Algal Research-Biomass Biofuels and Bioproducts最新文献_第9页

Co-utilization of bread waste and harmful algal bloom-derived cyanobacterial biomass for Euglena gracilis cultivation: Turning waste and pollution into resources 面包废弃物与有害藻华蓝藻生物质在绿豆栽培中的共同利用：将废物和污染转化为资源

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2025-12-31 DOI: 10.1016/j.algal.2025.104510

Woo Shik Jung , Yugeun Jung , Jee Young Kim , Eun Hee Bae , Yoon-E Choi

Microcystis aeruginosa is a harmful algal bloom (HAB)-forming cyanobacterium that poses significant environmental risks. Additionally, a large amount of bread waste rich in valuable carbon is discarded worldwide, resulting in both environmental and economic challenges. In this study, we sought to utilise M. aeruginosa biomass and bread waste for the cultivation of the beneficial microalga Euglena gracilis, thereby converting both HAB biomass and food waste into valuable resources. To this end, algal hydrolysate (AH) derived from M. aeruginosa and bread waste hydrolysate (BWH) were incorporated as components of an algal culture medium. Various proportions of AH were mixed with BWH, and the productivity of E. gracilis was assessed. Among all tested conditions, the 20 % AH medium resulted in the highest dry cell weight (9.01 g) of E. gracilis. During the subsequent bioreactor scale-up, biomass accumulation further increased to 9.87 g. Metabolite production was then compared under aerobic and anaerobic conditions using both AH and BWH based media. When cultivation transitioned from aerobic to anaerobic conditions, the lipid content of E. gracilis biomass increased from 12.2 % to 26.6 %. Furthermore, the fatty acid profile shifted toward a composition more suitable for jet fuel applications. This study presents a novel strategy demonstrating that both environmental pollutants (e.g., AH derived from HABs) and food by-products or waste (e.g., BWH) can serve as effective nutrient sources for microalgal cultivation, offering a promising approach for more economical and environmentally sustainable microalgae-based biotechnologies.

铜绿微囊藻是一种有害的藻华（HAB）形成蓝藻，造成重大的环境风险。此外，大量富含有价值碳的面包废料在世界范围内被丢弃，造成了环境和经济挑战。在这项研究中，我们寻求利用铜绿假单胞菌生物量和面包垃圾培养有益的微藻绿藻，从而将有害藻华生物量和食物垃圾转化为有价值的资源。为此，从铜绿假单胞菌中提取的藻类水解物（AH）和面包废物水解物（BWH）作为藻类培养基的组成部分。将不同比例的水杨酸与牛膝水杨酸混合，评价牛膝水杨酸的产率。在所有测试条件下，20%的AH培养基使薄叶菊的干细胞质量最高（9.01 g）。在随后的生物反应器放大过程中，生物量积累进一步增加到9.87 g。然后比较了在有氧和厌氧条件下使用AH和BWH为基础的培养基的代谢物产量。当培养从好氧条件过渡到厌氧条件时，凤尾草生物量的脂质含量从12.2%增加到26.6%。此外，脂肪酸谱转向了更适合航空燃料应用的组合物。本研究提出了一种新的策略，表明环境污染物（例如，从有害藻华中提取的AH）和食物副产品或废物（例如，BWH）都可以作为微藻培养的有效营养来源，为更经济和环境可持续的微藻生物技术提供了一种有前途的方法。

{"title":"Co-utilization of bread waste and harmful algal bloom-derived cyanobacterial biomass for Euglena gracilis cultivation: Turning waste and pollution into resources","authors":"Woo Shik Jung , Yugeun Jung , Jee Young Kim , Eun Hee Bae , Yoon-E Choi","doi":"10.1016/j.algal.2025.104510","DOIUrl":"10.1016/j.algal.2025.104510","url":null,"abstract":"<div><div><em>Microcystis aeruginosa</em> is a harmful algal bloom (HAB)-forming cyanobacterium that poses significant environmental risks. Additionally, a large amount of bread waste rich in valuable carbon is discarded worldwide, resulting in both environmental and economic challenges. In this study, we sought to utilise <em>M. aeruginosa</em> biomass and bread waste for the cultivation of the beneficial microalga <em>Euglena gracilis</em>, thereby converting both HAB biomass and food waste into valuable resources. To this end, algal hydrolysate (AH) derived from <em>M. aeruginosa</em> and bread waste hydrolysate (BWH) were incorporated as components of an algal culture medium. Various proportions of AH were mixed with BWH, and the productivity of <em>E. gracilis</em> was assessed. Among all tested conditions, the 20 % AH medium resulted in the highest dry cell weight (9.01 g) of <em>E. gracilis</em>. During the subsequent bioreactor scale-up, biomass accumulation further increased to 9.87 g. Metabolite production was then compared under aerobic and anaerobic conditions using both AH and BWH based media. When cultivation transitioned from aerobic to anaerobic conditions, the lipid content of <em>E. gracilis</em> biomass increased from 12.2 % to 26.6 %. Furthermore, the fatty acid profile shifted toward a composition more suitable for jet fuel applications. This study presents a novel strategy demonstrating that both environmental pollutants (e.g., AH derived from HABs) and food by-products or waste (e.g., BWH) can serve as effective nutrient sources for microalgal cultivation, offering a promising approach for more economical and environmentally sustainable microalgae-based biotechnologies.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"94 ","pages":"Article 104510"},"PeriodicalIF":4.5,"publicationDate":"2025-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145923350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the associations among halogenated compounds in Asparagopsis taxiformis: temporal shifts and bacterial impacts under intensive cultivation 探索taxxiformasopsis中卤化化合物之间的关系：在集约化栽培下的时间变化和细菌影响

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2025-12-30 DOI: 10.1016/j.algal.2025.104513

