Aquaculture Reports最新文献

{"title":"Effects of the fishmeal freshness on growth performance, immune response, hepatopancreatic health and anti-stress capacity of giant freshwater prawn (Macrobrachium rosenbergii)","authors":"Anran Wang ,&nbsp;Jie Xu ,&nbsp;Xin Zhang ,&nbsp;Xiaohan Liu ,&nbsp;Min Zhang ,&nbsp;Chungui Huang ,&nbsp;Huangen Chen ,&nbsp;Shuyan Miao","doi":"10.1016/j.aqrep.2025.103281","DOIUrl":"10.1016/j.aqrep.2025.103281","url":null,"abstract":"<div><div>Fishmeal is one of the main protein sources in aquafeed, and its quality directly affects the growth and health of aquatic animals. This research assessed the influence of fishmeal freshness on the growth, immune function, hepatopancreatic health and stress resistance of giant freshwater prawn. Prawns (2.36 ± 0.01 g) were assigned to three experimental diets containing domestic fishmeal with different total volatile basic nitrogen (TVB-N), including DT55 (Dietary TVB-N = 55 mg/100 g), DT65 (Dietary TVB-N = 65 mg/100 g), and DT76 (Dietary TVB-N = 80 mg/100 g). After the experimental period, the DT65 and DT80 diets significantly impaired growth performance and increased the feed conversion ratio (FCR) (<em>P</em> &lt; 0.05). Additionally, activities of hemolymph alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were markedly increased in the DT65 and DT80 groups, while total protein (TP) and nitric oxide (NO) contents showed the opposite trend (<em>P</em> &lt; 0.05). Moreover, the hepatopancreatic antioxidant system was significantly suppressed in the DT65 and DT80 groups, as indicated by marked reductions in total antioxidant capacity (T-AOC) and activities of antioxidant enzymes (<em>P</em> &lt; 0.05). In line with these changes, the hepatopancreatic expression of the apoptosis-related genes <em>caspase 8</em>, <em>caspase 9</em>, and <em>bok</em> were significantly up-regulated in the DT65 and DT80 groups, while the mRNA levels of the anti-apoptotic <em>iap</em> was significantly decreased (<em>P</em> &lt; 0.05). Finally, high-temperature and ammonia nitrogen stress tests demonstrated a significant decline in the survival rate of prawns in the DT65 and DT80 groups. Overall, these findings underscore the critical importance of the ingredient freshness in formulated diets for prawn, recommending that dietary TVB-N level not exceed 65 mg/100 g.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"46 ","pages":"Article 103281"},"PeriodicalIF":3.7,"publicationDate":"2025-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145734228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary corn starch dose-dependently enhances intestinal and hepatic health in largemouth bass (Micropterus salmoides)","authors":"Godfred Boateng Agyenim ,&nbsp;Fred Bissih ,&nbsp;Derrick Asare ,&nbsp;Beiping Tan ,&nbsp;Shiwei Xie ,&nbsp;Junming Deng","doi":"10.1016/j.aqrep.2025.103298","DOIUrl":"10.1016/j.aqrep.2025.103298","url":null,"abstract":"<div><div>The study aimed to investigate starch-induced hepatobiliary syndrome in juvenile largemouth bass. Six isonitrogenous diets (Con, CoS20, CoS40, CoS60, CoS80, CoS100) with corn starch substituting 0 %, 20 %, 40 %, 60 %, 80 %, and 100 % of wheat starch, respectively, were tested over 70 days. Three replicates per group and 30 fish per net, totaling 540 fish with an initial weight of 10.48 ± 0.05 g, were used. The results indicated a significant difference (<em>P</em> &lt; 0.05) in growth with optimal at 41–59 % corn starch inclusion. Fish fed CoS20-CoS80 showed improved postprandial insulin and glucose regulation, a sustained glycemic response, and enhanced glycolytic activity compared to the fish fed the Con diet (<em>P</em> &lt; 0.05). Gut microbiota analysis revealed that Con-fed fish had reduced Firmicutes-to-Bacteroidetes ratios and increased Proteobacteria/Fusobacteria, indicating dysbiosis, while CoS60 promoted Firmicutes dominance for efficient carbohydrate fermentation and energy extraction. Liver antioxidant indices and serum biomarkers revealed that both Con and CoS100 induced metabolic stress and hepatic