Pub Date : 2024-10-07DOI: 10.1016/j.aqrep.2024.102404
Mina Rahbar , Roghieh Safari , Carlos I. Perez-Rostro
The Persian sturgeon (Acipenser persicus) is one of the native species of the Caspian Sea sturgeon, which holds high economic value as a source of caviar and meat. This study aimed to establish a consensus on economic traits and achieve desired genetic gains in this fish using an analytic hierarchy process (AHP) and weighted goal programming (WGP). To determine preference values for important economic traits through multiple pairwise comparisons, a survey was conducted among 45 Persian sturgeon farmers in Iran. The AHP was utilized to evaluate individual preference values using Super Decisions software. The average values of individual preferences indicated that farmers prioritize the body weight of broodstock, total caviar weight and ovarian fat lobe weight traits over others. Group preference values were determined using the WGP model in LINGO software. The results revealed that the two categories of commercial products and water temperature exhibited the most discrepancies among the groups. Extended WGP was employed to establish Consensus preference values for these categories in LINGO software. The mean of the Consensus preference values multiplied by the percentage of genetic improvement were used to calculate the desired genetic gains. The findings indicated that farmers prioritize genetic gains of total caviar weight over other traits. Our results constitute the first report on determining the importance of economic traits and multi-traits selection in the Persian sturgeon breeding program using participatory approaches.
{"title":"Defining breeding objectives and estimation economic values of traits for Persian sturgeon (Acipenser persicus)","authors":"Mina Rahbar , Roghieh Safari , Carlos I. Perez-Rostro","doi":"10.1016/j.aqrep.2024.102404","DOIUrl":"10.1016/j.aqrep.2024.102404","url":null,"abstract":"<div><div>The Persian sturgeon (<em>Acipenser persicus</em>) is one of the native species of the Caspian Sea sturgeon, which holds high economic value as a source of caviar and meat. This study aimed to establish a consensus on economic traits and achieve desired genetic gains in this fish using an analytic hierarchy process (AHP) and weighted goal programming (WGP). To determine preference values for important economic traits through multiple pairwise comparisons, a survey was conducted among 45 Persian sturgeon farmers in Iran. The AHP was utilized to evaluate individual preference values using Super Decisions software. The average values of individual preferences indicated that farmers prioritize the body weight of broodstock, total caviar weight and ovarian fat lobe weight traits over others. Group preference values were determined using the WGP model in LINGO software. The results revealed that the two categories of commercial products and water temperature exhibited the most discrepancies among the groups. Extended WGP was employed to establish Consensus preference values for these categories in LINGO software. The mean of the Consensus preference values multiplied by the percentage of genetic improvement were used to calculate the desired genetic gains. The findings indicated that farmers prioritize genetic gains of total caviar weight over other traits. Our results constitute the first report on determining the importance of economic traits and multi-traits selection in the Persian sturgeon breeding program using participatory approaches.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102404"},"PeriodicalIF":3.2,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142428843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-05DOI: 10.1016/j.aqrep.2024.102399
Hemat A. Abd El-Salam , Gamal A. Abdel-Rahman , Hemat K. Mahmoud , Tamer Ahmed Ismail , Mahmoud Alagawany
The present research amid to investigate the toxic effect of diazinon (DZN) and assess the modulatory role of chitosan nanoparticle (ChNP), orange peel extract (OPE), spirulina platensis (SP) and curcumin nanoparticles (CNP) as feed additives in O. niloticus fish. A total of 900 fish were divided into six equal groups, each with 3 replicates by random distribution. The 1st group was kept as the control group which was fed a basal diet. The 2nd group (DZN) was given a basic diet and exposed to 1/10th of the 96-hour LC50 of DZN. The 3rd group (DZN+ChNP) was exposed to DZN (0.28 mg/L) + 1 gm ChNP/kg diet. The 4th group (DZN+OPE) was exposed to DZN (0.28 mg/L) + 2 gm OPE/kg diet. The 5th group (DZN+SP) was exposed to DZN (0.28 mg/L) + 5 gm SP/kg diet, while, the 6th group (DZN+SP) was exposed to DZN (0.28 mg/L) + 150 mg CNP/kg diet. Apart from feed conversion, DZN decreased fish's growth performance parameters as compared to the control, while these values were modulated with ChNP, SP or CNP supplement to fish with DZN exposure. Fish exposed to DZN had the lowest values Hb, RBCs WBCs, total protein, albumin and globulin and the highest values of ALT, AST and uric acid while dietary supplementation with ChNP, OPE, SP and CNP to DZN exposed fish restored their levels to the control values. Fish group fed diet supplemented with DZN+OPE or DZN+ChNP recorded the lowest value of ALT among all studied groups. DZN altered the lipid profile of exposed fish while the DZN+SP group had the best values of all lipid profile among all groups followed by DZN+CNP group. Fish exposed to DZN had lower serum lysozyme activity, immunoglobulin (M, A and G) levels and antioxidant parameters (SOD, GSH, CAT and TAC) while dietary supplementation of ChNP or OPE to DZN exposed fish had higher values for these traits but still under control values. The highest level of lipid peroxidation marker malondialdehyde (MDA) was observed in DZN group while dietary supplementation of ChNP or OPE to DZN exposed fish decreased the MDA level. According to the genetic distance and dendrogram for the analysis of genetic diversity between treatments, compared to control; it is clear that the highest genetic diversity was found with DZN group samples (4.80) while dietary treatments with ChNP, OPE or SP to DZN exposed fish showed the lowest diversity of (3.00, 3.32 and 3.87), respectively. It could be concluded that long-term exposure to a sub-lethal DZN dose resulted in growth retardation, haemato-biochemical alteration, immune depression, oxidative stress and genetic material damage of Nile tilapia. Furthermore, the antioxidant and protective properties of ChNP, OPE, SP, and CNP induced nearly total protection in fish exposed to DZN through diet.
