Archives of oral biology最新文献_第6页

Retraction notice to “Berberine reduces inflammation of human dental pulp fibroblast via miR-21/KBTBD7 axis” [Archives of Oral Biology 110 (2020) 104630] “小檗碱通过miR-21/KBTBD7轴降低人牙髓成纤维细胞炎症”的撤稿通知[口腔生物学文献110(2020)104630]。

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2026-02-01 Epub Date: 2025-12-03 DOI: 10.1016/j.archoralbio.2025.106464

Song Jia , Wu Qishan , Jiang Jin , Sun Degang , Wang Fang , Xin Bingchang , Cui Qi

引用次数: 0

Impact of stress on pain sensitization and emotional responses in a rat model of persistent TMJ inflammation 应激对持续性颞下颌关节炎症大鼠模型疼痛敏感性和情绪反应的影响

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2026-02-01 Epub Date: 2025-11-19 DOI: 10.1016/j.archoralbio.2025.106463

Jardel Francisco Mazzi-Chaves , Ana Paula Ribeiro Novaes , Glauce Crivelaro Nascimento , Christie Ramos Andrade Leite-Panissi

Objective

Temporomandibular disorders (TMDs) are frequently associated with psychological distress, including anxiety and depression, which can modulate pain perception and sensitivity. This study investigated the impact of acute and chronic stress on the progression of temporomandibular joint (TMJ) pain, secondary hyperalgesia, and stress-induced affective disturbances.

Design

Using a well-established preclinical model, Wistar-Hannover rats underwent persistent TMJ inflammation via intra-articular administration of Freund’s complete adjuvant (CFA). Orofacial mechanical allodynia and secondary hyperalgesia were assessed using von Frey test in the orofacial region and hot plate test in the hind paw. To evaluate the influence of stress on affective behaviors, Acute stress (AS), chronic restraint stress (CRS) and Unpredictable chronic stress (UCS) paradigms were implemented, followed by behavioral assessments using the elevated plus maze (EPM), open field (OF), and sucrose preference tests.

Results

Results demonstrated that chronic stress exacerbated CFA-induced orofacial allodynia. TMJ inflammation induced secondary hyperalgesia, with AS partially restoring baseline nociception, while UCS amplified central sensitization. Notably, CRS did not influence hind paw nociception in CFA-injected rats. Behavioral analyses revealed that CFA injection heightened anxiety-like behavior by decreased open-arm exploration. Acute stress further intensified anxiety and impaired exploratory activity, whereas chronic stresses significantly worsened both anxiety- and depression-like behaviors.

Conclusion

These findings underscore the complex interplay between stress and pain processing in TMDs, highlighting the detrimental role of chronic stress in exacerbating pain sensitivity and emotional dysregulation. Understanding these mechanisms could lead to more effective, targeted treatments, improving patient outcomes.

目的颞下颌疾病（TMDs）常伴有焦虑、抑郁等心理困扰，可调节疼痛感知和敏感性。本研究探讨了急性和慢性应激对颞下颌关节（TMJ）疼痛、继发性痛觉过敏和应激性情感障碍进展的影响。设计采用成熟的临床前模型，Wistar-Hannover大鼠通过关节内给予Freund 's完全佐剂（CFA）进行持续性TMJ炎症。采用von Frey试验和后爪热板试验对大鼠口面部机械异常性痛和继发性痛觉过敏进行评估。为了评估应激对情感行为的影响，本研究采用急性应激（AS）、慢性约束应激（CRS）和不可预测慢性应激（UCS）范式，并采用升高+迷宫（EPM）、开阔场（of）和蔗糖偏好测试进行行为评估。结果慢性应激加重cfa诱导的口面部异常痛。颞下颌关节炎症引起继发性痛觉过敏，AS部分恢复基线痛觉，而UCS放大中枢致敏。值得注意的是，CRS对注射cfa的大鼠后爪痛觉没有影响。行为分析显示，CFA注射通过减少张开臂探查而增加焦虑样行为。急性应激进一步加剧了焦虑和探索性活动，而慢性应激显著恶化了焦虑和抑郁样行为。结论这些发现强调了应激与疼痛加工之间复杂的相互作用，强调了慢性应激在加剧疼痛敏感性和情绪失调中的有害作用。了解这些机制可以带来更有效、更有针对性的治疗，改善患者的预后。

