Objective: In this study, it was aimed to perform in silico studies on the papain-like protease structure of SARS-CoV-2 (PDB: 7JIT) of 1300 FDA-approved drugs downloaded from the ZINC database. Material and Method: A molecular docking study was performed with PLpro (PDB ID: 7JIT) using four different molecular docking programs for a total of 1300 FDA-approved drugs obtained from the ZINC database. Conivaptan and amphotericin B were obtained in docking analysis with AutoDock Vina and Sybyl-X, respectively. Docking analysis with Glide SP and Glide XP resulted in fludarabine and panobinostat, respectively. Molecular dynamics simulations were performed for a period of 120 ns to check the stability of these four drugs. Result and Discussion: The reliability of the results obtained using four different molecular docking programs on the SARS-CoV-2 papain-like protease of 1300 drug molecules was checked by reinserting the co-crystal ligand. Protein-ligand interactions between fludarabine, conivaptan, amphotericin-B, panobinostat, and PLpro were given. In the molecular dynamics study, RMSD, RMSF, Rg, and SASA analyses were performed for four systems. It was observed that RMSD remained constant for all 120 ns for all four systems except for amphotericin B, which deviated slightly towards the end of 120 ns. No significant fluctuation was noticed in the RMSF graphics for all four systems.
{"title":"IN SILICO EVALUATION OF SARS-COV-2 PAPAIN-LIKE PROTEASE INHIBITORY ACTIVITY OF SOME FDA-APPROVED DRUGS","authors":"Meryem Erol","doi":"10.33483/jfpau.1311496","DOIUrl":"https://doi.org/10.33483/jfpau.1311496","url":null,"abstract":"Objective: In this study, it was aimed to perform in silico studies on the papain-like protease structure of SARS-CoV-2 (PDB: 7JIT) of 1300 FDA-approved drugs downloaded from the ZINC database.\u0000Material and Method: A molecular docking study was performed with PLpro (PDB ID: 7JIT) using four different molecular docking programs for a total of 1300 FDA-approved drugs obtained from the ZINC database. Conivaptan and amphotericin B were obtained in docking analysis with AutoDock Vina and Sybyl-X, respectively. Docking analysis with Glide SP and Glide XP resulted in fludarabine and panobinostat, respectively. Molecular dynamics simulations were performed for a period of 120 ns to check the stability of these four drugs.\u0000Result and Discussion: The reliability of the results obtained using four different molecular docking programs on the SARS-CoV-2 papain-like protease of 1300 drug molecules was checked by reinserting the co-crystal ligand. Protein-ligand interactions between fludarabine, conivaptan, amphotericin-B, panobinostat, and PLpro were given. In the molecular dynamics study, RMSD, RMSF, Rg, and SASA analyses were performed for four systems. It was observed that RMSD remained constant for all 120 ns for all four systems except for amphotericin B, which deviated slightly towards the end of 120 ns. No significant fluctuation was noticed in the RMSF graphics for all four systems.","PeriodicalId":7891,"journal":{"name":"Ankara Universitesi Eczacilik Fakultesi Dergisi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43449432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ö. Çi̇ftçi̇, T. T. Şahi̇n, Hande Yüce, Sezai Yilmaz, Neşe BAŞAK TÜRKMEN, Ş. Yaşar, Tülay Çoban, Songül Ünüvar
Objective: Liver transplantation using a living donor (LDLT) is currently the most popular method used in the worldwide. Appropriate biomarkers that predict graft status should be used to detect early post-transplant complications that may lead to a rejection reaction. Material and Method: The study involved a total of 44 liver recipients and 44 liver donors, from whom preoperative blood samples were taken and immunoassay and spectrophotometric studies were carried out. The levels of serum neopterin, interferon-gamma (IFN- y), indoleamine-2,3 dioxygenase (IDO), and -glutathione S transferase (a-GST) were assessed using an enzyme-linked immunosorbent test. The activity of glutathione reductase (GR) and glucose-6-phosphate dehydrogenase (G6PD) in erythrocytes was measured using spectrophotometry. Result and Discussion: “Neopterin, IDO, and G6PD levels were significantly higher in the recipient group than in the donor group. The differences in gender and blood groups were statistically insignificant. The rejection reaction developed in 25% of patients and none survived. These findings may facilitate the identification of novel predictive biomarkers for the diagnosis of acute rejection reactions after LDLT. The clinical use of novel non-invasive biomarkers may provide time and cost advantages.
