Objective: The simultaneous determination of abacavir (ABV), lamivudine (LMV) and zidovudine (ZDV) were applied by dual amplitude difference method coupled with ratio difference spectrophotometric methods. Material and Method: The LMV was quantified by selected the 226.0 nm and 235.0 nm in the dual amplitude difference method. For ratio difference method 297.0 nm and 268.0 nm wavelengths and 266.0 nm, 245 nm wavelengths were chosen to quantify respectively ABV and ZDV. Accuracy studies have been carried out with percent recovery. Result and Discussion: The proposed study, three active substances used in Human immunodeficiency virus (HIV) treatment were quantified. These active ingredients are used in combination to provide effective treatment. With the applied methods, firstly LMV was determined by dual amplitut difference method, then ABV and ZDV were determined by ratio difference. The three active ingredients were studied in the concentration range of 3-21 µg/ml. Correlation coefficients were found to be between 0.9985 and 0.9996. Recovery results range from 95.2 to 106.2. In the method, it was only dissolved in the solvent and measured, and the analysis was carried out without pre-preparation and expensive equipment.
{"title":"ABAKAVİR, LAMİVUDİN VE ZİDOVUDİN’İN ÜÇLÜ KARIŞIMDA SPEKTROFOTOMETRİK ANALİZİ","authors":"Gizem Tiris, Nevin Erk","doi":"10.33483/jfpau.1329821","DOIUrl":"https://doi.org/10.33483/jfpau.1329821","url":null,"abstract":"Objective: The simultaneous determination of abacavir (ABV), lamivudine (LMV) and zidovudine (ZDV) were applied by dual amplitude difference method coupled with ratio difference spectrophotometric methods.\u0000Material and Method: The LMV was quantified by selected the 226.0 nm and 235.0 nm in the dual amplitude difference method. For ratio difference method 297.0 nm and 268.0 nm wavelengths and 266.0 nm, 245 nm wavelengths were chosen to quantify respectively ABV and ZDV. Accuracy studies have been carried out with percent recovery.\u0000Result and Discussion: The proposed study, three active substances used in Human immunodeficiency virus (HIV) treatment were quantified. These active ingredients are used in combination to provide effective treatment. With the applied methods, firstly LMV was determined by dual amplitut difference method, then ABV and ZDV were determined by ratio difference. The three active ingredients were studied in the concentration range of 3-21 µg/ml. Correlation coefficients were found to be between 0.9985 and 0.9996. Recovery results range from 95.2 to 106.2. In the method, it was only dissolved in the solvent and measured, and the analysis was carried out without pre-preparation and expensive equipment.","PeriodicalId":7891,"journal":{"name":"Ankara Universitesi Eczacilik Fakultesi Dergisi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42946776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Amaç: Bu çalışmanın amacı, farklı nesil florokinolonlardan; siprofloksasin, levofloksasin, enrofloksasin ve moksifloksasinin ayrılması ve eş zamanlı analizine olanak sağlayan yeni, kolay, hızlı ve hassas bir HPLC-DAD yöntemi geliştirmektir. Gereç ve Yöntem: Literatürlerde, etken maddede florokinolonların tek başına veya ikili karışımlarının ayrılmaları, analizi ve miktar tayinleri ile ilgili çeşitli yöntemler mevcuttur. Bu çalışmada siprofloksasin, levofloksasin, enrofloksasin ve moksifloksasin için en etkili ayırımı sağlayacak yeni bir HPLC-DAD yönteminin oluşturulması hedeflenmiştir. Farklı asidik ve bazik hareketli fazlar, tampon çözeltiler ve ayırım tipleri denenmiştir. En etkili ve seçici yöntemin XTerra, C18 (100 x 4.6 mm, tanecik boyutu 3.5 µm) analitik kolon ve metanol:borat tamponu (pH=9.1, 100 mM) içeren hareketli faz ile gradient elüsyonla 0.6 ml/dak akış hızında gerçekleştirilmiştir. Genel olarak florokinolonların kromatografik tekniklerle analizinde floresan dedektör kullanıldığı gözlenmiştir. Yaptığımız çalışmada ise ayırım 280 nm'de DAD dedektörü kullanılarak başarılmış ve siprofloksasin, levofloksasin, enrofloksasin ve moksifloksasinin eş zamanlı tayinleri gerçekleştirilmiştir. Kalibrasyon eğrileri çalışılan florokinolonların herbiri için 0.5-10 µg/ml konsantrasyon aralığında doğrusaldır. Geliştirilen yöntem için validasyon çalışmaları da yapılmıştır. Sonuç ve Tartışma: Siprofloksasin, levofloksasin, enrofloksasin ve moksifloksasinin eş zamanlı tayinine izin veren basit, hızlı, hassas ve valide bir HPLC-DAD yöntemi geliştirilmiştir.