{"title":"The role of some biological and natural feed additives against the deleterious effect of diazinon in Nile tilapia (Oreochromis niloticus)","authors":"Hemat A. Abd El-Salam , Gamal A. Abdel-Rahman , Hemat K. Mahmoud , Tamer Ahmed Ismail , Mahmoud Alagawany","doi":"10.1016/j.aqrep.2024.102399","DOIUrl":"10.1016/j.aqrep.2024.102399","url":null,"abstract":"<div><div>The present research amid to investigate the toxic effect of diazinon (DZN) and assess the modulatory role of chitosan nanoparticle (ChNP), orange peel extract (OPE), <em>spirulina platensis</em> (SP) and curcumin nanoparticles (CNP) as feed additives in <em>O. niloticus</em> fish. A total of 900 fish were divided into six equal groups, each with 3 replicates by random distribution. The 1st group was kept as the control group which was fed a basal diet. The 2nd group (DZN) was given a basic diet and exposed to 1/10th of the 96-hour LC50 of DZN. The 3rd group (DZN+ChNP) was exposed to DZN (0.28 mg/L) + 1 gm ChNP/kg diet. The 4th group (DZN+OPE) was exposed to DZN (0.28 mg/L) + 2 gm OPE/kg diet. The 5th group (DZN+SP) was exposed to DZN (0.28 mg/L) + 5 gm SP/kg diet, while, the 6th group (DZN+SP) was exposed to DZN (0.28 mg/L) + 150 mg CNP/kg diet. Apart from feed conversion, DZN decreased fish's growth performance parameters as compared to the control, while these values were modulated with ChNP, <em>SP</em> or CNP supplement to fish with DZN exposure. Fish exposed to DZN had the lowest values Hb, RBCs WBCs, total protein, albumin and globulin and the highest values of ALT, AST and uric acid while dietary supplementation with ChNP, OPE, <em>SP</em> and CNP to DZN exposed fish restored their levels to the control values. Fish group fed diet supplemented with DZN+OPE or DZN+ChNP recorded the lowest value of ALT among all studied groups. DZN altered the lipid profile of exposed fish while the DZN+SP group had the best values of all lipid profile among all groups followed by DZN+CNP group. Fish exposed to DZN had lower serum lysozyme activity, immunoglobulin (M, A and G) levels and antioxidant parameters (SOD, GSH, CAT and TAC) while dietary supplementation of ChNP or OPE to DZN exposed fish had higher values for these traits but still under control values. The highest level of lipid peroxidation marker malondialdehyde (MDA) was observed in DZN group while dietary supplementation of ChNP or OPE to DZN exposed fish decreased the MDA level. According to the genetic distance and dendrogram for the analysis of genetic diversity between treatments, compared to control; it is clear that the highest genetic diversity was found with DZN group samples (4.80) while dietary treatments with ChNP, OPE or <em>SP</em> to DZN exposed fish showed the lowest diversity of (3.00, 3.32 and 3.87), respectively. It could be concluded that long-term exposure to a sub-lethal DZN dose resulted in growth retardation, haemato-biochemical alteration, immune depression, oxidative stress and genetic material damage of Nile tilapia. Furthermore, the antioxidant and protective properties of ChNP, OPE, <em>SP</em>, and CNP induced nearly total protection in fish exposed to DZN through diet.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102399"},"PeriodicalIF":3.2,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142428844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-05DOI: 10.1016/j.aqrep.2024.102396
Chandan Debnath
This study explored the thermal tolerance and routine metabolic rate of ten small indigenous fish species from Northeast India: Amblypharyngodon mola, Esomus danrica, Puntius sophore, Gudusia chapra, Heteropneustes fossilis, Botia dario, Lepidocephalichthys guntea, Mystus cavasius, Aplocheilus panchax, and Glossogobius giuris. Fish were acclimated to 20°C, 25°C, and 30°C for two weeks prior to experiments and assessed for critical thermal maxima (CTmax), critical thermal minima (CTmin), lethal thermal maxima (LTmax), oxygen consumption rates, and respiratory quotients using standardized methods. The results revealed significant interspecific variations: CTmax ranged from 36.4°C to 41.7°C, CTmin from 8.7°C to 15.2°C, and LTmax from 41.5°C to 44.9°C. Oxygen consumption rates varied between 0.26 and 1.07 mg O₂/g/h, with respiratory quotients ranging from 0.76 to 1.01. Heteropneustes fossilis (CTmax: 41.7°C at 30°C acclimation) exhibited the highest thermal tolerance, while Amblypharyngodon mola had the lowest (CTmax: 38.2°C at 30°C acclimation). Differences in thermal tolerance between species were statistically significant (p<0.05). Notably, CTmax was positively correlated with oxygen consumption rates, suggesting a connection between metabolic rate and heat tolerance. These findings enhance our understanding of the physiological adaptations of these species to their thermal environments and underscore their conservation needs amidst climate change.