{"title":"CANLI DONÖR KARACİĞER NAKLİNİN ÖNGÖRÜSEL BİYOBELİRTEÇLERİ","authors":"Ö. Çi̇ftçi̇, T. T. Şahi̇n, Hande Yüce, Sezai Yilmaz, Neşe BAŞAK TÜRKMEN, Ş. Yaşar, Tülay Çoban, Songül Ünüvar","doi":"10.33483/jfpau.1247781","DOIUrl":"https://doi.org/10.33483/jfpau.1247781","url":null,"abstract":"Objective: Liver transplantation using a living donor (LDLT) is currently the most popular method used in the worldwide. Appropriate biomarkers that predict graft status should be used to detect early post-transplant complications that may lead to a rejection reaction. \u0000Material and Method: The study involved a total of 44 liver recipients and 44 liver donors, from whom preoperative blood samples were taken and immunoassay and spectrophotometric studies were carried out. The levels of serum neopterin, interferon-gamma (IFN- y), indoleamine-2,3 dioxygenase (IDO), and -glutathione S transferase (a-GST) were assessed using an enzyme-linked immunosorbent test. The activity of glutathione reductase (GR) and glucose-6-phosphate dehydrogenase (G6PD) in erythrocytes was measured using spectrophotometry.\u0000Result and Discussion: “Neopterin, IDO, and G6PD levels were significantly higher in the recipient group than in the donor group. The differences in gender and blood groups were statistically insignificant. The rejection reaction developed in 25% of patients and none survived. These findings may facilitate the identification of novel predictive biomarkers for the diagnosis of acute rejection reactions after LDLT. The clinical use of novel non-invasive biomarkers may provide time and cost advantages.","PeriodicalId":7891,"journal":{"name":"Ankara Universitesi Eczacilik Fakultesi Dergisi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47461758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: Using cyclic (CV) and differential pulse (DPV) voltammetric techniques, the electrochemical research of otilonium bromide (OTB) was carried out over a wide pH range (0.3–12) at glassy carbon electrodes (GCE) and boron-doped diamond electrodes (BDDE). The typical electrochemical behavior of OTB was identified as being dependent on the type of working electrode and pH. This research aims to provide a brand-new electroanalytical technique for measuring OTB in buffer solutions. Material and Method: All experiments employed the typical three-electrode cell of 10 ml capacity in conjunction with a platinum wire counter electrode, a BDDE and GCE working electrode, and an Ag/AgCl reference electrode. NOVA 1.8 software and an AUTOLAB 204 potentiostat/galvanostat were used for electrochemical measurements. Result and Discussion: The electrochemical behavior of OTB, which belongs to a class of drugs called 'antispasmodics' (spasm and cramps reliever), primarily used to treat irritable bowel syndrome (IBS), and other gastrointestinal conditions characterized by motility problems, painful bowel spasms and distension (swelling and bloating in the belly area), was examined in 0.1 M H2SO4 at BDDE and GCE. The electrooxidation mechanism was also investigated by conducting CV investigations at various pH levels throughout a broad pH range (pH 0.3-12.0). Understanding the mechanism was aided by scan rate investigations, which revealed that diffusion was controlled for both electrodes. The proposed technique was successfully used to determine OTB under optimal conditions.
{"title":"ELECTROCHEMICAL INVESTIGATION OF OTILONIUM BROMIDE USING BORON-DOPED DIAMOND AND GLASSY CARBON ELECTRODES","authors":"Leyla Karadurmus, E. B. Atici, S. Özkan","doi":"10.33483/jfpau.1344014","DOIUrl":"https://doi.org/10.33483/jfpau.1344014","url":null,"abstract":"Objective: Using cyclic (CV) and differential pulse (DPV) voltammetric techniques, the electrochemical research of otilonium bromide (OTB) was carried out over a wide pH range (0.3–12) at glassy carbon electrodes (GCE) and boron-doped diamond electrodes (BDDE). The typical electrochemical behavior of OTB was identified as being dependent on the type of working electrode and pH. This research aims to provide a brand-new electroanalytical technique for measuring OTB in buffer solutions.\u0000Material and Method: All experiments employed the typical three-electrode cell of 10 ml capacity in conjunction with a platinum wire counter electrode, a BDDE and GCE working electrode, and an Ag/AgCl reference electrode. NOVA 1.8 software and an AUTOLAB 204 potentiostat/galvanostat were used for electrochemical measurements.\u0000Result and Discussion: The electrochemical behavior of OTB, which belongs to a class of drugs called 'antispasmodics' (spasm and cramps reliever), primarily used to treat irritable bowel syndrome (IBS), and other gastrointestinal conditions characterized by motility problems, painful bowel spasms and distension (swelling and bloating in the belly area), was examined in 0.1 M H2SO4 at BDDE and GCE. The electrooxidation mechanism was also investigated by conducting CV investigations at various pH levels throughout a broad pH range (pH 0.3-12.0). Understanding the mechanism was aided by scan rate investigations, which revealed that diffusion was controlled for both electrodes. The proposed technique was successfully used to determine OTB under optimal conditions.","PeriodicalId":7891,"journal":{"name":"Ankara Universitesi Eczacilik Fakultesi Dergisi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46034928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: Mycotoxins are known as secondary fungal metabolites that cause biochemical, physiological, and/or pathological changes in many species, including animals and plants. According to the Food and Agriculture Organization (FAO), these substances contaminate about 25% of all food in the world. Mycotoxins are responsible for many different disorders affecting the gastrointestinal, urogenital, vascular, renal, and nervous systems, as well as cancers. Key strategies for preventing and controlling exposure to mycotoxins include controlling toxin formation, implementing surveillance and monitoring programs to prevent human exposure, detoxifying the mycotoxins through biological, chemical, and physical means, and promoting dietary diversification. Although exposure cannot be avoided entirely, implementing monitoring programs is crucial to minimize it. In general, prevention of exposure to mycotoxins should receive greater emphasis. This study aims to provide information about mycotoxins and raise awareness about mycotoxin exposure. Material and Method: This cross-sectional survey study was conducted with voluntary participants in Turkey between January 25, 2023-July 25, 2023, via electronic questionnaire. The questionnaire included socio-demographic data, income status, general dietary habits, frequency of consumption of mycotoxin-rich foods, knowledge about mycotoxins, and implementation of measures to prevent mycotoxin exposure. Result and Discussion: A total of 796 participants (52.6% female, 46.7% male, and 0.6% other) were included in the study. This study concludes that the participants considered both the price and quality of food to be important factors. The study indicates that a high percentage of participants are unaware of the concept of mycotoxin and the associated health risk. A statistically significant change was found between whether the participants were knowledgeable about nutrition and whether they knew the concept of mycotoxin (p<0.05). There was a statistically significant difference in participants' knowledge of healthy nutrition and knowledge of the health hazards of mycotoxins. (p<0.05). The results indicate a requirement for comprehensive management against mycotoxins and mycotoxin exposure, which can cause several health problems in humans.
