Algal Research-Biomass Biofuels and Bioproducts最新文献_第4页

Neocystis mucosa, a polar alga producing oils containing polyunsaturated essential fatty acids

IF 4.6 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2025-01-29 DOI: 10.1016/j.algal.2025.103939

T. Řezanka , J. Elster , D. Kubáč , J. Kvíderová , L. Procházková , M. Vítová , J. Lukavský

The sustainable cultivation of microalgae for bioactive compounds, such as polyunsaturated fatty acids (PUFAs), is vital for addressing global health and nutritional challenges. However, the availability of algal strains suitable for year-round cultivation in temperate climates is limited. This study investigates the psychrotolerant green alga Neocystis mucosa (strain Šnokhousová and Elster, CCALA No. 1141), isolated from Svalbard soil, as a promising candidate for outdoor cultivation in Mid-European climates.

Molecular analysis confirmed the identification of the strain as N. mucosa. It demonstrated optimal growth at 12 °C under low light intensity (10 μmol·m⁻²·s⁻¹). However, it tolerates short-term light and temperature fluctuations (5 °C to 25 °C). This resilience ensures that there is no significant risk of cell death due to temperature and light variations, such as those that occur during sunny winter days. Nighttime LED lighting increased growth by 160 % compared to sunlight alone, yielding biomass production rates of 1.73 g·m⁻²·d⁻¹ and 0.135 g·L⁻¹·d⁻¹, with a maximum dry matter of 6.3 g·L⁻¹. The strain displayed high lipid productivity, with unsaturated fatty acids comprising 51–63 % of total fatty acid content. Notable components included linoleic acid (14.9–17.1 %), α-linolenic acid (18.3–23.1 %), and hexadecatetraenoic acid (10.2–16.7 %). Lipid and PUFA percentages increased with culture age.

This study highlights the practical potential of polar N. mucosa for year-round cultivation in temperate climates, offering a sustainable source of PUFAs for food supplementation and immunomodulation, including reducing risks associated with COVID-19. These findings advance biotechnological approaches to sustainable PUFA production, addressing global dietary needs.

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引用次数: 0

Impact of water quality parameters on harmful algal bloom mitigation and phosphorus removal by lab-synthesized γFe2O3/TiO2 magnetic photocatalysts

IF 4.6 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2025-01-27 DOI: 10.1016/j.algal.2025.103932

Nafeesa Khan , Partha Protim Bhowmik , Md Sayeduzzaman Sarker , Haoran Yang , Ruopu Li , Jia Liu

Harmful algal blooms (HABs) are a growing problem in freshwater systems, posing risks to human health and ecosystems. This study explores the use of γFe₂O₃/TiO₂ nanocomposites for HAB mitigation and phosphorus removal in lake water. The nanocomposite showed strong photocatalytic effects under simulated sunlight, having a Fe to Ti ratio of 2.2:1 and a specific surface area of 116.52 m²/g. It achieved 95 % inactivation of Microcystis aeruginosa and 90 % inactivation of Cylindrospermopsis raciborskii within 1 h at 100 mg/L concentration. In lake water, the inactivation of M. aeruginosa was reduced to 88 % due to the complex environment. Phosphorus adsorption was also effective, with 35 % removal in deionized water and 23 % in lake water. Environmental factors such as temperature, pH, natural organic matter (NOM), and alkalinity impact both processes. The inactivation of C. raciborskii increased at higher temperatures, while M. aeruginosa showed better inactivation at lower temperatures. The nanocomposite was most effective for M. aeruginosa inactivation at pH 7.5 and achieved the maximum phosphorus removal at 24 °C and pH 7.5. While NOM slightly inhibited performance, alkalinity significantly reduced both inactivation and adsorption. Phosphorus desorption tests demonstrated a 64 % recovery rate, indicating the potential for reusing the nanocomposite. Despite challenges in complex environments, the nanocomposite shows great potential for HAB mitigation and phosphorus removal in freshwater systems.