目标：这项工作的目的是创造各种各样的氟洛金酮；一种新的、简便、快速、灵敏的HPLC-DAD方法是建立左氧氟沙星、恩诺沙星和莫西沙星的分离和同时分析方法。需要和方法是区分单独或两种混合物的不同方法、分析和定量模式。在这项研究中，一种新的HPLC-DAD方法旨在最有效地区分西洛沙星、左氟沙星、恩罗沙星和莫西沙星。测试了不同的酸性和一些运动，解决了卫生棉条和分离类型。En etkili ve seçici yöntemin XTerra，C18（100 x 4.6 mm，tanecik boyutu 3.5µm）分析甲醇：硼填充物（pH=9.1，100 mm）içeren hareketli faz ile梯度elüsyonla 0.6 ml/dak akışhızında gerçekleştirimiştir。通常情况下，氟洛沙检测器用于分析氟洛沙的色谱技术。在我们所做的工作中，相同类型的DNA检测器成功地用于280nm，并同时进行了环丙沙星、左氧氟沙星、恩诺沙星和莫西沙星的检测。对于每一条活性校准曲线，氟洛西隆的浓度范围在0.5-10µg/ml之间是正确的。已经开发了一种简单、快速、灵敏和可控的HPLC-DAD方法，以便对改进的方法进行验证。
{"title":"FLOROKİNOLONLARIN HPLC-DAD İLE ANALİZİ İÇİN YENİ YÖNTEM GELİŞTİRİLMESİ","authors":"Aysun Di̇nçel, Elif Damla GÖK TOPAK, Feyyaz Onur","doi":"10.33483/jfpau.1337313","DOIUrl":"https://doi.org/10.33483/jfpau.1337313","url":null,"abstract":"Amaç: Bu çalışmanın amacı, farklı nesil florokinolonlardan; siprofloksasin, levofloksasin, enrofloksasin ve moksifloksasinin ayrılması ve eş zamanlı analizine olanak sağlayan yeni, kolay, hızlı ve hassas bir HPLC-DAD yöntemi geliştirmektir.\u0000Gereç ve Yöntem: Literatürlerde, etken maddede florokinolonların tek başına veya ikili karışımlarının ayrılmaları, analizi ve miktar tayinleri ile ilgili çeşitli yöntemler mevcuttur. Bu çalışmada siprofloksasin, levofloksasin, enrofloksasin ve moksifloksasin için en etkili ayırımı sağlayacak yeni bir HPLC-DAD yönteminin oluşturulması hedeflenmiştir. Farklı asidik ve bazik hareketli fazlar, tampon çözeltiler ve ayırım tipleri denenmiştir. En etkili ve seçici yöntemin XTerra, C18 (100 x 4.6 mm, tanecik boyutu 3.5 µm) analitik kolon ve metanol:borat tamponu (pH=9.1, 100 mM) içeren hareketli faz ile gradient elüsyonla 0.6 ml/dak akış hızında gerçekleştirilmiştir. Genel olarak florokinolonların kromatografik tekniklerle analizinde floresan dedektör kullanıldığı gözlenmiştir. Yaptığımız çalışmada ise ayırım 280 nm'de DAD dedektörü kullanılarak başarılmış ve siprofloksasin, levofloksasin, enrofloksasin ve moksifloksasinin eş zamanlı tayinleri gerçekleştirilmiştir. Kalibrasyon eğrileri çalışılan florokinolonların herbiri için 0.5-10 µg/ml konsantrasyon aralığında doğrusaldır. Geliştirilen yöntem için validasyon çalışmaları da yapılmıştır.\u0000Sonuç ve Tartışma: Siprofloksasin, levofloksasin, enrofloksasin ve moksifloksasinin eş zamanlı tayinine izin veren basit, hızlı, hassas ve valide bir HPLC-DAD yöntemi geliştirilmiştir.","PeriodicalId":7891,"journal":{"name":"Ankara Universitesi Eczacilik Fakultesi Dergisi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49578915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: The purpose of the work was to investigate new synthetic compounds of imidazolinone-based sulfonamide derivatives as potent and selective enyzme inhibitors. A number of compounds synthesized and their inhibitory action against acetylcholine esterase (AChE), and human (h) carbonic anhydrase (CA) isoforms I and II were investigated. Material and Method: The identity of the compounds has been confirmed by HRMS, 1H NMR, and 13C NMR. The pharmacological potential of the compounds has been determined by in vitro enzyme-based assays. Result and Discussion: In this study, a series of imidazolinone-based sulfonamide derivatives were synthesized from 4-(2,4-dimethoxybenzylidene)-2-phenyloxazol-5(4H)-one, sodium acetate, glacial acetic acid, and suitable sulfonamide derivatives such as sulfaguanidine (3), sulfanilamide (4), sulfadiazine (5). These compounds showed potent inhibitory action against acetylcholine esterase (AChE), and human (h) carbonic anhydrase (CA) isoforms I and II. Compound 4 (Ki=19.53±1.23 nM) was a potent and selective inhibitor against hCA I while compound 3 (Ki=16.49±2.20 nM) was found to be potent inhibitor against hCA II. Compound 5 with Ki of 11.68±1.45 nM showed a potent inhibitory effect against the AChE enzyme. Imidazolinone-based sulfonamides can be used in the design of selective CAs inhibitors and anti-Alzheimer's compounds for further studies.
