Algal Research-Biomass Biofuels and Bioproducts最新文献_第4页

Maternal inheritance of photoperiodic responsiveness controls sporophyte maturation in Saccharina japonica: Implications for hybrid kelp breeding and seedling production 母系光周期响应遗传控制日本糖藻孢子体成熟：对杂交海带育种和幼苗生产的启示

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2025-12-23 DOI: 10.1016/j.algal.2025.104493

Jing Li , Xiaodong Li , Li Su , Lirong Chang , Yan Zhang , Ying Sun , Luyang Xiao , Ershuai Huang , Shaojun Pang

Reproductive timing in Saccharina japonica is tightly regulated by photoperiod, yet the genetic mechanism underlying variation in photoperiodic responsiveness remains unclear. This study examined whether photoperiod-dependent sporogenesis is maternally inherited in hybrid sporophytes. Two female gametophyte lines with contrasting maturation phenotypes—early-maturing (day-neutral) and late-maturing (short-day dependent)—were crossed with 22 genetically distinct male gametophytes. The resulting hybrid sporophytes were cultured under both natural and short-day conditions to evaluate reproductive differentiation. Hybrids derived from the early-maturing maternal line developed sori under natural photoperiods, whereas those from the late-maturing line required short-day induction to initiate sorus formation. Histological observations confirmed complete sorus morphogenesis and the release of viable meiospores following short-day exposure. These findings provide direct evidence that photoperiodic responsiveness in S. japonica is genetically determined and transmitted through the maternal lineage. This discovery reveals a novel mechanism of reproductive control in kelps and provides a conceptual framework for integrating photoperiod genetics into the development of cultivars with synchronized reproductive timing for commercial aquaculture.

甘蔗的生殖时间受到光周期的严格调控，但光周期响应性变异的遗传机制尚不清楚。本研究考察了杂交孢子体中依赖光周期的孢子发生是否由母系遗传。将早熟（昼中性）和晚熟（昼短依赖性）两种成熟表型不同的雌性配子体系与22个遗传上不同的雄性配子体杂交。杂种孢子体在自然和短日照条件下培养，以评估生殖分化。来自早熟母系的杂种在自然光周期下形成sori，而来自晚熟系的杂种则需要短时间的诱导才能形成sori。组织学观察证实，在短时间暴露后，孢子形成完整，并释放出活的减数孢子。这些发现提供了直接证据，表明日本稻的光周期反应是由遗传决定的，并通过母系传播。这一发现揭示了一种新的海带繁殖控制机制，并为将光周期遗传学整合到商业养殖中具有同步繁殖时间的品种的开发提供了一个概念框架。

{"title":"Maternal inheritance of photoperiodic responsiveness controls sporophyte maturation in Saccharina japonica: Implications for hybrid kelp breeding and seedling production","authors":"Jing Li , Xiaodong Li , Li Su , Lirong Chang , Yan Zhang , Ying Sun , Luyang Xiao , Ershuai Huang , Shaojun Pang","doi":"10.1016/j.algal.2025.104493","DOIUrl":"10.1016/j.algal.2025.104493","url":null,"abstract":"<div><div>Reproductive timing in <em>Saccharina japonica</em> is tightly regulated by photoperiod, yet the genetic mechanism underlying variation in photoperiodic responsiveness remains unclear. This study examined whether photoperiod-dependent sporogenesis is maternally inherited in hybrid sporophytes. Two female gametophyte lines with contrasting maturation phenotypes—early-maturing (day-neutral) and late-maturing (short-day dependent)—were crossed with 22 genetically distinct male gametophytes. The resulting hybrid sporophytes were cultured under both natural and short-day conditions to evaluate reproductive differentiation. Hybrids derived from the early-maturing maternal line developed sori under natural photoperiods, whereas those from the late-maturing line required short-day induction to initiate sorus formation. Histological observations confirmed complete sorus morphogenesis and the release of viable meiospores following short-day exposure. These findings provide direct evidence that photoperiodic responsiveness in <em>S. japonica</em> is genetically determined and transmitted through the maternal lineage. This discovery reveals a novel mechanism of reproductive control in kelps and provides a conceptual framework for integrating photoperiod genetics into the development of cultivars with synchronized reproductive timing for commercial aquaculture.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"93 ","pages":"Article 104493"},"PeriodicalIF":4.5,"publicationDate":"2025-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145836704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulation of vegetative propagation by exogenous α-NAA and GA1 in Sargassum hemiphyllum var. chinense 外源α-NAA和GA1对马尾草无性繁殖的调控

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2025-12-23 DOI: 10.1016/j.algal.2025.104497

Yuming Wu , Qiong Wu , Yi Luo , Muhamad Syaifudin , Xianming Tang , Tangcheng Li , Jing Chen , Hong Du

Sargassum hemiphyllum var. chinense is a brown seaweed along the southeastern coast of China, playing a significant ecological role and possessing considerable resource utilization value. α-Naphthaleneacetic acid (α-NAA) and Gibberellin acid 1 (GA1) play an important role in S. hemiphyllum var. chinense holdfast regeneration. However, their regulatory mechanisms remain poorly understood. This study investigated the application of α-NAA and GA1 to promote the rapid regeneration from S. hemiphyllum var. chinense holdfast and further explored its mechanism. Compared to the control, treatment with 1.5 mg·L⁻¹ α-NAA and 1.5 mg·L⁻¹ GA1 significantly reduced the regeneration time by a minimum of four days. The average maximum length of the regenerated leaves was approximately 1.79 times that of the control group, and the leaves exhibited propagules induction capacity. During this developmental process, the transcriptome results showed that upregulated enzymes in both the IPA (Indole-3-Pyruvic Acid) and TAM (Tryptamine) branches of tryptophan metabolism elevate IAA levels, with each pathway acting at distinct stages. Meanwhile, the upregulation of CYP735 A (Cytochrome P450 735 A) significantly increased the levels of 2MeScZ, IP, K, and mT. These cytokinins via the up-regulated CRE1 and B-ARR, ultimately promoting cell division. The GAs (GA13919) showed a very high upregulated level, suggesting the potential importance in the development of Sargassum. Thus, exogenous induction with α-NAA and GA1 can solve the problem of scarce juvenile production from S. hemiphyllum var. chinense holdfasts. This approach promotes rapid juvenile regeneration and improves production efficiency. These findings will contribute to the advancement of artificial propagation in macroalgae.？

