Algal Research-Biomass Biofuels and Bioproducts最新文献

{"title":"Microalgae-derived biodiesel: Review on microalgae species, genetic modification, cultivation strategies, and mutagenesis","authors":"Jing Tian ,&nbsp;Jianqun Lin ,&nbsp;Guoqiang Zhuang ,&nbsp;Peiyao Sun ,&nbsp;Pengxue Geng ,&nbsp;Linxu Chen ,&nbsp;Xianke Chen","doi":"10.1016/j.algal.2025.104490","DOIUrl":"10.1016/j.algal.2025.104490","url":null,"abstract":"<div><div>The growing global demand for unconventional energy sources, particularly biofuels, is driven by fossil fuel depletion, climate change, energy security concerns, and policy incentives. Microalgae, as a third-generation biofuel feedstock, are an ideal candidate for biodiesel production due to their high CO₂ fixation capacity, high productivity, environmental sustainability, and favorable biodiesel properties. This review systematically evaluates diverse microalgal species, their lipid profiles, and fatty acid compositions to identify optimal strains for biodiesel synthesis. From a cultivation perspective, we examined the effects of key environmental parameters (such as temperature, irradiance, and CO₂ concentrations) on microalgal growth and lipid biosynthesis, with particular emphasis on the mechanisms regulating lipid productivity and fatty acid composition. Furthermore, building upon current knowledge of algal lipid metabolism, we discuss advanced genetic engineering strategies for targeted metabolic pathway modification to develop high-lipid mutants or transgenic strains. While genetic manipulation enables precise metabolic control, its widespread application is limited by technical complexity, high costs, and insufficient genomic information, especially for extremophilic species. Therefore, this review analyzes current achievements and challenges in microalgal genetic engineering while highlighting the advantages and future potential of random mutagenesis for developing novel algal strains for biodiesel production.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"93 ","pages":"Article 104490"},"PeriodicalIF":4.5,"publicationDate":"2025-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145836699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential of pyrolysis products of Enzyme-Treated vs. Intact Nannochloropsis gaditana: physicochemical characterization and antifungal activity","authors":"Wessal Ouedrhiri ,&nbsp;Abderrahman Mellalou ,&nbsp;Zouhair Ait Taleb ,&nbsp;Abderrahim Aasfar ,&nbsp;Bader Flissate ,&nbsp;Mariam Tounsi ,&nbsp;Abdelkader Outzourhit ,&nbsp;Hicham El-Arroussi ,&nbsp;Fouad Ghamouss","doi":"10.1016/j.algal.2025.104482","DOIUrl":"10.1016/j.algal.2025.104482","url":null,"abstract":"<div><div>This study examines the pyrolysis products derived from two types of <em>Nannochloropsis gaditana</em> biomass: untreated intact biomass (IntBA) and enzymatically pretreated biomass (EnzBA), with the latter regarded as a byproduct of the enzymatic extraction process. The analysis aims to evaluate how enzymatic pretreatment influences the yield and composition of the pyrolysis-derived products, providing insights into the valorization potential of enzymatic extraction residues. Comprehensive characterization of biochar and bio-oil derived from pyrolysis at 600 °C was conducted, highlighting the influence of the feedstock composition on product yields and quality. Bio-oil and biochar yields for IntBA were 51.7 % and 26.9 %, respectively, whereas EnzBA produced a higher biochar yield (55.3 %) but lower bio-oil yield (24.6 %). Physicochemical analyses, including FTIR, GC–MS, and TGA, revealed significant differences in the structural and chemical properties of the products. Notably, the biochar produced from IntBA exhibited superior thermal stability and a higher carbon content (65 %) compared to the biochar derived from EnzBA (49 %). The IntBA biochar also showed a BET surface area of 249 m²/g and a total pore volume of 0.39 cm³/g, whereas the EnzBA biochar displayed no pore development, with no measurable BET surface area and no observable porosity in the SEM images. Moreover, the IntBA biochar presented a high degree of structural disorder, evidenced by an ID/IG ratio of 0.94. In contrast, the bio-oils contained a variety of oxygenated and nitrogenous compounds suitable for biofuel applications. The IntBA and EnzBA bio-oils exhibited flash points of 130 °C and 135 °C and water contents of 21.3 % and 33.7 %, respectively. Additionally, antifungal assays revealed promising bioactivity, with pyrolyzed oils inhibiting fungal growth against <em>Botrytis cinerea</em>, <em>Fusarium oxysporum</em>, and <em>Fusarium solani</em>, showcasing potential in agricultural applications. This research underscores the dual benefits of enzymatic pretreatment and pyrolysis in valorizing microalgae biomass byproducts, offering sustainable avenues for bioenergy and bioproducts development.