{"title":"İMİDAZOLİNON BAZLI SÜLFONAMİD TÜREVLERİ: SENTEZ, KARAKTERİZASYON VE BAZI METABOLİK ENZİMLERE KARŞI İNHİBİTÖR ÖZELLİKLERİ","authors":"Mehtap TUĞRAK SAKARYA, Halise İnci Gül, Cemil Yamali, Yeliz Demi̇r, İlhami Gülçi̇n","doi":"10.33483/jfpau.1311157","DOIUrl":"https://doi.org/10.33483/jfpau.1311157","url":null,"abstract":"Objective: The purpose of the work was to investigate new synthetic compounds of imidazolinone-based sulfonamide derivatives as potent and selective enyzme inhibitors. A number of compounds synthesized and their inhibitory action against acetylcholine esterase (AChE), and human (h) carbonic anhydrase (CA) isoforms I and II were investigated.\u0000Material and Method: The identity of the compounds has been confirmed by HRMS, 1H NMR, and 13C NMR. The pharmacological potential of the compounds has been determined by in vitro enzyme-based assays. \u0000Result and Discussion: In this study, a series of imidazolinone-based sulfonamide derivatives were synthesized from 4-(2,4-dimethoxybenzylidene)-2-phenyloxazol-5(4H)-one, sodium acetate, glacial acetic acid, and suitable sulfonamide derivatives such as sulfaguanidine (3), sulfanilamide (4), sulfadiazine (5). These compounds showed potent inhibitory action against acetylcholine esterase (AChE), and human (h) carbonic anhydrase (CA) isoforms I and II. Compound 4 (Ki=19.53±1.23 nM) was a potent and selective inhibitor against hCA I while compound 3 (Ki=16.49±2.20 nM) was found to be potent inhibitor against hCA II. Compound 5 with Ki of 11.68±1.45 nM showed a potent inhibitory effect against the AChE enzyme. Imidazolinone-based sulfonamides can be used in the design of selective CAs inhibitors and anti-Alzheimer's compounds for further studies.","PeriodicalId":7891,"journal":{"name":"Ankara Universitesi Eczacilik Fakultesi Dergisi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49190215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: Fungal infections which are relatively common mainly invades the body of an immunosuppressed patients and people undergoing therapy. These pathogens act through different pathways like the Dihydrofolate reductase (DHFR) has a role in the folate synthetic pathway which is responsible for DNA synthesis. Since the early ages herbal remedies were used and have been tested for treating these fungal infections. Previous studies have revealed the use of bioactive molecules of pteridophytes to demonstrate antifungal activity. Material and Method: In the present study different pteridophytes were selected from available library which showed the presence of bioactive phytoconstituents. In-silico studies on DHFR target (PDB ID: 6DRS and PDB ID: 3QLW) was carried out using PyRx program (India) to determine the affinity of bioactive molecules against the fungal strain. Result and Discussion: Molecular docking was performed with 11 bioactive molecules showing activity against the selected target proteins. So, we can conclude that the selected bioactive molecules are active against fungal strain and can be further investigated for both in-vivo and in-vitro studies.