{"title":"Assessing the thermal limits and metabolic profiles of small indigenous fish species: Informing conservation and aquaculture in a changing climate","authors":"Chandan Debnath","doi":"10.1016/j.aqrep.2024.102396","DOIUrl":"10.1016/j.aqrep.2024.102396","url":null,"abstract":"<div><div>This study explored the thermal tolerance and routine metabolic rate of ten small indigenous fish species from Northeast India: <em>Amblypharyngodon mola</em>, <em>Esomus danrica</em>, <em>Puntius sophore</em>, <em>Gudusia chapra</em>, <em>Heteropneustes fossilis</em>, <em>Botia dario</em>, <em>Lepidocephalichthys guntea</em>, <em>Mystus cavasius</em>, <em>Aplocheilus panchax</em>, and <em>Glossogobius giuris</em>. Fish were acclimated to 20°C, 25°C, and 30°C for two weeks prior to experiments and assessed for critical thermal maxima (CTmax), critical thermal minima (CTmin), lethal thermal maxima (LTmax), oxygen consumption rates, and respiratory quotients using standardized methods. The results revealed significant interspecific variations: CTmax ranged from 36.4°C to 41.7°C, CTmin from 8.7°C to 15.2°C, and LTmax from 41.5°C to 44.9°C. Oxygen consumption rates varied between 0.26 and 1.07 mg O₂/g/h, with respiratory quotients ranging from 0.76 to 1.01. <em>Heteropneustes fossilis</em> (CTmax: 41.7°C at 30°C acclimation) exhibited the highest thermal tolerance, while <em>Amblypharyngodon mola</em> had the lowest (CTmax: 38.2°C at 30°C acclimation). Differences in thermal tolerance between species were statistically significant (p<0.05). Notably, CTmax was positively correlated with oxygen consumption rates, suggesting a connection between metabolic rate and heat tolerance. These findings enhance our understanding of the physiological adaptations of these species to their thermal environments and underscore their conservation needs amidst climate change.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102396"},"PeriodicalIF":3.2,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142428839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-05DOI: 10.1016/j.aqrep.2024.102401
Mingsheng Xie , Hao Liu , Weibin Huang , Menglong Zhou , Shuang Zhang , Beiping Tan , Shuyan Chi , Yuanzhi Yang , Xiaohui Dong
This research examined the impacts of varying silybin dosages in high-lipid diets on the growth, hepatic histology, immunity, and immune-related gene expression of juvenile hybrid grouper (Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂). The grouper (with an initial body weight of 8.27 ± 0.08 g) were fed diets containing silybin at levels of 0 g/kg (S1, control group), 0.05 g/kg (S2), 0.10 g/kg (S3), 0.15 g/kg (S4), 0.20 g/kg (S5), 0.25 g/kg (S6), and 0.50 g/kg (S7) for 8 weeks. The study results suggest that the silybin-treated groups displayed an initial increase followed by a decrease in final body weight and specific growth rate, with the highest value observed in the group S5. In serum samples, silybin supplementation of high-lipid diets resulted in increased activities of alkaline phosphatase (AKP), superoxide dismutase (SOD) and catalase (CAT) enzymes, decreased activities of aspartate transaminase (AST) and alanine transaminase (ALT) enzymes and increased total antioxidant capacity (T-AOC) content. In liver, the addition of silybin to high-lipid diets increased SOD, CAT, and lysozyme (LYZ) enzyme activities, increased T-AOC and immunoglobulin M (IgM) contents, and decreased reactive oxygen species (ROS) and malondialdehyde (MDA) contents. Contrasted with the control group, the addition of silybin at 0.05–0.50 g/kg enhanced the hepatic histomorphology, for example, ameliorating the indistinctness of cell outlines and diminishing the hepatocyte vacuolation. Silybin treatment significantly upregulated the expression of liver sod, cat, gpx, nrf2, keap1, hsp70, hsp90, tlr22, myd88, il-1β, tnf-α and il-6(P < 0.05). Silybin treatment significantly upregulated the expression of tlr22, myd88, il-1β, tnf-α, and il-6 of head kidney (P < 0.05). After vibrio harveyi challenge, groups S5-S6 showed higher survival than the control. Incorporating silybin into high-lipid diets boosts grouper's growth, antioxidant, and immune abilities. Regression analysis implies adding 0.23 g/kg silybin to the diets of juvenile hybrid grouper.