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引用次数: 0

Effect of nitrogen limitation and irradiance on the biochemical composition of Haslea ostrearia

IF 4.6 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2025-01-27 DOI: 10.1016/j.algal.2025.103931

Hillary Patricia Brenes-Monge, M. del Pilar Sánchez-Saavedra

The marine diatom Haslea ostrearia is a valuable resource for biotechnological applications. However, its biochemical responses to crucial culture conditions, such as nitrogen availability and light intensity, still need to be explored. Therefore, this study evaluates the effects of nitrogen limitation and irradiance on the biochemical profile of H. ostrearia. Cultures were grown in two media: standard “f” medium (control) or “f” medium with a tenfold reduction in sodium nitrate concentration (1.7 × 10⁻⁴ M). These cultures were exposed to low (50 μmol m⁻² s⁻¹) or high (200 μmol m⁻² s⁻¹) irradiance and analyzed during exponential and stationary growth phases. Nitrogen control conditions with high irradiance resulted in the highest proteins (41 % based on organic dry weight, ODW), carbohydrates (8 % based on ODW), and pigments content, as well as the essential fatty acids EPA (19 % of total identified fatty acids, TIFA) and DHA (3 % of TIFA). Notably, under nitrogen-limited conditions combined with high irradiance during the stationary growth phase significantly increased lipids content (25 % based on ODW) and the proportions of palmitic (36 % of TIFA) and palmitoleic (30 % of TIFA) fatty acids. These findings emphasize the importance of optimizing nitrogen and irradiance conditions to enhance metabolite production in H. ostrearia, underscoring its potential in aquaculture, biotechnology, and functional foods industries.

{"title":"Effect of nitrogen limitation and irradiance on the biochemical composition of Haslea ostrearia","authors":"Hillary Patricia Brenes-Monge, M. del Pilar Sánchez-Saavedra","doi":"10.1016/j.algal.2025.103931","DOIUrl":"10.1016/j.algal.2025.103931","url":null,"abstract":"<div><div>The marine diatom <em>Haslea ostrearia</em> is a valuable resource for biotechnological applications. However, its biochemical responses to crucial culture conditions, such as nitrogen availability and light intensity, still need to be explored. Therefore, this study evaluates the effects of nitrogen limitation and irradiance on the biochemical profile of <em>H. ostrearia.</em> Cultures were grown in two media: standard “f” medium (control) or “f” medium with a tenfold reduction in sodium nitrate concentration (1.7 × 10<sup>−4</sup> M). These cultures were exposed to low (50 μmol m<sup>−2</sup> s<sup>−1</sup>) or high (200 μmol m<sup>−2</sup> s<sup>−1</sup>) irradiance and analyzed during exponential and stationary growth phases. Nitrogen control conditions with high irradiance resulted in the highest proteins (41 % based on organic dry weight, ODW), carbohydrates (8 % based on ODW), and pigments content, as well as the essential fatty acids EPA (19 % of total identified fatty acids, TIFA) and DHA (3 % of TIFA). Notably, under nitrogen-limited conditions combined with high irradiance during the stationary growth phase significantly increased lipids content (25 % based on ODW) and the proportions of palmitic (36 % of TIFA) and palmitoleic (30 % of TIFA) fatty acids. These findings emphasize the importance of optimizing nitrogen and irradiance conditions to enhance metabolite production in <em>H. ostrearia,</em> underscoring its potential in aquaculture, biotechnology, and functional foods industries.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"86 ","pages":"Article 103931"},"PeriodicalIF":4.6,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143144499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Removal of single and multi-heavy metals from piggery digestate by the electric field-microalgae system: Influences, kinetics and mechanisms

IF 4.6 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2025-01-25 DOI: 10.1016/j.algal.2025.103934

Xiaosong Tian , Min Wang , Xing Liao , Shiyu Chu , Haixiang Cheng , Xiaoai Lin , Longzao Luo