{"title":"MİKOTOKSİNLERE DİYETLE MARUZİYETİ AZALTMAK İÇİN FARKINDALIK DÜZEYİ VE DAVRANIŞSAL ALIŞKANLIKLARININ DEĞERLENDİRİLMESİ","authors":"Göksu Demirel, Nida Nur Doğan","doi":"10.33483/jfpau.1344356","DOIUrl":"https://doi.org/10.33483/jfpau.1344356","url":null,"abstract":"Objective: Mycotoxins are known as secondary fungal metabolites that cause biochemical, physiological, and/or pathological changes in many species, including animals and plants. According to the Food and Agriculture Organization (FAO), these substances contaminate about 25% of all food in the world. Mycotoxins are responsible for many different disorders affecting the gastrointestinal, urogenital, vascular, renal, and nervous systems, as well as cancers. Key strategies for preventing and controlling exposure to mycotoxins include controlling toxin formation, implementing surveillance and monitoring programs to prevent human exposure, detoxifying the mycotoxins through biological, chemical, and physical means, and promoting dietary diversification. Although exposure cannot be avoided entirely, implementing monitoring programs is crucial to minimize it. In general, prevention of exposure to mycotoxins should receive greater emphasis. This study aims to provide information about mycotoxins and raise awareness about mycotoxin exposure.\u0000Material and Method: This cross-sectional survey study was conducted with voluntary participants in Turkey between January 25, 2023-July 25, 2023, via electronic questionnaire. The questionnaire included socio-demographic data, income status, general dietary habits, frequency of consumption of mycotoxin-rich foods, knowledge about mycotoxins, and implementation of measures to prevent mycotoxin exposure.\u0000Result and Discussion: A total of 796 participants (52.6% female, 46.7% male, and 0.6% other) were included in the study. This study concludes that the participants considered both the price and quality of food to be important factors. The study indicates that a high percentage of participants are unaware of the concept of mycotoxin and the associated health risk. A statistically significant change was found between whether the participants were knowledgeable about nutrition and whether they knew the concept of mycotoxin (p<0.05). There was a statistically significant difference in participants' knowledge of healthy nutrition and knowledge of the health hazards of mycotoxins. (p<0.05). The results indicate a requirement for comprehensive management against mycotoxins and mycotoxin exposure, which can cause several health problems in humans.","PeriodicalId":7891,"journal":{"name":"Ankara Universitesi Eczacilik Fakultesi Dergisi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47879105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ozge Ulker, A. Gökbulut, Nuran Coşkun, Pelin Kavas
Amaç: Kozmetik ürün, farklı kimyasal bileşiklerin bir kombinasyonu olup genellikle cilt koşullarını veya insan vücudunun kokusunu temizlemek ve iyileştirmek için kullanılır, güzelliği arttırır. AB'de, bitmiş kozmetik ürünler üzerinde hayvan testlerinin kullanılmasına ilişkin bir test yasağı 2004'ten beri yürürlükteyken, 2013'te deri hassasiyet testi de dahil olmak üzere her türlü hayvan toksisitesi testi için bir pazarlama yasağı yürürlüğe girmiştir. Gereç ve Yöntem: Deri hassasiyet testi için ECVAM tarafından üç farklı in-vitro ve bir kimyasal yöntem geliştirilmiş ve onaylanmıştır. Doğrudan Peptit Reaktivite Deneyi (DPRA) bir in-chemico yöntemidir. Testin amacı, kimyasalların deri hassasiyet potansiyelinin değerlendirilmesine katkıda bulunmaktır. Düşük moleküler ağırlıklı maddelerin (haptenler) ciltteki proteinlere kovalent bağlanması olan haptenizasyon, alerjide önemli bir mekanizma olarak kabul edilmektedir. Bu nedenle, DPRA gibi peptit reaktivite deneylerinden elde edilen bilgiler, kimyasalların deri hassasiyet potansiyelinin değerlendirilmesi hakkında bilgi sağlar. Çalışmamızda hassasiyet potansiyeli bilinen kimyasalları uygulanarak DPRA'yı kılavuza göre kurmak amaçlanmıştır. Sonuç ve Tartışma: Kozmetik bileşenlerin deri hassasiyet sınıflandırmasına yönelik DPRA çalışmamızın sonuçları, bu kimyasallar ile yapılan önceki in-vivo ve ex-vivo çalışmaların sonuçlarıyla uyumlu bulunmuştur. Çalışmaya eklenen ve kuvvetli irritasyon özelliğine sahip sodyum dodesil sülfat (SDS) uygulaması, DPRA yönteminin irritasyon ve alerji ayrımını yapabileceğini göstermesi açısından önem taşımaktadır. Daha güvenilir bir deri hassasiyet değerlendirmesi sağlamak için iki veya daha fazla yaklaşımdan elde edilen en iyi sonuçların nasıl birleştirileceği değerlendirilmelidir. Alternatif yöntemlerden elde edilen veriler, tek başına bir yöntem olarak kullanılmamalı, kanıt yaklaşımının ağırlıklı bir parçası olarak diğer bilgilerle birlikte değerlendirilmelidir. Bu nedenle, duyarlaştırıcı ve duyarlaştırıcı olmayan maddeler arasında ayrım yapmak mümkündür.