马尾藻（Sargassum hemiphyllum var. chinense）是中国东南沿海的一种褐藻，具有重要的生态作用，具有相当的资源利用价值。α-萘乙酸（α-NAA）和赤霉素酸1 （GA1）在赤霉素固结再生中起重要作用。然而，它们的调控机制仍然知之甚少。本研究研究了α-NAA和GA1对赤霉素快速再生的促进作用，并进一步探讨了其作用机制。与对照相比，1.5 mg·L−1 α-NAA和1.5 mg·L−1 GA1处理显著缩短了再生时间至少4天。再生叶片的平均最大长度约为对照组的1.79倍，叶片具有繁殖体诱导能力。在这一发育过程中，转录组研究结果显示，色氨酸代谢的IPA（吲哚-3-丙酮酸）和TAM（色氨酸）分支的上调酶均可提高IAA水平，且每种途径在不同的阶段起作用。同时，上调CYP735 A （Cytochrome P450 735 A）可通过上调CRE1和B-ARR，显著提高2MeScZ、IP、K和mT等细胞分裂素的水平，最终促进细胞分裂。GAs （GA13919）显示出非常高的上调水平，表明其在马尾藻发育中的潜在重要性。由此可见，α-NAA和GA1的外源诱导可以解决南水北流苗木幼鱼产量不足的问题。这种方法促进了幼鱼的快速再生，提高了生产效率。这些发现将有助于推进大型藻人工繁殖。

{"title":"Regulation of vegetative propagation by exogenous α-NAA and GA1 in Sargassum hemiphyllum var. chinense","authors":"Yuming Wu , Qiong Wu , Yi Luo , Muhamad Syaifudin , Xianming Tang , Tangcheng Li , Jing Chen , Hong Du","doi":"10.1016/j.algal.2025.104497","DOIUrl":"10.1016/j.algal.2025.104497","url":null,"abstract":"<div><div><em>Sargassum hemiphyllum</em> var. <em>chinense</em> is a brown seaweed along the southeastern coast of China, playing a significant ecological role and possessing considerable resource utilization value. α-Naphthaleneacetic acid (α-NAA) and Gibberellin acid 1 (GA1) play an important role in <em>S. hemiphyllum</em> var. <em>chinense</em> holdfast regeneration. However, their regulatory mechanisms remain poorly understood. This study investigated the application of α-NAA and GA1 to promote the rapid regeneration from <em>S. hemiphyllum</em> var. <em>chinense</em> holdfast and further explored its mechanism. Compared to the control, treatment with 1.5 mg·L<sup>−1</sup> α-NAA and 1.5 mg·L<sup>−1</sup> GA1 significantly reduced the regeneration time by a minimum of four days. The average maximum length of the regenerated leaves was approximately 1.79 times that of the control group, and the leaves exhibited propagules induction capacity. During this developmental process, the transcriptome results showed that upregulated enzymes in both the IPA (Indole-3-Pyruvic Acid) and TAM (Tryptamine) branches of tryptophan metabolism elevate IAA levels, with each pathway acting at distinct stages. Meanwhile, the upregulation of <em>CYP735 A</em> (Cytochrome P450 735 A) significantly increased the levels of 2MeScZ, IP, K, and mT. These cytokinins via the up-regulated <em>CRE1</em> and <em>B-ARR</em>, ultimately promoting cell division. The GAs (GA13919) showed a very high upregulated level, suggesting the potential importance in the development of <em>Sargassum</em>. Thus, exogenous induction with α-NAA and GA1 can solve the problem of scarce juvenile production from <em>S. hemiphyllum</em> var. <em>chinense</em> holdfasts. This approach promotes rapid juvenile regeneration and improves production efficiency. These findings will contribute to the advancement of artificial propagation in macroalgae.？</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"94 ","pages":"Article 104497"},"PeriodicalIF":4.5,"publicationDate":"2025-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145973858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a scalable design for biofuel production from green macroalgae hydrolysates 从绿色大型藻类水解物生产生物燃料的可扩展设计的发展

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2025-12-22 DOI: 10.1016/j.algal.2025.104487

Hayet Djelal , Alaa Salma , Walaa Sayed , Audrey Cabrol , Maud Benoit , Amance Corat , Abdeltif Amrane , Ronan Pierre

This study investigated the valorization of green macroalgae in ethanol by the yeast Saccharomyces cerevisiae. The proof of concept was conducted with two green macroalgae hydrolysates, namely Ulva rigida and Chaetomorpha linum and the fermentation process was scaled-up from 250 mL and 500 mL shake flasks to a 3 L stirred-tank bioreactor. Firstly, the experiments used a synthetic medium based on algal hydrolysate, examining factors like nitrogen source, inoculum size, and salt content. After 72 h, ethanol yields were similar across the systems: 0.43 g.g⁻¹ in the bioreactor, compared to 0.46 and 0.45 g.g⁻¹ in 250 mL and 500 mL flasks, respectively. Secondly, fermenting green algae hydrolysate was tested, under similar conditions (without aeration and pH adjustment, with nitrogen enrichment, and 1 % v/v inoculum) resulted in an ethanol concentration of 9.05 g.L⁻¹, a yield of 0.44 g.g⁻¹, and 85.86 % efficiency. Thirdly, operating the process at larger scale was carried out on pilot scale of 77 L with Chaetomorpha linum hydrolysate. Glucose was fully consumed in 9 h, and maximum ethanol production (5.61 g.L⁻¹) occurred after 11 h, with a yield of 0.52 g.g⁻¹ which corresponds to the theoretical yield.

本文研究了绿色巨藻在乙醇中的发酵过程。概念验证是用两种绿色大型藻类水解物进行的，即刚性Ulva和毛藻（Chaetomorpha linum），并将发酵过程从250 mL和500 mL摇瓶放大到3 L搅拌槽生物反应器。首先，采用以藻类水解物为基础的合成培养基，考察氮源、接种量、含盐量等因素。72h后，不同体系的乙醇产量相似：生物反应器中的乙醇产量为0.43 g g−1，而250 mL和500 mL烧瓶中的乙醇产量分别为0.46 g g−1和0.45 g g−1。其次，对绿藻水解液进行发酵，在相同的条件下（不曝气，不调节pH，富氮，接种量为1% v/v），乙醇浓度为9.05 g。L−1，产率为0.44 g.g−1，效率为85.86%。再次，以毛藻水解液为原料，在77 L的中试规模上进行了该工艺的大规模操作。葡萄糖在9 h内完全消耗，最大乙醇产量为5.61 g。L−1)在11 h后发生，产率为0.52 g.g−1，符合理论产率。