damage, while CoS40-CoS80 significantly improved them (<em>P</em> &lt; 0.05). Histology confirmed that Con-fed fish developed lipid accumulation, endothelial thickening, and inflammation, whereas CoS60 maintained liver health. Gene expression analysis confirmed a profound health benefit within the CoS40-CoS80 range, characterized by a significant down-regulated pro-inflammatory cytokine and NF-κB Pathway genes (<em>P</em> &lt; 0.05), and up-regulated Interleukin-10 (IL-10) and gut tight Junction integrity genes (<em>P</em> &lt; 0.05). Concurrently, the CoS40-CoS80 diets significantly promoted (<em>P</em> &lt; 0.05) hepatic lipid catabolism (up-regulation of peroxisome proliferator-activated receptor alpha (PPARα), Carnitine O-palmitoyltransferase 1 (CPT1), and adipose triglyceride lipase (ATGL)) and export (up-regulation of Microsomal triglyceride transfer protein (MTP)), while reducing cholesterol synthesis (down-regulation of 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGCR)). The CoS40-CoS80 range optimally balanced metabolic efficiency, microbiota composition, and hepatic function while reducing oxidative stress. These findings demonstrate that partial (41–89 %) corn starch substitution for wheat starch improves metabolic homeostasis and health outcomes in largemouth bass aquaculture.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"46 ","pages":"Article 103298"},"PeriodicalIF":3.7,"publicationDate":"2025-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145734231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chicken soluble hydrolysate, an excellent alternative to fishmeal in the diets of Litopenaeus vannamei","authors":"Xiang Si ,&nbsp;Chunfeng Yao ,&nbsp;Rouxin Wu ,&nbsp;Wanzhen Ma ,&nbsp;Yidan Sun ,&nbsp;Junming Deng ,&nbsp;Beiping Tan ,&nbsp;Haijun Liang ,&nbsp;Shuyan Chi","doi":"10.1016/j.aqrep.2025.103295","DOIUrl":"10.1016/j.aqrep.2025.103295","url":null,"abstract":"<div><div>This experiment evaluated the use of chicken soluble hydrolysate as a substitute for fishmeal in the diet of <em>Litopenaeus vannamei</em>, providing new ideas for the development and utilization of animal protein sources in aquaculture feed. On the basis of the control group (FM) feed, fishmeal was replaced by 5 % by mass with the addition of 5 % chicken soluble hydrolysate 1 (CSH1), 2 (CSH2), 3 (CSH3), and 4 (CSH4), to prepare five experimental feeds with isonitrogenous and isolipidic. These feeds were used to feed white shrimp (initial average weight of 0.23 ± 0.00 g) for 56 days. The results showed that the addition of CSH in the feed can effectively improve the growth performance of shrimp. The weight gain rate of the FM group was significantly lower than that of the other groups (p &lt; 0.05), and the final body weight, specific growth rate, protein efficiency ratio, and lipid efficiency ratio were significantly lower than those of the CSH1, CSH2, and CSH3 groups (p &lt; 0.05). The feed conversion ratio of the groups FM and CSH4 was significantly higher than that of the other groups (p &lt; 0.05). The mucosal fold height and intestinal α-amylase activity were significantly lower than the CSH2 group (p &lt; 0.05). The activities of ALT and AST in the alternative groups were significantly reduced compared to the FM group (p &lt; 0.05). The MDA content of the FM group was not significantly different compared to the CSH1, CSH3, and CSH4 groups (p &gt; 0.05), but was significantly higher than that of the CSH2 group (p &lt; 0.05). The mRNA expression levels of immune-related genes such as <em>relish</em>, <em>dorsal</em>, <em>cru</em>, <em>alf</em>, <em>lyz</em>, <em>akp</em>, and <em>acp</em> in the hepatopancreas of the alternative groups were significantly upregulated (p &lt; 0.05). The results indicated that dietary chicken soluble hydrolysate (CSH), particularly CSH2, significantly enhanced intestinal health, antioxidant capacity, and growth performance in <em>Litopenaeus vannamei.