{"title":"Effects of silybin on growth performance, antioxidant capacity and immunity in juvenile hybrid grouper (Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂) fed with high-lipid diets","authors":"Mingsheng Xie , Hao Liu , Weibin Huang , Menglong Zhou , Shuang Zhang , Beiping Tan , Shuyan Chi , Yuanzhi Yang , Xiaohui Dong","doi":"10.1016/j.aqrep.2024.102401","DOIUrl":"10.1016/j.aqrep.2024.102401","url":null,"abstract":"<div><div>This research examined the impacts of varying silybin dosages in high-lipid diets on the growth, hepatic histology, immunity, and immune-related gene expression of juvenile hybrid grouper (<em>Epinephelus fuscoguttatus</em> ♀ × <em>Epinephelus lanceolatus</em> ♂). The grouper (with an initial body weight of 8.27 ± 0.08 g) were fed diets containing silybin at levels of 0 g/kg (S1, control group), 0.05 g/kg (S2), 0.10 g/kg (S3), 0.15 g/kg (S4), 0.20 g/kg (S5), 0.25 g/kg (S6), and 0.50 g/kg (S7) for 8 weeks. The study results suggest that the silybin-treated groups displayed an initial increase followed by a decrease in final body weight and specific growth rate, with the highest value observed in the group S5. In serum samples, silybin supplementation of high-lipid diets resulted in increased activities of alkaline phosphatase (AKP), superoxide dismutase (SOD) and catalase (CAT) enzymes, decreased activities of aspartate transaminase (AST) and alanine transaminase (ALT) enzymes and increased total antioxidant capacity (T-AOC) content. In liver, the addition of silybin to high-lipid diets increased SOD, CAT, and lysozyme (LYZ) enzyme activities, increased T-AOC and immunoglobulin M (IgM) contents, and decreased reactive oxygen species (ROS) and malondialdehyde (MDA) contents. Contrasted with the control group, the addition of silybin at 0.05–0.50 g/kg enhanced the hepatic histomorphology, for example, ameliorating the indistinctness of cell outlines and diminishing the hepatocyte vacuolation. Silybin treatment significantly upregulated the expression of liver <em>sod</em>, <em>cat</em>, <em>gpx</em>, <em>nrf2</em>, <em>keap1</em>, <em>hsp70</em>, <em>hsp90</em>, <em>tlr22</em>, <em>myd88</em>, <em>il-1β</em>, <em>tnf-α</em> and <em>il-6</em>(<em>P</em> < 0.05). Silybin treatment significantly upregulated the expression of <em>tlr22</em>, <em>myd88</em>, <em>il-1β</em>, <em>tnf-α</em>, and <em>il-6</em> of head kidney (<em>P</em> < 0.05). After <em>vibrio harveyi</em> challenge, groups S5-S6 showed higher survival than the control. Incorporating silybin into high-lipid diets boosts grouper's growth, antioxidant, and immune abilities. Regression analysis implies adding 0.23 g/kg silybin to the diets of juvenile hybrid grouper.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102401"},"PeriodicalIF":3.2,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142428845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-04DOI: 10.1016/j.aqrep.2024.102406
Ran Cui, Jie Huang, Saisai Wang, Xin Zhang
Mandarin fish is an important freshwater economic fish in China, and the genome of mandarin fish population may have specific selection signatures after long-term selection, and studying these selection signatures is one of the important strategies for screening functional genes. In this study, the whole genome was resequenced from five mandarin fish populations (HLJ, HN, AH, FJ, and BJ), and a total of high-quality SNPs with 1659,318 single nucleotide polymorphisms were obtained, and the genetic differentiation index and nucleotide polymorphism ratio between populations were used to detect the selected genomic regions. The results showed that all individuals were divided into two branches, with the HLJ population forming an independent cluster, and the HN, AH, FJ, and BJ populations clustering together. Selection signal analysis on the HLJ mandarin fish population and other mandarin fish populations using the FST and π HLJ/other methods. Finally, we identified 26 important candidate genes based on the enrichment pathways related to fat traits such as Glycerolipid metabolism, acyl-CoA oxidase activity, Wnt signaling pathway, and Notch signaling pathway, including notch3, pla2g12a and acox1. This study explores the genetic diversity and genetic structure of mandarin fish from a whole-genome perspective, identifies candidate genes related to fat traits, and provides references for the protection and utilization of mandarin fish genetic resources.
{"title":"Whole-genome resequencing reveals genetic diversity and selection signatures in five populations of Mandarin Fish (Siniperca chuatsi)","authors":"Ran Cui, Jie Huang, Saisai Wang, Xin Zhang","doi":"10.1016/j.aqrep.2024.102406","DOIUrl":"10.1016/j.aqrep.2024.102406","url":null,"abstract":"<div><div>Mandarin fish is an important freshwater economic fish in China, and the genome of mandarin fish population may have specific selection signatures after long-term selection, and studying these selection signatures is one of the important strategies for screening functional genes. In this study, the whole genome was resequenced from five mandarin fish populations (HLJ, HN, AH, FJ, and BJ), and a total of high-quality SNPs with 1659,318 single nucleotide polymorphisms were obtained, and the genetic differentiation index and nucleotide polymorphism ratio between populations were used to detect the selected genomic regions. The results showed that all individuals were divided into two branches, with the HLJ population forming an independent cluster, and the HN, AH, FJ, and BJ populations clustering together. Selection signal analysis on the HLJ mandarin fish population and other mandarin fish populations using the FST and π HLJ/other methods. Finally, we identified 26 important candidate genes based on the enrichment pathways related to fat traits such as Glycerolipid metabolism, acyl-CoA oxidase activity, Wnt signaling pathway, and Notch signaling pathway, including <em>notch3</em>, <em>pla2g12a</em> and <em>acox1</em>. This study explores the genetic diversity and genetic structure of mandarin fish from a whole-genome perspective, identifies candidate genes related to fat traits, and provides references for the protection and utilization of mandarin fish genetic resources.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102406"},"PeriodicalIF":3.2,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142428842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lipids are key nutrients that affect the development of the ovary in aquatic animals. This study aimed to investigate the effects of different lipid sources on the ovarian development and health of Macrobrachium rosenbergii broodstock. The M. rosenbergii broodstock, with an initial body weight of 8.07±0.74 g, were fed diets containing 5 % fish oil (FO), soya bean oil (SO), rapeseed oil (RO), coconut oil (CO), and a 1:1 mixture of fish oil and soybean oil (MO) as lipid sources for a period of 56 days. The results indicated that there were no significant differences in survival rate (SR) and hepatopancreatic index (HSI) among the different groups (P > 0.05). However, the specific growth rate (SGR) and gonadosomatic index (GSI) in the MO group were significantly higher than other groups (P < 0.05). Additionally, fatty acid levels in diets directly influenced the fatty acid composition of prawn hepatopancreas, showing a similar trend to that observed in the diet itself. Furthermore, compared to the other trial groups, haemolymph levels of 17-β estradiol (E2) and progesterone (PROG) were significantly increased in the MO group (P < 0.05). The ovarian section also exhibited better histomorphology and larger oocyte diameter in this group. Moreover, total antioxidant capacity was significantly increased in the MO group (P < 0.05). Saturated fatty acids in RO and CO groups could promote the expression of lipid synthesis-related genes fatty acid synthase (fas) and acetyl-CoA carboxylase (acc), concurrently suppressing the expression of lipid catabolism-related genes acyl-CoA oxidase-1 (acox-1) and AMP-activated protein kinase alpha (ampkα) (P < 0.05). Conversely, polyunsaturated fatty acids in the MO group significantly up-regulated the expression levels of fatty acid binding protein-3 (fabp-3) and peroxisome proliferator-activated receptor gamma (pparγ) (P < 0.05). Based on the findings of hepatopancreatic tissue structure, it was determined that the use of a single vegetable oil resulted in some damage to the hepatopancreatic tissue structure of M. rosenbergii broodstock, leading to increased oxidative stress. In conclusion, it is recommended that a 1:1 mixture of fish oil and soybean oil be used as a dietary lipid source to effectively meet the nutritional requirements during the ovarian development period of M. rosenbergii broodstock. This combination also enhances antioxidant properties, promotes the synthesis of haemolymph steroid hormones and lipid metabolism in female prawns, thereby facilitating ovarian development and overall health of M. rosenbergii broodstock.
