Animal Feed Science and Technology最新文献

{"title":"Evaluation of phytogenic product supplementation on the growth performance, immune function, and gut microbiota composition in fingerling channel catfish (Ictalurus punctatus)","authors":"Ishini A. Appuhami ,&nbsp;D. Allen Davis ,&nbsp;Yoonhang Lee ,&nbsp;Abdulmalik A. Oladipupo ,&nbsp;Brent M. Vuglar ,&nbsp;Fernando Y. Yamamoto ,&nbsp;Mark Farmer ,&nbsp;Timothy J. Bruce","doi":"10.1016/j.anifeedsci.2025.116575","DOIUrl":"10.1016/j.anifeedsci.2025.116575","url":null,"abstract":"<div><div>Phytogenic feed additives derived from plant-based sources like essential oils and extracts are increasingly utilized in aquaculture. They have the potential to bolster immune responses, enhance disease resistance, and improve growth performance in fish. The current study investigated the effect of commercial phytogenic feed additives (Actifor Pro, Actifor Power, Fresta Protect, Syrena Boost, and Enviro QS) on growth performance, immunity and disease susceptibility of juvenile channel catfish (<em>Ictalurus punctatus</em>) within an indoor recirculating system. Over eight weeks, 750 juvenile channel catfish (14.8 ± 0.5 g, initial weight) were stocked in 30 tanks (25 fish tank⁻¹) and fed either a control or five phytogenic-supplemented diets. Growth performance, including weight gain, survival, and feed conversion ratio, was similar among treatments (<em>P</em> &gt; 0.05). Following the feeding trial, fish were challenged with virulent <em>Aeromonas hydrophila</em> (vAh; ML09–119; 1.86 × 10⁷ CFU mL⁻¹ immersion dose in water). Seven days post-exposure to vAh, Kaplan-Meier survival analysis indicated improved survival probability in the phytogenic-treated groups (<em>P</em> = 0.009). Sera lysozyme activity significantly differed across the groups (<em>P</em> = 0.014); moreover, the catfish fed with Fresta Protect had higher sera lysozyme activity than the control group. Biochemical testing of an additional thirteen markers in the sera, including ions, glucose, digestive enzymes, and cholesterol, did not reveal any treatment-related differences. The expression of immune-related genes (<em>il1b, il8, tnfa</em>, and <em>tgfb</em>) was not affected after eight weeks of the feeding trial, regardless of the dietary treatment. 16S rRNA sequencing showed that <em>Cetobacterium somerae</em> was the most abundant gut microbe across all diets, with the highest prevalence in the Syrena Boost group (80.54 %). While the overall microbial diversity did not change significantly between treatments, dietary phytogenic compounds influenced the presence or abundance of certain bacterial species, such as <em>Cellulosilyticum lentocellum</em>, <em>Clostridium disporicum</em>, <em>Plesiomonas shigelloides</em>, and <em>Niameybacter massiliensis</em>.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"331 ","pages":"Article 116575"},"PeriodicalIF":2.7,"publicationDate":"2025-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145616037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary polyunsaturated fatty acids supplementation improves growth, immunity, and gut microbiota composition in the fish Amphiprion ocellaris","authors":"Sun Qi ,&nbsp;Sarfraz Hussain ,&nbsp;Wang Boyu ,&nbsp;Yang Xuewei ,&nbsp;Jing Jiaojiao ,&nbsp;Li Shuangfei ,&nbsp;Naveed Ahmed","doi":"10.1016/j.anifeedsci.2025.116573","DOIUrl":"10.1016/j.anifeedsci.2025.116573","url":null,"abstract":"<div><div>Polyunsaturated fatty acids (PUFAs) are essential to human and animal physiology, and are commonly found in dietary supplements. However, the effects of PUFAs on the development, immunity, and gut microbiota of <em>Amphiprion ocellaris</em> are unclear. This study investigated the effects of PUFAs from the microalga <em>Schizochytrium sp</em>. on the growth, immunity, and gut microbial community composition of <em>A. ocellaris</em>. PUFAs promoted fish development, with the 7 % PUFAs group showing higher weight gain (WGR) and specific growth rate (SGR) (107.63 % day⁻¹ and 0.73 % day⁻¹, respectively). A lower amount (5 %) of PUFAs had higher feed conversion ratios (FCRs) than the higher amount of PUFAs (7 %) and no addition of PUFAs. Metagenomic results showed notable fluctuations in the microbial composition, particularly a reduction in the viral community in the PUFA-treated groups. Immunological parameters showed that PUFA supplementation increased liver lysozyme, IgM, and superoxide dismutase (SOD) levels, suggesting a better response to oxidative stress. Microbial diversity was negatively associated with immunological response, particularly blood IgM levels. These results indicate that PUFA supplementation may improve fish development and immunity, but excessive PUFA consumption may harm gut microbial diversity and immunity. This work contributes to producing alternative food supplements that can effectively promote growth and improve fish immunity.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"331 ","pages":"Article 116573"},"PeriodicalIF":2.7,"publicationDate":"2025-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145546863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Methane emissions and ruminal fermentation in 22-month-old dairy-beef heifers, previously weaned at different ages, grazing ryegrass or chicory herbage in summer","authors":"Maria M. Della Rosa,&nbsp;Arjan Jonker,&nbsp;Alicia Barnett,&nbsp;Peter H. Janssen,&nbsp;Juan F. Uribe-Gomez,&nbsp;Paul Maclean,&nbsp;Ignacio Lizarraga,&nbsp;M. Ajmal Khan","doi":"10.1016/j.anifeedsci.2025.116572","DOIUrl":"10.1016/j.anifeedsci.2025.116572","url":null,"abstract":"<div><div>Early-life management can influence methane (CH₄) production in ruminants after weaning, and the type of forage fed to animals can interact with the early-life interventions. However, the persistence of the effect of early life interventions has been little studied in cattle. The study aim was to evaluate the interaction effect between weaning age (10 vs. 20 weeks) and diet on CH₄ production and ruminal fermentation from dairy-beef heifers when they were 22-months old. Hereford × Friesian female calves were weaned from milk at 10 weeks of age (n = 36) or at 20 weeks of age (n = 36). When the heifers were 22-months old and weighed 523 ± 33 kg (mean ± standard deviation), they grazed <em>ad libitum</em> mature ryegrass-based herbage (<em>Lolium perenne</em>; RG) or <em>ad libitum</em> chicory (<em>Cichorium intybus</em>; CHI) in a crossover design with two 21-day periods. Methane and carbon dioxide (CO₂) production were measured using GreenFeed units. There was no effect of weaning age or of weaning age × diet interaction on CH₄ (g/d) and CO₂ (kg/d) production, residual CH₄ and acetate plus butyrate to propionate plus valerate (AB:PV) ratio. Methane production was 6 % less (165 ± 25 vs 168 ± 26 g/d) and CO₂ production was 12 % greater (8.1 ± 0.6 vs 7.2 ± 0.5 kg/d) and AB:PV was 6 % greater in heifers grazing CHI compared to those grazing RG. Methane production was therefore lower from heifers grazing CHI than from heifers grazing RG, but the weaning age intervention resulted in a similar CH₄ production in 22-month-old cattle.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"331 ","pages":"Article 116572"},"PeriodicalIF":2.7,"publicationDate":"2025-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145546862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Short communication: Performance and gene expression in broilers from breeders of different ages, supplemented or not with 1,25(OH)2D3-glycoside","authors":"E.A. Iachinski ,&nbsp;T.S. Andrade ,&nbsp;N. Rohloff Junior ,&nbsp;B.A. Bebber ,&nbsp;R.A. Bombardelli ,&nbsp;J.G. Vargas Junior ,&nbsp;A.A. Calderano ,&nbsp;C. Eyng ,&nbsp;R.V. Nunes","doi":"10.1016/j.anifeedsci.2025.116571","DOIUrl":"10.1016/j.anifeedsci.2025.116571","url":null,"abstract":"<div><div>This