S. Blanco-González , P.R. Brooks , D.A. Heyne , A.H. Campbell , S.F. Cummins , N.A. Paul

Bromoform is the key antimethanogenic bioactive in Asparagopsis species, yet its variation in culture and relationship with other halogenated compounds remain poorly understood. Here, the profile of 9 halogenated compounds in A. taxiformis sporophytes cultured at 0.5 g/L over five weeks were quantified, comparing control cultures to those exposed to gentamicin (10 and 20 mg/L) which temporarily reduced surface bacteria by 81–95 %. Chemical analyses (n = 75) identified three classes of compounds (haloacids, halomethanes and haloacetones) comprising totals of 1–3.3 % dry weight (dw). Haloacids represented half the total; dibromoacetic acid (DBAA, 31.4 %), tribromoacetic acid (TBAA, 10 %) and bromochloroacetic acid (1.3 %). Halomethanes accounted for 39.5 % with bromoform (36.4 %) the single most abundant compound at 0.44–1.38 % dw. DBAA and TBAA concentrations reached 0.47–0.82 % and 0.13–0.38 % dw, respectively. Haloacetones contributed 10.7 % of the total, positively correlating with five other halogenated compounds. However, DBAA and TBAA concentrations correlated more closely to growth, cell morphology and bacterial densities than to other halogenated compounds. Overall, there was a temporal shift in growth rates for both antibiotic treatments – initially suppressing growth by 10–25 % before stabilising with all treatments converging to a specific growth rate of ~8.5 % day⁻¹ by week 5. Extending the cultures for an additional 10 weeks confirmed no long-term impacts of antibiotic treatments on growth, halogenated compounds or the composition of surface bacterial community. This study is the first comprehensive evaluation of halogenated compound loads in cultured Asparagopsis, identifying TBAA as a major component that may contribute to antimethanogenic activity in ruminant feed.

溴仿是天冬酰胺中主要的抗甲烷生物活性物质，但其在培养过程中的变化及其与其他卤化化合物的关系尚不清楚。本研究对0.5 g/L浓度下培养5周的A. taxformis孢子体中9种卤化化合物的分布进行了量化，并将对照培养物与暴露于庆大霉素（10和20 mg/L）的培养物进行了比较，庆大霉素暂时减少了81 - 95%的表面细菌。化学分析（n = 75）确定了三类化合物（卤酸、卤甲烷和卤丙酮），总含量为干重（dw）的1 - 3.3%。卤代酸占总数的一半；二溴乙酸（DBAA, 31.4%）、三溴乙酸（TBAA, 10%）和溴氯乙酸（1.3%）。卤甲烷占39.5%，溴仿占36.4%，含量最高，为0.44 - 1.38%。DBAA和TBAA浓度分别为0.47 ~ 0.82%和0.13 ~ 0.38%。卤代酮贡献了10.7%的总量，与其他五种卤代化合物正相关。然而，与其他卤化化合物相比，DBAA和TBAA浓度与生长、细胞形态和细菌密度的关系更为密切。总的来说，两种抗生素治疗的生长速度都有时间变化——最初抑制生长10 - 25%，然后稳定下来，所有治疗在第5周收敛到约8.5%的特定生长速度。将培养物再延长10周，证实抗生素治疗对生长、卤化化合物或表面细菌群落的组成没有长期影响。本研究首次对培养的天冬酰胺中卤化化合物负荷进行了综合评价，确定了TBAA可能是反刍动物饲料中抗甲烷活性的主要成分。

{"title":"Exploring the associations among halogenated compounds in Asparagopsis taxiformis: temporal shifts and bacterial impacts under intensive cultivation","authors":"S. Blanco-González , P.R. Brooks , D.A. Heyne , A.H. Campbell , S.F. Cummins , N.A. Paul","doi":"10.1016/j.algal.2025.104513","DOIUrl":"10.1016/j.algal.2025.104513","url":null,"abstract":"<div><div>Bromoform is the key antimethanogenic bioactive in <em>Asparagopsis</em> species, yet its variation in culture and relationship with other halogenated compounds remain poorly understood. Here, the profile of 9 halogenated compounds in <em>A. taxiformis</em> sporophytes cultured at 0.5 g/L over five weeks were quantified, comparing control cultures to those exposed to gentamicin (10 and 20 mg/L) which temporarily reduced surface bacteria by 81–95 %. Chemical analyses (<em>n</em> = 75) identified three classes of compounds (haloacids, halomethanes and haloacetones) comprising totals of 1–3.3 % dry weight (dw). Haloacids represented half the total; dibromoacetic acid (DBAA, 31.4 %), tribromoacetic acid (TBAA, 10 %) and bromochloroacetic acid (1.3 %). Halomethanes accounted for 39.5 % with bromoform (36.4 %) the single most abundant compound at 0.44–1.38 % dw. DBAA and TBAA concentrations reached 0.47–0.82 % and 0.13–0.38 % dw, respectively. Haloacetones contributed 10.7 % of the total, positively correlating with five other halogenated compounds. However, DBAA and TBAA concentrations correlated more closely to growth, cell morphology and bacterial densities than to other halogenated compounds. Overall, there was a temporal shift in growth rates for both antibiotic treatments – initially suppressing growth by 10–25 % before stabilising with all treatments converging to a specific growth rate of ~8.5 % day<sup>−1</sup> by week 5. Extending the cultures for an additional 10 weeks confirmed no long-term impacts of antibiotic treatments on growth, halogenated compounds or the composition of surface bacterial community. This study is the first comprehensive evaluation of halogenated compound loads in cultured <em>Asparagopsis</em>, identifying TBAA as a major component that may contribute to antimethanogenic activity in ruminant feed.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"94 ","pages":"Article 104513"},"PeriodicalIF":4.5,"publicationDate":"2025-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145923279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Symbiotic interaction of Chlorella sp. and Shinella sp. for enhanced dissolved organic matter removal from sterilized mixed wastewater 小球藻和Shinella的共生作用对混合废水中溶解性有机物的去除效果

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2025-12-30 DOI: 10.1016/j.algal.2025.104501

Rumeng Lu , Qiaoyun Huang , Yunpu Wang , Xian Cui , Zhigang Yu , Naif Abdullah Al-Dhabi , Xiang Wang , Roger Ruan , Wangwang Tang , Zihan Li , Ting Zhou , Yuhuan Liu , Qi Zhang

Microalgae-bacteria symbiont (ABS) presents promising applications in wastewater treatment. However, the synergistic mechanisms underlying pollutants removal by ABS, particularly for dissolved organic matter (DOM), remain inadequately understood. This study evaluated the performance of a symbiotic system of Chlorella sp. and Shinella sp. in different real wastewater scenarios (WW1: mixture of anaerobic digestion effluent (ADE); WW2: mixture of ADE and Baijiu wastewater) across different hydraulic retention times (HRTs). Results demonstrate that ABS significantly outperformed both monocultures, achieving not only markedly higher biomass production (up to 535 % increase) and pollutant removal, but also exhibiting uniquely enhanced capacity for removing DOM components. Notably, these enhanced-removal DOM constituents accounted for over 20 % of the total differential DOM abundance in both wastewater types. Spectroscopic and UPLC-MS/MS analyses revealed that this enhanced performance was driven by active metabolite exchange between microalgae and bacteria, with amino acids, B vitamins, phytohormones (e.g., indoleacetic acid and gibberellins), and signaling molecules such as jasmonic acid underpinning this synergistic relationship. These interactions collectively regulated microbial growth, metabolism, and oxidative stress, thereby improving DOM removal. The resulting biomass was rich in proteins (60 %) and C16–18 fatty acids (96.34 %), thus representing a promising candidate for valorization. This study elucidates the metabolic and signaling mechanisms underlying the enhanced DOM removal in ABS, offering insights for scalable wastewater bioremediation and resource recovery.

