Archives of oral biology最新文献_第10页

Fractionated irradiation induces radioresistant oral carcinoma cells with enhanced malignant phenotypes 分次照射诱导具有放射耐受性的口腔癌细胞，并增强其恶性表型

IF 3 4区医学 Q1 Medicine

Archives of oral biology

Pub Date : 2024-05-03 DOI: 10.1016/j.archoralbio.2024.105988

Yangfan Liu , Tongxu Sun , Jin Yang, Jingjing Luo, Hongmei Zhou

Objective

The fact that certain oral carcinoma patients experience radiotherapy failure implies that a more radioresistant and aggressive phenotype of surviving cancer cells potentially occurs during treatment. Our study aimed to establish radioresistant oral cancer cells through a fractionated irradiation protocol that mimics clinically relevant radiotherapy dosing strategies and to investigate all-round alterations in the malignant phenotype.

Methods

Radioresistant oral carcinoma cells were generated by exposing Cal27 and Detroit 562 cells to 60 Gy radiation in 10 dose-escalating fractions and verified by cell immunofluorescence. Specific markers related to the epithelial–mesenchymal transition (EMT) process and the cancer stem cell (CSC) phenotype were assessed by Western blotting. Cell invasion and migration were evaluated using Matrigel-coated transwell and wound healing assays, respectively. Nontargeted metabolomics was used to mechanistically delineate the potential metabolic patterns linked to EMT and CSCs; the CSC phenotype was also examined by sphere formation assays and cell immunofluorescence.

Results

Radioresistant oral carcinoma cell lines were successfully established and validated. These cells exhibited enhanced EMT and increase in both cell invasion and migration. These radioresistant cells further demonstrated a high metabolic profile, notably marked by lipid metabolism reprogramming and functional enrichment of ATP-binding cassette (ABC) transporters. Consistently, enhanced CSC phenotype in radioresistant cells was confirmed by elevated expression of stemness markers and increased sphere-forming capacity.

Conclusion

Radioresistant oral carcinoma cells subjected to fractionated radiation exhibit an augmented malignant phenotype. The metabolic characteristics linked to enhanced EMT and CSC phenotypes provide potential targets for improving radiotherapy in oral carcinoma.

目的某些口腔癌患者放疗失败，这意味着在治疗过程中，存活的癌细胞可能会出现更具放射抗性和侵袭性的表型。我们的研究旨在通过模拟临床相关放疗剂量策略的分次辐照方案建立放射耐受性口腔癌细胞，并研究恶性表型的全面改变。方法通过将 Cal27 和 Detroit 562 细胞暴露于 60 Gy 放射线，分 10 次剂量递增产生放射耐受性口腔癌细胞，并通过细胞免疫荧光进行验证。通过 Western 印迹法评估了与上皮-间质转化（EMT）过程和癌症干细胞（CSC）表型相关的特定标记物。细胞侵袭和迁移分别通过Matrigel包被的透孔试验和伤口愈合试验进行评估。非靶向代谢组学用于从机理上阐明与 EMT 和 CSC 相关的潜在代谢模式；CSC 表型还通过球形成试验和细胞免疫荧光进行了检测。这些细胞表现出增强的 EMT 以及细胞侵袭和迁移的增加。这些抗放射细胞进一步表现出高代谢特征，特别是脂质代谢重编程和 ATP 结合盒（ABC）转运体的功能丰富。同样，干性标志物表达的升高和球形成能力的增强也证实了耐放射细胞中CSC表型的增强。与 EMT 和 CSC 表型增强相关的代谢特征为改善口腔癌放疗提供了潜在靶点。

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引用次数: 0

Supragingival dental biofilm profile and biofilm control during orthodontic treatment with fixed orthodontic appliance: A randomized controlled trial 使用固定矫正器进行正畸治疗期间的龈上牙齿生物膜概况和生物膜控制：随机对照试验

IF 3 4区医学 Q1 Medicine

Archives of oral biology

Pub Date : 2024-04-30 DOI: 10.1016/j.archoralbio.2024.105984

T. Zibar Belasic , M. Badnjevic , M. Zigante , B. Mohar Vitezic , S. Spalj , E.P. Markova-Car

Objective

The effectiveness of supragingival dental biofilm control during orthodontic treatment and changes in the bacterial profile were analyzed.

