Algal Research-Biomass Biofuels and Bioproducts最新文献

{"title":"Algal-bacterial consortia-derived cyanophycin reprograms rice seed metabolism for enhanced abiotic stress resilience","authors":"Zirui Zhou ,&nbsp;Duoduo Chen ,&nbsp;Naif Abdullah Al-Dhabi ,&nbsp;Caicheng Long ,&nbsp;Taiping Qing ,&nbsp;Bo Feng ,&nbsp;Wangwang Tang ,&nbsp;Peng Zhang","doi":"10.1016/j.algal.2025.104491","DOIUrl":"10.1016/j.algal.2025.104491","url":null,"abstract":"<div><div>The utilization of algal-bacterial biomass for sustainable, low-carbon agricultural development represents a promising strategy for the efficient recycling of resources. This study explored the effect of cyanophycin granule polypeptide (CGP) extract on rice seed resistance and underlying mechanisms under various stress conditions. Results revealed that CGP notably improved the germination rate and growth of rice seeds subjected to drought (20 % polyethylene glycol), heavy metal contamination (3 mg/L Cd²⁺), and salinity (50 mM NaCl). After 48 h, seeds treated with 30 mg/L CGP exhibited a germination rate of 90 ± 2 %, significantly outperforming other treatments (<em>P</em> &lt; 0.05). CGP treatment also elevated soluble sugar and protein levels, boosted α-amylase activity, and ensured adequate energy and nutrition for seed germination. Additionally, CGP enhanced seed antioxidant capacity, significantly increasing the activities of superoxide dismutase, peroxidase, and catalase, while reducing malondialdehyde content and mitigating membrane lipid peroxidation. CGP further promoted proline accumulation, improving the water retention capacity of rice seeds. Metabolomic analysis indicated that CGP induced metabolic reprogramming, driving the synthesis of stress-related metabolites such as phenolic compounds and salicylic acid, thus strengthening the seed's immune response. Notably, the ‘stress memory’ effects persisted into the seedling stage. This study introduces a novel approach for employing CGP as a plant biostimulant to enhance crop resilience under abiotic stress.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"93 ","pages":"Article 104491"},"PeriodicalIF":4.5,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145880218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing Sargassum inundation biomass: Biobased products versus energy valorisation","authors":"Karla Dussan ,&nbsp;Paulina S. Birgersson ,&nbsp;Stefania Luzzi ,&nbsp;Olav A. Aarstad ,&nbsp;Esther Cobussen-Pool ,&nbsp;Tim Koster ,&nbsp;Heather E. Wray ,&nbsp;Finn L. Aachmann","doi":"10.1016/j.algal.2025.104488","DOIUrl":"10.1016/j.algal.2025.104488","url":null,"abstract":"<div><div>Different routes for extraction of polysaccharides (fucoidan, alginate) and biomethane production were evaluated for the valorisation of <em>Sargassum</em> spp. biomass. Modest yields of alginate (145 g/kg dry biomass) and fucoidan (43 g/kg dry biomass) were achieved through sequential low temperature acidic aqueous treatment and alkaline extraction. The crude extracts showed low molecular weights (45.2 and 79.5 kDa, respectively) and had brown colour suggesting the presence of polyphenols. High temperature aqueous treatment increased fucoidan extraction efficiency, as indicated by higher fucose content in hydrolysates; however, the extract had low molecular weight (&lt;10 kDa) leading to low crude fucoidan yield (27 g/kg dry biomass) and low fucose content. The alginate extraction yield and composition were negatively affected by the higher temperature treatment. Anaerobic digestion (AD) tests of untreated and treated fractions of <em>Sargassum</em> biomass showed that high temperature aqueous treatment can increase the biomethane production potential by up to 5 times (from 23.5 to 153.3 m³/ton volatile solids). The most energy-rich and AD suitable fraction from the high temperature aqueous treatment was the hydrolysate fraction, containing fucoidan and other solubilised organics, while the residual <em>Sargassum</em> biomass displayed relatively low biomethane potential (79.7 m³/ton volatile solids). A simplified economic analysis suggests that using <em>Sargassum</em> biomass in the Caribbean can be profitable (ROI between 12 and 28 %), and products such as fucoidan and alginate may enhance economic viability. However, the study shows the sensitivity of valorisation concepts to both product prices and yields, in particular the risk associated with poor product quality.