微藻-细菌共生体（ABS）在污水处理中具有广阔的应用前景。然而，ABS去除污染物的协同机制，特别是对溶解有机物（DOM）的去除，仍然没有得到充分的了解。本研究评估了小球藻（Chlorella sp.）和Shinella sp.共生系统在不同实际废水场景中的性能(WW1：厌氧消化出水（ADE）的混合物；二战：ADE和白酒废水的混合物)在不同的水力停留时间（HRTs）。结果表明，ABS显著优于两种单一培养，不仅实现了显著更高的生物质产量（高达535%）和污染物去除，而且还表现出独特的增强的去除DOM成分的能力。值得注意的是，这些增强去除的DOM成分占两种废水中总DOM丰度差异的20%以上。光谱和UPLC-MS/MS分析表明，微藻和细菌之间的代谢物交换是微藻性能增强的主要原因，氨基酸、B族维生素、植物激素（如吲哚乙酸和赤霉素）和茉莉酸等信号分子支撑了这种协同关系。这些相互作用共同调节微生物生长、代谢和氧化应激，从而改善DOM的去除。所得到的生物量富含蛋白质（60%）和C16-18脂肪酸（96.34%），因此代表了有希望的增值候选者。本研究阐明了ABS中增强DOM去除的代谢和信号机制，为大规模废水生物修复和资源回收提供了见解。

{"title":"Symbiotic interaction of Chlorella sp. and Shinella sp. for enhanced dissolved organic matter removal from sterilized mixed wastewater","authors":"Rumeng Lu , Qiaoyun Huang , Yunpu Wang , Xian Cui , Zhigang Yu , Naif Abdullah Al-Dhabi , Xiang Wang , Roger Ruan , Wangwang Tang , Zihan Li , Ting Zhou , Yuhuan Liu , Qi Zhang","doi":"10.1016/j.algal.2025.104501","DOIUrl":"10.1016/j.algal.2025.104501","url":null,"abstract":"<div><div>Microalgae-bacteria symbiont (ABS) presents promising applications in wastewater treatment. However, the synergistic mechanisms underlying pollutants removal by ABS, particularly for dissolved organic matter (DOM), remain inadequately understood. This study evaluated the performance of a symbiotic system of <em>Chlorella</em> sp. and <em>Shinella</em> sp. in different real wastewater scenarios (WW1: mixture of anaerobic digestion effluent (ADE); WW2: mixture of ADE and Baijiu wastewater) across different hydraulic retention times (HRTs). Results demonstrate that ABS significantly outperformed both monocultures, achieving not only markedly higher biomass production (up to 535 % increase) and pollutant removal, but also exhibiting uniquely enhanced capacity for removing DOM components. Notably, these enhanced-removal DOM constituents accounted for over 20 % of the total differential DOM abundance in both wastewater types. Spectroscopic and UPLC-MS/MS analyses revealed that this enhanced performance was driven by active metabolite exchange between microalgae and bacteria, with amino acids, B vitamins, phytohormones (e.g., indoleacetic acid and gibberellins), and signaling molecules such as jasmonic acid underpinning this synergistic relationship. These interactions collectively regulated microbial growth, metabolism, and oxidative stress, thereby improving DOM removal. The resulting biomass was rich in proteins (60 %) and C16–18 fatty acids (96.34 %), thus representing a promising candidate for valorization. This study elucidates the metabolic and signaling mechanisms underlying the enhanced DOM removal in ABS, offering insights for scalable wastewater bioremediation and resource recovery.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"94 ","pages":"Article 104501"},"PeriodicalIF":4.5,"publicationDate":"2025-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146074051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A hybrid approach for photobioreactor modeling in CO2 biofixation using microalgae cultivation 利用微藻培养进行CO2生物固定的光生物反应器模拟的混合方法

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2025-12-29 DOI: 10.1016/j.algal.2025.104503

Shabnam Shahhoseyni , Mohammad Rahmani , Abhishek Sivaram

Carbon capture plays a key role in mitigating climate change, with microalgae offering a promising solution. This study presents a modeling framework for photobioreactors by integrating key phenomena involved in microalgae-based biomanufacturing. Specifically, a unified approach is proposed to simulate microalgae growth, incorporating growth kinetics, interphase mass transfer, light distribution, and hydrodynamics behavior. The framework combines physical, biological and data-driven models: gas hold-up is estimated using data-driven methods; light distribution is described by the P1radiation model; interphase mass transfer quantifies CO₂ absorption under light and dark phases; and microalgae growth kinetics are captured through a hybrid formulation with a correction mechanism to enhance prediction accuracy. The model structure and implementation are demonstrated using experimental data from previously published study, achieving MAPE values below 11 % across all experimental runs. This approach has the potential to support scale-up efforts and contribute to more efficient and sustainable biomanufacturing processes.