Design

Sixty-four participants aged 12–22 years (57% female) were included in the study. Participants underwent orthodontic treatment with fixed appliances and were randomly assigned to one of the three groups, which during a period of one month: (I) used chlorhexidine digluconate (CHX), (II) used high concentration of fluoride (F) gel and (III) performed standard oral hygiene. The plaque and gingivitis index, pH of biofilm and white spot lesions (WSL) were assessed. Changes of the bacteria in the biofilm were analyzed by the quantitative polymerase chain reaction

Results

Increase in the plaque index, pH of biofilm, and WSL was observed during orthodontic treatment with standard oral hygiene. Large interindividual variability was present, and the effects of one-month use of fluorides and CHX on clinical parameters were not significant. Despite standard hygiene the abundance of studied biofilm bacteria increased - the most Streptoccocus mutans (14.2x) and S. salivarius (3.3x), moderate Veillonella parvula (3x) and the least S. sobrinus (2.3x) and Agregatibacter actinomycetemcomitans (1.9x). The use of CHX reduced S. sobrinus (2.2x) and A. actinomycetemcomitans (1.9x). Fluoride use reduced A. actinomycetemcomitans (1.3x) and S. sobrinus (1.2x). Fluorides better controlled S. mutans than CHX.

Conclusion

Bacterial biomass in supragingival biofilm increased during treatment with metal orthodontic appliances, with greater increase in cariogenic bacteria than periopathogens. Fluoride controlled S. mutans, while CHX S. sobrinus and A. actinomycetemcomitans.

目的分析正畸治疗期间龈上牙龈生物膜控制的有效性以及细菌状况的变化。设计 64 名年龄在 12-22 岁之间的参与者（57% 为女性）参加了这项研究。参加者使用固定矫治器进行正畸治疗，并被随机分配到三组中的一组：(I)使用洗必泰二氯葡萄糖酸盐（CHX），(II)使用高浓度氟化物（F）凝胶，(III)进行标准口腔卫生。对牙菌斑和牙龈炎指数、生物膜的 pH 值和白斑病变（WSL）进行了评估。通过定量聚合酶链反应分析了生物膜中细菌的变化。结果在使用标准口腔卫生方法进行正畸治疗期间，观察到牙菌斑指数、生物膜 pH 值和白斑病变（WSL）均有所上升。个体之间的差异很大，使用一个月的氟化物和 CHX 对临床参数的影响并不显著。尽管采取了标准的口腔卫生措施，但所研究的生物膜细菌的数量还是有所增加--最多的是变异链球菌（14.2 倍）和唾液酸链球菌（3.3 倍），中等的是副龈维龙菌（3 倍），最少的是梭状芽孢杆菌（2.3 倍）和放线菌（1.9 倍）。使用 CHX 可减少 S. sobrinus（2.2 倍）和 A. actinomycetemcomitans（1.9 倍）。使用氟化物可减少放线菌（1.3 倍）和 S. sobrinus（1.2 倍）。结论在金属正畸矫治器治疗期间，龈上牙龈生物膜中的细菌生物量增加，致龋细菌比周病原菌增加更多。氟化物可控制 S. mutans，而 CHX 可控制 S. sobrinus 和 A. actinomycetemcomitans。

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引用次数: 0

Insights into medieval rural lives: A paleo-odontological investigation of two central European communities 洞察中世纪农村生活：对两个中欧社区的古牙科调查

IF 3 4区医学 Q1 Medicine

Archives of oral biology

Pub Date : 2024-04-30 DOI: 10.1016/j.archoralbio.2024.105985

Antonella Pedergnana , Roger Seiler , Renata Huber , Patrick Eppenberger , Frank Rühli

Objective

Oral status is an important indicator of past lifestyles. Determining the presence and extent of oral pathologies helps reconstruct average oral health, paramasticatory activities and diet of ancient and historical populations.

Design

In this study, the dental remains from the early medieval cemetery of Früebergstrasse in Baar (Canton of Zug, Switzerland) and the high medieval Dalheim cemetery (North Rhine-Westphalia, Germany) were analyzed. Caries, periodontal condition, periapical lesions, antemortem tooth loss, and enamel hypoplasia were assessed in 654 teeth (993 observable loci) from 68 individuals (Baar: n = 36; Dalheim: n = 32).

Results

The oral status of both populations was affected by age with higher values of tooth wear in advanced age individuals. High tooth wear values in both populations point towards the consumption of abrasive foods. Pronounced anterior tooth wear in Baar may also be due to non-masticatory tooth usage. Finally, possible nutritional deficiencies were hypothesized for the Baar population. A higher caries prevalence was observed in the Baar group, probably due to differences in carbohydrate intake. The oral conditions observed in the two studied populations exhibited several analogies, suggesting comparable lifestyles despite their separation in space and time. The only differences observed are related to the use of teeth as “tools” and are thus determined by behavioral choices rather than diverse socioeconomic factors.

