Algal Research-Biomass Biofuels and Bioproducts最新文献_第8页

Molecular insights into the enhanced growth of cyanobacteria by adaptive laboratory evolution in wastewater environments 通过适应性实验室进化提高蓝藻在废水环境中生长的分子见解

IF 4.6 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2024-10-01 DOI: 10.1016/j.algal.2024.103724

Lang Hu , Xinyi Fang , Lelei Wen , Haixin Zhang , Baoyu Peng , Changchun Li

The expansion of population leads to an increase in nutrient-rich wastewater, posing a threat to the ecosystem. The cultivation of economically beneficial cyanobacteria consumes amounts of freshwater, exacerbating the depletion of freshwater resources. This study investigates the potential of utilizing adaptive laboratory evolution (ALE) to enhance the growth performance of Synechocystis sp. PCC 6803, a model cyanobacterium, in wastewater. After 374 days of ALE, a strain designated as WW was successfully evolved. When cultivated in wastewater, WW exhibited a specific growth rate of 0.317 per day and achieved a dry weight of 0.693 g/L by the 13th day, outperforming the wild type. WW achieved removal efficiencies of 35.55 % for total nitrogen and 60.95 % for total phosphorus in the wastewater. RNA sequencing and photosynthetic measurements revealed that enhanced photosynthetic capacity in the WW contributes to its superior growth performance. The lipid content in WW was 16.19 %, with a notable increase in the proportion of polyunsaturated fatty acids. The shift in fatty acid composition has a consequential impact on biodiesel index, including oxidation stability and saponification number. This study not only demonstrates the effectiveness of ALE in enhancing the growth of cyanobacteria in wastewater for biofuel production, but also offers significant insights into the molecular mechanisms that drive this improved performance.

人口膨胀导致富含营养物质的废水增加，对生态系统构成威胁。对经济有益的蓝藻的培育需要消耗大量淡水，加剧了淡水资源的枯竭。本研究探讨了利用实验室适应性进化（ALE）提高蓝藻模型 Synechocystis sp.经过 374 天的适应性实验室进化，一株名为 WW 的菌株成功进化。在废水中培养时，WW 的特定生长率为每天 0.317，到第 13 天时干重达到 0.693 克/升，优于野生型。WW 对废水中总氮和总磷的去除率分别为 35.55% 和 60.95%。RNA 测序和光合作用测量结果表明，WW光合作用能力的增强是其生长性能优越的原因之一。WW 中的脂质含量为 16.19%，多不饱和脂肪酸的比例明显增加。脂肪酸组成的变化会对生物柴油的氧化稳定性和皂化度等指标产生影响。这项研究不仅证明了 ALE 在提高废水中蓝藻的生长以生产生物燃料方面的有效性，而且还为我们深入了解驱动这种性能改善的分子机制提供了重要依据。

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引用次数: 0

Evaluation of optimal culture conditions for marine benthic diatom Halamphora coffeaeformis growth and its biochemical properties 评估海洋底栖硅藻 Halamphora coffeaeformis 生长的最佳培养条件及其生化特性

IF 4.6 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2024-09-26 DOI: 10.1016/j.algal.2024.103720

Ninie Diana Baharuddin , Norulhuda Mohamed Ramli , Nurarina Ayuni Ghazali , Hui Teng Tan , Yam Sim Khaw , I. Natrah