{"title":"INTERACTION OF PTERIDOPHYTIC BIOACTIVE COMPOUNDS WITH FUNGAL DIHYDROFOLATE REDUCTASE ENZYME AS INHIBITOR","authors":"Manohardeep Singh, Mansi Raghav, Akanksha Si̇ngh, Akanksha Kumari̇, Prıya Bansal, S. Prakash, Abhishek Kumar","doi":"10.33483/jfpau.1270767","DOIUrl":"https://doi.org/10.33483/jfpau.1270767","url":null,"abstract":"Objective: Fungal infections which are relatively common mainly invades the body of an immunosuppressed patients and people undergoing therapy. These pathogens act through different pathways like the Dihydrofolate reductase (DHFR) has a role in the folate synthetic pathway which is responsible for DNA synthesis. Since the early ages herbal remedies were used and have been tested for treating these fungal infections. Previous studies have revealed the use of bioactive molecules of pteridophytes to demonstrate antifungal activity. \u0000Material and Method: In the present study different pteridophytes were selected from available library which showed the presence of bioactive phytoconstituents. In-silico studies on DHFR target (PDB ID: 6DRS and PDB ID: 3QLW) was carried out using PyRx program (India) to determine the affinity of bioactive molecules against the fungal strain. \u0000Result and Discussion: Molecular docking was performed with 11 bioactive molecules showing activity against the selected target proteins. So, we can conclude that the selected bioactive molecules are active against fungal strain and can be further investigated for both in-vivo and in-vitro studies.","PeriodicalId":7891,"journal":{"name":"Ankara Universitesi Eczacilik Fakultesi Dergisi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45288112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Amaç: Akciğer adenokarsinomu ve meme kanseri en sık görülen kanser türleridir ve dünya çapında kansere bağlı ölümlerin önde gelen nedenlerindendir. Safranal bileşiğinin çeşitli farmakolojik etkileri ve sitotoksik özellikleri vardır. Bu çalışmanın amacı, safranal bileşiğinin insan akciğer karsinomu hücre hattı (A549), insan meme kanseri hücre hattı (MCF-7) ve insan bronşiyal epitel sağlıklı hücre (Beas-2b) hatları üzerindeki sitotoksisitesini sürekli izleme yoluyla tahlil etmektir. Gereç ve Yöntem: Hücreler, safranal ile 1, 10, 100 µM konsantrasyonda muamele edildi ve bu bileşiğin hücre canlılığı üzerindeki etkisini belirlemek için xCELLigence gerçek zamanlı hücre analizörü kullanılmıştır. E-plaka kuyularının empedansı aracılığıyla hücre indeksi görüntülenerek her 15 dakikada bir izlenmiştir. Sonuç ve Tartışma: Yapılan çalışmalar sonucunda safranal bileşiğinin MCF-7 hücre hattı üzerinde sitotoksik etkiye sahip olduğu, A549 ve Beas-2b hücreleri üzerinde toksik etkisinin olmadığı belirlenmiştir. MCF-7 hücre hattında, hücre indeksi değişiklikleri, kontrol grubu ile karşılaştırıldığında tüm konsantrasyonlarda azaldığı tespit edilmiştir. Kanser türüne göre bu ilaçların etkinliğini artırmak için geleneksel antikanser ilaçlar ve safranal bileşiği kombinasyonu kullanılabilir.