目标是将化妆品与不同的化学化合物结合起来，通常是为了清洁和改善人体的气味。在欧盟，自2004年起禁止动物毒性测试上市，包括2013年的皮肤敏感性测试。需要和方法：ECVAM开发并批准的三种不同的体外和化学方法用于皮肤敏感性测试。真正的肽反应体验（DPRA）是一种化学方法。该测试的目的是帮助评估化学品对皮肤的潜在敏感性。低分子被认为是过敏的主要机制，它将重物质的蛋白质结合到皮肤中。因此，从DPRA等消化性反应性实验中获得的信息提供了关于评估化学品的皮肤敏感性潜力的信息。Çalışmamızda hasasiyet potansiyeli bilinen kimyasalları维吾尔族DPRA'yıkılavuza göre kurmak amaçlanmı。结果和论点：DPRA化妆品成分皮肤敏感性类别的研究结果与之前的体内和离体研究结果一致。重要的是要表明，使用十二烷基硫酸钠（SDS）作为工作添加剂和强大的刺激特性会对DPRA方法造成刺激和过敏。为了评估更可靠的皮肤敏感性，应从两种或多种方法中获得的最佳结果来评估如何将它们结合起来。从备选方案中获得的数据不应作为单一方法使用，而应与其他信息一起评估，作为证据权重方法的一部分。这就是为什么可以区分传感器和非传感器。
{"title":"ASSESSMENT OF THE POTENTIAL OF ALLERGIC CONTACT DERMATITIS BY CHEMICALS BY DIRECT PEPTIDE REACTIVITY METHOD","authors":"Ozge Ulker, A. Gökbulut, Nuran Coşkun, Pelin Kavas","doi":"10.33483/jfpau.1274764","DOIUrl":"https://doi.org/10.33483/jfpau.1274764","url":null,"abstract":"Amaç: Kozmetik ürün, farklı kimyasal bileşiklerin bir kombinasyonu olup genellikle cilt koşullarını veya insan vücudunun kokusunu temizlemek ve iyileştirmek için kullanılır, güzelliği arttırır. AB'de, bitmiş kozmetik ürünler üzerinde hayvan testlerinin kullanılmasına ilişkin bir test yasağı 2004'ten beri yürürlükteyken, 2013'te deri hassasiyet testi de dahil olmak üzere her türlü hayvan toksisitesi testi için bir pazarlama yasağı yürürlüğe girmiştir. \u0000Gereç ve Yöntem: Deri hassasiyet testi için ECVAM tarafından üç farklı in-vitro ve bir kimyasal yöntem geliştirilmiş ve onaylanmıştır. Doğrudan Peptit Reaktivite Deneyi (DPRA) bir in-chemico yöntemidir. Testin amacı, kimyasalların deri hassasiyet potansiyelinin değerlendirilmesine katkıda bulunmaktır. Düşük moleküler ağırlıklı maddelerin (haptenler) ciltteki proteinlere kovalent bağlanması olan haptenizasyon, alerjide önemli bir mekanizma olarak kabul edilmektedir. Bu nedenle, DPRA gibi peptit reaktivite deneylerinden elde edilen bilgiler, kimyasalların deri hassasiyet potansiyelinin değerlendirilmesi hakkında bilgi sağlar. Çalışmamızda hassasiyet potansiyeli bilinen kimyasalları uygulanarak DPRA'yı kılavuza göre kurmak amaçlanmıştır.\u0000Sonuç ve Tartışma: Kozmetik bileşenlerin deri hassasiyet sınıflandırmasına yönelik DPRA çalışmamızın sonuçları, bu kimyasallar ile yapılan önceki in-vivo ve ex-vivo çalışmaların sonuçlarıyla uyumlu bulunmuştur. Çalışmaya eklenen ve kuvvetli irritasyon özelliğine sahip sodyum dodesil sülfat (SDS) uygulaması, DPRA yönteminin irritasyon ve alerji ayrımını yapabileceğini göstermesi açısından önem taşımaktadır. Daha güvenilir bir deri hassasiyet değerlendirmesi sağlamak için iki veya daha fazla yaklaşımdan elde edilen en iyi sonuçların nasıl birleştirileceği değerlendirilmelidir. Alternatif yöntemlerden elde edilen veriler, tek başına bir yöntem olarak kullanılmamalı, kanıt yaklaşımının ağırlıklı bir parçası olarak diğer bilgilerle birlikte değerlendirilmelidir. Bu nedenle, duyarlaştırıcı ve duyarlaştırıcı olmayan maddeler arasında ayrım yapmak mümkündür.","PeriodicalId":7891,"journal":{"name":"Ankara Universitesi Eczacilik Fakultesi Dergisi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47310537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sedef ÖZDEMİR KORKMAZ, M. A. Ege, Bintuğ Öztürk, Gözde ELGİN CEBE
Amaç: Bugüne kadar, tohumlu bitkilerin kimyasal içerikleri ve biyoaktiviteleri üzerine pek çok çalışma yapılmış olup, halen de yapılmaya devam edilmektedir. Ancak bu çalışmalar, farklı erişim kaynaklarında dağınık halde bulunmaktadır ve bu sebeple bilgiye erişim geç ya da eksik olabilmektedir. Bu nedenle bu çalışmada, Türkiye Florası tohumlu bitkileri üzerinde gerçekleştirilen biyoaktivite araştırmalarını kapsayan uzaktan erişilebilir, Türkçe ve İngilizce bir veritabanı oluşturularak bilgilerin sistematik olarak depolanması amaçlanmıştır. Gereç ve Yöntem: Çalışmanın ilk aşamasında Türkiye Florası tohumlu bitkileri üzerinde yapılmış ve 1928-2018 yılları arasında yayınlanmış biyoaktivite araştırmalarına ulaşılarak, bunlar incelenmiştir ve bir bibliyografya hazırlanmıştır. Çalışmanın ikinci aşamasında ise biyoaktivite çalışmalarının içeriğinde yer alan bilgilerin aktarılabilmesi için web tabanlı veritabanı yönetim sistemi geliştirilerek elde edilen literatürün ve içeriklerinin veritabanına kaydı yapılmıştır. Son olarak da veritabanına kaydedilen içeriklerin analizleri gerçekleştirilmiştir. Sonuç ve Tartışma: Yapılan taramalar sonucunda 1307 literatür bibliyografyaya dahil