心研究主题，集群突出了生物特性和环境因素。目前的研究重点是菌株选择、脂质组成、代谢工程和商业应用。微藻衍生的EPA代表了一种有前途的可持续的鱼油替代品，在营养、水产养殖和食品工业中具有巨大的潜力。

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引用次数: 0

Acetate-enabled staged strategy in Chlorella vulgaris culture: Relieving ammonium inhibition while reducing ammonia stripping and enhancing protein content 醋酸盐激活小球藻培养的阶段性策略：缓解氨抑制，减少氨剥离，提高蛋白质含量

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2026-01-11 DOI: 10.1016/j.algal.2026.104527

Zhongliang Sun, Chenmei Bo, Shuonan Cao, Liqin Sun

High-ammonium dosing effectively suppresses protozoan contamination in Chlorella cultivation but causes growth inhibition, ammonia volatilization, and photosynthetic damage. Developing a sustainable strategy that mitigates these adverse effects while maintaining productivity is critical for large-scale applications. A staged cultivation strategy combining ammonium bicarbonate treatment with acetate-based recovery successfully restored algal growth and nitrogen utilization. Acetate maintained near-neutral pH, reduced ammonia stripping by 41%, and increased biomass productivity by 92% compared with CO₂ control. Chlorophyll a fluorescence revealed faster recovery of F_v/F_m and ψ₀, indicating enhanced PSII function and photochemical efficiency. Acetate alleviates ammonium inhibition through physicochemical buffering and metabolic coordination, stabilizing pH, supplying carbon skeletons for nitrogen assimilation, and supporting thylakoid repair. This dual-function approach provides a simple, cost-effective, and scalable solution for open-pond cultivation, integrating contamination control with improved nitrogen-use efficiency and photosynthetic resilience.

高铵处理能有效抑制小球藻的原生动物污染，但会造成生长抑制、氨挥发和光合损伤。开发一种可持续的策略，在保持生产力的同时减轻这些不利影响，这对于大型应用程序至关重要。碳酸氢铵处理与醋酸盐回收相结合的分阶段培养策略成功地恢复了藻类的生长和氮的利用。与CO2对照相比，乙酸保持了接近中性的pH值，减少了41%的氨溶出，提高了92%的生物量生产力。叶绿素a荧光显示Fv/Fm和ψ 0恢复更快，表明PSII功能和光化学效率增强。乙酸通过物理化学缓冲和代谢协调、稳定pH、为氮同化提供碳骨架和支持类囊体修复来减轻铵的抑制作用。这种双重功能的方法为露天池塘养殖提供了一种简单、经济、可扩展的解决方案，将污染控制与提高氮利用效率和光合恢复能力相结合。

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引用次数: 0

Optimizing electrode-integrated microalgae cultivation systems for simultaneous bioelectricity and lipid production 优化电极集成微藻培养系统，同时进行生物电和脂质生产

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2026-01-10 DOI: 10.1016/j.algal.2026.104526

Amina Lami Mohammed , Man Kee Lam , Uganeeswary Suparmaniam , Jun Wei Lim , Inn Shi Tan , Sie Yon Lau , Bridgid Lai Fui Chin , Peck Loo Kiew

Microalgae are well-known for their high lipid content, which can be converted into biodiesel, providing a renewable energy source. This study investigates the potential of microalgae to generate bioelectricity during cultivation, thereby providing an additional renewable energy source prior to biodiesel production. The research systematically investigates the effects of electrode material, pH, nutrient concentration and salinity on the growth, lipid accumulation and electrochemical performance of Chlorella vulgaris. The optimal performance was achieved using AlZn electrodes at pH 5.0, a nutrient concentration of 8% v/v and a salinity of 0.2M. Under these conditions, both lipid production and bioelectricity generation were enhanced, resulting in the highest bioelectricity output of 0.82 V and a lipid content of 35.5% wt., corresponding to the maximum lipid yield of 0.302 g/L. These findings demonstrate the dual benefit of high lipid yield and bioelectricity generation, underscoring the potential of electrode-integrated microalgae cultivation systems for the simultaneous production of both biofuels and bioelectricity, indicating their applicability as scalable and sustainable platforms for renewable energy solutions. This study highlights the synergistic enhancement of biofuel production and bioelectricity generation, emphasizing the importance of optimizing cultivation parameters for efficient and sustainable energy production.