Halamphora coffeaeformis is a benthic diatom that exhibits a high-value biochemical composition and can be harnessed for various applications particularly in aquaculture. Despite its potential, significant challenges persist in the optimization of cultivation parameters and scaling up to mass cultivation level. Thus, this study aimed to identify the most suitable substrate, as well as the optimal light intensity, photoperiod, salinity, and pH for the growth of H. coffeaeformis. The biochemical composition of mass-cultured H. coffeaeformis was also analyzed. The findings revealed that polyvinyl chloride (PVC) substrates substantially outperformed bamboo by yielding significantly higher (p < 0.05) growth performance. The maximum specific growth rate (SGR) of H. coffeaeformis was recorded under the following conditions: the light intensity of 120 μmol m⁻² s⁻¹ with an 18: 6 h light/dark cycle (0.200 day⁻¹), the salinity of 30 ppt (0.161 day⁻¹) and the pH of 9 (0.409 day⁻¹). Under these optimized conditions, the mass culture of H. coffeaeformis at 100 L exhibited the highest ash content (38.47 ± 0.17 % dry weight (DW)), followed by crude protein (26.26 ± 0.56 % DW) containing high-quality amino acids, carbohydrates (20.96 ± 0.81 % DW) and crude lipids (4.46 ± 0.56 % DW). The lipid profile was particularly notable for its high saturated fatty acid (SFA) content and sufficient levels of polyunsaturated fatty acids (PUFA), while maintaining the lowest fiber content (0.07 ± 0.01 % DW). These findings underscore the potential of H. coffeaeformis for large-scale aquaculture applications and highlight the importance of fine-tuning cultivation parameters to maximize its biochemical yields.

咖啡硅藻（Halamphora coffeaeformis）是一种底栖硅藻，具有高价值的生化成分，可用于各种用途，尤其是水产养殖。尽管其潜力巨大，但在优化培养参数和扩大大规模培养水平方面仍存在重大挑战。因此，本研究旨在确定最适合 H. coffeaeformis 生长的基质以及最佳光照强度、光周期、盐度和 pH 值。研究还分析了大量培养的 H. coffeaeformis 的生化成分。研究结果表明，聚氯乙烯（PVC）基质的生长性能明显优于竹子（p < 0.05）。咖啡藻的最大特定生长率（SGR）是在以下条件下记录的：光照强度为 120 μmol m-2 s-1，光暗周期为 18: 6 h（0.200 天-1），盐度为 30 ppt（0.161 天-1），pH 值为 9（0.409 天-1）。在这些优化条件下，大量培养 100 升的咖啡藻灰分含量最高（38.47 ± 0.17 % 干重），其次是含有优质氨基酸的粗蛋白（26.26 ± 0.56 % 干重）、碳水化合物（20.96 ± 0.81 % 干重）和粗脂（4.46 ± 0.56 % 干重）。脂质的特点是饱和脂肪酸（SFA）含量高，多不饱和脂肪酸（PUFA）含量充足，同时纤维含量最低（0.07 ± 0.01 % DW）。这些发现凸显了咖啡藻在大规模水产养殖应用中的潜力，并强调了微调养殖参数以最大限度提高其生化产量的重要性。

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引用次数: 0

Study on the interventional effects of Chlamydomonas reinhardtii peptides on chronic unpredictable mild stress-induced depressive-like model mice through metabolomics and microbiota 通过代谢组学和微生物群研究衣藻多肽对慢性不可预测轻度应激诱导的抑郁样模型小鼠的干预作用

IF 4.6 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2024-09-23 DOI: 10.1016/j.algal.2024.103715

Ya Mao , Meiting Liu , Keying Su , Jintao Xie , Wenxia Liu , Lixia Wu , Xuewu Zhang

Depression is a progressive neurodegenerative disease characterized by high prevalence, high suicide rate and high recurrence rate. In this study, the protein from Chlamydomonas reinhardtii was extracted and neutral protease hydrolysate (NPH) was obtained. Its in vitro MAO-A (Monoamine oxidase A) inhibitory activity and in vivo anti-depressive effects in chronic unpredictable mild stress (CUMS) model mice were investigated. The results demonstrated that NPH can improve depressive behaviour in CUMS model mice by elevating neurotransmitter levels and alleviating hippocampal tissue structure damage. The metabolomic analysis of brain and serum samples showed that their common metabolic pathways associated with anti-depressive effects are mainly alanine, aspartate, and glutamate metabolism, glycerophospholipid metabolism, tryptophan metabolism, and caffeine metabolism. Then, the gut microbiota analysis of feces indicated that 8 species with significant changes were associated with anti-depressive effects. Finally, 5 pairs of highly correlated metabolite-bacterium pairs were identified to modulate depressive behaviours. Taken together, the present data suggests that Chlamydomonas reinhardtii-derived hydrolysate could be used for development of functional foods with potential to improve depression.