Electric field-microalgae system (EFMS) has demonstrated significant potential in removing humic acids and nutrients from the piggery digestate, while its removal of heavy metals (HMs) has not been extensively studied. This study investigated the factors influencing the removal of HMs removal by EFMS, as well as the kinetics and underlying mechanisms. Results showed that the removal of single HM by EFMS was dependent on the initial concentration of HM, with the highest removal efficiency being 85.54 % for Cu²⁺ at 4.00 mg/L, 97.87 % for Zn²⁺ at 10.00 mg/L, and 99.07 % for Cd²⁺ at 2.00 mg/L, respectively. Cu²⁺ was removed preferentially by EFMS when the three HMs coexisted, achieving an efficiency of approximately 86 % within 1 h. Meanwhile, the removal efficiency of Zn²⁺ and Cd²⁺ approached 85 % after 1.5 h. The simultaneous removal of multiple HMs by EFMS was affected by factors including the intensity of the electric field, the concentration of microalgae inoculum, and the pH level. The removal of the three HMs by EFMS followed the pseudo-first-order kinetic model. The primary mechanism of HMs removal by EFMS involved oxidation reactions induced by the electric field, followed by adsorption processes.

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引用次数: 0

Metabolome analysis reveals the involvement of oxylipins in regulating the maturation of conchosporangia in Pyropia haitanensis

IF 4.6 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2025-01-25 DOI: 10.1016/j.algal.2025.103933

Yi Liu , Haike Qian , Shanshan Zhu , Tingting Niu , Qijun Luo , Juanjuan Chen , Rui Yang , Peng Zhang , Tiegan Wang , Haimin Chen

In Porphyra sensu lato, the development of the conchocelis into conchosporangia depends on a suite of environmental factors. This process is not entirely controllable, a situation that to some extent has limited the development of the seaweed industry. To understand the mechanisms underlying this process, changes in the cellular morphology of Pyropia haitanensis were recorded, and the structural differences between conchocelis and conchosporangia were observed using transmission electron microscopy. Metabolome analysis was employed to compare the metabolite profiles throughout the development of the conchosporangia. The results showed significant differences in cell morphology between conchocelis and conchosporangia, with conchosporangia cells exhibiting increased diameters due to apical swelling and growth. Within the cells, the number of vacuoles and the amount of floridean starch were increased, while the cell walls and mucilage layers became thickened. Metabolome analysis indicated that the lipoxygenase (LOX) pathway was likely involved in the formation of conchosporangia, with oxylipins derived from C18 and C20 being abundant during maturation. Oxylipins such as oxo-eicosatetraenoic acid (KETE), methyl jasmonic acid (MeJA), and prostaglandins were significantly increased during maturation. PhLOX genes related to oxylipin synthesis showed differential expression throughout the maturation process. Additionally, the exogenous application of MeJA and 5-KETE enhanced the formation of conchosporangia. Overall, the results suggest that the LOX pathway may play a key role in regulating the maturation of conchosporangia in Porphyra sensu lato. and that oxylipins could potentially be used as industrial seedling regulators.

{"title":"Metabolome analysis reveals the involvement of oxylipins in regulating the maturation of conchosporangia in Pyropia haitanensis","authors":"Yi Liu , Haike Qian , Shanshan Zhu , Tingting Niu , Qijun Luo , Juanjuan Chen , Rui Yang , Peng Zhang , Tiegan Wang , Haimin Chen","doi":"10.1016/j.algal.2025.103933","DOIUrl":"10.1016/j.algal.2025.103933","url":null,"abstract":"<div><div>In <em>Porphyra</em> sensu lato, the development of the conchocelis into conchosporangia depends on a suite of environmental factors. This process is not entirely controllable, a situation that to some extent has limited the development of the seaweed industry. To understand the mechanisms underlying this process, changes in the cellular morphology of <em>Pyropia haitanensis</em> were recorded, and the structural differences between conchocelis and conchosporangia were observed using transmission electron microscopy. Metabolome analysis was employed to compare the metabolite profiles throughout the development of the conchosporangia. The results showed significant differences in cell morphology between conchocelis and conchosporangia, with conchosporangia cells exhibiting increased diameters due to apical swelling and growth. Within the cells, the number of vacuoles and the amount of floridean starch were increased, while the cell walls and mucilage layers became thickened. Metabolome analysis indicated that the lipoxygenase (LOX) pathway was likely involved in the formation of conchosporangia, with oxylipins derived from C18 and C20 being abundant during maturation. Oxylipins such as oxo-eicosatetraenoic acid (KETE), methyl jasmonic acid (MeJA), and prostaglandins were significantly increased during maturation. PhLOX genes related to oxylipin synthesis showed differential expression throughout the maturation process. Additionally, the exogenous application of MeJA and 5-KETE enhanced the formation of conchosporangia. Overall, the results suggest that the LOX pathway may play a key role in regulating the maturation of conchosporangia in <em>Porphyra</em> sensu lato. and that oxylipins could potentially be used as industrial seedling regulators.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"86 ","pages":"Article 103933"},"PeriodicalIF":4.6,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143144575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Digital holography unveils sub-lethal copper doses using motility patterns of Tetraselmis microalgae bioprobes

