Aquaculture Reports最新文献

{"title":"Effects of probiotic and antibiotic on gut microbiome and antibiotic resistance genes in black tiger shrimp (Penaeus monodon) in Bangladesh","authors":"Shrabonti Halder ,&nbsp;Shankar C. Mandal ,&nbsp;Goutam Kumar Kundu ,&nbsp;Anwar Hossain ,&nbsp;Md. Golam Rabbane ,&nbsp;Asif Rahman ,&nbsp;Abdullah-Al Mamun ,&nbsp;Md. Latiful Islam ,&nbsp;Kozo Watanabe ,&nbsp;Md. Inja-Mamun Haque","doi":"10.1016/j.aqrep.2025.103294","DOIUrl":"10.1016/j.aqrep.2025.103294","url":null,"abstract":"<div><div>Black tiger shrimp <em>(Penaeus monodon)</em> is the major brackish-water aquaculture species in Bangladesh and other parts of the world. The use of antibiotics and probiotics in shrimp cultivation could potentially impact the gut microbiome, leading to the emergence of antibiotic resistance genes (ARGs) in aquaculture environments. This research investigated the dynamics of shrimp gut microbial community structure and the prevalence of ARGs over a 28-day period. Four treatments were included: control, probiotics (<em>Bacillus</em>), antibiotics (oxytetracycline), and a combination of probiotics and antibiotics. The microbiome was determined using the 16S rRNA metabarcoding analysis, and 19 ARGs were detected using PCR. The microbiome analysis revealed that <em>Bacillus</em> and <em>Acinetobacter</em> were initially abundant in the groups, and changes in the genus composition were observed over the treatment period. The temporal changes in community structure in the antibiotic treatment group suggested that while antibiotics may initially impact on the bacterial community, the community may eventually recover and become similar to the other treatment groups. The combined treatment of antibiotics and probiotics resulted in an initial equilibrium between <em>Firmicutes</em> and <em>Proteobacteria</em> on day 8, followed by a subsequent transition toward <em>Firmicutes</em> dominance, probably because of the continued presence of the probiotic bacteria. The antibiotic treatment revealed the highest number of ARGs (<em>tetA, tetC, sul1, bacA,</em> and <em>ere(A)</em>), followed by the control group <em>(tetA, tetC, ere(A),</em> and <em>bacA</em>). Three ARGs (<em>tetA, tetC,</em> and <em>bacA</em>) and two (<em>tetA</em> and <em>dfrA1</em>) were detected in the probiotic and mixed treatment groups, respectively. No ARGs were detected from the mixed treatment group at the end of the treatment. These findings suggest that the simultaneous use of antibiotics and probiotics may potentially reduce the emergence of ARGs in shrimp farming. The findings may contribute to the development of strategies for using probiotics and antibiotics in shrimp farming and addressing the issue of antibiotic resistance in the aquaculture sector.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"46 ","pages":"Article 103294"},"PeriodicalIF":3.7,"publicationDate":"2025-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145787128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spatial Genetic Structuring and Divergence in Labeo rohita Across South Asian Rivers: Insights for Conservation Management","authors":"Md. Mahfuzur Rahman ,&nbsp;Khandaker Asif Ahmed ,&nbsp;Rowshan Ara Begum ,&nbsp;Mohammad Shamimul Alam","doi":"10.1016/j.aqrep.2025.103280","DOIUrl":"10.1016/j.aqrep.2025.103280","url":null,"abstract":"<div><div>Understanding how large river networks shape genetic variation is critical for conserving freshwater biodiversity in the regions under ecological and socio-economic pressures. In South Asia, the rohu carp <em>Labeo rohita</em> is one of the most popular freshwater fish. Yet, the diversity of its population structure across rivers of different countries remains poorly understood. Here, we integrate mitochondrial <em>cytb</em> sequences of <em>L. rohita</em> originating from Bangladeshi rivers with those from Indian rivers to assess genetic diversity, spatial structure, and divergence across eight rivers. Analyses of 308 individuals revealed two divergent genetic clusters: one comprising eastern rivers (Ganga, Padma, Jamuna, Halda) and the other including western and northern rivers (Satluj, Rapti, Mahanadi, Brahmaputra). Despite its location in India, the Ganga