抑郁症是一种进行性神经退行性疾病，具有高发病率、高自杀率和高复发率的特点。本研究从衣藻中提取蛋白质，得到中性蛋白酶水解物（NPH）。研究了NPH的体外MAO-A（单胺氧化酶A）抑制活性和在慢性不可预知轻度应激（CUMS）模型小鼠体内的抗抑郁作用。结果表明，NPH能通过提高神经递质水平和减轻海马组织结构损伤来改善CUMS模型小鼠的抑郁行为。脑样本和血清样本的代谢组学分析表明，它们与抗抑郁作用相关的常见代谢途径主要是丙氨酸、天门冬氨酸和谷氨酸代谢、甘油磷脂代谢、色氨酸代谢和咖啡因代谢。然后，粪便中的肠道微生物群分析表明，有 8 个物种的显著变化与抗抑郁作用有关。最后，还发现了 5 对高度相关的代谢物-细菌配对，可调节抑郁行为。综上所述，本研究的数据表明，衣藻衍生的水解物可用于开发具有改善抑郁潜能的功能性食品。

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引用次数: 0

Optimizing phlorotannins extraction from Fucus vesiculosus using Box-Behnken design: Unveiling techniques for enhanced antioxidant activity and metabolic enzyme inhibition 利用 Box-Behnken 设计优化从 Fucus vesiculosus 中提取叶绿素：揭示增强抗氧化活性和抑制代谢酶的技术

IF 4.6 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2024-09-22 DOI: 10.1016/j.algal.2024.103723

Hazem Golshany , Aya Kamal , Qun Yu , Liuping Fan

Phlorotannins, phenolic compounds found in brown macroalgae, exhibit potential bioactive effects. This study evaluated the extraction of phlorotannins from Fucus vesiculosus using conventional and ultrasound-assisted extraction (UAE) methods, focusing on solvent-material ratio, solvent concentration, extraction time, and sonication power for UAE. The experiments, designed using a Box-Behnken design, measured total phlorotannin content (TPhC), DPPH, FRAP, and ABTS. The conventional method, under optimal conditions, produced TPhC, DPPH, FRAP, and ABTS values of 512.25 ± 14.26 mg PGE/100 g, 29.70 ± 1.40 mg TE/100 g, 589.57 ± 15.06 mg TE/100 g, and 332.73 ± 31.03 mg TE/100 g, respectively. The UAE method, under its optimal conditions, yielded higher values: 954.00 ± 33.65 mg PGE/100 g, 146.35 ± 3.30 mg TE/100 g, 1037.61 ± 32.93 mg TE/100 g, and 809.95 ± 46.58 mg TE/100 g. The extracts were tested for inhibitory effects on α-amylase and α-glucosidase, with the purified UAE extract showing greater inhibition of α-amylase (IC₅₀ = 7.06 ± 0.67 μg/mL) compared to acarbose (IC₅₀ = 14.63 ± 0.28 μg/mL). For α-glucosidase, acarbose had higher activity (IC₅₀ = 11.54 ± 0.04 μg/mL) than the UAE purified extract (IC₅₀ = 15.04 ± 0.07 μg/mL). UHPLC-qTOF-MS analysis identified various phlorotannin derivatives, with Tetrafucotetraphlorethol (m/z 993) only in the UAE purified extract. This study concludes that purified phlorotannin extract from UAE can potentially regulate α-amylase and α-glucosidase, enzymes linked to metabolic disorders like diabetes and obesity.