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引用次数: 0

Effect of phenotype switched Candida auris mono-culture and co-culture biofilms on the morphology, viability, and adhesion of hTERT TIGKs and ORL-48 cell lines 表型转换耳念珠菌单培养和共培养生物膜对hTERT TIGKs和ORL-48细胞株形态、活力和粘附的影响

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2026-02-01 Epub Date: 2025-12-09 DOI: 10.1016/j.archoralbio.2025.106478

Mukarramah Zainal , Nurul ‘Izzah Mohd Sarmin , Mohammad Johari Ibrahim , Nicola Cirillo , Stuart G. Dashper , Mohd Hafiz Arzmi

Objectives

This study aims to determine the paracrine effects of Candida auris phenotypic switching in mono- and co-culture with Staphylococcus aureus on oral epithelial homeostasis and oncogenic progression in phenotypically normal (hTERT TIGKs) and malignant (ORL-48) oral keratinocytes.

Design

C. auris switched phenotype was scored using Phloxine B, and mono- and co-culture biofilms with S. aureus were developed. hTERT TIGKs and ORL-48 cell lines were independently seeded into 6-well and 96-well plates for dispase and viability test, respectively. The oral cell lines were exposed to phenotypically switched C. auris mono- and co-culture biofilm test cell growth medium (TCGM) for 24 h. Outcomes included cell morphology, metabolic activity/viability (CCK-8), and cell–cell adhesion (dispase assay).

Results

Microscopic observation revealed that the biofilm induced damage and disrupted epithelial cell integrity in a paracrine manner. The mono- and co-culture TCGM suppressed the growth of normal cells while promoting the metabolic activity of cancer cells. The adhesion analysis of hTERT TIGKs indicated a strong intercellular cohesion, while ORL-48 cells downregulated intercellular adhesion and compromised cell-cell cohesion.

Conclusion

C. auris biofilms promote the development of a malignant phenotype by regulating cell viability, promoting epithelial-mesenchymal transition, and adhesion in a switched generation-dependent manner.

目的：本研究旨在确定金黄色葡萄球菌单培养和共培养时耳念珠菌表型转换对口腔上皮稳态和表型正常（hTERT TIGKs）和恶性（ORL-48）口腔角质形成细胞的癌性进展的旁分泌作用。设计：利用苯氧辛B对金黄色葡萄球菌的开关表型进行评分，并与金黄色葡萄球菌进行单培养和共培养生物膜的制备。hTERT TIGKs和ORL-48细胞系分别独立接种于6孔和96孔板中进行疾病和活力检测。将口腔细胞系暴露于表型切换的耳念珠菌单一和共培养生物膜试验细胞生长培养基（TCGM）中24 h。结果包括细胞形态、代谢活性/活力（CCK-8）和细胞-细胞粘附（疾病测定）。结果：显微镜观察显示，生物膜以旁分泌方式诱导上皮细胞损伤和破坏细胞完整性。单独培养和共培养的TCGM抑制正常细胞的生长，同时促进癌细胞的代谢活性。hTERT TIGKs的粘附分析表明，细胞间黏附较强，而ORL-48细胞的细胞间黏附下调，细胞间黏附受损。结论：耳念珠菌生物膜通过调节细胞活力、促进上皮-间质转化和粘附，以一种世代依赖的方式促进恶性表型的发展。

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引用次数: 0

BBI608 induces apoptosis in mucoepidermoid carcinoma cells by targeting a post-transcriptional regulatory mechanisms of myeloid cell leukemia-1 BBI608通过靶向髓细胞白血病-1的转录后调控机制诱导黏液表皮样癌细胞凋亡

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2026-02-01 Epub Date: 2025-12-04 DOI: 10.1016/j.archoralbio.2025.106467

Da-In Choi , Hyun-Ji Kim , Dong-Guk Park , Jae-Han Lee , Thantrira Porntaveetus , Sung-Dae Cho

Objectives

BBI608 has demonstrated antitumor activity in several human cancers. However, its efficacy against mucoepidermoid carcinoma (MEC) remains unexplored. This study investigated the antitumor potential of BBI608 in MEC cell lines.