</em></div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"46 ","pages":"Article 103295"},"PeriodicalIF":3.7,"publicationDate":"2025-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145734226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Citric acid could strengthen the intestinal tight junction barrier and improve intestinal immune function in vivo and vitro experiments of hybrid grouper (Epinephelus fuscoguttatus ♀ × E. lanceolatus ♂)","authors":"Jiacheng Zhang ,&nbsp;Fred Bissih ,&nbsp;Beiping Tan ,&nbsp;Shuang Zhang ,&nbsp;Qihui Yang ,&nbsp;Xiaohui Dong ,&nbsp;Shuyan Chi","doi":"10.1016/j.aqrep.2025.103282","DOIUrl":"10.1016/j.aqrep.2025.103282","url":null,"abstract":"<div><div>In this experiment, the primary intestinal epithelial cells (IECs) inflammation model was constructed to investigate the intestinal injury mechanism under high level addition of plant-derived proteins in hybrid grouper (<em>Epinephelus fuscoguttatus ♀ × E. lanceolatus ♂</em>), and citric acid (CA) addition was used to investigate the protective effect in the primary IECs inflammation model. The vitro experiment was fed 30 fishes to three replicates of each group for 8 weeks. In the inflammation model of IECs, the addition of CA (1 mM) remarkably enhanced cell survival, and significantly reduced the secretion of inflammatory cytokines TNF-α and IL-1β (<em>P</em> &lt; 0.05). CA activated phosphorylation of S6K and 4EBP-1 in the mTOR pathway and promoted the protein expression of tight junction proteins (ZO-1, E-cadherin, occludin and claudin-1) (<em>P</em> &lt; 0.05). The supplementation of CA in the diet also could improve the expression of tight junction in intestine of hybrid grouper (<em>P</em> &lt; 0.05). CA attenuated the protein expression of inflammatory cytokines (IL-1β, IL-6 and TNF-α) in inflammation models by regulating ERK and NF-κB pathways (<em>P</em> &lt; 0.05). These findings suggest that citric acid enhances cell survival, strengthens the intestinal tight junction barrier and enhances intestinal immune function in the LPS-stimulated inflammatory model of IECs. Citric acid could serve as an effective means to counteract growth inhibition and reduced digestibility caused by high proportions of plant protein in fish feed.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"46 ","pages":"Article 103282"},"PeriodicalIF":3.7,"publicationDate":"2025-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145734229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-omics analysis on the effect of weaning on larval rearing in Chinese longsnout catfish","authors":"Hongyan Tao ,&nbsp;Yong Xie ,&nbsp;Mao Li ,&nbsp;Yuheng Song ,&nbsp;Chongjiang Hu ,&nbsp;Deshou Wang ,&nbsp;Shuqing Zheng","doi":"10.1016/j.aqrep.2025.103290","DOIUrl":"10.1016/j.aqrep.2025.103290","url":null,"abstract":"<div><div>Chinese longsnout catfish (<em>Leiocassis longirostris</em>), endemic to the Yangtze River in China, faces germplasm depletion due to poor larval survival and growth during seedling cultivation, exacerbated by limited understanding of weaning at the molecular level. Here, the results of growth performance and digestive enzyme activity showed that live prey, such as <em>Limnodilus</em> spp. and <em>Artemia</em> nauplii was the most suitable food for first feeding. Notably, larvae fed <em>Limnodilus</em> spp. for more than five days could undergo sex reversal, opening up scientific avenues for the cultivation of YY supermale and all-male progeny production. For weaning, a combined feeding of live prey and microbound diet for five days was found to be more conducive. RNA-seq analysis of gastrointestinal tract (GIT) during weaning (pre-weaning, mid-weaning and post-weaning) identified 3728 differentially expressed genes enriched in protein digestion and absorption, cholesterol metabolism, starch and sucrose metabolism, and NF-kappa B signaling pathways. Furthermore, the expression patterns of appetite-regulating genes varied during weaning, with the appetite-suppressing gene <em>cck</em> prominently expressed during the mid-weaning and appetite-stimulating genes <em>npy</em> and <em>agrp</em> significantly upregulated during the post-weaning. 