study aimed to investigate the performance and gene expression of calbindin-D28K (CALB), interleukin-10 (IL-10), and interleukin-1β (IL-1β) in 21-day-old broilers from breeders of different ages, supplemented or not with 1,25-dihydroxycholecalciferol glycoside (1,25(OH)₂D₃-glycoside). A total of 8000 AP95 (Aviagen) breeders, at 21 weeks of age, were evenly distributed between two houses. In one house, the birds received a corn and soybean meal-based diet supplemented with 100 mg/kg of 1,25(OH)₂D₃-glycoside (providing 1 µg of active 1,25(OH)₂D₃ per kg of feed), while in the other, the same diet was provided without the supplementation. When the breeders reached 30, 46, and 62 weeks of age, their eggs were incubated. After hatching, three trials were conducted, each with 384 one-day-old male broilers originating from breeders aged 30 (Trial I) 46 (Trial II), and 62 weeks (Trial III), respectively. All trials consisted of two treatments, based on whether the breeders’ diets were supplemented with 1,25(OH)₂D₃-glycoside or not. In every trial, broilers were fed a diet free of 1,25(OH)₂D₃-glycoside until 21 days of age, meeting the nutritional requirements for this phase. Breeders aged 46 and 62 weeks, supplemented with 1,25(OH)₂D₃-glycoside, produced broilers with an initial average weight superior to that of broilers from breeders without supplementation. Comparing the trials, broilers from breeders aged 46 and 62 weeks showed higher feed intake, greater weight gain, and better feed conversion at 21 days of age. Broilers from breeders aged 62 weeks, supplemented with 1,25(OH)₂D₃-glycoside, showed higher gene expression of CALB, IL-10, and IL-1β at 21 days of age compared to broilers from breeders that did not receive 1,25(OH)₂D₃-glycoside. Comparing the trials, broilers from breeders aged 46 and 62 weeks supplemented with 1,25(OH)₂D₃-glycoside had the highest gene expression of IL-10 and IL-1β. It is concluded that breeders aged 46 and 62 weeks, when supplemented with 1,25(OH)₂D₃-glycoside, produce broilers with higher weight at hatch, as well as enhanced performance and increased gene expression of CALB, IL-10, and IL-1β at 21 days of age.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"331 ","pages":"Article 116571"},"PeriodicalIF":2.7,"publicationDate":"2025-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145578142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Improving lipid utilization and growth through lecithin inclusion in diets for giant grouper (Epinephelus lanceolatus)” [Anim. Feed Sci. Technol. 326 (2025) 116393]","authors":"Grace Butler ,&nbsp;Caroline Lourdes Candebat ,&nbsp;Simon Kumar Das ,&nbsp;Leo Nankervis","doi":"10.1016/j.anifeedsci.2025.116569","DOIUrl":"10.1016/j.anifeedsci.2025.116569","url":null,"abstract":"","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"330 ","pages":"Article 116569"},"PeriodicalIF":2.7,"publicationDate":"2025-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145517377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mixture extracts of Psidium guajava L. and Azadirachta indica A. Juss - based diets synergistically improve health status through red tilapia (Oreochromis sp.) immune responses, antioxidant activities, and resistance to bacterial pathogens","authors":"Truong Quynh Nhu ,&nbsp;Bui Thi Bich Hang ,&nbsp;Nguyen Thi Mai ,&nbsp;Nguyen Do Quynh ,&nbsp;Tran Thi Tuyet Hoa ,&nbsp;Nguyen Thi Thu Hang ,&nbsp;Nguyen Thi Kim Ha ,&nbsp;Tran Ngoc Bich ,&nbsp;Vu Ngoc Minh Thu ,&nbsp;Nguyen Thanh Phuong ,&nbsp;Patrick Kestemont","doi":"10.1016/j.anifeedsci.2025.116570","DOIUrl":"10.1016/j.anifeedsci.2025.116570","url":null,"abstract":"<div><div>The study investigated the effects of guava and neem extract-based diets, alone or in combination, on the immune responses, antioxidative activities and disease resistance in red tilapia (<em>Oreochromis</em> sp.). Fish