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"93 ","pages":"Article 104488"},"PeriodicalIF":4.5,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145920655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytohormone-regulated algal-bacterial-fungal tri-culture for efficient removal of antibiotics and antibiotic resistance genes in wastewater","authors":"Chunzhi Zhao ,&nbsp;Rui Feng ,&nbsp;Zhengfang Wang ,&nbsp;Jing Wei ,&nbsp;Yongjun Zhao ,&nbsp;Shoubing Wang","doi":"10.1016/j.algal.2025.104508","DOIUrl":"10.1016/j.algal.2025.104508","url":null,"abstract":"<div><div>Aquaculture wastewater frequently contains residual multi-class antibiotics and antibiotic resistance genes (ARGs), which pose severe ecological risks and remain challenging for traditional treatment technologies to address effectively. Phytohormones were selected as regulatory factors because of their well-documented role in modulating microbial growth, photosynthetic activity, and metabolic synergy—key traits for enhancing the pollutant removal capacity of symbiotic algal-bacterial-fungal systems. This study investigated the effects of four phytohormones, including gibberellic acid (GA3), indole-3-acetic acid (IAA), strigolactone analog GR24, and abscisic acid (ABA), on the removal of six target antibiotics and corresponding ARGs using four algal-bacterial-fungal symbiotic systems. The tested systems included the monoculture of <em>Scenedesmus obliquus</em>, algal-bacterial co-culture, algal-fungal co-culture, and algal-bacterial-fungal tri-culture of <em>S. obliquus</em>-<em>Pseudomonas</em> sp.-<em>Aspergillus niger</em>. Results showed that the tri-culture system treated with 50 mg L⁻¹ GA3 achieved the highest removal efficiency for all pollutants. For antibiotics, tetracyclines (TET) including Tetracycline (TET) and Chlortetracycline (CTC) had the highest removal rates at 99.81 % and 97.34 %, followed by quinolones (QNs) including Ciprofloxacin (CIP) and Enrofloxacin (ENR) at 81.36 % and 78.52 %, and sulfonamides (SAs) including Sulfadiazine (SDZ) and Sulfamethoxazole (SMX) at 71.81 % and 68.53 %. For ARGs, plasmid-borne genes <em>sul</em>1 and <em>sul</em>2 exhibited the most significant reduction (3.67 lg copies and 2.87 lg copies, respectively), while tetracycline resistance genes (<em>tet</em>A, <em>tet</em>B) and quinolone resistance genes (<em>qnr</em>A, <em>gyr</em>A) showed relatively smaller reductions (2.51, 1.41 lg copies and 1.16, 1.31 lg copies, respectively). Mechanistically, GA3 enhanced the tri-culture's performance by improving photosynthetic efficiency. This improvement was reflected in increased maximum photochemical efficiency of PSII (<em>F</em>_V/<em>F</em>_M = 0.89) and photosynthetic performance index (<em>PI</em>_ABS = 7.51). This study demonstrated that the GA3-regulated <em>S. obliquus</em>-<em>Pseudomonas</em> sp.-<em>Aspergillus niger</em> tri-culture system offers a novel and sustainable strategy for aquaculture wastewater treatment.