Apolipoprotein A-IV (ApoA-IV) is a multifunctional protein that participates in a broad spectrum of biological processes, including the modulation of immune function. Nevertheless, the specific roles of ApoA-IV in the immune response to acute bacterial infections in Nile tilapia (Oreochromis niloticus) are yet to be fully elucidated. In the present study, we identified ApoA-IV from O. niloticus (On-ApoA-IV) and examined its role in bacterial infections. The open reading frame of On-ApoA-IV spanned 768 bp and encoded a sequence of 255 amino acids. Its transcriptional abundance was the highest in the intestine, and On-ApoA-IV was induced in tilapia challenged with Streptococcus agalactiae. Additionally, experimental findings indicated that On-ApoA-IV can inhibit inflammatory processes and apoptosis, thereby enhancing tilapia survival during acute bacterial infection. The current data provide a theoretical foundation for further elucidation of the mechanisms by which ApoA-IV safeguard fish against pathogens.
{"title":"Cloning and characterization of apolipoprotein A-IV (ApoA-IV) from Oreochromis niloticus involved in immune defense functions","authors":"Yongxiong Huang , Xuyan Tan , Jiamin Feng , Zhiqiang Zhang , Kwaku Amoaha , Berchie Asiedu , Yu Huang , Jichang Jian","doi":"10.1016/j.aqrep.2024.102355","DOIUrl":"10.1016/j.aqrep.2024.102355","url":null,"abstract":"<div><div>Apolipoprotein A-IV (ApoA-IV) is a multifunctional protein that participates in a broad spectrum of biological processes, including the modulation of immune function. Nevertheless, the specific roles of ApoA-IV in the immune response to acute bacterial infections in Nile tilapia (<em>Oreochromis niloticus</em>) are yet to be fully elucidated. In the present study, we identified <em>ApoA-IV</em> from <em>O. niloticus</em> (On-ApoA-IV) and examined its role in bacterial infections. The open reading frame of On-ApoA-IV spanned 768 bp and encoded a sequence of 255 amino acids. Its transcriptional abundance was the highest in the intestine, and <em>On-ApoA-IV</em> was induced in tilapia challenged with <em>Streptococcus agalactiae</em>. Additionally, experimental findings indicated that On-ApoA-IV can inhibit inflammatory processes and apoptosis, thereby enhancing tilapia survival during acute bacterial infection. The current data provide a theoretical foundation for further elucidation of the mechanisms by which ApoA-IV safeguard fish against pathogens.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102355"},"PeriodicalIF":3.2,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142324074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shelters are widely used in sea cucumber aquaculture. But the long-term effects of shelters on behavior and physiology are unknown in sea cucumber aquaculture. In this research, the number of grasping of the sea cucumber mouth tentacle significantly increased in the experimental group (group Y) compared with the control group (group C) (P = 0.003), indicating that the shelter increases the feeding rate of sea cucumbers. The height of the intestinal crease in group Y was significantly higher than that in group C (P < 0.001), and the proportion of Patescibacteria in the intestine significantly increased. However, there was no significant difference in digestive enzyme activities, and the defecation rate of Apostichopus japonicus in group Y was significantly lower than that in group C (P = 0.047). This suggests that the shelter probably improves the digestive capacity of sea cucumbers by altering their gut microbial composition and gut structure. In addition, final body weight (P = 0.006) and daily gain (P = 0.008) in group Y were significantly higher than those in group C. This indicates that the shelter improves the growth efficiency of sea cucumbers. The present study reveals that long-term use of shelter greatly improves feeding behavior, defecation behavior, gut microbiota composition, gut structure, and growth of A. japonicus. In summary, the shelter improves the behavior and physiological activities of sea cucumbers, and consequently increases the aquaculture efficiency.
