Animal Feed Science and Technology最新文献

{"title":"Effects of organic microminerals (selenium, copper, and zinc) and mannan-oligosaccharides on the antioxidant and immune responses of Nile tilapia","authors":"Carolina Vasconcelos Tavares de Farias ,&nbsp;Thaise Mota Sátiro ,&nbsp;Amanda Miyuki Oshiro ,&nbsp;Amalia Pérez-Jiménez ,&nbsp;Leonardo Susumu Takahashi","doi":"10.1016/j.anifeedsci.2026.116633","DOIUrl":"10.1016/j.anifeedsci.2026.116633","url":null,"abstract":"<div><div>Individual use of organic trace minerals and mannan oligosaccharides promotes fish health. However, few studies have investigated their combined use, despite the possibility that these compounds may have additive or synergistic effects, resulting in greater benefits than their isolated application. Thus, we investigated the effect of Aquate Fish FT, a product combining selenium from yeast, peptide-chelated copper, peptide-chelated zinc, and mannan oligosaccharides, on gene expression related to the antioxidant and immune systems, liver antioxidant enzymes, leukocyte respiratory burst, total globulins, and growth performance in Nile tilapia after a four-week feeding period (Trial I). In addition, we evaluated the effects of Aquate Fish FT on liver antioxidant enzyme activities, leukocyte respiratory burst, total globulin levels, and growth performance after an eight-week feeding period (Trial II). In this second trial, fish were characterized before being subjected to an intraperitoneal challenge with endotoxin lipopolysaccharides extracted from <em>Escherichia coli</em>, administered at a dose of 500 µg kg⁻¹ in a volume of 0.2 mL per gram of fish, followed by sampling 24 h after the challenge. A total of 160 juvenile Nile tilapia (<em>Oreochromis niloticus</em>) (63.56 ± 4.41 g; mean ± SD) were fed isoproteic (30 % CP) and isoenergetic (4000 kcal GE kg⁻¹) diets containing four levels (0; 0.4; 0.8; and 1.2 %) of Aquate Fish FT and four repetition. The results showed that after four weeks, Aquate Fish FT promoted enhanced antioxidant and immune defenses, evidenced by upregulation of catalase (CAT) gene expression (P = 0.008), increased hepatic superoxide dismutase (SOD) enzyme activity (P = 0.021), upregulation of the immune cytokine interleukin 1β (IL-1β) (P = 0.002), and elevated leukocyte respiratory activity (P = 0.030). No significant differences were observed in the expression of superoxide dismutase (SOD2) (P = 0.181), glutathione peroxidase (GPx) (P = 0.192), interleukin 8 (IL-8) genes (P = 0.067), total globulins (P = 0,137), and growth performance (P &gt; 0.05). After eight weeks, Aquate Fish FT significantly increased catalase (CAT) activity, with an even greater effect following lipopolysaccharide challenge (P = 0.012). Interestingly, leukocyte respiratory burst was reduced after lipopolysaccharide administration (P = 0.012), while total globulin levels increased (P = 0.028). In this trial, there was also no difference in growth performance (P &gt; 0.05). Therefore, our results indicate that the combination of organic trace minerals (selenium, copper, and zinc) and mannan oligosaccharides (Aquate Fish FT) appears to modulate antioxidant and immunological parameters in Nile tilapia throughout different feeding periods.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"334 ","pages":"Article 116633"},"PeriodicalIF":2.7,"publicationDate":"2026-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145976107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of corn gluten meal and fermented corn gluten meal supplementation on growth and physiological responses in Holstein dairy calves","authors":"Amir Hossein Sarzaeim ,&nbsp;Mahdi Ganjkhanlou ,&nbsp;Abolfazl Zali ,&nbsp;Mehdi Dehghan Banadaky ,&nbsp;Ashkan Fekri ,&nbsp;Lucrezia Forte ,&nbsp;Valiollah Palangi ,&nbsp;Pasquale De Palo ,&nbsp;Aristide Maggiolino","doi":"10.1016/j.anifeedsci.2026.116636","DOIUrl":"10.1016/j.anifeedsci.2026.116636","url":null,"abstract":"<div><div>This study evaluated the effects of incorporating corn gluten meal (CGM) and fermented corn gluten meal (FCGM) into calf starter diets on growth performance, nutrient utilization, ruminal fermentation, feeding behavior, and blood metabolites in pre-weaned Holstein calves. Thirty calves were randomly assigned