population clustered with Bangladeshi rivers, consistent with historical hydrological connectivity, while the Satluj population showed the highest genetic divergence, likely reflecting long-term isolation in the Indus basin. The Ganga population also exhibited notably low haplotype and nucleotide diversity, suggesting genetic erosion due to decades of unregulated hatchery stocking. Bayesian clustering, AMOVA, and pairwise FST analyses confirmed strong spatial structuring (FST = 0.203, p &lt; 0.001), and isolation-by-distance patterns indicated a relationship between distance and gene flow. A median-joining haplotype network revealed a star-like topology, suggestive of recent demographic expansion. Phylogenetic reconstruction supported recognition of eastern and western lineages as distinct management units. These findings highlight the need for basin-specific broodstock management, genetic monitoring, and international conservation strategies to sustain genetic diversity in this economically important freshwater species.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"46 ","pages":"Article 103280"},"PeriodicalIF":3.7,"publicationDate":"2025-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145787130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of mollusks as diets on mud crab Scylla paramamosain：Growth performance and muscle quality","authors":"Haoji Guo ,&nbsp;Haoran Zhang ,&nbsp;Huaming Liu ,&nbsp;Jiawei Wang ,&nbsp;Mingfei Feng ,&nbsp;Baojiao Chen ,&nbsp;Shaojian Chen ,&nbsp;Kun Wu ,&nbsp;Xiaobo Wen","doi":"10.1016/j.aqrep.2025.103292","DOIUrl":"10.1016/j.aqrep.2025.103292","url":null,"abstract":"<div><div>The mud crab <em>Scylla paramamosain</em> is the largest marine-cultured crab in China, with prolific meat yield, unique flavor, and high nutritional content. The prevailing negative perceptions among farmers regarding the adaptability of mud crab to pelletized feeds have resulted in a continued reliance on natural diet. However, little is known about the effects on growth and muscle quality of mud crab fed natural diets. This study investigated the growth performance, textural properties and flavor indicators in muscle of <em>Scylla paramamosain</em> fed five commonly available low-value mollusks, such as <em>Potamocorbula amurensis, Corbicula fluminea, Perna viridis, Oncomelania hupensis,</em> and <em>Cipangopaludina chinensis</em>. After a 60-day trial, <em>Scylla paramamosain</em> fed <em>Potamocorbula amurensis</em> had the highest growth performance compared to those consuming other mollusks, particularly specific growth rate, and protein efficiency ratio. Texture analysis indicated that mud crab fed <em>Potamocorbula amurensis</em> enhanced the hardness, chewiness, and gumminess in muscle. Additionally, flavor profiling revealed significantly elevated umami and sweet levels in muscle of mud crab fed <em>Potamocorbula amurensis</em>. Collectively, these findings not only confirmed that <em>Potamocorbula amurensis</em> was a suitable natural diet for mud crab <em>Scylla paramamosain</em>, promoting both growth performance and muscle quality, but also provided a foundation for future explorations into the development of formulated diet in economically important crustacean species.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"46 ","pages":"Article 103292"},"PeriodicalIF":3.7,"publicationDate":"2025-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145787043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histological chronology and thermosensitive period of gonadal sex differentiation in turbot (Scophthalmus maximus)","authors":"Zhen Meng ,&nbsp;Luyao Cheng ,&nbsp;Peng Hu ,&nbsp;Xinfu Liu ,&nbsp;Hesen Zhang ,&nbsp;Bin Liu ,&nbsp;Zhi Yang","doi":"10.1016/j.aqrep.2025.103307","DOIUrl":"10.1016/j.aqrep.2025.103307","url":null,"abstract":"<div><div>The turbot (<em>Scophthalmus maximus</em>), an economically significant marine flatfish with a ZW/ZZ sex determination system, exhibits marked sexual growth dimorphism favoring females, making all-female culture a key aquaculture objective. However, effective sex control requires precise understanding of gonadal differentiation timing and environmental influences. This study combined