Archives of oral biology最新文献_第10页

Triggering mitotic catastrophe by podophyllotoxin induces apoptosis in oral squamous cell carcinoma 鬼臼毒素诱发有丝分裂突变诱导口腔鳞癌细胞凋亡。

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2025-09-15 DOI: 10.1016/j.archoralbio.2025.106396

Su-Jung Choi , Ji-Hoon Kim , Hyun-Ji Kim , Dong-Guk Park , Bohwan Jin , Won Woo Lee , Kyoung-Ok Hong , Sak Lee , Thantrira Porntaveetus , Jae-Jin Cho , Seong-Doo Hong , Sung-Dae Cho

Objective

This study investigated the relationship between mitotic catastrophe (MC) and apoptosis in oral squamous cell carcinoma (OSCC) using podophyllotoxin (PPT), a natural compound with antimitotic properties.

Design

We evaluated the concentration-dependent effects of PPT on cell proliferation (CCK-8 and soft agar assays) and morphology (transmission electron microscopy). Mechanistic insights were obtained by assessing DNA damage (western blotting), cell cycle progression (sub-G₁ analysis), and apoptosis-related protein activation in both 2D and 3D spheroid models of HSC-3 oral squamous carcinoma cells.

Results

PPT exerted pronounced inhibitory effects on cell proliferation and anchorage-independent growth accompanied by morphological indications of MC, such as enlarged multinucleated cells. DNA damage induced by PPT resulted in ataxia telangiectasia mutated kinase and checkpoint kinase 2 activation, leading to G₂/M arrest and cyclin B1upregulation. Importantly, PPT-induced MC was followed by apoptosis, as evidenced by an increased sub-G₁ population, Annexin V positivity, and caspase activation. Mitochondrial dysfunction, as indicated by altered membrane potential and enhanced Bax expression, underscored the apoptotic process. Caspase-2 activation emerged as a pivotal event, cleaving Bid and establishing a link between MC and the intrinsic apoptotic pathway. The effects were consistent across both 2D and 3D models, suggesting a robust therapeutic potential.

Conclusions

This study provides compelling evidence supporting the potential therapeutic significance of inducing MC-mediated apoptosis in OSCC. The results underscore the role of PPT and its derivatives, such as etoposide and teniposide, in targeting rapidly dividing cancer cells through interference with mitotic progression, offering insights into novel therapeutic strategies for oral cancer.

目的：利用具有抗有丝分裂特性的天然化合物鬼臼毒素（PPT）研究口腔鳞状细胞癌（OSCC）有丝分裂突变（MC）与细胞凋亡的关系。设计：我们评估了PPT对细胞增殖（CCK-8和软琼脂测定）和形态（透射电镜）的浓度依赖性影响。通过评估HSC-3口腔鳞癌细胞的2D和3D球体模型中的DNA损伤（western blotting）、细胞周期进展（亚g1分析）和凋亡相关蛋白激活，获得了机制见解。结果：PPT对细胞增殖和非锚定生长有明显的抑制作用，并伴有多核细胞增大等形态学表现。PPT诱导的DNA损伤导致共济失调毛细血管扩张突变激酶和检查点激酶2活化，导致G2/M阻滞和细胞周期蛋白b1上调。重要的是，ppt诱导的MC随后是细胞凋亡，亚g1群增加、Annexin V阳性和caspase激活证明了这一点。线粒体功能障碍，如膜电位改变和Bax表达增强所示，强调了凋亡过程。Caspase-2的激活是一个关键事件，它切断了Bid，并在MC和内在凋亡途径之间建立了联系。这种效果在2D和3D模型中都是一致的，这表明它具有强大的治疗潜力。结论：本研究提供了令人信服的证据，支持诱导mc介导的细胞凋亡在OSCC中的潜在治疗意义。这些结果强调了PPT及其衍生物，如依托泊苷和天尼泊苷，在通过干扰有丝分裂进程靶向快速分裂的癌细胞中的作用，为口腔癌的新治疗策略提供了见解。

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引用次数: 0

Protective effect of a novel antioxidant gel containing resveratrol and sodium fluoride on dentin erosion in the presence of acquired salivary pellicle: An in vitro study 含有白藜芦醇和氟化钠的新型抗氧化凝胶在获得性唾液膜存在下对牙本质侵蚀的保护作用：体外研究。

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2025-09-15 DOI: 10.1016/j.archoralbio.2025.106395

Loraine Perez Manzoli , Luan Júlio Ruiz da Silva , George Clay dos Santos Caracas , Kalinca Furtado de Oliveira , Walessa Alana Braganca Aragão , Rafael Rodrigues Lima , Milton Carlos Kuga , Cristiane de Melo Alencar

Objectives

This study aimed to evaluate the protective effect of experimental gels containing resveratrol, with or without 1.23 % sodium fluoride (NaF), and their interaction with the salivary pellicle on dentin erosion.