遮蔽物在海参养殖中被广泛使用。但在海参养殖中，遮蔽物对行为和生理的长期影响尚不清楚。在本研究中，实验组（Y 组）与对照组（C 组）相比，海参口触角的抓取次数明显增加（P = 0.003），表明遮蔽物提高了海参的摄食率。Y 组的肠道皱襞高度明显高于 C 组（P < 0.001），肠道中的贝特氏菌比例明显增加。但消化酶活性无明显差异，Y 组日本豚的排便率明显低于 C 组（P = 0.047）。这表明，庇护所可能通过改变海参的肠道微生物组成和肠道结构来提高其消化能力。此外，Y 组的最终体重（P = 0.006）和日增重（P = 0.008）显著高于 C 组。本研究表明，长期使用遮蔽物可大大改善日本海参的摄食行为、排便行为、肠道微生物群组成、肠道结构和生长。总之，遮蔽物改善了海参的行为和生理活动，从而提高了养殖效率。
{"title":"Effects of artificial shelter on feeding, digestion, and growth of the sea cucumber Apostichopus japonicus","authors":"Xiang Li, Xiajing Wang, Zihe Zhao, Yingzhuo Sun, Jun Ding, Yaqing Chang, Chong Zhao","doi":"10.1016/j.aqrep.2024.102379","DOIUrl":"10.1016/j.aqrep.2024.102379","url":null,"abstract":"<div><div>Shelters are widely used in sea cucumber aquaculture. But the long-term effects of shelters on behavior and physiology are unknown in sea cucumber aquaculture. In this research, the number of grasping of the sea cucumber mouth tentacle significantly increased in the experimental group (group Y) compared with the control group (group C) (<em>P</em> = 0.003), indicating that the shelter increases the feeding rate of sea cucumbers. The height of the intestinal crease in group Y was significantly higher than that in group C (<em>P</em> < 0.001), and the proportion of Patescibacteria in the intestine significantly increased. However, there was no significant difference in digestive enzyme activities, and the defecation rate of <em>Apostichopus japonicus</em> in group Y was significantly lower than that in group C (<em>P</em> = 0.047). This suggests that the shelter probably improves the digestive capacity of sea cucumbers by altering their gut microbial composition and gut structure. In addition, final body weight (<em>P</em> = 0.006) and daily gain (<em>P</em> = 0.008) in group Y were significantly higher than those in group C. This indicates that the shelter improves the growth efficiency of sea cucumbers. The present study reveals that long-term use of shelter greatly improves feeding behavior, defecation behavior, gut microbiota composition, gut structure, and growth of <em>A. japonicus.</em> In summary, the shelter improves the behavior and physiological activities of sea cucumbers, and consequently increases the aquaculture efficiency.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102379"},"PeriodicalIF":3.2,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142319807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-24DOI: 10.1016/j.aqrep.2024.102366
Jinghua Chen , Chao Sui , Yacheng Hu , Huimin Qin , Danyang Zhang , Jiang Wei , Boji Cao , Qingfei Li
This study aimed to investigate the effects of dietary phospholipids (PLs) on growth performance, antioxidant capacity, and lipid metabolism of juvenile Chinese sturgeon (Acipenser sinensis). Juvenile sturgeon, with an initial body weight of 12.27±0.03 g, were fed five diets containing graded levels of PLs (1.26 %, 3.29 %, 4.34 %, 5.93 %, and 7.02 %) in triplicate for 56 days. All experimental diets were formulated to be isonitrogenous (55 %) and isolipidic (10 %). Results indicated that specific growth rate increased from 2.21 to 2.89 with elevated levels of dietary PLs. The optimum PLs level for juvenile Chinese sturgeon was estimated to be 5.93 % of diet according to the broken-line regression analysis of the specific growth rate against dietary PLs levels. Diets containing higher levels of PLs (5.93 % and 7.02 % of diet) significantly reduced the lipid content of whole body and liver by around 30 % in juvenile sturgeon compared to the control group (P <0.05). The proportions of specific fatty acids present in the liver lipid were linearly correlated with their percentages in dietary lipid (P <0.05), whereas muscle fatty acid composition remained unaffected across different treatments (P >0.05). EPA and DHA were preferentially accumulated into the muscle tissue. Activities of digestive enzymes and antioxidant enzymes were linearly increased with incremental dietary PLs levels (P <0.001). Inclusion of PLs in diet significantly enhanced the expression of antioxidant genes with the transcription of GSH-Px in the liver of fish fed 7.02 % PLs of diet being approximately 8-fold greater than in those fish fed diet without PL addition (P <0.001). Dietary PLs significantly induced the gene expression related to lipid catabolism, and suppressed the transcription of genes involved in lipogenesis (P <0.001). Based on growth performance, antioxidant capacity, and liver biochemical parameters, inclusion of 5.93 % PLs (soy lecithin preparation) in the diets is recommend for juvenile Chinese sturgeon.
{"title":"Effects of dietary phospholipids on growth performance, antioxidant capacity, and lipid metabolism of juvenile Chinese sturgeon (Acipenser sinensis), a critically endangered sturgeon in the Yangtze River","authors":"Jinghua Chen , Chao Sui , Yacheng Hu , Huimin Qin , Danyang Zhang , Jiang Wei , Boji Cao , Qingfei Li","doi":"10.1016/j.aqrep.2024.102366","DOIUrl":"10.1016/j.aqrep.2024.102366","url":null,"abstract":"<div><div>This study aimed to investigate the effects of dietary phospholipids (PLs) on growth performance, antioxidant capacity, and lipid metabolism of juvenile Chinese sturgeon (<em>Acipenser sinensis</em>). Juvenile sturgeon, with an initial body weight of 12.27±0.03 g, were fed five diets containing graded levels of PLs (1.26 %, 3.29 %, 4.34 %, 5.93 %, and 7.02 %) in triplicate for 56 days. All experimental diets were formulated to be isonitrogenous (55 %) and isolipidic (10 %). Results indicated that specific growth rate increased from 2.21 to 2.89 with elevated levels of dietary PLs. The optimum PLs