碳捕获在减缓气候变化方面发挥着关键作用，微藻提供了一个有希望的解决方案。本研究通过整合微藻生物制造中涉及的关键现象，提出了光生物反应器的建模框架。具体来说，我们提出了一种统一的方法来模拟微藻的生长，包括生长动力学、相间传质、光分布和水动力学行为。该框架结合了物理、生物和数据驱动模型：利用数据驱动方法估算气含率；光分布由p1辐射模型描述；相间传质量化了光相和暗相下的CO2吸收；并通过带有校正机制的混合配方捕捉微藻生长动力学，提高预测精度。使用先前发表的研究的实验数据证明了模型的结构和实现，在所有实验运行中实现了低于11%的MAPE值。这种方法有可能支持扩大规模的努力，并有助于更有效和可持续的生物制造过程。

{"title":"A hybrid approach for photobioreactor modeling in CO2 biofixation using microalgae cultivation","authors":"Shabnam Shahhoseyni , Mohammad Rahmani , Abhishek Sivaram","doi":"10.1016/j.algal.2025.104503","DOIUrl":"10.1016/j.algal.2025.104503","url":null,"abstract":"<div><div>Carbon capture plays a key role in mitigating climate change, with microalgae offering a promising solution. This study presents a modeling framework for photobioreactors by integrating key phenomena involved in microalgae-based biomanufacturing. Specifically, a unified approach is proposed to simulate microalgae growth, incorporating growth kinetics, interphase mass transfer, light distribution, and hydrodynamics behavior. The framework combines physical, biological and data-driven models: gas hold-up is estimated using data-driven methods; light distribution is described by the P1radiation model; interphase mass transfer quantifies CO<sub>2</sub> absorption under light and dark phases; and microalgae growth kinetics are captured through a hybrid formulation with a correction mechanism to enhance prediction accuracy. The model structure and implementation are demonstrated using experimental data from previously published study, achieving MAPE values below 11 % across all experimental runs. This approach has the potential to support scale-up efforts and contribute to more efficient and sustainable biomanufacturing processes.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"94 ","pages":"Article 104503"},"PeriodicalIF":4.5,"publicationDate":"2025-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145923347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Persistence of the Ulva prolifera Green Tide: eDNA technology reveals spatiotemporal variations in micropropagules in the Southern Shandong Peninsula 藻绿潮的持续：eDNA技术揭示了山东半岛南部微繁殖体的时空变化

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2025-12-29 DOI: 10.1016/j.algal.2025.104502

Faqing He , Yinqing Zeng , Qingqing Mu , Runze Chen , Zehua Chen , Shulin Tan , Huanhong Ji , Lihua Xia , Lining Xia , Yutao Qin , Peimin He , Jianheng Zhang

The frequent occurrence of green tides in the Yellow Sea poses a significant threat to both coastal ecosystems and local economies. Traditional monitoring methods struggle to accurately assess the dynamics of Ulva prolifera micropropagules due to their delayed response, limiting early prevention and control efforts. For proactive observation of green tides to better understand their biological invasion, this study applied environmental DNA (eDNA) technology for the first time to investigate the attached of Ulva along the southern coast of the Shandong Peninsula. Surveys at 18 stations were carried out in November 2023 and March 2024. High-throughput sequencing and molecular identification were used to analyze the spatial and temporal distribution patterns of macroalgae micropropagules and the underlying ecological drivers. The results showed that, compared with autumn, the relative sequence abundance of Chlorophyta increased in spring, with a 180-fold increase in eDNA signal in Longwan, Langya Town, linked to the disappearance of attached macroalgae. This shift indicated a seasonal ecological niche shift of U. prolifera from “benthic anchorage” to “floating proliferation”, highlighting a potential outbreak risk area. Correlation network analysis showed that U. prolifera was positively correlated with other green macroalgae species and certain protists (e.g., Massisteria marina), suggesting its potential influence on community structure through changes in habitat conditions and food chain dynamics. This study confirms that eDNA technology can effectively capture the dynamics of micropropagules in real-time, providing valuable data for early warning of green tides.

黄海绿潮的频繁发生对沿海生态系统和当地经济都构成了重大威胁。传统的监测方法难以准确评估增生Ulva微繁殖体的动态，因为它们的反应延迟，限制了早期预防和控制的努力。本研究首次应用环境DNA （environmental DNA, eDNA）技术，对山东半岛南部沿海的绿潮藻的附着情况进行了研究。在2023年11月和2024年3月进行了18个站点的调查。利用高通量测序和分子鉴定技术，分析了大藻微繁殖体的时空分布格局及其生态驱动因素。结果表明，与秋季相比，绿藻的相对序列丰度在春季有所增加，琅崖镇龙湾的eDNA信号增加了180倍，这与附着的大型藻类消失有关。这一变化表明，该物种的生态位发生了季节性变化，从“底栖栖地”向“漂浮增殖”转变，突出了潜在的暴发风险区域。相关网络分析结果表明，U. prolifera与其他大型绿藻物种及部分原生生物（如Massisteria marina）呈显著正相关，表明U. prolifera可能通过改变生境条件和食物链动态对群落结构产生影响。本研究证实了eDNA技术可以有效实时捕捉微繁殖体的动态，为绿潮预警提供有价值的数据。

{"title":"Persistence of the Ulva prolifera Green Tide: eDNA technology reveals spatiotemporal variations in micropropagules in the Southern Shandong Peninsula","authors":"Faqing He , Yinqing Zeng , Qingqing Mu , Runze Chen , Zehua Chen , Shulin Tan , Huanhong Ji , Lihua Xia , Lining Xia , Yutao Qin , Peimin He , Jianheng Zhang","doi":"10.1016/j.algal.2025.104502","DOIUrl":"10.1016/j.algal.2025.104502","url":null,"abstract":"<div><div>The frequent occurrence of green tides in the Yellow Sea poses a significant threat to both coastal ecosystems and local economies. Traditional monitoring methods struggle to accurately assess the dynamics of <em>Ulva prolifera</em> micropropagules due to their delayed response, limiting early prevention and control efforts. For proactive observation of green tides to better understand their biological invasion, this study applied environmental DNA (eDNA) technology for the first time to investigate the attached of <em>Ulva</em> along the southern coast of the Shandong Peninsula. Surveys at 18 stations were carried out in November 2023 and March 2024. High-throughput sequencing and molecular identification were used to analyze the spatial and temporal distribution patterns of macroalgae micropropagules and the underlying ecological drivers. The results showed that, compared with autumn, the relative sequence abundance of Chlorophyta increased in spring, with a 180-fold increase in eDNA signal in Longwan, Langya Town, linked to the disappearance of attached macroalgae. This shift indicated a seasonal ecological niche shift of <em>U. prolifera</em> from “benthic anchorage” to “floating proliferation”, highlighting a potential outbreak risk area. Correlation network analysis showed that <em>U. prolifera</em> was positively correlated with other green macroalgae species and certain protists (e.g., <em>Massisteria marina</em>), suggesting its potential influence on community structure through changes in habitat conditions and food chain dynamics. This study confirms that eDNA technology can effectively capture the dynamics of micropropagules in real-time, providing valuable data for early warning of green tides.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"94 ","pages":"Article 104502"},"PeriodicalIF":4.5,"publicationDate":"2025-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145974303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An overview of algal microbial desalination cell: Electron transfer mechanism, onsite and downstream applications and challenges 海藻微生物脱盐电池综述：电子传递机制，现场和下游应用和挑战