detailed histological analysis with systematic temperature manipulation to establish the chronology of gonadal development and identify the thermosensitive window for sex determination. Histological examination revealed that ovarian differentiation, marked by cavity formation, commenced at 45 days post-hatching (dph) (37.4 mm TL), preceding the appearance of meiotic oocytes at 65 dph. Testicular differentiation occurred later at 65 dph (50.4 mm TL), demonstrating delayed development compared to ovaries. Through controlled temperature exposures at different developmental stages, we identified a critical thermosensitive window spanning 28–54 dph (20.05–43.00 mm TL), with the most sensitive period confined to 28–40 dph (20.05–30.11 mm TL). Exposure to 25°C during this window induced significant masculinization, yielding up to 74.3 % phenotypic males, while maintaining 18°C throughout this period not only preserved a balanced 1:1 sex ratio but also supported optimal growth performance. These findings establish that the thermosensitive period precedes histological gonadal differentiation, indicating that sex determination in turbot occurs earlier than previously recognized and is strongly influenced by temperature during a defined developmental window. Our results provide crucial insights for developing temperature-based sex control protocols, enabling reliable production of all-female populations through precise thermal management during early juvenile stages.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"46 ","pages":"Article 103307"},"PeriodicalIF":3.7,"publicationDate":"2025-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145787470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-omics insights into inulin mediated enhancement of the large yellow croaker (Larimichthys crocea) growth during the summer season heat stress","authors":"Xiaolong Yin ,&nbsp;Chengzhang Huang ,&nbsp;Kai Xu ,&nbsp;Huirong Lv ,&nbsp;Zhangjie Chu ,&nbsp;Jungyeol Park ,&nbsp;Junwook Hur ,&nbsp;Yang Gao ,&nbsp;Weiye Li","doi":"10.1016/j.aqrep.2025.103297","DOIUrl":"10.1016/j.aqrep.2025.103297","url":null,"abstract":"<div><div>Elevated mortality rates and growth suppression of large yellow croaker (<em>Larimichthys crocea</em>) under prolonged high summer temperatures present significant challenges for sustainable aquaculture. In response to these thermal stress pressures, this study investigates the effects of dietary inulin supplementation on the resilience and growth performance of juvenile large yellow croaker. Over a two-month period (July 14 to September 14), fish were acclimated and assigned to five diet groups containing 0 %, 0.1 %, 0.2 %, 0.4 %, and 0.8 % inulin. Using a multi-omics approach (transcriptomic, metabolomic, and microbiomic changes) in liver and gut tissues, we assessed the effect of inulin in <em>Larimichthys crocea</em>. Results demonstrated that an optimal inulin level (0.4 %) significantly improved growth performance. Transcriptomic data revealed upregulation of genes involved in energy metabolism pathways such as glycolysis and fatty acid biosynthesis, alongside downregulation of immune and stress-related genes. Metabolomic profiles showed increased metabolites related to energy production and lipid metabolism. Gut microbiome analysis indicated a reduction in overall diversity but enrichment of beneficial <em>bacteria</em>, including <em>Lactobacillus</em>, which correlated with higher levels of health-promoting short-chain fatty acids and amino acids. Integrated analyses highlighted strong associations between key microbial taxa and metabolic pathways supporting nutrient absorption and vitamin synthesis. These findings suggest that dietary inulin enhances thermal stress tolerance by modulating metabolism and gut microbiota, offering a promising nutritional strategy to improve large yellow croaker aquaculture under change-induced heat stress.