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"93 ","pages":"Article 104482"},"PeriodicalIF":4.5,"publicationDate":"2025-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145786529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and functional prediction of the C3H transcription factor family in Phaeodactylum tricornutum: Regulation of laminarin synthesis via circadian rhythm antagonistic interaction between PtC3H-1 and PtC3H-5","authors":"Kanglie Guo ,&nbsp;Zhitao Ye ,&nbsp;Xiaomin Wu ,&nbsp;Ruixin Li ,&nbsp;Zhen Wang ,&nbsp;Shuqi Wang ,&nbsp;Douglas R. Tocher ,&nbsp;Xiaojuan Liu","doi":"10.1016/j.algal.2025.104485","DOIUrl":"10.1016/j.algal.2025.104485","url":null,"abstract":"<div><div>The CCCH-structured transcription factors (C3H) play critical roles in growth, development, and stress responses in higher plants, yet research on the <em>C3H</em> gene family in microalgae remains unreported. In this study, 10 <em>PtC3H</em> members were identified from the marine diatom <em>P. tricornutum</em> through HMM search. Bioinformatic analyses predicted that members of this family play a pivotal role in RNA binding-mediated post-transcriptional regulation. Three distinct expression patterns were identified for <em>PtC3Hs</em> under a 12-hour light/dark cycle. Notably, the expression profile of <em>PtC3H-1</em> and <em>PtC3H-5</em> suggests that they may regulate the biosynthesis of laminarin through circadian rhythm. Further analysis employing three-dimensional structural modeling and molecular docking predicted that <em>PtC3H-1</em> may function through a dual mechanism: beyond RNA binding, it potentially activates the expression of downstream genes by interacting with <em>cis</em>-acting elements, thereby promoting laminarin synthesis. Concomitantly, the study found that <em>PtC3H-5</em> regulate laminarin metabolism through its interaction with <em>PtC3H-1</em>. Based on the above, this study not only accomplished systematic identification and functional prediction of all PtC3Hs in <em>P. tricornutum</em>, enriching the theoretical basis of this family in diatom, but also predicted that <em>PtC3H-1</em> and <em>PtC3H-5</em> synergistically mediate circadian rhythm to dynamically regulate laminarin metabolic homeostasis, along with their core molecular mechanisms.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"93 ","pages":"Article 104485"},"PeriodicalIF":4.5,"publicationDate":"2025-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145836602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photoregulation of life cycle, photosynthesis, growth and lutein production in Haematococcus lacustris (Volvocales, Chlorophyta)","authors":"Gabriela Vilvert Vansuita ,&nbsp;Bruna Rodrigues Moreira ,&nbsp;Willian da Silva Oliveira ,&nbsp;Cândice Maria Boff ,&nbsp;Julia Vega ,&nbsp;Nathalie Korbee ,&nbsp;Félix L. Figueroa ,&nbsp;Leonardo Rubi Rörig ,&nbsp;José Bonomi-Barufi","doi":"10.1016/j.algal.2025.104484","DOIUrl":"10.1016/j.algal.2025.104484","url":null,"abstract":"<div><div>Radiation is one of the most relevant parameters driving autotrophic organisms, such as <em>Haematococcus lacustris</em>, which possesses various life cycle stages in its life cycle and accumulates different carotenoids (mainly lutein and astaxanthin). In this study, twelve monochromatic LEDs and five irradiances were applied to evaluate morphophysiology and biochemical compounds focusing on the accumulation of lutein by early stages of <em>H. lacustris</em> life cycle. Photosynthesis, growth and life cycle stages, pigment quantification and antioxidant capacity were evaluated. Results showed the influence of radiation wavelength and irradiances on changes/alterations in life cycle stages, and different life cycle stages were prominent in some specific radiation wavelengths. Reddish flagellate cells and intermediate cysts were formed when the species received higher amounts of irradiance. Action spectra were obtained through electron transport rate (ETR), showing that ETR effectiveness decreased over time when the species was exposed to red light. Regarding pigment composition, chlorophylls <em>a</em> and <em>b</em> and the carotenoid lutein were detected, their contents being influenced both by radiation wavelength and irradiance. Negative correlations between biomass and all three pigments, as well as between biomass and the antioxidant capacity, were found. Meanwhile, lutein presented a strong positive correlation with the antioxidant capacity. This study features innovation and fundamental understanding on radiation regulating responses, allowing a future industrial upscaling and application of specific irradiance and wavelengths to generate relevant responses in the development of bioproducts, mainly the carotenoid lutein, as a further alternative to conventionally exploited resources.