Design

Gels with 5 % or 10 % resveratrol (w/w) were prepared, with NaF added at 1.23 % (w/w). Total polyphenol content (TPC) and antioxidant capacity (ORAC) were determined. Human dentin specimens (n = 210) were allocated into seven groups (n = 30): negative control, NaF (1.23 %), 5 % resveratrol, 10 % resveratrol, 5 % resveratrol + NaF, 10 % resveratrol + NaF, and positive control (commercial anti-erosion toothpaste). Each group was divided according to the presence or absence of salivary pellicle (n = 15). After gel application, specimens were submitted to a 7-day erosive–abrasive cycling protocol. Outcomes included surface loss (3D CLM), collagen degradation after collagenase, calcium release (atomic absorption spectrometry), and tubule obliteration (CLM). Data were analyzed using two-way ANOVA and Tukey’s test (α = 0.05).

Results

The 10 % resveratrol gel had the highest TPC and ORAC (p = 0.03), followed by 10 % resveratrol + NaF (p = 0.013), whereas 5 % resveratrol showed lower values (p = 0.021). The salivary pellicle significantly reduced dSL-eroded, dColl, and CaR in all groups (p = 0.001). Combined formulations showed the greatest protection: G5 and G6 had lower dSL-eroded (p = 0.002, 0.004), dColl (p = 0.014, 0.021), and dSL-total (p = 0.007, 0.008), with G6 showing the lowest CaR (p = 0.023).

Conclusion

The combination of 10 % resveratrol and NaF effectively protected against dentin erosion, with salivary pellicle enhancing these effects.

目的：研究含白藜芦醇实验凝胶在含1.23 %氟化钠（NaF）和不含1.23 %氟化钠（NaF）的情况下对牙本质侵蚀的保护作用及其与唾液膜的相互作用。设计：制备白藜芦醇含量为5 %或10 % （w/w）的凝胶，NaF添加量为1.23 % （w/w）。测定了总多酚含量（TPC）和抗氧化能力（ORAC）。人类牙质标本(n = 210)被分配到7个组(n = 30):消极的控制,氟化钠(1.23 %),5 %白藜芦醇,10 %白藜芦醇,5 %白藜芦醇+ 氟化钠,10 %白藜芦醇+ 氟化钠,和积极的控制(商业冲牙膏)。根据有无唾液膜进行分组（n = 15）。凝胶应用后，将标本提交至7天的侵蚀-磨蚀循环方案。结果包括表面损失（3D CLM），胶原酶后胶原降解，钙释放（原子吸收光谱法）和小管闭塞（CLM）。数据分析采用双因素方差分析和Tukey检验（α = 0.05）。结果:10 %白藜芦醇凝胶最高TPC和氧自由基吸收(p = 0.03),其次是10 %白藜芦醇+ 氟化钠(p = 0.013),而5 %白藜芦醇显示较低的值(p = 0.021)。所有组的唾液膜均显著降低了dsl侵蚀、dColl和CaR （p = 0.001）。联合配方的保护效果最好，G5和G6的dcl侵蚀（p = 0.002,0.004）、dcol （p = 0.014,0.021）和dsl总（p = 0.007,0.008）均较低，其中G6的CaR最低（p = 0.023）。结论：10% %白藜芦醇与NaF联合使用对牙本质侵蚀有较好的保护作用，且唾液膜的作用增强。

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引用次数: 0

Endocannabinoid system in periodontitis: A systematic review and in silico analyses 内源性大麻素系统在牙周炎：系统回顾和计算机分析。

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2025-09-13 DOI: 10.1016/j.archoralbio.2025.106394

Lélio Fernando Ferreira Soares , Jovânia Alves Oliveira , Andressa Vilas Boas Nogueira , Carlos Rossa Júnior , Suzane Cristina Pigossi , James Deschner , Joni Augusto Cirelli

Objective

This systematic review with in silico investigation discusses the involvement of the endocannabinoid system (ECS), particularly CB1 and CB2 receptors (genes CNR1 and CNR2 respectively), in periodontal health and disease (PD).

Methods

PubMed/MEDLINE, Embase, Web of Science, Scopus, and The Cochrane Library databases were searched for studies on periodontitis and ECS published up to August 2024. The GSE16134 dataset was used for analyses of differential gene expression, correlation of ECS genes, evaluation of biomarkers and functional enrichment analysis.

Results

Nine studies met the inclusion criteria (three clinical and six preclinical studies). Clinical studies demonstrated that CNR2 gene expression was significantly reduced in periodontitis, while CNR1 showed minor changes. Animal studies with CB2 activation by different therapies increased receptor expression, reduced pro-inflammatory cytokines, and mitigated alveolar bone loss. CB1 activation also reduced inflammation and bone loss. Anandamide (AEA), an endogenous ligand of the cannabinoid receptors, exhibited anti-inflammatory effects, with endogenous levels decreasing after therapy. Bioinformatics analysis revealed that CNR1 expression in PD tissues was positively associated with genes involved in B-cell activation and humoral immune responses. In contrast, CNR2 expression showed strong correlations with genes related to immune regulation and extracellular matrix remodeling, suggesting distinct yet complementary roles for CB1 and CB2 in periodontal inflammation.

Conclusions

The ECS participates in periodontal inflammation, with CB2 activation emerging as a promising therapeutic target.