IF 4.6 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2025-01-22 DOI: 10.1016/j.algal.2025.103928

Giusy Giugliano , Marika Valentino , Elena Cavalletti , Pasquale Memmolo , Lisa Miccio , Vittorio Bianco , Angela Sardo , Pietro Ferraro

The escalating issue of marine pollution stemming from heavy metals, notably copper, demands urgent attention. To effectively address this concern, the development of reliable techniques is critical to accurately assess the impact of contamination on aquatic ecosystems. Tetraselmis, a genus of unicellular and motile green algae, contributes significantly to the regulation of aquatic ecosystems. However, Tetraselmis vital processes and cellular functions may be altered by the presence of copper in marine environments. Its sensitivity to copper pollution allows exploiting Tetraselmis as a bioindicator of toxicity in waters. In fact, elevated concentrations of copper (exceeding 10 μmol) cause Tetraselmis death, a process that can be easily detected using any imaging technique, as the dead microalgae become motionless. In contrast, at lower copper concentrations, this effect is not observable. Here, we demonstrate that Tetraselmis 3D motility is correlated with copper pollution level in water. We leverage refocusing capability and 3D tracking of Digital Holography (DH) to monitor Tetraselmis trajectories and their morphological parameters. We use morphometric and kinematic information to distinguish between different copper doses over various exposure times. We perform both binary and multiclass classifications to analyze the data. In the binary approach, we achieved an accuracy of over 92 % in differentiating Tetraselmis exposed to copper from those unexposed. In the multiclass approach, we successfully identified and classified different sublethal copper doses, achieving an accuracy of over 84 %. These results show that both Tetraselmis motility and morphology can serve as an effective bioindicator for assessing copper pollution in water samples.

{"title":"Digital holography unveils sub-lethal copper doses using motility patterns of Tetraselmis microalgae bioprobes","authors":"Giusy Giugliano , Marika Valentino , Elena Cavalletti , Pasquale Memmolo , Lisa Miccio , Vittorio Bianco , Angela Sardo , Pietro Ferraro","doi":"10.1016/j.algal.2025.103928","DOIUrl":"10.1016/j.algal.2025.103928","url":null,"abstract":"<div><div>The escalating issue of marine pollution stemming from heavy metals, notably copper, demands urgent attention. To effectively address this concern, the development of reliable techniques is critical to accurately assess the impact of contamination on aquatic ecosystems. <em>Tetraselmis</em>, a genus of unicellular and motile green algae, contributes significantly to the regulation of aquatic ecosystems. However, <em>Tetraselmis</em> vital processes and cellular functions may be altered by the presence of copper in marine environments. Its sensitivity to copper pollution allows exploiting <em>Tetraselmis</em> as a bioindicator of toxicity in waters. In fact, elevated concentrations of copper (exceeding 10 μmol) cause <em>Tetraselmis</em> death, a process that can be easily detected using any imaging technique, as the dead microalgae become motionless. In contrast, at lower copper concentrations, this effect is not observable. Here, we demonstrate that <em>Tetraselmis</em> 3D motility is correlated with copper pollution level in water. We leverage refocusing capability and 3D tracking of Digital Holography (DH) to monitor <em>Tetraselmis</em> trajectories and their morphological parameters. We use morphometric and kinematic information to distinguish between different copper doses over various exposure times. We perform both binary and multiclass classifications to analyze the data. In the binary approach, we achieved an accuracy of over 92 % in differentiating <em>Tetraselmis</em> exposed to copper from those unexposed. In the multiclass approach, we successfully identified and classified different sublethal copper doses, achieving an accuracy of over 84 %. These results show that both <em>Tetraselmis</em> motility and morphology can serve as an effective bioindicator for assessing copper pollution in water samples.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"86 ","pages":"Article 103928"},"PeriodicalIF":4.6,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143144578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mycosporine-like amino acids profile in red algae from high UV-index geographical areas (San Andrés Island and La Guajira) of the Colombian Caribbean coast