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"93 ","pages":"Article 104484"},"PeriodicalIF":4.5,"publicationDate":"2025-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145836705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-omics dissect the molecular mechanisms driving high-lipid production in a laboratory-evolved Chlamydomonas mutant","authors":"David R. Nelson ,&nbsp;Amphun Chaiboonchoe ,&nbsp;Weiqi Fu ,&nbsp;Basel Khraiwesh ,&nbsp;Bushra Dohai ,&nbsp;Ashish Jaiswal ,&nbsp;Dina Al-Khairy ,&nbsp;Alexandra Mystikou ,&nbsp;Latifa Al Nahyan ,&nbsp;Amnah Salem Alzahmi ,&nbsp;Layanne Nayfeh ,&nbsp;Sarah Daakour ,&nbsp;Matthew J. O'Connor ,&nbsp;Mehar Sultana ,&nbsp;Khaled M. Hazzouri ,&nbsp;Jean-Claude Twizere ,&nbsp;Kourosh Salehi-Ashtiani","doi":"10.1016/j.algal.2025.104479","DOIUrl":"10.1016/j.algal.2025.104479","url":null,"abstract":"<div><div>Enhancing lipid accumulation in microalgae is critical for commercial viability but often compromises growth. We previously generated through UV mutagenesis and iterative selection a <em>Chlamydomonas reinhardtii</em> mutant (H5) that retains parental growth while producing 3.2-fold more lipids (Sharma et al., 2015; Abdrabu et al., n.d.). Here, we present multi-omic analyses elucidating the molecular basis of this phenotype. Whole-genome sequencing revealed over 3000 mutations including a frameshift in the regulatory domain of 6-phosphofructokinase (PFK1). Six independent CLiP mutants in affected genes also showed elevated lipids, including a PFK1 mutant, validating functional relevance. Transcriptomics revealed upregulation of glycolytic genes and nutrient acquisition pathways under nutrient-replete conditions. Metabolomics identified an 8.31-fold malonate increase (<em>p</em> = 8.5 × 10⁻⁴), linking glycolysis to lipid synthesis. Lipidomics showed increased TAG diversity and lack of betaine lipids. Epigenomics revealed genome-wide hypermethylation, potentially stabilizing the phenotype. Together, these data suggest PFK1 deregulation drives metabolic reprogramming enabling lipid accumulation without growth penalty, demonstrating how evolutionary selection generates sophisticated metabolic solutions for engineering industrial microalgal strains.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"93 ","pages":"Article 104479"},"PeriodicalIF":4.5,"publicationDate":"2025-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145836601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HPLC quantification of guanine, hypoxanthine, xanthine, and uric acid in microalgae: The effects of different nitrogen sources on the purine profile of Cryptomonas maculata","authors":"Maximilian Bott,&nbsp;Anne Jantschke","doi":"10.1016/j.algal.2025.104483","DOIUrl":"10.1016/j.algal.2025.104483","url":null,"abstract":"<div><div>Microalgae are a substantial group of primary contributors to all aquatic reservoirs on earth. A recently observed strategy in their nitrogen metabolism is the ability to form purine-based crystalline inclusions that are often solid solutions. In microalgae, they are proposed to function as concentrated reservoirs of stable, nitrogen-containing, rapid-turnover metabolites or stress responses. Yet, many aspects in nitrogen-metabolization and compositional dynamics remain unclear.</div><div>To address this issue, an HPLC method for the quantification of all relevant purines (guanine, hypoxanthine, xanthine and uric acid) was designed and applied to 14 taxonomically different microalgae. <em>Cryptomonas maculata</em> was selected as a model species to study the effects of different nitrogen sources (NaNO₃ and urea) at lowered and increased concentrations on purine formation over time. This study is the first to address purine formation in a time-resolved manner and to analyze compositional dynamics.</div><div>One of the main results of the study is the high variability in uric acid concentrations, while the concentrations of guanine, xanthine, and hypoxanthine remain constant. The type of nitrogen source is shown to be more impacting on these concentrations than the actual concentration of nitrogen. Further, a significant amount of uric acid was produced during the initial phase of cultivation, followed by its subsequent degradation after 2 to 4 weeks.</div><div>These findings offer a first insight into the complex dynamics and responses of microalgae to environmental changes and hold considerable potential to elucidate a wide range of phenomena, including algae blooms and the dynamics of coral ecosystems.