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Evaluation of the bioelectrical activity of the masticatory muscles in patients with narrowed maxillary transverse dimension compared to the occlusal norm 与咬合正常值相比,评估上颌横径变窄患者咀嚼肌的生物电活性
IF 2.2 4区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-07-11 DOI: 10.1016/j.archoralbio.2024.106049

Objective

The aim of the study was to determine how the electrical activity of the temporalis, masseter and sternocleidomastoid muscles differs in children with reduced transverse jaw dimension compared to children with normal occlusion. Design: It was a experimental study. Thirty-seven patients were included in the study. 18 in the study group received orthodontic treatment with removable appliances and 19 subjects were classified as normal occlusion subjects in the control group. A panoramic X-ray and digital intraoral scan were taken, followed by an surface electromyography of three muscle pairs (temporalis muscles, masseter muscles, sternocleidomastoid muscles) in resting position, while clenching and clenching on cotton rollers. Results: There was significantly greater activity in the experimental group than in the control group comparing muscles: temporalis muscles and masseter muscles in the resting position. Additionally, significantly greater activity of muscles in the control group was found during clenching. However, the asymmetry index of muscles indicates that there is significantly greater asymmetry of muscles activity in the experimental group. Compared to children with normal occlusion, children with a narrowed transverse dimension of the jaw have statistically significant differences in the bioelectrical activity of the temporalis, masseter and sternocleidomastoid muscles, as well as greater asymmetry in the bioelectrical voltage of the masseter muscles. Conclusions: Patients with reduced transverse dimension of the jaw are characterized by increased resting activity of the masticatory muscles and reduced functional activity of the masticatory muscles. These patients have increased asymmetry in the bioelectrical tension of the masticatory muscles.

目的 该研究旨在确定与咬合正常的儿童相比,下颌横向尺寸缩小的儿童的颞肌、咀嚼肌和胸锁乳突肌的电活动有何不同。设计这是一项实验研究。研究共纳入 37 名患者。研究组中有 18 名患者接受了活动矫治器的正畸治疗,对照组中有 19 名咬合正常的患者。研究人员拍摄了全景 X 光片和数字口腔内扫描,然后对三对肌肉(颞肌、颌面肌、胸锁乳突肌)进行了表面肌电图检查,包括静止状态下的肌电图、咬紧时的肌电图和在棉卷上咬紧时的肌电图。结果显示与对照组相比,实验组颞肌和斜方肌在静止姿势下的肌肉活动明显更活跃。此外,对照组肌肉在紧握时的活动度也明显高于实验组。然而,肌肉的不对称指数表明,实验组的肌肉活动明显更不对称。与咬合正常的儿童相比,下颌横向尺寸狭窄的儿童在颞肌、颌下肌和胸锁乳突肌的生物电活动方面存在统计学意义上的显著差异,而且颌下肌生物电电压的不对称性更大。结论下颌横向尺寸减小的患者的特点是咀嚼肌的静息活动增加,而咀嚼肌的功能活动减少。这些患者咀嚼肌生物电张力的不对称性增加。
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引用次数: 0
Naltrexone accelerated oral traumatic ulcer healing and downregulated TLR-4/NF-kB pathway in Wistar rats 纳曲酮加速 Wistar 大鼠口腔创伤溃疡愈合并下调 TLR-4/NF-kB 通路
IF 2.2 4区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-07-07 DOI: 10.1016/j.archoralbio.2024.106047

Objective

To assess the effect of naltrexone on oral mucosal healing using a traumatic ulcer model

Design

Wistar rats (n = 112) received distilled water (control) or naltrexone (0.5, 10, or 50 mg/kg/day). Ulcers were induced on the buccal mucosa using a round skin biopsy punch (diameter 6 mm). Euthanasia was performed on days 1, 3, 7, and 14. Healing was assessed by ulcer area, histological scores, histomorphometric analysis (number of polymorphonuclears, mononuclears, and fibroblasts), and collagen percentage. Immunohistochemistry for TLR-2, TLR-4, NF-kB, and CD31 was evaluated. Nociceptive threshold was measured daily.

Results

The 50 mg/kg group showed reduced ulcer area on days 1 (p < 0.001), 3 (p < 0.05), and 14 (p < 0.01). In this group, there was, on day 14, an increase in the percentage of reepithelization (p = 0.043) and collagen (p < 0.05), an increase in connective tissue maturation (p = 0.016), and on day 7 an increase in fibroblasts (p < 0.001). The 10 mg/kg dose reduced the ulcer area on day 1 (p < 0.001). The 50 mg/kg group showed lower expression of TLR-4 (p < 0.001) on day 1, NF-kB on days 1 (p < 0.05) and 14 (p < 0.05), and CD31 on day 14 (p < 0.05). The 0.5 and 10 mg/kg doses reduced TLR-4 expression on day 1 (p < 0.05; p < 0.01, respectively). Nociceptive threshold increased in the 50 mg/kg group (p < 0.01).

