Archives of oral biology最新文献_第6页

TWF1 promotes tumor progression in head and neck squamous cell carcinoma via AKT phosphorylation TWF1通过AKT磷酸化促进头颈部鳞状细胞癌的肿瘤进展。

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2025-10-14 DOI: 10.1016/j.archoralbio.2025.106425

Yingshun Yang , Kaicheng Yang , Shixiong Peng , Shasha Man , He Chen

Objective

To investigate the molecular mechanisms underlying how Twinfilin actin-binding protein 1 (TWF1) drives the malignant progression of head and neck squamous cell carcinoma (HNSCC).

Design

A pan-cancer analysis of TCGA datasets was performed to evaluate TWF1 expression and prognostic significance, followed by validation using quantitative real-time PCR (qPCR) and immunohistochemistry (IHC) in clinical HNSCC specimens. Functional assays (CCK-8, colony formation, wound healing, and Transwell invasion) were conducted following siRNA-mediated TWF1 knockdown in SCC9 cells. Additionally, the same functional assays were conducted in an HSC3 cell model overexpressing TWF1 to further evaluate its oncogenic function. The involvement of the AKT signaling pathway was examined by Western blot and validated through rescue experiments with the AKT activator SC79.

Results

TWF1 was found to be upregulated in 13 cancer types, including HNSCC. Elevated TWF1 expression, advanced T stage, and lymph node metastasis were identified as independent prognostic indicators in HNSCC. qPCR confirmed increased TWF1 expression, and IHC showed higher TWF1 protein levels in HNSCC tissues. TWF1 knockdown suppressed SCC9 cell proliferation, migration, and invasion, and reduced phosphorylated AKT levels. Conversely, TWF1 overexpression in HSC3 cells promoted proliferation, migration, and invasion while enhancing AKT phosphorylation. SC79 treatment partially reversed the malignant phenotypes suppressed by TWF1 knockdown, supporting TWF1’s regulatory role through AKT phosphorylation.

Conclusion

TWF1 acts as an oncogenic driver in HNSCC, promoting tumor progression through the AKT signaling pathway. It may serve as a potential therapeutic target in precision medicine for HNSCC.

目的：探讨Twinfilin actin-binding protein 1 （TWF1）驱动头颈部鳞状细胞癌（HNSCC）恶性进展的分子机制。设计：对TCGA数据集进行泛癌分析，评估TWF1的表达和预后意义，随后使用定量实时PCR （qPCR）和免疫组织化学（IHC）对临床HNSCC标本进行验证。在SCC9细胞中sirna介导的TWF1敲除后进行功能测定（CCK-8、菌落形成、伤口愈合和Transwell侵袭）。此外，在过表达TWF1的HSC3细胞模型中进行了相同的功能测定，以进一步评估其致癌功能。通过Western blot检测AKT信号通路的参与情况，并通过AKT激活剂SC79的拯救实验进行验证。结果：在包括HNSCC在内的13种癌症类型中发现TWF1表达上调。TWF1表达升高、T分期晚期和淋巴结转移被确定为HNSCC的独立预后指标。qPCR证实TWF1表达升高，IHC显示HNSCC组织中TWF1蛋白水平升高。TWF1敲除抑制SCC9细胞增殖、迁移和侵袭，降低磷酸化AKT水平。相反，在HSC3细胞中，TWF1过表达促进增殖、迁移和侵袭，同时增强AKT磷酸化。SC79治疗部分逆转了TWF1敲低抑制的恶性表型，支持了TWF1通过AKT磷酸化的调节作用。结论：TWF1在HNSCC中发挥致癌驱动作用，通过AKT信号通路促进肿瘤进展。它可能成为HNSCC精准医学的潜在治疗靶点。

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引用次数: 0

Topical effect of polysaccharide cashew gum on the mitigation of dentin erosion progression under in vitro supraesophageal reflux simulation 腰果多糖对体外食管上反流模拟下牙本质侵蚀进展的局部影响

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2025-10-14 DOI: 10.1016/j.archoralbio.2025.106427

Thaysa Fernandes Pinto Mendes , Adrianne Caroline Pereira dos Santos Lima , Eva Aline Costa Cutrim , Paulo Sérgio de Araújo Sousa , Durcilene Alves da Silva , Lucas Antonio Duarte Nicolau , Leily Macedo Firoozmand

Objective

To evaluate, in vitro, the topical effect of polysaccharide cashew gum (PLS-cg) on dentine subjected to erosive cycles that simulate refluxate contact in the oral cavity.