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2025-12-29 DOI: 10.1016/j.algal.2025.104504

Dhandapani Murugan, Kalaichelvi Ponnusamy, Samsudeen Naina Mohamed

Microbial desalination cell (MDC) is an emerging technology that simultaneously produces electricity from wastewater and treats saline water. However, expensive chemical catalyst is used to enhance the MDC performance which leads to unsustainability and environmental impact. The biocathode is an alternative and novel component in an ecofriendly manner to improve the MDC performance. In biocathode MDC, the various species such as bacteria, algae, yeast, or fungi acts as biocatalysts for reduction reaction by the formation of biofilm on the cathode. Predominantly, algae are used as a biocathode in the MDC for providing oxygen through the photosynthesis process by utilizing CO₂. The main objective of this work is to overview the potential application of algae in the cathode side of MDC through both onsite and downstream approaches towards biorefinery framework. This review also gives key aspects of Algal MDC, including, electron transfer mechanisms, cathodic oxygen production and reduction mechanisms and economic feasibility. This review highlights that the significance of algae to improve system performance and sustainability in a cost-effective manner, while eliminating the need for external energy input. Furthermore, it offers a sustainable solution for the simultaneous enables bioelectricity production, desalination and nutrient removal in both sides, along with biomass generation for value-added products.

微生物海水淡化电池（MDC）是一种利用废水发电并同时处理咸水的新兴技术。然而，为了提高MDC的性能，使用了昂贵的化学催化剂，导致不可持续性和对环境的影响。生物阴极是一种可替代的新型组件，以一种环保的方式来提高MDC的性能。在生物阴极MDC中，细菌、藻类、酵母或真菌等多种生物通过在阴极上形成生物膜来充当还原反应的生物催化剂。在MDC中，藻类主要被用作生物阴极，通过利用CO₂的光合作用过程提供氧气。这项工作的主要目的是概述藻类在MDC阴极侧的潜在应用，通过现场和下游方法实现生物炼制框架。本文综述了藻类多氧转化的主要研究方向，包括电子转移机制、阴极氧生成和还原机制以及经济可行性。这篇综述强调了藻类在以经济有效的方式提高系统性能和可持续性方面的重要性，同时消除了对外部能源输入的需求。此外，它提供了一个可持续的解决方案，同时实现生物发电，海水淡化和营养物去除，以及用于增值产品的生物质发电。

{"title":"An overview of algal microbial desalination cell: Electron transfer mechanism, onsite and downstream applications and challenges","authors":"Dhandapani Murugan, Kalaichelvi Ponnusamy, Samsudeen Naina Mohamed","doi":"10.1016/j.algal.2025.104504","DOIUrl":"10.1016/j.algal.2025.104504","url":null,"abstract":"<div><div>Microbial desalination cell (MDC) is an emerging technology that simultaneously produces electricity from wastewater and treats saline water. However, expensive chemical catalyst is used to enhance the MDC performance which leads to unsustainability and environmental impact. The biocathode is an alternative and novel component in an ecofriendly manner to improve the MDC performance. In biocathode MDC, the various species such as bacteria, algae, yeast, or fungi acts as biocatalysts for reduction reaction by the formation of biofilm on the cathode. Predominantly, algae are used as a biocathode in the MDC for providing oxygen through the photosynthesis process by utilizing CO₂. The main objective of this work is to overview the potential application of algae in the cathode side of MDC through both onsite and downstream approaches towards biorefinery framework. This review also gives key aspects of Algal MDC, including, electron transfer mechanisms, cathodic oxygen production and reduction mechanisms and economic feasibility. This review highlights that the significance of algae to improve system performance and sustainability in a cost-effective manner, while eliminating the need for external energy input. Furthermore, it offers a sustainable solution for the simultaneous enables bioelectricity production, desalination and nutrient removal in both sides, along with biomass generation for value-added products.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"94 ","pages":"Article 104504"},"PeriodicalIF":4.5,"publicationDate":"2025-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145973854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Maternal inheritance of photoperiodic responsiveness controls sporophyte maturation in Saccharina japonica: Implications for hybrid kelp breeding and seedling production 母系光周期响应遗传控制日本糖藻孢子体成熟：对杂交海带育种和幼苗生产的启示

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2025-12-23 DOI: 10.1016/j.algal.2025.104493

Jing Li , Xiaodong Li , Li Su , Lirong Chang , Yan Zhang , Ying Sun , Luyang Xiao , Ershuai Huang , Shaojun Pang

Reproductive timing in Saccharina japonica is tightly regulated by photoperiod, yet the genetic mechanism underlying variation in photoperiodic responsiveness remains unclear. This study examined whether photoperiod-dependent sporogenesis is maternally inherited in hybrid sporophytes. Two female gametophyte lines with contrasting maturation phenotypes—early-maturing (day-neutral) and late-maturing (short-day dependent)—were crossed with 22 genetically distinct male gametophytes. The resulting hybrid sporophytes were cultured under both natural and short-day conditions to evaluate reproductive differentiation. Hybrids derived from the early-maturing maternal line developed sori under natural photoperiods, whereas those from the late-maturing line required short-day induction to initiate sorus formation. Histological observations confirmed complete sorus morphogenesis and the release of viable meiospores following short-day exposure. These findings provide direct evidence that photoperiodic responsiveness in S. japonica is genetically determined and transmitted through the maternal lineage. This discovery reveals a novel mechanism of reproductive control in kelps and provides a conceptual framework for integrating photoperiod genetics into the development of cultivars with synchronized reproductive timing for commercial aquaculture.