Conclusions

Using multiple dental parameters to examine the oral health of premodern individuals can provide useful insights into the interactions between humans and their environment, from dietary patterns to paramasticatory activities.

目标口腔状况是过去生活方式的一个重要指标。本研究分析了巴尔（瑞士楚格州）Früebergstrasse 中世纪早期墓地和达尔海姆（德国北莱茵-威斯特法伦州）中世纪早期墓地的牙齿遗存。对 68 人（巴尔：36 人；达尔海姆：32 人）的 654 颗牙齿（993 个可观察到的位点）的龋齿、牙周状况、根尖周病变、死前牙齿脱落和釉质发育不全情况进行了评估。两个人群的牙齿磨损值都很高，这表明他们都食用了磨蚀性食物。巴尔人的前牙磨损明显也可能是由于使用非咀嚼性牙齿造成的。最后，我们假设巴尔人可能存在营养不良。据观察，巴尔族的龋齿发生率较高，这可能是由于碳水化合物摄入量的差异造成的。在所研究的两个人群中观察到的口腔状况有几处相似之处，这表明尽管两个人群在时间和空间上相隔遥远，但他们的生活方式却具有可比性。所观察到的唯一差异与将牙齿用作 "工具 "有关，因此是由行为选择而非不同的社会经济因素决定的。

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引用次数: 0

Influence of root shape on canal complexity at the mandibular molar apical surgical resection level: A micro-CT study 牙根形状对下颌磨牙根尖手术切除水平的牙管复杂性的影响：显微 CT 研究

IF 3 4区医学 Q1 Medicine

Archives of oral biology

Pub Date : 2024-04-27 DOI: 10.1016/j.archoralbio.2024.105983

António Ginjeira , Filipa Neto , Shirin Behdad , Inês Farinha , Nuno Gonçalves , Jorge N.R. Martins , Mariana Pires , Mário Rito Pereira , Isabel Vasconcelos , Isabel Duarte

Objectives

This study aimed to investigate the relationship between the aspect ratios of mandibular molar roots at the apical 3-mm level and their root canal complexity.

Design

This study used micro-CT imaging to analyze 163 two-rooted mandibular molars. The aspect ratios of the roots at the apical 3-mm level were categorized as "< 2.75" or "≥ 2.75" (mesial) and "< 1.75" or "≥ 1.75" (distal). A two-dimensional (2D) analysis focused on four apical axial cross-section levels to determine the presence of main and accessory canals and isthmus. Additionally, a three-dimensional (3D) assessment of the apical 4-mm of both roots examined main and accessory canals, apical foramina, apical deltas, and middle mesial canals.

Results

Mesial roots with aspect ratios ≥ 2.75 showed a higher number of main canals at all levels compared to those with aspect ratios < 2.75 at the 3-mm level. Additionally, the ≥ 2.75 group exhibited more accessory canals and a higher average number of accessory canals. The 3D assessment confirmed significantly more accessory canals and apical foramina in the ≥ 2.75 group. The prevalence of roots with apical deltas was nearly double in the ≥ 2.75 group, and middle mesial canals were exclusively found in this group. In the distal root, the ≥ 1.75 group showed a significantly higher number of main canals at all axial levels. No significant differences were observed between groups in terms of accessory canals, apical foramina, or deltas.

Conclusions

A higher root aspect ratio is related to higher anatomical complexity.

目的本研究旨在探讨下颌磨牙根尖 3 mm 水平的长宽比与其根管复杂性之间的关系。设计本研究使用显微 CT 成像技术分析了 163 颗双根下颌磨牙。根尖 3 毫米处牙根的长宽比分为"< 2.75 "或"≥ 2.75"（中侧）和"< 1.75 "或"≥ 1.75"（远侧）。二维（2D）分析侧重于四个根尖轴向横截面水平，以确定是否存在主牙道、附属牙道和峡部。此外，还对两个牙根顶端 4 毫米处进行了三维（3D）评估，检查了主根管和附属根管、根尖孔、根尖三角区和中中层根管。结果与 3 毫米水平的纵横比为 < 2.75 的牙根相比，纵横比≥ 2.75 的中中层牙根在所有水平上都显示出较多的主根管。此外，纵横比≥2.75组显示出更多的附属管，附属管的平均数量也更高。三维评估证实，≥ 2.75 组的附属根管和根尖孔明显更多。在≥2.75组中，根尖三角洲的发生率几乎翻了一番，而且该组中只发现了中中根。在牙根远端，≥ 1.75 组在所有轴向水平上的主根管数量都明显较多。结论较高的牙根纵横比与较高的解剖复杂性有关。