{"title":"AKCİĞER KANSERİ VE MEME KANSERİ HÜCRELERİNDE SAFRANAL BİLEŞİĞİNİN SİTOTOKSİK AKTİVİTESİNİN GERÇEK ZAMANLI İZLENMESİ","authors":"Ebru Uzunhi̇sarcikli","doi":"10.33483/jfpau.1291975","DOIUrl":"https://doi.org/10.33483/jfpau.1291975","url":null,"abstract":"Amaç: Akciğer adenokarsinomu ve meme kanseri en sık görülen kanser türleridir ve dünya çapında kansere bağlı ölümlerin önde gelen nedenlerindendir. Safranal bileşiğinin çeşitli farmakolojik etkileri ve sitotoksik özellikleri vardır. Bu çalışmanın amacı, safranal bileşiğinin insan akciğer karsinomu hücre hattı (A549), insan meme kanseri hücre hattı (MCF-7) ve insan bronşiyal epitel sağlıklı hücre (Beas-2b) hatları üzerindeki sitotoksisitesini sürekli izleme yoluyla tahlil etmektir.\u0000Gereç ve Yöntem: Hücreler, safranal ile 1, 10, 100 µM konsantrasyonda muamele edildi ve bu bileşiğin hücre canlılığı üzerindeki etkisini belirlemek için xCELLigence gerçek zamanlı hücre analizörü kullanılmıştır. E-plaka kuyularının empedansı aracılığıyla hücre indeksi görüntülenerek her 15 dakikada bir izlenmiştir.\u0000Sonuç ve Tartışma: Yapılan çalışmalar sonucunda safranal bileşiğinin MCF-7 hücre hattı üzerinde sitotoksik etkiye sahip olduğu, A549 ve Beas-2b hücreleri üzerinde toksik etkisinin olmadığı belirlenmiştir. MCF-7 hücre hattında, hücre indeksi değişiklikleri, kontrol grubu ile karşılaştırıldığında tüm konsantrasyonlarda azaldığı tespit edilmiştir. Kanser türüne göre bu ilaçların etkinliğini artırmak için geleneksel antikanser ilaçlar ve safranal bileşiği kombinasyonu kullanılabilir.","PeriodicalId":7891,"journal":{"name":"Ankara Universitesi Eczacilik Fakultesi Dergisi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43771101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: Different types of pediatric diseases negatively affect the lives of many people, physically. Here, we aimed to document some medicinal plants used as traditional folk medicine in pediatrics treatment. Result and Discussion: 117 taxa and 53 families have been identified as traditional herbal medicines used in defined pediatric diseases. The most frequently used medicinal plant species according to the number of citations Foeniculum vulgare Mill., Juglans regia L., Dryopteris filix-mas (L.) Schott, Rosa canina L., Mentha x piperita L., Matricaria chamomilla L. All findings are expected to form the basis for new pharmaceutical products and become a handbook for healthcare professionals.
{"title":"ÇOCUK HASTALIKLARINDA GELENEKSEL OLARAK KULLANILAN BAZI BİTKİLER","authors":"Fatma Sari, Ş. Kültür, Mine Koçyi̇ği̇t","doi":"10.33483/jfpau.1214245","DOIUrl":"https://doi.org/10.33483/jfpau.1214245","url":null,"abstract":"Objective: Different types of pediatric diseases negatively affect the lives of many people, physically. Here, we aimed to document some medicinal plants used as traditional folk medicine in pediatrics treatment.\u0000Result and Discussion: 117 taxa and 53 families have been identified as traditional herbal medicines used in defined pediatric diseases. The most frequently used medicinal plant species according to the number of citations Foeniculum vulgare Mill., Juglans regia L., Dryopteris filix-mas (L.) Schott, Rosa canina L., Mentha x piperita L., Matricaria chamomilla L. All findings are expected to form the basis for new pharmaceutical products and become a handbook for healthcare professionals.","PeriodicalId":7891,"journal":{"name":"Ankara Universitesi Eczacilik Fakultesi Dergisi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47243428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: Sjögren’s syndrome is a complex and widespread autoimmune disease whose pathogenesis is not fully elucidated and environmental and genetic factors affect the development of the disease. In order to reveal the effect of genetic contribution, studies have been conducted on the genes previously shown to play a role in other autoimmune diseases such as systemic lupus erythromatosus. In addition, two GWAS studies were conducted to investigate the role of more genes in the disease by screening the entire genome and the relationship of previously unknown genes with SS was shown. Result and Discussion: Studies are being conducted with spontaneous and genetically modified animal models in order to better reveal the relationship between SS and genes and to reinforce the data obtained from humans. In this study, the relationship between the genes previously studied in other autoimmune diseases and the genes associated with SS in GWAS studies and the possible pathways that may contribute to the pathogenesis of the disease through related genes were investigated.