{"title":"Positive effects of a mixture of fish oil and soybean oil as a dietary lipid source on the ovarian development and health of female giant river prawn, Macrobrachium rosenbergii broodstock","authors":"Yonghui Jian , Jiaxin Song , Zhiyuan Chen, Youqin Kong, Changle Qi, Zhili Ding, Qiyou Xu","doi":"10.1016/j.aqrep.2024.102407","DOIUrl":"10.1016/j.aqrep.2024.102407","url":null,"abstract":"<div><div>Lipids are key nutrients that affect the development of the ovary in aquatic animals. This study aimed to investigate the effects of different lipid sources on the ovarian development and health of <em>Macrobrachium rosenbergii</em> broodstock. The <em>M. rosenbergii</em> broodstock, with an initial body weight of 8.07±0.74 g, were fed diets containing 5 % fish oil (FO), soya bean oil (SO), rapeseed oil (RO), coconut oil (CO), and a 1:1 mixture of fish oil and soybean oil (MO) as lipid sources for a period of 56 days. The results indicated that there were no significant differences in survival rate (SR) and hepatopancreatic index (HSI) among the different groups (<em>P</em> > 0.05). However, the specific growth rate (SGR) and gonadosomatic index (GSI) in the MO group were significantly higher than other groups (<em>P</em> < 0.05). Additionally, fatty acid levels in diets directly influenced the fatty acid composition of prawn hepatopancreas, showing a similar trend to that observed in the diet itself. Furthermore, compared to the other trial groups, haemolymph levels of 17-β estradiol (E<sub>2</sub>) and progesterone (PROG) were significantly increased in the MO group (<em>P</em> < 0.05). The ovarian section also exhibited better histomorphology and larger oocyte diameter in this group. Moreover, total antioxidant capacity was significantly increased in the MO group (<em>P</em> < 0.05). Saturated fatty acids in RO and CO groups could promote the expression of lipid synthesis-related genes fatty acid synthase (<em>fas</em>) and acetyl-CoA carboxylase (<em>acc</em>), concurrently suppressing the expression of lipid catabolism-related genes acyl-CoA oxidase-1 (<em>acox-1</em>) and AMP-activated protein kinase alpha (<em>ampkα</em>) (<em>P < 0.05</em>). Conversely, polyunsaturated fatty acids in the MO group significantly up-regulated the expression levels of fatty acid binding protein-3 (<em>fabp-3</em>) and peroxisome proliferator-activated receptor gamma (<em>pparγ</em>) (<em>P < 0.05</em>). Based on the findings of hepatopancreatic tissue structure, it was determined that the use of a single vegetable oil resulted in some damage to the hepatopancreatic tissue structure of <em>M. rosenbergii</em> broodstock, leading to increased oxidative stress. In conclusion, it is recommended that a 1:1 mixture of fish oil and soybean oil be used as a dietary lipid source to effectively meet the nutritional requirements during the ovarian development period of <em>M. rosenbergii</em> broodstock. This combination also enhances antioxidant properties, promotes the synthesis of haemolymph steroid hormones and lipid metabolism in female prawns, thereby facilitating ovarian development and overall health of <em>M. rosenbergii</em> broodstock.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102407"},"PeriodicalIF":3.2,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142428102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-04DOI: 10.1016/j.aqrep.2024.102393
Kemeng Jiang , Wenqian Wang , Jianlin Li , Wenrong Feng , Ezra Martini Kamunga , Zhihua Zhang , Yongkai Tang
Salty-alkaline waters are widely distributed globally, and how to effectively develop saline-alkaline water to improve the utilization rate of water resources has become a global challenge. This study examined the survival performance of juvenile silver crucian carp (Carassius gibelio) under alkaline gradient acclimation conditions and explored the regulatory mechanisms for their adaptation to high alkalinity. Compared to directly immersing in high concentrations, alkaline gradient acclimation significantly improved the survival rate of juvenile silver crucian carp. Long-term high alkaline stress caused significant reduction in the growth performance of juvenile silver crucian carp. After alkaline stress, the enzyme activities of Na+-K+-ATP (NKA) and Ca2+-Mg2+-ATP (CMA) in the gills were significantly decreased. Meanwhile, histopathology of the gills showed structural changes, indicating that the physiological functions of juvenile silver crucian carp were impaired under alkaline conditions. Transcriptomic analysis found that the expression of genes related to metabolism and immune response pathways in the gill and kidney underwent significant changes. Genes related to carbohydrate metabolism were upregulated while those related to protein metabolism were downregulated, indicating that alkaline stress caused the organism to utilize carbohydrates rather than proteins as much as possible to meet enhanced metabolism. The HIF pathway that maintains the body's survival was significantly up-regulated, at the expense of high energy consumption. Dysregulation of genes related to the formation of neutrophil extracellular traps (NETosis) pathway indicated that the immune function of the fish was suppressed. Overall, this study revealed the physiological and molecular responses of juvenile silver crucian carp to alkaline stress, deepening our understanding of their adaptation strategies and the potential impact of alkaline environments on aquatic life.