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引用次数: 0

Reduction of microorganisms in carious dentin by photodynamic therapy mediated by potassium iodide added to methylene blue and red laser 在亚甲蓝和红色激光中加入碘化钾，通过光动力疗法减少龋齿牙本质中的微生物

IF 3 4区医学 Q1 Medicine

Archives of oral biology

Pub Date : 2024-04-26 DOI: 10.1016/j.archoralbio.2024.105978

Alejandra Brenes-Alvarado , Felipe Fabrício Farias-da-Silva , Jorge Rodrigo Soto-Montero , Juliana Benine-Warlet , Francisco Carlos Groppo , Carolina Steiner-Oliveira

Objective

To evaluate the effect of the association of potassium iodide to antimicrobial photodynamic therapy on human carious dentin produced with a microcosm biofilm model.

Methods

A microcosm biofilm model was used to generate a caries lesion on human dentin. Pooled human saliva diluted with glycerol was used as an inoculum on specimens immersed on McBain artificial saliva enriched with 1 % sucrose (24 h at 37 °C in 5 % CO₂). After refreshing culture media for 7 days, the dentin specimens were divided in 5 groups (3 specimens per group, in triplicate; n = 9): C (NaCl 0.9 %), CX (2 % chlorhexidine), PKI (0.01 % methylene blue photosensitizer+50 mM KI), L (laser at 15 J, 180 s, 22.7 J/cm²), and PKIL (methylene blue + KI + Laser). After the treatments, dentin was collected, and a 10-fold serial dilution was performed. The number of total microorganisms, total lactobacilli, total streptococci, and Streptococcus mutans was analyzed by microbial counts (CFU/mL). After normality and homoscedasticity analysis, the Welch's ANOVA and Dunnett's tests were used for CFU. All tests used a 5 % significance level.

Results

CX and PKIL groups showed significant bacterial decontamination of dentin, compared to group C (p < 0.05) reaching reductions up to 3.8 log₁₀ for CX for all microorganisms’ groups and PKIL showed 0.93, 1.30, 1.45, and 1.22 log₁₀ for total microorganisms, total lactobacilli, total streptococci, and S. mutans, respectively.

Conclusion

aPDT mediated by the association of KI and methylene blue with red laser reduced the viability of microorganisms from carious dentin and could be a promising option for cavity decontamination.

目的评价碘化钾与抗菌光动力疗法联合使用对利用微宇宙生物膜模型生成的人类龋齿牙本质的影响。方法利用微宇宙生物膜模型在人类牙本质上生成龋损。用甘油稀释的人类唾液作为接种体，将标本浸泡在富含 1% 蔗糖的 McBain 人工唾液中（37 °C、5% CO2 条件下 24 小时）。刷新培养基 7 天后，将牙本质标本分为 5 组（每组 3 个标本，一式三份；n = 9）：C组（0.9%氯化钠）、CX组（2%洗必泰）、PKI组（0.01%亚甲基蓝光敏剂+50 mM KI）、L组（激光，15 J，180 s，22.7 J/cm2）和PKIL组（亚甲基蓝+KI+激光）。处理后，收集牙本质并进行 10 倍序列稀释。通过微生物计数（CFU/mL）分析总微生物数、总乳酸菌数、总链球菌数和变异链球菌数。在进行正态性和同方差分析后，对 CFU 采用韦尔奇方差分析和邓尼特检验。结果与 C 组相比，CX 组和 PKIL 组对牙本质的细菌净化效果显著（p <0.05），CX 组所有微生物的减少量达 3.8 log10，PKIL 组的减少量分别为 0.93、1.30、1.45 和 1.22 log10。总微生物、总乳酸杆菌、总链球菌和变异单胞菌分别为 0.93、1.30、1.45 和 1.22 log10。