16S rRNA sequencing revealed the establishment of a stable core microbial community in the GIT during weaning, with increasing richness and diversity as the weaning progressed, dominated by Proteobacteria, Bacteroidota, and Firmicutes. In conclusion, this study contributed to improving early survival and growth performance of <em>L. longirostris</em> and provided valuable insights into the effects of weaning on body metabolism and intestinal microbial community in farmed carnivorous fish.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"46 ","pages":"Article 103290"},"PeriodicalIF":3.7,"publicationDate":"2025-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145734227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular and enzymatic characterization of MMP19 in cuttlefish Sepia pharaonis: Insights into its role in Vibrio-induced skin ulceration","authors":"Haijuan Dai ,&nbsp;Yueping Chen ,&nbsp;Yonghua Hu ,&nbsp;Qingxi Han ,&nbsp;Weiwei Zhang","doi":"10.1016/j.aqrep.2025.103276","DOIUrl":"10.1016/j.aqrep.2025.103276","url":null,"abstract":"<div><div>Matrix metalloproteinases (MMPs) play crucial roles in various biological processes, including wound healing, inflammation, and immune defense, with MMP19 being one of the key members. In this study, one <em>mmp19</em> gene from cuttlefish <em>Sepia pharaonis</em> was cloned. The open reading frame (ORF) of <em>mmp19</em> is 1404 bp in length, encoding a predicted protein of 467 amino acids with a calculated molecular weight of 52.73 kDa. The recombinant MMP19 (rMMP19) was expressed in <em>Escherichia coli</em> BL21(DE3) and purified using Ni-NTA affinity chromatography. The optimal temperature and pH for rMMP19 enzyme activity were approximately 40 °C and pH 9.0, respectively. Moreover, rMMP19 exhibited significant collagenase activity using the collagen of <em>S. pharaonis</em> as substrate. Real-time reverse transcriptase PCR result showed that the mRNA level of MMP19 in the digestive gland of <em>S. pharaonis</em> was significantly upregulated upon stimulation with pathogen <em>Vibrio alginolyticus</em> H1, whereas it was markedly downregulated when exposed to the ColA-deficient mutant strain (Δ<em>colA</em>). Furthermore, molecular docking and enzyme activity assays were combined to screen inhibitors of the collagenase activities of MMP19 and ColA. The results demonstrated that curcumin, palmatine hydrochloride, and riboflavin showed significantly inhibitory effects on the enzyme activities of both MMP19 and ColA. These findings suggest that the pathogen <em>V. alginolyticus</em> could upregulate the expression of host MMP19 that accelerates the onset and progression of skin ulceration syndrome in <em>S. pharaonis</em> by degrading skin collagen.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"46 ","pages":"Article 103276"},"PeriodicalIF":3.7,"publicationDate":"2025-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145734225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beyond genotyping: Using ddRADseq GBS for pathogen surveillance in aquaculture – A case study in Barramundi (Lates calcarifer)","authors":"Celestine Terence ,&nbsp;Zhi Weng Josiah Poon ,&nbsp;Saengchan Senapin ,&nbsp;Ha Thanh Dong ,&nbsp;Shannon M. Clarke ,&nbsp;Rudiger Brauning ,&nbsp;Ken G. Dodds ,&nbsp;Nguyen Thanh Vu ,&nbsp;Xueyan Shen ,&nbsp;Susan Gibson-Kueh ,&nbsp;Dean R. Jerry ,&nbsp;Jose A. Domingos","doi":"10.1016/j.aqrep.2025.103273","DOIUrl":"10.1016/j.aqrep.2025.103273","url":null,"abstract":"<div><div>Genotyping by sequencing (GBS) is widely employed in aquaculture selective breeding programs to acquire SNP genotypes for various applications, including pedigree reconstruction, GWAS, and in the estimation of genetic parameters, genomic relationships and breeding values. Because sequencing libraries encompass all DNA present in fin-clip tissue, they also recover the non-host metagenomic fraction, including pathogens. We leveraged this property to survey for the presence of scale-drop disease virus (SDDV), responsible for 40–90 % mortality in farmed barramundi, while