Design

The antitumor activity of BBI608 in MC3 and YD-15 mucoepidermoid carcinoma (MEC) cell lines was assessed using trypan blue exclusion, Live/Dead, and sphere formation assays. Apoptotic effects were investigated via western blotting, 4′,6-diamidino-2-phenylindole (DAPI) staining, Annexin V-fluorescein isothiocyanate/propidium iodide (V-FITC/PI) double staining, and reverse transcription-quantitative PCR.

Results

BBI608 exhibited growth-inhibitory effects in MEC cell lines, decreasing cell viability and sphere formation capacity while increasing cell death. BBI608-induced apoptosis was confirmed by increased cleaved caspase-3 and PARP expression, nuclear morphological changes characteristic of apoptosis, and increased Annexin V positivity. Furthermore, BBI608 significantly downregulated Mcl-1 expression, which contributed to apoptosis induction in MEC cells. This Mcl-1 downregulation appeared to be mediated by both proteasome-dependent protein degradation and translational regulatory mechanisms in MC3 and YD-15 cells, respectively.

Conclusion

These findings demonstrate that BBI608 effectively inhibits MEC cell proliferation in vitro by inducing Mcl-1-dependent apoptosis. This suggests BBI608 warrants further investigation as a potential therapeutic agent for MEC.

目的bbi608在几种人类癌症中显示出抗肿瘤活性。然而，其对黏液表皮样癌（MEC）的疗效尚不清楚。本研究考察了BBI608在MEC细胞系中的抗肿瘤作用。设计采用台盼蓝排斥法、活/死法和球形法检测BBI608对MC3和jd -15黏液表皮样癌（MEC）细胞株的抗肿瘤活性。通过免疫印迹、4′，6-二氨基-2-苯基吲哚（DAPI）染色、膜联蛋白v -异硫氰酸荧光素/碘化丙啶（V-FITC/PI）双染色和逆转录定量PCR研究凋亡效应。结果bbi608对MEC细胞株有抑制生长作用，降低细胞活力和成球能力，增加细胞死亡。cleaved caspase-3和PARP表达增加，细胞核凋亡形态学改变，Annexin V阳性增加，证实bbi608诱导细胞凋亡。BBI608显著下调Mcl-1表达，促进MEC细胞凋亡。Mcl-1下调似乎分别由MC3和YD-15细胞中的蛋白酶体依赖性蛋白降解和翻译调节机制介导。结论BBI608通过诱导mcl -1依赖性细胞凋亡，有效抑制MEC细胞体外增殖。这表明BBI608作为MEC的潜在治疗剂值得进一步研究。

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引用次数: 0

In vivo immunological evaluation of indomethacin and omega-3 nanocapsules for the treatment of rheumatoid arthritis in the temporomandibular joints of rats 吲哚美辛和omega-3纳米胶囊治疗大鼠颞下颌关节类风湿关节炎的体内免疫学评价

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2026-02-01 Epub Date: 2025-11-11 DOI: 10.1016/j.archoralbio.2025.106456

Victor Augusto Benedicto dos Santos , Francisco Carlos Groppo , Thomas Barbin , Adilson Sartoratto , Luiz Eduardo Nunes Ferreira , Michael Henrique Araujo Monteiro , Sidney Raimundo Figueroba

Objective

This study aimed to develop and characterize indomethacin nanocapsules with an omega-3 oily core and evaluate their potential for treating rheumatoid arthritis in rats, comparing their effects to free-form administration on cytokines IL-1β, IL-10, IL-6, and TNF-α in temporomandibular joint.