to three dietary treatments: control (CON, 100 % calf starter), CGM (5 % inclusion, replacing an equal proportion of starter on a DM basis), and FCGM (5 % inclusion, DM basis). Outcomes were monitored from day 3 to day 60 of life. Data were analyzed using mixed-model ANOVA to assess diet and time effects. Calves fed CON exhibited the highest dry matter and crude protein intake; however, calves receiving CGM and FCGM showed improved feed conversion ratios with comparable average daily gains, indicating enhanced efficiency, particularly in the FCGM group. Relative to CON, FCGM increased apparent total-tract dry matter (DM) digestibility. Ruminal fermentation profiles were modulated, with an increased acetate:propionate ratio in FCGM-fed calves. Plasma metabolite patterns were consistent with improved nitrogen utilization in the FCGM group. Feeding behavior was largely unaffected by diet. Collectively, a 5 % inclusion of FCGM in the starter improved growth efficiency and aspects of rumen function over days 3–60 of life, without compromising intake, supporting FCGM as a viable early-life nutritional strategy.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"333 ","pages":"Article 116636"},"PeriodicalIF":2.7,"publicationDate":"2026-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145973988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of a simplified in vitro method to estimate the rumen protein degradation","authors":"Gonzalo Fernández-Turren ,&nbsp;Claudio Antonio Pozo ,&nbsp;José Luis Repetto ,&nbsp;Cecilia Cajarville ,&nbsp;Gilberto Vilmar Kozloski","doi":"10.1016/j.anifeedsci.2026.116634","DOIUrl":"10.1016/j.anifeedsci.2026.116634","url":null,"abstract":"<div><div>This study aimed to evaluate the reliability of a simplified method to estimate the ruminal degradability of crude protein (CP), based on ammonia N release <em>in vitro</em>, compared to the <em>in situ</em> conventional method. Two preliminary trials were conducted to evaluate the dynamics of ammonia N production and uptake by rumen microorganisms and its relationship with gas and purine bases (PB) accumulation <em>in vitro</em> as an indicator of microbial biomass. In Trial 1, starch, cellulose and casein samples were incubated and gas production and both ammonia N and PB accumulation were measured. No significant correlation was found between gas, ammonia N and PB accumulation. In Trial 2, casein and soybean meal samples were incubated with rumen inoculum added with 10 mg/L of (¹⁵NH₄)₂SO₄ and gas production, ammonia N accumulation and ¹⁵N concentration in the incubation medium were measured. Ammonia N uptake represented only 6 % of the total ammonia N released throughout the 48 h of fermentation of casein or soybean meal. The ¹⁵N concentration drastically decreased up to 24 h of incubation. Trial 3 evaluated whether the <em>in vitro</em> CP degradation parameters of ten moderate to high-protein feedstuffs were related to those obtained by the <em>in situ</em> conventional method. There was a positive linear relationship (<em>P</em> &lt; 0.05) between methods for estimating the potentially degradable CP (R² ≥ 0.80) and the fractional rate of CP degradation (<em>kd</em>; R² = 0.56), with the <em>in vitro kd</em> being, on average, 66 % lower than the <em>in situ.</em> Effective CP degradability (ECPD) of feedstuffs estimated in both methods was positively related (R² = 0.93; <em>P</em> &lt; 0.05). The simplified method based on ammonia N release <em>in vitro</em>, without correction for ammonia N uptake by microorganisms, may be used to estimate the extent or ruminal protein degradation of feedstuffs with moderate to high CP content.