level for juvenile Chinese sturgeon was estimated to be 5.93 % of diet according to the broken-line regression analysis of the specific growth rate against dietary PLs levels. Diets containing higher levels of PLs (5.93 % and 7.02 % of diet) significantly reduced the lipid content of whole body and liver by around 30 % in juvenile sturgeon compared to the control group (<em>P</em> <0.05). The proportions of specific fatty acids present in the liver lipid were linearly correlated with their percentages in dietary lipid (<em>P</em> <0.05), whereas muscle fatty acid composition remained unaffected across different treatments (<em>P</em> >0.05). EPA and DHA were preferentially accumulated into the muscle tissue. Activities of digestive enzymes and antioxidant enzymes were linearly increased with incremental dietary PLs levels (<em>P</em> <0.001). Inclusion of PLs in diet significantly enhanced the expression of antioxidant genes with the transcription of <em>GSH-Px</em> in the liver of fish fed 7.02 % PLs of diet being approximately 8-fold greater than in those fish fed diet without PL addition (<em>P</em> <0.001). Dietary PLs significantly induced the gene expression related to lipid catabolism, and suppressed the transcription of genes involved in lipogenesis (<em>P</em> <0.001). Based on growth performance, antioxidant capacity, and liver biochemical parameters, inclusion of 5.93 % PLs (soy lecithin preparation) in the diets is recommend for juvenile Chinese sturgeon.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102366"},"PeriodicalIF":3.2,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S235251342400454X/pdfft?md5=8f188ce53c67209708065e6b11fe4cb1&pid=1-s2.0-S235251342400454X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142315980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-24DOI: 10.1016/j.aqrep.2024.102368
Xiaojun Yu , Zhenhua Wu , Yonghao Fu , Jinshu Guo , Kai Luo , Wanyou Zhou , Kangsen Mai , Wenbing Zhang
Five isonitrogenous and isolipidic experimental diets were formulated to investigate the influence of soy protein concentrate (SPC) replacing dietary fish meal (FM) on the growth, PI3K/AKT/TOR pathway, immunity of abalone Haliotis discus hannai and its resistance to Vibrio parahaemolyticus. The basal diet was designed with 20 % of FM. Based on it, the FM was replaced by SPC at 0 (CON), 25 % (SPC25), 50 % (SPC50), 75 % (SPC75) and 100 % (SPC100), respectively. After the 110-day feeding trial, it was shown that completely replacing FM with SPC had no adverse effects on growth, feed utilization and body composition of abalone. The highest weight gain rate of abalone was 119.52 %, which was observed in the SPC25 group. The trypsin activity in the digestive gland within the SPC25 and SPC50 groups exhibited a significant increase compared to that in the remaining groups. Relative to the CON group, the SPC100 group showed a significant reduction in total antioxidant capacity and an increase in malondialdehyde content within the cell-free hemolymph (CFH). The myd88 mRNA expression in the SPC25-SPC75 groups was significantly decreased compared with other groups. The highest expressions of pi3k, mtor and s6k, and the lowest expression of 4e-bp were found in the SPC25 group. The hardness of muscle was significantly increased with the increasing dietary SPC levels. The CON, SPC25, and SPC50 groups exhibited significantly lower cumulative mortality rates among abalones following the V. parahaemolyticus challenge. In conclusion, in terms of growth, FM can be totally replaced by SPC. Furthermore, replacing 25 % of dietary FM with SPC led to a significant enhancement in abalone growth. Moderately replacing FM with SPC (up to 50 %) could improve abalone digestive capacity and did not significantly affect abalone survival following a V. parahaemolyticus challenge. The optimal replacement level of FM by SPC (≤ 25 %) triggered the PI3K/AKT/TOR pathway to promote protein synthesis. Considering the growth, digestion, immunity, muscle quality and disease resistance of abalone, it is recommended that dietary FM can be replaced by SPC not more than 50 %.
{"title":"Replacement of dietary fish meal with soy protein concentrate on the growth performance, PI3K/AKT/TOR pathway, immunity of abalone Haliotis discus hannai and its resistance to Vibrio parahaemolyticus","authors":"Xiaojun Yu , Zhenhua Wu , Yonghao Fu , Jinshu Guo , Kai Luo , Wanyou Zhou , Kangsen Mai , Wenbing Zhang","doi":"10.1016/j.aqrep.2024.102368","DOIUrl":"10.1016/j.aqrep.2024.102368","url":null,"abstract":"<div><div>Five isonitrogenous and isolipidic experimental diets were formulated to investigate the influence of soy protein concentrate (SPC) replacing dietary fish meal (FM) on the growth, PI3K/AKT/TOR pathway, immunity of abalone <em>Haliotis discus hannai</em> and its resistance to <em>Vibrio parahaemolyticus</em>. The basal diet was designed with 20 % of FM. Based on it, the FM was replaced by SPC at 0 (CON), 25 % (SPC25), 50 % (SPC50), 75 % (SPC75) and 100 % (SPC100), respectively. After the 110-day feeding trial, it was shown that completely replacing FM with SPC had no adverse effects on growth, feed utilization and body composition of abalone. The highest weight gain rate of abalone was 119.52 %, which was observed in the SPC25 group. The trypsin activity in the digestive gland within the SPC25 and SPC50 groups exhibited a significant increase compared to that in the remaining groups. Relative to the CON group, the SPC100 group showed a significant reduction in total antioxidant capacity and an increase in malondialdehyde content within the cell-free hemolymph (CFH). The <em>myd88</em> mRNA expression in the SPC25-SPC75 groups was significantly decreased compared with other groups. The highest expressions of <em>pi3k</em>, <em>mtor</em> and <em>s6k</em>, and the lowest expression of <em>4e-bp</em> were found in the SPC25 group. The hardness of muscle was significantly increased with the increasing dietary SPC levels. The CON, SPC25, and SPC50 groups exhibited significantly lower cumulative mortality rates among abalones following the <em>V. parahaemolyticus</em> challenge. In conclusion, in terms of growth, FM can be totally replaced by SPC. Furthermore, replacing 25 % of dietary FM with SPC led to a significant enhancement in abalone growth. Moderately replacing FM with SPC (up to 50 %) could improve abalone digestive capacity and did not significantly affect abalone survival following a <em>V. parahaemolyticus</em> challenge. The optimal replacement level of FM by SPC (≤ 25 %) triggered the PI3K/AKT/TOR pathway to promote protein synthesis. Considering the growth, digestion, immunity, muscle quality and disease resistance of abalone, it is recommended that dietary FM can be replaced by SPC not more than 50 %.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102368"},"PeriodicalIF":3.2,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352513424004563/pdfft?md5=a386e419bc4df07b175799fee4b7c642&pid=1-s2.0-S2352513424004563-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142315981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-24DOI: 10.1016/j.aqrep.2024.102377
Minxin Liang , Wenhao Yu , Kaiyao He , Qingheng Wang , Yongshan Liao , Ronglian Huang , Dewei Cheng , Yuewen Deng , Zhe Zheng , Chuangye Yang
The metamorphosis of the planktonic larvae of bivalve species plays a key role in their complex life history strategies, and this process involves changes in the molecular machinery required for sessile life and the development of intricate shells. Understanding the key molecular agents and special requirements in this development stage is critically important for increasing the production of larvae and promoting population recovery. Here, we cultured larvae of the giant pearl oyster Pinctada maxima, a protected bivalve species in China that is used for the commercial production of large pearls. RNA sequencing was used to identify differentially expressed genes (DEGs) before (E) and after metamorphosis (ES). A total of 8270 DEGs were identified. The up-regulation of genes related to oxidative phosphorylation at the ES stage indicate an increase in energy metabolism. The DEGs were systematically determined by conserved domain analysis. DEGs involved in apoptosis and autophagy coincided with changes in morphology and tissue reconstruction at the metamorphosis stage. In addition, the increased expression of genes related to hormone, neurotransmitter/neuropeptide receptor, and immunitye at the ES stage suggests that they play an important role in the induction of various metabolic processes and the development of the immune system following exposure to new environments. The high expression of vitamin B transporters indicates that the absorption and bioavailability of vitamin B are particularly important for the metamorphosis of P. maxima. These findings provide new insights into the factors inducing metamorphosis and the nutritional requirements of rare bivalve species, which have implications for the population restoration and breeding of bivalves.
双壳类浮游幼体的变态在其复杂的生活史策略中起着关键作用，这一过程涉及无柄生活和复杂外壳发育所需的分子机制的变化。了解这一发育阶段的关键分子机制和特殊要求，对于提高幼虫产量和促进种群恢复至关重要。在这里，我们培养了巨型珍珠贝（Pinctada maxima）的幼虫，巨型珍珠贝是中国受保护的双壳贝类，用于商业化生产大型珍珠。我们使用 RNA 测序来鉴定变态前（E）和变态后（ES）的差异表达基因（DEGs）。共鉴定出 8270 个 DEGs。在 ES 阶段，与氧化磷酸化相关的基因上调，表明能量代谢增加。通过保守结构域分析系统地确定了 DEGs。涉及凋亡和自噬的 DEGs 与变态阶段形态和组织重建的变化相吻合。此外，与激素、神经递质/神经肽受体和免疫有关的基因在 ES 阶段的表达量增加，表明它们在暴露于新环境后诱导各种代谢过程和免疫系统的发育中发挥了重要作用。维生素B转运体的高表达表明，维生素B的吸收和生物利用率对于大菱鲆的变态过程尤为重要。这些发现为诱导双壳贝类变态的因素和稀有双壳贝类的营养需求提供了新的见解，对双壳贝类的种群恢复和繁殖具有重要意义。
{"title":"Transcriptome analysis provides novel insights into the factors influencing the settlement and metamorphosis of Pinctada maxima","authors":"Minxin Liang , Wenhao Yu , Kaiyao He , Qingheng Wang , Yongshan Liao , Ronglian Huang , Dewei Cheng , Yuewen Deng , Zhe Zheng , Chuangye Yang","doi":"10.1016/j.aqrep.2024.102377","DOIUrl":"10.1016/j.aqrep.2024.102377","url":null,"abstract":"<div><div>The metamorphosis of the planktonic larvae of bivalve species plays a key role in their complex life history strategies, and this process involves changes in the molecular machinery required for sessile life and the development of intricate shells. Understanding the key molecular agents and special requirements in this development stage is critically important for increasing the production of larvae and promoting population recovery. Here, we cultured larvae of the giant pearl oyster <em>Pinctada maxima</em>, a protected bivalve species in China that is used for the commercial production of large pearls. RNA sequencing was used to identify differentially expressed genes (DEGs) before (E) and after metamorphosis (ES). A total of 8270 DEGs were identified. The up-regulation of genes related to oxidative phosphorylation at the ES stage indicate an increase in energy metabolism. The DEGs were systematically determined by conserved domain analysis. DEGs involved in apoptosis and autophagy coincided with changes in morphology and tissue reconstruction at the metamorphosis stage. In addition, the increased expression of genes related to hormone, neurotransmitter/neuropeptide receptor, and immunitye at the ES stage suggests that they play an important role in the induction of various metabolic processes and the development of the immune system following exposure to new environments. The high expression of vitamin B transporters indicates that the absorption and bioavailability of vitamin B are particularly important for the metamorphosis of <em>P. maxima</em>. These findings provide new insights into the factors inducing metamorphosis and the nutritional requirements of rare bivalve species, which have implications for the population restoration and breeding of bivalves.