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"93 ","pages":"Article 104508"},"PeriodicalIF":4.5,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145880220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Larvicidal activity, oviposition deterrence, enzymatic test and docking of essential oil of Laurencia dendroidea (Rhodophyta, Ceramiales) for control of the mosquito Aedes aegypti L.","authors":"Suyana Karolyne Lino da Rocha ,&nbsp;Kamila de Andrade Dutra ,&nbsp;Bheathiz Nunes de Lima ,&nbsp;Camila Soledade de Lira Pimentel ,&nbsp;Ana Carla da Silva ,&nbsp;Priscila Soares da Silva ,&nbsp;Fabio Henrique Galdino dos Santos ,&nbsp;Júlio César Ribeiro de Oliveira Farias de Aguiar ,&nbsp;Gilson José da Silva Gomes Vieira ,&nbsp;Juliane Bernardi Vasconcelos ,&nbsp;Ana Patrícia Silva de Oliveira ,&nbsp;Thiago Henrique Napoleão ,&nbsp;Patrícia Maria Guedes Paiva ,&nbsp;Emanuelly Karla Araújo Padilha ,&nbsp;Edeildo Ferreira da Silva-Júnior ,&nbsp;Daniela Maria do Amaral Ferraz Navarro","doi":"10.1016/j.algal.2025.104498","DOIUrl":"10.1016/j.algal.2025.104498","url":null,"abstract":"<div><div>The objectives of the present study were to investigate the insecticidal and oviposition activity of the oil as well as the mode of action through experimental enzymatic and docking studies. Larvicidal (LC₅₀) and oviposition assays were performed with the <em>Aedes aegypti</em> mosquito using the essential oil (19.1 mg.mL⁻¹), the hydrolate (42.8 % <em>v</em>/v) and the aqueous extract (49.7 % v/v). The hydrodistillation products of L. <em>dendroidea</em> also showed significant oviposition deterrent effects (<em>p</em> &lt; 0.05) for the essential oil at 15 and 20 mg.mL⁻¹, hydrolate at 20 % <em>v</em>/v and aqueous extract at 50 % v/v. Enzymatic tests demonstrated that the essential oil inhibits amylase in the insect, indicating that this may be the mode of action by which the oil kills the larvae. Docking tests were performed with the major compounds and amylase to determine the types of interaction between these molecules in the oil and the enzyme. The major compound of the oil, representing approximately 90 % of its composition, showed in silico affinity with the same enzyme in docking studies, suggesting that it is a potential contributor to the observed activity. This is the first report in the literature on the larvicidal and oviposition deterrent activity of L. <em>dendroidea</em> essential oil for the control of <em>Ae. aegypti</em>.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"93 ","pages":"Article 104498"},"PeriodicalIF":4.5,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145880219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advanced feature engineering and Gradient Boosting optimization for predicting Limnospira platensis growth dynamics under mixotrophic conditions using agro-industrial byproducts","authors":"Bartolomeo Cosenza ,&nbsp;Riccardo Allodi ,&nbsp;Luca Usai ,&nbsp;Riccardo Minardi ,&nbsp;Alessandro Cosenza ,&nbsp;Robinson Soto-Ramirez ,&nbsp;Alessandro Concas ,&nbsp;Giovanni Antonio Lutzu","doi":"10.1016/j.algal.2025.104445","DOIUrl":"10.1016/j.algal.2025.104445","url":null,"abstract":"<div><div>This research investigates advanced computational modeling strategies for predicting <em>Limnospira platensis</em> growth dynamics under mixotrophic cultivation with the use of agro-industrial processing residues. The cultivation approach combines photosynthetic carbon fixation with organic carbon utilization, potentially enhancing both biomass yields and valuable metabolite production. By implementing sophisticated data analysis frameworks, this work aims to optimize cultivation parameters while advancing waste-to-resource conversion principles. Three effluents, cheese whey (scotta), tomato extract, and artichoke extract, were tested at multiple concentrations and compared with a control medium. Experimental results revealed that whey-based substrates achieved the highest biomass productivity, reaching up to 6.1 g L^{<strong>−1</strong>}, while artichoke extract media resulted in lower yields (approximately 3.0 g L^{<strong>−1</strong>}). To optimize predictive performance, Gradient Boosting algorithms were tuned through Bayesian hyperparameter optimization, demonstrating strong predictive capabilities for biomass accumulation, with an overall determination coefficient of R² = 0.97 and a root mean square error of 0.19 in cross-validation. Feature importance analysis identified temporal parameters and conversion efficiency as predominant factors governing biomass accumulation. These findings demonstrate that the integration of a data-driven modeling framework and circular bioeconomy strategies can effectively transform agro-industrial waste streams into valuable cost-efficient substrates, reducing waste and supporting sustainable production of high-value biomolecules for food, pharmaceutical, and nutraceutical applications.