simultaneously genotyping the host. Raw reads from 4484 fish of four commercial cohorts (2239 moribund, 2245 asymptomatic) were aligned to the SDDV reference genome, and viral reads were normalised as reads per million (RPM) per individual. SDDV prevalence and load were tightly associated with clinical status: 88.9 % of moribund fish carried SDDV at 21.8 ± 0.6 RPM, whereas only 0.2 % of healthy fish were positive with 0.002 ± 0.001 RPM. Independent validation by quantitative PCR on fin and spleen from a nested subset of 172 fish (81 moribund, 91 healthy) yielded viral copy numbers strongly correlated with ddRADseq RPM (Spearman’s ρ = 0.84 for fin; ρ = 0.76 for spleen; both P &lt; 0.0001). Viral load was consistently higher in fin (mean 281 ± 45 copies ng⁻¹ DNA) than spleen (147 ± 32 copies ng⁻¹), corroborating the suitability of non-lethal fin tissue for surveillance. Prevalence and load distributions were homogeneous across cohorts, and no qPCR-positive individuals escaped detection by ddRADseq. These findings show that routine ddRADseq datasets in barramundi can also be repurposed into a sensitive epidemiological assay that unites SDDV monitoring with genomic improvement. Further, these findings suggest that breeding programs generating large ddRADseq GBS datasets may also serve pathogen surveillance purposes where the target pathogen infects the host genotyped tissue.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"46 ","pages":"Article 103273"},"PeriodicalIF":3.7,"publicationDate":"2025-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145734278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Implantation of aromatase inhibitor fadrozole and 17β-estradiol antagonistically affect gonad development in the protandrous barramundi (Lates calcarifer)","authors":"Quyen Q.T. Banh ,&nbsp;Jarrod L. Guppy ,&nbsp;Julian R. Wilson ,&nbsp;Jose A. Domingos ,&nbsp;Dean R. Jerry","doi":"10.1016/j.aqrep.2025.103261","DOIUrl":"10.1016/j.aqrep.2025.103261","url":null,"abstract":"<div><div>Barramundi, <em>Lates calcarifer</em>, are protandrous hermaphrodites, maturing and reproducing first as males before naturally changing sex into females several years later. This male-to-female development provides an excellent model for investigating the genetic mechanism underlying natural sex change in fish, and in aquaculture manipulating this process is key to control reproduction and enhance seedstock production. Exogenous hormones and aromatase inhibitors have been used to obtain control of sex in a range of fish. Fadrozole (FAD) is a non-steroidal inhibitor that reduces biosynthesis of estrogenic hormones, however its use has yet to be tested in barramundi. In this study, 15-month-old male barramundi (2102 ± 126 g) were divided into four treatments and either implanted with i) 17β-estradiol (E2) at 8 mg kg⁻¹, ii) FAD at 8 mg kg⁻¹, iii) 8 mg E2 and 8 mg FAD kg⁻¹ together, or iv) implanted with cholesterol pellets (controls). After nine weeks, gonadal phenotype was examined histologically and the expression of sex-related genes were quantified by qPCR. All fish with E2 only implants had sex-changed into females, with significantly higher <em>cyp19a1a</em> and <em>foxl2</em> expression and lower <em>dmrt1</em>, <em>cyp11b</em> and <em>esr1</em> expression than control fish. All fish treated with FAD only remained male, and expression of male genes (<em>dmrt1</em> and <em>cyp11b</em>) were significantly upregulated, whilst the female-biased gene foxl2 was downregulated. Combined FAD and E2 treatment resulted in 42 % of fish remaining male, 42 % becoming female and 16 % that were transitional, with each individuals gene expression patterns reflecting their gonadal phenotype. These results show FAD can impede the feminizing effects of E2 in some individuals, indicating potential for holding barramundi as male for longer before they sex-change. These findings highlight utility of E2 and FAD as tools for modulating sex change in barramundi and inform strategies for improved reproductive control in aquaculture breeding programs.