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102377"},"PeriodicalIF":3.2,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352513424004654/pdfft?md5=d41e21cf09cb43c3ec4699edf1f7be98&pid=1-s2.0-S2352513424004654-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142315982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-24DOI: 10.1016/j.aqrep.2024.102389
Jia Xu , Fan Wang , Chaoqun Hu , Junxiang Lai , Shiwei Xie , Kefu Yu , Fajun Jiang
This study aimed to investigate the molecular mechanisms underlying the detrimental effects of high lipid (HL) and high plant-protein (HP) diets on gut health in pearl gentian grouper, Epinephelus fuscoguttatus♂ × Epinephelus lanceolatus♀. Thus, grouper were randomly distributed into 12 plastic tanks, which were randomly assigned to four diet groups: control (C, 9.48 % lipid, 46.21 % protein), high lipid (HL, 16.70 % lipid, 46.37 % protein), high plant-protein (HP, 9.38 % lipid, 46.50 % protein), high lipid and high plant-protein (HLP, 16.67 % lipid, 46.54 % protein). After an 8-week feeding trial, the HL and HP treatments led to impaired growth performance, compromised gut structure, suppressed immune components, reduced antioxidant defense through the Keap/Nrf2 signaling, and inhibited protein metabolism via the PI3K/AKT/mTOR signaling. Notably, their combined effects (HLP) further exacerbated it. A significant decrease in the proportion of unclassified_Peptostreptococcaceae, Clostridium, and Curvibacter in all treatment groups. The Streptococcus, Lactobacillus, and Bosea were influenced by the interaction between dietary lipids and plant proteins. 14 metabolites that exhibited significant differences among all treatment groups, including arginine, prostaglandin B1, cortexolone, alpha-linolenic acid, and allocholic acid. The HL treatment primarily affected pathways related to lipid metabolism and immunomodulation, while the HP treatment predominantly influenced pathways associated with protein metabolism and immunomodulation. Our findings also indicated that the HP factor played a more prominent role in shaping the metabolic response of fish to the HLP intervention. Furthermore, a significant positive correlation between the Clostridium and the metabolites Cortexolone and Allocholic acid, while a negative correlation was observed between the Streptococcus, Lactobacillus, and Lactococcus and these metabolites. In conclusion, our study demonstrates that excessive inclusion of plant proteins and/or lipids negatively affects gut health in grouper by disrupting oxidative stress, immune response, and protein metabolism through modulation of the gut microbial-metabolite crosstalk.
{"title":"Dietary high plant protein and high lipid impaired the intestine health of grouper by disrupting oxidative stress, immune response, and protein metabolism","authors":"Jia Xu , Fan Wang , Chaoqun Hu , Junxiang Lai , Shiwei Xie , Kefu Yu , Fajun Jiang","doi":"10.1016/j.aqrep.2024.102389","DOIUrl":"10.1016/j.aqrep.2024.102389","url":null,"abstract":"<div><div>This study aimed to investigate the molecular mechanisms underlying the detrimental effects of high lipid (HL) and high plant-protein (HP) diets on gut health in pearl gentian grouper, <em>Epinephelus fuscoguttatus</em>♂ × <em>Epinephelus lanceolatus</em>♀. Thus, grouper were randomly distributed into 12 plastic tanks, which were randomly assigned to four diet groups: control (C, 9.48 % lipid, 46.21 % protein), high lipid (HL, 16.70 % lipid, 46.37 % protein), high plant-protein (HP, 9.38 % lipid, 46.50 % protein), high lipid and high plant-protein (HLP, 16.67 % lipid, 46.54 % protein). After an 8-week feeding trial, the HL and HP treatments led to impaired growth performance, compromised gut structure, suppressed immune components, reduced antioxidant defense through the Keap/Nrf2 signaling, and inhibited protein metabolism via the PI3K/AKT/mTOR signaling. Notably, their combined effects (HLP) further exacerbated it. A significant decrease in the proportion of unclassified_<em>Peptostreptococcaceae</em>, <em>Clostridium</em>, and <em>Curvibacter</em> in all treatment groups. The <em>Streptococcus</em>, <em>Lactobacillus</em>, and <em>Bosea</em> were influenced by the interaction between dietary lipids and plant proteins. 14 metabolites that exhibited significant differences among all treatment groups, including arginine, prostaglandin B1, cortexolone, alpha-linolenic acid, and allocholic acid. The HL treatment primarily affected pathways related to lipid metabolism and immunomodulation, while the HP treatment predominantly influenced pathways associated with protein metabolism and immunomodulation. Our findings also indicated that the HP factor played a more prominent role in shaping the metabolic response of fish to the HLP intervention. Furthermore, a significant positive correlation between the <em>Clostridium</em> and the metabolites Cortexolone and Allocholic acid, while a negative correlation was observed between the <em>Streptococcus</em>, <em>Lactobacillus</em>, and <em>Lactococcus</em> and these metabolites. In conclusion, our study demonstrates that excessive inclusion of plant proteins and/or lipids negatively affects gut health in grouper by disrupting oxidative stress, immune response, and protein metabolism through modulation of the gut microbial-metabolite crosstalk.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102389"},"PeriodicalIF":3.2,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352513424004770/pdfft?md5=f6205ef4eae579545cf6102d1b04789f&pid=1-s2.0-S2352513424004770-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142316254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-23DOI: 10.1016/j.aqrep.2024.102370