{"title":"SJÖGREN SENDROMU İLE BAZI GEN POLİMORFİZMLERİ ARASINDAKİ OLASI BAĞLANTILAR","authors":"Ülkü Terzi̇, İlker Ateş","doi":"10.33483/jfpau.1328811","DOIUrl":"https://doi.org/10.33483/jfpau.1328811","url":null,"abstract":"Objective: Sjögren’s syndrome is a complex and widespread autoimmune disease whose pathogenesis is not fully elucidated and environmental and genetic factors affect the development of the disease. In order to reveal the effect of genetic contribution, studies have been conducted on the genes previously shown to play a role in other autoimmune diseases such as systemic lupus erythromatosus. In addition, two GWAS studies were conducted to investigate the role of more genes in the disease by screening the entire genome and the relationship of previously unknown genes with SS was shown. \u0000Result and Discussion: Studies are being conducted with spontaneous and genetically modified animal models in order to better reveal the relationship between SS and genes and to reinforce the data obtained from humans. In this study, the relationship between the genes previously studied in other autoimmune diseases and the genes associated with SS in GWAS studies and the possible pathways that may contribute to the pathogenesis of the disease through related genes were investigated.","PeriodicalId":7891,"journal":{"name":"Ankara Universitesi Eczacilik Fakultesi Dergisi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49167537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Amaç: Hemostaz, kanamayı önlemek veya durdurmak için doğal bir işlev ve doğal bir süreçtir. Günümüzde kanama kontrolü için yeni, ekonomik ve yüksek performanslı ürünler geliştirmek için büyük çaba sarf edilmektedir. Bu çalışmada, halk hekimliğinde farklı amaçlarla kullanılan Salvia verticillata, Achillea biebersteinii, Tragopogon aureus ve Cephalaria procera gibi dört farklı bitki türünün in vitro hemostatik etkilerini değerlendirmeyi amaçladık. Gereç ve Yöntem: Farklı polaritelerde ekstreler hazırlandı ve hemostatik etkinlikleri optik agregometri kullanılarak belirlendi. Sonuç ve Tartışma: Mevcut sonuçlar, diğer bitki özleri ile karşılaştırıldığında S. verticillata ekstrelerinin adenozin-difosfat (ADP) (%80.77), kollajen (%80.78) ve araşidonik asit (AA) (%73.71) varlığında trombosit agregasyonu üzerinde en yüksek etkinliği gösterdiğini açıkça ortaya koymuştur. Yine epinefrin (EPI) varlığında en etkili trombosit agregasyonu (%47.27) C. procera uygulamasından sonra belirlendi. Ayrıca, öncelikle etil asetat ekstrelerinin APD, kollajen, AA ve EPI olgusunda en yüksek trombosit agregasyonu yüzdelerini göstermiştir. Sonuç olarak, bulgularımız, özellikle S. verticillata ve C. procera'nın etkili hemostatik ajanların yeni ve doğal kaynakları olabileceğini gösterdi.
{"title":"SALVIA VERTICILLATA L., ACHILLEA BIEBERSTEINII AFAN., TRAGOPOGON AUREUS BOISS. VE CEPHALARIA PROCERA FISCH. & AVÉ-LALL.’NIN HEMOSTATİK PERFORMANSLARININ İN VİTRO DEĞERLENDİRİLMESİ","authors":"Songül Karakaya, Özlem Özdemi̇r, Ümit İncekara, Hasan Türkez, Oksana Sytar, Özkan Aksakal","doi":"10.33483/jfpau.1266421","DOIUrl":"https://doi.org/10.33483/jfpau.1266421","url":null,"abstract":"Amaç: Hemostaz, kanamayı önlemek veya durdurmak için doğal bir işlev ve doğal bir süreçtir. Günümüzde kanama kontrolü için yeni, ekonomik ve yüksek performanslı ürünler geliştirmek için büyük çaba sarf edilmektedir. Bu çalışmada, halk hekimliğinde farklı amaçlarla kullanılan Salvia verticillata, Achillea biebersteinii, Tragopogon aureus ve Cephalaria procera gibi dört farklı bitki türünün in vitro hemostatik etkilerini değerlendirmeyi amaçladık.\u0000Gereç ve Yöntem: Farklı polaritelerde ekstreler hazırlandı ve hemostatik etkinlikleri optik agregometri kullanılarak belirlendi.