绿单宁是一种存在于棕色大型藻类中的酚类化合物，具有潜在的生物活性作用。本研究评估了采用传统和超声辅助萃取（UAE）方法从岩藻中萃取绿单宁的情况，重点关注 UAE 的溶剂-材料比、溶剂浓度、萃取时间和超声功率。实验采用 Box-Behnken 设计，测量了总单宁含量（TPhC）、DPPH、FRAP 和 ABTS。在最佳条件下，传统方法产生的 TPhC、DPPH、FRAP 和 ABTS 值分别为 512.25 ± 14.26 毫克 PGE/100 克、29.70 ± 1.40 毫克 TE/100 克、589.57 ± 15.06 毫克 TE/100 克和 332.73 ± 31.03 毫克 TE/100 克。在最佳条件下，阿联酋方法得到了更高的数值：954.00 ± 33.65 mg PGE/100 g、146.35 ± 3.30 mg TE/100 g、1037.61 ± 32.93 mg TE/100 g 和 809.95 ± 46.58 mg TE/100 g。测试了提取物对α-淀粉酶和α-葡萄糖苷酶的抑制作用，与阿卡波糖（IC50 = 14.63 ± 0.28 μg/mL）相比，纯化的 UAE 提取物对α-淀粉酶的抑制作用更大（IC50 = 7.06 ± 0.67 μg/mL）。对于α-葡萄糖苷酶，阿卡波糖的活性（IC50 = 11.54 ± 0.04 μg/mL）高于阿联酋纯化提取物（IC50 = 15.04 ± 0.07 μg/mL）。超高效液相色谱-qTOF-MS分析鉴定出了多种绿丹宁衍生物，其中只有阿联酋纯化提取物中含有四桉叶油醇（m/z 993）。这项研究的结论是，从阿联酋纯化的叶单宁提取物有可能调节α-淀粉酶和α-葡萄糖苷酶，这些酶与糖尿病和肥胖症等代谢紊乱有关。

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引用次数: 0

The dietary supplementation of Sargassum fusiforme can effectively alleviate high-fat diet induced metabolic abnormalities 膳食补充马尾藻能有效缓解高脂饮食引起的代谢异常

IF 4.6 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2024-09-22 DOI: 10.1016/j.algal.2024.103722

Yu Bai , Yuanyuan Fu , Kang Chen , Yan Sun , Chengxu Zhou , Jichang Han , Xiaojun Yan

Sargassum fusiforme, a brown seaweed widely consumed as a culinary delicacy and medicinal herb in East Asian countries, contains multiple bioactive compounds with anti-inflammatory, antioxidant, and anti-obesity properties. This suggests that its dietary consumption could help mitigate metabolic diseases induced by high-fat diet (HFD). To test this hypothesis, we initially analyzed the nutritional components of the macroalga, and evaluated its acute oral toxicity and long-term safety. Subsequently, we explored the impacts of its dietary intake on HFD-fed mice. Additionally, 16S rRNA sequencing was employed to further elucidate the mechanism underlying the protective effects of this macroalga against HFD-induced metabolic abnormalities. Our results revealled that S. fusiforme has a desirable nutrient profile, characterized by rich carbohydrates, polyunsaturated fatty acids, and carotenoids, and is also highly safe for consumption. Its dietary intake remarkably alleviated HFD-induced metabolic disorders. Analysis of gut microbiota showed that HFD consumption increased the relative abundance of Firmicutes and decreased Bacteroidetes. This trend was further exacerbated by S. fusiforme supplementation, resulting in a higher Firmicutes/Bacteroidetes ratio compared to both the control and HFD groups. At the genus level, Muribaculum, Lactobacillus, Clostridia_UCG_014, Clostridium_sp, Acetatifactor, Eubacterium_coprostanoligenes, and IS_44 (from family Nitrosomonadaceae) were significantly enriched in the mice supplemented with S. fusiforme. These findings support the potential use of S. fusiforme as a functional food to counteract HFD-induced metabolic dysbiosis.