Conclusion

Naltrexone enhanced traumatic oral ulcer healing by reducing TLR-4/NF-kB signaling and promoting fibroblast proliferation and collagen deposition. Additionally, naltrexone reduced pain in rats.

目的利用外伤性溃疡模型评估纳曲酮对口腔黏膜愈合的影响。设计将蒸馏水(对照组)或纳曲酮(0.5、10 或 50 毫克/千克/天)浸泡大鼠(112 只)。使用圆形皮肤活检打孔器(直径 6 毫米)在大鼠口腔粘膜上诱发溃疡。在第 1、3、7 和 14 天实施安乐死。通过溃疡面积、组织学评分、组织形态计量分析(多形核、单核细胞和成纤维细胞的数量)和胶原蛋白百分比来评估愈合情况。对 TLR-2、TLR-4、NF-kB 和 CD31 进行了免疫组化评估。结果 50 mg/kg 组在第 1 天(p < 0.001)、第 3 天(p < 0.05)和第 14 天(p < 0.01)溃疡面积缩小。在该组中,第 14 天,再上皮(p = 0.043)和胶原蛋白(p <;0.05)的比例增加,结缔组织成熟度增加(p = 0.016),第 7 天,成纤维细胞增加(p <;0.001)。10 毫克/千克剂量可减少第 1 天的溃疡面积(p < 0.001)。50 毫克/千克组在第 1 天的 TLR-4 表达较低(p < 0.001),第 1 天和第 14 天的 NF-kB 表达较低(p < 0.05),第 14 天的 CD31 表达较低(p < 0.05)。0.5 毫克/千克和 10 毫克/千克剂量可减少第 1 天 TLR-4 的表达(分别为 p < 0.05;p < 0.01)。结论 纳曲酮通过减少TLR-4/NF-kB信号传导、促进成纤维细胞增殖和胶原蛋白沉积,促进创伤性口腔溃疡愈合。此外,纳曲酮还能减轻大鼠的疼痛。
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引用次数: 0
Brusatol improves the efficacy of an anti-mouse-PD-1 antibody via inhibiting programmed cell death 1 ligand 1 expression in a murine head and neck squamous cell carcinoma model 在小鼠头颈部鳞状细胞癌模型中,布卢沙托通过抑制程序性细胞死亡 1 配体 1 的表达提高了抗小鼠-PD-1 抗体的疗效。
IF 2.2 4区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-07-03 DOI: 10.1016/j.archoralbio.2024.106043
Yanlin Wu , Guilian Zhang , Panpan Yin , Jinlin Wen , Ying Su , Xinyan Zhang

Objective

Combing PD-1/PD-L1 immune checkpoint inhibitors with natural products has exhibited better efficacy than monotherapy. Hence, the purpose of this research was to examine the anti-cancer effects of brusatol, a natural quassinoid-terpenoid derived from Brucea javanica, when used in conjunction with an anti-mouse-PD-1 antibody in a murine head and neck squamous cell carcinoma (HNSCC) model and elucidate underlying mechanisms.

Design

A murine HNSCC model and an SCC-15 cell xenograft nude mouse model were established to investigate the anti-cancer effects of brusatol and anti-PD-1 antibody. Mechanistic studies were performed using immunohistochemistry. Cell proliferation, migration, colony formation, and invasion were evaluated by MTT, migration, colony formation, and transwell invasion assays. PD-L1 levels in oral squamous cell carcinoma (OSCC) cells were assessed through qRT-PCR, flow cytometry, and western blotting assays. The impact of brusatol on Jurkat T cell function was assessed by an OSCC/Jurkat co-culture assay.

Results

Brusatol improved tumor suppression by anti-PD-1 antibody in HNSCC mouse models. Mechanistic studies revealed brusatol inhibited tumor cell growth and angiogenesis, induced apoptosis, increased T lymphocyte infiltration, and reduced PD-L1 expression in tumors. Furthermore, in vitro assays confirmed brusatol inhibited PD-L1 expression in OSCC cells and suppressed cell migration, colony formation, and invasion. Co-culture assays indicated that brusatol's PD-L1 inhibition enhanced Jurkat T cell-mediated OSCC cell death and reversed the inhibitory effect induced by OSCC cells.

Conclusions

Brusatol improves anti-PD-1 antibody efficacy by targeting PD-L1, suggesting its potential as an adjuvant in anti-PD-1 immunotherapy.