\u0000Sonuç ve Tartışma: Mevcut sonuçlar, diğer bitki özleri ile karşılaştırıldığında S. verticillata ekstrelerinin adenozin-difosfat (ADP) (%80.77), kollajen (%80.78) ve araşidonik asit (AA) (%73.71) varlığında trombosit agregasyonu üzerinde en yüksek etkinliği gösterdiğini açıkça ortaya koymuştur. Yine epinefrin (EPI) varlığında en etkili trombosit agregasyonu (%47.27) C. procera uygulamasından sonra belirlendi. Ayrıca, öncelikle etil asetat ekstrelerinin APD, kollajen, AA ve EPI olgusunda en yüksek trombosit agregasyonu yüzdelerini göstermiştir. Sonuç olarak, bulgularımız, özellikle S. verticillata ve C. procera'nın etkili hemostatik ajanların yeni ve doğal kaynakları olabileceğini gösterdi.","PeriodicalId":7891,"journal":{"name":"Ankara Universitesi Eczacilik Fakultesi Dergisi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47730548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: In this study, it was aimed to develop a novel reverse-phase liquid chromatography method for the ultra-sensitive determination of the antihypertensive drug captopril, using paracetamol, which is the common pain killer, as the internal standard. Optimization of all experimental conditions including composition of mobile phase, flow rate, and column temperature was carried out step by step, and the method validity of the developed method was examined according to international validation guidelines. Calibration range, linearity, the limit of determination, the limit of quantification, robustness, accuracy from commercial tablet samples, and method stability were examined in detail. In addition, the greenness profile for the developed method was assessed with the Green Analytical Procedure Index and Analytical Greenness Calculator techniques, which are frequently used in the literature. Material and Method: The chromatographic method was conducted with an XBridge C18 column (25 cm x 4.6 mm ID; 5 µm) packed with fully porous silica materials. All analyses were performed isocratically with a mobile phase containing acetonitrile:5 mM, pH 7.0 ammonium acetate solution (50:50, v/v) at a flow rate of 1.5 ml min-1. The injection volume was 5 μl, and the column was kept at 25°C in a column oven. The column eluate was monitored at 220 nm. Under optimized conditions, retention times of captopril, and paracetamol were approximately 1.59, and 2.0 min, respectively. Result and Discussion: This study described a fully validated, simple, sensitive, accurate, linear, precise, and reproducible reversed-phase liquid chromatography method for the determination of captopril in tablet samples. Under optimal experimental conditions, the linear range was found in the range of 0.5-200 µg ml-1 and the correlation coefficient was greater than 0.99. Method precision was acceptable, with coefficients of variation between 0.05% and 0.61%. In addition, as a result of the recovery studies carried out on the tablet samples, the accuracy was found to be within satisfactory limits between 99.45% and 102.55%. Moreover, the greenness profile of the developed method also showed that the method is environmentally friendly.
目的：以常用止痛药扑热息痛为内标，建立高效液相色谱法超灵敏测定降压药卡托普利含量的新方法。逐步优化了所有实验条件，包括流动相组成、流速和柱温，并根据国际验证指南检查了所开发方法的方法有效性。详细检查了商业片剂样品的校准范围、线性、测定限、定量限、稳健性、准确性和方法稳定性。此外，使用文献中经常使用的绿色分析程序指数和分析绿色计算器技术对所开发方法的绿色度进行了评估。材料和方法：色谱法使用XBridge C18柱（25 cm x 4.6 mm ID；5µm）进行，该柱填充有完全多孔的二氧化硅材料。所有分析均使用含有乙腈：5mM，pH 7.0的乙酸铵溶液（50:50，v/v）的流动相以1.5ml min-1的流速等比例进行。进样体积为5μl，柱在柱烘箱中保持在25°C。在220nm处监测柱洗脱液。在优化的条件下，卡托普利和扑热息痛的保留时间分别约为1.59和2.0分钟。结果与讨论：本研究建立了一种完全有效、简便、灵敏、准确、线性、精密、重现性好的反相液相色谱法测定片剂中卡托普利的含量。在最佳实验条件下，线性范围为0.5-200µg ml-1，相关系数大于0.99。方法精密度可接受，变异系数在0.05%至0.61%之间。此外，对片剂样品进行的回收率研究表明，准确度在99.45%至102.55%之间，在令人满意的范围内。此外，所开发方法的绿色度曲线也表明该方法对环境友好。
{"title":"GREEN PROCEDURE INDEX ASSESSMENT OF THE NOVEL STABILITY-INDICATING RP-HPLC METHOD FOR THE DETERMINATION OF CAPTOPRIL FROM PHARMACEUTICAL DOSAGE FORM","authors":"Cem Erkmen, B. Uslu","doi":"10.33483/jfpau.1319958","DOIUrl":"https://doi.org/10.33483/jfpau.1319958","url":null,"abstract":"Objective: In this study, it was aimed to develop a novel reverse-phase liquid chromatography method for the ultra-sensitive determination of the antihypertensive drug captopril, using paracetamol, which is the common pain killer, as the internal standard. Optimization of all experimental conditions including composition of mobile phase, flow rate, and column temperature was carried out step by step, and the method validity of the developed method was examined according to international validation guidelines. Calibration range, linearity, the limit of determination, the limit of quantification, robustness, accuracy from commercial tablet samples, and method stability were examined in detail. In addition, the greenness profile for the developed method was assessed with the Green Analytical Procedure Index and Analytical Greenness Calculator techniques, which are frequently used in the literature.