Huapeng Chen, Huan Liang, Haoran Yu, Shukui Sun, Da Zheng, Lei Wang, Xinxin Wang, Junlong Sun, Jian Luo, Feibiao Song
The scale of Plectropomus leopardus breeding is continually expanding, and this has necessitated constant changes to ensure that breeding conditions are optimized. Background color is an important factor affecting fish feeding. In this study, fish (10.58 ± 0.03 g) were cultured on five background colors (red, blue, black, white, and transparent), and the growth status and intestinal microbes of these fish over 56 days were analyzed. Growth performance and the expression of growth-related genes were optimized when the background color was white. The white group had higher AMS and LPS enzyme activities and lower T-SOD and T-AOC enzyme activities, indicating that the white background could effectively promote feeding and reduce oxidative stress in P. leopardus. Additionally, changes in background color altered the composition and function of the intestinal microbiota. The diversity and abundance of intestinal microbiota, including the abundance of beneficial bacteria, were highest in fish on a white background. The abundances of Firmicutes and Actinobacteriota and the abundance of Bacillus were higher in the White group than in the other groups, indicating that a white background promotes the health of juvenile P. leopardus. In conclusion, a white background can promote the growth performance of P. leopardus and increase the abundance of beneficial intestinal microbes. Generally, the results of our study could be used to optimize the culture of P. leopardus.
{"title":"Effect of background color on the growth performance, digestive enzyme activity, antioxidant capacity, and intestinal microbiota of juvenile Plectropomus leopardus","authors":"Huapeng Chen, Huan Liang, Haoran Yu, Shukui Sun, Da Zheng, Lei Wang, Xinxin Wang, Junlong Sun, Jian Luo, Feibiao Song","doi":"10.1016/j.aqrep.2024.102370","DOIUrl":"10.1016/j.aqrep.2024.102370","url":null,"abstract":"<div><div>The scale of <em>Plectropomus leopardus</em> breeding is continually expanding, and this has necessitated constant changes to ensure that breeding conditions are optimized. Background color is an important factor affecting fish feeding. In this study, fish (10.58 ± 0.03 g) were cultured on five background colors (red, blue, black, white, and transparent), and the growth status and intestinal microbes of these fish over 56 days were analyzed. Growth performance and the expression of growth-related genes were optimized when the background color was white. The white group had higher AMS and LPS enzyme activities and lower T-SOD and T-AOC enzyme activities, indicating that the white background could effectively promote feeding and reduce oxidative stress in <em>P. leopardus</em>. Additionally, changes in background color altered the composition and function of the intestinal microbiota. The diversity and abundance of intestinal microbiota, including the abundance of beneficial bacteria, were highest in fish on a white background. The abundances of Firmicutes and Actinobacteriota and the abundance of <em>Bacillus</em> were higher in the White group than in the other groups, indicating that a white background promotes the health of juvenile <em>P. leopardus</em>. In conclusion, a white background can promote the growth performance of <em>P. leopardus</em> and increase the abundance of beneficial intestinal microbes. Generally, the results of our study could be used to optimize the culture of <em>P. leopardus</em>.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102370"},"PeriodicalIF":3.2,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352513424004587/pdfft?md5=560041453b4566fcb3417ecfea3f6993&pid=1-s2.0-S2352513424004587-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142311199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-22DOI: 10.1016/j.aqrep.2024.102375
Yang Gu , Xiaozhao Shangguan , Yingrui Mao , Weizhong Li , Yijin Ren , Zhengxin Li , Huohong lv , Yihan Wu , Guiling Wang
Star3 is located in sex hormone synthesis signaling pathway associated with gonadal development. In this study, star3 was first cloned from the gonad of Hyriopsis cumingii. The cDNA of star3 in H. cumingii was 2072 bp in length, concluding an open reading frame (ORF) of 1419 bp, encoding 472 amino acids with a conserved START structural domain. Tissue distribution analysis showed star3 highest expressed mainly in gonad, which had higher transcription levels in testis than that in ovary. Significant hybridization signals were detected on the follicle wall cell of male gonad. Gonad expression patterns revealed star3 highest expressed at two months old during juvenile stages. After RNA interference with star3, expression levels of cyp17a and 17β-hsd11 was repressed. Furthermore, contents of pregnenolone, testosterone, and estradiol in gonad were reduced significantly. These results suggest star3 may be involved in sex hormone synthesis and play an important role in early gonadal development of H. cumingii.