甘蔗的生殖时间受到光周期的严格调控，但光周期响应性变异的遗传机制尚不清楚。本研究考察了杂交孢子体中依赖光周期的孢子发生是否由母系遗传。将早熟（昼中性）和晚熟（昼短依赖性）两种成熟表型不同的雌性配子体系与22个遗传上不同的雄性配子体杂交。杂种孢子体在自然和短日照条件下培养，以评估生殖分化。来自早熟母系的杂种在自然光周期下形成sori，而来自晚熟系的杂种则需要短时间的诱导才能形成sori。组织学观察证实，在短时间暴露后，孢子形成完整，并释放出活的减数孢子。这些发现提供了直接证据，表明日本稻的光周期反应是由遗传决定的，并通过母系传播。这一发现揭示了一种新的海带繁殖控制机制，并为将光周期遗传学整合到商业养殖中具有同步繁殖时间的品种的开发提供了一个概念框架。

{"title":"Maternal inheritance of photoperiodic responsiveness controls sporophyte maturation in Saccharina japonica: Implications for hybrid kelp breeding and seedling production","authors":"Jing Li , Xiaodong Li , Li Su , Lirong Chang , Yan Zhang , Ying Sun , Luyang Xiao , Ershuai Huang , Shaojun Pang","doi":"10.1016/j.algal.2025.104493","DOIUrl":"10.1016/j.algal.2025.104493","url":null,"abstract":"<div><div>Reproductive timing in <em>Saccharina japonica</em> is tightly regulated by photoperiod, yet the genetic mechanism underlying variation in photoperiodic responsiveness remains unclear. This study examined whether photoperiod-dependent sporogenesis is maternally inherited in hybrid sporophytes. Two female gametophyte lines with contrasting maturation phenotypes—early-maturing (day-neutral) and late-maturing (short-day dependent)—were crossed with 22 genetically distinct male gametophytes. The resulting hybrid sporophytes were cultured under both natural and short-day conditions to evaluate reproductive differentiation. Hybrids derived from the early-maturing maternal line developed sori under natural photoperiods, whereas those from the late-maturing line required short-day induction to initiate sorus formation. Histological observations confirmed complete sorus morphogenesis and the release of viable meiospores following short-day exposure. These findings provide direct evidence that photoperiodic responsiveness in <em>S. japonica</em> is genetically determined and transmitted through the maternal lineage. This discovery reveals a novel mechanism of reproductive control in kelps and provides a conceptual framework for integrating photoperiod genetics into the development of cultivars with synchronized reproductive timing for commercial aquaculture.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"93 ","pages":"Article 104493"},"PeriodicalIF":4.5,"publicationDate":"2025-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145836704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulation of vegetative propagation by exogenous α-NAA and GA1 in Sargassum hemiphyllum var. chinense 外源α-NAA和GA1对马尾草无性繁殖的调控

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2025-12-23 DOI: 10.1016/j.algal.2025.104497

Yuming Wu , Qiong Wu , Yi Luo , Muhamad Syaifudin , Xianming Tang , Tangcheng Li , Jing Chen , Hong Du

Sargassum hemiphyllum var. chinense is a brown seaweed along the southeastern coast of China, playing a significant ecological role and possessing considerable resource utilization value. α-Naphthaleneacetic acid (α-NAA) and Gibberellin acid 1 (GA1) play an important role in S. hemiphyllum var. chinense holdfast regeneration. However, their regulatory mechanisms remain poorly understood. This study investigated the application of α-NAA and GA1 to promote the rapid regeneration from S. hemiphyllum var. chinense holdfast and further explored its mechanism. Compared to the control, treatment with 1.5 mg·L⁻¹ α-NAA and 1.5 mg·L⁻¹ GA1 significantly reduced the regeneration time by a minimum of four days. The average maximum length of the regenerated leaves was approximately 1.79 times that of the control group, and the leaves exhibited propagules induction capacity. During this developmental process, the transcriptome results showed that upregulated enzymes in both the IPA (Indole-3-Pyruvic Acid) and TAM (Tryptamine) branches of tryptophan metabolism elevate IAA levels, with each pathway acting at distinct stages. Meanwhile, the upregulation of CYP735 A (Cytochrome P450 735 A) significantly increased the levels of 2MeScZ, IP, K, and mT. These cytokinins via the up-regulated CRE1 and B-ARR, ultimately promoting cell division. The GAs (GA13919) showed a very high upregulated level, suggesting the potential importance in the development of Sargassum. Thus, exogenous induction with α-NAA and GA1 can solve the problem of scarce juvenile production from S. hemiphyllum var. chinense holdfasts. This approach promotes rapid juvenile regeneration and improves production efficiency. These findings will contribute to the advancement of artificial propagation in macroalgae.？

马尾藻（Sargassum hemiphyllum var. chinense）是中国东南沿海的一种褐藻，具有重要的生态作用，具有相当的资源利用价值。α-萘乙酸（α-NAA）和赤霉素酸1 （GA1）在赤霉素固结再生中起重要作用。然而，它们的调控机制仍然知之甚少。本研究研究了α-NAA和GA1对赤霉素快速再生的促进作用，并进一步探讨了其作用机制。与对照相比，1.5 mg·L−1 α-NAA和1.5 mg·L−1 GA1处理显著缩短了再生时间至少4天。再生叶片的平均最大长度约为对照组的1.79倍，叶片具有繁殖体诱导能力。在这一发育过程中，转录组研究结果显示，色氨酸代谢的IPA（吲哚-3-丙酮酸）和TAM（色氨酸）分支的上调酶均可提高IAA水平，且每种途径在不同的阶段起作用。同时，上调CYP735 A （Cytochrome P450 735 A）可通过上调CRE1和B-ARR，显著提高2MeScZ、IP、K和mT等细胞分裂素的水平，最终促进细胞分裂。GAs （GA13919）显示出非常高的上调水平，表明其在马尾藻发育中的潜在重要性。由此可见，α-NAA和GA1的外源诱导可以解决南水北流苗木幼鱼产量不足的问题。这种方法促进了幼鱼的快速再生，提高了生产效率。这些发现将有助于推进大型藻人工繁殖。