{"title":"Reduction of microorganisms in carious dentin by photodynamic therapy mediated by potassium iodide added to methylene blue and red laser","authors":"Alejandra Brenes-Alvarado , Felipe Fabrício Farias-da-Silva , Jorge Rodrigo Soto-Montero , Juliana Benine-Warlet , Francisco Carlos Groppo , Carolina Steiner-Oliveira","doi":"10.1016/j.archoralbio.2024.105978","DOIUrl":"https://doi.org/10.1016/j.archoralbio.2024.105978","url":null,"abstract":"<div><h3>Objective</h3><p>To evaluate the effect of the association of potassium iodide to antimicrobial photodynamic therapy on human carious dentin produced with a microcosm biofilm model.</p></div><div><h3>Methods</h3><p>A microcosm biofilm model was used to generate a caries lesion on human dentin. Pooled human saliva diluted with glycerol was used as an inoculum on specimens immersed on McBain artificial saliva enriched with 1 % sucrose (24 h at 37 °C in 5 % CO<sub>2</sub>). After refreshing culture media for 7 days, the dentin specimens were divided in 5 groups (3 specimens per group, in triplicate; n = 9): C (NaCl 0.9 %), CX (2 % chlorhexidine), PKI (0.01 % methylene blue photosensitizer+50 mM KI), L (laser at 15 J, 180 s, 22.7 J/cm<sup>2</sup>), and PKIL (methylene blue + KI + Laser). After the treatments, dentin was collected, and a 10-fold serial dilution was performed. The number of total microorganisms, total lactobacilli, total streptococci, and <em>Streptococcus mutans</em> was analyzed by microbial counts (CFU/mL). After normality and homoscedasticity analysis, the Welch's ANOVA and Dunnett's tests were used for CFU. All tests used a 5 % significance level.</p></div><div><h3>Results</h3><p>CX and PKIL groups showed significant bacterial decontamination of dentin, compared to group C (p < 0.05) reaching reductions up to 3.8 log<sub>10</sub> for CX for all microorganisms’ groups and PKIL showed 0.93, 1.30, 1.45, and 1.22 log<sub>10</sub> for total microorganisms, total lactobacilli, total streptococci, and <em>S. mutans</em>, respectively.</p></div><div><h3>Conclusion</h3><p>aPDT mediated by the association of KI and methylene blue with red laser reduced the viability of microorganisms from carious dentin and could be a promising option for cavity decontamination.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140878749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hyaluronidase inhibits TGF-β-mediated rat periodontal ligament fibroblast expression of collagen and myofibroblast markers: An in vitro exploration of periodontal tissue remodeling 透明质酸酶抑制 TGF-β 介导的大鼠牙周韧带成纤维细胞表达胶原和肌成纤维细胞标记物：牙周组织重塑的体外探索

IF 3 4区医学 Q1 Medicine

Archives of oral biology

Pub Date : 2024-04-26 DOI: 10.1016/j.archoralbio.2024.105980

Junlin Li , Chen Chen , Yunting Zeng , Jiaqi Lu , Liwei Xiao

Objective

To determine the effect of hyaluronic acid (HA) degradation by hyaluronidase (HYAL) in inhibiting collagen fiber production by rat periodontal ligament cells (rPDLCs).

Design

Primary rPDLCs were isolated from the euthanized rats and used for in vitro experiments. The appropriate HYAL concentration was determined through CCK-8 testing for cytotoxicity detection and Alizarin red staining for mineralization detection. RT-qPCR and western blot assays were conducted to assess the effect of HYAL, with or without TGF-β, on generation of collagen fiber constituents and expression of actin alpha 2, smooth muscle (ACTA2) of rPDLCs.

Results

Neither cell proliferation nor mineralization were significantly affected by treatment with 4 U/mL HYAL. HYAL (4 U/mL) alone downregulated type I collagen fiber (Col1a1 and Col1a2) and Acta2 mRNA expression; however, ACTA2 and COL1 protein levels were only downregulated by HYAL treatment after TGF-β induction.

Conclusions

Treatment of rPDLCs with HYAL can inhibit TGF-β-induced collagen matrix formation and myofibroblast transformation.

目的确定透明质酸（HA）被透明质酸酶（HYAL）降解对抑制大鼠牙周韧带细胞（rPDLCs）产生胶原纤维的影响。通过 CCK-8 检测细胞毒性和茜素红染色检测矿化度来确定适当的 HYAL 浓度。结果4 U/mL的HYAL处理对细胞增殖和矿化均无明显影响。结论用 HYAL 处理 rPDLCs 可抑制 TGF-β 诱导的胶原基质形成和肌成纤维细胞转化。

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引用次数: 0

Effect of hydroxyapatite nanoparticles coating of titanium surface on biofilm adhesion: An in vitro study 钛表面羟基磷灰石纳米颗粒涂层对生物膜粘附性的影响：体外研究

IF 3 4区医学 Q1 Medicine

Archives of oral biology

Pub Date : 2024-04-26 DOI: 10.1016/j.archoralbio.2024.105986

Gilda Rocha dos Reis-Neta , Antônio Pedro Ricomini-Filho , Loyse Martorano-Fernandes , Vanessa Felipe Vargas-Moreno , Altair Antoninha Del Bel Cury , Raissa Micaella Marcello-Machado

Aim

To evaluate the adhesion of mono and duospecies biofilm on a commercially available dental implant surface coated with hydroxyapatite nanoparticles (nanoHA).