马尾藻（Sargassum fusiforme）是一种褐色海藻，在东亚国家作为美食和药材被广泛食用，它含有多种生物活性化合物，具有抗炎、抗氧化和抗肥胖的特性。这表明，从饮食中摄入这种物质有助于减轻高脂饮食（HFD）引起的代谢性疾病。为了验证这一假设，我们首先分析了大型藻类的营养成分，并评估了其急性口服毒性和长期安全性。随后，我们探讨了从膳食中摄取大叶藻对高脂饮食小鼠的影响。此外，我们还利用 16S rRNA 测序进一步阐明了这种大型藻类对 HFD 引起的代谢异常具有保护作用的机制。我们的研究结果表明，S. fusiforme具有理想的营养成分，富含碳水化合物、多不饱和脂肪酸和类胡萝卜素，食用安全性高。从膳食中摄入这种植物能显著缓解高氟酸诱导的代谢紊乱。对肠道微生物群的分析表明，摄入高频分解膳食会增加固着菌的相对丰度，而减少类杆菌的相对丰度。补充 S. fusiforme 进一步加剧了这一趋势，导致与对照组和高氟日粮组相比，固着菌/类杆菌的比例更高。在属的层面上，补充了扶桑花菌素的小鼠体内的Muribaculum、乳酸杆菌、梭状芽孢杆菌_UCG_014、梭状芽孢杆菌_sp、Acetatifactor、Eubacterium_coprostanoligenes和IS_44（来自亚硝基单胞菌科）显著富集。这些研究结果支持将扶桑花菌素作为一种功能性食品来对抗高氟酸膳食诱导的代谢紊乱。

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引用次数: 0

Topical formulations contained an extract from marine alga Cladophora glomerata 外用制剂含有海洋藻类 Cladophora glomerata 的提取物

IF 4.6 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2024-09-21 DOI: 10.1016/j.algal.2024.103717

Arpa Petchsomrit , Kunlathida Luangpraditkun , Naphatson Chanthathamrongsiri , Nadechanok Jiangseubchatveera , Nattawut Leelakanok , Thanchanok Sirirak

The Cladophora glomerata is a green alga abundant in fresh and seawater. The extraction of C. glomerata using glycol-based solvents, including butylene glycol (BG), polyethylene glycol 400 (PEG 400), and propylene glycol (PG), as the green solvents to obtain antioxidants and nutrients that can be further used in health care and pharmaceutical applications. The extraction of C. glomerata with glycol-base solvents using the microwave-assisted extraction (MAE) method was optimized. The liquid extraction using 60 % volume by volume of polyethylene glycol yielded the highest total phenolic content (4.00 ± 0.08 mg gallic acid equivalence per g dry weight) and antioxidant activity (IC₅₀ 0.14 ± 0.02 mg/mL). The algal extract was stable at pH 6 to 8 and a temperature lower than 25 °C. After incorporating the algal extract in topical formulations including serum and emulgel, we found that the preparations were stable and retained the extract's antioxidant activity. In addition, all algal extracts, extract-loaded serum, and emulgel did not show cytotoxicity effects in normal adult human dermal fibroblasts.

团扇藻是一种绿色藻类，在淡水和海水中含量丰富。使用乙二醇基溶剂（包括丁二醇（BG）、聚乙二醇 400（PEG 400）和丙二醇（PG））作为绿色溶剂萃取团扇藻，可获得抗氧化剂和营养物质，并可进一步应用于保健和制药领域。利用微波辅助萃取（MAE）法，对乙二醇基溶剂萃取草苁蓉进行了优化。使用体积分数为 60% 的聚乙二醇进行液体萃取时，总酚含量（每克干重 4.00 ± 0.08 毫克没食子酸当量）和抗氧化活性（IC50 0.14 ± 0.02 毫克/毫升）最高。海藻提取物在 pH 值为 6 至 8 和温度低于 25 °C 的条件下稳定。将海藻提取物加入血清和凝胶等外用制剂后，我们发现这些制剂非常稳定，并保留了提取物的抗氧化活性。此外，所有海藻萃取物、萃取物负载的血清和凝胶在正常成人真皮成纤维细胞中均未显示出细胞毒性效应。

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引用次数: 0

Use of plant growth regulators and their combined application to enhance lipid productivity in the filamentous microalga Tribonema sp. 使用植物生长调节剂及其联合应用提高丝状微藻 Tribonema sp.