Design

Nanocapsules were synthesized with omega-3 as the oil phase containing indomethacin. They were characterized for mean hydrodynamic diameter, polydispersity index, zeta potential, morphology (TEM), encapsulation efficiency (HPLC), and cytotoxicity in RAW 264.7 macrophages (MTT assay). For in vivo evaluation, forty-eight adult male Wistar rats (n = 6) underwent rheumatoid arthritis induction via intradermal injection of Complete Freund's Adjuvant (CFA) and bovine type II collagen (CII) at the base of the tail. Rats were treated for 7 days by oral gavage with either nanocapsules (NC5, NC2.5), free indomethacin (IND5, IND2.5), or indomethacin combined with omega-3 (IND5 +ω3, IND2.5 +ω3). Cytokine levels in the temporomandibular joint were subsequently assessed.

Results

Nanocapsules displayed a spherical, well-defined morphology with diameters < 250 nm and exhibited lower cytotoxicity in RAW 264.7 cells compared to free-form treatments. In vivo, all treated groups showed significant reductions in IL-1β, IL-6, and TNF-α compared to the CFA group, along with a significant increase in IL-10.

Conclusions

Indomethacin nanocapsules with omega-3 effectively reduced pro-inflammatory cytokines and increased anti-inflammatory IL-10, demonstrating a superior immunomodulatory profile compared to free-form treatments.

本研究旨在开发和表征含有omega-3油芯的吲哚美辛纳米胶囊，并评估其治疗大鼠类风湿关节炎的潜力，比较其与自由给药对颞下颌关节细胞因子IL-1β、IL-10、IL-6和TNF-α的影响。设计以omega-3为油相，含吲哚美辛合成纳米胶囊。采用平均水动力直径、多分散性指数、zeta电位、形态（TEM）、包封效率（HPLC）和RAW 264.7巨噬细胞的细胞毒性（MTT法）对其进行表征。为了进行体内评估，48只成年雄性Wistar大鼠（n = 6）通过在尾巴底部皮内注射完全弗氏佐剂（CFA）和牛II型胶原（CII）诱导类风湿关节炎。大鼠分别口服纳米胶囊（NC5、NC2.5）、游离吲哚美辛（IND5、IND2.5）或吲哚美辛联合omega-3 （IND5 +ω3, IND2.5 +ω3）治疗7 d。随后评估了颞下颌关节的细胞因子水平。结果纳米胶囊呈球形，直径为<； 250 nm，在RAW 264.7细胞中表现出较低的细胞毒性。在体内，与CFA组相比，所有治疗组的IL-1β、IL-6和TNF-α水平均显著降低，IL-10水平显著升高。结论含omega-3的辛多美辛纳米胶囊可有效降低促炎细胞因子，增加抗炎IL-10，与自由形式的治疗相比，具有优越的免疫调节作用。

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引用次数: 0

Submandibular saliva from male rats modulates osteogenic differentiation of bone marrow progenitor cells In Vitro 雄性大鼠下颌下唾液对骨髓祖细胞体外成骨分化的调节作用。

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2026-02-01 Epub Date: 2025-12-15 DOI: 10.1016/j.archoralbio.2025.106484

Gastón R. Troncoso , María V. Gangoiti , Javier Fernández-Solari , Claudia E. Mohn , María S. Molinuevo

Objective

To investigate the effect of submandibular saliva from male rats on the proliferation and osteoblastic differentiation of bone marrow progenitor cells.

Design

Saliva was collected and pooled from adult male rats, and the total protein content was measured. Bone marrow progenitor cells (BMPC) from adult male rats were incubated with varying concentrations of salivary protein (ranging from 0 to 30 µg/mL) to assess cell proliferation. Osteoblastic differentiation was evaluated by measuring alkaline phosphatase activity, collagen type 1 production, mineral nodule formation, and molecular markers of differentiation after 7, 15, or 21 days of saliva exposure in a differentiating culture medium. The pro-inflammatory effects of saliva on BMPC were assessed by measuring tumor necrosis factor-alpha, interleukin 1 beta, prostaglandin E2, and matrix metalloproteinases 2 and 9.

Results

Low saliva concentration stimulated BMPC, promoting a pro-secretory and proliferative state. In contrast, higher concentration inhibited both processes under basal conditions. Furthermore, in osteogenic medium, saliva decreased alkaline phosphatase activity, collagen-1 production, and matrix mineralization in BMPC, demonstrating a dose- and time-dependent effect. Saliva also increased the secretion of interleukin 1β, tumor necrosis factor α, prostaglandin E2, and metalloproteinase activity in these cells, which inhibited osteoblastic differentiation.