IF 4.6 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2025-01-22 DOI: 10.1016/j.algal.2025.103927

Vanessa Urrea-Victoria , Geison Costa , Brigitte Gavio , Freddy Ramos , Leonardo Castellanos

Mycosporine-like amino acids (MAAs) are photoprotective compounds with diverse applications in biotechnology, pharmaceuticals, and cosmetics. This study aimed to identify and quantify the full range of MAAs present in red macroalgae collected from high UV-Index geographical areas (San Andrés Island and La Guajira) of the Colombian Caribbean coast. We focused on abundant species from the midlittoral zone, covering contrasting UV-index periods throughout 2022. Quantification was performed using the validated calibration curve of porphyra-334, corrected with the molar extinction coefficients for shinorine, palythine, and asterine, considering the area under the curve at their maximum absorption wavelengths for accurate MAA concentration estimates. Total MAAs content was obtained from different species of the orders Ceramiales, Gigartinales, Gracilariales, Halymeniales, and Nemiales collected from the Colombian Caribbean coast. Several species from this region, characterized by its high UV-Index, exhibited higher MAAs concentrations compared to those reported in other tropical regions. The highest total MAAs content was found in Bryocladia cuspidata with 15.93 ± 0.02 mg.g^-1 DW. However, other species showed significant potential, with values above 6 mg.g^-1 DW such as Centroceras micracanthum, and Laurencia obtusa. Other species had values around 3 mg.g^-1 DW, such as Hypnea cf. caraibica, Gracilaria tikvahiae, and Grateloupia cf. filicina. Some species are viable for sustainable MAAs extraction, highlighting the relevance of Colombian red macroalgae as sources for photoprotective applications.

{"title":"Mycosporine-like amino acids profile in red algae from high UV-index geographical areas (San Andrés Island and La Guajira) of the Colombian Caribbean coast","authors":"Vanessa Urrea-Victoria , Geison Costa , Brigitte Gavio , Freddy Ramos , Leonardo Castellanos","doi":"10.1016/j.algal.2025.103927","DOIUrl":"10.1016/j.algal.2025.103927","url":null,"abstract":"<div><div>Mycosporine-like amino acids (MAAs) are photoprotective compounds with diverse applications in biotechnology, pharmaceuticals, and cosmetics. This study aimed to identify and quantify the full range of MAAs present in red macroalgae collected from high UV-Index geographical areas (San Andrés Island and La Guajira) of the Colombian Caribbean coast. We focused on abundant species from the midlittoral zone, covering contrasting UV-index periods throughout 2022. Quantification was performed using the validated calibration curve of porphyra-334, corrected with the molar extinction coefficients for shinorine, palythine, and asterine, considering the area under the curve at their maximum absorption wavelengths for accurate MAA concentration estimates. Total MAAs content was obtained from different species of the orders Ceramiales, Gigartinales, Gracilariales, Halymeniales, and Nemiales collected from the Colombian Caribbean coast. Several species from this region, characterized by its high UV-Index, exhibited higher MAAs concentrations compared to those reported in other tropical regions. The highest total MAAs content was found in <em>Bryocladia cuspidata</em> with 15.93 ± 0.02 mg.g<sup>-1</sup> DW. However, other species showed significant potential, with values above 6 mg.g<sup>-1</sup> DW such as <em>Centroceras micracanthum</em>, and <em>Laurencia obtusa</em>. Other species had values around 3 mg.g<sup>-1</sup> DW, such as <em>Hypnea</em> cf. <em>caraibica</em>, <em>Gracilaria tikvahiae</em>, and <em>Grateloupia</em> cf<em>. filicina</em>. Some species are viable for sustainable MAAs extraction, highlighting the relevance of Colombian red macroalgae as sources for photoprotective applications.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"86 ","pages":"Article 103927"},"PeriodicalIF":4.6,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143144505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immobilized Chlorella salina for removing nitrogenous and phosphorous compounds from mariculture tail water