\u0000Material and Method: The chromatographic method was conducted with an XBridge C18 column (25 cm x 4.6 mm ID; 5 µm) packed with fully porous silica materials. All analyses were performed isocratically with a mobile phase containing acetonitrile:5 mM, pH 7.0 ammonium acetate solution (50:50, v/v) at a flow rate of 1.5 ml min-1. The injection volume was 5 μl, and the column was kept at 25°C in a column oven. The column eluate was monitored at 220 nm. Under optimized conditions, retention times of captopril, and paracetamol were approximately 1.59, and 2.0 min, respectively.\u0000Result and Discussion: This study described a fully validated, simple, sensitive, accurate, linear, precise, and reproducible reversed-phase liquid chromatography method for the determination of captopril in tablet samples. Under optimal experimental conditions, the linear range was found in the range of 0.5-200 µg ml-1 and the correlation coefficient was greater than 0.99. Method precision was acceptable, with coefficients of variation between 0.05% and 0.61%. In addition, as a result of the recovery studies carried out on the tablet samples, the accuracy was found to be within satisfactory limits between 99.45% and 102.55%. Moreover, the greenness profile of the developed method also showed that the method is environmentally friendly.","PeriodicalId":7891,"journal":{"name":"Ankara Universitesi Eczacilik Fakultesi Dergisi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49424338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: Vaccination plays a crucial role in the protection against the Covid 19 infection. However, pregnant and lactating women are excluded from clinical trials of vaccines due to the unknown effects of the vaccine on the expectant mother, fetus, and infant. Pregnancy and the following lactation periods are long processes with unique physiological, psychological, and pathological characteristics, in which many practices are discussed for the mother and the baby. Based on the limited data available on the mechanisms of action of vaccine types, Covid 19 vaccines cannot possibly cause any risk to pregnant women and nursing mothers. On the other hand, there is currently insufficient data on the safety of Covid 19 vaccines in pregnant and lactating women. Result and Discussion: Evidence-based and personalized information about vaccines is needed to support pregnant and breastfeeding women's decision-making about vaccines. Vaccination should be recommended to all pregnant and lactating women after they have been sufficiently informed about the advantage and risks of Covid 19 vaccines and their consent has been obtained. This brief review was conducted to discuss vaccination against Covid 19 infection during pregnancy and the lactation period based on scientific data and literature.
{"title":"VACCINATION AGAINST COVID 19 INFECTION DURING PREGNANCY AND LACTATION: A BRIEF REVIEW","authors":"Asım Emre Bi̇çer, A. Pehlivanlı, A. Özçelikay","doi":"10.33483/jfpau.1261673","DOIUrl":"https://doi.org/10.33483/jfpau.1261673","url":null,"abstract":"Objective: Vaccination plays a crucial role in the protection against the Covid 19 infection. However, pregnant and lactating women are excluded from clinical trials of vaccines due to the unknown effects of the vaccine on the expectant mother, fetus, and infant. Pregnancy and the following lactation periods are long processes with unique physiological, psychological, and pathological characteristics, in which many practices are discussed for the mother and the baby. Based on the limited data available on the mechanisms of action of vaccine types, Covid 19 vaccines cannot possibly cause any risk to pregnant women and nursing mothers. On the other hand, there is currently insufficient data on the safety of Covid 19 vaccines in pregnant and lactating women. \u0000Result and Discussion: Evidence-based and personalized information about vaccines is needed to support pregnant and breastfeeding women's decision-making about vaccines. Vaccination should be recommended to all pregnant and lactating women after they have been sufficiently informed about the advantage and risks of Covid 19 vaccines and their consent has been obtained. This brief review was conducted to discuss vaccination against Covid 19 infection during pregnancy and the lactation period based on scientific data and literature.","PeriodicalId":7891,"journal":{"name":"Ankara Universitesi Eczacilik Fakultesi Dergisi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45548074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：