Conclusion

Submandibular saliva has a biphasic effect on the osteogenic commitment of bone marrow progenitor cells, depending on the concentration and duration of exposure. This finding underscores the active role of saliva in the repair and regeneration of tooth sockets.

目的：探讨雄性大鼠下颌下唾液对骨髓祖细胞增殖和成骨分化的影响。设计：收集成年雄性大鼠唾液，测定总蛋白含量。将成年雄性大鼠骨髓祖细胞（BMPC）与不同浓度的唾液蛋白（0至30 µg/mL）孵育，以评估细胞增殖情况。通过测量碱性磷酸酶活性、1型胶原蛋白生成、矿物结节形成和唾液在分化培养基中暴露7、15或21天后分化的分子标记来评估成骨细胞分化。通过检测肿瘤坏死因子- α、白细胞介素- 1 β、前列腺素E2和基质金属蛋白酶2和9来评估唾液对BMPC的促炎作用。结果：低唾液浓度刺激BMPC，促进分泌和增殖状态。相反，在基础条件下，较高的浓度抑制了这两个过程。此外，在成骨培养基中，唾液降低碱性磷酸酶活性、胶原-1生成和BMPC基质矿化，显示出剂量和时间依赖性效应。唾液还增加了这些细胞中白细胞介素1β、肿瘤坏死因子α、前列腺素E2和金属蛋白酶的分泌，从而抑制成骨细胞的分化。结论：颌下腺唾液对骨髓祖细胞的成骨功能具有双相影响，其影响程度取决于唾液的浓度和暴露时间。这一发现强调了唾液在牙槽修复和再生中的积极作用。

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引用次数: 0

Is there geometric morphometric evidence for the selection against impaction? A comparative cross-sectional study of specific tooth-agenesis patterns 是否有几何形态计量学证据证明选择不受撞击？特定牙齿发育模式的比较横断面研究。

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2026-02-01 Epub Date: 2025-11-15 DOI: 10.1016/j.archoralbio.2025.106462

Sarah Boussali , Anaïs Cavaré

Objectives

Reductions in tooth number and facial prognathism may reflect shared genetic and evolutionary mechanisms but have been inconsistently reported by traditional cephalometric analyses. This study aimed to assess whether common patterns of dental agenesis are associated with craniofacial morphology using geometric morphometric methods.

Design

This retrospective comparative cross-sectional study included 538 patients aged 10–19 years from a French orthodontic population. Individuals with hypodontia (n = 269), restricted to third molars, maxillary lateral incisors, or second premolars, were matched by age and sex with controls without agenesis (n = 269). Craniofacial morphology was assessed on lateral cephalograms using 18 landmarks and 87 semilandmarks. Multiple linear regression and Procrustes MANOVA were applied to evaluate differences in size and shape, adjusting for sex and age.

Results

No significant association was found between agenesis status and craniofacial size in the overall configuration as well as in the cranial base, maxilla, and mandible. A significant sex effect was detected in the mandible, with smaller centroid size in males (p = 0.012). Multivariate regression on shape confirmed allometric effects across all configurations. Procrustes MANOVA detected a significant effect of sex on overall shape (p = 0.003), but neither age nor hypodontia reached significance.

Conclusion

In this large geometric morphometric study, no generalized or localized alterations in craniofacial morphology were detected, even in third molar agenesis, suggesting that the anthropological significance of hypodontia should be regarded with caution.

