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Extraction, characterization & applications of natural dyes 天然染料的提取、表征及应用
Pub Date : 2018-11-01 DOI: 10.21746/APS.2018.7.11.4
Bushra Khan, Ramya Sindhyan, Anushi Divan, S. Rathod
Artificial dyes, widely used in everyday items, come with side effects not known to the common man. They are toxic, harmful & mostly carcinogenic. There has been a worldwide increase in the demand of natural dyes due to its therapeutic properties & no known side effects. The current research aimed at extracting dyes of different colors from natural plant sources, carrying out its characteristic analysis & its applications. The dyes were tested for their anti-microbial ability and were found to be inhibitory to common organisms like S. typhi, C. diphtheriae, S. aureus, etc. Phytochemical tests were performed and they confirmed presence of several important metabolites like Phenols, Tannins, Terpenoids, and many more. The dyes were tested for anti-oxidant activity using the FRAP assay and antioxidant levels ranging from 0.62 to 2.00 were obtained. Preparations of cosmetics like lip balms, was carried out, and dyes were also tested as natural food coloring agents. The results indicated that the dyes caused inhibition of wide range of bacteria and proved to be good sources of anti-oxidants. They departed a natural color to food items and hence can replace artificial food colorings. Thus the use of natural dyes should be promoted, as they are cheap, biodegradable and feasible.
在日常用品中广泛使用的人造染料,其副作用是普通人所不知道的。它们是有毒的,有害的,大多数是致癌的。由于天然染料的治疗特性和无已知副作用,世界范围内对天然染料的需求不断增加。本研究旨在从天然植物中提取不同颜色的染料,进行其特性分析及其应用。对染料的抑菌能力进行了测试,发现对伤寒葡萄球菌、白喉葡萄球菌、金黄色葡萄球菌等常见生物有抑制作用。他们进行了植物化学测试,确认了几种重要的代谢物,如酚类、单宁、萜类等。用FRAP法测定染料的抗氧化活性,得到的抗氧化水平在0.62 ~ 2.00之间。唇膏等化妆品的制备也进行了测试,染料也作为天然食用色素进行了测试。结果表明,该染料对多种细菌有抑制作用,是抗氧化剂的良好来源。它们为食品赋予了天然的颜色,因此可以取代人工食用色素。因此,应推广使用天然染料,因为它们便宜、可生物降解且可行。
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引用次数: 1
Studies on the efficacy and therapeutic potential of sweet basil against E.coli 罗勒对大肠杆菌的功效及治疗潜力研究
Pub Date : 2018-10-31 DOI: 10.21746/APS.2017.01.000
Sumit Kumar Singh, S. Sarsaiya
Ocimum basilicum leaves were evaluated for their phytochemical as well as anti bacterial properities. Two different solventsc chloroform and water was used. Disc diffusion method was adopted for evaluation of antimicrobial activity of medicinal leaves. The result shows that sweet basil leaves are having good antibacterial activity and thus showing minimum inhibitory concentration zone of 22 mm against chloroform extract and 16 mm against aqueous extract.
对罗勒叶的植物化学和抗菌性能进行了评价。使用了两种不同的溶剂:氯仿和水。采用圆盘扩散法评价药材叶片的抑菌活性。结果表明,罗勒叶具有良好的抑菌活性,对氯仿提取物的最小抑菌浓度区为22 mm,对水提物的最小抑菌浓度区为16 mm。
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引用次数: 0
Betaine Aldehyde Dehydrogenase (BADH) gene and free amino acid analysis in Rhizophora mucronata Lam. from Thalassery region of Kerala, India. 甜菜碱醛脱氢酶(BADH)基因及游离氨基酸分析。来自印度喀拉拉邦的Thalassery地区。
Pub Date : 2018-10-06 DOI: 10.21746/APS.2018.7.10.1
Anusha Sreeshan, S. Meera, A. Augustine
Mangroves are salt tolerant plants inhabiting saline environment. Multiple factors contribute to their salt tolerance and we need multifaceted approach to reveal the mechanisms of salt tolerance in the plant. In the present study, leaves of the mangrove, Rhizophora mucronata grown in the presence and absence of salt were used, free amino acids and the expression of selected genes were analyzed. Chromatographic technique showed the accumulation of free amino acids like proline, glycine, aspartic acid, valine, leucine and glutamic acid in the presence of salt. RNA was isolated from the leaf sample and cDNA was synthesized. Gene specific primers were designed and standardized. Among the genes studied (P5CS, BADH, NHX1), Betaine Aldehyde dehydrogenase (BADH) gene was found to be expressed.
