Animal Feed Science and Technology最新文献

{"title":"Assessment of different dietary soybean meals and corn fermented protein in practical diets for fingerling channel catfish.","authors":"Abel R. Paladines-Parrales,&nbsp;Khanh Q. Nguyen,&nbsp;Magida Tabbara,&nbsp;Uthpala Padeniya,&nbsp;Abigeal Adeyemi,&nbsp;Yoonhang Lee,&nbsp;Timothy J. Bruce,&nbsp;D. Allen Davis","doi":"10.1016/j.anifeedsci.2025.116239","DOIUrl":"10.1016/j.anifeedsci.2025.116239","url":null,"abstract":"<div><div>One of the most common difficulties for the aquaculture industry is the farmers' demand for low-cost feeds, which may result in reduced performance of the culture species. This study aimed to evaluate the potential of replacing solvent-extracted soybean meal, one of the primary low-cost protein sources in a practical catfish feeds with improved plant-based proteins. Four open feed formulations were tested which included: basal diet (32 % protein, 6 % lipid) contained 56.4 % soybean meal (SBM); basal modified to include 10 % of a corn-fermented protein (CFP) from the ethanol industry (Basal-CFP); the complete replacement of SBM with either enzyme-treated soybean meal (43 % inclusion, Basal-ET) or low oligosaccharide soybean meal (45 % inclusion, Basal-LO). Diets were evaluated under controlled indoor aquaria conditions (12-weeks) and under production condition over a 70-day culture period using in-pond raceways. Channel catfish reared in aquaria were offered the four test diets as well as a commercial catfish feed. In this trial, catfish demonstrated significantly better growth and fcr when fed the Basal-LO and Basal CFP diets indicating that commercial feed formulations can be improved. In both trials, channel catfish offered the Basal-LO and Basal-CFP diets generally did better than those reared on the Basal diet. Blood parameters were measured in both trials with limited differences in hematology. Gene expression analysis of the liver of fish reared under controlled conditions showed decreased hepatic hexokinase expression in the Basal-LO group and increased intestinal cholecystokinin expression in all diet groups compared to the control. This indicates metabolic alteration of appetite in fish in response to changes in the feed ingredients. These results suggest that newer soybean variants may be beneficial in improving the growth performance of channel catfish. Thus warranting continued work on improved ingredients in practical feed formulations for channel catfish.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"321 ","pages":"Article 116239"},"PeriodicalIF":2.5,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143218586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Essential oil of Curcuma aromatica as a dietary supplement: Evaluation of its effect on growth, haematology, immune response and histopathology in Channa punctata challenged with two pathogenic aeromonads","authors":"Rajdeep Das,&nbsp;Naisringdi Thaosen,&nbsp;Kangkan Sarma,&nbsp;Dandadhar Sarma","doi":"10.1016/j.anifeedsci.2025.116235","DOIUrl":"10.1016/j.anifeedsci.2025.116235","url":null,"abstract":"<div><div>A seven-week feeding trial was conducted to examine the effect of dietary inclusion of <em>Curcuma aromatica</em> essential oil (CAEO) as supplementary feed additive on growth performance, haematological parameters, immunological responses and histological changes in <em>Channa punctata</em>. A total of 675 fish (initial body weight of 4.44 ± 0.23 g) were randomly distributed in 27 tanks with 25 fish per tank (3 treatments with 3 replications) and fed with diets containing essential oil extracted from rhizome of <em>Curcuma aromatica</em> (CAEO) at concentrations 0.00, 0.25 and 2.00 mL per kg feed. Each of the experimental feeding groups was further divided into 3 sub-groups C: not challenged with bacteria, RD-01: challenged with <em>Aeromonas veronii</em>, RD-05: challenged with <em>Aeromonas hydrophila</em>. The first experimental phase consisted of feeding fish for the first four weeks, and in the second phase, the fish were challenged with LD₅₀ of <em>A. veronii</em> (2.6 × 10⁴ CFU/fish) and <em>A. hydrophila</em> (1.6 × 10⁴ CFU/fish) with continued feeding for remaining three weeks. The final weight gain and specific growth rate were found