微藻以其高脂含量而闻名，可以转化为生物柴油，提供可再生能源。本研究调查了微藻在培养过程中产生生物电的潜力，从而在生物柴油生产之前提供额外的可再生能源。本研究系统考察了电极材料、pH、营养浓度和盐度对普通小球藻生长、脂质积累和电化学性能的影响。在pH为5.0、营养物质浓度为8% v/v、盐度为0.2M的条件下，AlZn电极的性能最佳。在这些条件下，脂质产量和生物发电量都得到了提高，最高的生物电输出为0.82 V，脂质含量为35.5% wt.，对应的最大脂质产量为0.302 g/L。这些发现证明了高脂质产量和生物发电的双重好处，强调了电极集成微藻培养系统同时生产生物燃料和生物发电的潜力，表明它们作为可再生能源解决方案的可扩展和可持续平台的适用性。该研究强调了生物燃料生产和生物发电的协同增强，强调了优化栽培参数对高效和可持续能源生产的重要性。

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引用次数: 0

Seaweed as a functional feed additive in aquaculture: Efficacy, safety, and fish species-specific optimization 海藻作为水产养殖功能性饲料添加剂：功效、安全性和鱼类特异性优化

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2026-01-10 DOI: 10.1016/j.algal.2026.104525

Sahr Lamin Sumana , Gnoumasse Sidibe , Ahmed E. Elshafey , Damien Habinshuti , Agnes Mattia , Jusu Massaquoi , Sheku Tarawallie , Mukhtar Muhammad Abdullateef , Abubakar Shuaibu , Prince Tongor Mabey , Amidu Mansaray , Yang Xiaofei , Jiang Ruitong , Abdul Karim Meinday , Samuel Ayeh Osei , Joseph Saidu Sankoh , Alie Basma Conteh , Abdul Salami Bah , Md Mohidul Islam , Chernor A.U. Bah , Jianheng Zhang

This review finds that incorporating seaweed into aquafeeds can improve growth, immunity, and gut health, but its effectiveness is highly dependent on the fish species and the dosage used. The results show that incorporating seaweed into aquafeeds generally enhances fish growth, feed efficiency, immunity, metabolic activity, and gut health when used at low to moderate inclusion levels. Many species especially marine carnivores like Sparus aurata and omnivores like Oreochromis niloticus exhibit improved specific growth rate, stronger antioxidant enzyme activity, healthier intestinal structure, and more favorable gut microbiota profiles. Immune responses, including increased lysozyme, SOD, and cytokine expression, are consistently reported. However, low to moderate levels (e.g., 5–15% of the whole meal) are beneficial for many species, especially marine carnivores, while herbivores can tolerate higher levels. However, excessive inclusion can be harmful, impairing digestion and causing stress. Key safety concerns include the potential for seaweeds to accumulate heavy metals and iodine, requiring careful sourcing and processing. When applied judiciously with species-specific formulations, seaweeds are a viable functional ingredient that can enhance aquaculture sustainability, reduce environmental impact, and improve biosecurity.

本综述发现，将海藻添加到水产饲料中可以改善生长、免疫和肠道健康，但其效果高度依赖于鱼的种类和使用的剂量。结果表明，在水产饲料中添加低至中等水平的海藻可促进鱼类生长、饲料效率、免疫力、代谢活性和肠道健康。许多物种，特别是像Sparus aurata这样的海洋食肉动物和像Oreochromis niloticus这样的杂食动物，表现出更高的特定生长率，更强的抗氧化酶活性，更健康的肠道结构和更有利的肠道微生物群。免疫反应，包括增加溶菌酶，SOD和细胞因子的表达，一致报道。然而，低至中等水平（例如，整个膳食的5-15%）对许多物种都是有益的，特别是海洋食肉动物，而食草动物可以忍受更高的水平。然而，过量的含糖是有害的，会损害消化并造成压力。主要的安全问题包括海藻可能积聚重金属和碘，需要仔细采购和加工。如果合理使用特定品种的配方，海藻是一种可行的功能成分，可以增强水产养殖的可持续性，减少环境影响，并改善生物安全。

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引用次数: 0

Cold adaptation mechanisms in Heterococcus viridis: Insights from morphological, physiological, and transcriptomic analyses 绿异球菌的冷适应机制：来自形态学、生理学和转录组学分析的见解