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"93 ","pages":"Article 104445"},"PeriodicalIF":4.5,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145880221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unravelling the hidden complexities of R-phycoerythrins with a 3D-analytical chemistry approach","authors":"Andres Niroda ,&nbsp;Rando Tuvikene ,&nbsp;Michael Landreh ,&nbsp;Mihkel Saluri","doi":"10.1016/j.algal.2025.104494","DOIUrl":"10.1016/j.algal.2025.104494","url":null,"abstract":"<div><div>R-phycoerythrin (R-PE) is a vivid, proteinaceous pigment found in nature that holds promise for applications in biotechnology, cosmetics, and the food industry. Because recombinant production of the intact α-, β-, and γ-subunit R-PE complex remains challenging, it is purified from naturally sourced red algae. Notably, R-PE properties vary among algal species, offering opportunities to select optimal sources for specific applications. Here, we purified R-PE from ten red algal species and characterised them using a multi-dimensional strategy integrating UV–Vis absorption spectroscopy, fluorimetry, HPLC, and native mass spectrometry to pinpoint interspecies differences.</div><div>In a comparative analysis of ten red algae species, R-PE from <em>Ceramium tenuicorne</em> displayed the highest fluorescence intensity, likely due to a protein environment that maintains chromophores in an elongated conformation, promoting efficient energy transfer. Measurements also showed that <em>Furcellaria lumbricalis</em> and <em>Rhodomela confervoides</em> isolates exhibited 4–5-times lower fluorescence intensity at similar absorbances, suggesting they are more suitable for food colouring than as fluorescent probes. Using RP-HPLC with a PDA detector, a blue-shift in the α-subunit absorbance was observed for <em>F. lumbricalis</em> and the presence of two α-subunit isoforms in <em>Neopyropia yezoensis</em> were identified, indicating greater interspecies heterogeneity than previously recognised. In contrast, absorbances of β- and γ-subunits displayed remarkable consistency. Fluorescence intensity of intact R-PE was positively associated with γ-subunit hydrophilicity and absorbance, underscoring the crucial role of the central γ-subunits in R-PE brightness.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"93 ","pages":"Article 104494"},"PeriodicalIF":4.5,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145920656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Branch-point engineering of carotenoid pathways reshapes ketocarotenoid profiles and photosynthetic performance in Chlamydomonas reinhardtii","authors":"Junhwan Jang ,&nbsp;Byumjune Park ,&nbsp;Changbeom Cho ,&nbsp;Seunghye Park ,&nbsp;EonSeon Jin","doi":"10.1016/j.algal.2025.104506","DOIUrl":"10.1016/j.algal.2025.104506","url":null,"abstract":"<div><div>Carotenoid biosynthesis in <em>Chlamydomonas reinhardtii</em> is tightly partitioned into α- and β-branches, yielding lutein and violaxanthin but lacking ketocarotenoids under standard conditions. Previous engineering efforts have focused mainly on <em>β-carotene ketolase</em> (<em>BKT</em>) overexpression to enhance astaxanthin accumulation, but the interplay between pathway branch points and photosynthetic performance remains poorly understood. Here we combined targeted knockouts of <em>β-carotenoid hydroxylase</em> (<em>CHYB</em>) and <em>lycopene ε-cyclase</em> (<em>LCYE</em>) with <em>BKT</em> overexpression