目的：本系统综述结合计算机研究探讨了内源性大麻素系统（ECS），特别是CB1和CB2受体（分别为CNR1和CNR2基因）在牙周健康和疾病（PD）中的作用。方法：检索PubMed/MEDLINE、Embase、Web of Science、Scopus和The Cochrane Library数据库，检索截至2024年8月发表的有关牙周炎和ECS的研究。GSE16134数据集用于差异基因表达分析、ECS基因相关性分析、生物标志物评估和功能富集分析。结果：9项研究符合纳入标准（3项临床研究和6项临床前研究）。临床研究表明，牙周炎患者CNR2基因表达显著降低，而CNR1基因表达变化较小。在动物实验中，通过不同的治疗方法激活CB2可以增加受体表达，减少促炎细胞因子，减轻牙槽骨丢失。CB1激活还能减少炎症和骨质流失。大麻素受体的内源性配体Anandamide （AEA）表现出抗炎作用，治疗后内源性水平降低。生物信息学分析显示，CNR1在PD组织中的表达与参与b细胞活化和体液免疫反应的基因呈正相关。相比之下，CNR2的表达与免疫调节和细胞外基质重塑相关的基因有很强的相关性，这表明CB1和CB2在牙周炎症中具有不同但互补的作用。结论：ECS参与牙周炎症，CB2激活成为一个有希望的治疗靶点。

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引用次数: 0

Comparative analysis of antibacterial and antibiofilm potential of chemically- and green laser-produced gold and silver nanoparticles against dental pathogens 化学和绿色激光制备的金、银纳米颗粒对口腔病原体的抗菌和抗生物膜潜能的比较分析。

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2025-09-13 DOI: 10.1016/j.archoralbio.2025.106393

Milica Nemoda , Marija Radovanović , Sanja Živković , Miloš Momčilović , Vladimir Rajić , Marko Bošković , Biljana Nikolić , Jelena Marinković

Objectives

This study compared antibacterial and antibiofilm effectiveness of chemically (chem_) and pulsed laser ablation-synthesized (laser_) gold (Au) and silver (Ag) nanoparticles (NPs), namely chem_AuNPs, chem_AgNPs, laser_AuNPs, and laser_AgNPs, against four Streptococcus species (S. mutans, S. mitis, S. sanguinis, and S. gordonii), essential for cariogenic biofilm development.

Design

Nanoparticles were characterized for size and stability using dynamic light scattering (DLS) and transmission electron microscopy (TEM). Their antibacterial and antibiofilm activities were tested in microdilution and crystal violet assays, respectively. Ex vivo evaluation of the nanoparticles antibiofilm activity was performed against a four-species biofilm on enamel discs by quantifying CFU. Scanning electron microscopy was used to visualize the reduction in biofilm mass on enamel discs treated with laser_Au and Ag nanoparticles.

Results

TEM showed nanoparticle sizes ranging 5.89–18.49 nm. DLS revealed the stability of NPs colloid solutions, with ζ-potential approximately −30 mV over 21 days. All NPs exhibited strong antibacterial activity, with MIC values of 56.9–120.6 μg mL-1 for chem_AuNPs and 1.9–30.8 μg mL-1 for all the rest. The evaluation of NPs potential in biofilm prevention showed the following trend in activity: laser_AuNPs>laser_AgNPs>chem_AuNPs>chem_AgNPs. AuNPs, regardless of synthesis method, were as effective as the positive control (chlorhexidine-mouthwash) against the multispecies biofilm settled on enamel discs, while AgNPs were nearly twice as efficient.

Conclusions

AuNPs and AgNPs could be alternatives for managing cariogenic bacteria both planktonic and within biofilms. Laser_AgNPs, being more efficient than commercial mouthwash, seem to be the most valuable candidate.

目的：比较化学（chem_）和脉冲激光烧蚀合成（laser_）金（Au）和银（Ag）纳米颗粒（NPs），即chem_AuNPs、chem_AgNPs、laser_AuNPs和laser_AgNPs对四种链球菌（变形链球菌、米氏链球菌、血源链球菌和戈多氏链球菌）的抗菌和抗生物膜效果。设计：利用动态光散射（DLS）和透射电子显微镜（TEM）表征纳米颗粒的尺寸和稳定性。分别用微稀释法和结晶紫法测定其抗菌活性和抗生物膜活性。通过定量CFU对牙釉质盘上的四种生物膜进行体外抗生物膜活性评价。采用扫描电镜观察激光金、银纳米粒子对牙釉质盘生物膜质量的影响。结果：透射电镜显示纳米颗粒尺寸为5.89 ~ 18.49 nm。DLS显示了NPs胶体溶液的稳定性，在21天内，ζ电位约为-30 mV。所有NPs均表现出较强的抑菌活性，其中chem_AuNPs的MIC值为56.9 ~ 120.6 μ mL-1，其余NPs的MIC值为1.9 ~ 30.8 μ mL-1。NPs在生物膜预防中的活性评价显示：laser_AuNPs>laser_AgNPs>chem_AuNPs>chem_AgNPs。无论采用何种合成方法，AuNPs与阳性对照（氯己定漱口水）对沉积在牙釉质盘上的多物种生物膜的效果相同，而AgNPs的效果几乎是前者的两倍。结论：AuNPs和AgNPs可以作为控制浮游生物和生物膜内致龋细菌的替代方法。Laser_AgNPs比商业漱口水更有效，似乎是最有价值的候选者。