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"46 ","pages":"Article 103297"},"PeriodicalIF":3.7,"publicationDate":"2025-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145787041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shrimp hydrolysate-based palatability enhancer: A strategy to reduce fish-in fish-out ratio in marine fish species","authors":"Mikaël Herault ,&nbsp;Buddhi E. Gunathilaka ,&nbsp;Vincent Fournier ,&nbsp;Hervé Le Bris ,&nbsp;Kyeong-Jun Lee ,&nbsp;Bastien Sadoul","doi":"10.1016/j.aqrep.2025.103291","DOIUrl":"10.1016/j.aqrep.2025.103291","url":null,"abstract":"<div><div>Reducing aquaculture’s reliance on wild fish remains a major challenge. Protein hydrolysates, often derived from food by-products, offer a promising, sustainable, and functional alternative to marine proteins. This study evaluated their use as a palatability enhancer (PE) by top-coating feeds with a low-dose liquid hydrolysate, primarily from shrimp, to reduce fish meal (FM) in juvenile red seabream (<em>Pagrus major</em>) diets. Experimental feeds included a high FM diet (HFM, 30 % FM), a low FM diet (LFM, 15 % FM) and two additional diets with 2 % PE top-coated (HFM+PE and LFM+PE). Six replicate tanks, each with 40 fish, were fed one of the diets twice daily to satiety for 15 weeks. Half the tanks experienced intermittent stress (1-min net chasing for one week, every 3 weeks). After the trial, fish were challenged with <em>Edwardsiella tarda</em>. Both basal diets resulted in comparable growth and feed efficiency. Reducing fish meal from 30 % to 15 % in the LFM diet resulted in a baseline reduction of approximately 25 % in wild fish use, as estimated by the economic Fish-In: Fish-Out (eFIFO) ratio. PE supplementation further improved growth (+5.6 % for SGR), health, and feed utilization (-7.5 % for FCR), enabling an additional 6 % reduction in wild-caught fish input compared to the LFM diet alone. The beneficial effects of PE were consistent despite intermittent stress, and survival following bacterial challenge was significantly higher with PE supplementation. In conclusion, liquid hydrolysate used as a PE provides a cost-effective way to reduce pressure on wild fish stocks while maintaining high feed performance.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"46 ","pages":"Article 103291"},"PeriodicalIF":3.7,"publicationDate":"2025-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145787045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of dietary oxidized rice bran on the coloration, antioxidant capacity, and immunity in channel catfish (Ictalurus punctatus)","authors":"Chunyu Xue ,&nbsp;Leimin Zhang ,&nbsp;Haifeng Mi ,&nbsp;Dongyu Huang ,&nbsp;Hualiang Liang ,&nbsp;Mingchun Ren ,&nbsp;Lu Zhang","doi":"10.1016/j.aqrep.2025.103287","DOIUrl":"10.1016/j.aqrep.2025.103287","url":null,"abstract":"<div><div>To examine the impact of oxidized rice bran on body pigmentation and immune-antioxidant responses in channel catfish, four diets containing rice bran with varying oxidation levels were formulated for a 9-week feeding trial. The oxidation values were 1.28 mmol/kg (Control), 12.9 mmol/kg (TSR1), 24.3 mmol/kg (TSR2), and 36.7 mmol/kg (TSR3). Compared with the control, TSR3 exhibited a significantly reduced FCR, while plasma AST and ALT levels were markedly elevated in TSR2 and TSR3. Regarding skin pigmentation indices, L* values were significantly lower in TSR1 and TSR2, whereas a* values increased in TSR1–TSR3 and b* values in TSR2–TSR3. In muscle tissue, a* value was significantly higher in TSR3, and b* values in TSR1–TSR3. Both skin and muscle uranidin contents rose significantly in TSR1–TSR3, accompanied by a decline in tyrosinase levels that paralleled the reduction in muscle melanin content. Skin melanin concentration was significantly reduced in TSR2–TSR3. The expression of pigmentation-related genes (<em>mitf</em>, <em>tyr</em>, <em>α-msh</em>, <em>etb</em>, and <em>camk2</em>) in skin and muscle was markedly suppressed in TSR1–TSR3, while <em>xdh</em> expression was up-regulated. Concerning antioxidant parameters, MDA concentrations in skin and muscle were elevated in TSR1–TSR3, whereas T-AOC levels and the activities of skin T-SOD, GPx, and muscle CAT declined significantly. Skin CAT and muscle GPx activities were further reduced in TSR2–TSR3. Gene expression analyses indicated significant down-regulation of <em>ifn-γ</em>, <em>nrf2</em>, and <em>il-10</em> in skin and muscle, as well as <em>gpx</em> in muscle within TSR1–TSR3, alongside increased <em>keap1</em> and <em>nfκb</em> expression in skin. In TSR2–TSR3, <em>gpx</em> expression in skin was significantly decreased, and <em>keap1</em> and <em>nfκb</em> were up-regulated in muscle. Overall, oxidized rice bran lowered FCR but adversely affected pigmentation, antioxidant defenses, and immune function in channel catfish</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"46 ","pages":"Article 103287"},"PeriodicalIF":3.7,"publicationDate":"2025-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145787042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The food digestion strategies of three wild cold-water adult fishes","authors":"Liangliang Xu ,&nbsp;Peng Xiang ,&nbsp;Sihai Min ,&nbsp;Baowen Zhang ,&nbsp;Hanxi Chen ,&nbsp;Zesong Wang ,&nbsp;Yanjun Jin ,&nbsp;Fenglin Liu ,&nbsp;Xiaoshuai Liu ,&nbsp;Zhaobin Song","doi":"10.1016/j.aqrep.2025.103293","DOIUrl":"10.1016/j.aqrep.2025.103293","url":null,"abstract":"<div><div>Herbivorous, omnivorous, and carnivorous fish represent the predominant trophic levels in cold-water ecosystems. These groups exhibit differences in food composition and nutrient intake based on their respective trophic positions. Understanding the mechanisms of food digestion in cold-water fish is crucial for effective population conservation and restoration efforts. This study employed several approaches, including food nutrient composition analysis, 16S rRNA gene sequencing, and metagenomics, to investigate the food digestion mechanisms in <em>Schizothorax wangchiachii</em> (herbivorous), <em>Schizothorax kozlovi</em> (omnivorous), and <em>Percocypris pingi</em> (carnivorous). A total of 45 gut samples of these three cold-water adult fishes were collected from the Jinping Dahewan range of lower reaches of the Yalong River. Significant differences were observed in the nutritional composition of the food consumed by these three cold-water fish species (Kruskal-Wallis H test; <em>P</em> &lt; 0.05). The proportion of protein in the diets of cold-water fish increased significantly from 65 % in <em>S. wangchiachii</em> to 98 % in <em>P. pingi</em>. The 16S rRNA gene sequencing revealed that Proteobacteria constituted the highest relative abundance in the gut of <em>P. pingi</em> (56 %), followed by <em>S. wangchiachii</em> (39 %), while <em>S. kozlovi</em> exhibited the lowest relative abundance at 30 %. Metagenomics analysis of the gut microbiota indicated that the intestinal microorganisms of <em>S. wangchiachii</em> and <em>S. kozlovi</em> were primarily enriched in cellulose and hemicellulose degradation pathways, whereas <em>P. pingi</em> was predominantly enriched in amino acid metabolism. Notably, the microbial flora of <em>S. wangchiachii</em> had the highest number of genes associated with xylanase, 1,4-β-xylosidase, and cellulase compared to <em>S. kozlovi</em> and <em>P. pingi</em>. Additionally, proteins such as homoserine/homoserine lactone efflux protein (K05834), as well as conjugal transfer pilus assembly protein TrbC (K12059), showed the highest gene abundance in the gut of <em>P. pingi</em>. Overall, this study serves as an important reference for understanding food digestion in cold-water fish.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"46 ","pages":"Article 103293"},"PeriodicalIF":3.7,"publicationDate":"2025-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145787127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated transcriptome and metabolome analysis reveals the effect of kelp on lipid metabolism in the liver of large yellow croaker (Larimichthys crocea)","authors":"Qiu-Meng Xiang ,&nbsp;Hui-Xiong Liu ,&nbsp;Jian-Ping Wang ,&nbsp;Cong-Cong Hou","doi":"10.1016/j.aqrep.2025.103284","DOIUrl":"10.1016/j.aqrep.2025.103284","url":null,"abstract":"<div><div><em>Larimichthys crocea</em> is an important maricultured fish in China, yet the quality of its cultured counterparts is often inferior to that of wild fish, primarily due to the hepatic lipid accumulation induced by high-fat aquaculture diets. Given that studies have shown dietary kelp can significantly reduce hepatic lipid deposition in fish, this study explored its potential to ameliorate hepatic lipid accumulation in <em>L. crocea</em> as a quality improvement