were randomly assigned to seven treatments: a control diet (without extract), single guava extract (0.3 %), single neem extract (0.3 %), and guava-neem mixture at 0.1 %:0.05 %, 0.2 %:0.1 %, 0.4 %:0.2 %, and 0.8 %:0.4 % for 6 weeks, followed by being challenged with <em>Streptococcus agalactiae</em>. The results showed that single extract diets did not affect total red blood cells, hematocrit, or white blood cells. In contrast, mixture diets induced strong synergistic effects, significantly enhancing total white blood cells at weeks 3 and 6 compared to the control (p &lt; 0.05). At equivalent total extract concentration (0.3 %), mixture diets synergistically enhanced respiratory burst activity in blood cells in W6 and 3 days post-challenge compared to single and control groups (p &lt; 0.05). Similarly, mixture diets significantly enhanced the humoral immune responses (lysozyme, complement, alkaline phosphatase and peroxidase) in time and dose-dependent manners, while the single extract produced a weaker response compared to control. Antioxidant capacity was markedly improved by the mixture diets (except 0.8 %:0.4 %), with synergistic increases in superoxide dismutase and catalase, accompanied by reduced malondialdehyde levels compared to single and control groups (p &lt; 0.05). Importantly, only the mixture diets significantly reduced cumulative mortality after bacterial infection (p &lt; 0.05), whereas single extracts offered no protection compared to control (p &gt; 0.05). Histological assessment confirmed that the highest mixture (0.8 %:0.4 %) caused no damage to the liver or gut. Dietary supplementation with guava-neem mixtures, particularly at 0.1 %:0.05 %, 0.2 %:0.1 %, and 0.4 %:0.2 %, elicited synergistic enhancements in immune and antioxidant status, providing better protection for red tilapia against <em>S. agalactiae</em> infection.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"330 ","pages":"Article 116570"},"PeriodicalIF":2.7,"publicationDate":"2025-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145569044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Performance and nutrient digestibility of broilers fed barley-based diets supplemented with amylase","authors":"Lucas S. Bassi ,&nbsp;Khaled Itani ,&nbsp;Muhammad Ahmad ,&nbsp;Adam Smith ,&nbsp;Aaron Cowieson ,&nbsp;Sebastian Kaczmarek ,&nbsp;Birger Svihus","doi":"10.1016/j.anifeedsci.2025.116568","DOIUrl":"10.1016/j.anifeedsci.2025.116568","url":null,"abstract":"<div><div>This study evaluated the effects of exogenous amylase supplementation in barley-based diets for broiler chickens. Two experiments (Exp. 1 and 2) were conducted, in which 11-to-33-<span>d</span>-old broilers were assigned to two dietary treatments (with or without amylase at 81 kg-novo units/kg diet) in a randomized design. Exp. 1 used 352 male broilers (8 replicates of 22 birds) and a low-viscosity normal hulled barley, while Exp. 2 utilized 484 female broilers (11 replicates of 22 birds) and a normal hulled cultivar of high-viscosity. All diets in both trials were supplemented with phytase and a xylanase and β-glucanase complex. In Exp. 1, amylase tended to improve feed conversion ratio (P &lt; 0.1) and increased AID of crude protein (CP) (P &lt; 0.05) at 33 d of age but had no effect on AID of starch. In Exp. 2, no effects of amylase were observed on growth performance, AID of starch and CP, or relative organ weights. Overall findings from both Exp indicate that amylase supplementation can increase CP digestibility of barley-based diets depending on intrinsic viscosity, which may then improve performance. High starch digestibility observed in diets without amylase explains the lack of enzyme effect and suggests the investigated barleys caused no major detrimental effects to nutrient utilization, although the use of high-viscosity barley in Exp. 2 led to lower AID of starch and CP than Exp. 1. The use of a fiber-degrading enzyme likely enhanced starch utilization, leaving limited room for improvements with amylase for broilers at this age.