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2026-01-10 DOI: 10.1016/j.algal.2026.104524

Qian Deng , Yongxin Zhang , Ziqing Liu , Bohan Yu , Chengwu Zhang , Miao Cui , Baoyan Gao

Psychrotolerant and psychrophilic microalgae have developed diverse strategies to survive in cold environments through long-term adaptation and evolution. In this study, we examined the cold-tolerance mechanisms of the filamentous yellow-green alga Heterococcus viridis at morphological, physiological, and transcriptomic levels. Our findings show that H. viridis exhibits a complex life cycle involving multiple cellular morphologies, including zoosporangia, zoospores, vegetative cells, coccoid cell clusters, and irregularly branching filaments. When shifted from ambient temperature to 5 ± 1 °C, H. viridis exhibited marked physiological changes: motile zoospore formation was strongly induced, total lipid content increased considerably during the later stages of cold acclimation, while total protein and carbohydrate contents decreased. Transcriptomic analysis revealed that genes associated with flagella, cilia, and motility were upregulated during zoospore formation. During cold acclimation, key genes involved in polyunsaturated fatty acid synthesis were significantly upregulated, along with genes encoding enzymes of the phosphatidylinositol signaling pathway, including protein kinase C and calcium-dependent protein kinase. Cold-shock protein-related DNA domains were activated, whereas heat-shock protein-related genes were suppressed. Together, these regulatory responses clarify the survival strategies of H. viridis under cold stress, underscoring the complex interplay of morphological plasticity, metabolic transformation, and gene expression regulation that supports cold tolerance.

耐冷和亲冷微藻通过长期的适应和进化，形成了在寒冷环境中生存的多种策略。在这项研究中，我们从形态、生理和转录组学水平研究了丝状黄绿藻绿异球菌的耐冷机制。我们的研究结果表明，H. viridis具有复杂的生命周期，涉及多种细胞形态，包括动物孢子囊、动物孢子、营养细胞、球虫细胞簇和不规则分支细丝。当环境温度升高到5±1℃时，绿芽胞杆菌表现出明显的生理变化：运动游动孢子的形成被强烈诱导，总脂质含量在冷驯化后期显著增加，总蛋白和总碳水化合物含量下降。转录组学分析显示，与鞭毛、纤毛和运动性相关的基因在游动孢子形成过程中上调。在冷驯化过程中，参与多不饱和脂肪酸合成的关键基因，以及编码磷脂酰肌醇信号通路酶的基因，包括蛋白激酶C和钙依赖性蛋白激酶，均显著上调。冷休克蛋白相关的DNA结构域被激活，而热休克蛋白相关的基因被抑制。综上所述，这些调控反应阐明了冷胁迫下绿僵菌的生存策略，强调了形态可塑性、代谢转化和基因表达调控之间复杂的相互作用，支持了冷耐受性。

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引用次数: 0

Selenium accumulation in Arthrospira platensis under sulfur-limited conditions: modulation of selenium/sulfur molar ratio 限硫条件下platarthrospira的硒积累：硒/硫摩尔比的调节

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2026-01-10 DOI: 10.1016/j.algal.2026.104523

Yue Zhou , Jian Zhou , Bin Li , Yajie Xiao , Fuhua Peng , Leipeng Cao

The growth, chlorophyll a, carotenoid, and protein content of Arthrospira platensis (A. platensis) showed a dose-dependent decrease as the selenium-to-sulfur (Se/S) molar ratio increased from 2.0 to 16.0. The optimal Se/S molar ratio for biomass production and organic Se accumulation was found to be 4.0, yielding 0.80 g/L of biomass and 0.38 mg/g of organic Se (based on dried cell weight (DCW) at Se concentration of 40 mg/L. At Se/S molar ratio of 2.0–8.0, A. platensis responded to Se-induced oxidative stress by significantly upregulating the activities of key antioxidant enzymes-SOD, CAT, and GSH-Px, intracellular levels of ROS and MDA showed a slight increase, leading to uncontrolled accumulation of reactive oxygen species and consequent severe damage to the cell membrane. Proteomic analysis revealed 74 up-regulated and 98 down-regulated differentially expressed proteins (DEPs) between the Se/S molar ratio of 4.0 and the control (Se/S molar ratio of 0.19). The down-regulated DEPs were associated with photosynthesis, glycolysis, amino acid biosynthesis, oxidative stress response, and ribosomal structure, correlating with the observed reduction in biomass and biochemical synthesis. In contrast, up-regulated DEPs related to membrane transport, translation, and metal-binding proteins provided a mechanistic explanation for the enhanced organic selenium enrichment. This study clarifies the biological effects of Se/S molar ratio on the growth, Se speciation, and biochemical composition of A. platensis, and provides valuable insights for future genetic engineering approaches aimed at optimizing organic Se accumulation.