to be significantly increased (<em>P</em> &lt; 0.05) in the group fed with supplemented diet containing 2.00 mL/kg concentration. Cumulative mortality was recorded for 3 weeks post-challenge which showed higher percentage of survival rate (&gt;95 %) in groups fed with 2.00 mL CAEO/kg diet, where RD-01 and RD-05 sub-groups had a survival rate of 98 % and 96 %, respectively. The haematological values were significantly higher in both challenged and unchallenged groups fed with 2.00 mL CAEO/kg diet. The total protein content, total immunoglobulin concentration and lysozyme activity significantly increased in groups fed with 2.00 mL CAEO/kg diet, in which RD-05 showed the highest values. Comparable histological differences were prominent in liver and kidney tissues of fish fed with 0.00 and 2.00 mL/kg diets. The study illustrated that the dietary supplementation with 2.00 mL CAEO/kg feed is highly effective to enhance growth and health in <em>Channa punctata</em> and increase bacterial resistance to <em>Aeromonas</em> species.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"321 ","pages":"Article 116235"},"PeriodicalIF":2.5,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143218590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predicting digesta retention times from dietary physicochemical properties in pigs","authors":"Sebastian Dorado-Montenegro ,&nbsp;Joshua A. Dijksman ,&nbsp;Walter J.J. Gerrits ,&nbsp;Sonja de Vries","doi":"10.1016/j.anifeedsci.2025.116242","DOIUrl":"10.1016/j.anifeedsci.2025.116242","url":null,"abstract":"<div><div>Dietary physicochemical properties (PP) partially modulate digesta passage along the gastrointestinal tract (GIT). Understanding which dietary PP influence digesta passage is relevant for predicting nutrient absorption kinetics. This study assessed the potential of dietary PP for predicting mean retention time (MRT) and phase segregation of digesta, combining data from four experiments (21 diets, n = 166 pigs, and body weight = 42 ± 14.0 kg) including diets broadly ranging in PP. The MRT of digesta in various GIT segments, measured using similar methodology, was related to dietary PP: rheological parameters (extract viscosity and solid phase moduli plus linear viscoelastic range) and water-binding capacity (WBC). For each GIT segment and digesta phase, PP were selected as independent variables to include in prediction models after a correlation and multicollinearity analysis, followed by MaxR and stepwise variable selection methods. Subsequently, multiple and single regression analyses were performed, including selected PP as predictor variables. The MRT of solid (MRT_solids) and liquid (MRT_liquids) digesta, and digesta phase segregation were predicted using model equations with (1) selected PP (full model), (2) WBC only, and (3) extract viscosity only; and calculated vs. observed values were compared. Coefficients of determination (R²) were used to select the best model to predict digesta MRT by GIT segment. Dietary PP were capable of predicting digesta phase segregation in the stomach (R² = 0.56), largely explained by WBC (R² = 0.51), and MRT_liquids (R² = 0.26), explained by WBC and rheological parameters. Dietary PP were less effective at predicting MRT in the small and large intestines (R² = 0.07–0.17), though phase segregation in the cecum was better predicted (R² = 0.26). Full PP-models, predicted MRT_solids and MRT_liquids in all segments more accurately than dietary WBC or extract viscosity alone, whereas WBC was the most important predictor of phase segregation in the stomach. Extract viscosity explained negligible variation in digesta passage. This information can be used in feed formulation to better anticipate how dietary PP influence digestion kinetics.