{"title":"Regulation of vegetative propagation by exogenous α-NAA and GA1 in Sargassum hemiphyllum var. chinense","authors":"Yuming Wu , Qiong Wu , Yi Luo , Muhamad Syaifudin , Xianming Tang , Tangcheng Li , Jing Chen , Hong Du","doi":"10.1016/j.algal.2025.104497","DOIUrl":"10.1016/j.algal.2025.104497","url":null,"abstract":"<div><div><em>Sargassum hemiphyllum</em> var. <em>chinense</em> is a brown seaweed along the southeastern coast of China, playing a significant ecological role and possessing considerable resource utilization value. α-Naphthaleneacetic acid (α-NAA) and Gibberellin acid 1 (GA1) play an important role in <em>S. hemiphyllum</em> var. <em>chinense</em> holdfast regeneration. However, their regulatory mechanisms remain poorly understood. This study investigated the application of α-NAA and GA1 to promote the rapid regeneration from <em>S. hemiphyllum</em> var. <em>chinense</em> holdfast and further explored its mechanism. Compared to the control, treatment with 1.5 mg·L<sup>−1</sup> α-NAA and 1.5 mg·L<sup>−1</sup> GA1 significantly reduced the regeneration time by a minimum of four days. The average maximum length of the regenerated leaves was approximately 1.79 times that of the control group, and the leaves exhibited propagules induction capacity. During this developmental process, the transcriptome results showed that upregulated enzymes in both the IPA (Indole-3-Pyruvic Acid) and TAM (Tryptamine) branches of tryptophan metabolism elevate IAA levels, with each pathway acting at distinct stages. Meanwhile, the upregulation of <em>CYP735 A</em> (Cytochrome P450 735 A) significantly increased the levels of 2MeScZ, IP, K, and mT. These cytokinins via the up-regulated <em>CRE1</em> and <em>B-ARR</em>, ultimately promoting cell division. The GAs (GA13919) showed a very high upregulated level, suggesting the potential importance in the development of <em>Sargassum</em>. Thus, exogenous induction with α-NAA and GA1 can solve the problem of scarce juvenile production from <em>S. hemiphyllum</em> var. <em>chinense</em> holdfasts. This approach promotes rapid juvenile regeneration and improves production efficiency. These findings will contribute to the advancement of artificial propagation in macroalgae.？</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"94 ","pages":"Article 104497"},"PeriodicalIF":4.5,"publicationDate":"2025-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145973858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a scalable design for biofuel production from green macroalgae hydrolysates 从绿色大型藻类水解物生产生物燃料的可扩展设计的发展

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2025-12-22 DOI: 10.1016/j.algal.2025.104487

Hayet Djelal , Alaa Salma , Walaa Sayed , Audrey Cabrol , Maud Benoit , Amance Corat , Abdeltif Amrane , Ronan Pierre

This study investigated the valorization of green macroalgae in ethanol by the yeast Saccharomyces cerevisiae. The proof of concept was conducted with two green macroalgae hydrolysates, namely Ulva rigida and Chaetomorpha linum and the fermentation process was scaled-up from 250 mL and 500 mL shake flasks to a 3 L stirred-tank bioreactor. Firstly, the experiments used a synthetic medium based on algal hydrolysate, examining factors like nitrogen source, inoculum size, and salt content. After 72 h, ethanol yields were similar across the systems: 0.43 g.g⁻¹ in the bioreactor, compared to 0.46 and 0.45 g.g⁻¹ in 250 mL and 500 mL flasks, respectively. Secondly, fermenting green algae hydrolysate was tested, under similar conditions (without aeration and pH adjustment, with nitrogen enrichment, and 1 % v/v inoculum) resulted in an ethanol concentration of 9.05 g.L⁻¹, a yield of 0.44 g.g⁻¹, and 85.86 % efficiency. Thirdly, operating the process at larger scale was carried out on pilot scale of 77 L with Chaetomorpha linum hydrolysate. Glucose was fully consumed in 9 h, and maximum ethanol production (5.61 g.L⁻¹) occurred after 11 h, with a yield of 0.52 g.g⁻¹ which corresponds to the theoretical yield.

本文研究了绿色巨藻在乙醇中的发酵过程。概念验证是用两种绿色大型藻类水解物进行的，即刚性Ulva和毛藻（Chaetomorpha linum），并将发酵过程从250 mL和500 mL摇瓶放大到3 L搅拌槽生物反应器。首先，采用以藻类水解物为基础的合成培养基，考察氮源、接种量、含盐量等因素。72h后，不同体系的乙醇产量相似：生物反应器中的乙醇产量为0.43 g g−1，而250 mL和500 mL烧瓶中的乙醇产量分别为0.46 g g−1和0.45 g g−1。其次，对绿藻水解液进行发酵，在相同的条件下（不曝气，不调节pH，富氮，接种量为1% v/v），乙醇浓度为9.05 g。L−1，产率为0.44 g.g−1，效率为85.86%。再次，以毛藻水解液为原料，在77 L的中试规模上进行了该工艺的大规模操作。葡萄糖在9 h内完全消耗，最大乙醇产量为5.61 g。L−1)在11 h后发生，产率为0.52 g.g−1，符合理论产率。

{"title":"Development of a scalable design for biofuel production from green macroalgae hydrolysates","authors":"Hayet Djelal , Alaa Salma , Walaa Sayed , Audrey Cabrol , Maud Benoit , Amance Corat , Abdeltif Amrane , Ronan Pierre","doi":"10.1016/j.algal.2025.104487","DOIUrl":"10.1016/j.algal.2025.104487","url":null,"abstract":"<div><div>This study investigated the valorization of green macroalgae in ethanol by the yeast <em>Saccharomyces cerevisiae</em>. The proof of concept was conducted with two green macroalgae hydrolysates, namely <em>Ulva rigida and Chaetomorpha linum</em> and the fermentation process was scaled-up from 250 mL and 500 mL shake flasks to a 3 L stirred-tank bioreactor. Firstly, the experiments used a synthetic medium based on algal hydrolysate, examining factors like nitrogen source, inoculum size, and salt content. After 72 h, ethanol yields were similar across the systems: 0.43 g.g<sup>−1</sup> in the bioreactor, compared to 0.46 and 0.45 g.g<sup>−1</sup> in 250 mL and 500 mL flasks, respectively. Secondly, fermenting green algae hydrolysate was tested, under similar conditions (without aeration and pH adjustment, with nitrogen enrichment, and 1 % <em>v</em>/v inoculum) resulted in an ethanol concentration of 9.05 g.L<sup>−1</sup>, a yield of 0.44 g.g<sup>−1</sup>, and 85.86 % efficiency. Thirdly, operating the process at larger scale was carried out on pilot scale of 77 L with <em>Chaetomorpha linum</em> hydrolysate. Glucose was fully consumed in 9 h, and maximum ethanol production (5.61 g.L<sup>−1</sup>) occurred after 11 h, with a yield of 0.52 g.g<sup>−1</sup> which corresponds to the theoretical yield.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"94 ","pages":"Article 104487"},"PeriodicalIF":4.5,"publicationDate":"2025-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145923282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insights into leveraging alga–bacterium cooperation for dairy wastewater valorization 利用藻类-细菌合作实现乳制品废水增值的见解