随着硒硫摩尔比（Se/S）从2.0增加到16.0,platensis （a . platensis）的生长、叶绿素a、类胡萝卜素和蛋白质含量呈剂量依赖性降低。在硒浓度为40 mg/L时，生物量和有机硒积累的最佳Se/S摩尔比为4.0，产量为0.80 g/L，有机硒产量为0.38 mg/g（基于干细胞重（DCW））。在Se/S摩尔比为2.0 ~ 8.0时，平斑黄芪对硒诱导的氧化应激反应主要表现为sod、CAT和GSH-Px等关键抗氧化酶活性的显著上调，细胞内ROS和MDA水平轻微升高，导致活性氧积累失控，对细胞膜造成严重损伤。蛋白质组学分析显示，Se/S摩尔比为4.0与对照组（Se/S摩尔比为0.19）差异表达蛋白（DEPs）上调74个，下调98个。下调的DEPs与光合作用、糖酵解、氨基酸生物合成、氧化应激反应和核糖体结构有关，与观察到的生物量和生化合成减少有关。相比之下，与膜运输、翻译和金属结合蛋白相关的DEPs的上调为有机硒的富集提供了机制解释。本研究阐明了硒/硫摩尔比对平叶藻生长、硒形态形成和生化组成的生物学影响，为今后优化有机硒积累的基因工程方法提供了有价值的见解。

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引用次数: 0

Elucidating the growth effects of Klebsormidium sp. and Vischeria stellata on maize using network pharmacology and experimental validation 利用网络药理学和实验验证研究克雷伯sordium sp.和Vischeria stellata对玉米生长的影响

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2026-01-07 DOI: 10.1016/j.algal.2026.104519

Yu Jiang , Hao Ning , Min Liu , Zhixin Wei , Min Wu , Xingyu Yan , Peihong Shen , Jian Jin , Luodong Huang

This study investigated the synergistic effects of diverse active compounds in the aqueous extracts of the filamentous alga Klebsormidium sp. (GXU-A8) and the single-celled alga Vischeria stellata (GXU-A13) on maize growth promotion. Using sodium nitrate as the nitrogen source at an initial concentration of 9 mmol/L, GXU-A8 biomass reached 4.73 g/L, while GXU-A13 biomass reached 7.96 g/L. Water-soluble metabolic components were extracted from the obtained biomass of both microalgae. The aqueous extracts of GXU-A8 and GXU-A13 contained 397 and 504 metabolic compounds, respectively, with 124 compounds shared between them. Notably, GXU-A8 was characterized by Linamarin (5.75 %) and GXU-A13 by Choline Alfoscerate (3.36 %). Network analysis revealed that GXU-A8 activated growth-related pathways (chromosome regulation, protein folding, signaling), while GXU-A13 enhanced stress resistance through distinct pathways (terpenoid/polyketide metabolism, photosynthetic carbon fixation, nucleotide metabolism). Molecular docking confirmed key target interactions for each strain (Estriol for GXU-A8, Guanine/Arabinosylhypoxanthine for GXU-A13), consistent with the respective roles of GXU-A8 in promoting growth under non-stress conditions and GXU-A13 in enhancing stress Protection. This study applied network pharmacology and molecular docking to elucidate microalgae-plant interactions for the precise application of bio-stimulant.