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"321 ","pages":"Article 116242"},"PeriodicalIF":2.5,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143218559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of propionic acid as a feed additive and grain preservative on weanling pig performance and digestive health","authors":"Kathryn Ruth Connolly ,&nbsp;Torres Sweeney ,&nbsp;Dillon Padraig Kiernan ,&nbsp;Alex Round ,&nbsp;Marion T. Ryan ,&nbsp;Vivian Gath ,&nbsp;Shane Maher ,&nbsp;Stafford Vigors ,&nbsp;John V. O’Doherty","doi":"10.1016/j.anifeedsci.2025.116237","DOIUrl":"10.1016/j.anifeedsci.2025.116237","url":null,"abstract":"<div><div>This study aimed to determine the effects of propionic acid (PA) as a grain preservative and feed supplement on piglet growth and health post-weaning (PW). Grain was dried or preserved with 4 kg/tonne of PA post-harvest, and PA was supplemented or not at 4 kg/tonne during diet manufacture. Ninety-six weaned piglets were assigned to a 2 × 2 factorial design: (T1) dried grain diet, (T2) preserved grain diet, (T3) dried grain diet with PA and (T4) preserved grain diet with PA for 35 days. Each diet contained 450 g/kg of wheat and 150 g/kg of barley, either dried or preserved. Ten days PW, 28 piglets (n = 7) were euthanised for sample collection. Preserved grain reduced ochratoxin A and deoxynivalenol levels and increased body weight, average daily gain and average daily feed intake compared to dried grain (P &lt; 0.05). Supplementing PA increased the relative abundance of <em>Faecalibacterium</em> in the dried grain diet, but reduced <em>Faecalibacterium</em> in the preserved grain diet. Supplementing PA reduced ileal <em>Escherichia</em> in dried grain, but increased <em>Escherichia</em> in preserved grain (P &lt; 0.05). Supplementing PA increased colonic <em>Clostridium</em> in preserved grain but had no effect on <em>Clostridium</em> in dried grain (P &lt; 0.05). Supplementing PA reduced inflammatory cytokine expression in the duodenum (<em>IL1B/CXCL8/IL17/IL22/TLR4),</em> jejunum <em>(IL17</em>/<em>IL22)</em> and ileum (<em>IL22)</em> compared to non-PA supplemented diets (P &lt; 0.05). In conclusion, preserved grain improved grain quality, microbial populations and growth. Supplementing PA reduced intestinal inflammatory markers, suggesting potential as a feed additive in stress-inducing commercial settings.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"321 ","pages":"Article 116237"},"PeriodicalIF":2.5,"publicationDate":"2025-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143218564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of sodium benzoate on the quality and aerobic stability of corn silage contaminated with Listeria monocytogenes","authors":"E. Tüzel ,&nbsp;E. Tegün ,&nbsp;R. Aydin","doi":"10.1016/j.anifeedsci.2025.116238","DOIUrl":"10.1016/j.anifeedsci.2025.116238","url":null,"abstract":"<div><div>This study was conducted to evaluate the effects of sodium benzoate on the microbial safety and fermentation quality of corn silage contaminated with <em>Listeria monocytogenes</em>. Corn plants were harvested during the milking stage, chopped into 1.5–2.0 cm segments, and ensiled in 1.5-liter containers at ambient temperature for 60 days. The experimental design included four treatment groups, each with four replicates: a control group (Group A), a group inoculated with <em>L. monocytogenes</em> (Group B), a group treated with both <em>L. monocytogenes</em> and sodium benzoate (Group C), and a group treated with sodium benzoate alone (Group D). <em>L. monocytogenes</em> was introduced to Groups B and C at a concentration of 10⁵ cfu/g, while sodium benzoate was added to Groups C and D at a concentration of 0.1 %. Following the 60-day ensiling period, samples were subjected to comprehensive chemical and microbiological analyses, as well as a 5-day aerobic stability test. Sodium benzoate supplementation enhanced the concentration of water-soluble carbohydrates and reduced levels of butyric acid, ethanol, and ammonia nitrogen (P &lt; 0.001). Post-aerobic stability test results indicated that silages in Group D exhibited lower pH, CO₂ production, and yeast and mold counts significantly compared to the control group. No <em>L. monocytogenes</em> colony growth was observed in the silages during the microbiological analysis. These findings suggested that sodium benzoate could be a potent additive for improving both the fermentation quality and microbial safety of silages, offering significant benefits in the context of animal nutrition.