目的：牙齿数量减少和面部前突可能反映了共同的遗传和进化机制，但传统的头颅测量分析报告不一致。本研究旨在利用几何形态计量学方法评估牙齿发育不全的常见模式是否与颅面形态相关。设计：这项回顾性比较横断面研究包括538名年龄在10-19岁的法国正畸人群。下颌缺失个体（n = 269），局限于第三磨牙，上颌侧门牙或第二前磨牙，按年龄和性别与无发育的对照组（n = 269）匹配。采用18个标志和87个半标志在侧位脑电图上评估颅面形态。应用多元线性回归和Procrustes方差分析评估大小和形状的差异，并对性别和年龄进行调整。结果：发育不全状态与颅面大小、颅底、上颌骨、下颌骨整体形态无显著相关性。下颌骨存在显著的性别效应，男性的质心尺寸较小（p = 0.012）。形状的多元回归证实了所有构型的异速生长效应。Procrustes MANOVA检测到性别对整体形状有显著影响（p = 0.003），但年龄和下颌畸形均无显著影响。结论：在这项大型几何形态测量学研究中，即使在第三磨牙发育中，也没有发现颅面形态的普遍或局部改变，这表明下颌畸形的人类学意义应该谨慎对待。

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引用次数: 0

Tetramethoxyluteolin, an active constituent in mulberry leaves, promotes osteogenesis of jaw bone marrow mesenchymal stem cells in periodontitis microenvironment via NF-κB inhibition 桑叶中的活性成分四甲木犀草素通过抑制NF-κB促进牙周炎微环境下颌骨骨髓间充质干细胞成骨。

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2026-02-01 Epub Date: 2025-11-15 DOI: 10.1016/j.archoralbio.2025.106459

Yuning Xia , Chao Shan , Zeyu Wu , Jin Zhao

Objective

This study aims to investigate the effects and mechanisms of tetramethoxyluteolin (TML), a bioactive compound in mulberry leaves, on jaw bone marrow mesenchymal stem cells (JBMMSCs) in a periodontitis microenvironment.

Design

Network pharmacology and molecular docking were used to identify mulberry leaves' active constituents and their targets in periodontitis treatment. An inflammatory model was established in JBMMSCs using Porphyromonas gingivalis-lipopolysaccharide (5 μg/mL). TML's optimal concentration was determined via CCK-8 and ELISA. Osteogenic differentiation, inflammatory markers, and NF-κB pathway activity were assessed using ALP/ARS staining, RT-PCR, and Western blot (WB). A rat model of ligature-induced periodontitis was established, and TML's effects were evaluated through histopathological staining, micro-CT, RT-PCR, and WB. JBMMSCs from each animal experimental group were isolated for in vitro osteogenic validation. Mechanisms were clarified by comparing TML with the NF-κB inhibitor BAY11–7082.

Results

TML was identified as the key constituent targeting NF-κB and inflammatory mediators (IL-6, IL-1β, TNF-α). 5 μM TML significantly suppressed inflammatory cytokines, promoted osteogenic differentiation, and inhibited NF-κB activation in JBMMSCs. In rats, 30 mg/kg TML markedly reduced inflammation and alveolar bone loss, showing efficacy comparable to indomethacin, and JBMMSCs from TML-treated groups exhibited enhanced osteogenesis. TML's inhibition of NF-κB was similar to BAY11–7082.

Conclusion

TML reduces periodontal inflammation and enhances the osteogenic potential of JBMMSCs by inhibiting the NF-κB pathway, providing a novel strategy for periodontitis-related bone regeneration.

目的：研究桑叶生物活性物质四甲木犀草素（TML）对牙周炎微环境下颌骨骨髓间充质干细胞（JBMMSCs）的影响及其机制。设计：采用网络药理学和分子对接的方法，鉴定桑叶的有效成分及其治疗牙周炎的靶点。采用牙龈卟啉单胞菌脂多糖（5 μg/mL）建立JBMMSCs炎症模型。通过CCK-8和ELISA法确定TML的最佳浓度。采用ALP/ARS染色、RT-PCR和Western blot （WB）检测成骨分化、炎症标志物和NF-κB通路活性。建立大鼠结扎性牙周炎模型，通过组织病理学染色、显微ct、RT-PCR和WB评价TML的作用。从每个动物实验组中分离JBMMSCs进行体外成骨验证。通过比较TML与NF-κB抑制剂BAY11-7082，阐明其作用机制。结果：TML是靶向NF-κB和炎症介质（IL-6、IL-1β、TNF-α）的关键成分。5 μM TML显著抑制JBMMSCs炎症因子，促进成骨分化，抑制NF-κB活化。在大鼠中，30 mg/kg TML显著减少炎症和牙槽骨丢失，其疗效与吲哚美辛相当，TML处理组的JBMMSCs表现出增强的成骨作用。TML对NF-κB的抑制作用与BAY11-7082相似。结论：TML通过抑制NF-κB通路，减轻牙周炎症，增强JBMMSCs成骨潜能，为牙周炎相关骨再生提供了一种新的策略。