研究了丝状藻Klebsormidium sp. （GXU-A8）和单细胞藻stellata Vischeria stellata （GXU-A13）水提液中多种活性成分对玉米生长的协同促进作用。以硝酸钠为氮源，初始浓度为9 mmol/L时，GXU-A8生物量达到4.73 g/L， GXU-A13生物量达到7.96 g/L。从两种微藻获得的生物量中提取水溶性代谢成分。GXU-A8和GXU-A13的水提液分别含有397和504种代谢化合物，两者共有124种代谢化合物。值得注意的是，GXU-A8由亚麻amarin（5.75%）和GXU-A13由胆碱Alfoscerate（3.36%）表征。网络分析表明，GXU-A8激活了生长相关的途径（染色体调节、蛋白质折叠、信号传导），而GXU-A13通过不同的途径（萜类/聚酮代谢、光合碳固定、核苷酸代谢）增强了抗逆性。分子对接确认了各菌株的关键靶点相互作用（GXU-A8为雌三醇，GXU-A13为鸟嘌呤/阿拉伯糖基次黄嘌呤），与GXU-A8在非胁迫条件下促进生长和GXU-A13增强胁迫保护的作用相一致。本研究应用网络药理学和分子对接技术，阐明微藻与植物的相互作用，为生物刺激素的精准应用提供依据。

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引用次数: 0

Diurnal oscillation of prostaglandin pathway gene expression in Skeletonema marinoi under different environmental conditions 不同环境条件下马里诺骨骼肌前列腺素通路基因表达的日波动

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2026-01-07 DOI: 10.1016/j.algal.2026.104521

Laura Vitale , Antonio Fuentes-Lema , Rosa Maria Sepe , Heethaka Krishantha Sameera de Zoysa , Dario Righelli , Cristina Sobrino , Giovanna Romano , Valeria Di Dato

Prostaglandins (PGs) are bioactive lipid compounds involved in physiological and environmental responses in various organisms. While their biosynthesis has been well studied in animals, recent evidence highlighted that diatoms also possess the genetic machinery for PGs synthesis; however, their function remains largely unexplored. This study investigates the diurnal oscillation of PGs pathway gene expression in Skeletonema marinoi under different environmental conditions, including variations in light intensity, temperature, and CO₂ concentration. Using two distinct strains, FE7 and FE60, we analysed the expression dynamics of three key enzymes involved in the prostaglandin biosynthesis: cyclooxygenase-1 (COX-1), microsomal prostaglandin E synthase (PTGES), and prostaglandin-H2 D-isomerase (PTGDS). Our results indicate strain-specific responses to environmental conditions that significantly altered the expression profiles of all three genes, with FE7 exhibiting greater sensitivity to temperature and light fluctuations compared to FE60. High CO₂ levels appear to positively influence the expression of COX and PTGDS and negatively those of PTGES, suggesting a potential role in modulating the production of different PGs, although the function of PGs in diatoms remains unclear. These findings provide preliminary insights into the molecular response of diatoms to environmental changes, contribute to exploring the potential role of PGs in diatoms, and highlight their potential ecological significance in phytoplankton communities, which remains speculative without functional assays and metabolite profiling by LC-MS/MS. To strengthen these findings, future studies should correlate gene expression with physiological indicators such as growth rate, cell morphology, and stress markers.

前列腺素是一种生物活性脂质化合物，参与多种生物的生理和环境反应。虽然它们的生物合成已经在动物中得到了很好的研究，但最近的证据强调，硅藻也具有合成pg的遗传机制；然而，它们的功能在很大程度上仍未被探索。本研究研究了不同光照、温度、CO₂浓度等环境条件下马里诺骨骼肌PGs通路基因表达的日变化。利用两株不同菌株FE7和FE60，分析了参与前列腺素生物合成的三种关键酶：环氧化酶-1 （COX-1）、微体前列腺素E合成酶（PTGES）和前列腺素h2 d异构酶（PTGDS）的表达动态。我们的研究结果表明，菌株对环境条件的特异性反应显著改变了所有三个基因的表达谱，与FE60相比，FE7对温度和光照波动表现出更大的敏感性。高CO₂水平似乎正影响COX和PTGDS的表达，负影响PTGES的表达，这表明在调节不同PGs的产生中可能起作用，尽管PGs在硅藻中的功能尚不清楚。这些发现为硅藻对环境变化的分子响应提供了初步的见解，有助于探索PGs在硅藻中的潜在作用，并强调了它们在浮游植物群落中的潜在生态意义，但在没有LC-MS/MS功能分析和代谢物分析的情况下，这些研究仍处于推测阶段。为了加强这些发现，未来的研究应该将基因表达与生理指标（如生长速度、细胞形态和应激标志物）联系起来。

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引用次数: 0