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"93 ","pages":"Article 104483"},"PeriodicalIF":4.5,"publicationDate":"2025-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145786478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ontogenetic shifts in photoprotective and antioxidant strategies determine high light resilience in the kelp Saccharina japonica","authors":"Guiyang Song ,&nbsp;Zhourui Liang ,&nbsp;Yanmin Yuan ,&nbsp;Wenjun Wang","doi":"10.1016/j.algal.2025.104476","DOIUrl":"10.1016/j.algal.2025.104476","url":null,"abstract":"<div><div>High light stress poses a major constraint on the growth and physiological performance of <em>Saccharina japonica</em>, often leading to thallus damage and reduced productivity. To inform stage-specific cultivation strategies, it is essential to understand how tolerance mechanisms evolve across developmental stages. This study systematically examined the photosynthetic and biochemical responses to high light stress across four representative stages of <em>S. japonica</em>: sporeling (5–8 cm in length), young sporophyte (20–30 cm), robust sporophyte (200–250 cm), and mature sporophyte (350–450 cm). They were exposed to high light (300, 600, and 1000 μmol photons m⁻² s⁻¹) for 1, 3, and 5 h, followed by a 24-h recovery period. Results revealed a pronounced ontogenetic gradient in stress tolerance. Sporeling and young sporophyte stages were highly susceptible to photoinhibition, as indicated by marked declines in the maximal and actual quantum yields of PSII and photosynthetic efficiency. This vulnerability was associated with weak antioxidant responses, characterized by limited upregulation of protective enzymes (e.g., ascorbate peroxidase) and significant reductions in total antioxidant capacity, thereby impeding PSII recovery. In contrast, the robust and mature sporophyte stages exhibited enhanced resilience, supported by two distinct, well-coordinated defense systems. The mature sporophyte stage maintained high photochemical performance through a combination of sustained regulated energy dissipation [Y(NPQ)] and strong antioxidant capacity involving both enzymatic (e.g., ascorbate peroxidase, glutathione peroxidase) and non-enzymatic (e.g., ascorbic acid, glutathione) components. The robust sporophyte stage, however, adopted a different strategy, compensating for minimal Y(NPQ) with a particularly powerful enzymatic antioxidant response. These findings demonstrate that high light tolerance in <em>S. japonica</em> is strongly dependent on ontogeny, underpinned by the integration and divergence of photoprotective and redox-balancing mechanisms. This ontogenetic plasticity provides a physiological foundation for refining light management practices and improving stage-targeted cultivation efficiency in <em>S. japonica</em> aquaculture.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"93 ","pages":"Article 104476"},"PeriodicalIF":4.5,"publicationDate":"2025-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145786530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Degradation dynamics and residues of sodium dodecyl benzene sulfonate as a novel pesticide for microalgal cultivation and its safety assessment","authors":"Aoqi Zhang ,&nbsp;Xiaobin Wen ,&nbsp;Zhongjie Wang ,&nbsp;Kaixuan Wang ,&nbsp;Yahong Geng ,&nbsp;Yeguang Li ,&nbsp;Yi Ding","doi":"10.1016/j.algal.2025.104477","DOIUrl":"10.1016/j.algal.2025.104477","url":null,"abstract":"<div><div>Mass cultivation of microalgae is crucial for microalgal resource utilization. However, it is frequently threatened by biological contamination, which causes substantial economic losses. We previously demonstrated the effectiveness of sodium dodecyl benzene sulfonate (SDBS) as a novel pesticide for biological contamination control. In this study, we investigated the degradation dynamics of SDBS in culture media, its potential residues in microalgal biomass, and its application safety under laboratory and outdoor raceway pond conditions. Additionally, effects of key physicochemical factors, including the microalgal species, temperature, light intensity, and aeration, on SDBS degradation were examined under sterile conditions. Aeration significantly enhanced SDBS degradation, achieving a 51.8 % reduction after 96 h, whereas variations in microalgal species, light intensity, and temperature had limited impact on its degradation. In non-sterile laboratory cultures, the degradation rate constants <em>k</em> of SDBS in <em>Chlorella pyrenoidosa</em>, <em>Graesiella emersonii</em>, and <em>Haematococcus pluvialis</em> cultures were 0.818 d⁻¹, 0.348 d⁻¹, and 0.512 d⁻¹, respectively, with terminal concentrations declining to 0.36, 0.30, and 0.37 mg/L. Field tests in 5-m² raceway ponds showed comparable dynamics, with reductions to 0.41 mg/L in <em>C. pyrenoidosa</em>, 0.46 mg/L in <em>G. emersonii</em>, and 0.25 mg/L in <em>H. pluvialis</em>, corresponding to degradation rates of 95.9, 95.4, and 96.4 %, respectively. Importantly, no SDBS residues were detected in the microalgal biomass under both the laboratory and field conditions. These results highlight the safety of SDBS for industrial-scale microalgal production and provide an effective strategy to address biological contamination. Nevertheless, further comprehensive risk assessments are required to determine its long-term environmental safety.