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引用次数: 0

Immunohistochemical analysis of immune checkpoint proteins (PD-1, PD-L1 and PD-L2) in giant cell granulomas of the jaws and giant cell tumor of bone 颌骨巨细胞肉芽肿和骨巨细胞瘤中免疫检查点蛋白PD-1、PD-L1和PD-L2的免疫组织化学分析。

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2026-02-01 Epub Date: 2025-11-15 DOI: 10.1016/j.archoralbio.2025.106460

Elton Fernandes Barros , Vanessa Alves de Medeiros , Éricka Janine Dantas da Silveira , João Augusto Vianna Goulart Filho , Pollianna Muniz Alves , Cassiano Francisco Weege Nonaka

Objective

To evaluate the immunoexpression of programmed cell death protein 1 (PD-1) and programmed cell death ligands 1 (PD-L1) and 2 (PD-L2) in giant cell granulomas of the jaws (central giant cell granuloma [CGCG], peripheral giant cell granuloma [PGCG]) and giant cell tumor of bone (GCTB).

Design

Thirty CGCG (15 non-aggressive and 15 aggressive), 15 PGCG, and 15 GCTB were selected. The percentages of cytoplasmic (PD-1, PD-L1, and PD-L2) and nuclear (PD-L1) staining in mononuclear cells (MC) and in non-cannibalistic (ncMGC) and cannibalistic MGC (cMGC) were determined.

Results

Cytoplasmic expression of PD-1 and PD-L1 was observed in all groups, with high median percentages of positivity in ncMGC and cMGC. Compared to CGCG and PGCG, GCTB exhibited higher expression of PD-L1 in MC (p < 0.05). In ncMGC, expression of PD-1 was higher in GCTB compared to non-aggressive CGCG (p < 0.05). Similarly, higher PD-L1 immunopositivity was found in ncMGC of GCTB compared to aggressive CGCG and PGCG (p < 0.05). For all cell types, lower median percentages of PD-L2 positivity were observed in GCTB compared to CGCG and PGCG (p > 0.05). In GCTB, there was a strong positive correlation between the cytoplasmic expression of PD-L1 in MC and PD-1 in ncMGC (r = 0.535; p < 0.05). All groups exhibited low nuclear immunoexpression of PD-L1.

Conclusion

The results suggest the potential participation of PD-1, PD-L1, and PD-L2 in the pathogenesis of CGCG, PGCG, and GCTB. The locally aggressive behavior of GCTB could be associated with a higher osteoclastogenic and immunosuppressive microenvironment in these neoplasms.

目的：探讨程序性细胞死亡蛋白1 （PD-1）和程序性细胞死亡配体1 （PD-L1）、2 （PD-L2）在颌骨巨细胞肉芽肿（中央巨细胞肉芽肿[CGCG]、外周巨细胞肉芽肿[PGCG]）和骨巨细胞瘤（GCTB）中的免疫表达。设计：选择30例CGCG（非侵袭性15例，侵袭性15例），PGCG 15例，GCTB 15例。测定单核细胞（MC）、非同类相食（ncMGC）和同类相食MGC （cMGC）细胞质（PD-1、PD-L1和PD-L2）和核（PD-L1）染色百分比。结果：PD-1和PD-L1在所有组的细胞质中均有表达，ncMGC和cMGC的中位阳性百分比较高。与CGCG和PGCG相比，GCTB在MC中的PD-L1表达更高（p  0.05）。在GCTB中，MC细胞质中PD-L1的表达与ncMGC中PD-1的表达呈正相关（r = 0.535;p ）。结论：提示PD-1、PD-L1和PD-L2可能参与了CGCG、PGCG和GCTB的发病过程。GCTB的局部侵袭行为可能与这些肿瘤中较高的破骨细胞生成和免疫抑制微环境有关。