IF 4.5 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2025-12-22 DOI: 10.1016/j.algal.2025.104481

Caterina Isabel Manzano-Puga , Nuria Membrives-Barea , Aitor Gómez-Osuna , María Jesús Torres , Alexandra Dubini , David González-Ballester

The integration of microalgae-bacteria consortia into wastewater treatment offers a promising strategy for sustainable biomass production and bioremediation. This study investigates the performance of Chlamydomonas reinhardtii in consortium with Serratia liquefaciens DWW128, isolated from raw dairy wastewater (DWW). The consortium, and respective monocultures were cultivated in both untreated, raw DWW and sterile, simulated DWW (sDWW). C. reinhardtii alone exhibited negligible growth in sDWW, while the native microbial community of raw DWW supported its growth (35.2 μg·mL⁻¹ chlorophyll and 1.6 g·L⁻¹ of biomass). However, native microorganisms also negatively affect the alga by limiting its maximum growth compared to cocultures with S. liquefaciens in raw DWW (53.2 μg·mL⁻¹ chlorophyll, 3.1 g·L⁻¹ biomass) and in sDWW (90 μg·mL⁻¹ chlorophyll, 7.1 g·L⁻¹ biomass). The enhancement of algal growth is more pronounced under aerobic conditions, and the supply of ammonium through bacterial proteolytic activity, and possibly also acetic acid, are key factors for algal growth. Cocultures can reduce up to 57.6 % of the bacterial CO₂ emissions. In contrast, S. liquefaciens did not depend on C. reinhardtii to grow successfully in DWW and can produce a substantial amount of bioH₂ (200.7 mL·L⁻¹) in 48 h. However, the presence of the alga enhances the bacterium's viability and persistence. This study provides valuable insights into harnessing the metabolic specialization of diverse microalgal and bacterial species to design tailored consortia capable of exploiting the broad spectrum of nutrient sources in wastewater, enabling more robust and effective biotechnological applications.

Raw Dataset repository: 10.17632/39ckdr8w2n.1

将微藻-细菌联合体整合到废水处理中为可持续生物质生产和生物修复提供了一种有前途的策略。本研究研究了莱茵衣单胞菌与液化沙雷氏菌DWW128联合处理乳废水的性能。在未经处理的原始DWW和无菌的模拟DWW （sDWW）中培养财团和各自的单培养物。单独的莱因哈蒂草（C. reinhardtii）在sDWW中的生长可以忽略不计，而原始DWW的原生微生物群落支持其生长（35.2 μg·mL−1叶绿素和1.6 g·L−1生物量）。然而，与与S.液化菌共培养相比，原生微生物也会对藻类产生负面影响，在原DWW （53.2 μg·mL−1叶绿素，3.1 g·L−1生物量）和sDWW （90 μg·mL−1叶绿素，7.1 g·L−1生物量）中限制藻类的最大生长。在好氧条件下，藻类生长的增强更为明显，通过细菌蛋白水解活性提供的铵，可能还有乙酸，是藻类生长的关键因素。共培养可以减少高达57.6%的细菌二氧化碳排放量。相比之下，液化链球菌不依赖赖因哈氏c菌在DWW中成功生长，并且在48 h内可以产生大量的bioH2 （200.7 mL·L−1）。然而，藻类的存在增强了细菌的生存能力和持久性。这项研究为利用不同微藻和细菌物种的代谢专一性来设计量身定制的联盟提供了有价值的见解，这些联盟能够利用废水中广泛的营养来源，从而实现更强大和有效的生物技术应用。原始数据库：10.17632/39ckdr8w2n.1

{"title":"Insights into leveraging alga–bacterium cooperation for dairy wastewater valorization","authors":"Caterina Isabel Manzano-Puga , Nuria Membrives-Barea , Aitor Gómez-Osuna , María Jesús Torres , Alexandra Dubini , David González-Ballester","doi":"10.1016/j.algal.2025.104481","DOIUrl":"10.1016/j.algal.2025.104481","url":null,"abstract":"<div><div>The integration of microalgae-bacteria consortia into wastewater treatment offers a promising strategy for sustainable biomass production and bioremediation. This study investigates the performance of <em>Chlamydomonas reinhardtii</em> in consortium with <em>Serratia liquefaciens</em> DWW128, isolated from raw dairy wastewater (DWW). The consortium, and respective monocultures were cultivated in both untreated, raw DWW and sterile, simulated DWW (sDWW). <em>C. reinhardtii</em> alone exhibited negligible growth in sDWW, while the native microbial community of raw DWW supported its growth (35.2 μg·mL<sup>−1</sup> chlorophyll and 1.6 g·L<sup>−1</sup> of biomass). However, native microorganisms also negatively affect the alga by limiting its maximum growth compared to cocultures with <em>S. liquefaciens</em> in raw DWW (53.2 μg·mL<sup>−1</sup> chlorophyll, 3.1 g·L<sup>−1</sup> biomass) and in sDWW (90 μg·mL<sup>−1</sup> chlorophyll, 7.1 g·L<sup>−1</sup> biomass). The enhancement of algal growth is more pronounced under aerobic conditions, and the supply of ammonium through bacterial proteolytic activity, and possibly also acetic acid, are key factors for algal growth. Cocultures can reduce up to 57.6 % of the bacterial CO<sub>2</sub> emissions. In contrast, <em>S. liquefaciens</em> did not depend on <em>C. reinhardtii</em> to grow successfully in DWW and can produce a substantial amount of bioH<sub>2</sub> (200.7 mL·L<sup>−1</sup>) in 48 h. However, the presence of the alga enhances the bacterium's viability and persistence. This study provides valuable insights into harnessing the metabolic specialization of diverse microalgal and bacterial species to design tailored consortia capable of exploiting the broad spectrum of nutrient sources in wastewater, enabling more robust and effective biotechnological applications.</div><div><strong>Raw Dataset repository</strong>: <span><span>10.17632/39ckdr8w2n.1</span><svg><path></path></svg></span></div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"94 ","pages":"Article 104481"},"PeriodicalIF":4.5,"publicationDate":"2025-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145923305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0