{"title":"Development of a scalable design for biofuel production from green macroalgae hydrolysates","authors":"Hayet Djelal , Alaa Salma , Walaa Sayed , Audrey Cabrol , Maud Benoit , Amance Corat , Abdeltif Amrane , Ronan Pierre","doi":"10.1016/j.algal.2025.104487","DOIUrl":"10.1016/j.algal.2025.104487","url":null,"abstract":"<div><div>This study investigated the valorization of green macroalgae in ethanol by the yeast <em>Saccharomyces cerevisiae</em>. The proof of concept was conducted with two green macroalgae hydrolysates, namely <em>Ulva rigida and Chaetomorpha linum</em> and the fermentation process was scaled-up from 250 mL and 500 mL shake flasks to a 3 L stirred-tank bioreactor. Firstly, the experiments used a synthetic medium based on algal hydrolysate, examining factors like nitrogen source, inoculum size, and salt content. After 72 h, ethanol yields were similar across the systems: 0.43 g.g<sup>−1</sup> in the bioreactor, compared to 0.46 and 0.45 g.g<sup>−1</sup> in 250 mL and 500 mL flasks, respectively. Secondly, fermenting green algae hydrolysate was tested, under similar conditions (without aeration and pH adjustment, with nitrogen enrichment, and 1 % <em>v</em>/v inoculum) resulted in an ethanol concentration of 9.05 g.L<sup>−1</sup>, a yield of 0.44 g.g<sup>−1</sup>, and 85.86 % efficiency. Thirdly, operating the process at larger scale was carried out on pilot scale of 77 L with <em>Chaetomorpha linum</em> hydrolysate. Glucose was fully consumed in 9 h, and maximum ethanol production (5.61 g.L<sup>−1</sup>) occurred after 11 h, with a yield of 0.52 g.g<sup>−1</sup> which corresponds to the theoretical yield.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"94 ","pages":"Article 104487"},"PeriodicalIF":4.5,"publicationDate":"2025-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145923282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insights into leveraging alga–bacterium cooperation for dairy wastewater valorization 利用藻类-细菌合作实现乳制品废水增值的见解

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2025-12-22 DOI: 10.1016/j.algal.2025.104481

Caterina Isabel Manzano-Puga , Nuria Membrives-Barea , Aitor Gómez-Osuna , María Jesús Torres , Alexandra Dubini , David González-Ballester

The integration of microalgae-bacteria consortia into wastewater treatment offers a promising strategy for sustainable biomass production and bioremediation. This study investigates the performance of Chlamydomonas reinhardtii in consortium with Serratia liquefaciens DWW128, isolated from raw dairy wastewater (DWW). The consortium, and respective monocultures were cultivated in both untreated, raw DWW and sterile, simulated DWW (sDWW). C. reinhardtii alone exhibited negligible growth in sDWW, while the native microbial community of raw DWW supported its growth (35.2 μg·mL⁻¹ chlorophyll and 1.6 g·L⁻¹ of biomass). However, native microorganisms also negatively affect the alga by limiting its maximum growth compared to cocultures with S. liquefaciens in raw DWW (53.2 μg·mL⁻¹ chlorophyll, 3.1 g·L⁻¹ biomass) and in sDWW (90 μg·mL⁻¹ chlorophyll, 7.1 g·L⁻¹ biomass). The enhancement of algal growth is more pronounced under aerobic conditions, and the supply of ammonium through bacterial proteolytic activity, and possibly also acetic acid, are key factors for algal growth. Cocultures can reduce up to 57.6 % of the bacterial CO₂ emissions. In contrast, S. liquefaciens did not depend on C. reinhardtii to grow successfully in DWW and can produce a substantial amount of bioH₂ (200.7 mL·L⁻¹) in 48 h. However, the presence of the alga enhances the bacterium's viability and persistence. This study provides valuable insights into harnessing the metabolic specialization of diverse microalgal and bacterial species to design tailored consortia capable of exploiting the broad spectrum of nutrient sources in wastewater, enabling more robust and effective biotechnological applications.

Raw Dataset repository: 10.17632/39ckdr8w2n.1

将微藻-细菌联合体整合到废水处理中为可持续生物质生产和生物修复提供了一种有前途的策略。本研究研究了莱茵衣单胞菌与液化沙雷氏菌DWW128联合处理乳废水的性能。在未经处理的原始DWW和无菌的模拟DWW （sDWW）中培养财团和各自的单培养物。单独的莱因哈蒂草（C. reinhardtii）在sDWW中的生长可以忽略不计，而原始DWW的原生微生物群落支持其生长（35.2 μg·mL−1叶绿素和1.6 g·L−1生物量）。然而，与与S.液化菌共培养相比，原生微生物也会对藻类产生负面影响，在原DWW （53.2 μg·mL−1叶绿素，3.1 g·L−1生物量）和sDWW （90 μg·mL−1叶绿素，7.1 g·L−1生物量）中限制藻类的最大生长。在好氧条件下，藻类生长的增强更为明显，通过细菌蛋白水解活性提供的铵，可能还有乙酸，是藻类生长的关键因素。共培养可以减少高达57.6%的细菌二氧化碳排放量。相比之下，液化链球菌不依赖赖因哈氏c菌在DWW中成功生长，并且在48 h内可以产生大量的bioH2 （200.7 mL·L−1）。然而，藻类的存在增强了细菌的生存能力和持久性。这项研究为利用不同微藻和细菌物种的代谢专一性来设计量身定制的联盟提供了有价值的见解，这些联盟能够利用废水中广泛的营养来源，从而实现更强大和有效的生物技术应用。原始数据库：10.17632/39ckdr8w2n.1