to reprogram metabolic flux toward non-hydroxylated ketocarotenoids. <em>BKT</em> expression in the wild-type background (B7) resulted in mixed profiles of canthaxanthin (1.28 ± 0.16 mg L⁻¹), adonirubin (2.19 ± 0.09 mg L⁻¹), astaxanthin (2.39 ± 0.15 mg L⁻¹), whereas <em>CHYB</em> disruption (dc_B10) abolished astaxanthin production and redirected flux predominantly to canthaxanthin (4.44 ± 0.01 mg L⁻¹; 3.47-fold vs. B7). Additional <em>LCYE</em> removal (dcl_B8) eliminated α-branch products and yielded simplified pigment profiles enriched in non-hydroxylated ketocarotenoids. Despite the lack of astaxanthin, dcl_B8 exhibited partial recovery of the chlorophyll content (2.87 ± 0.15 vs. 1.29 ± 0.24 pg cell⁻¹ in dc_B10), PSII efficiency (Fv/Fm = 0.49 vs. 0.19 in dc_B10), and non-photochemical quenching (NPQ, 0.15 vs. 0.016 in dc_B10), while achieving the highest oxygen evolution under saturating light and elevated half-saturation irradiance conditions. These results demonstrate that photosynthetic impairment in ketocarotenoid-accumulating strains is not dictated by the total pigment content but by the specific configuration of carotenoids supporting PSII stability and photoprotection.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"94 ","pages":"Article 104506"},"PeriodicalIF":4.5,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146023287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oligo-carrageenan kappa, oligo-ulvans and carrageenan kappa differentially increase the level of hormones involved in growth and defense against biotic and abiotic stresses in Arabidopsis thaliana","authors":"Rodrigo A. Contreras ,&nbsp;Alberto González ,&nbsp;Daniel Laporte ,&nbsp;Alejandra Moenne","doi":"10.1016/j.algal.2025.104507","DOIUrl":"10.1016/j.algal.2025.104507","url":null,"abstract":"<div><div><em>Arabidopsis thaliana</em> plants were treated on leaves with an aqueous solution of oligo-carrageenan kappa (OCK), oligo-ulvans (OU) and carrageenan kappa (CK) at a concentration of 1 mg mL⁻¹, five times, every two days, 15 d after germination (ag) and samples were collected at 20 d and 30 d ag. Phytohormones involved in the stimulation of growth such as the auxins indole-3-acetic acid (IAA) and indole-3-butyric acid (IBA), the gibberellins A3, A4 and A7, the cytokinins isopentenyl adenine (iP) and trans-zeatin (tZ), and the brassinosteroids castasterone (CAS) and epibrassinolide (eBL) were detected. Results indicate that OCK, OU and CK increased IAA level, mainly OCK; OCK increased GA3 level, OCK and OU increased GA4 and GA7 levels, but not CK; OCK increased iP level and OCK and OU increased in tZ level, but not CK, and OCK and OU increased eBL level, but not CK. Thus, OCK and OU increased growth-promoting hormones such as AUXs, GAs, CKs and BRs levels in <em>A. thaliana</em>. On the other hand, hormones involved in biotic and abiotic stress tolerance such as abscisic acid (ABA), 1-aminocyclopropane-1-carboxylic (ACC), castasterone (CAS), jasmonates and salicylates were detected. OCK increased ABA level; OCK and OU increased ACC levels and OU increased CAS level; OCK increased JA level, OCK and OU increased JA-ile level, OCK, OU and CK increased MeJA level; OCK, OU and CK increased SA level and OCK, OU and CK increased MeSA level. Thus, OCK increased ABA level suggesting that tolerance against drought may be enhanced; OCK and OU increased ACC, JA, JA-ile, MeJA, SA and MeSA suggesting that defense against pathogens and abiotic stress may also be increased. Therefore, OCK, OU and CK differentially stimulate growth and defense against biotic and abiotic stresses in <em>A. thaliana</em>.