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"333 ","pages":"Article 116634"},"PeriodicalIF":2.7,"publicationDate":"2026-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145973992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of a water-based delivery of an Ascophyllum nodosum extract on animal performance, rumen microbiota, fermentation, and methane emissions of zero-grazed beef steers","authors":"Aaron Casey ,&nbsp;Tommy M. Boland ,&nbsp;Alan K. Kelly ,&nbsp;Zoe C. McKay ,&nbsp;Fionnuala Godwin ,&nbsp;Maria Markiewicz-Keszycka ,&nbsp;Marco Garcia-Vaquero ,&nbsp;Calum Bridson ,&nbsp;Padraig Hennessy ,&nbsp;Sivaprakash Balakrishnan ,&nbsp;Kieran J. Guinan ,&nbsp;John T. O’Sullivan ,&nbsp;Stafford Vigors","doi":"10.1016/j.anifeedsci.2026.116632","DOIUrl":"10.1016/j.anifeedsci.2026.116632","url":null,"abstract":"<div><div>This study evaluated the effects of an <em>Ascophyllum nodosum</em> (ASC) extract delivered via a water-based system on animal performance, rumen microbiota, fermentation, and methane (CH₄) emissions in beef steers. Thirty dairy-beef steers were randomly allocated to one of two dietary treatments (<em>n</em> = 15) for a 70-d period. Treatments consisted of either <em>ad libitum</em> zero-grazed grass (<strong>CTR</strong>; no extract) or <em>ad libitum</em> zero-grazed grass plus an ASC extract (<strong>ASCE</strong>) supplemented at 1.5 % of dry matter intake (DMI), administered via a water-based system. Additionally, all steers were offered a maximum of 1.4 kg/d of concentrate. Methane was measured throughout the study using the GreenFeed emissions monitoring system (GEM). A feed intake estimation period was conducted during week 5 and 10, while rumen fluid samples were collected on d-33 and d-68 for rumen microbiota and fermentation analysis. There was no difference between treatments for total DMI, average daily gain (ADG), or diet digestibility. Methane production was similar between treatments, averaging 206.6 g/d for CTR and 204.6 g/d for ASCE. Methane yield (g/kg DMI) and intensity (g/kg ADG) were also unaffected, averaging 24.8 g/kg DMI and 130.8 g/kg ADG, respectively. The proportion of butyrate was lower, and ammonia-nitrogen (NH₃-N) concentration was higher in ASCE-treated steers. Microbial abundances were unaffected, with <em>Bacteroidota</em> (51 %) and <em>Firmicutes</em> (38.3 %) being the most abundant phyla across both treatments. In conclusion, ASCE supplementation did not impact animal performance, CH₄ emissions or rumen microbiota.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"333 ","pages":"Article 116632"},"PeriodicalIF":2.7,"publicationDate":"2026-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145940318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of guanidinoacetic acid and methionine supplementation in low-forage diets on ruminal fermentation and performance of feedlot-finished steers","authors":"Nathalia Marques Andriotti,&nbsp;Wagner Paris,&nbsp;Fábio José Maia,&nbsp;Ruan Carlos Castilho Martins,&nbsp;Otávio Fancé,&nbsp;Laura Carpenedo Pinto,&nbsp;Luís Fernando Glasenapp de Menezes","doi":"10.1016/j.anifeedsci.2025.116631","DOIUrl":"10.1016/j.anifeedsci.2025.116631","url":null,"abstract":"<div><div>Feed additives that improve energy metabolism efficiency can enhance cattle performance and nitrogen use. This study evaluated the effects of guanidinoacetic acid (GAA) and methionine (MET) supplementation, alone or combined, on performance, ruminal parameters, and nitrogen metabolism of feedlot-finished steers. Forty Angus steers (20 ± 2 months old; initial body weight 431.9 ± 8.9 kg) were randomly allocated to individual pens and fed for 86 days (plus 20-day adaptation period to the diets, totaling 106 days of feedlot). Feed intake, rumen, urine and blood data were collected in three experimental periods to assess responses over time. Animals were distributed in a completely randomized 2 × 2 factorial design (n = 10 per treatment): Control, GAA (6 g/d), MET (5 g/d), and GAA + MET. Data were analyzed using ANOVA followed by Tukey’s test (P &lt; 0.05) to evaluate main effects and interactions. No significant effects of GAA, MET, or their interaction were observed on average daily gain or dry matter intake (P &gt; 0.05). GAA supplementation increased total ruminal volatile fatty acid (VFA) concentration (57.3 vs. 46.5 mmol/L) and tended to alter acetate proportion, indicating enhanced fermentation efficiency. Nitrogen excretion and plasma metabolites were unaffected. GAA altered ruminal fermentation patterns but did not improve performance traits under the conditions of this high-energy finishing diet. This research contributes to understanding how GAA affects rumen fermentation and nitrogen metabolism in feedlot cattle, supporting more precise use of energy precursors in nutritional strategies.