{"title":"Insights into leveraging alga–bacterium cooperation for dairy wastewater valorization","authors":"Caterina Isabel Manzano-Puga , Nuria Membrives-Barea , Aitor Gómez-Osuna , María Jesús Torres , Alexandra Dubini , David González-Ballester","doi":"10.1016/j.algal.2025.104481","DOIUrl":"10.1016/j.algal.2025.104481","url":null,"abstract":"<div><div>The integration of microalgae-bacteria consortia into wastewater treatment offers a promising strategy for sustainable biomass production and bioremediation. This study investigates the performance of <em>Chlamydomonas reinhardtii</em> in consortium with <em>Serratia liquefaciens</em> DWW128, isolated from raw dairy wastewater (DWW). The consortium, and respective monocultures were cultivated in both untreated, raw DWW and sterile, simulated DWW (sDWW). <em>C. reinhardtii</em> alone exhibited negligible growth in sDWW, while the native microbial community of raw DWW supported its growth (35.2 μg·mL<sup>−1</sup> chlorophyll and 1.6 g·L<sup>−1</sup> of biomass). However, native microorganisms also negatively affect the alga by limiting its maximum growth compared to cocultures with <em>S. liquefaciens</em> in raw DWW (53.2 μg·mL<sup>−1</sup> chlorophyll, 3.1 g·L<sup>−1</sup> biomass) and in sDWW (90 μg·mL<sup>−1</sup> chlorophyll, 7.1 g·L<sup>−1</sup> biomass). The enhancement of algal growth is more pronounced under aerobic conditions, and the supply of ammonium through bacterial proteolytic activity, and possibly also acetic acid, are key factors for algal growth. Cocultures can reduce up to 57.6 % of the bacterial CO<sub>2</sub> emissions. In contrast, <em>S. liquefaciens</em> did not depend on <em>C. reinhardtii</em> to grow successfully in DWW and can produce a substantial amount of bioH<sub>2</sub> (200.7 mL·L<sup>−1</sup>) in 48 h. However, the presence of the alga enhances the bacterium's viability and persistence. This study provides valuable insights into harnessing the metabolic specialization of diverse microalgal and bacterial species to design tailored consortia capable of exploiting the broad spectrum of nutrient sources in wastewater, enabling more robust and effective biotechnological applications.</div><div><strong>Raw Dataset repository</strong>: <span><span>10.17632/39ckdr8w2n.1</span><svg><path></path></svg></span></div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"94 ","pages":"Article 104481"},"PeriodicalIF":4.5,"publicationDate":"2025-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145923305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Egg-free mayonnaise-type emulsions stabilized with whole Spirulina biomass: Rheological, structural, and sensory characterization 用整个螺旋藻生物量稳定的无蛋黄酱型乳剂：流变学、结构和感官表征

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2025-12-22 DOI: 10.1016/j.algal.2025.104495

Jikang Sui , Yuxuan Cui , Xiangning Lu , Wenhai She , Kangning Wang , Mingkai Bai , Jianwei Yu , Adewale Olusegun Obadina , Guangxin Feng , Haohao Wu

This study investigates the potential of using whole Spirulina biomass as a sustainable and multifunctional structuring agent for plant-based emulsion gels, specifically targeting the replacement of egg yolk in mayonnaise-like spreads. Various concentrations of Spirulina (ranging from 5.00 % to 11.67 %) and sodium alginate (from 0.15 % to 1.20 %) were tested to formulate both full-fat and low-fat emulsion gels. The results revealed that the full-fat Spirulina emulsion (8.33 % Spirulina) exhibited similar texture, spreadability, and sensory properties to traditional egg yolk mayonnaise, with a sensory score of 82.22 ± 3.22. In the low-fat formulations, fat reduction was achieved by increasing the aqueous phase while incorporating 0.60 %–0.90 % sodium alginate to rebuild continuous-phase viscosity, thereby maintaining a self-supporting, spreadable structure and comparable sensory attributes (score of 80). Rheological measurements showed that the low-fat Spirulina emulsion with 0.60 % sodium alginate had a thixotropic recovery rate of 95.15 %, closely matching that of egg yolk mayonnaise (115.77 %). These findings highlight that whole Spirulina biomass can be effectively used to replace egg yolk in emulsion-based spreads, offering a nutritionally rich, sustainable alternative with both desirable functional and sensory qualities.

本研究探讨了利用整个螺旋藻生物量作为植物基乳液凝胶的可持续和多功能结构剂的潜力，特别是针对蛋黄酱类涂抹中蛋黄的替代。不同浓度的螺旋藻（5.00 %至11.67%）和海藻酸钠（0.15%至1.20%）进行了测试，以配制全脂和低脂乳液凝胶。结果表明，全脂螺旋藻乳液（螺旋藻含量为8.33%）的质地、涂抹性和感官性能与传统蛋黄蛋黄酱相似，感官评分为82.22±3.22。在低脂配方中，脂肪的减少是通过增加水相来实现的，同时加入0.60% - 0.90%的海藻酸钠来重建连续相粘度，从而保持自我支撑，可涂抹的结构和相当的感官属性（得分80）。流变学测试表明，添加0.60%海藻酸钠的低脂螺旋藻乳触变回收率为95.15%，与蛋黄蛋黄酱的触变回收率（115.77%）非常接近。这些研究结果表明，整个螺旋藻生物量可以有效地取代蛋黄，提供一种营养丰富、可持续的替代品，同时具有理想的功能和感官品质。

{"title":"Egg-free mayonnaise-type emulsions stabilized with whole Spirulina biomass: Rheological, structural, and sensory characterization","authors":"Jikang Sui , Yuxuan Cui , Xiangning Lu , Wenhai She , Kangning Wang , Mingkai Bai , Jianwei Yu , Adewale Olusegun Obadina , Guangxin Feng , Haohao Wu","doi":"10.1016/j.algal.2025.104495","DOIUrl":"10.1016/j.algal.2025.104495","url":null,"abstract":"<div><div>This study investigates the potential of using whole Spirulina biomass as a sustainable and multifunctional structuring agent for plant-based emulsion gels, specifically targeting the replacement of egg yolk in mayonnaise-like spreads. Various concentrations of Spirulina (ranging from 5.00 % to 11.67 %) and sodium alginate (from 0.15 % to 1.20 %) were tested to formulate both full-fat and low-fat emulsion gels. The results revealed that the full-fat Spirulina emulsion (8.33 % Spirulina) exhibited similar texture, spreadability, and sensory properties to traditional egg yolk mayonnaise, with a sensory score of 82.22 ± 3.22. In the low-fat formulations, fat reduction was achieved by increasing the aqueous phase while incorporating 0.60 %–0.90 % sodium alginate to rebuild continuous-phase viscosity, thereby maintaining a self-supporting, spreadable structure and comparable sensory attributes (score of 80). Rheological measurements showed that the low-fat Spirulina emulsion with 0.60 % sodium alginate had a thixotropic recovery rate of 95.15 %, closely matching that of egg yolk mayonnaise (115.77 %). These findings highlight that whole Spirulina biomass can be effectively used to replace egg yolk in emulsion-based spreads, offering a nutritionally rich, sustainable alternative with both desirable functional and sensory qualities.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"93 ","pages":"Article 104495"},"PeriodicalIF":4.5,"publicationDate":"2025-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145836703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of domestic wastewater micropollutants on microbial diversity in microalgae-based treatment systems 生活污水微污染物对微藻处理系统中微生物多样性的影响

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2025-12-21 DOI: 10.1016/j.algal.2025.104496

Caroline Moço Erba Pompei , Thaís Garcia da Silva Garcia , Inessa Lacativa Bagatini , Hugo Renan Bolzani , Gustavo Henrique Ribeiro da Silva