edilirken, bunların 1088’inin veritabanına kaydı uygun bulunmuş ve toplam 49.486 satır veri girişi yapılmıştır. Veriler detaylı incelendiğinde, 107 familyaya ait 430 cins ve 1594 taksonun biyoaktivite çalışmalarının kayıtlı olduğu görülmüştür. En çok taksonun yer aldığı familyalar ise Lamiaceae (307 takson), Asteraceae (271 takson) ve Fabaceae (125 takson) olarak belirlenmiştir. Veritabanına, 115 farklı aktivitenin kaydı yapılmıştır. En fazla sayıda taksonun; antimikrobiyal aktivite (962 takson), antioksidan aktivite (950 takson), sitotoksik aktivite (220 takson), antienflamatuar aktivite (160 takson) ve analjezik-antinosiseptif aktivite (113 takson) açısından araştırıldığı görülmektedir. En fazla biyoaktivite çalışmalarıının yapıldığı türler ise Urtica dioica (18 aktivite), Hypericum perforatum (17 aktivite) ve Cistus laurifolius (16 aktivite) olarak tespit edilmiştir. Lokalite kayıtları incelendiğinde en fazla taksonun İç Anadolu Bölgesinden, en az taksonun ise Güneydoğu Anadolu Bölgesinden temin edildiği saptanmıştır.
{"title":"BIOACTIVE SEED PLANTS DATABASE OF TURKEY","authors":"Sedef ÖZDEMİR KORKMAZ, M. A. Ege, Bintuğ Öztürk, Gözde ELGİN CEBE","doi":"10.33483/jfpau.1309030","DOIUrl":"https://doi.org/10.33483/jfpau.1309030","url":null,"abstract":"Amaç: Bugüne kadar, tohumlu bitkilerin kimyasal içerikleri ve biyoaktiviteleri üzerine pek çok çalışma yapılmış olup, halen de yapılmaya devam edilmektedir. Ancak bu çalışmalar, farklı erişim kaynaklarında dağınık halde bulunmaktadır ve bu sebeple bilgiye erişim geç ya da eksik olabilmektedir. Bu nedenle bu çalışmada, Türkiye Florası tohumlu bitkileri üzerinde gerçekleştirilen biyoaktivite araştırmalarını kapsayan uzaktan erişilebilir, Türkçe ve İngilizce bir veritabanı oluşturularak bilgilerin sistematik olarak depolanması amaçlanmıştır. \u0000Gereç ve Yöntem: Çalışmanın ilk aşamasında Türkiye Florası tohumlu bitkileri üzerinde yapılmış ve 1928-2018 yılları arasında yayınlanmış biyoaktivite araştırmalarına ulaşılarak, bunlar incelenmiştir ve bir bibliyografya hazırlanmıştır. Çalışmanın ikinci aşamasında ise biyoaktivite çalışmalarının içeriğinde yer alan bilgilerin aktarılabilmesi için web tabanlı veritabanı yönetim sistemi geliştirilerek elde edilen literatürün ve içeriklerinin veritabanına kaydı yapılmıştır. Son olarak da veritabanına kaydedilen içeriklerin analizleri gerçekleştirilmiştir.\u0000Sonuç ve Tartışma: Yapılan taramalar sonucunda 1307 literatür bibliyografyaya dahil edilirken, bunların 1088’inin veritabanına kaydı uygun bulunmuş ve toplam 49.486 satır veri girişi yapılmıştır. Veriler detaylı incelendiğinde, 107 familyaya ait 430 cins ve 1594 taksonun biyoaktivite çalışmalarının kayıtlı olduğu görülmüştür. En çok taksonun yer aldığı familyalar ise Lamiaceae (307 takson), Asteraceae (271 takson) ve Fabaceae (125 takson) olarak belirlenmiştir. Veritabanına, 115 farklı aktivitenin kaydı yapılmıştır. En fazla sayıda taksonun; antimikrobiyal aktivite (962 takson), antioksidan aktivite (950 takson), sitotoksik aktivite (220 takson), antienflamatuar aktivite (160 takson) ve analjezik-antinosiseptif aktivite (113 takson) açısından araştırıldığı görülmektedir. En fazla biyoaktivite çalışmalarıının yapıldığı türler ise Urtica dioica (18 aktivite), Hypericum perforatum (17 aktivite) ve Cistus laurifolius (16 aktivite) olarak tespit edilmiştir. Lokalite kayıtları incelendiğinde en fazla taksonun İç Anadolu Bölgesinden, en az taksonun ise Güneydoğu Anadolu Bölgesinden temin edildiği saptanmıştır.","PeriodicalId":7891,"journal":{"name":"Ankara Universitesi Eczacilik Fakultesi Dergisi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45370677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: The knowledge and attitude of pharmacists play an essential role in the patient counseling services of pharmacists. Celiac disease is one of the diseases in which patient follow-up and counseling are essential, and the number of studies dealing with the roles of pharmacists in this disease is very limited. This study aims to fill this gap and contribute to public health by evaluating community pharmacists' knowledge, attitudes, and practices for celiac disease. Material and Method: Based on the literature, a measurement tool including the knowledge, attitudes, and practices of community pharmacists for celiac disease has been developed. The measurement