{"title":"Physiological and molecular responses of juvenile silver crucian carp (Carassius gibelio) to long-term high alkaline stress: Growth performance, histopathology, and transcriptomic analysis","authors":"Kemeng Jiang , Wenqian Wang , Jianlin Li , Wenrong Feng , Ezra Martini Kamunga , Zhihua Zhang , Yongkai Tang","doi":"10.1016/j.aqrep.2024.102393","DOIUrl":"10.1016/j.aqrep.2024.102393","url":null,"abstract":"<div><div>Salty-alkaline waters are widely distributed globally, and how to effectively develop saline-alkaline water to improve the utilization rate of water resources has become a global challenge. This study examined the survival performance of juvenile silver crucian carp (<em>Carassius gibelio</em>) under alkaline gradient acclimation conditions and explored the regulatory mechanisms for their adaptation to high alkalinity. Compared to directly immersing in high concentrations, alkaline gradient acclimation significantly improved the survival rate of juvenile silver crucian carp. Long-term high alkaline stress caused significant reduction in the growth performance of juvenile silver crucian carp. After alkaline stress, the enzyme activities of Na<sup>+</sup>-K<sup>+</sup>-ATP (NKA) and Ca<sup>2+</sup>-Mg<sup>2+</sup>-ATP (CMA) in the gills were significantly decreased. Meanwhile, histopathology of the gills showed structural changes, indicating that the physiological functions of juvenile silver crucian carp were impaired under alkaline conditions. Transcriptomic analysis found that the expression of genes related to metabolism and immune response pathways in the gill and kidney underwent significant changes. Genes related to carbohydrate metabolism were upregulated while those related to protein metabolism were downregulated, indicating that alkaline stress caused the organism to utilize carbohydrates rather than proteins as much as possible to meet enhanced metabolism. The HIF pathway that maintains the body's survival was significantly up-regulated, at the expense of high energy consumption. Dysregulation of genes related to the formation of neutrophil extracellular traps (NETosis) pathway indicated that the immune function of the fish was suppressed. Overall, this study revealed the physiological and molecular responses of juvenile silver crucian carp to alkaline stress, deepening our understanding of their adaptation strategies and the potential impact of alkaline environments on aquatic life.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102393"},"PeriodicalIF":3.2,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142428595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Thiamine serves as a cofactor of key enzymes involved in glucose metabolism, and its regulatory role in high-carbohydrate diet has not been reported in crustaceans. In this study, six types of isonitrogen and isolipid diets were formulated at two carbohydrate levels (15 % corn starch for the low-carbohydrate group; 30 % corn starch for the high-carbohydrate group) and three concentrations of thiamine (0, 80 and 160 mg/kg) in Macrobrachium nipponense. The carbohydrate metabolism, antioxidant status, and mRNA expression of genes involved in thiamine transport and autophagy of prawns were investigated. The high-carbohydrate diet supplemented with 160 mg/kg thiamine increased the thiamine transporter 1 (SLC19A2) expression compared to a low-carbohydrate diet or other thiamine concentrations. Supplementation with 160 mg/kg thiamine under a high carbohydrate level significantly increased the pyruvate and lactate content in the hepatopancreas. When supplemented with either 0 or 160 mg/kg thiamine, a high dietary carbohydrate significantly increased glucose transporter 4 (GLUT4) and hexokinase (HK) mRNA expressions compared to a low-carbohydrate diet. Among the high-carbohydrate groups, prawns fed with 160 mg/kg thiamine showed significantly higher mRNA expression of pyruvate dehydrogenase-E1-α (PDH-E1-α) compared to those fed with 0 mg/kg thiamine. The activity of superoxide dismutase (SOD) was found to be highest in prawns fed 160 mg/kg thiamine. Supplementation of the carbohydrate diet with either 80 or 160 mg/kg thiamine led to a significant decrease in malondialdehyde (MDA) content accompanied by a significant decrease in glutathione (GSH) level, regardless of low or high carbohydrate levels. The unc-51 like autophagy activating kinase 1 (ULK1) mRNA expression was markedly influenced by both carbohydrate levels and thiamine concentration. Hence, the administration of 160 mg/kg thiamine can improve the thiamine transport, carbohydrate absorption, glycolysis and the antioxidant properties of M. nipponense when fed a high-carbohydrate diet.