Material and methods

Titanium discs were divided into two groups: double acid-etched (AE) and AE coated with nanoHA (NanoHA). Surface characteristics evaluated were morphology, topography, and wettability. Mono and duospecies biofilms of Streptococcus sanguinis (S. sanguinis) and Candida albicans (C. albicans) were formed. Discs were exposed to fetal bovine serum (FBS) to form the pellicle. Biofilm was growth in RPMI1640 medium with 10% FBS and 10% BHI medium for 6 h. Microbial viability was evaluated using colony-forming unit and metabolic activity by a colorimetric assay of the tetrazolium salt XTT. Biofilm architecture and organization were evaluated by confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM).

Results

AE surface had more pores, while NanoHA had even nanoHA crystals distribution. Roughness was similar (AE: 0.59 ± 0.07 µm, NanoHA: 0.69 ± 0.18 µm), but wettability was different (AE: Θw= 81.79 ± 8.55°, NanoHA: Θw= 53.26 ± 11.86°; P = 0.01). NanoHA had lower S. sanguinis viability in monospecies biofilm (P = 0.007). Metabolic activity was similar among all biofilms. In SEM both surfaces on C. albicans biofilm show a similar distribution of hyphae in mono and duospecies biofilms. AE surface has more S. sanguinis than the NanoHA surface in the duospecies biofilm. CLSM showed a large proportion of live cells in all groups.

Conclusions

The nanoHA surface reduced the adhesion of S. sanguinis biofilm but did not alter the adhesion of C. albicans or the biofilm formed by both species.

材料和方法将钛盘分为两组：双酸蚀（AE）和 AE 涂层纳米HA（NanoHA）。评估的表面特征包括形态、形貌和润湿性。血链球菌（S. sanguinis）和白色念珠菌（C. albicans）形成了单种和双种生物膜。将圆片置于胎牛血清（FBS）中以形成细胞膜。生物膜在含有 10% FBS 和 10% BHI 培养基的 RPMI1640 培养基中生长 6 小时。共焦激光扫描显微镜（CLSM）和扫描电子显微镜（SEM）对生物膜的结构和组织进行了评估。粗糙度相似（AE：0.59 ± 0.07 µm；NanoHA：0.69 ± 0.18 µm），但润湿性不同（AE：Θw= 81.79 ± 8.55°；NanoHA：Θw= 53.26 ± 11.86°；P = 0.01）。NanoHA 在单菌种生物膜中的 S. sanguinis 存活率较低（P = 0.007）。所有生物膜的代谢活性相似。在扫描电子显微镜下，白僵菌生物膜的两个表面在单种和双种生物膜中的菌丝分布相似。在双种生物膜中，AE 表面比 NanoHA 表面有更多的 S. sanguinis。结论纳米HA 表面减少了 S. sanguinis 生物膜的粘附力，但没有改变白僵菌的粘附力或这两种菌形成的生物膜。

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引用次数: 0

Identification of ACAA1 and HADHB as potential prognostic biomarkers based on a novel fatty acid oxidation-related gene model in head and neck squamous cell carcinoma: A retrospective study 基于新型脂肪酸氧化相关基因模型的头颈部鳞状细胞癌潜在预后生物标志物 ACAA1 和 HADHB 的鉴定：一项回顾性研究

IF 3 4区医学 Q1 Medicine

Archives of oral biology

Pub Date : 2024-04-24 DOI: 10.1016/j.archoralbio.2024.105982

Wan-li Wang , Hong-jie Jiang , Ze-liang Shen , Ya-ling Tang , Jian Jiang , Xin-hua Liang

Objectives

To investigate the importance of fatty acid oxidation (FAO)-related genes in predicting the progression and prognosis of head and neck squamous cell carcinoma (HNSCC).

Methods

The FAO-related gene prognostic model was established employing Cox regression analyses, during which accuracy and sensitivity of the gene model were evaluated in The Cancer Genome Atlas (TCGA) internal testing and Gene Expression Omnibus (GEO) external validation cohorts. Ultimately, hub genes were identified among 13 model genes using STRING and Cytoscape, with preliminary validation carried out through immunohistochemistry.

Results

The model, which comprised 13 genes (ABCD2, ACAA1, ACACB, AKT1, CNR1, CPT1C, CROT, ECHDC2, ETFA, HADHB, IRS2, LONP2, and SLC25A17), was established. On the basis of the median risk score, the two cohorts were grouped into low-and high-risk groups in the subsequent test and validation, and the former exhibited significantly higher survival rates than the latter. Nomograms were established based on prognostic factors, including stage and risk score, and individualized for the prediction of HNSCC patients. Ultimately, immunohistochemical staining showed that ACAA1 and HADHB were significantly under-expressed in HNSCC, with a favorable prognosis associated with low HADHB and high ACAA1.