{"title":"Molecular identification of a steroidogenesis-related gene, star3, and its function in gonadal development of Hyriopsis cumingii","authors":"Yang Gu , Xiaozhao Shangguan , Yingrui Mao , Weizhong Li , Yijin Ren , Zhengxin Li , Huohong lv , Yihan Wu , Guiling Wang","doi":"10.1016/j.aqrep.2024.102375","DOIUrl":"10.1016/j.aqrep.2024.102375","url":null,"abstract":"<div><div><em>Star3</em> is located in sex hormone synthesis signaling pathway associated with gonadal development. In this study, <em>star3</em> was first cloned from the gonad of <em>Hyriopsis cumingii</em>. The cDNA of <em>star3</em> in <em>H. cumingii</em> was 2072 bp in length, concluding an open reading frame (ORF) of 1419 bp, encoding 472 amino acids with a conserved START structural domain. Tissue distribution analysis showed <em>star3</em> highest expressed mainly in gonad, which had higher transcription levels in testis than that in ovary. Significant hybridization signals were detected on the follicle wall cell of male gonad. Gonad expression patterns revealed <em>star3</em> highest expressed at two months old during juvenile stages. After RNA interference with <em>star3</em>, expression levels of <em>cyp17a</em> and <em>17β-hsd11</em> was repressed. Furthermore, contents of pregnenolone, testosterone, and estradiol in gonad were reduced significantly. These results suggest <em>star3</em> may be involved in sex hormone synthesis and play an important role in early gonadal development of <em>H. cumingii</em>.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102375"},"PeriodicalIF":3.2,"publicationDate":"2024-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352513424004630/pdfft?md5=fb5f672508da0feae83abb63401efad6&pid=1-s2.0-S2352513424004630-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142311198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-21DOI: 10.1016/j.aqrep.2024.102359
Elisa Torres-Lagos , Carlos Henríquez-Castillo , Carlos Méndez , María C. Morales , Claudia B. Cárcamo , Paola Navarrete , Paulina Schmitt , Katherina Brokordt
Biofloc technology (BFT) has emerged as a sustainable method for prawn cultivation, particularly for regions with limited freshwater resources. The freshwater prawn Cryphiops caementarius exhibit improved survival and growth rates when cultivated using BFT compared to traditional clear water (CW) systems. Despite the importance of bacterial microbiota in organismal health and performance, our study represents the first comprehensive investigation into the impact of BFT and CW culture systems on the composition, dynamics, stability, and functional potential of bacterial communities in both the rearing environment and intestines of C. caementarius. Utilizing deep amplicon 16S rRNA sequencing, we assessed the diversity and relative abundances of bacterial microbiota in CW and BFT water systems and in the intestines of prawns cultured in these systems over an extended period. Results revealed higher host-environment interaction in BFT, and higher bacterial diversity in BFT water systems and prawn intestines. The environment and prawn intestines from BFT were enriched with beneficial bacteria, while CW counterparts harbored potentially pathogenic bacteria. Planctomycetota was the dominant phylum in BFT, while Proteobacteria predominated in CW. Functional profiling indicated efficient support for chemoheterotrophic bacteria in both systems, with greater nitrogen processing in CW but greater nitrite detoxification in BFT system. Cellulolysis was higher in the intestines of prawns from BFT, potentially enhancing nutrient availability. Prawn intestine microbiota exhibited high plasticity post-immune challenge, with notable colonization by potentially pathogenic bacteria (Aeromonas and Pseudomonas) at the expense of a decrease in beneficial bacteria, in both systems. However, prawn intestines from BFT also showed high colonization by beneficial bacteria such as Shewanella, with potential for improving immune response. Overall, our study sheds light on the complex interplay between water and intestinal microbiota in BFT and CW systems during C. caementarius cultivation, offering insights into the potential for harnessing beneficial microbiota to improve prawn health and performance, particularly through the BFT system.