目的:与单一疗法相比,将 PD-1/PD-L1 免疫检查点抑制剂与天然产品结合使用具有更好的疗效。因此,本研究的目的是在小鼠头颈部鳞状细胞癌(HNSCC)模型中检测布鲁斯特醇(一种提取自布鲁斯特的天然槲皮素-三萜类化合物)与抗小鼠 PD-1 抗体联合使用的抗癌效果,并阐明其潜在机制:设计:建立小鼠 HNSCC 模型和 SCC-15 细胞异种移植裸鼠模型,研究布芦沙托和抗 PD-1 抗体的抗癌作用。采用免疫组化方法进行机理研究。细胞增殖、迁移、集落形成和侵袭通过 MTT、迁移、集落形成和 transwell 侵袭试验进行评估。口腔鳞状细胞癌(OSCC)细胞中的 PD-L1 水平通过 qRT-PCR、流式细胞术和 Western 印迹法进行评估。通过OSCC/Jurkat共培养试验评估了布芦沙托对Jurkat T细胞功能的影响:结果:在HNSCC小鼠模型中,布芦沙托能改善抗PD-1抗体对肿瘤的抑制作用。机理研究表明,布芦沙托能抑制肿瘤细胞生长和血管生成,诱导细胞凋亡,增加T淋巴细胞浸润,减少肿瘤中PD-L1的表达。此外,体外实验证实,布芦沙托能抑制 OSCC 细胞中 PD-L1 的表达,抑制细胞迁移、集落形成和侵袭。共培养试验表明,布芦沙托对PD-L1的抑制增强了Jurkat T细胞介导的OSCC细胞死亡,并逆转了OSCC细胞诱导的抑制作用:结论:布鲁沙托通过靶向PD-L1提高了抗PD-1抗体的疗效,这表明布鲁沙托具有作为抗PD-1免疫疗法辅助剂的潜力。
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引用次数: 0
Correlation of disulfidptosis and periodontitis: New insights and clinical significance 二硫化血症与牙周炎的相关性:新见解和临床意义。
IF 2.2 4区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-07-02 DOI: 10.1016/j.archoralbio.2024.106046
Yixin Fan , Wantong Liu , Le Qi , Qi Zhao , Sining Li , He Zou , Chen Kong , Zhiwei Li , Jiwei Ren , Zhihui Liu , Bowei Wang

Objectives

This study aims to investigate and predict the therapeutic agents associated with disulfidptosis in periodontitis.

Design

The dataset GSE10334 was downloaded from the Gene Expression Omnibus (GEO) database and used to train a least absolute shrinkage and selection operator (LASSO) regression and support vector machine recursive feature elimination (SVM–RFE) algorithm to identify genes associated with disulfidptosis in periodontitis. GSE16134 validation sets, polymerase chain reaction (PCR), and gingival immunofluorescence were used to verify the results.Single-gene Gene Set Enrichment Analysis (GSEA) was performed to explore the potential mechanisms and functions of the characterized genes. Immune infiltration and correlation analyses were performed, and competing endogenous RNA (ceRNA) networks were constructed. Effective therapeutic drugs were then predicted using the DGIdb database, and molecular docking was used to validate binding affinity.

Results

Six genes (SLC7A11, SLC3A2, RPN1, NCKAP1, LRPPRC, and NDUFS1) associated with disulfidptosis in periodontitis were obtained. Validation results from external datasets and experiments were consistent with the screening results. Single-gene GSEA analysis was mainly enriched for antigen presentation and immune-related pathways and functions.Immune infiltration and correlation analyses revealed significant regulatory relationships between these genes and plasma cells, resting dendritic cell, and activated NK cells. The ceRNA network was visualized. And ME-344, NV-128, and RILUZOLE, which have good affinity to target genes, were identified as promising agents for the treatment of periodontitis.

Conclusions

SLC7A11, SLC3A2, RPN1, NCKAP1, LRPPRC, and NDUFS1 are targets associated with disulfidptosis in periodontitis, and ME-344, NV-128, and RILUZOLE are promising agents for the treatment of periodontitis.