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"330 ","pages":"Article 116568"},"PeriodicalIF":2.7,"publicationDate":"2025-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145569046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of heat-killed Lactobacillus plantarum (HK L-137) and propionic acid on growth and health of striped catfish (Pangasianodon hypophthalmus)","authors":"Pham Minh Duc ,&nbsp;Tran Thi Thanh Hien ,&nbsp;Tran Thi Tuyet Hoa ,&nbsp;Tran Ngoc Tuan","doi":"10.1016/j.anifeedsci.2025.116567","DOIUrl":"10.1016/j.anifeedsci.2025.116567","url":null,"abstract":"<div><div>Heat-killed <em>Lactobacillus plantarum</em> (HK L-137) is known to benefit striped catfish (<em>Pangasianodon hypophthalmus</em>) health, but its synergistic potential with short-chain fatty acids remains unexplored. This study investigated the individual and combined effects of HK L-137 and propionic acid (PA) on growth performance, feed utilization, immune responses, and stress response in striped catfish. Striped catfish (n = 8400, mean body mass 4.09 ± 0.08 g) were randomly assigned to six feeding treatments with different levels of HK L-137 and PA (0 [control], 1000 mg PA/kg feed [PA.1000], 2 mg HK L-137/kg feed [HK.2], 4 mg HK L-137/kg feed [HK.4], 2 mg HK L-137/kg feed + 100 mg PA/kg feed [HK.2 +PA.100], and 2 mg HK L-137/kg feed + 1000 mg PA/kg feed [HK.2 +PA.1000], for eight weeks (with four replicates for each treatment). Results showed that, compared to the control, HK.2 significantly enhanced growth, improved digestive enzyme activity (amylase, trypsin, and chymotrypsin), and reduced the feed conversion ratio (<em>P &lt; 0.05</em>). PA.1000 increased lymphocyte counts, while both HK.2 and HK.4 elevated cortisol and lipid peroxidase levels (<em>P &lt; 0.05</em>). Furthermore, catalase activity was significantly increased in HK.4 and HK.2 +PA.1000 groups (<em>P &lt; 0.05</em>). Notably, both HK.2 and HK.4 groups exhibited a reduced cortisol response following high-density crowding stress (3000 fish/m³). In conclusion, dietary HK L-137 effectively improved growth, feed efficiency, and digestive function, while also enhancing stress tolerance in striped catfish; however, the synergistic effects with PA were limited. These findings support the application of HK L-137 as a promising functional feed additive for striped catfish aquaculture.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"330 ","pages":"Article 116567"},"PeriodicalIF":2.7,"publicationDate":"2025-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145517356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating variables affecting Pellet Durability Index (PDI) in pelleted corn-soy-based feeds for swine and poultry: A meta-analysis","authors":"Jihao You ,&nbsp;Kendra Hall ,&nbsp;Jessica Civiero ,&nbsp;Mark C. Malpass ,&nbsp;Dan Tulpan ,&nbsp;Jennifer L. Ellis","doi":"10.1016/j.anifeedsci.2025.116566","DOIUrl":"10.1016/j.anifeedsci.2025.116566","url":null,"abstract":"<div><div>Pelleting is one of the most widely used feed processing methods in poultry and swine production systems, as it enhances feed handling, improves animal production efficiency, and reduces feed waste. However, poor pellet quality remains a significant challenge for swine and poultry feed manufacturing, limiting economic returns at both the mill and farm levels. Predicting and optimizing pellet quality can be challenging due to the numerous factors in the feed manufacturing process, including diet formulation, production parameters and environmental conditions. A meta-analysis of published studies was conducted to investigate and quantify the impact of formulation and manufacturing factors on pellet quality and develop predictive equations for Pellet Durability Index (PDI), which represents an