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引用次数: 0

Effects of Bifidobacterium longum, Lactobacillus rhamnosus, and Lactobacillus reuteri on oral microbial balance and host cell functions: Implications for the prevention and management of oral diseases 长双歧杆菌、鼠李糖乳杆菌和罗伊氏乳杆菌对口腔微生物平衡和宿主细胞功能的影响：对口腔疾病预防和管理的影响

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2026-02-01 Epub Date: 2025-11-11 DOI: 10.1016/j.archoralbio.2025.106458

Zhigang Zhu , Xiang Li , Jianwei Liu , Liang Chen , Yutong Zhan , Li Wang , Luxian Zhou , Dandan Jiang , Xianwu Peng , Xiuyu Jiang

Objectives

This study aimed to evaluate the effects of three probiotic samples—Bifidobacterium longum, Lactobacillus rhamnosus, and Lactobacillus reuteri—on oral pathogens, commensal bacteria, and host oral cells, thereby exploring their potential roles in maintaining microbial balance and promoting host defense.

Design

Probiotic culture supernatants (postbiotic fractions) were tested against oral pathogens (S. mutans, P. gingivalis, F. nucleatum, A. actinomycetemcomitans) and commensal species (S. sanguinis, V. parvula) using optical density assays. Primary human gingival fibroblasts and oral keratinocytes were co-cultured with probiotic preparations to assess cell viability, inflammatory cytokine secretion, and barrier gene expression (FLG, LOR).

Results

B. longum, L. rhamnosus, and L. reuteri samples showed strain- and concentration-dependent inhibition of oral pathogens, while exhibiting minimal effects on beneficial commensals. Appropriate concentrations of probiotic samples preserved fibroblast viability and enhanced keratinocyte survival. Notably, B. longum, L. rhamnosus, and L. reuteri upregulated barrier-associated genes (FLG, LOR) and suppressed IL-6 secretion in inflamed keratinocytes, suggesting immunomodulatory and protective roles.

Conclusions

Probiotic-derived metabolites exert selective antimicrobial and cytoprotective effects in the oral microenvironment. These findings support the dual functions of probiotics in reshaping oral microbial balance and modulating host cell functions and highlight the potential for personalized probiotic strategies in oral disease prevention and management.Further exploration of combined probiotic formulations is warranted to optimize clinical translation.

目的研究长双歧杆菌、鼠李糖乳杆菌和罗伊氏乳杆菌三种益生菌样品对口腔病原菌、共生菌和宿主口腔细胞的影响，探讨其在维持微生物平衡和促进宿主防御方面的潜在作用。设计采用光密度法检测益生菌培养上清液（生后组分）对口腔病原菌（变形链球菌、牙龈卟啉卟啉链球菌、核核卟啉卟啉卟啉链球菌、放线菌卟啉卟啉链球菌）和共生菌（血源性卟啉链球菌、细小弧菌）的抑制作用。用益生菌制剂对原代人牙龈成纤维细胞和口腔角化细胞进行共培养，观察细胞活力、炎性细胞因子分泌和屏障基因表达（FLG， LOR）。龙舌兰、鼠李糖乳杆菌和罗伊氏乳杆菌样品显示出菌株和浓度依赖性的口腔病原体抑制，而对有益共生菌的影响最小。适当浓度的益生菌样品可以保存成纤维细胞的活力，提高角质细胞的存活率。值得注意的是，长芽孢杆菌、鼠李糖乳杆菌和罗伊氏乳杆菌上调了屏障相关基因（FLG、LOR），抑制了炎症角质形成细胞中IL-6的分泌，提示其具有免疫调节和保护作用。结论益生菌衍生代谢物在口腔微环境中具有选择性抗菌和细胞保护作用。这些发现支持了益生菌在重塑口腔微生物平衡和调节宿主细胞功能方面的双重功能，并强调了个性化益生菌在口腔疾病预防和管理中的潜力。进一步探索联合益生菌制剂是必要的，以优化临床转化。

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