Microalgae photobioreactors are excellent low-cost alternatives for natural wastewater treatment with the potential for reuse. Despite the general knowledge on chemical contamination of water by micropollutants, little is known about the adverse effects of these compounds on the health of aquatic biota and on the operation of microalgae-based systems. This study revealed the influence of 12 selected micropollutants in a wastewater (real) treatment by a flat-panel photobioreactor (outdoors) on both the removal of nutrients and the generation of biomass, as well as on the composition of the main communities of the system (microalgae, cyanobacteria and bacteria). The presence of micropollutants severely inhibited microalgal metabolism, causing the total nitrogen and total phosphorus removal efficiency to decrease from 71 % (control) to 32 % and 36 %, respectively. Biomass productivity also decreased from 11.5 mg TSS L⁻¹ d⁻¹ to 9.6 mg TSS L⁻¹ d⁻¹. However, N-NH₄ removal remained high at 88 %, suggesting the functional resilience of ammonia-oxidizing bacteria. Additionally, a predictive metabolic pathway for bacteria was investigated, indicating prevailing ammonia oxidation and dehalogenation. Tetradesmus obliquus was the dominant microalga (>95 %) in the photobioreactors. Although no significant difference was observed in the composition of microalgae during the experiments with and without micropollutants, the presence of the 12 selected micropollutants may have inhibited microalgal metabolism during treatment. In relation to bacteria, Patescibacteria seems to be an indicator of the presence of micropollutants.

微藻光生物反应器是一种极好的低成本、可重复利用的天然废水处理方法。尽管人们普遍了解微污染物对水的化学污染，但对这些化合物对水生生物群健康和微藻系统运行的不利影响知之甚少。本研究揭示了平板光生物反应器（室外）处理废水（真实）时选定的12种微污染物对营养物去除和生物量产生的影响，以及对系统主要群落（微藻、蓝藻和细菌）组成的影响。微污染物的存在严重抑制了微藻的代谢，导致总氮和总磷的去除率分别从71%（对照）下降到32%和36%。生物量生产力也从11.5 mg TSS L−1 d−1下降到9.6 mg TSS L−1 d−1。然而，N-NH4的去除率仍然高达88%，表明氨氧化细菌的功能恢复能力。此外，对细菌的预测代谢途径进行了研究，表明氨气氧化和脱卤作用普遍存在。斜四角藻是光生物反应器中的优势微藻（95%）。虽然在有微污染物和没有微污染物的实验中，微藻的组成没有显著差异，但12种选定的微污染物的存在可能抑制了微藻在处理过程中的代谢。关于细菌，Patescibacteria似乎是微污染物存在的一个指标。

{"title":"Impact of domestic wastewater micropollutants on microbial diversity in microalgae-based treatment systems","authors":"Caroline Moço Erba Pompei , Thaís Garcia da Silva Garcia , Inessa Lacativa Bagatini , Hugo Renan Bolzani , Gustavo Henrique Ribeiro da Silva","doi":"10.1016/j.algal.2025.104496","DOIUrl":"10.1016/j.algal.2025.104496","url":null,"abstract":"<div><div>Microalgae photobioreactors are excellent low-cost alternatives for natural wastewater treatment with the potential for reuse. Despite the general knowledge on chemical contamination of water by micropollutants, little is known about the adverse effects of these compounds on the health of aquatic biota and on the operation of microalgae-based systems. This study revealed the influence of 12 selected micropollutants in a wastewater (real) treatment by a flat-panel photobioreactor (outdoors) on both the removal of nutrients and the generation of biomass, as well as on the composition of the main communities of the system (microalgae, cyanobacteria and bacteria). The presence of micropollutants severely inhibited microalgal metabolism, causing the total nitrogen and total phosphorus removal efficiency to decrease from 71 % (control) to 32 % and 36 %, respectively. Biomass productivity also decreased from 11.5 mg TSS L<sup>−1</sup> d<sup>−1</sup> to 9.6 mg TSS L<sup>−1</sup> d<sup>−1</sup>. However, N-NH<sub>4</sub> removal remained high at 88 %, suggesting the functional resilience of ammonia-oxidizing bacteria. Additionally, a predictive metabolic pathway for bacteria was investigated, indicating prevailing ammonia oxidation and dehalogenation. <em>Tetradesmus obliquus</em> was the dominant microalga (>95 %) in the photobioreactors. Although no significant difference was observed in the composition of microalgae during the experiments with and without micropollutants, the presence of the 12 selected micropollutants may have inhibited microalgal metabolism during treatment. In relation to bacteria, Patescibacteria seems to be an indicator of the presence of micropollutants.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"93 ","pages":"Article 104496"},"PeriodicalIF":4.5,"publicationDate":"2025-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145836702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the role of Sargassum spp. polysaccharides in the development of innovative drug delivery systems 探索马尾藻多糖在创新给药系统开发中的作用

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2025-12-20 DOI: 10.1016/j.algal.2025.104492

Kit-Leong Cheong , Wenjie Chen , Min Wang , Saiyi Zhong , Malairaj Sathuvan

The increasing demand for sustainable and effective drug delivery systems has spurred significant interest in marine-derived polysaccharides, particularly those extracted from Sargassum species. This review explores the unique chemical structures and properties of Sargassum spp. polysaccharides, including fucoidan, alginate, and laminaran, and evaluates their potential for enhancing drug delivery. This review discusses various design and synthesis strategies for polysaccharide-based drug delivery systems such as nanoparticles, nanospheres, hydrogels, micelles, vesicles, and films. These drug delivery systems offer improved stability, controlled release, and targeted delivery, making them ideal for therapeutic applications. The key mechanisms underlying effective drug delivery, including encapsulation efficiency, biodegradability, release control, and pH sensitivity, are also explored. These mechanisms elucidate how Sargassum polysaccharides facilitate sustained and efficient delivery of therapeutic agents. In addition, this review covers multiple routes of administration (oral, mucosal, intravenous, intraperitoneal, and transdermal), highlighting the specific challenges and advantages of each method when using Sargassum polysaccharides in clinical settings. In conclusion, Sargassum spp. polysaccharides present promising platforms for the development of advanced drug delivery systems. Their unique properties, including biodegradability, low toxicity, and ability to be tailored for controlled and targeted drug release, make them attractive options for drug delivery therapies.