{"title":"Comparative analysis of antibacterial and antibiofilm potential of chemically- and green laser-produced gold and silver nanoparticles against dental pathogens","authors":"Milica Nemoda , Marija Radovanović , Sanja Živković , Miloš Momčilović , Vladimir Rajić , Marko Bošković , Biljana Nikolić , Jelena Marinković","doi":"10.1016/j.archoralbio.2025.106393","DOIUrl":"10.1016/j.archoralbio.2025.106393","url":null,"abstract":"<div><h3>Objectives</h3><div>This study compared antibacterial and antibiofilm effectiveness of chemically (chem_) and pulsed laser ablation-synthesized (laser_) gold (Au) and silver (Ag) nanoparticles (NPs), namely chem_AuNPs, chem_AgNPs, laser_AuNPs, and laser_AgNPs, against four <em>Streptococcus</em> species (<em>S. mutans, S. mitis, S. sanguinis,</em> and <em>S. gordonii</em>), essential for cariogenic biofilm development.</div></div><div><h3>Design</h3><div>Nanoparticles were characterized for size and stability using dynamic light scattering (DLS) and transmission electron microscopy (TEM). Their antibacterial and antibiofilm activities were tested in microdilution and crystal violet assays, respectively. <em>Ex vivo</em> evaluation of the nanoparticles antibiofilm activity was performed against a four-species biofilm on enamel discs by quantifying CFU. Scanning electron microscopy was used to visualize the reduction in biofilm mass on enamel discs treated with laser_Au and Ag nanoparticles.</div></div><div><h3>Results</h3><div>TEM showed nanoparticle sizes ranging 5.89–18.49 nm. DLS revealed the stability of NPs colloid solutions, with ζ-potential approximately −30 mV over 21 days. All NPs exhibited strong antibacterial activity, with MIC values of 56.9–120.6 μg mL-1 for chem_AuNPs and 1.9–30.8 μg mL-1 for all the rest. The evaluation of NPs potential in biofilm prevention showed the following trend in activity: laser_AuNPs>laser_AgNPs>chem_AuNPs>chem_AgNPs. AuNPs, regardless of synthesis method, were as effective as the positive control (chlorhexidine-mouthwash) against the multispecies biofilm settled on enamel discs, while AgNPs were nearly twice as efficient.</div></div><div><h3>Conclusions</h3><div>AuNPs and AgNPs could be alternatives for managing cariogenic bacteria both planktonic and within biofilms. Laser_AgNPs, being more efficient than commercial mouthwash, seem to be the most valuable candidate.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"179 ","pages":"Article 106393"},"PeriodicalIF":2.1,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145103136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioactive caries-preventing effects of mineral ions released from surface pre-reacted glass-ionomer (S-PRG) filler on oral biofilm 表面预反应玻璃离子（S-PRG）填料释放的矿物离子对口腔生物膜的生物活性防龋作用

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2025-09-08 DOI: 10.1016/j.archoralbio.2025.106392

Kazuo Kato , Ryo Kutsuna , Yoshiaki Kawamura , Yoshihiro Shimazaki

Objective

The bioactive caries-preventing effects of mineral ions released from surface pre-reacted glass-ionomer (S-PRG) filler on oral biofilm were estimated by analyzing the depth-specific density of Streptococcus mutans and Streptococcus sanguinis.

Design

Ten participants wore in situ plaque-generating enamel slab devices on their upper molars to form biofilm for 5 days. Filtrates were prepared from prophylaxis paste slurries containing S-PRG filler, whereas paste without the filler served as the control. The devices were alternately immersed in sucrose solution three times and in the experimental or control filtrate twice each day. Two biofilm samples taken from each device were separated into 6–9 layered fractions (100 μm each) by serial sectioning. One sample was used for extraction of genomic DNA. The gene sequences encoding D-alanine:D-alanine ligases were amplified using universal and species-specific primers. Borate, aluminum, silicate, and strontium concentrations were determined using thicker sections of the other sample, correcting for biomass volume estimated by the area measurement of stained thinner sections.

Results

The ratios of S. mutans to S. sanguinis in the outer, middle, and inner layers were 0.0359, 0.0254 and 0.0157 for the experimental biofilm and 0.046, 0.0325 and 0.0255 for control biofilm, respectively. The ratio in the whole layer was significantly lower in the experimental biofilm than control biofilm. Strontium and aluminum concentrations in the experimental biofilm were 114- to 146-fold and 2.74- to 4.48-fold higher compared with control biofilm. respectively.

Conclusions

These results suggest that S-PRG filler has bioactive caries-preventing effects on oral biofilm.

目的通过分析变形链球菌和血链球菌的深度特异性密度，评价表面预反应玻璃离子（S-PRG）填充物释放的矿物离子对口腔生物膜的生物活性防龋作用。10名参与者在上颌磨牙上佩戴原位牙菌斑生成牙釉质板装置，形成生物膜5天。滤液由含有S-PRG填料的预防膏浆制备，而不含填料的膏浆作为对照。该装置交替浸泡在蔗糖溶液中三次，在实验或对照滤液中每天两次。从每个装置上取2个生物膜样品，通过连续切片将其分成6-9层状（每层100 μm）。其中一个样本用于提取基因组DNA。用通用引物和种特异性引物扩增d -丙氨酸连接酶的基因序列。硼酸盐、铝、硅酸盐和锶的浓度使用其他样品的较厚切片来测定，校正通过染色较薄切片的面积测量估计的生物量。结果实验生物膜外、中、内层变形链球菌与血链球菌的比值分别为0.0359、0.0254和0.0157，对照生物膜为0.046、0.0325和0.0255。实验生物膜的全层比例显著低于对照生物膜。实验生物膜中锶和铝的浓度分别是对照生物膜的114 ~ 146倍和2.74 ~ 4.48倍。分别。结论S-PRG填料对口腔生物膜具有生物活性的防龋作用。