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"321 ","pages":"Article 116238"},"PeriodicalIF":2.5,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143218427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-Inflammatory potential of lactic acid bacteria for dairy cows during the periparturient period","authors":"Yi Ma ,&nbsp;Xingliang Gao ,&nbsp;Mabrouk Elsabagh ,&nbsp;Yong Feng ,&nbsp;Yong Chen ,&nbsp;Long Cheng ,&nbsp;Zelin Li ,&nbsp;Huayou Chen ,&nbsp;Fuyuan Liu","doi":"10.1016/j.anifeedsci.2025.116234","DOIUrl":"10.1016/j.anifeedsci.2025.116234","url":null,"abstract":"<div><div>The prevalence of inflammatory diseases in peripartum dairy cows is associated with changes in bovine immune responses. Although antibiotics can treat related inflammatory diseases, their prominent issues such as antibiotic resistance are significant reasons limiting their use. The supplementation of lactic acid bacteria (LAB) has been shown to offer anti-inflammatory benefits, yet its efficacy varies with species and strain specificity. This review delineates the common types of inflammation and their triggers in peripartum dairy cows and scrutinizes case studies involving LAB application, specifying the LAB strains, dosages, and administration methods. It then elucidates various anti-inflammatory pathways and potential mechanisms of LAB, acknowledging that many insights are derived from non-bovine models, which poses a limitation in directly extrapolating findings to dairy cattle. The review suggests future research directions, such as strain screening, genetic engineering, dosage optimization, delivery methods, and mechanistic elucidation, to enhance the understanding of LAB's role in inducing pathogen resistance, enhancing intestinal barriers, and modulating immunity in peripartum dairy cows. This comprehensive overview aims to improve health outcomes by providing a more nuanced view of how LAB can support dairy cow health, with a caveat regarding the reliance on non-bovine studies.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"321 ","pages":"Article 116234"},"PeriodicalIF":2.5,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143218426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary bamboo vinegar powder improves growth performance, immunity, antioxidant capacity and lipid metabolism of largemouth bass (Micropterus salmoides)","authors":"Weibin Lai ,&nbsp;Shuwen Xu ,&nbsp;Ran Yu ,&nbsp;Linyi Li ,&nbsp;Yuanyi Zuo ,&nbsp;Manqi Yang ,&nbsp;Liangliang Zhang ,&nbsp;Liming Lu ,&nbsp;Yong Xu ,&nbsp;Yiwen Liu ,&nbsp;Hua Wang ,&nbsp;Jianchun Jiang","doi":"10.1016/j.anifeedsci.2025.116236","DOIUrl":"10.1016/j.anifeedsci.2025.116236","url":null,"abstract":"<div><div>Bamboo vinegar powder (BVP), a secondary product of bamboo charcoal production, contains multiple bioactive compounds. However, its effects on largemouth bass remain unclear. To investigate these effects, six experimental diets containing different BVP levels (0, 0.5, 1.0, 2.0, 4.0, and 8.0 g/kg) were designed, and a 56-day feeding experiment was conducted on largemouth bass (<em>Micropterus salmoides</em>, 7.00 ± 0.02 g). Results demonstrated significant increase in the weight gain rate (<em>P</em> &lt; 0.05) and specific growth rate (<em>P</em> &lt; 0.05) and decrease in the feed conversion ratio (<em>P</em> &lt; 0.05) with administration of diet containing 2.0 g/kg BVP. In addition, BVP (0.5–8.0 g/kg) significantly improved the levels of health indicators, such as aspartate aminotransferase, alanine transaminase, and alkaline phosphatase (<em>P</em> &lt; 0.05) in the liver. In terms of antioxidative properties, BVP (1.0–2.0 g/kg) improved the levels of antioxidant enzymes, including total antioxidant capacity (<em>P</em> &lt; 0.05) and catalase (<em>P</em> &lt; 0.05), reduced the malondialdehyde content (<em>P</em> &lt; 0.05), and upregulated the expression of nuclear factor erythroid 2-related factor 2 (<em>P</em> &lt; 0.05). Regarding lipid metabolism, BVP (1.0–8.0 g/kg) decreased the triglyceride (<em>P</em> &lt; 0.05) and total cholesterol (<em>P</em> &lt; 0.05) levels in the liver and improved hepatic lipid metabolism by upregulating the expression of lipid transport (hormone-sensitive lipase and lipoprotein lipase) and lipid metabolism (fatty acid transport protein and microsomal triglyceride transfer protein) genes (<em>P</em> &lt; 0.05). With regard to inflammation, BVP (0.5–4 g/kg) modulated the expression of inflammatory factors (interleukin-8, transforming growth factor-β, and interleukin-10; <em>P</em> &lt; 0.05) to improve the inflammatory response. Collectively, these results suggested that dietary BVP at a level of 2.0 g/kg can help improve growth performance, antioxidant capacity, and inflammatory response and reduce lipid deposition in the liver of largemouth bass.