{"title":"Dietary thiamine enhances thiamine transport, carbohydrate absorption, glycolysis, and antioxidant properties in Macrobrachium nipponense when fed a high-carbohydrate diet","authors":"Yonghui Jian , Jiaxin Song , Zhiyuan Chen, Dongsheng Zhou, Youqin Kong, Yujie Liu, Yixiang Zhang, Hongfeng Bai, Zhili Ding","doi":"10.1016/j.aqrep.2024.102400","DOIUrl":"10.1016/j.aqrep.2024.102400","url":null,"abstract":"<div><div>Thiamine serves as a cofactor of key enzymes involved in glucose metabolism, and its regulatory role in high-carbohydrate diet has not been reported in crustaceans. In this study, six types of isonitrogen and isolipid diets were formulated at two carbohydrate levels (15 % corn starch for the low-carbohydrate group; 30 % corn starch for the high-carbohydrate group) and three concentrations of thiamine (0, 80 and 160 mg/kg) in <em>Macrobrachium nipponense</em>. The carbohydrate metabolism, antioxidant status, and mRNA expression of genes involved in thiamine transport and autophagy of prawns were investigated. The high-carbohydrate diet supplemented with 160 mg/kg thiamine increased the thiamine transporter 1 (<em>SLC19A2</em>) expression compared to a low-carbohydrate diet or other thiamine concentrations. Supplementation with 160 mg/kg thiamine under a high carbohydrate level significantly increased the pyruvate and lactate content in the hepatopancreas. When supplemented with either 0 or 160 mg/kg thiamine, a high dietary carbohydrate significantly increased glucose transporter 4 (<em>GLUT4</em>) and hexokinase (<em>HK</em>) mRNA expressions compared to a low-carbohydrate diet. Among the high-carbohydrate groups, prawns fed with 160 mg/kg thiamine showed significantly higher mRNA expression of pyruvate dehydrogenase-E1-α (<em>PDH-E1-α</em>) compared to those fed with 0 mg/kg thiamine. The activity of superoxide dismutase (SOD) was found to be highest in prawns fed 160 mg/kg thiamine. Supplementation of the carbohydrate diet with either 80 or 160 mg/kg thiamine led to a significant decrease in malondialdehyde (MDA) content accompanied by a significant decrease in glutathione (GSH) level, regardless of low or high carbohydrate levels. The unc-51 like autophagy activating kinase 1 (<em>ULK1</em>) mRNA expression was markedly influenced by both carbohydrate levels and thiamine concentration. Hence, the administration of 160 mg/kg thiamine can improve the thiamine transport, carbohydrate absorption, glycolysis and the antioxidant properties of <em>M. nipponense</em> when fed a high-carbohydrate diet.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102400"},"PeriodicalIF":3.2,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142428101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study aimed to investigate the impact of dietary protein levels (DPLs) on the growth performance, plasma parameters, nutritional metabolism, and the mTOR pathway in Chinese perch. Three replicates of Chinese perch (34.39 ± 0.16 g) were provided with five diets containing varying protein levels (39.99 %, 43.09 %, 46.04 %, 49.14 %, and 52.12 %) over an 8-week period. The results revealed that, as DPLs increased, the final weight (FW), daily weight gain (DWG)), specific growth rate (SGR) and protein efficiency ratio (PER) initially rose and then declined, reaching their peak at 49.14 % DPL group. The feed conversion ratio (FCR) decreased first and then increased. In addition, compared to 46.04 % DPL group, the 39.99 % DPL group significantly suppressed whole body crude protein, liver crude protein, and liver crude lipid contents. The 52.12 % DPL group significantly increased liver crude lipid content and aspartate aminotransferase (AST) activity, and increased plasma total cholesterol (TC), total triglyceride (TG) and total lipase (TL) concentrations. However, in Chinese perch fed with 46.04 % DPL, the expression of genes associated with liver amino acid catabolism (ast, gdh and ampd), lipid hydrolysis, and oxidation (hsl, lpl and cs), as well as glycolysis (pk), was significantly increased. And the expression levels of mTOR pathway-related genes (s6k and mtor) and the phosphorylation level of p-S6 were significantly elevated in the liver of fish fed with 46.04 % DPL. In summary, a low DPL (39.99 %) significantly impedes the growth performance of Chinese perch, resulting in an elevated feed conversion ratio. And a high DPL (52.12 %) can induce lipid deposition in the liver of Chinese perch, increase metabolic stress, and lead to liver damage. Additionally, piecewise regression analysis and second-degree polynomial analysis revealed that optimal protein level in Chinese perch feed is suggested to range between 46.10 % and 48.71 %. This study provides partial theoretical basis for the design of commercial practical feed formulas for Chinese perch.