IF 4.6 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2025-01-21 DOI: 10.1016/j.algal.2025.103930

Pengbing Pei , Zijie Xie , Muhammad Aslam , Shuangcheng Jiang , Qi Lin , Hongli Cui , Hong Du

The rapid development of the maricultural industry has brought both economic benefits and environmental challenges. One such challenge is the purification of mariculture tail water (MTW), which has become a key issue of marine environmental protection. Meanwhile, immobilized algal beads (IAB), has received growing attention as cost-effective wastewater treatment technology. However, most of the studies on the treatment of aquaculture tail water by IAB focus on the application of freshwater, and the use of seawater remains relatively rare. This study investigated the screening of algal species and embedding particle size suitable for the treatment of artificial MTWI, the optimization of algal beads preparation using the response surface method, and their application in treating artificial MTW-II. The results showed that immobilized Chlorella salina harbouring particle size of 3.2 mm were most effective for removing N from artificial MTWI. IAB fabricated under the optimized conditions of 4.2 % (w/v) sodium alginate (SA), 1 × 10⁷ cells·mL⁻¹ initial density of C. salina and 130 mL algal cells volume achieved the highest NH₄-N removal efficiency (75.780 %) during the 72 h experiment. Although immobilized cultivation inhibited algal cell photosynthesis, it also promoted their growth and NH₄-N removal ability. Groups with 10 % algal beads exhibited a better purification effect and could treat 5 batches of artificial MTW-II containing 815.75 mg of inorganic nitrogen and 71.80 mg of phosphate. Furthermore, the addition of 2 % algal beads was the most cost-effective scheme, resulting in a treatment cost of approximately 3.50 RMB per ton of tail water and 0.33 RMB per gram of inorganic nitrogen. These findings provide unique insight into marine environmental protection and suggest that IAB application offers a promising method for integrated MTW treatment.

{"title":"Immobilized Chlorella salina for removing nitrogenous and phosphorous compounds from mariculture tail water","authors":"Pengbing Pei , Zijie Xie , Muhammad Aslam , Shuangcheng Jiang , Qi Lin , Hongli Cui , Hong Du","doi":"10.1016/j.algal.2025.103930","DOIUrl":"10.1016/j.algal.2025.103930","url":null,"abstract":"<div><div>The rapid development of the maricultural industry has brought both economic benefits and environmental challenges. One such challenge is the purification of mariculture tail water (MTW), which has become a key issue of marine environmental protection. Meanwhile, immobilized algal beads (IAB), has received growing attention as cost-effective wastewater treatment technology. However, most of the studies on the treatment of aquaculture tail water by IAB focus on the application of freshwater, and the use of seawater remains relatively rare. This study investigated the screening of algal species and embedding particle size suitable for the treatment of artificial MTW<img>I, the optimization of algal beads preparation using the response surface method, and their application in treating artificial MTW-II. The results showed that immobilized <em>Chlorella salina</em> harbouring particle size of 3.2 mm were most effective for removing N from artificial MTW<img>I. IAB fabricated under the optimized conditions of 4.2 % (<em>w</em>/<em>v</em>) sodium alginate (SA), 1 × 10<sup>7</sup> cells·mL<sup>−1</sup> initial density of <em>C. salina</em> and 130 mL algal cells volume achieved the highest NH<sub>4</sub>-N removal efficiency (75.780 %) during the 72 h experiment. Although immobilized cultivation inhibited algal cell photosynthesis, it also promoted their growth and NH<sub>4</sub>-N removal ability. Groups with 10 % algal beads exhibited a better purification effect and could treat 5 batches of artificial MTW-II containing 815.75 mg of inorganic nitrogen and 71.80 mg of phosphate. Furthermore, the addition of 2 % algal beads was the most cost-effective scheme, resulting in a treatment cost of approximately 3.50 RMB per ton of tail water and 0.33 RMB per gram of inorganic nitrogen. These findings provide unique insight into marine environmental protection and suggest that IAB application offers a promising method for integrated MTW treatment.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"86 ","pages":"Article 103930"},"PeriodicalIF":4.6,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143436957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of wine lees ultrasonic cavitation as pre-treatment and its possible use as substrate for microalgae biomass production