tool was applied online to community pharmacists in Türkiye in 2021. The obtained data were subjected to explanatory factor analysis (EFA). Result and Discussion: The number of pharmacists participating in this study is 408. A four-factor structure was obtained: knowledge of celiac disease, attitude towards celiac disease, counseling practices for celiac patients, and professional development practices. The Cronbach's alpha values of the factors were calculated between 0.794 and 0.935, which shows high reliability. These factors explained 70.343% of the total variance. The community pharmacists had positive attitudes toward counseling for celiac disease. Still, there were some deficiencies in terms of knowledge and practice. It is thought that the knowledge and awareness of pharmacists on celiac diseases can be increased by including issues related to celiac disease in both undergraduate education and vocational training programs.
{"title":"SERBEST ECZACILARIN ÇÖLYAK HASTALIĞINA YÖNELİK BİLGİ, TUTUM VE UYGULAMALARININ DEĞERLENDİRİLMESİ","authors":"Esin AKARSU ORUNÇ, M. Arslan","doi":"10.33483/jfpau.1330731","DOIUrl":"https://doi.org/10.33483/jfpau.1330731","url":null,"abstract":"Objective: The knowledge and attitude of pharmacists play an essential role in the patient counseling services of pharmacists. Celiac disease is one of the diseases in which patient follow-up and counseling are essential, and the number of studies dealing with the roles of pharmacists in this disease is very limited. This study aims to fill this gap and contribute to public health by evaluating community pharmacists' knowledge, attitudes, and practices for celiac disease. \u0000Material and Method: Based on the literature, a measurement tool including the knowledge, attitudes, and practices of community pharmacists for celiac disease has been developed. The measurement tool was applied online to community pharmacists in Türkiye in 2021. The obtained data were subjected to explanatory factor analysis (EFA). \u0000Result and Discussion: The number of pharmacists participating in this study is 408. A four-factor structure was obtained: knowledge of celiac disease, attitude towards celiac disease, counseling practices for celiac patients, and professional development practices. The Cronbach's alpha values of the factors were calculated between 0.794 and 0.935, which shows high reliability. These factors explained 70.343% of the total variance. The community pharmacists had positive attitudes toward counseling for celiac disease. Still, there were some deficiencies in terms of knowledge and practice. It is thought that the knowledge and awareness of pharmacists on celiac diseases can be increased by including issues related to celiac disease in both undergraduate education and vocational training programs.","PeriodicalId":7891,"journal":{"name":"Ankara Universitesi Eczacilik Fakultesi Dergisi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46368081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Amaç: Ergojenik destekler, sporcuların egzersiz öncesi, sırası ve sonrasında fiziksel performanslarını desteklemek veya iyileştirmek için kullandıkları maddeler, uygulamalar veya tekniklerdir. Bu araştırma kapsamında incelenen ergojenik maddeler çeşitli formülasyonlarda ve içeriklerde piyasada bulunabilmekle birlikte, son yıllarda sporcular tarafından en çok tercih edilen tipi, bir kısmı gıda olarak da kullanılan ve “daha zararsız olduğu düşünülen” doğal kaynaklı ergojenik desteklerdir. Doğal kaynaklı ergojenik destekler, doğru bitki, doğru drog, doğru ürün, doğru zaman, uygun doz ve uygun formülasyonda kullanıldığı takdirde sporcunun performansını desteklemektedir. Aksi halde sporcunun kullandığı bu destekten fayda görmesinin yanı sıra sağlığını olumsuz etkileyebilecek sonuçlar ortaya çıkabilmektedir. Her ne kadar sporcu için sportif performans öncelikli olsa da asıl önemli olanın sporcunun sağlığı olduğu literatürdeki çalışmalarda da bildirilmektedir. Sonuç ve Tartışma: Bu derlemede sporcular tarafından son yıllarda tercih edilen doğal kaynaklı ergojenik desteklerin PubMed, Science Direct, Google Akademik, BioMed Central, TÜBİTAK Ulakbim veri tabanlarında detaylı bir literatür taraması ile belirlenmesi, en sık kullanılanlarının ise olumlu ve olumsuz yanlarının güncel veriler ışığında incelenmesi amaçlanmıştır.