in-mill metric for pellet quality. Following a systematic review of the literature, a dataset comprising 16 variables and 280 treatment mean observations was extracted from 29 published studies. Models were developed while treating the study as a random effect. Eight factors were selected for model development, including feed composition factors comprising ingredient inclusions and nutrient content as well as key manufacturing parameters. All models were assessed using a 3-fold cross-validation approach, by placing entire studies into folds. The results showed that the bivariable model using <em>Lipid (g/kg)</em> and <em>Protein (g/kg)</em>, resulted in the best PDI prediction performance, with a Concordance Correlation Coefficient of 0.57 ± 0.076, a bias correction factor of 0.91 ± 0.092, and a Pearson Correlation Coefficient of 0.63 ± 0.137. This meta-analysis provides not only a pipeline for selecting models based on a set of statistical criteria, but also produced simple models that can be utilized in feed manufacturing to estimate PDI when data availability is limited.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"330 ","pages":"Article 116566"},"PeriodicalIF":2.7,"publicationDate":"2025-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145517357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Use of microencapsulated pepper with different levels of energy supplementation in grazing beef cattle","authors":"Ana Paula Gonçalves Ferreira Miranda ,&nbsp;Gleidson Giordano Pinto de Carvalho ,&nbsp;Douglas dos Santos Pina ,&nbsp;Leandro Soares Santos ,&nbsp;Lara Maria Santos Brant ,&nbsp;Paula Velozo Leal ,&nbsp;Aureliano José Vieira Pires ,&nbsp;Raiane Barbosa Mendes ,&nbsp;Evandro Pereira Neto ,&nbsp;Mirella Matos de Oliveira Xavier ,&nbsp;Caio Seiti Takiya ,&nbsp;José Esler de Freitas Júnior","doi":"10.1016/j.anifeedsci.2025.116564","DOIUrl":"10.1016/j.anifeedsci.2025.116564","url":null,"abstract":"<div><div>The objective of this study was to evaluate the effects of microencapsulated hot chili pepper (MCP) and different energy supplementation levels on nutrient intake and digestibility, ingestive behavior, ruminal fermentation, nitrogen and energy balance, and physiological parameters of grazing Nellore bulls. Six rumen-cannulated Nellore bull (313 ± 31 kg body weight (BW)) were distributed in a double 3 × 3 Latin square design with a 3 × 2 factorial treatment arrangement and 21-day experimental periods (14 days of adaptation and 7 days of data collection). The animals were housed in a pen on Pangola grass pasture (<em>Digitaria decumbens</em>). Treatments consisted of three energy supplementation levels (SUP) (0.15, 0.30, and 0.45 % of BW) with or without MCP inclusion (450 mg/animal/day of Capsicum sp). MCP inclusion did not affect nutrient intake or digestibility (<em>P</em> &gt; 0.05). Increasing energy supplementation levels led to greater intake and digestibility of non-fiber carbohydrates (<em>P</em> &lt; 0.01) and reduced ruminal ammonia nitrogen concentrations (<em>P</em> &lt; 0.01). The inclusion of MCP increased rumen propionate concentration (<em>P</em> &lt; 0.01) and increased heart rate (<em>P</em> = 0.01). An interaction effect between SUP and MCP was observed for the C2:C3 ratio (<em>P</em> = 0.04). Animals fed 0.15 % SUP with MCP and 0.30 % SUP without MCP presented a higher ratio than those supplemented with 0.30 % MCP and 0.45 % with or without MCP. The inclusion of microencapsulated chili pepper in the diet of grazing beef cattle, combined with intermediate levels of energy supplementation, is a strategy to promote adjustments in ruminal fermentation and improve energy use efficiency.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"330 ","pages":"Article 116564"},"PeriodicalIF":2.7,"publicationDate":"2025-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145517327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}