红树林是生长在盐碱环境中的耐盐植物。其耐盐性是由多种因素决定的,我们需要从多方面揭示植物耐盐机制。本研究以生长在有盐和无盐条件下的红树(Rhizophora mucronata)叶片为研究对象,分析了游离氨基酸和所选基因的表达。色谱分析表明,在盐存在的情况下,游离氨基酸如脯氨酸、甘氨酸、天冬氨酸、缬氨酸、亮氨酸和谷氨酸积累较多。从叶片样品中分离RNA,合成cDNA。设计并标准化基因特异性引物。在所研究的基因(P5CS、BADH、NHX1)中,发现有甜菜碱醛脱氢酶(BADH)基因表达。
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引用次数: 0
Comparative studies of phytochemical, antimicrobial and antioxidant studies between two Lamiaceae species of Ocimum tenuiflorum L. and Ocimum sanctum L. from the regions of Visakhapatnam, Andhra Pradesh, India. 印度安得拉邦维萨卡帕特南地区两种Lamiaceae植物Ocimum tenuflorum L.和Ocimum sanctum L.植物化学、抗菌和抗氧化的比较研究。
Pub Date : 2018-10-06 DOI: 10.21746/APS.2017.02.009
Madhuri Tulugu, B. Sitalaxmi, B. Sujatha, F. Sultana
Tulsi is one of the most popular plant in history of India. Present investigation is to compare the phytochemical, antimicrobial and antioxidant parameters of organic solvent extracts of hexane, chloroform and methanol. Qualitative phytochemical tests were used to detect the presence of alkaloids, tannins, saponins, flavonoids, glycosides and phenols, while two quantitative methods; Ferric Reducing Antioxidant Power (FRAP) and diphenyl -1, 2-picryl hydrazyl (DPPH) were used to determine the antioxidant potential. The antimicrobial activity was determined by Agar well diffusion method and was screened against seven micro organisms. The highest antioxidant activities were observed in the methanol extracts of each plant, while the hexane extract showed the least activity irrespective of the method used. The presence of active phytochemical substances with antioxidant activities may provide substantial basis for the use of these plants in ethnomedicine.
图尔丝是印度历史上最受欢迎的植物之一。本研究比较了己烷、氯仿和甲醇有机溶剂提取物的植物化学、抗菌和抗氧化参数。采用定性植物化学法检测生物碱、单宁、皂苷、黄酮类、苷类和酚类成分,采用两种定量方法;用铁还原抗氧化能力(FRAP)和二苯基- 1,2 -苦基酰肼(DPPH)测定其抗氧化能力。采用琼脂孔扩散法测定其抑菌活性,并对7种微生物进行筛选。甲醇提取物的抗氧化活性最高,而己烷提取物的抗氧化活性最低。具有抗氧化活性的活性植物化学物质的存在为这些植物在民族医药中的应用提供了坚实的基础。
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引用次数: 0
Foliar micromorphological character studies on Trichosanthes L. (Cucurbitaceae) from Terai & Duars, West Bengal, India. 印度西孟加拉邦Terai & Duars地区瓜科Trichosanthes L.叶片微形态特征研究。
Pub Date : 2018-10-06 DOI: 10.21746/APS.2018.7.10.2
R. Chowdhury, Anurag Chowdhury, M. Chowdhury
Trichosanthes is one of the most economically important genera of the family Cucurbitaceae. Studies on trichome micro-morphology and stomata were investigated for six species of Trichosanthes. All species has been recorded from the Terai and Duars of West Bengal. Range of variability in Trichosanthes species are quite variable, trichomes types including uni-multiseriate, macro-microform, conical, candelabra, capitated, glandular and non- glandular. Stomatal character shows variability (Paracytic, Actinocytic, Anomocytic and Diacytic). Trichomes and stomatal characters are utilised for phenetic studies which found to be important taxonomic tools to solve any disputes at least at or below species level. 