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引用次数: 0

Selective bacterial proteolysis in periodontal disease: Molecular mechanisms and therapeutic implications, a narrative review 牙周病的选择性细菌蛋白水解：分子机制和治疗意义

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2025-09-05 DOI: 10.1016/j.archoralbio.2025.106391

Andrea Escalante-Herrera, Walter L. Siqueira

Background/Objective

Periodontal diseases are characterized by complex interactions between the immune system, oral bacteria, host tissues, and various external and internal factors. A hallmark of disease progression is the selective degradation of host proteins by bacterial proteases. This review explores the molecular mechanisms of bacterial proteolysis, with a focus on the selectivity toward host proteins essential for oral homeostasis.

Methods

An extensive electronic search was conducted using PubMed, Scopus, and Web of Science, covering the last 20 years. Search terms included “degradation,” “oral tissue,” “proteins,” “bacteria,” “periodontal disease,” “periodontitis,” “pathways,” and “host.” Boolean operators and filters were applied to refine results. Articles were evaluated based on titles, abstracts, and full texts. Additional references were identified through citation tracking. A narrative synthesis approach was used to integrate findings.

Results

Bacterial proteases exhibit precise substrate recognition, targeting specific structural, immune, and signaling proteins in the oral environment. The proteolytic profile and disease pathology are shaped by microbial community dynamics, including cooperative and competitive interactions. Host factors such as genetic diversity, local microenvironmental conditions, and immune responses further modulate protein degradation. Despite advancements, critical gaps remain, particularly regarding post-translational modifications and the determinants of proteolytic specificity.

Conclusions

Selective bacterial proteolysis plays a pivotal role in periodontal disease pathogenesis. Addressing current knowledge gaps through advanced proteomics, longitudinal studies, and imaging technologies is essential for developing targeted diagnostic and therapeutic strategies. An integrative approach is needed to enhance periodontal disease management and improve patient outcomes.

背景/目的牙周病的特点是免疫系统、口腔细菌、宿主组织和各种外部和内部因素之间复杂的相互作用。疾病进展的一个标志是细菌蛋白酶对宿主蛋白的选择性降解。这篇综述探讨了细菌蛋白水解的分子机制，重点是对口腔内稳态所必需的宿主蛋白的选择性。方法利用PubMed、Scopus、Web of Science等数据库进行近20年的文献检索。搜索词包括“降解”、“口腔组织”、“蛋白质”、“细菌”、“牙周病”、“牙周炎”、“途径”和“宿主”。使用布尔运算符和过滤器来优化结果。文章根据标题、摘要和全文进行评估。通过引文跟踪确定了其他参考文献。采用叙事综合方法整合研究结果。结果细菌蛋白酶具有精确的底物识别能力，针对口腔环境中的特定结构、免疫和信号蛋白。蛋白质水解特征和疾病病理是由微生物群落动态形成的，包括合作和竞争的相互作用。宿主因素如遗传多样性、局部微环境条件和免疫反应进一步调节蛋白质降解。尽管取得了进展，但关键的差距仍然存在，特别是在翻译后修饰和蛋白水解特异性的决定因素方面。结论细菌选择性蛋白水解在牙周病发病中起关键作用。通过先进的蛋白质组学、纵向研究和成像技术来解决目前的知识差距，对于制定有针对性的诊断和治疗策略至关重要。需要一种综合的方法来加强牙周病的管理和改善患者的预后。

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引用次数: 0

Probiotic supplementation attenuates dental pain and inhibits pain-induced cognitive impairment in male rats 补充益生菌可减轻雄性大鼠牙痛并抑制疼痛引起的认知障碍

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2025-08-29 DOI: 10.1016/j.archoralbio.2025.106382

Pegah Abazari-Bozhgani , Saeed Esmaeili-Mahani , Mehdi Abbasnejad , Maryam Raoof , Frank Lobbezoo

Objectives

The gut-brain axis has emerged as a promising avenue for understanding the bidirectional communication between the gut microbiota and the central nervous system. This study investigated the potential impact of probiotics, Lactobacillus acidophilus (LA-5), Lactobacillus paracasei (L. casei 431), and Bifidobacterium lactis (BB-12), as well as their combination, on dental pulp pain management and cognitive functions.

Design

Forty-eight male Wistar rats were randomly assigned to six experimental groups (n = 8). The probiotics (10⁹ CFU) were orally administered for 14 consecutive days. Capsaicin (100 µg) was used to induce inflammatory pulp nociception. The Morris water maze task was used to evaluate learning and memory performance. Levels of the interleukin-1 beta (IL-1β) and tumour necrosis factor-alpha (TNF-α) cytokines in the animals' trigeminal ganglion (TG) and the brain-derived neurotrophic factor (BDNF), neuropeptide Y (NPY), tropomyosin receptor kinase B (Trk-B) and Cyclooxygenase-2 (COX-2) genes in the animals' hippocampus were determined using western blotting and RT-PCR, respectively.