Design

Human dentine blocks were prepared, standardized, and randomly assigned to one of three treatment groups (n = 10): control group (C) without treatment, commercial dental varnish (CDV) 5 % NaF fluoride varnish, and PLS-cg extract (experimental group). The baseline enamel superficial microhardness (HK) and roughness (Ra) were assessed. The dentin was subjected to erosive cycles (6 × 5 min/day for 9 days) using a hydrochloric acid–pepsin solution (pH 2; 0.75-mg/mL pepsin). Surface roughness (Ra), microhardness (HK), percentage surface hardness loss (%SHL), and topographic analysis with scanning electron microscopy (SEM) were used to evaluate the protective heteropolysaccharide effect. Predictive molecular docking simulations were used to investigate possible interactions between PLS-cg and metalloproteinases (MMP2 and MMP9). The data were analyzed with two-way analysis of variance, t test, Pearson’s correlation, and Tukey’s test (p < 0.05).

Results

PLS-cg preserved HK significantly better than the control group (p < 0.001), with the lowest %SHL (-14.04 ± 8.22) and minimal Ra changes (0.124 ± 0.005). Pearson’s correlation between Ra and %SHL was significant and positive (0.7740, p < 0.001). SEM analysis revealed more prominent dentinal tubule openings in the control group, whereas CDV and PLS-cg showed tubule obliteration. Molecular docking identified possible interactions between PLS-cg and MMPs.

Conclusion

The findings indicate that PLS-cg may reduce dentine erosion caused by supraesophageal manifestations of gastroesophageal reflux disease.

目的：体外评价腰果多糖（PLS-cg）对牙本质在模拟口腔内反流接触的侵蚀循环中的局部作用。设计：将人牙本质块制备、标准化并随机分配到三个治疗组（n = 10）中的一个：对照组(C)未治疗，商用牙清漆（CDV） 5 % NaF氟化物清漆和PLS-cg提取物（实验组）。评估基线牙釉质表面显微硬度（HK）和粗糙度（Ra）。使用盐酸-胃蛋白酶溶液（pH 2； 0.75 mg/mL胃蛋白酶）对牙本质进行侵蚀循环（6 × 5 分钟/天，持续9天）。采用表面粗糙度（Ra）、显微硬度（HK）、表面硬度损失率（%SHL）和扫描电镜形貌分析（SEM）来评价杂多糖的保护作用。预测分子对接模拟用于研究PLS-cg与金属蛋白酶（MMP2和MMP9）之间可能的相互作用。对数据进行双向方差分析、t检验、Pearson相关和Tukey检验（p ）结果：PLS-cg保存HK显著优于对照组（p ）结论：PLS-cg可减少胃食管反流病食管上表现引起的牙本质糜烂。

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引用次数: 0

Combined bioinformatics and experimental validation identifies m1A-modified genes as potential diagnostic biomarkers in periodontitis and diabetes 结合生物信息学和实验验证确定m1a修饰基因作为牙周炎和糖尿病的潜在诊断生物标志物。

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2025-10-14 DOI: 10.1016/j.archoralbio.2025.106428

Kai Wang , Aiguo Wang , Liewang Qiu , Ling Xu , Jingjing Wei

Objective

To identify m1A-modified genes with diagnostic potential linking periodontitis and type 2 diabetes mellitus (T2DM) by integrating bioinformatics and experimental validation.