对可持续和有效的药物输送系统日益增长的需求激发了人们对海洋来源的多糖的极大兴趣，特别是从马尾藻中提取的多糖。本文综述了马尾藻多糖的独特化学结构和性质，包括岩藻聚糖、海藻酸盐和层藻聚糖，并评价了它们增强药物传递的潜力。本文综述了基于多糖的药物传递系统的各种设计和合成策略，如纳米颗粒、纳米球、水凝胶、胶束、囊泡和薄膜。这些给药系统提供了更好的稳定性，控制释放和靶向给药，使其成为治疗应用的理想选择。本文还探讨了有效药物递送的关键机制，包括包封效率、生物降解性、释放控制和pH敏感性。这些机制阐明了马尾藻多糖如何促进治疗剂的持续和有效的递送。此外，本综述涵盖了多种给药途径（口服，粘膜，静脉注射，腹腔注射和透皮），强调了在临床使用马尾藻多糖时每种方法的具体挑战和优势。综上所述，马尾藻多糖为开发先进的给药系统提供了有前途的平台。它们独特的特性，包括可生物降解性，低毒性，以及为控制和靶向药物释放量身定制的能力，使它们成为药物输送治疗的有吸引力的选择。

{"title":"Exploring the role of Sargassum spp. polysaccharides in the development of innovative drug delivery systems","authors":"Kit-Leong Cheong , Wenjie Chen , Min Wang , Saiyi Zhong , Malairaj Sathuvan","doi":"10.1016/j.algal.2025.104492","DOIUrl":"10.1016/j.algal.2025.104492","url":null,"abstract":"<div><div>The increasing demand for sustainable and effective drug delivery systems has spurred significant interest in marine-derived polysaccharides, particularly those extracted from <em>Sargassum</em> species. This review explores the unique chemical structures and properties of <em>Sargassum</em> spp. polysaccharides, including fucoidan, alginate, and laminaran, and evaluates their potential for enhancing drug delivery. This review discusses various design and synthesis strategies for polysaccharide-based drug delivery systems such as nanoparticles, nanospheres, hydrogels, micelles, vesicles, and films. These drug delivery systems offer improved stability, controlled release, and targeted delivery, making them ideal for therapeutic applications. The key mechanisms underlying effective drug delivery, including encapsulation efficiency, biodegradability, release control, and pH sensitivity, are also explored. These mechanisms elucidate how <em>Sargassum</em> polysaccharides facilitate sustained and efficient delivery of therapeutic agents. In addition, this review covers multiple routes of administration (oral, mucosal, intravenous, intraperitoneal, and transdermal), highlighting the specific challenges and advantages of each method when using <em>Sargassum</em> polysaccharides in clinical settings. In conclusion, <em>Sargassum</em> spp. polysaccharides present promising platforms for the development of advanced drug delivery systems. Their unique properties, including biodegradability, low toxicity, and ability to be tailored for controlled and targeted drug release, make them attractive options for drug delivery therapies.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"93 ","pages":"Article 104492"},"PeriodicalIF":4.5,"publicationDate":"2025-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145836700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microalgae-derived biodiesel: Review on microalgae species, genetic modification, cultivation strategies, and mutagenesis 微藻衍生生物柴油：微藻种类、基因改造、培养策略和诱变研究进展

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2025-12-19 DOI: 10.1016/j.algal.2025.104490

Jing Tian , Jianqun Lin , Guoqiang Zhuang , Peiyao Sun , Pengxue Geng , Linxu Chen , Xianke Chen

The growing global demand for unconventional energy sources, particularly biofuels, is driven by fossil fuel depletion, climate change, energy security concerns, and policy incentives. Microalgae, as a third-generation biofuel feedstock, are an ideal candidate for biodiesel production due to their high CO₂ fixation capacity, high productivity, environmental sustainability, and favorable biodiesel properties. This review systematically evaluates diverse microalgal species, their lipid profiles, and fatty acid compositions to identify optimal strains for biodiesel synthesis. From a cultivation perspective, we examined the effects of key environmental parameters (such as temperature, irradiance, and CO₂ concentrations) on microalgal growth and lipid biosynthesis, with particular emphasis on the mechanisms regulating lipid productivity and fatty acid composition. Furthermore, building upon current knowledge of algal lipid metabolism, we discuss advanced genetic engineering strategies for targeted metabolic pathway modification to develop high-lipid mutants or transgenic strains. While genetic manipulation enables precise metabolic control, its widespread application is limited by technical complexity, high costs, and insufficient genomic information, especially for extremophilic species. Therefore, this review analyzes current achievements and challenges in microalgal genetic engineering while highlighting the advantages and future potential of random mutagenesis for developing novel algal strains for biodiesel production.

化石燃料枯竭、气候变化、能源安全问题和政策激励等因素推动了全球对非常规能源（尤其是生物燃料）日益增长的需求。微藻作为第三代生物燃料原料，因其CO2固定能力强、生产效率高、环境可持续性好、生物柴油性能好等优点，成为生产生物柴油的理想选择。本综述系统地评价了不同的微藻种类、它们的脂质谱和脂肪酸组成，以确定合成生物柴油的最佳菌株。从培养的角度来看，我们研究了关键环境参数（如温度、辐照度和CO2浓度）对微藻生长和脂质生物合成的影响，特别强调了调节脂质生产力和脂肪酸组成的机制。此外，基于目前藻类脂质代谢的知识，我们讨论了靶向代谢途径修饰的先进基因工程策略，以开发高脂突变体或转基因菌株。虽然基因操作可以实现精确的代谢控制，但其广泛应用受到技术复杂性，高成本和基因组信息不足的限制，特别是对于极端微生物物种。因此，本文分析了目前微藻基因工程的成就和面临的挑战，同时强调了随机诱变技术在开发生物柴油用新型藻类菌株方面的优势和未来潜力。