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"333 ","pages":"Article 116631"},"PeriodicalIF":2.7,"publicationDate":"2025-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145940317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary soybean isoflavones modulate reproductive hormones and immunity by altering rumen microbiota in ewes","authors":"Mengqi Lou ,&nbsp;Xiongxiong Li ,&nbsp;Shengguo Zhao ,&nbsp;Jinlong Xu ,&nbsp;Qingshan Fan ,&nbsp;Yuzhu Sha ,&nbsp;Jie Bai ,&nbsp;Ting Jiao","doi":"10.1016/j.anifeedsci.2025.116618","DOIUrl":"10.1016/j.anifeedsci.2025.116618","url":null,"abstract":"<div><div>Soybean isoflavones may improve reproductive performance in ruminants by modulating rumen microbiota. We hypothesized that dietary soybean isoflavone (SI) supplementation would alter reproductive hormone profiles, antioxidant capacity, and immunity in ewes through rumen microbial alterations. Twenty Hu sheep ewes (1.5 months old; 17.82 ± 0.98 kg BW) received either a basal diet (CON) or a basal diet + 30 mg SI/kg BW throughout estrus (the day after completion of estrus synchronization treatment), pregnancy (on day 120 of gestation, with all ewes confirmed pregnant), and lactation (on day 30 of postpartum). The reproductive performance of the ewes was recorded, and the body weight of newborn lambs was measured at 15 - day intervals. Ewes blood and rumen fluid samples were analyzed for hormones, antioxidants, immune parameters, fermentation products, and microbial diversity via 16S rRNA sequencing. Although no significant changes were observed on ewes reproductive performance and the newborn lambs birth weight, SI supplementation increased (<em>P</em> &lt; 0.05) reproductive hormones (E2, FSH, LH, P4) across most reproductive stages compared with CON. Antioxidant enzymes (GSH-Px, CAT) increased (<em>P</em> &lt; 0.05) while malondialdehyde decreased during critical periods. Immunoglobulins (IgA, IgG, IgM) were enhanced (<em>P</em> &lt; 0.05) throughout the reproductive cycle. SI increased ruminal acetate and propionate during lactation and altered microbial composition, reducing the abundance of <em>unclassified_Clostridia</em> and <em>Lachnospiraceae_XPB_1014_group</em> during lactation (<em>P</em> &lt; 0.05). Correlation analysis revealed significant associations between altered microbial genera and improved physiological parameters. The results indicate that SI supplementation can modulate reproductive hormones profiles in ewes, enhance antioxidant defense capacity and immune function by modulating rumen microbiota, supporting its application as a natural feed additive in sheep farming.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"333 ","pages":"Article 116618"},"PeriodicalIF":2.7,"publicationDate":"2025-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145940316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of hydroxy methionine zinc on growth performance, digestive enzyme activity, antioxidant, and immune competence of Australian red claw crayfish (Cherax quadricarinatus)","authors":"De-Hua Kong ,&nbsp;Yu-Xiang Ji ,&nbsp;Bao-Yuan Zhang ,&nbsp;Zi-Yan Liao ,&nbsp;Jing-Xiang Zhou ,&nbsp;Qiu-Ju Wang","doi":"10.1016/j.anifeedsci.2025.116624","DOIUrl":"10.1016/j.anifeedsci.2025.116624","url":null,"abstract":"<div><div>Zinc is an essential trace element in living organisms, which is recognized as the “life element”, hydroxy methionine zinc (Zn-MHA), as a fourth-generation zinc product, exhibits advantages such as well-defined and stable structure, easy absorption, and broad application potential in aquatic species. The current study assesses the efficacy of Zn-MHA in <em>Cherax quadricarinatus</em>. This breeding experiment was conducted in the greenhouse of Gongzhuling Rice and Fishing Base, Jilin Province, China. Five experimental diets were formulated by supplementing a basal diet with 0 (control, C0), 15 (Z1), 30 (Z2), 60 (Z3), and 90 (Z4) mg kg⁻¹ of Zn-MHA. Crayfish with an initial body mass of 0.50 ± 0.01 g were selected and fed for 8 weeks. The experimental results show that the group fed the zinc-free diet exhibited inferior growth performance, digestive enzyme activity, and antioxidant and immune-related indices compared to the zinc-supplemented groups. These results indicate that dietary Zn-MHA supplementation at 15–90 mg kg⁻¹ promotes growth, improves digestive function, and enhances antioxidant and immune capacities in red claw crayfish. Under the experimental conditions, the optimal dosage was determined to be 60 mg kg⁻¹. This can providing a scientific basis for its rational application in feed formulations.