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"93 ","pages":"Article 104477"},"PeriodicalIF":4.5,"publicationDate":"2025-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145786477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Amitriptyline toxicity mechanisms in Chlorella vulgaris and potential for bioremediation of contaminated wastewater","authors":"Wenjie Yuan,&nbsp;Yicheng Wu,&nbsp;Yeling Zhou,&nbsp;Bingjun Shu,&nbsp;Jinqi Cai,&nbsp;Haiyan Fu","doi":"10.1016/j.algal.2025.104475","DOIUrl":"10.1016/j.algal.2025.104475","url":null,"abstract":"<div><div>Traditional wastewater treatment processes have a relatively low removal rate of the tricyclic antidepressant amitriptyline. This study employed <em>Chlorella vulgaris</em> as the test organism to investigate the effects of exposure to different concentrations of amitriptyline on various biological functions of the microalga. The results showed that the physiological activities of <em>C. vulgaris</em> were inhibited by amitriptyline primarily through the disruption of photosynthesis, the imbalance of the antioxidant system, and the suppression of photosynthesis-related gene expression. The content of chlorophyll <em>a</em> was significantly reduced by 72.0 %, while the activities of superoxide dismutase and catalase were increased to 217.5 % and 67.2 %, respectively. The transcriptional levels of <em>PsaB</em> and <em>PsbC</em> genes were significantly suppressed by 75.5 % and 57.2 %, respectively. After 8 days of exposure to 5 mg/L amitriptyline, the maximum removal rate of amitriptyline by <em>C. vulgaris</em> was determined to be 62.62 %, with biodegradation identified as the predominant removal pathway. The removal of amitriptyline followed pseudo-first-order kinetics, with a degradation half-life of 5.93 days. The study provides theoretical foundations for optimizing microalgal based treatment processes of amitriptyline contaminated wastewater and delivers critical data support for environmental risk assessment of amitriptyline.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"93 ","pages":"Article 104475"},"PeriodicalIF":4.5,"publicationDate":"2025-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145786475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Subtleties of the synergistic inhibition of Microcystis aeruginosa by L-lysine and a flow-through copper ionization cell: Insights from physiology and transcriptomics","authors":"Yuewen Zhang ,&nbsp;Yujia Gao ,&nbsp;Yu Hong ,&nbsp;Man Liang ,&nbsp;Xing Xie","doi":"10.1016/j.algal.2025.104467","DOIUrl":"10.1016/j.algal.2025.104467","url":null,"abstract":"<div><div>The mitigation of <em>Microcystis aeruginosa</em> blooms is a critical environmental imperative that requires innovative technologies to effectively inhibit blooms. This study demonstrates that a synergistic approach combining L-lysine and a flow-through copper ionization cell achieved 84.39 % and 79.55 % maximum inhibition efficiency for toxic and non-toxic <em>M. aeruginosa</em>, respectively. Furthermore, the combined treatment demonstrated significant inhibitory effects on <em>M. aeruginosa</em> in natural water bodies, with total microcystin-LR (MC-LR) and intracellular MC-LR concentrations decreasing by 90 % and 98.84 %, respectively. A total of 2028 and 1805 differentially expressed genes (DEGs) were identified in toxic and non-toxic <em>M. aeruginosa</em>. The integrity of the cell membrane was notably compromised, leading to the downregulation of genes associated with photosynthesis, which in turn limited the synthesis of phycobiliprotein, chlorophyll, and ATP, as well as the proton transfer process. Moreover, energy production was inhibited, resulting in the downregulation of genes related to oxidative phosphorylation. Overall, this research provides a comprehensive understanding of the mechanisms underlying the control of <em>M. aeruginosa</em> under combined treatment with L-lysine and Cu ions, offering a novel approach to addressing cyanobacterial blooms in the future.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"93 ","pages":"Article 104467"},"PeriodicalIF":4.5,"publicationDate":"2025-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145836701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}