Results

Intradental application of capsaicin induced nociceptive behavior and increased IL-1β and TNF-α in the TG of rats. Probiotics could attenuate nociception and prevent IL-1β and TNF-α overexpression. Furthermore, pain induced cognitive impairments, decreased BDNF, NPY, and Trk-B and increased COX-2 gene expression in rat hippocampus, which were inhibited by probiotics supplementation.

Conclusions

Our findings suggest that probiotics may play a role in orofacial pain relief and cognitive enhancement in painful situations by modulating gut microbiota composition and influencing protein levels and gene expression in brain regions associated with pain and cognition.

目的肠脑轴已成为理解肠道微生物群和中枢神经系统之间双向交流的一个有希望的途径。本研究探讨了益生菌、嗜酸乳杆菌（LA-5）、副干酪乳杆菌（L. casei 431）和乳酸双歧杆菌（BB-12）及其组合对牙髓疼痛管理和认知功能的潜在影响。48只雄性Wistar大鼠随机分为6个实验组（n = 8）。益生菌（109 CFU）连续口服14天。辣椒素（100 µg）诱导炎症性牙髓痛觉。Morris水迷宫任务用于评估学习和记忆表现。采用western blotting和RT-PCR分别检测动物三叉神经节（TG）中白细胞介素-1β (IL-1β)和肿瘤坏死因子-α (TNF-α)细胞因子水平和海马中脑源性神经营养因子（BDNF）、神经肽Y （NPY）、原肌球蛋白受体激酶B （Trk-B）和环氧化酶-2 （COX-2）基因水平。结果传统应用辣椒素可诱导大鼠的伤害性行为，提高大鼠TG中IL-1β和TNF-α水平。益生菌可减轻痛觉，防止IL-1β和TNF-α过表达。此外，补充益生菌可以抑制疼痛引起的认知障碍，降低大鼠海马中BDNF、NPY和Trk-B以及增加COX-2基因表达。结论益生菌可能通过调节肠道菌群组成，影响疼痛和认知相关脑区蛋白水平和基因表达，在疼痛情境下起到缓解口面部疼痛和增强认知的作用。

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引用次数: 0

Effect of parecoxib and dexamethasone on the temporomandibular joint of orchiectomized rats: Morphological and immunological analysis 帕瑞昔布和地塞米松对去睾丸大鼠颞下颌关节的影响：形态学和免疫学分析

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2025-08-21 DOI: 10.1016/j.archoralbio.2025.106381

Victor Augusto Benedicto dos Santos , Francisco Carlos Groppo , José Roberto Garcia de Toledo , Michael Henrique Araújo Monteiro , Guilherme Elias Pessanha Henriques , Sidney Raimundo Figueroba

Objective

To evaluate the effects of parecoxib (a selective COX-2 inhibitor) and dexamethasone (a corticosteroid) on the temporomandibular joint (TMJ) of orchiectomized rats, a model of testosterone deficiency, through histological and immunological analyses.

Methods

Thirty-six rats were divided into six groups (n = 6). Sham groups received saline, parecoxib (0.3 mg/kg), or dexamethasone (0.1 mg/kg). ORX groups received the same treatments. TMJs were processed for histological staining (toluidine blue and picrosirius red) and analyzed by histomorphometry, measuring total cartilage thickness and its layers (fibrous, proliferative, mature, hypertrophic). Cytokines (IL-1α, IL-1β, IL-6, TNF-α) were quantified by ELISA. Data were analyzed using ANOVA-Welch and Kruskal-Wallis tests.

Results

ORX increased IL-1α, IL-1β, and TNF-α levels. IL-6 was reduced by dexamethasone. Dexamethasone also decreased cartilage thickness and accelerated its differentiation into subchondral bone. In contrast, parecoxib preserved cartilage thickness, especially in the fibrous and proliferative layers, and increased proteoglycan content. Both drugs reduced inflammatory markers, but with distinct structural effects.

Conclusions

Testosterone deficiency enhanced TMJ inflammation and impaired cartilage structure. While both dexamethasone and parecoxib modulated these effects, their actions differed: dexamethasone promoted cartilage-to-bone differentiation, potentially unfavorable long term, whereas parecoxib preserved cartilage integrity. These findings underscore hormonal influence and support selective anti-inflammatory strategies for TMJ preservation under androgen deficiency.