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"333 ","pages":"Article 116624"},"PeriodicalIF":2.7,"publicationDate":"2025-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145940315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Economic and productive impacts of dietary turnip (Brassica rapa) leaf powder on Nile tilapia (Oreochromis niloticus) fingerlings","authors":"Samia Fawzy ,&nbsp;Sara Mohammed ,&nbsp;Haitham G. Abo-Al-Ela ,&nbsp;Mohamed A. Helal ,&nbsp;Seham El-Kassas","doi":"10.1016/j.anifeedsci.2025.116622","DOIUrl":"10.1016/j.anifeedsci.2025.116622","url":null,"abstract":"<div><div>The present study investigated the impact of the dietary inclusion of dried turnip leaf (DTL) powder on growth performance, hematological and immunological parameters, serum biochemistry, antioxidant and digestive enzyme activity, gut health and economic efficiency indices in mono-sex Nile tilapia. A total of 225 mono-sex Nile tilapia (<em>Oreochromis niloticus</em>) with an average weight of 2.13 ± 0.5 g were randomly assigned to five treatments, with three replicates each resulting in 15 glass aquaria (each containing 15 fingerlings). The DTL powder was included at 0, 2, 4, 6, and 8 % of the diet for DTL0, DTL2, DTL4, DTL6, and DTL8, respectively, and the experiment lasted for 65 days. The chemical analysis of DTL revealed several compounds, with <em>p</em>-coumaric acid, ferulic acid, chlorogenic acid, and caffeic acid present at the highest levels. The results revealed a significant improvement in Nile tilapia growth performance and feed utilization, as confirmed by increases in final weight, body weight gain, SGR, and PER and improvements in FCR, with a peak of 6 % DTL. The improved growth performance was correlated with enhanced gut health manifested by improved intestinal histomorphological features, reducing the total bacterial count with a favorable shift to beneficial <em>Lactobacillus</em> (<em>P</em> &lt; 0.05). Furthermore, the dietary inclusion of DTL powder markedly increased hematological indices (<em>P</em> &lt; 0.05), including the levels of antioxidants (SOD and CAT), accompanied by a reduction in the phagocytic activity of MDA and lysozyme activity without altering the serum lipid profile (<em>P</em> &lt; 0.001). Profitability and economic efficiency were also enhanced in all the DTL-containing groups, peaking at 6 % DTL (<em>P</em> ≤ 0.001). Therefore, the dietary incorporation of up to 6 % DTL powder could be a cost-effective alternative feed ingredient to increase the growth, feed utilization, general health, and immunity of Nile tilapia.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"333 ","pages":"Article 116622"},"PeriodicalIF":2.7,"publicationDate":"2025-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145883168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Easy, on-site applicable DNA extraction in combination with LAMP analysis for the early detection of DNA derived from processed animal proteins in poultry feed","authors":"Marleen M. Voorhuijzen-Harink ,&nbsp;Vittorio Saggiomo ,&nbsp;Bas J. Fronen ,&nbsp;Theo W. Prins ,&nbsp;Valerie van der Vorst ,&nbsp;Toine F.H. Bovee ,&nbsp;Aldrik H. Velders ,&nbsp;Gert IJ. Salentijn","doi":"10.1016/j.anifeedsci.2025.116616","DOIUrl":"10.1016/j.anifeedsci.2025.116616","url":null,"abstract":"<div><div>The use of processed animal proteins (PAPs) has been re-authorized in feed materials, but the ban on cannibalism is still in place. Food safety authorities perform controls in feed-producing factories, but send limited numbers of samples to official control laboratories, as current methods are time-consuming. Food safety control would, therefore, benefit from early on-site identification of potentially high-risk sample streams with a higher chance of intra-species cross-contamination of the produced feed. In addition, feed producers would benefit from simple assays to check their (ingredient or semi-) materials for potentially