瓜蒌属是葫芦科最重要的经济属之一。对6种毛蚕的毛状体微观形态和气孔进行了研究。所有物种都记录在西孟加拉邦的Terai和Duars。毛虫种类的变异范围是相当多样的,毛体类型包括单多序列、大微形态、圆锥形、烛台状、头状、腺状和非腺状。气孔特征表现出多变性(泛胞型、放线细胞型、失整型和双胞型)。毛状体和气孔特征被用于表型研究,这被发现是解决至少在物种水平或低于物种水平的任何争议的重要分类工具。
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引用次数: 1
Studies on the effect of salinity on some aspergilli of usar and fertile soil to assess possible ecological specialization 盐碱度对盐碱地和肥沃土壤中某些曲霉菌影响的研究,以评估可能的生态专门化
Pub Date : 2018-09-07 DOI: 10.21746/APS.2017.05.005
Sunanda Soni, R. Singh
A comparative salt (Viz., Sodium Sulphate, Sodium Chloride, Magnesium Sulphate) tolerance tests conducted in relation to usar (=Alkaline Soil) and fertile soil Aspergilli , revealed that over all, usar isolates showed more growth and tolerance on increased salt concentrations than their fertile counterparts. Among the three salts put to test, sodium chloride has shown pronounced toxic or inhibitory effect on Aspergilli at higher concentrations. The maximum tolerance has been exhibited by Aspergillus fumigatus as it grew up to twenty four percent concentration, however none of the other Aspergilli including both usar and fertile type showed such type of sustainability. Of the salts tested, Magnesium sulphate seemed to be the salt of more utility to both types of isolates as exhibited by their growth at different concentration levels and further all the Aspergilli tested showed more physical growth at different concentrations of this salt in comparison to other two salts tested. Salinity also affected the sporulation of different Aspergilli . Visual observations of culture of different Aspergilli revealed that usar islotaes comparatively showed more tolerance even in sporulation. Usar isolates showed the alkaliphilic nature by showing more salt tolerance and can be recognized as Haloalkaliphiles as they have attained ecological specialization to quite an extent.
对usar(碱土)和肥沃土壤曲霉进行的盐(即硫酸钠、氯化钠、硫酸镁)耐受性比较试验表明,总体而言,usar菌株比肥沃土壤曲霉对增加的盐浓度表现出更强的生长和耐受性。在试验的三种盐中,氯化钠在较高的浓度下对曲霉菌表现出明显的毒性或抑制作用。烟曲霉在浓度达到24%时表现出最大的耐受性,而其他曲霉包括usar型和可育型都没有表现出这种耐受性。在测试的盐中,硫酸镁似乎对两种类型的分离物都更有用,因为它们在不同浓度水平下的生长表现出来,而且与其他两种盐相比,所有测试的曲霉菌在不同浓度的硫酸镁下都表现出更多的物理生长。盐度对不同曲霉的产孢量也有影响。对不同曲霉菌培养的目视观察表明,在产孢过程中,沙曲霉表现出较强的耐受性。Usar菌株表现出较强的耐盐性,具有一定程度的生态专门化,可认定为嗜盐碱菌。
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引用次数: 0
Isolation and optimization of catechins from Acacia catechu and its antioxidant and anti-ageing properties in vitro 儿茶中儿茶素的分离、优化及其抗氧化抗衰老性能
Pub Date : 2018-09-07 DOI: 10.21746/aps.2017.6.12.19
B. Tota, Y. DurgaPrasadV.K
Catechin is a polyphenol antioxidant plant metabolite which extracted from A. catechu, a hot water extract of red heartwood of Acacia catechu Willd. It is evident from the literature survey that the catechin is a biologically important polyphenolic compound. Aging is a complex biological phenomenon, an inevitable process. Numerous studies have reported that continuous exposure to reactive oxygen species can stimulate skin aging and that excessive accumulation of fat can cause an impaired skin barrier and tissue structure alterations. Thus, the maintenance of antioxidant homeostasis and the suppression of adipose accumulation are important strategies for skin anti-aging. In our study, a fairly simple, but a unique process had developed the highly purified actives, Catechin , and to prove the synergistic applications of highly purified catechin with other novel anti-oxidants notably Ascorbic acid. In vitro studies performed with these compounds exhibited in anti-elastase assay.
儿茶素是一种多酚类抗氧化植物代谢物,主要提取自儿茶红心材的热水提取物。从文献调查中可以明显看出,儿茶素是一种重要的生物学多酚化合物。衰老是一种复杂的生物现象,是一个不可避免的过程。大量研究报道,持续暴露于活性氧会刺激皮肤老化,脂肪的过度积累会导致皮肤屏障受损和组织结构改变。因此,维持抗氧化稳态和抑制脂肪积累是皮肤抗衰老的重要策略。在我们的研究中,一个相当简单,但独特的过程已经开发出高度纯化的活性物质,儿茶素,并证明了高度纯化的儿茶素与其他新型抗氧化剂,特别是抗坏血酸的协同应用。用这些化合物进行的体外研究显示在抗弹性酶测定中。
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引用次数: 0
Checklist of Akshara vidyalaya campus flora, Venkatachalam, Nellore, Andhra Pradesh, India. 印度安得拉邦内洛尔文卡塔查拉姆,Akshara vidyalaya校园植物清单。