IF 4.6 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2024-09-20 DOI: 10.1016/j.algal.2024.103721

Feifei Wang, Siyan Liu, Yuanhong Li, Na Zhang, Shuyi Li, Zhenzhou Zhu

Oleaginous Tribonema sp. has shown great potential in biodiesel production. However, effective strategies are still needed to increase overall lipid productivity for cost-effectiveness. Although the effects of plant growth regulators (PGRs) on enhancing microalgal growth and lipid content have been widely demonstrated, the synergistic effects of their combinations on improving lipid productivity in Tribonema sp. remain unclear. In this study, we investigated the comparative effects of five PGRs (e.g., fulvic acid, melatonin, gibberellin, γ-aminobutyric acid [GABA], and indoleacetic-3-acid [IAA]) at different concentrations (0.1–10 mg/L) on the growth, nitrogen uptake, and lipid content of Tribonema sp. The results showed that 10 mg/L GABA and 0.1 mg/L IAA were optimal conditions, contributing to an approximately 33.00 % increase in lipid productivity compared with the control (without PGRs). Based on these findings, the combination of optimized concentrations of GABA and IAA, especially in equal proportions, resulted in a more desirable fatty acid profile and produced the highest biomass, lipid content, and lipid productivity, with increases of 23.55 %, 25.62 %, and 54.95 %, respectively, over the control. This combination also significantly increased nitrogen removal efficiency in the medium, which was 46.24 % higher that of the control. These results were attributed to the significant upregulation of genes associated with nitrogen metabolism mediated by the synergy of IAA and GABA, promoting nitrate uptake by algal cells in the medium. Genes involved in photosynthesis and lipid synthesis were also upregulated, facilitating simultaneous biomass and lipid accumulation to maximize lipid productivity. Overall, this study is the first to report the combined application of GABA and IAA as an effective strategy to enhance lipid productivity in Tribonema sp., providing insights into the underlying mechanisms. These findings offer a promising solution for economically improving microalgal lipid production.

油脂性铁蒺藜在生物柴油生产中显示出巨大潜力。然而，为了提高成本效益，仍然需要有效的策略来提高总体脂质生产率。尽管植物生长调节剂（PGRs）对提高微藻生长和脂质含量的作用已被广泛证实，但它们的组合对提高铁蒺藜的脂质生产率的协同作用仍不清楚。在本研究中，我们研究了五种 PGRs（如结果表明，10 毫克/升 GABA 和 0.1 毫克/升 IAA 是最佳条件，与对照（无 PGRs）相比，脂质生产率提高了约 33.00%。基于这些发现，GABA 和 IAA 的最佳浓度组合（尤其是等比例组合）产生了更理想的脂肪酸谱，并产生了最高的生物量、脂质含量和脂质生产率，分别比对照组提高了 23.55 %、25.62 % 和 54.95 %。该组合还大大提高了培养基的脱氮效率，比对照组高出 46.24%。这些结果归因于在 IAA 和 GABA 的协同作用下，与氮代谢相关的基因显著上调，促进了培养基中藻类细胞对硝酸盐的吸收。参与光合作用和脂质合成的基因也被上调，从而促进生物量和脂质的同时积累，最大限度地提高脂质生产率。总之，本研究首次报道了联合应用 GABA 和 IAA 作为提高 Tribonema sp.这些发现为提高微藻脂质生产的经济效益提供了一种前景广阔的解决方案。

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引用次数: 0

Exploring quorum sensing for inducing bioactives overproduction in the dinoflagellate microalga Amphidinium carterae 探索通过定量感应诱导甲藻中生物活性物质的过量生产

IF 4.6 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2024-09-19 DOI: 10.1016/j.algal.2024.103719

S. Kichouh-Aiadi, J.J. Gallardo-Rodríguez, L. López-Rosales, M.C. Cerón-García, F. García-Camacho, A. Sánchez-Mirón

The dinoflagellate Amphidinium carterae is a promising source of unique bioactive compounds with potential applications in diverse industries. Abiotic strategies for increasing bioactive cell quota have been preferred for cost-effectiveness, controllability, and existing knowledge. The supernatants of Heterosigma akashiwo and Pavlova sp. enhanced haemolytic activity of A. carterae by 2.3 times and improved its final biomass by 10 % when added to the growth medium. Prymnesium parvum supernatant increased the total lipid content of A. carterae by 50 %, while Pleurochrysis roscoffensis supernatant boosted its total carotenoid content by 40 %. This study demonstrated a rapid screening strategy for evaluating growth responses and highlights the potential of using allelopathic strategies to enhance bioactive metabolite production. Utilizing supernatants for the formulation of growth media fosters a circular economy by repurposing resources, minimizing waste, and promoting sustainability in the microalgal biotechnological industry.