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"46 ","pages":"Article 103261"},"PeriodicalIF":3.7,"publicationDate":"2025-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145734224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biological characterization and genome analysis of pathogenic Yersinia ruckeri causing enteric redmouth disease (ERM) in crucian carp (Carassius carassius)","authors":"Pengfei Liao ,&nbsp;Qi Xu ,&nbsp;Zhihui Xiao ,&nbsp;Yingjuan Xie ,&nbsp;Fa Dai ,&nbsp;Jian Ye ,&nbsp;Weifen Li ,&nbsp;Chaonan Zhang ,&nbsp;Xiaodong Zhang","doi":"10.1016/j.aqrep.2025.103277","DOIUrl":"10.1016/j.aqrep.2025.103277","url":null,"abstract":"<div><div>Enteric redmouth disease (ERM) is a severe hemorrhagic bacterial infection that causes high mortality in kinds of fish. We isolated a dominant strain from diseased crucian carp, identified as <em>Yersinia ruckeri</em>, a member of Enterobacteriaceae, and confirmed this strain as the causative agent of the disease outbreak through morphological observation, histopathological examination, molecular diagnostic analyses and artificial infection. The results indicated that the isolated bacteria strain <em>Yersinia ruckeri</em> designated as QDH202303 (GenBank: CP177154) exhibited significant pathogenicity in crucian carp (<em>Carassius carassius</em>) and was the the primary cause of the disease outbreak. The LD50 value of <em>Y. ruckeri</em> was calculated as 3.3 × 10⁴ CFU/fish. In the antibiotic susceptibility assay, the bacterial strain QDH202303 was resistant to 32 common antibiotics except polymyxin B, which showed broad-spectrum antibiotic resistance. Through whole-genome sequencing and sequence analysis, 7 resistance genes, 697 virulence factors were identified, which were associated with pathogenicity and broad-spectrum drug resistance. What's more, 13 genomic islands were identified, and three of them were related to the Type III secretion system, flagella, and <em>yhlBA</em> respectively. To the best of our knowledge, this is the first report of <em>Y. ruckeri</em> being identified as the causative agent of ERM disease in crucian carp. This study would provide valuable insights for the prevention and management of ERM diseases in aquaculture.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"46 ","pages":"Article 103277"},"PeriodicalIF":3.7,"publicationDate":"2025-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145734279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anguillid herpesvirus: Current knowledge and future perspectives","authors":"Jun-Qing Ge,&nbsp;Jin-Xian Yang,&nbsp;Qiang Chen","doi":"10.1016/j.aqrep.2025.103275","DOIUrl":"10.1016/j.aqrep.2025.103275","url":null,"abstract":"<div><div>Eels (<em>Anguilla spp.</em>) possess considerable economic value worldwide due to their favorable taste and nutritional benefits. However, the eel aquaculture industry is confronted with substantial challenges from viral diseases. Among these, Anguillid herpesvirus (AngHV) is identified as one of the most harmful and influential viruses, leading to high mortality rates in eel populations, thereby resulting in considerable losses for cultivated eel populations and contributing to the decline of wild eel stocks. The virus was first isolated in the 1980s, and despite extensive research efforts; however, to date, no commercial vaccines or antiviral agents have been developed. This review aims to summarize the advancements in AngHV research, encompassing advances in understanding its etiology, pathogenicity, diagnostic methodologies, preventive measures, control strategies, and prospective research directions. The objective is to deepen understanding of the virus and facilitate the development of effective strategies for the prevention and control management of AngHV infections in eel aquaculture.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"46 ","pages":"Article 103275"},"PeriodicalIF":3.7,"publicationDate":"2025-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145734280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}