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"94 ","pages":"Article 104507"},"PeriodicalIF":4.5,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145923306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Revealing the reinforcement mechanisms of compound bacterial agents to the blackwater treatment performance of microalgae-fungi-sludge consortia through metagenomics","authors":"Zeyuan Wang ,&nbsp;Guanqiu Ma ,&nbsp;Ganlin Hu ,&nbsp;Zile Jiao ,&nbsp;Yu Hong","doi":"10.1016/j.algal.2025.104514","DOIUrl":"10.1016/j.algal.2025.104514","url":null,"abstract":"<div><div>This study utilized a novel microbial system, that is, microalgae-fungi-sludge consortia (MFSC), to treat blackwater (BW). In addition, this study also explored the improvement effects of compound bacterial agents (CBA) on the treatment performance of MFSC. CBA efficiently improved the settleability (sludge volume index (SVI₅): 48.31 mL/g) of MFSC. MFSC exhibited good nutrient removal performance. CBA further enhanced the BW treatment ability of MFSC, the total nitrogen (TN) and NH₄⁺-N removal efficiencies separately increased by 6.38 % and 6.33 %. Metagenomics analysis indicated that CBA increased the abundance of critical functional microorganisms such as <em>Micropruina</em> and <em>Rhodanobacter</em>. Besides community regulation, CBA ameliorated the physiological state and BW treatment ability of MFSC by adjusting nitrogen metabolism, quorum sensing, carbohydrate metabolism, and amino acid metabolism. This study provides a novel microbial system and efficient reinforcement strategy for treating BW, as well as providing novel insights into the comprehensive mechanisms of CBA-enhanced BW treatment.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"94 ","pages":"Article 104514"},"PeriodicalIF":4.5,"publicationDate":"2025-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145883761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improvement of molecular tools for the model diatom Thalassiosira pseudonana","authors":"Annika Messemer ,&nbsp;Per Winge ,&nbsp;Tore Brembu","doi":"10.1016/j.algal.2025.104509","DOIUrl":"10.1016/j.algal.2025.104509","url":null,"abstract":"<div><div>The centric diatom <em>Thalassiosira pseudonana</em> is a model organism for studies of diatom biology, especially the biomineralization of its frustule. Methods for genetic transformation of this species have primarily been limited to biolistic transformation and conjugation. Biolistic methods present significant disadvantages in terms of accessibility and handling, while conjugation requires a diatom-specific backbone and a helper plasmid. In this study an efficient and straightforward transformation method using electroporation was established. Two electroporation systems were investigated, and reliable protocols were implemented. Transformation efficiency, with an average of 88 colony-forming cells and reaching up to 1324 per reaction, is more than sufficient for genome editing or other approaches and presenting up to a 3-fold improvement compared to biolistic transformation. A major advantage of this method is the possibility of genomic integration of any plasmid, making the usage of diatom specific vectors redundant while ensuring stable expression over time. Furthermore, a new small, strong and reliable native promoter-terminator pair was explored using the reporter gene <em>mNeonGreen</em>. Fluorescence microscopy and flow cytometry showed stable mNeonGreen fluorescence over time. Expression of target genes was further enhanced by inserting the small native <em>LHCF9</em> intron. Its unique positioning within the start codon of the corresponding gene makes it universally usable. Combining the <em>LHCF9</em> intron with its native promoter strongly increased reporter gene expression; this was confirmed on protein level using a fluorescence reporter. In summary, these new tools can be helpful accessing <em>T. pseudonana</em> for genome editing as well as for improving production of valuable compounds.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"94 ","pages":"Article 104509"},"PeriodicalIF":4.5,"publicationDate":"2025-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145923281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}