目的通过组织学和免疫学分析，评价帕雷昔布（一种选择性COX-2抑制剂）和地塞米松（一种皮质类固醇）对睾丸激素缺乏模型大鼠去睾丸后颞下颌关节（TMJ）的影响。方法36只大鼠随机分为6组（n = 6）。假手术组给予生理盐水、帕瑞昔布（0.3 mg/kg）或地塞米松（0.1 mg/kg）。ORX组接受相同的治疗。对TMJs进行组织学染色（甲苯胺蓝和小sirius红），并进行组织形态学分析，测量软骨总厚度及其层数（纤维状、增生、成熟、肥厚）。ELISA法检测细胞因子（IL-1α、IL-1β、IL-6、TNF-α）。数据分析采用ANOVA-Welch和Kruskal-Wallis检验。结果sorx升高IL-1α、IL-1β、TNF-α水平。地塞米松降低IL-6。地塞米松还能降低软骨厚度，加速软骨向软骨下骨的分化。相反，帕瑞昔布保留了软骨的厚度，尤其是纤维层和增生层，并增加了蛋白多糖的含量。两种药物都能降低炎症标志物，但具有明显的结构效应。结论睾酮缺乏可加重颞下颌关节炎症，损害软骨结构。虽然地塞米松和帕瑞昔布都调节了这些作用，但它们的作用不同：地塞米松促进了软骨向骨的分化，长期来看可能不利，而帕瑞昔布保持了软骨的完整性。这些发现强调了激素的影响，并支持在雄激素缺乏的情况下选择抗炎策略来保护TMJ。

{"title":"Effect of parecoxib and dexamethasone on the temporomandibular joint of orchiectomized rats: Morphological and immunological analysis","authors":"Victor Augusto Benedicto dos Santos , Francisco Carlos Groppo , José Roberto Garcia de Toledo , Michael Henrique Araújo Monteiro , Guilherme Elias Pessanha Henriques , Sidney Raimundo Figueroba","doi":"10.1016/j.archoralbio.2025.106381","DOIUrl":"10.1016/j.archoralbio.2025.106381","url":null,"abstract":"<div><h3>Objective</h3><div>To evaluate the effects of parecoxib (a selective COX-2 inhibitor) and dexamethasone (a corticosteroid) on the temporomandibular joint (TMJ) of orchiectomized rats, a model of testosterone deficiency, through histological and immunological analyses.</div></div><div><h3>Methods</h3><div>Thirty-six rats were divided into six groups (n = 6). Sham groups received saline, parecoxib (0.3 mg/kg), or dexamethasone (0.1 mg/kg). ORX groups received the same treatments. TMJs were processed for histological staining (toluidine blue and picrosirius red) and analyzed by histomorphometry, measuring total cartilage thickness and its layers (fibrous, proliferative, mature, hypertrophic). Cytokines (IL-1α, IL-1β, IL-6, TNF-α) were quantified by ELISA. Data were analyzed using ANOVA-Welch and Kruskal-Wallis tests.</div></div><div><h3>Results</h3><div>ORX increased IL-1α, IL-1β, and TNF-α levels. IL-6 was reduced by dexamethasone. Dexamethasone also decreased cartilage thickness and accelerated its differentiation into subchondral bone. In contrast, parecoxib preserved cartilage thickness, especially in the fibrous and proliferative layers, and increased proteoglycan content. Both drugs reduced inflammatory markers, but with distinct structural effects.</div></div><div><h3>Conclusions</h3><div>Testosterone deficiency enhanced TMJ inflammation and impaired cartilage structure. While both dexamethasone and parecoxib modulated these effects, their actions differed: dexamethasone promoted cartilage-to-bone differentiation, potentially unfavorable long term, whereas parecoxib preserved cartilage integrity. These findings underscore hormonal influence and support selective anti-inflammatory strategies for TMJ preservation under androgen deficiency.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"179 ","pages":"Article 106381"},"PeriodicalIF":2.1,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144891908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation and characterization of the extracellular matrix in salivary gland tumors through histochemistry and second harmonic generation 唾液腺肿瘤细胞外基质的组织化学和二次谐波评价与表征

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2025-08-19 DOI: 10.1016/j.archoralbio.2025.106380

Maria Clara Falcão Ribeiro-de-Assis , Luccas Lavareze , Antonio Thiago Pereira Campos , Sílvio Roberto Consonni , Arthur Antolini-Tavares , Érika Said Abu Egal , Albina Altemani , Fernanda Viviane Mariano

Objective

To describe extracellular matrix (ECM) components in salivary gland tumors with and without myoepithelial differentiation.

Design

Five cases of each tumor type were analyzed: pleomorphic adenoma, myoepithelioma, adenoid cystic carcinoma, myoepithelial carcinoma, epithelial-myoepithelial carcinoma, and salivary duct carcinoma. Histochemical stains identified collagen fibers, elastin, proteoglycans (PG), and glycosaminoglycans (GAG). Collagen was further examined by second harmonic generation (SHG) in three regions: tumor stroma, extratumoral stroma, and capsule. ImageJ was used to analyze area fraction, mean gray level, entropy, and contrast. Data underwent statistical analysis (p < 0.0001).

Results

ECM composition varied among salivary gland tumors. Pleomorphic adenoma showed low elastin and collagen, but PG and GAG were present in over 50 % of cases. Myoepithelioma showed variable collagen and elastin, while adenoid cystic carcinoma had predominant collagen. Myoepithelial carcinoma presented inconsistent elastin and low collagen. Epithelial-myoepithelial carcinoma and salivary duct carcinoma showed intense collagen staining. SHG revealed higher area fraction and gray level in the extratumoral region. Entropy was significantly higher outside the tumor. Malignant tumors with myoepithelial differentiation showed increased values in all SHG parameters compared to benign counterparts.

Conclusions

Benign tumors expressed more PG and GAG, whereas malignant ones had increased collagen with structural alterations. SHG analysis indicated lower collagen intensity within tumors and highlighted the role of ECM remodeling in malignancy, invasion, and metastasis.