{"title":"Biofloc culture system shapes the structure and function of environmental and intestinal bacterial communities in the river prawn Cryphiops caementarius","authors":"Elisa Torres-Lagos , Carlos Henríquez-Castillo , Carlos Méndez , María C. Morales , Claudia B. Cárcamo , Paola Navarrete , Paulina Schmitt , Katherina Brokordt","doi":"10.1016/j.aqrep.2024.102359","DOIUrl":"10.1016/j.aqrep.2024.102359","url":null,"abstract":"<div><p>Biofloc technology (BFT) has emerged as a sustainable method for prawn cultivation, particularly for regions with limited freshwater resources. The freshwater prawn <em>Cryphiops caementarius</em> exhibit improved survival and growth rates when cultivated using BFT compared to traditional clear water (CW) systems. Despite the importance of bacterial microbiota in organismal health and performance, our study represents the first comprehensive investigation into the impact of BFT and CW culture systems on the composition, dynamics, stability, and functional potential of bacterial communities in both the rearing environment and intestines of <em>C. caementarius</em>. Utilizing deep amplicon 16S rRNA sequencing, we assessed the diversity and relative abundances of bacterial microbiota in CW and BFT water systems and in the intestines of prawns cultured in these systems over an extended period. Results revealed higher host-environment interaction in BFT, and higher bacterial diversity in BFT water systems and prawn intestines. The environment and prawn intestines from BFT were enriched with beneficial bacteria, while CW counterparts harbored potentially pathogenic bacteria. Planctomycetota was the dominant phylum in BFT, while Proteobacteria predominated in CW. Functional profiling indicated efficient support for chemoheterotrophic bacteria in both systems, with greater nitrogen processing in CW but greater nitrite detoxification in BFT system. Cellulolysis was higher in the intestines of prawns from BFT, potentially enhancing nutrient availability. Prawn intestine microbiota exhibited high plasticity post-immune challenge, with notable colonization by potentially pathogenic bacteria (<em>Aeromonas</em> and <em>Pseudomonas</em>) at the expense of a decrease in beneficial bacteria, in both systems. However, prawn intestines from BFT also showed high colonization by beneficial bacteria such as <em>Shewanella</em>, with potential for improving immune response. Overall, our study sheds light on the complex interplay between water and intestinal microbiota in BFT and CW systems during <em>C. caementarius</em> cultivation, offering insights into the potential for harnessing beneficial microbiota to improve prawn health and performance, particularly through the BFT system.</p></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102359"},"PeriodicalIF":3.2,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352513424004472/pdfft?md5=ef9c4de6df41230f7e9c3bb5cad30487&pid=1-s2.0-S2352513424004472-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142271079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-20DOI: 10.1016/j.aqrep.2024.102358
Ahmad Fikri Abdullah , Hasfalina Che Man , Abdulsalam Mohammed , Murni Marlina Abd Karim , Suleiman Usman Yunusa , Nurshahida Azreen Binti Mohd Jais
Aquaculture plays a pivotal role in global food production, grappling with distinct hurdles in water quality, feeding operation, and disease control. Efficient management of these core aquaculture operations has been acknowledged as a fundamental measure, yet remains unattainable through traditional methodologies. The advent of the Internet of Things (IoT) has opened up transformative avenues for real-time aquaculture operations. IoT solutions have emerged as a potent toolset, facilitating prompt monitoring, data collection, analysis, and control within aquatic environments. Notwithstanding its remarkable advantages, the technology is not devoid of limitations and areas requiring advancement. This paper examines the diverse applications of IoT in aquaculture, encompassing water quality monitoring, feeding strategies, and intelligent health inspection. Aquaculture challenges like sensor corrosion, data fusion limits, environmental impacts on transmission, and more have been thoroughly discussed. It also highlights IoT's potential in aquaculture, focusing on sensor advancements, artificial intelligent (AI) integration, and increased productivity. Presenting the IoT-aquaculture trajectory, this paper highlights IoT's potential in aquaculture while stressing the need to balance benefits with challenges.
{"title":"Charting the aquaculture internet of things impact: Key applications, challenges, and future trend","authors":"Ahmad Fikri Abdullah , Hasfalina Che Man , Abdulsalam Mohammed , Murni Marlina Abd Karim , Suleiman Usman Yunusa , Nurshahida Azreen Binti Mohd Jais","doi":"10.1016/j.aqrep.2024.102358","DOIUrl":"10.1016/j.aqrep.2024.102358","url":null,"abstract":"<div><p>Aquaculture plays a pivotal role in global food production, grappling with distinct hurdles in water quality, feeding operation, and disease control. Efficient management of these core aquaculture operations has been acknowledged as a fundamental measure, yet remains unattainable through traditional methodologies. The advent of the Internet of Things (IoT) has opened up transformative avenues for real-time aquaculture operations. IoT solutions have emerged as a potent toolset, facilitating prompt monitoring, data collection, analysis, and control within aquatic environments. Notwithstanding its remarkable advantages, the technology is not devoid of limitations and areas requiring advancement. This paper examines the diverse applications of IoT in aquaculture, encompassing water quality monitoring, feeding strategies, and intelligent health inspection. Aquaculture challenges like sensor corrosion, data fusion limits, environmental impacts on transmission, and more have been thoroughly discussed. It also highlights IoT's potential in aquaculture, focusing on sensor advancements, artificial intelligent (AI) integration, and increased productivity. Presenting the IoT-aquaculture trajectory, this paper highlights IoT's potential in aquaculture while stressing the need to balance benefits with challenges.</p></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102358"},"PeriodicalIF":3.2,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352513424004460/pdfft?md5=a676b49d700676a1e63d4308168dd29f&pid=1-s2.0-S2352513424004460-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142271194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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