fraudulent admixtures or contaminations. To enable fast detection and to address both costs and speed, we developed easy-to-use, on-site applicable loop-mediated amplification (LAMP) assays for the detection of poultry (i.e. chicken, turkey, ducks, geese, and helmeted guineafowl as defined by the European Commission) in highly processed feed materials. Additionally, to demonstrate applicability on-site, the developed DNA-based LAMP assays were combined with easy-to-use DNA extraction and amplification devices, resulting in an entire workflow of less than one hour. The performance of the developed assays was evaluated by testing fresh and processed meat mixtures spiked with poultry meat, and highly processed poultry feed materials spiked with poultry PAPs. A comparison of LAMP with the EURL-AP recommended laboratory qPCR method showed that LAMP performs similarly as qPCR regarding the detection of spiked chicken or turkey meat in fresh meat mixtures, namely 0.1 %, but qPCR was found to be slightly more sensitive for the detection of poultry PAPs in highly processed feed materials.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"333 ","pages":"Article 116616"},"PeriodicalIF":2.7,"publicationDate":"2025-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145847626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling the mechanism by which Lactiplantibacillus plantarum modulates alfalfa silage quality and antioxidant phytochemical accumulation through integrated microbiome–metabolomics analysis","authors":"Yuqian Liu ,&nbsp;Ze Wang ,&nbsp;Wenting Gou ,&nbsp;Jia Fu ,&nbsp;Mengxue Liu ,&nbsp;Nanyi Zhang ,&nbsp;Qiang Si ,&nbsp;Hongmei Shang","doi":"10.1016/j.anifeedsci.2025.116621","DOIUrl":"10.1016/j.anifeedsci.2025.116621","url":null,"abstract":"<div><div>Investigating the effects of different <em>Lactiplantibacillus plantarum</em> additives on silage quality and metabolic pathways is highly important for ensuring livestock product safety. This study examined how <em>Lactiplantibacillus plantarum</em> strains LP1 and H8 influence alfalfa silage quality, fermentation characteristics, antioxidant activity, and microbial communities over ensiling periods of 6, 15, 30, 45, and 60 days. The addition amount of <em>Lactiplantibacillus plantarum</em> was 1 × 10⁶ CFU/g fresh weight, and an equal volume of distilled water was added to the control group. After 60 days of ensiling, microbiome and metabolome analyses were performed to explore their potential functions and mechanistic contributions to the silage fermentation process. The <em>Lactiplantibacillus plantarum</em>-treated silage presented significantly (<em>P</em> &lt; 0.05) lower pH and ammonia nitrogen levels but greater preservation of crude protein, along with increased lactic acid, total phenolic, and flavonoid contents and radical-scavenging activity, indicating superior nutrient conservation in the alfalfa silage. Microbiome analysis revealed the bacterial community structure of alfalfa silage, demonstrating that <em>Lactiplantibacillus plantarum</em>-inoculated silage was characterized by a relatively high abundance of <em>Lactiplantibacillus</em> and the effective inhibition of undesirable microorganisms (<em>Stenotrophomonas</em> and <em>Chryseobacterium</em>). Notably, metabolomics analysis confirmed significant metabolic alterations in <em>Lactiplantibacillus plantarum</em>-inoculated alfalfa silage. The treatment markedly increased the contents of flavonoid compounds, including (-)-epigallocatechin, (+)-catechin, farrerol, and apigenin. Correlation analysis revealed <em>Lactobacillus</em> and <em>Sphingomonas</em> as key microbial drivers of antioxidant metabolite production in <em>Lactiplantibacillus plantarum</em>-treated silage. Overall, <em>Lactiplantibacillus plantarum</em> inoculation profoundly influences silage quality, microbial composition, and metabolic pathways by increasing the bacterial abundance and flavonoid content, thereby improving fermentation characteristics and antioxidant capacity.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"332 ","pages":"Article 116621"},"PeriodicalIF":2.7,"publicationDate":"2025-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145837713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}