研究目的本研究旨在调查和预测与牙周炎二硫化相关的治疗药物:设计:从基因表达总库(GEO)数据库下载数据集GSE10334,用于训练最小绝对收缩和选择算子(LASSO)回归和支持向量机递归特征消除(SVM-RFE)算法,以识别牙周炎中与二硫化硫相关的基因。为验证结果,研究人员使用了GSE16134验证集、聚合酶链反应(PCR)和牙龈免疫荧光。进行了免疫浸润和相关性分析,并构建了竞争性内源性 RNA(ceRNA)网络。然后利用 DGIdb 数据库预测了有效的治疗药物,并利用分子对接验证了结合亲和力:结果:获得了与牙周炎二硫化相关的六个基因(SLC7A11、SLC3A2、RPN1、NCKAP1、LRPPRC 和 NDUFS1)。外部数据集和实验的验证结果与筛选结果一致。单基因GSEA分析主要富集于抗原提呈和免疫相关通路和功能。免疫浸润和相关性分析表明,这些基因与浆细胞、静止树突状细胞和活化NK细胞之间存在显著的调控关系。对 ceRNA 网络进行了可视化分析。与靶基因具有良好亲和力的 ME-344、NV-128 和 RILUZOLE 被确定为治疗牙周炎的有前途的药物:结论:SLC7A11、SLC3A2、RPN1、NCKAP1、LRPPRC 和 NDUFS1 是牙周炎中与二硫化相关的靶基因,ME-344、NV-128 和 RILUZOLE 是治疗牙周炎的有效药物。
{"title":"Correlation of disulfidptosis and periodontitis: New insights and clinical significance","authors":"Yixin Fan ,&nbsp;Wantong Liu ,&nbsp;Le Qi ,&nbsp;Qi Zhao ,&nbsp;Sining Li ,&nbsp;He Zou ,&nbsp;Chen Kong ,&nbsp;Zhiwei Li ,&nbsp;Jiwei Ren ,&nbsp;Zhihui Liu ,&nbsp;Bowei Wang","doi":"10.1016/j.archoralbio.2024.106046","DOIUrl":"10.1016/j.archoralbio.2024.106046","url":null,"abstract":"<div><h3>Objectives</h3><p>This study aims to investigate and predict the therapeutic agents associated with disulfidptosis in periodontitis.</p></div><div><h3>Design</h3><p>The dataset GSE10334 was downloaded from the Gene Expression Omnibus (GEO) database and used to train a least absolute shrinkage and selection operator (LASSO) regression and support vector machine recursive feature elimination (SVM–RFE) algorithm to identify genes associated with disulfidptosis in periodontitis. GSE16134 validation sets, polymerase chain reaction (PCR), and gingival immunofluorescence were used to verify the results.Single-gene Gene Set Enrichment Analysis (GSEA) was performed to explore the potential mechanisms and functions of the characterized genes. Immune infiltration and correlation analyses were performed, and competing endogenous RNA (ceRNA) networks were constructed. Effective therapeutic drugs were then predicted using the DGIdb database, and molecular docking was used to validate binding affinity.</p></div><div><h3>Results</h3><p>Six genes (SLC7A11, SLC3A2, RPN1, NCKAP1, LRPPRC, and NDUFS1) associated with disulfidptosis in periodontitis were obtained. Validation results from external datasets and experiments were consistent with the screening results. Single-gene GSEA analysis was mainly enriched for antigen presentation and immune-related pathways and functions.Immune infiltration and correlation analyses revealed significant regulatory relationships between these genes and plasma cells, resting dendritic cell, and activated NK cells. The ceRNA network was visualized. And ME-344, NV-128, and RILUZOLE, which have good affinity to target genes, were identified as promising agents for the treatment of periodontitis.</p></div><div><h3>Conclusions</h3><p>SLC7A11, SLC3A2, RPN1, NCKAP1, LRPPRC, and NDUFS1 are targets associated with disulfidptosis in periodontitis, and ME-344, NV-128, and RILUZOLE are promising agents for the treatment of periodontitis.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141592414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Expression of wound healing markers in gingival crevicular fluid following root-coverage procedures: A systematic review of randomized clinical trials 牙根覆盖术后牙龈缝液中伤口愈合标记物的表达:随机临床试验的系统回顾。
IF 2.2 4区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-07-01 DOI: 10.1016/j.archoralbio.2024.106035
Vikender Singh Yadav , Kanika Makker , Nitesh Tewari , Nitika Monga , Rajiv Balachandran , Ujjal Kumar Bhawal , Ajay Mahajan

Objective

Although several surgical techniques have been developed for treatment of gingival recession (GR), the underlying wound healing process remains relatively unexplored. This systematic review aimed to investigate the expression of wound healing markers in gingival crevicular fluid (GCF) before and after surgical treatment of GR.

Design

Randomized clinical trials (RCTs) reporting changes in the expression of GCF markers following any root coverage surgical procedure were identified from 4 electronic databases and manual searches followed by data extraction and result synthesis. The risk of bias (RoB) was assessed using Cochrane RoB 2.0 tool. Overall certainty of evidence was summarized using the Grading of Recommendations Assessment, Development and Evaluation (GRADE) tool.

Results

Four RCTs comprising 100 patients and investigating 15 biomarkers were included. Post-surgery, GCF levels of cytokines and inflammatory proteins were raised during the first 2–10 days of healing. MMP-8 levels increased during the first week followed by a gradual decline. RoB was found to be high for all studies and the overall certainty of evidence was very low.

Conclusion

A limited number of studies with large methodological variations precluded reliable conclusions. Well-designed studies powered for GCF markers’ levels that follow a standardized protocol for GCF sampling and processing are needed to draw conclusive evidence.