目的探讨有无肌上皮分化的唾液腺肿瘤细胞外基质（ECM）成分。设计分析多形性腺瘤、肌上皮瘤、腺样囊性癌、肌上皮癌、上皮-肌上皮癌和唾液管癌各5例。组织化学染色鉴定出胶原纤维、弹性蛋白、蛋白聚糖（PG）和糖胺聚糖（GAG）。在肿瘤间质、瘤外间质和囊膜三个区域进一步用二次谐波生成（SHG）检测胶原蛋白。ImageJ用于分析面积分数、平均灰度、熵和对比度。数据进行统计学分析（p <； 0.0001）。结果唾液腺肿瘤中secm的组成存在差异。多形性腺瘤表现为低弹性蛋白和胶原蛋白，但PG和GAG在50%以上 %的病例中存在。肌上皮瘤以胶原蛋白和弹性蛋白为主，腺样囊性癌以胶原蛋白为主。肌上皮癌表现为弹性蛋白不一致和胶原蛋白含量低。上皮-肌上皮癌和唾液管癌呈强烈的胶原染色。SHG显示瘤外区域的面积分数和灰度值较高。肿瘤外熵值明显增高。与良性肿瘤相比，具有肌上皮分化的恶性肿瘤的所有SHG参数值均升高。结论良性肿瘤中PG和GAG表达较多，恶性肿瘤中胶原蛋白表达增多，结构改变。SHG分析显示肿瘤内胶原蛋白强度较低，并强调了ECM重塑在恶性、侵袭和转移中的作用。

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引用次数: 0

Synergistic promotion of biomimetic enamel remineralization by amelogenin, amelotin, and ameloblastin C-terminal peptide in vitro 仿釉原蛋白、仿釉素和成釉素c端肽体外协同促进仿生牙釉质再矿化

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2025-08-18 DOI: 10.1016/j.archoralbio.2025.106379

Xiu Zhong , Lu Yin , Peng Zhang , Shaozhen Ma , Xiaohua Ren , Kun Tian

Objective

This study aimed to investigate the influence of human amelogenin (AM), amelotin (AMTN), and ameloblastin c-terminal peptide (AMBN-C), both individually and in combination, on the in vitro remineralization of enamel.

Design

AM, AMTN, and AMBN-C were recombined and purified in vitro and formulated into 200 µg/ml chitosan (CS) protein gels. Nine different gel groups, including saline, CS, CS-AM, CS-AMTN, CS-AMBN-C, CS-AM-AMTN, CS-AM-AMBN-C, CS-AMTN-AMBN-C, and CS-AM-AMTN-AMBN-C, were applied to artificial carious tooth samples. These samples were incubated in artificial saliva for seven days, and the enamel surface's mineralization was assessed using SEM and XRD.

Results

CS-AM, CS-AMTN, and CS-AMBN-C stimulated the formation of scattered "dumbbell-like", perpendicular "bud-like", and aggregated “clump-like” hydroxyapatite (HA) crystals, respectively. CS-AM-AMTN, CS-AM-AMBN-C, and CS-AMTN-AMBN-C led to the formation of perpendicular "dumbbell-like" and "bud-like", parallel "clump-like" and "fine-rod-like", and vertical "clump-like" and "fine-rod-like" HA crystals, respectively. CS-AM-AMTN-AMBN-C induced the formation of optimally shaped and aligned HA crystals, characterized by "rod-like" crystal bundles of uniform diameter, perpendicular to the enamel surface.

Conclusions

AM functions as a mineralization template, promoting the formation of HA crystal bundles. AMBN-C influences the morphology of crystal bundles, while AMTN regulates their arrangement, ultimately facilitating a mineralized surface that resembles natural enamel.

目的探讨人成釉素（amelogenin， AM）、成釉素（AMTN）和成釉素c端肽（AMBN-C）单独或联合作用对体外牙釉质再矿化的影响。将DesignAM、AMTN和AMBN-C体外重组纯化，配制成200 µg/ml壳聚糖（CS）蛋白凝胶。将生理盐水、CS、CS- am、CS- amtn、CS- ambn - c、CS- am - amtn - c、CS- amtn - ambn - c、CS- am - amtn - ambn - c、CS- am - amtn - ambn - c、CS- am - amtn - ambn - c 9种不同凝胶组应用于人工龋齿样品。在人工唾液中培养7天，用扫描电镜（SEM）和x射线衍射（XRD）检测牙釉质表面的矿化程度。结果scs - am、CS-AMTN和CS-AMBN-C分别刺激形成分散的“哑铃状”、垂直的“芽状”和聚集的“团块状”羟基磷灰石（HA）晶体。CS-AM-AMTN、CS-AM-AMBN-C和CS-AMTN-AMBN-C分别形成垂直的“哑铃状”和“芽状”、平行的“团状”和“细棒状”、垂直的“团状”和“细棒状”HA晶体。CS-AM-AMTN-AMBN-C诱导形成最佳形状和排列的HA晶体，其特征是直径均匀的“棒状”晶体束，垂直于牙釉质表面。结论sam作为矿化模板，促进了HA晶体束的形成。AMBN-C影响晶体束的形态，而AMTN调节它们的排列，最终促进类似天然珐琅的矿化表面。

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引用次数: 0