{"title":"SPORCULAR TARAFINDAN KULLANILAN DOĞAL KAYNAKLI ERGOJENİK DESTEKLER","authors":"Ece Bayir, Gözde ELGİN CEBE, Bintuğ Öztürk","doi":"10.33483/jfpau.1316067","DOIUrl":"https://doi.org/10.33483/jfpau.1316067","url":null,"abstract":"Amaç: Ergojenik destekler, sporcuların egzersiz öncesi, sırası ve sonrasında fiziksel performanslarını desteklemek veya iyileştirmek için kullandıkları maddeler, uygulamalar veya tekniklerdir. Bu araştırma kapsamında incelenen ergojenik maddeler çeşitli formülasyonlarda ve içeriklerde piyasada bulunabilmekle birlikte, son yıllarda sporcular tarafından en çok tercih edilen tipi, bir kısmı gıda olarak da kullanılan ve “daha zararsız olduğu düşünülen” doğal kaynaklı ergojenik desteklerdir. Doğal kaynaklı ergojenik destekler, doğru bitki, doğru drog, doğru ürün, doğru zaman, uygun doz ve uygun formülasyonda kullanıldığı takdirde sporcunun performansını desteklemektedir. Aksi halde sporcunun kullandığı bu destekten fayda görmesinin yanı sıra sağlığını olumsuz etkileyebilecek sonuçlar ortaya çıkabilmektedir. Her ne kadar sporcu için sportif performans öncelikli olsa da asıl önemli olanın sporcunun sağlığı olduğu literatürdeki çalışmalarda da bildirilmektedir.\u0000Sonuç ve Tartışma: Bu derlemede sporcular tarafından son yıllarda tercih edilen doğal kaynaklı ergojenik desteklerin PubMed, Science Direct, Google Akademik, BioMed Central, TÜBİTAK Ulakbim veri tabanlarında detaylı bir literatür taraması ile belirlenmesi, en sık kullanılanlarının ise olumlu ve olumsuz yanlarının güncel veriler ışığında incelenmesi amaçlanmıştır.","PeriodicalId":7891,"journal":{"name":"Ankara Universitesi Eczacilik Fakultesi Dergisi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44299971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Bektay, Beyza Tütüncü, Damla Sosyal, Muhammed Atak, F. Izzettin
Objective: Mental illnesses constitute a significant contributor to the overall burden of illness, affecting a substantial number of individuals worldwide. The aim of the study is to to measure anxiety levels, along with a questionnaire to assess the pharmacist possible role in anxiety. Material and Method: A prospective study with onlineonline questionnaire held between November 2020 and February 2021. The questionnaire was sent to the general public. The questionnaire consists of 2 sections, demographics and anxiety levels. Anxiety levels were determined by the Generalized Anxiety Disorder (GAD-7) scale. Assessment of internal consistency, reliability, binary logistic regression and correlation analysis, group comparisons were conducted utilizing the Mann-Whitney U-test. Result and Discussion: A total of 398 responses were gathered. The majority of the participant were female (256, 64.3%) and the mean number of age was 29.7±10.7. The mean GAD-7 score was 6.31±4.21. The interclass correlation coffecient of the questionnaire was 0.671 (95% CI: 0.503-0.794, F: 4.55, p<0.001) and the Cronbach’s alpha test was found as 0.854. Feeling anxious or stressed lately (OR: 2.358, %95 CI:1.321-4.210,p<0.005) and the level of education (OR:5.618, %95 CI 1.332-23.698 p<0.05) were statistically significant factors for anxiety. The importance of involving pharmacists in anxiety disorder screenings were pointed out by this study. Pharmacists' screenings effectively identify anxiety, and aids referrals to appropriate healthcare providers.