甲壳双鞭毛藻（Amphidinium carterae）是一种前景广阔的独特生物活性化合物来源，具有在不同行业应用的潜力。由于成本效益、可控性和现有知识，增加生物活性细胞配额的非生物策略已成为首选。Heterosigma akashiwo 和 Pavlova sp.的上清液加入生长培养基后，可将 A. carterae 的溶血活性提高 2.3 倍，并将其最终生物量提高 10%。Prymnesium parvum 上清液可将 A. carterae 的总脂质含量提高 50%，而 Pleurochrysis roscoffensis 上清液可将其总类胡萝卜素含量提高 40%。这项研究展示了一种评估生长反应的快速筛选策略，并强调了利用等位策略提高生物活性代谢物产量的潜力。利用上清液配制生长介质，可以重新利用资源，最大限度地减少浪费，促进微藻生物技术产业的可持续发展，从而推动循环经济的发展。

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引用次数: 0

Application of computational fluid dynamics in optimizing microalgal photobioreactors 应用计算流体动力学优化微藻光生物反应器

IF 4.6 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2024-09-18 DOI: 10.1016/j.algal.2024.103718

Behnam Amanna , Parisa A. Bahri , Navid R. Moheimani

Developing more efficient microalgal photobioreactors (closed cultivation systems) is impelled by increasing interest in microalgal cultivation to produce high-value products. Compared to open cultivation systems closed photobioreactors (PBRs) offer several advantages, including the potential for high productivity in a small footprint, ability to maintain a controlled environment, and their scalability for commercial production. Computational Fluid Dynamics (CFD) provides a powerful tool to simulate flow conditions of microalgal cultivation systems and analyze operational parameters which may affect cultivation key factors such as light (wavelength, intensity, and period of exposure), accessibility to nutrients, and mixing. Besides, geometric modifications employing CFD can accelerate the design process and considerably reduce the need for a thorough, empirical exploration of reactor configurations and the associated costs. This paper provides a comprehensive review of recent updates on the application of CFD in various microalgal cultivation systems with special focus on different geometry optimization of flat plate photobioreactors.

This review examines recent studies that have employed various multiphase and turbulence methods within the CFD framework to simulate fluid flow and turbulence within reactors, aiming to improve flow characteristics and enhance microalgal productivity. Specifically, the application of CFD in optimizing flat plate photobioreactors is explored, with researchers investigating a range of baffles and sparger configurations to enhance microalgal productivity. Additionally, the advantages of adopting CFD are discussed, and the potential future applications of CFD in microalgal cultivation are outlined.

人们对培养微藻以生产高价值产品的兴趣与日俱增，这推动了开发更高效的微藻光生物反应器（封闭式培养系统）。与开放式培养系统相比，封闭式光生物反应器（PBR）具有多项优势，包括占地面积小、生产效率高、能够保持受控环境以及可扩展的商业化生产。计算流体动力学（CFD）为模拟微藻培养系统的流动条件和分析可能影响培养关键因素（如光照（波长、强度和照射时间）、营养物质的可及性和混合）的操作参数提供了一个强大的工具。此外，利用 CFD 进行几何修改可加快设计过程，并大大减少对反应器配置和相关成本进行全面、经验性探索的需要。本文全面综述了 CFD 在各种微藻培养系统中应用的最新进展，并特别关注平板光生物反应器的不同几何形状优化。本综述考察了在 CFD 框架内采用各种多相和湍流方法模拟反应器内流体流动和湍流的最新研究，旨在改善流动特性，提高微藻产量。具体而言，研究人员探讨了 CFD 在优化平板光生物反应器中的应用，研究了一系列挡板和喷射器配置，以提高微藻产量。此外，还讨论了采用 CFD 的优势，并概述了未来 CFD 在微藻培养中的潜在应用。