目的:尽管目前已开发出多种手术技术来治疗牙龈退缩(GR),但伤口愈合的基本过程仍相对缺乏研究。本系统综述旨在研究手术治疗牙龈退缩前后龈沟液(GCF)中伤口愈合标记物的表达:设计:从 4 个电子数据库中找到了报告任何牙根覆盖手术后 GCF 标记表达变化的随机临床试验 (RCT),并进行人工检索,然后提取数据并进行结果综合。使用 Cochrane RoB 2.0 工具评估偏倚风险(RoB)。采用建议评估、发展和评价分级(GRADE)工具对证据的总体确定性进行总结:结果:共纳入了四项研究,包括 100 名患者和 15 个生物标志物。手术后,细胞因子和炎症蛋白的 GCF 水平在愈合的最初 2-10 天内升高。MMP-8 水平在第一周上升,随后逐渐下降。所有研究的RoB都很高,证据的总体确定性很低:结论:研究数量有限,方法差异较大,因此无法得出可靠的结论。要想获得确凿证据,需要进行设计良好的研究,对 GCF 标记物的水平进行检测,并遵循标准化的 GCF 采样和处理方案。
{"title":"Expression of wound healing markers in gingival crevicular fluid following root-coverage procedures: A systematic review of randomized clinical trials","authors":"Vikender Singh Yadav ,&nbsp;Kanika Makker ,&nbsp;Nitesh Tewari ,&nbsp;Nitika Monga ,&nbsp;Rajiv Balachandran ,&nbsp;Ujjal Kumar Bhawal ,&nbsp;Ajay Mahajan","doi":"10.1016/j.archoralbio.2024.106035","DOIUrl":"10.1016/j.archoralbio.2024.106035","url":null,"abstract":"<div><h3>Objective</h3><p>Although several surgical techniques have been developed for treatment of gingival recession (GR), the underlying wound healing process remains relatively unexplored. This systematic review aimed to investigate the expression of wound healing markers in gingival crevicular fluid (GCF) before and after surgical treatment of GR.</p></div><div><h3>Design</h3><p>Randomized clinical trials (RCTs) reporting changes in the expression of GCF markers following any root coverage surgical procedure were identified from 4 electronic databases and manual searches followed by data extraction and result synthesis. The risk of bias (RoB) was assessed using Cochrane RoB 2.0 tool. Overall certainty of evidence was summarized using the Grading of Recommendations Assessment, Development and Evaluation (GRADE) tool.</p></div><div><h3>Results</h3><p>Four RCTs comprising 100 patients and investigating 15 biomarkers were included. Post-surgery, GCF levels of cytokines and inflammatory proteins were raised during the first 2–10 days of healing. MMP-8 levels increased during the first week followed by a gradual decline. RoB was found to be high for all studies and the overall certainty of evidence was very low.</p></div><div><h3>Conclusion</h3><p>A limited number of studies with large methodological variations precluded reliable conclusions. Well-designed studies powered for GCF markers’ levels that follow a standardized protocol for GCF sampling and processing are needed to draw conclusive evidence.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141604633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
M2 macrophage-derived exosomes for bone regeneration: A systematic review 用于骨再生的 M2 巨噬细胞衍生外泌体:系统综述。
IF 2.2 4区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-06-27 DOI: 10.1016/j.archoralbio.2024.106034
Alireza Daneshvar , Parisa Nemati , Ali Azadi , Reza Amid , Mahdi Kadkhodazadeh

Objective

This systematic review aims to evaluate existing evidence to investigate the therapeutic efficacy of M2 macrophage-derived exosomes in bone regeneration.

Design

A comprehensive search between 2020 and 2024 across PubMed, Web of Science, and Scopus was conducted using a defined search strategy to identify relevant studies regarding the following question: “What is the impact of M2 macrophage-derived exosomes on bone regeneration?”. Controlled in vitro and in vivo studies were included in this study. The SYRCLE tool was used to evaluate the risk of bias in the included animal studies.

Results

This review included 20 studies published. Seven studies were selected for only in vitro analysis, whereas 13 studies underwent both in vitro and in vivo analyses. The in vivo studies employed animal models, including 163 C57BL6 mice and 73 Sprague-Dawley rats. Exosomes derived from M2 macrophages were discovered to be efficacious in promoting bone regeneration and vascularization in animal models of bone defects. These effects were primarily confirmed through morphological and histological assessments. This remarkable outcome is attributed to the regulation of multiple signaling pathways, as evidenced by the findings of 11 studies investigating the involvement of miRNAs in this intricate process. In addition, in vitro studies observed positive effects on cell proliferation, migration, osteogenesis, and angiogenesis. Heterogeneity in study methods hinders direct comparison of results across studies.

Conclusion

M2 macrophage-derived exosomes demonstrate remarkable potential for promoting bone regeneration. Further research optimizing their application and elucidating the underlying mechanisms can pave the way for clinical translation.