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"321 ","pages":"Article 116236"},"PeriodicalIF":2.5,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143218587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Natural feed additives in high-concentrate diets fed to Angus × Nellore finishing steers","authors":"Nelson Guimarães Dantas Canuto ,&nbsp;Henrique Jorge Fernandes ,&nbsp;Luís Carlos Vinhas Ítavo ,&nbsp;Marcelo Vedovatto ,&nbsp;Dalton Mendes de Oliveira ,&nbsp;Thomas Henrique Roque da Silva ,&nbsp;Amarildo Pedro da Silva ,&nbsp;Aline Gomes da Silva","doi":"10.1016/j.anifeedsci.2025.116233","DOIUrl":"10.1016/j.anifeedsci.2025.116233","url":null,"abstract":"<div><div>The study objective was to evaluate the use of naturally sourced feed additives to replace monensin in a high-concentrate diet on performance, behavior, and carcass characteristics. Eighty F1 Angus × Nellore steers [392 ± 2.6 kg of initial body weight (BW)] were assigned to 1 of 4 treatments: (1) monensin (29.5 mg/kg DM), (2) active yeast (85.0 mg/kg DM), (3) monensin and active yeast combined (at the same doses as above), and (4) a blend of essential oils (33.6 mg/kg DM). The basal diet (BD) had a roughage: concentrate ratio of 6:94. Sugarcane silage was used as roughage. The steers spent approximately 520 min resting (72 % of the daytime period), approximately 130 min eating (18 % of the daytime period), and 61 min ruminating (8.5 % of the daytime period). No difference was observed for dry matter (DM) intake during the adaptation phase (P &gt; 0.05). Steers receiving yeast had the lowest average daily gain (ADG) in the adaptation phase (P &lt; 0.05), however, this lower ADG did not have an impact on the final BW (P &gt; 0.05). On the other hand, steers receiving essential oils presented a ADG 26.5 % lower than the others during the experimental phase (P &lt; 0.05), which led to 18.3 kg lower (P &lt; 0.05) final BW of these steers (P &lt; 0.05). Differences in rib eye area and subcutaneous fat thickness can be attributed to the differences in the final BW and it is possible to infer that all the steers were at the same point of body development. We conclude that monensin can be replaced by active dry yeast in high-concentrate diets without compromising growth. Still, due a \"lag time\" for adequate rumen colonization of the yeast, steers-fed essential oils performed better during the adaptation phase. Then, essential oils (or other natural antimicrobial additives) combined with yeast are an option for adapting, which should be evaluated in future experiments.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"321 ","pages":"Article 116233"},"PeriodicalIF":2.5,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143218423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of a monounsaturated fatty acid-enriched fat infusion on fatty acid composition in various lipid fractions of plasma and follicular fluid of 46-day versus 67-day postpartum dairy cows","authors":"Y.J. Liu ,&nbsp;M. Plante-Dubé ,&nbsp;R. Lessard ,&nbsp;P.Y. Chouinard ,&nbsp;K. Tilleman ,&nbsp;B. Vlaeminck ,&nbsp;P. De-Sutter ,&nbsp;R. Gervais ,&nbsp;V. Fievez","doi":"10.1016/j.anifeedsci.2025.116232","DOIUrl":"10.1016/j.anifeedsci.2025.116232","url":null,"abstract":"<div><div>The composition of fatty acids (FA) in follicular fluid (FF) can be influenced by both dietary FA and endogenous lipid mobilization. Since the latter predominantly occurs during early lactation, we compared the impact of dietary lipid supplementation on the FA composition in FF lipid classes at two distinct time points in lactation. Furthermore, we investigated how the correlations between plasma and FF FA were affected by lipid supplementation and postcalving time. Twenty Holstein multiparous cows were randomly assigned to 200 g/d of prilled saturated fat in the rumen (n = 10) or sea buckthorn oil (SBT) in the abomasum (n = 