Conclusions

The gene prognostic model has illustrated promising capability in predicting the prognosis, and ACAA1 and HADHB might serve as potential therapeutic biomarkers for HNSCC patients.

目的研究脂肪酸氧化（FAO）相关基因在预测头颈部鳞状细胞癌（HNSCC）的进展和预后中的重要性。方法利用Cox回归分析建立FAO相关基因预后模型，并在癌症基因组图谱（TCGA）内部测试和基因表达总库（GEO）外部验证队列中评估基因模型的准确性和敏感性。结果该模型由 13 个基因（ABCD2、ACAA1、ACACB、AKT1、CNR1、CPT1C、CROT、ECHDC2、ETFA、HADHB、IRS2、LONP2 和 SLC25A17）组成。在随后的测试和验证中，根据中位风险评分将两个队列分为低风险组和高风险组，前者的存活率明显高于后者。根据预后因素（包括分期和风险评分）建立了提名图，并对 HNSCC 患者进行个体化预测。最终，免疫组化染色显示，ACAA1和HADHB在HNSCC中明显低表达，低HADHB和高ACAA1预后良好。

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引用次数: 0

Cervical wear pathobiology by robot-simulated 3-year toothbrushing – New methodological approach 通过机器人模拟 3 年刷牙进行宫颈磨损病理生物学研究--新的方法学途径

IF 3 4区医学 Q1 Medicine

Archives of oral biology

Pub Date : 2024-04-24 DOI: 10.1016/j.archoralbio.2024.105981

K. Wilke , S. Nietzsche , M. Hemmleb , S. Mason , R. Varghese , T. Lang , P. Gaengler

Objectives

An ex-vivo study was aimed at (i) programming clinically validated robot three-year random toothbrushing, (ii) evaluating cervical macro- and microwear patterns on all tooth groups of different functional age, (iii) documenting and codificating wear related morphological features at the cemento-enamel junction in young teeth and on roots in older teeth.

Design

Following ethical approval random toothbrushing (44 strokes per tooth horizontally, rotating, vertically; 2x/d) with manual toothbrushes and low-abrasive dentifrice was performed in an artificial oral cavity with brushing-force 3.5 N on 14 extracted human teeth. Morphological features were examined by SEM at baseline and after simulated 3 years using the replication technique. 3D-SEM analyses were carried out with a four-quadrant back scattered electron detector. Wilcoxon-Mann-Whitney-test was used for statistical analyses.

Results

3-year random toothbrushing with horizontal, rotating and vertical brushing movements revealed morphological features classified as four enamel patterns, one dentin pattern and three cervical patterns. Negative impacts were enamel, cementum and dentin loss. Positive impact on oral health was removing dental calculus and straightening cervical traumatic and iatrogenic damages. The volume loss varied from $\overset{̅}{x} = 34.25 nl$ to $\overset{̅}{x} = 87.75 nl .$ Wear extended apically from 100 to 1500 micrometres.

Conclusion

Robot simulated toothbrushing in an artificial oral cavity, with subsequent SEM and 3D-SEM assessment, elucidated both negative and oral health-contributing micromorphology patterns of cervical wear after simulated 3-year random toothbrushing. Cervical macro- and microwear of cementum revealed, for the first time, what we describe as overhanging enamel peninsulas and enamel islands on roots in young teeth, but no enamel islands on roots from older teeth after root cementum loss. In contrast, many older teeth exhibited enamel peninsulas.

目的一项体外研究的目的是：(i) 对经过临床验证的机器人三年随机刷牙进行编程；(ii) 评估不同功能年龄的所有牙群的牙颈部宏观和微观磨损模式；(iii) 记录和编纂年轻牙齿和年长牙齿根部牙釉质交界处与磨损相关的形态特征。设计在获得伦理批准后，使用手动牙刷和低磨损性牙膏在人工口腔中对 14 颗拔出的人类牙齿进行随机刷牙（每颗牙齿水平、旋转、垂直刷 44 次；2x/d），刷牙力为 3.5 N。使用 SEM 扫描电子显微镜检查了基线和模拟 3 年后的形态特征。使用四象限背散射电子探测器进行 3D-SEM 分析。结果3年的随机刷牙，采用水平、旋转和垂直刷牙运动，发现的形态特征分为四种釉质形态、一种牙本质形态和三种牙颈部形态。负面影响是牙釉质、牙本质和牙本质脱落。对口腔健康的积极影响是清除牙结石，矫正牙颈创伤和先天性损伤。体积损失从 x̅=34.25nl 到 x̅=87.75nl 不等。结论机器人在人工口腔中模拟刷牙，随后进行扫描电镜和三维扫描电镜评估，阐明了在模拟随机刷牙 3 年后牙颈部磨损的负面和口腔健康贡献微观形态。牙颈部骨水泥的宏观和微观磨损首次在年轻牙齿的牙根上发现了我们所描述的悬垂釉质半岛和釉质岛，但在牙根骨水泥脱落后，较老牙齿的牙根上没有发现釉质岛。与此相反，许多较老的牙齿表现出珐琅质半岛。