目的本系统综述旨在评估现有证据,研究M2巨噬细胞衍生的外泌体在骨再生中的疗效:设计:采用确定的检索策略,在2020年至2024年期间对PubMed、Web of Science和Scopus进行了全面检索,以确定与以下问题相关的研究:"M2巨噬细胞衍生的外泌体对骨再生有何影响?本研究纳入了受控的体外和体内研究。采用SYRCLE工具对纳入的动物研究进行偏倚风险评估:结果:本综述包括 20 项已发表的研究。其中 7 项研究只进行了体外分析,13 项研究同时进行了体外和体内分析。体内研究采用的动物模型包括 163 只 C57BL6 小鼠和 73 只 Sprague-Dawley 大鼠。研究发现,源自 M2 巨噬细胞的外泌体可有效促进骨缺损动物模型的骨再生和血管化。这些效果主要通过形态学和组织学评估得到证实。11 项研究发现,miRNAs 参与了这一错综复杂的过程。此外,体外研究还观察到了对细胞增殖、迁移、成骨和血管生成的积极影响。研究方法的异质性阻碍了对不同研究结果的直接比较:结论:M2巨噬细胞衍生的外泌体在促进骨再生方面具有显著的潜力。结论:M2巨噬细胞衍生的外泌体在促进骨再生方面表现出了非凡的潜力,进一步研究优化其应用并阐明其潜在机制可为临床转化铺平道路。
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引用次数: 0
The association between genetic factors and temporomandibular disorders: A systematic literature review 遗传因素与颞下颌关节紊乱之间的关联:系统性文献综述。
IF 2.2 4区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-06-19 DOI: 10.1016/j.archoralbio.2024.106032
Ahid Amer Alshahrani , Ravinder S. Saini , Abdulmajeed Okshah , Abdulkhaliq Ali F. Alshadidi , Masroor Ahmed Kanji , Rajesh Vyas , Rayan Ibrahim H. Binduhayyim , Naseer Ahmed , Seyed Ali Mosaddad , Artak Heboyan

Objective

This study aimed to investigate the correlation between genetic factors and the occurrence and progression of temporomandibular disorders (TMDs) using a comprehensive review and meta-analysis.

Design

A comprehensive search was conducted using the ScienceDirect, PubMed, Cochrane Library, Dimensions, and Emerald databases. A reviewer selected the study using modified PICO criteria, considering human subjects with TMDs, comparing different genetic factors among TMD and non-TMD patients, and reporting TMD signs and symptoms as outcomes. The methodological standards of the eligible papers were assessed using the Joanna Briggs Institute (JBI) Critical Appraisal Checklist for Non-randomized Experimental Investigations. Information was collected methodically and examined.

Results

The electronic database search yielded 851 articles, 19 of which were included in this study. The data analysis showed a significant influence of genetic factors, such as polymorphisms and gene differences, on the development of TMD signs and symptoms, such as myofascial pain, chronic pain, and disc displacement. In addition, gene polymorphism significantly influenced TMD development, with an odds ratio of 2.46 (1.93–3.14) and p of 0.00001.

Conclusions

Genetic factors significantly influenced TMD signs and symptoms, and genetic polymorphisms significantly influenced TMD onset and progression. Further research should be conducted in diverse settings with larger sample sizes to verify and validate these findings.

研究目的本研究旨在通过综合综述和荟萃分析,探讨遗传因素与颞下颌关节紊乱症(TMD)的发生和发展之间的相关性:设计:使用 ScienceDirect、PubMed、Cochrane Library、Dimensions 和 Emerald 数据库进行了全面检索。审稿人采用修改后的 PICO 标准筛选研究,考虑以患有 TMD 的人为研究对象,比较 TMD 患者和非 TMD 患者的不同遗传因素,并将 TMD 征兆和症状作为研究结果进行报告。采用乔安娜-布里格斯研究所(Joanna Briggs Institute,JBI)的《非随机实验研究批判性评估核对表》对符合条件的论文进行了方法学标准评估。收集信息的方法和检查结果:通过电子数据库搜索,共获得 851 篇文章,其中 19 篇被纳入本研究。数据分析显示,多态性和基因差异等遗传因素对 TMD 体征和症状(如肌筋膜疼痛、慢性疼痛和椎间盘移位)的形成有显著影响。此外,基因多态性对 TMD 的发展也有显著影响,其几率比为 2.46(1.93-3.14),P 值为 0.00001:遗传因素对 TMD 的体征和症状有明显影响,基因多态性对 TMD 的发病和发展有明显影响。进一步的研究应在不同的环境中以更大的样本量进行,以核实和验证这些发现。
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引用次数: 0
Oral behaviors and anxiety are significant predictors of jaw function limitation in patients with anterior disc displacement without reduction 口腔行为和焦虑是椎间盘前移位患者下颌功能受限的重要预测因素。
IF 2.2 4区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-06-18 DOI: 10.1016/j.archoralbio.2024.106033
Zhong-yi Fang , Yang Yang , Yuan Yao, Sha-sha Liu, Li-kun Liu, Shen-ji Lu, Hong Zeng, Bin Cai, Li-li Xu

Objective

We aimed to describe jaw function characteristics in patients with anterior disc displacement without reduction (ADDWoR) using the jaw function limitation scale (JFLS), and to investigate the effects of biopsychosocial risk factors on limited jaw function.