strategy. Fish were fed a 3 % kelp diet for 8 weeks. Using histology, qPCR, enzyme tests, and integrated transcriptomics/metabolomics, we elucidated the lipid-lowering mechanism of kelp and its impact on liver physiology. The results demonstrated that kelp reduced hepatic lipid content. Moreover, it could upregulate <em>leptin</em>, reduce energy intake, and increase energy expenditure. Concurrently, key lipid anabolism genes (<em>FADS2</em>, <em>FASN</em>, <em>ELOVL6</em>, <em>Acaca</em>, <em>LPIN1</em>) were downregulated, indicating inhibited lipid synthesis. Furthermore, reduced lipid load significantly downregulated lipid-catabolism genes (<em>ACOX1</em>, <em>PPAR</em>α), and this physiological feedback subsequently inhibited lipid degradation effectively. Integrated analyses showed kelp enhanced unsaturated fatty acid (UFA) content. Importantly, kelp supplementation decreased the activity of AST, downregulated ferroptosis-associated genes (<em>acsl1a</em>, <em>acsl5</em>), and upregulated the ferroptosis-inhibiting gene <em>GPX4</em> and stress-resistance genes (<em>HSP70</em>, <em>HSP90</em>), suggesting enhanced hepatic stress resistance. In conclusion, kelp-supplemented feed reduces hepatic lipid content in <em>L. crocea</em> by decreasing energy intake and inhibiting lipid synthesis, while simultaneously increasing UFAs content, achieving both lipid reduction and nutritional enhancement. This study provides an important theoretical basis for the application of kelp-supplemented functional feed in <em>L. crocea</em> aquaculture.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"46 ","pages":"Article 103284"},"PeriodicalIF":3.7,"publicationDate":"2025-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145787126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Towards overcoming shrimp maturation challenges in aquaculture production using multi-omics approaches: A review in captive penaeid shrimp","authors":"Kanchana Sittikankaew ,&nbsp;Umaporn Uawisetwathana ,&nbsp;Wirulda Pootakham ,&nbsp;Nitsara Karoonuthaisiri","doi":"10.1016/j.aqrep.2025.103301","DOIUrl":"10.1016/j.aqrep.2025.103301","url":null,"abstract":"<div><div>Shrimp is a major global aquatic product, contributing significantly to both food security and the economy. Despite the increasing global production of farmed shrimp, challenges such as disease outbreaks, size variation, and limited reproductive success remain significant barriers to sustainable shrimp aquaculture. Improving reproductive maturation in captivity is therefore a key determinant for the long-term sustainability of the shrimp industry. Advancements in omics technologies, including transcriptomics, proteomics, metabolomics, and integrated multi-omics approach, have revolutionized our understanding of the complex processes governing shrimp reproduction. This review examines multi-omics studies from 2014 to 2025 that investigate shrimp reproductive maturation in different angles ranging from hormonal and genetic regulation impacts, dietary influences, and environmental factors. These studies have identified key hormonal pathways, tissue-specific markers, and regulatory genes associated with gonadal maturation in both intrinsic (genetic) and extrinsic (nutrients and environments) influences. The molecular mechanisms revealed by these advanced multi-omics studies enable targeted management of shrimp farming such as feed formulations optimization and aquaculture system development. Future perspectives highlight the need to harness insights from multi-omics approaches to promote shrimp maturation through sustainable practices, thereby eliminating invasive methods such as eyestalk ablation. Moreover, the current lack of comprehensive molecular databases encompassing nucleotide, protein, and metabolite information remains a critical bottleneck. Expanding and openly sharing these resources will be essential to maximize the power of integrated omics and accelerate innovation in shrimp aquaculture.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"46 ","pages":"Article 103301"},"PeriodicalIF":3.7,"publicationDate":"2025-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145787129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}