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引用次数: 0

Verbascoside inhibits oral squamous cell carcinoma cell proliferation, migration, and invasion by the methyltransferase 3-mediated microRNA-31-5p/homeodomain interacting protein kinase 2 axis 马鞭草甙通过甲基转移酶 3 介导的 microRNA-31-5p/homeodomain 交互蛋白激酶 2 轴抑制口腔鳞状细胞癌细胞增殖、迁移和侵袭

IF 3 4区医学 Q1 Medicine

Archives of oral biology

Pub Date : 2024-04-22 DOI: 10.1016/j.archoralbio.2024.105979

Yuhua Huang, Wei Wu, Xing Zhang

Objective

The study aimed to investigate the effects of verbascoside on oral squamous cell carcinoma (OSCC) cellular behaviors and underlying molecular mechanisms.

Design

For this purpose, SCC9 and UM1 cell lines were treated with verbascoside, and their biological behaviors, including proliferation, migration, and invasion, were evaluated using cell counting kit-8, 5-Ethynyl-2′-deoxyuridine, and Transwell assays. The expression of methyltransferase-3 (METTL3), microRNA (miR)− 31–5p, and homeodomain interacting protein kinase-2 (HIPK2) were examined using quantitative real-time polymerase chain reaction (qRT-PCR). The interaction between METTL3 and miR-31–5p was evaluated by RNA immunoprecipitation and methylated RNA immunoprecipitation, while the interaction between miR-31–5p and HIPK2 was evaluated by dual-luciferase reporter analysis.

Results

The results indicated inhibition of OSCC cell proliferation, migration, and invasion post verbascoside treatment. Similarly, METTL3 was upregulated in OSCC cells and was inhibited post-verbascoside treatment. Overexpressing METTL3 promoted the cellular processes. Moreover, miR-31–5p was upregulated in OSCC cells, where METTL3 facilitated the processing of miR-31–5p in an N6-methyladenosine (m6A)-dependent manner. The HIPK2 served as miR-31–5p target, where overexpressing miR-31–5p or HIPK2 knockdown reversed the suppression of verbascoside-induced biological behaviors.

Conclusions

Verbascoside inhibited the progression of OSCC by inhibiting the METTL3-regulated miR-31–5p/HIPK2 axis. These findings suggested that verbascoside might be an effective drug for OSCC therapy.

目的本研究旨在探讨马鞭草苷对口腔鳞状细胞癌（OSCC）细胞行为的影响及其潜在的分子机制。为此，研究人员用马鞭草苷处理了SCC9和UM1细胞系，并用细胞计数试剂盒-8、5-乙炔基-2′-脱氧尿苷和Transwell试验评估了它们的生物学行为，包括增殖、迁移和侵袭。使用实时定量聚合酶链反应（qRT-PCR）检测了甲基转移酶-3（METTL3）、microRNA（miR）- 31-5p和同源结构域相互作用蛋白激酶-2（HIPK2）的表达。通过 RNA 免疫沉淀和甲基化 RNA 免疫沉淀评估了 METTL3 与 miR-31-5p 之间的相互作用，而通过双荧光素酶报告分析评估了 miR-31-5p 与 HIPK2 之间的相互作用。同样，METTL3 在 OSCC 细胞中上调，并在马鞭草苷处理后受到抑制。过表达 METTL3 会促进细胞进程。此外，miR-31-5p 在 OSCC 细胞中上调，其中 METTL3 以 N6-甲基腺苷（m6A）依赖的方式促进了 miR-31-5p 的处理。HIPK2是miR-31-5p的靶点，过表达miR-31-5p或敲除HIPK2可逆转对马鞭草苷诱导的生物学行为的抑制。这些研究结果表明，马鞭草苷可能是治疗OSCC的有效药物。

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引用次数: 0