Design

In this cross-sectional study of 636 patients with ADDWoR (females, 568; males, 68), we used the JFLS to assess jaw function. Behavioral, psychological, sociodemographic, and biomedical data were collected. Multivariate logistic regression analysis was used to determine risk factors affecting limited jaw function. A receiver operating characteristic curve was used to evaluate the predictive effect of these risk factors.

Results

ADDWoR-associated limitations included restricted jaw mobility and mastication, which exceeded median global functional limitations scale scores, especially mouth opening to bite an apple and chewing tough food. Females had greater limitations in jaw mobility, verbal and emotional communication, and overall. Multivariate logistic regression analysis findings indicated that oral behaviors, anxiety, sex, pain intensity, and maximal mouth opening (MMO) were predictive of limited jaw function (area under the curve, 72 %).

Conclusion

Patients with ADDWoR reported mastication and jaw mobility restrictions, with females having more pronounced limitations, and specific risk factors identified as significant predictors of jaw function limitations. Along with pain relief and improvement in MMO, appropriate psychological counseling and oral behavioral correction facilitates recovery of jaw function in such patients.

目的我们旨在使用颌骨功能限制量表(JFLS)描述椎间盘前移位(ADDWoR)患者的颌骨功能特征,并研究生物心理社会风险因素对颌骨功能受限的影响:在这项对 636 名 ADDWoR 患者(女性 568 名;男性 68 名)进行的横断面研究中,我们使用 JFLS 对颌骨功能进行了评估。我们还收集了行为、心理、社会人口学和生物医学数据。多变量逻辑回归分析用于确定影响下颌功能受限的风险因素。使用接收器操作特征曲线评估这些风险因素的预测效果:与ADDWoR相关的限制包括下颌活动度和咀嚼受限,这超过了全球功能限制量表的中位数分数,尤其是张嘴咬苹果和咀嚼坚硬食物。女性在颌骨活动度、言语和情感交流以及整体方面的限制更大。多变量逻辑回归分析结果表明,口腔行为、焦虑、性别、疼痛强度和最大张口度(MMO)对颌骨功能受限具有预测作用(曲线下面积为 72 %):ADDWoR患者的咀嚼和下颌活动受限,女性受限更明显,特定的风险因素被认为是下颌功能受限的重要预测因素。在缓解疼痛和改善咀嚼活动度的同时,适当的心理辅导和口腔行为矫正有助于这类患者恢复颌骨功能。
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引用次数: 0
Acquired pellicle and biofilm engineering with CaneCPI-5: Insights from proteomic and microbiomics analysis 利用 CaneCPI-5 进行后天细胞膜和生物膜工程:蛋白质组学和微生物组学分析的启示。
IF 2.2 4区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-06-18 DOI: 10.1016/j.archoralbio.2024.106025
Tamara Teodoro Araujo , Aline Dionizio , Thamyris de Souza Carvalho , Ana Luiza Bogaz Debortolli , Mariele Vertuan , Beatriz Martines de Souza , João Victor Frazão Camara , Flavio Henrique-Silva , Marcos Chiaratti , Angélica Santos , Lindomar Alves , Milene Ferro , Ana Carolina Magalhães , Marília Afonso Rabelo Buzalaf

Objective

In this in vivo proof-of-concept study, acquired pellicle engineering was implemented to promote alterations in the protein composition of the acquired enamel pellicle (AEP) and the bacterial composition of the dental biofilm after treatment with Sugarcane cystatin (CaneCPI-5).

Design

After prophylaxis, 10 volunteers rinsed (10 mL, 1 min) with the following solutions: 1) deionized water (H2O- negative control or 2) 0.1 mg/mL CaneCPI-5. The AEP and biofilm were formed along 2 or 3 h, respectively. The AEP was collected with electrode filter papers soaked in 3 % citric acid. After protein extraction, samples were analyzed by quantitative shotgun label-free proteomics. The biofilm microbiome was collected with a dental curette. The DNA was extracted, amplified, and analyzed by 16S-rRNA Next Generation Sequencing (NGS).

Results

Treatment with CaneCPI-5 increased several proteins with antimicrobial, acid-resistance, affinity for hydroxyapatite, structural and calcium binding properties, such as Cysteine-rich-3 (6-fold-p = 0.03), Cystatin-B (5.5-fold-p < 0.01), Neutrophil-defensin 1 (4.7-fold-p < 0.01), Mucin (3.9-fold-p < 0.01), Immunoglobulin-heavy-constant (3.8-fold-p < 0.01) and Lactotransferrin (2.8-fold-p < 0.01). Microbiome revealed that several commensal bacteria had their abundance increased after rinsing with CaneCPI-5, such as Corynebacterium and Neisseria, while Streptococcus and Prevotella nigrescens were decreased. The results indicate the efficiency of CaneCPI-5 in promoting beneficial changes in the AEP and biofilm, making this phytocystatin a potential target for incorporation into dental products.

Conclusion

Cane demonstrated the capability to alter the protein composition of the acquired enamel pellicle (AEP) and the initial colonizers of the biofilm, enhancing the presence of proteins and bacteria crucial for dental protection.