{"title":"Microalgae-derived biodiesel: Review on microalgae species, genetic modification, cultivation strategies, and mutagenesis","authors":"Jing Tian , Jianqun Lin , Guoqiang Zhuang , Peiyao Sun , Pengxue Geng , Linxu Chen , Xianke Chen","doi":"10.1016/j.algal.2025.104490","DOIUrl":"10.1016/j.algal.2025.104490","url":null,"abstract":"<div><div>The growing global demand for unconventional energy sources, particularly biofuels, is driven by fossil fuel depletion, climate change, energy security concerns, and policy incentives. Microalgae, as a third-generation biofuel feedstock, are an ideal candidate for biodiesel production due to their high CO<sub>2</sub> fixation capacity, high productivity, environmental sustainability, and favorable biodiesel properties. This review systematically evaluates diverse microalgal species, their lipid profiles, and fatty acid compositions to identify optimal strains for biodiesel synthesis. From a cultivation perspective, we examined the effects of key environmental parameters (such as temperature, irradiance, and CO<sub>2</sub> concentrations) on microalgal growth and lipid biosynthesis, with particular emphasis on the mechanisms regulating lipid productivity and fatty acid composition. Furthermore, building upon current knowledge of algal lipid metabolism, we discuss advanced genetic engineering strategies for targeted metabolic pathway modification to develop high-lipid mutants or transgenic strains. While genetic manipulation enables precise metabolic control, its widespread application is limited by technical complexity, high costs, and insufficient genomic information, especially for extremophilic species. Therefore, this review analyzes current achievements and challenges in microalgal genetic engineering while highlighting the advantages and future potential of random mutagenesis for developing novel algal strains for biodiesel production.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"93 ","pages":"Article 104490"},"PeriodicalIF":4.5,"publicationDate":"2025-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145836699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potential of pyrolysis products of Enzyme-Treated vs. Intact Nannochloropsis gaditana: physicochemical characterization and antifungal activity 酶处理的纳米绿草藻热解产物与完整的纳米绿草藻热解产物的潜力：理化特性和抗真菌活性

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2025-12-16 DOI: 10.1016/j.algal.2025.104482

Wessal Ouedrhiri , Abderrahman Mellalou , Zouhair Ait Taleb , Abderrahim Aasfar , Bader Flissate , Mariam Tounsi , Abdelkader Outzourhit , Hicham El-Arroussi , Fouad Ghamouss

This study examines the pyrolysis products derived from two types of Nannochloropsis gaditana biomass: untreated intact biomass (IntBA) and enzymatically pretreated biomass (EnzBA), with the latter regarded as a byproduct of the enzymatic extraction process. The analysis aims to evaluate how enzymatic pretreatment influences the yield and composition of the pyrolysis-derived products, providing insights into the valorization potential of enzymatic extraction residues. Comprehensive characterization of biochar and bio-oil derived from pyrolysis at 600 °C was conducted, highlighting the influence of the feedstock composition on product yields and quality. Bio-oil and biochar yields for IntBA were 51.7 % and 26.9 %, respectively, whereas EnzBA produced a higher biochar yield (55.3 %) but lower bio-oil yield (24.6 %). Physicochemical analyses, including FTIR, GC–MS, and TGA, revealed significant differences in the structural and chemical properties of the products. Notably, the biochar produced from IntBA exhibited superior thermal stability and a higher carbon content (65 %) compared to the biochar derived from EnzBA (49 %). The IntBA biochar also showed a BET surface area of 249 m²/g and a total pore volume of 0.39 cm³/g, whereas the EnzBA biochar displayed no pore development, with no measurable BET surface area and no observable porosity in the SEM images. Moreover, the IntBA biochar presented a high degree of structural disorder, evidenced by an ID/IG ratio of 0.94. In contrast, the bio-oils contained a variety of oxygenated and nitrogenous compounds suitable for biofuel applications. The IntBA and EnzBA bio-oils exhibited flash points of 130 °C and 135 °C and water contents of 21.3 % and 33.7 %, respectively. Additionally, antifungal assays revealed promising bioactivity, with pyrolyzed oils inhibiting fungal growth against Botrytis cinerea, Fusarium oxysporum, and Fusarium solani, showcasing potential in agricultural applications. This research underscores the dual benefits of enzymatic pretreatment and pyrolysis in valorizing microalgae biomass byproducts, offering sustainable avenues for bioenergy and bioproducts development.

本研究考察了两种类型的纳米绿opsis gaditana生物质的热解产物：未经处理的完整生物质（IntBA）和酶预处理的生物质（EnzBA），后者被认为是酶提取过程的副产品。该分析旨在评估酶预处理如何影响热解衍生产品的产量和组成，为酶提取残留物的增值潜力提供见解。对600°C热解所得的生物炭和生物油进行了综合表征，重点研究了原料组成对产品收率和质量的影响。IntBA的生物油和生物炭产率分别为51.7%和26.9%，而EnzBA的生物炭产率较高（55.3%），生物油产率较低（24.6%）。理化分析，包括FTIR， GC-MS和TGA，揭示了产品的结构和化学性质的显著差异。值得注意的是，与由EnzBA衍生的生物炭（49%）相比，由IntBA生产的生物炭具有更好的热稳定性和更高的碳含量（65%）。IntBA生物炭的BET表面积为249 m2/g，总孔隙体积为0.39 cm3/g，而EnzBA生物炭没有孔隙发育，没有可测量的BET表面积，也没有在SEM图像中观察到孔隙度。此外，IntBA生物炭具有高度的结构无序性，其ID/IG比为0.94。相比之下，生物油含有各种适合生物燃料应用的含氧和含氮化合物。IntBA和EnzBA生物油的闪点分别为130℃和135℃，含水量分别为21.3%和33.7%。此外，抗真菌实验显示，热解油具有良好的生物活性，可以抑制葡萄枯萎菌、尖孢镰刀菌和茄枯菌的生长，具有潜在的农业应用潜力。本研究强调了酶预处理和热解对微藻生物质副产品的双重效益，为生物能源和生物产品的开发提供了可持续的途径。