IF 4.6 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2025-01-21 DOI: 10.1016/j.algal.2025.103929

Scarponi Paolina , Bravi Marco , Manente Sabrina , Cavinato Cristina

Ultrasonic cavitation is a technology used to improve organic carbon solubilization of a substrate, increasing its biodegradability for further biological treatment. Moreover, its application has a pasteurization effect on waste stream. In this study, different cavitation times (5, 10, 20 and 30 min) are tested on white and red wine lees to evaluate the organic compounds solubilization, physical-chemical variation and antimicrobial cavitation effects, with the aim of further valorization with biotechnological applications such as microalgae growth. Wine lees cavitated effluents are applied as substrate for Chlorella vulgaris growth, obtaining a microalgae-yeast microbial community biomass of 2.4 ± 0.7 g L⁻¹ (WL) and 1.7 ± 0.0 g L⁻¹ (RL) with 33.9 ± 4.1 % (WL) and 60.5 ± 5.3 % (RL) of lipids content. Ultrasound cavitation is efficient in controlling native microorganism proliferation (yeast and mold) only applying at least 10 min of pretreatment. This pretreatment allows to change chemical-physical and microbiological properties of wine lees, that are compatible as organic substrate for microalgae cultivation in a circular economy approach.

{"title":"Evaluation of wine lees ultrasonic cavitation as pre-treatment and its possible use as substrate for microalgae biomass production","authors":"Scarponi Paolina , Bravi Marco , Manente Sabrina , Cavinato Cristina","doi":"10.1016/j.algal.2025.103929","DOIUrl":"10.1016/j.algal.2025.103929","url":null,"abstract":"<div><div>Ultrasonic cavitation is a technology used to improve organic carbon solubilization of a substrate, increasing its biodegradability for further biological treatment. Moreover, its application has a pasteurization effect on waste stream. In this study, different cavitation times (5, 10, 20 and 30 min) are tested on white and red wine lees to evaluate the organic compounds solubilization, physical-chemical variation and antimicrobial cavitation effects, with the aim of further valorization with biotechnological applications such as microalgae growth. Wine lees cavitated effluents are applied as substrate for <em>Chlorella vulgaris</em> growth, obtaining a microalgae-yeast microbial community biomass of 2.4 ± 0.7 g L<sup>−1</sup> (WL) and 1.7 ± 0.0 g L<sup>−1</sup> (RL) with 33.9 ± 4.1 % (WL) and 60.5 ± 5.3 % (RL) of lipids content. Ultrasound cavitation is efficient in controlling native microorganism proliferation (yeast and mold) only applying at least 10 min of pretreatment. This pretreatment allows to change chemical-physical and microbiological properties of wine lees, that are compatible as organic substrate for microalgae cultivation in a circular economy approach.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"86 ","pages":"Article 103929"},"PeriodicalIF":4.6,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143143901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cyclic peptides from cyanobacteria: Structural insights, biological functions, and mechanisms of cyclization

IF 4.6 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2025-01-21 DOI: 10.1016/j.algal.2025.103925

Nuo Chen , Rui Huang , Hangzhen Lan , Jouni Jokela , Huamao Wei , Shuai Zhi , Liwei Liu

Cyclic peptides are highly interested in natural product research due to their unique chemical structures and diverse biological activities, which substantially contribute to drug development. Cyanobacteria are recognized as a prolific source of bioactive compounds and have contributed significantly to this field. Over the past decade, 142 novel cyanobacterial cyclic peptides and peptolides from cyanobacteria have been identified, revealing many novel chemicals. Recent advancements have provided new perspectives on the cyanobacteria cyclic peptides and peptolides. This review systematically elaborates on the latest research progress, summarizing the identified cyclic peptide and peptolide structures, biological activities, and cyclization mechanisms over the past decade (2014–2023). By providing critical insights, this review aims to facilitate the discovery of novel cyclic peptides and peptolides from cyanobacteria and guide future efforts in structural engineering.

引用次数: 0