10); specially, prilled saturated fat supplements enriched in palmitic acid (82 % 16:0; PA treatment) while SBT treatment included 27 % 16:1n-7, 28 % 16:0, 7.8 % 18:1n-7, 22 % 18:1n-9 and 11 % 18:2n-6. The treatment period ranged from 20 ± 5 d precalving to 67 ± 2 d (mean ± standard deviation) postcalving. Plasma and FF samples were collected from 2 sequential sessions of ovum pick-up (OPU-1 and OPU-2) at 46 and 67 ± 2 d postcalving. Except for 18:1n-9, proportions of the main unsaturated FA, supplied by SBT (i.e. 16:1n-7, 18:1n-7, and 18:2n-6) were increased (<em>P</em>_<em>fat</em> &lt; 0.05) in most lipid fractions of plasma and FF as compared with PA group. Nevertheless, - particularly for 16:1n-7 - the response depended on the sampling time (<em>P</em>_{<em>fat x time</em>} &lt; 0.10) was of lower magnitude at OPU-1. Despite the 18:1n-9 supply to the SBT group, proportions of this FA in the phospholipid (PL) and cholesterol esters (CHE) fractions of plasma and FF were lower as compared with the PA group (<em>P</em>_<em>fat</em> &lt; 0.001). However, in the plasma PL fraction the difference between fat sources was greater at OPU-1 than at OPU-2 (<em>P</em>_{<em>fat x time</em>} &lt; 0.05). Additionally, the ratio of 18:1n-9–18:0 decreased in the plasma non-esterified FA (NEFA) and PL fractions from OPU-1 to OPU-2 (<em>P</em>_{<em>time</em>} &lt; 0. 10), while the ratio increased in FF NEFA and CHE (<em>P</em>_{<em>time</em>} &lt; 0.05), irrespective of the fat source (<em>P</em>_{<em>fat x time</em>} &gt; 0.05 except for plasma PL). Differences between lactation stages, either or not dependent on fat supplementation, were hypothesized to be related to the (need for) alleviation of lipotoxicity through endogenous desaturation. Furthermore, except for 16:1n-7, plasma and FF NEFA were uncorrelated, while esterified plasma and FF FA showed higher correlations at OPU-2 than OPU-1. As such, (dietary) interventions with MUFA-enriched supplements potentially are less effective earlier after calving.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"321 ","pages":"Article 116232"},"PeriodicalIF":2.5,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143218428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary characteristics and body weight input improve the prediction of enteric methane production from growing-finishing pigs","authors":"Elvira Sattarova ,&nbsp;Jinghui Li ,&nbsp;James G. Fadel ,&nbsp;Knud E. Bach Knudsen ,&nbsp;Michael J. Hansen ,&nbsp;Jan V. Nørgaard ,&nbsp;Henry J.H. Jørgensen","doi":"10.1016/j.anifeedsci.2025.116231","DOIUrl":"10.1016/j.anifeedsci.2025.116231","url":null,"abstract":"<div><div>The objective of the present study was to identify dietary and animal factors that affect enteric methane (CH₄) production in growing-finishing pigs. Moreover, the study aims to develop and compare the predictive accuracy of the developed models with the existing equations using the Tier 1 approach and equations considering nutrient availability. A dataset from 13 studies containing 290 individual animal observations on 107 growing-finishing pigs was used to develop the prediction model for enteric CH₄ production. The predictive ability of the newly developed models was evaluated and compared with the extant equation proposed by the Intergovernmental Panel on Climate Change (IPCC) Tier 1 approach and extant prediction equations that consider substrate available for fermentation. Production of enteric CH₄ was predicted by fitting mixed-effects models with random effects of animal and study. The newly developed models for enteric CH₄ production, which include dietary characteristics and animal body weight, surpass the current IPCC Tier 1 model used in national inventories, demonstrating better precision and accuracy. Therefore, the equations developed in this study can be used in the pork industry to estimate the effect of feeding strategies to mitigate enteric CH₄ production from growing-finishing pigs and to improve the national inventories of CH₄ production.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"321 ","pages":"Article 116231"},"PeriodicalIF":2.5,"publicationDate":"2025-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143218558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}