{"title":"Effects of dietary protein level on growth performance, plasma parameters, nutritional metabolism, and mTOR pathway of Chinese perch(Siniperca chuatsi)","authors":"Qiwei Zhang , Jiao Li , Liyun Ding , Yanping Zhang , Zhouming Qian , Xu-Fang Liang","doi":"10.1016/j.aqrep.2024.102390","DOIUrl":"10.1016/j.aqrep.2024.102390","url":null,"abstract":"<div><div>This study aimed to investigate the impact of dietary protein levels (DPLs) on the growth performance, plasma parameters, nutritional metabolism, and the mTOR pathway in Chinese perch. Three replicates of Chinese perch (34.39 ± 0.16 g) were provided with five diets containing varying protein levels (39.99 %, 43.09 %, 46.04 %, 49.14 %, and 52.12 %) over an 8-week period. The results revealed that, as DPLs increased, the final weight (FW), daily weight gain (DWG)), specific growth rate (SGR) and protein efficiency ratio (PER) initially rose and then declined, reaching their peak at 49.14 % DPL group. The feed conversion ratio (FCR) decreased first and then increased. In addition, compared to 46.04 % DPL group, the 39.99 % DPL group significantly suppressed whole body crude protein, liver crude protein, and liver crude lipid contents. The 52.12 % DPL group significantly increased liver crude lipid content and aspartate aminotransferase (AST) activity, and increased plasma total cholesterol (TC), total triglyceride (TG) and total lipase (TL) concentrations. However, in Chinese perch fed with 46.04 % DPL, the expression of genes associated with liver amino acid catabolism (<em>ast</em>, <em>gdh</em> and <em>ampd</em>), lipid hydrolysis, and oxidation (<em>hsl</em>, <em>lpl</em> and <em>cs</em>), as well as glycolysis (<em>pk</em>), was significantly increased. And the expression levels of mTOR pathway-related genes (<em>s6k</em> and <em>mtor</em>) and the phosphorylation level of p-S6 were significantly elevated in the liver of fish fed with 46.04 % DPL. In summary, a low DPL (39.99 %) significantly impedes the growth performance of Chinese perch, resulting in an elevated feed conversion ratio. And a high DPL (52.12 %) can induce lipid deposition in the liver of Chinese perch, increase metabolic stress, and lead to liver damage. Additionally, piecewise regression analysis and second-degree polynomial analysis revealed that optimal protein level in Chinese perch feed is suggested to range between 46.10 % and 48.71 %. This study provides partial theoretical basis for the design of commercial practical feed formulas for Chinese perch.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102390"},"PeriodicalIF":3.2,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142428100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-01DOI: 10.1016/j.aqrep.2024.102386
Yuru Zhang , Haiying Fan , Kedi Gao , Shibo Zhang , Ronghua Lu , Xianglin Cao , Xinxin Xu , Jiancheng Liu
The grass carp (Ctenopharyngodon idella) holds significant economic value worldwide. However, intensive farming practices often result in lipid metabolic disorder of fish. It is essential to explore the influence of pivotal genes in the process of fat accumulation, particularly from the perspective of fat transport. Fatty acid binding proteins (FABPs) are crucial for selectively binding and transporting free fatty acids (FAs) within cells. Nevertheless, the types of fabp genes in the grass carp genome and their responses to various FAs remain unclear. In the current study, exhaustive bioinformatic analysis identified nine fabp genes in the grass carp genome: fabp1a, fabp1b.1, fabp2, fabp3, fabp6, fabp7a, fabp10a, fabp11a, and fabp11b. Although these genes showed widespread expression across various tissues, each gene displayed distinct preferences for specific primary expression tissues. Notably, fabp1a exhibited prominent expression in muscle, fabp1b.1 in abdominal fat, fabp2 and fabp6 in the intestine, fabp7a in the brain, fabp10a in the hepatopancreas, and fabp11a showed maximal expression in the eye. Findings from primary grass carp hepatocytes revealed that the addition of palmitic acid (PA), high concentrations oleic acid (OA) and docosahexaenoic acid (DHA) (400 µM), significantly increased intracellular lipid accumulation. Intriguingly, the addition of PA resulted in increased expression of fabp2 and fabp11a, while DHA significantly enhanced the expression of fabp7a (P < 0.01). Given that the accumulated FAs are primarily saturated fatty acids, these results suggest that regulating fabps, especially fabp2 and fabp11a, could be a strategy to control fat deposition in grass carp.
{"title":"Identification, tissue expression, and response to various fatty acid inductions of the fabp gene family in grass carp (Ctenopharyngodon idella)","authors":"Yuru Zhang , Haiying Fan , Kedi Gao , Shibo Zhang , Ronghua Lu , Xianglin Cao , Xinxin Xu , Jiancheng Liu","doi":"10.1016/j.aqrep.2024.102386","DOIUrl":"10.1016/j.aqrep.2024.102386","url":null,"abstract":"<div><div>The grass carp (<em>Ctenopharyngodon idella</em>) holds significant economic value worldwide. However, intensive farming practices often result in lipid metabolic disorder of fish. It is essential to explore the influence of pivotal genes in the process of fat accumulation, particularly from the perspective of fat transport. Fatty acid binding proteins (FABPs) are crucial for selectively binding and transporting free fatty acids (FAs) within cells. Nevertheless, the types of <em>fabp</em> genes in the grass carp genome and their responses to various FAs remain unclear. In the current study, exhaustive bioinformatic analysis identified nine <em>fabp</em> genes in the grass carp genome: <em>fabp1a, fabp1b.1, fabp2, fabp3, fabp6, fabp7a, fabp10a, fabp11a,</em> and <em>fabp11b</em>. Although these genes showed widespread expression across various tissues, each gene displayed distinct preferences for specific primary expression tissues. Notably, <em>fabp1a</em> exhibited prominent expression in muscle, <em>fabp1b.1</em> in abdominal fat, <em>fabp2</em> and <em>fabp6</em> in the intestine, <em>fabp7a</em> in the brain, <em>fabp10a</em> in the hepatopancreas, and <em>fabp11a</em> showed maximal expression in the eye. Findings from primary grass carp hepatocytes revealed that the addition of palmitic acid (PA), high concentrations oleic acid (OA) and docosahexaenoic acid (DHA) (400 µM), significantly increased intracellular lipid accumulation. Intriguingly, the addition of PA resulted in increased expression of <em>fabp2</em> and <em>fabp11a</em>, while DHA significantly enhanced the expression of <em>fabp7a</em> (<em>P</em> < 0.01). Given that the accumulated FAs are primarily saturated fatty acids, these results suggest that regulating <em>fabps</em>, especially <em>fabp2</em> and <em>fabp11a,</em> could be a strategy to control fat deposition in grass carp.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102386"},"PeriodicalIF":3.2,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142428644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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