{"title":"PHARMACIST-LED ASSESSMENT OF ANXIETY LEVELS AND ASSOCIATED FACTORS: A PROSPECTIVE OBSERVATIONAL SURVEY","authors":"M. Bektay, Beyza Tütüncü, Damla Sosyal, Muhammed Atak, F. Izzettin","doi":"10.33483/jfpau.1341561","DOIUrl":"https://doi.org/10.33483/jfpau.1341561","url":null,"abstract":"Objective: Mental illnesses constitute a significant contributor to the overall burden of illness, affecting a substantial number of individuals worldwide. The aim of the study is to to measure anxiety levels, along with a questionnaire to assess the pharmacist possible role in anxiety.\u0000Material and Method: A prospective study with onlineonline questionnaire held between November 2020 and February 2021. The questionnaire was sent to the general public. The questionnaire consists of 2 sections, demographics and anxiety levels. Anxiety levels were determined by the Generalized Anxiety Disorder (GAD-7) scale. Assessment of internal consistency, reliability, binary logistic regression and correlation analysis, group comparisons were conducted utilizing the Mann-Whitney U-test. \u0000Result and Discussion: A total of 398 responses were gathered. The majority of the participant were female (256, 64.3%) and the mean number of age was 29.7±10.7. The mean GAD-7 score was 6.31±4.21. The interclass correlation coffecient of the questionnaire was 0.671 (95% CI: 0.503-0.794, F: 4.55, p<0.001) and the Cronbach’s alpha test was found as 0.854. Feeling anxious or stressed lately (OR: 2.358, %95 CI:1.321-4.210,p<0.005) and the level of education (OR:5.618, %95 CI 1.332-23.698 p<0.05) were statistically significant factors for anxiety. The importance of involving pharmacists in anxiety disorder screenings were pointed out by this study. Pharmacists' screenings effectively identify anxiety, and aids referrals to appropriate healthcare providers.","PeriodicalId":7891,"journal":{"name":"Ankara Universitesi Eczacilik Fakultesi Dergisi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47037080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: One of the major challenges in developing curcumin as a pharmaceutical agent is its low bioavailability after oral administration. Co-administration of curcumin-piperine combined with employing solid dispersions (SD) approach has been shown to enhance curcumin dissolution and bioavailability. Understanding the influence of the processing temperature during spray drying is crucial in SDs preparations; the purpose of this study is to inquire the effect of inlet temperature spray-dryer on dissolution behavior and the best-fit kinetic model of dissolution itself. Material and Method: The SD powder was prepared using a spray-drying method by varying the inlet temperature (105°C; 115°C; 125°C) and involved polyvinyl alcohol (PVA) as a carrier. The SD were prepared at 30% Curcuma longa and 10% Piper nigrum extracts. Yield (%) of the dried powder resulted from the spray drying process was monitored, and dissolution behavior of curcumin and piperine were analyzed using a dissolution efficiency (DE) value. Furthermore, mathematical model describing the release mechanism of curcumin and piperine from the dissolution were evaluated using a DDSolver software. Result and Discussion: The variation of drying temperature on the spray dryer affects the dissolution behavior and the % yield of the PVA-based SD containing C. longa and P. nigrum extract. The most ideal mathematical model of kinetic release for curcumin and piperine were the Quadratic model, indicating that the mechanism of dissolution is diffusion through a gap between the PVA particle and the surrounding medium.
{"title":"KURUTMA SICAKLIĞININ KURKUMİN VE PİPERİN ÇÖZÜNMESİNE VE KATI DİSPERSİYON BAZLI MİKROPARTİKÜLLERİN SALINIM KİNETİĞİNE ETKİLERİ: BİR ÖN ÇALIŞMA","authors":"Monica Octaviani Tiara Dewi̇, Dewi Setyani̇ngsi̇h","doi":"10.33483/jfpau.1253561","DOIUrl":"https://doi.org/10.33483/jfpau.1253561","url":null,"abstract":"Objective: One of the major challenges in developing curcumin as a pharmaceutical agent is its low bioavailability after oral administration. Co-administration of curcumin-piperine combined with employing solid dispersions (SD) approach has been shown to enhance curcumin dissolution and bioavailability. Understanding the influence of the processing temperature during spray drying is crucial in SDs preparations; the purpose of this study is to inquire the effect of inlet temperature spray-dryer on dissolution behavior and the best-fit kinetic model of dissolution itself.\u0000Material and Method: The SD powder was prepared using a spray-drying method by varying the inlet temperature (105°C; 115°C; 125°C) and involved polyvinyl alcohol (PVA) as a carrier. The SD were prepared at 30% Curcuma longa and 10% Piper nigrum extracts. Yield (%) of the dried powder resulted from the spray drying process was monitored, and dissolution behavior of curcumin and piperine were analyzed using a dissolution efficiency (DE) value. Furthermore, mathematical model describing the release mechanism of curcumin and piperine from the dissolution were evaluated using a DDSolver software.\u0000Result and Discussion: The variation of drying temperature on the spray dryer affects the dissolution behavior and the % yield of the PVA-based SD containing C. longa and P. nigrum extract. The most ideal mathematical model of kinetic release for curcumin and piperine were the Quadratic model, indicating that the mechanism of dissolution is diffusion through a gap between the PVA particle and the surrounding medium.","PeriodicalId":7891,"journal":{"name":"Ankara Universitesi Eczacilik Fakultesi Dergisi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49411285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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