目的:在这项体内概念验证研究中,采用获得性釉质小泡工程来促进获得性釉质小泡(AEP)蛋白质组成的改变,以及使用甘蔗胱抑素(CaneCPI-5)治疗后牙齿生物膜细菌组成的改变:设计:预防性治疗后,10 名志愿者用以下溶液冲洗(10 毫升,1 分钟):1) 去离子水(H2O-阴性对照)或 2) 0.1 mg/mL CaneCPI-5。2 或 3 小时后分别形成 AEP 和生物膜。用浸泡在 3% 柠檬酸中的电极滤纸收集 AEP。提取蛋白质后,采用无标记蛋白质组学对样本进行定量分析。生物膜微生物组是用牙科刮刀收集的。提取、扩增 DNA,并通过 16S-rRNA 下一代测序(NGS)进行分析:结果:用 CaneCPI-5 处理后,几种具有抗菌、耐酸、与羟基磷灰石亲和、结构和钙结合特性的蛋白质增加了,如富半胱氨酸-3(6 倍-p = 0.03)、胱抑素-B(5.5 倍-p 结论:CaneCPI-5 能改变牙龈微生物群的结构,使其具有抗菌、耐酸、与羟基磷灰石亲和、结构和钙结合特性:甘蔗能够改变获得性釉质细胞膜(AEP)和生物膜初始定植者的蛋白质组成,增加对牙齿保护至关重要的蛋白质和细菌的存在。
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引用次数: 0
TTYH3 promotes the malignant progression of oral squamous cell carcinoma SCC-9 cells by regulating tumor-associated macrophage polarization TTYH3 通过调控肿瘤相关巨噬细胞的极化促进口腔鳞状细胞癌 SCC-9 细胞的恶性发展
IF 2.2 4区 医学 Q1 Medicine Pub Date : 2024-06-15 DOI: 10.1016/j.archoralbio.2024.106028
Yuhui Cao, Zhihui Zhou, Shuai He, Wenhui Liu

Objective

This study was designed to investigate the biological role and the reaction mechanism of Tweety family member 3 (TTYH3) in oral squamous cell carcinoma (OSCC).

Design

The mRNA and protein expressions of TTYH3 were assessed with RT-qPCR and western blot. After silencing TTYH3 expression, the proliferation of OSCC cells were detected using cell counting kit-8 (CCK-8) assay, 5-ethynyl-2′-deoxyuridine (EdU) staining and colony formation assay. Cell migration and invasion were detected using wound healing and transwell. Gelatin zymography protease assay was used to detect matrix metalloproteinase-2 (MMP2) and matrix metalloproteinase-2 (MMP9) activity and western blot was used to detect the expressions of proteins associated with proliferation and epithelial-mesenchymal transition (EMT). The mRNA expression of TTYH3 in THP-1-derived macrophage was detected using real-time reverse transcriptase-polymerase chain reaction (RT-qPCR). The number of CD86-positive cells and CD206-positive cells was detected using immunofluorescence assay. RT-qPCR was used to detect the expressions of M2 markers arginase 1 (ARG1), chitinase-like 3 (YM1) and mannose receptor C-type 1 (MRC1).

Results

In this study, it was found that TTYH3 expression was upregulated in OSCC cell lines and TTYH3 knockdown could inhibit the proliferation, migration, invasion and EMT process in OSCC via suppressing M2 polarization of tumor-associated macrophages.

Conclusions

Collectively, TTYH3 facilitated the progression of OSCC through the regulation of tumor-associated macrophages polarization.

设计采用RT-qPCR和Western blot检测TTYH3的mRNA和蛋白表达。沉默TTYH3表达后,用细胞计数试剂盒-8(CCK-8)测定、5-乙炔基-2′-脱氧尿苷(EdU)染色和集落形成试验检测OSCC细胞的增殖。使用伤口愈合和转孔检测细胞迁移和侵袭。明胶酶谱蛋白酶检测法用于检测基质金属蛋白酶-2(MMP2)和基质金属蛋白酶-2(MMP9)的活性,Western 印迹法用于检测增殖和上皮-间质转化(EMT)相关蛋白的表达。采用实时逆转录聚合酶链反应(RT-qPCR)检测THP-1衍生巨噬细胞中TTYH3的mRNA表达。采用免疫荧光法检测 CD86 阳性细胞和 CD206 阳性细胞的数量。用 RT-qPCR 检测 M2 标志物精氨酸酶 1(ARG1)、几丁质酶样 3(YM1)和甘露糖受体 C 型 1(MRC1)的表达。结果本研究发现,TTYH3在OSCC细胞系中表达上调,敲除TTYH3可抑制肿瘤相关巨噬细胞的M2极化,从而抑制OSCC的增殖、迁移、侵袭和EMT过程。
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引用次数: 0
期刊
Archives of oral biology
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