{"title":"Application of computational fluid dynamics in optimizing microalgal photobioreactors","authors":"Behnam Amanna , Parisa A. Bahri , Navid R. Moheimani","doi":"10.1016/j.algal.2024.103718","DOIUrl":"10.1016/j.algal.2024.103718","url":null,"abstract":"<div><div>Developing more efficient microalgal photobioreactors (closed cultivation systems) is impelled by increasing interest in microalgal cultivation to produce high-value products. Compared to open cultivation systems closed photobioreactors (PBRs) offer several advantages, including the potential for high productivity in a small footprint, ability to maintain a controlled environment, and their scalability for commercial production. Computational Fluid Dynamics (CFD) provides a powerful tool to simulate flow conditions of microalgal cultivation systems and analyze operational parameters which may affect cultivation key factors such as light (wavelength, intensity, and period of exposure), accessibility to nutrients, and mixing. Besides, geometric modifications employing CFD can accelerate the design process and considerably reduce the need for a thorough, empirical exploration of reactor configurations and the associated costs. This paper provides a comprehensive review of recent updates on the application of CFD in various microalgal cultivation systems with special focus on different geometry optimization of flat plate photobioreactors.</div><div>This review examines recent studies that have employed various multiphase and turbulence methods within the CFD framework to simulate fluid flow and turbulence within reactors, aiming to improve flow characteristics and enhance microalgal productivity. Specifically, the application of CFD in optimizing flat plate photobioreactors is explored, with researchers investigating a range of baffles and sparger configurations to enhance microalgal productivity. Additionally, the advantages of adopting CFD are discussed, and the potential future applications of CFD in microalgal cultivation are outlined.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"83 ","pages":"Article 103718"},"PeriodicalIF":4.6,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2211926424003308/pdfft?md5=236ce503d7f777dd84f752d0a46c056d&pid=1-s2.0-S2211926424003308-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142314957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of strontium removal by Tetraselmis chui grown in bubble column photobioreactors 优化在气泡柱光生物反应器中生长的 Tetraselmis chui 对锶的去除效果

IF 4.6 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts

Pub Date : 2024-09-16 DOI: 10.1016/j.algal.2024.103716

Inés Segovia-Campos , Montserrat Filella , Muhammad Saad Bin Zahid , Luísa Barreira , Karl Perron , Daniel Ariztegui

Green microalgae of the class Chlorodendrophyceae have recently attracted the interest of researchers due to their ability to form micropearls (intracellular inclusions of amorphous calcium carbonate) highly enriched in Sr. The marine species Tetraselmis chui (included in the class Chlorodendrophyceae) shows high uptake of both stable and radioactive Sr isotopes and has been suggested as a potential candidate for the development of new bioremediation tools regarding radioactive ⁹⁰Sr pollution. In this study, we optimized Sr removal from seawater by growing T. chui in 1-L bubble column photobioreactors (PBRs) with and without CO₂ supply. Culturing T. chui in bubble column PBRs greatly improves cell production and Sr removal compared to previous studies. Furthermore, the addition of 10 mL L⁻¹ h⁻¹ CO₂ further accelerates T. chui growth and results in better Sr removal rates. This study presents promising results for the development of new bioremediation methods to treat ⁹⁰Sr pollution.

海洋物种 Tetraselmis chui（包括在 Chlorodendrophyceae 类中）显示出对稳定和放射性 Sr 同位素的高吸收率，并被认为是针对放射性 90Sr 污染开发新型生物修复工具的潜在候选者。在本研究中，我们通过在有二氧化碳供应和无二氧化碳供应的 1-L 气泡柱光生物反应器（PBR）中培养 T. chui，优化了从海水中去除 Sr 的过程。与之前的研究相比，在气泡柱光生物反应器中培养 T. chui 大大提高了细胞产量和硒去除率。此外，添加 10 mL L-1 h-1 CO2 可进一步加速 T. chui 的生长，并提高硒的去除率。这项研究为开发新的生物修复方法处理 90Sr 污染提供了可喜的成果。

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引用次数: 0