Pub Date : 2018-09-07 DOI: 10.21746/aps.2018.7.9.4
M. K., Kumudavalli M.V., M. K.
The present study was aimed at determining the plant species richness of the Akshara Vidyalaya (AV) campus, Nellore District. For this, the species richness data was obtained by intensive surveys in the campus from 2015–2017. We recorded 247 species in 194 genera belonging to 69 families composed of 52 trees, 55 shrubs and 140 herbs, two species are parasites. Poaceae (20 genera 24 species), Fabaceae (15 genera 21 species) are the two species richness families and an inventory checklist of all the species recorded is provided. This study reveals that AV campus is diverse and rich in ornamental/alien flora.
本研究旨在确定内洛尔区Akshara Vidyalaya (AV)园区的植物物种丰富度。为此,在2015-2017年对校园进行了密集调查,获得了物种丰富度数据。共记录到69科194属247种,乔木52种,灌木55种,草本140种,其中寄生物2种。禾科(20属24种)和豆科(15属21种)是两个物种丰富的科,并提供了所有记录的物种清单。研究结果表明,AV校区具有丰富的观赏植物和外来植物群。
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引用次数: 0
Cymbidium macrorhizon (Orchidaceae): A new record for the Flora of Odisha, India. 标题大花蕙兰(兰科):印度奥里萨邦植物区系新记录。
Pub Date : 2018-08-31 DOI: 10.21746/APS.2018.7.9.2
K. Mandal, S. S. Khora, T. Kar
Cymbidium macrorhizon Lindl. is reported first time for Odisha from Koira forest of Bonai Forest Division. The correct botanical nomenclature, brief taxonomic description, with photo plate of the taxon is provided for easy identification.
大花蕙兰首次在奥里萨邦报道来自博奈森林司的Koira森林。提供了正确的植物命名法,简要的分类描述,并附有分类单元的照片板,以便于识别。
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引用次数: 3
Family Solanaceae: Taxonomy and modern trends 茄科:分类与现代趋势
Pub Date : 2018-08-31 DOI: 10.21746/APS.2018.7.9.1
M. M. Ganaie, Z. Reshi, V. Verma, V. Raja
Solanaceae belongs to that group of families, which are included in almost all angiosperm classifications indicating naturalness of the family. The family being one of the most evolutionarily successful and advanced taxa shows astonishing level of diversity reflected in the form of various types of adaptations. The high level of diversity within the family in addition to other unusual features have reflected in poor understanding of its phylogenetics. Fossil record of angiosperms particularly of Solanaceae) is very meager and relatively recent, so use of morphology and other conventional characters is not convincing in revealing true phylogeny of the family. This all has necessitated using alternative marker types in phylogenetic analysis of the family. Since advent of molecular biology molecular markers have been constantly refined to serve the purpose. Solanaceous species, such as tomato, potato, chilli pepper, tobacco, and petunia serve as model systems for the investigation of molecular and agronomic questions, and the family is the subject of intensive phylogenetic studies that are providing new insights into species boundaries and generic relationships. Results from recent morphological, molecular, and biosystematic research have shed new light on the systematic relationships in the family at virtually all taxonomic levels from subfamily to variety.
茄科属于这一科,几乎在所有被子植物分类中都包含这一科,表明这一科的自然性。这个科是进化最成功和最先进的分类群之一,表现出惊人的多样性,反映在各种适应的形式上。除了其他不寻常的特征外,该家族内部的高度多样性反映了对其系统发育的了解不足。被子植物(尤其是茄科)的化石记录非常贫乏且相对较近,因此使用形态学和其他常规特征来揭示该科的真实系统发育并不具有说服力。这一切都有必要使用替代标记类型在系统发育分析的家庭。自分子生物学出现以来,分子标记一直在不断完善,以达到这一目的。茄科植物,如番茄、马铃薯、辣椒、烟草和矮牵牛花,是研究分子和农艺问题的模型系统,该科是深入的系统发育研究的主题,为物种边界和属属关系提供了新的见解。最近形态学、分子和生物系统研究的结果揭示了从亚科到品种几乎所有分类水平上的系统关系。
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引用次数: 2
期刊
Annals of Plant Sciences
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