{"title":"Potential of pyrolysis products of Enzyme-Treated vs. Intact Nannochloropsis gaditana: physicochemical characterization and antifungal activity","authors":"Wessal Ouedrhiri , Abderrahman Mellalou , Zouhair Ait Taleb , Abderrahim Aasfar , Bader Flissate , Mariam Tounsi , Abdelkader Outzourhit , Hicham El-Arroussi , Fouad Ghamouss","doi":"10.1016/j.algal.2025.104482","DOIUrl":"10.1016/j.algal.2025.104482","url":null,"abstract":"<div><div>This study examines the pyrolysis products derived from two types of <em>Nannochloropsis gaditana</em> biomass: untreated intact biomass (IntBA) and enzymatically pretreated biomass (EnzBA), with the latter regarded as a byproduct of the enzymatic extraction process. The analysis aims to evaluate how enzymatic pretreatment influences the yield and composition of the pyrolysis-derived products, providing insights into the valorization potential of enzymatic extraction residues. Comprehensive characterization of biochar and bio-oil derived from pyrolysis at 600 °C was conducted, highlighting the influence of the feedstock composition on product yields and quality. Bio-oil and biochar yields for IntBA were 51.7 % and 26.9 %, respectively, whereas EnzBA produced a higher biochar yield (55.3 %) but lower bio-oil yield (24.6 %). Physicochemical analyses, including FTIR, GC–MS, and TGA, revealed significant differences in the structural and chemical properties of the products. Notably, the biochar produced from IntBA exhibited superior thermal stability and a higher carbon content (65 %) compared to the biochar derived from EnzBA (49 %). The IntBA biochar also showed a BET surface area of 249 m<sup>2</sup>/g and a total pore volume of 0.39 cm<sup>3</sup>/g, whereas the EnzBA biochar displayed no pore development, with no measurable BET surface area and no observable porosity in the SEM images. Moreover, the IntBA biochar presented a high degree of structural disorder, evidenced by an ID/IG ratio of 0.94. In contrast, the bio-oils contained a variety of oxygenated and nitrogenous compounds suitable for biofuel applications. The IntBA and EnzBA bio-oils exhibited flash points of 130 °C and 135 °C and water contents of 21.3 % and 33.7 %, respectively. Additionally, antifungal assays revealed promising bioactivity, with pyrolyzed oils inhibiting fungal growth against <em>Botrytis cinerea</em>, <em>Fusarium oxysporum</em>, and <em>Fusarium solani</em>, showcasing potential in agricultural applications. This research underscores the dual benefits of enzymatic pretreatment and pyrolysis in valorizing microalgae biomass byproducts, offering sustainable avenues for bioenergy and bioproducts development.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"93 ","pages":"Article 104482"},"PeriodicalIF":4.5,"publicationDate":"2025-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145786529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and functional prediction of the C3H transcription factor family in Phaeodactylum tricornutum: Regulation of laminarin synthesis via circadian rhythm antagonistic interaction between PtC3H-1 and PtC3H-5 三角褐指藻C3H转录因子家族的鉴定和功能预测：通过PtC3H-1和PtC3H-5的昼夜节律拮抗相互作用调节层粘连蛋白合成

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2025-12-16 DOI: 10.1016/j.algal.2025.104485

Kanglie Guo , Zhitao Ye , Xiaomin Wu , Ruixin Li , Zhen Wang , Shuqi Wang , Douglas R. Tocher , Xiaojuan Liu

The CCCH-structured transcription factors (C3H) play critical roles in growth, development, and stress responses in higher plants, yet research on the C3H gene family in microalgae remains unreported. In this study, 10 PtC3H members were identified from the marine diatom P. tricornutum through HMM search. Bioinformatic analyses predicted that members of this family play a pivotal role in RNA binding-mediated post-transcriptional regulation. Three distinct expression patterns were identified for PtC3Hs under a 12-hour light/dark cycle. Notably, the expression profile of PtC3H-1 and PtC3H-5 suggests that they may regulate the biosynthesis of laminarin through circadian rhythm. Further analysis employing three-dimensional structural modeling and molecular docking predicted that PtC3H-1 may function through a dual mechanism: beyond RNA binding, it potentially activates the expression of downstream genes by interacting with cis-acting elements, thereby promoting laminarin synthesis. Concomitantly, the study found that PtC3H-5 regulate laminarin metabolism through its interaction with PtC3H-1. Based on the above, this study not only accomplished systematic identification and functional prediction of all PtC3Hs in P. tricornutum, enriching the theoretical basis of this family in diatom, but also predicted that PtC3H-1 and PtC3H-5 synergistically mediate circadian rhythm to dynamically regulate laminarin metabolic homeostasis, along with their core molecular mechanisms.

ccch结构的转录因子（C3H）在高等植物的生长发育和逆境响应中起着至关重要的作用，但微藻中C3H基因家族的研究尚未见报道。在本研究中，通过HMM搜索从海洋硅藻P. tricornutum中鉴定出10个PtC3H成员。生物信息学分析预测，该家族的成员在RNA结合介导的转录后调控中发挥关键作用。在12小时光照/黑暗循环下，PtC3Hs有三种不同的表达模式。值得注意的是，PtC3H-1和PtC3H-5的表达谱表明它们可能通过昼夜节律调节层粘胶蛋白的生物合成。通过三维结构建模和分子对接进一步分析，预测PtC3H-1可能通过双重机制发挥作用：除了RNA结合外，它还可能通过与顺式作用元件相互作用激活下游基因的表达，从而促进层粘连蛋白的合成。同时，研究发现PtC3H-5通过与PtC3H-1的相互作用调节层粘连蛋白的代谢。在此基础上，本研究不仅完成了对三角藻中所有PtC3Hs的系统鉴定和功能预测，丰富了该家族在硅藻中的理论基础，还预测了PtC3H-1和PtC3H-5协同调节昼夜节律，动态调节层粘连蛋白代谢稳态及其核心分子机制。

{"title":"Identification and functional prediction of the C3H transcription factor family in Phaeodactylum tricornutum: Regulation of laminarin synthesis via circadian rhythm antagonistic interaction between PtC3H-1 and PtC3H-5","authors":"Kanglie Guo , Zhitao Ye , Xiaomin Wu , Ruixin Li , Zhen Wang , Shuqi Wang , Douglas R. Tocher , Xiaojuan Liu","doi":"10.1016/j.algal.2025.104485","DOIUrl":"10.1016/j.algal.2025.104485","url":null,"abstract":"<div><div>The CCCH-structured transcription factors (C3H) play critical roles in growth, development, and stress responses in higher plants, yet research on the <em>C3H</em> gene family in microalgae remains unreported. In this study, 10 <em>PtC3H</em> members were identified from the marine diatom <em>P. tricornutum</em> through HMM search. Bioinformatic analyses predicted that members of this family play a pivotal role in RNA binding-mediated post-transcriptional regulation. Three distinct expression patterns were identified for <em>PtC3Hs</em> under a 12-hour light/dark cycle. Notably, the expression profile of <em>PtC3H-1</em> and <em>PtC3H-5</em> suggests that they may regulate the biosynthesis of laminarin through circadian rhythm. Further analysis employing three-dimensional structural modeling and molecular docking predicted that <em>PtC3H-1</em> may function through a dual mechanism: beyond RNA binding, it potentially activates the expression of downstream genes by interacting with <em>cis</em>-acting elements, thereby promoting laminarin synthesis. Concomitantly, the study found that <em>PtC3H-5</em> regulate laminarin metabolism through its interaction with <em>PtC3H-1</em>. Based on the above, this study not only accomplished systematic identification and functional prediction of all PtC3Hs in <em>P. tricornutum</em>, enriching the theoretical basis of this family in diatom, but also predicted that <em>PtC3H-1</em> and <em>PtC3H-5</em> synergistically mediate circadian rhythm to dynamically regulate laminarin metabolic homeostasis, along with their core molecular mechanisms.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"93 ","pages":"Article 104485"},"PeriodicalIF":4.5,"publicationDate":"2025-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145836602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0