Design

Transcriptomic data for periodontitis and T2DM patients were integrated from the GEO database to analyze m1A-related gene expression. A diagnostic model was constructed using ridge and logistic regression. Gene function enrichment, immune infiltration, and protein-protein interaction analyses explored m1A regulatory mechanisms based on m1A scoring and patient clustering models. Gingival tissue samples were collected from periodontitis patients and healthy controls, and a streptozotocin-induced diabetic β-cell model was established. qRT-PCR was performed to validate candidate genes (RRP8, ALKBH3, MAK16, and DDX18). Statistical comparisons were conducted using the non-parametric Mann–Whitney U test.

Results

Several m1A-related genes were differentially expressed in both periodontitis and T2DM. RRP8 and ALKBH3 had high predictive value, with area under the curve (AUC) values of 0.80 (periodontitis) and 0.72 (T2DM). m1A scoring and patient clustering models effectively distinguished patient groups with distinct transcriptomic and immune profiles. Hub genes MAK16 and DDX18 showed consistent expression patterns and strong correlations with immune infiltration. qRT-PCR confirmed significant downregulation of RRP8, ALKBH3, MAK16, and DDX18 in inflamed gingival tissues, and upregulation in the diabetic cell model (p < 0.05), supporting the bioinformatics findings.

Conclusions

This integrative study identifies key m1A-modified genes potentially linking periodontitis and diabetes. The combination of bioinformatics analysis and experimental validation highlights their potential as diagnostic biomarkers and provides novel insights into shared molecular mechanisms of these comorbid conditions.

目的：通过生物信息学和实验验证相结合的方法，鉴定具有牙周炎与2型糖尿病（T2DM）诊断潜力的m1a修饰基因。设计：从GEO数据库中整合牙周炎和T2DM患者的转录组学数据，分析m1a相关基因表达。采用岭回归和逻辑回归建立诊断模型。基于m1A评分和患者聚类模型，基因功能富集、免疫浸润和蛋白-蛋白相互作用分析探索了m1A的调控机制。收集牙周炎患者和健康对照者的牙龈组织标本，建立链脲佐菌素诱导的糖尿病β细胞模型。采用qRT-PCR验证候选基因（RRP8、ALKBH3、MAK16和DDX18）。采用非参数Mann-Whitney U检验进行统计比较。结果：几个m1a相关基因在牙周炎和T2DM中均有差异表达。RRP8和ALKBH3具有较高的预测价值，曲线下面积（AUC）值分别为0.80（牙周炎）和0.72 （T2DM）。m1A评分和患者聚类模型有效区分具有不同转录组和免疫谱的患者组。枢纽基因MAK16和DDX18表达模式一致，且与免疫浸润有很强的相关性。qRT-PCR证实了炎症牙龈组织中RRP8、ALKBH3、MAK16和DDX18的显著下调，以及糖尿病细胞模型中的上调（p ）。结论：这项综合研究鉴定了关键的ma1修饰基因，可能与牙周炎和糖尿病有关。生物信息学分析和实验验证的结合突出了它们作为诊断性生物标志物的潜力，并为这些共病的共同分子机制提供了新的见解。

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引用次数: 0

Fucose as a biomarker in oral potentially malignant disorders and oral squamous cell carcinoma: A systematic review and meta-analysis focus作为口腔潜在恶性疾病和口腔鳞状细胞癌的生物标志物：系统综述和荟萃分析。

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2025-10-14 DOI: 10.1016/j.archoralbio.2025.106423

Shruti Gupta , Deepti Sharma , Anita Hooda , Mala Kamboj

Objectives

This systematic review and meta-analysis aimed to investigate the potential of fucose as a biomarker for oral potentially malignant disorders (OPMDs) and oral squamous cell carcinoma (OSCC).

Design

Relevant articles were searched across multiple databases, including PubMed, Scopus, Google Scholar, Science Direct, and Clinical Key. Twenty-three studies that met the eligibility criteria were included for qualitative review; out of which, 20 studies that provided data for comparison between study and control groups were further subjected to meta-analysis.

Results

The analysis revealed significantly high fucose levels in the saliva of OPMD patients (p = 0.013) and OSCC patients (p = 0.003) compared to healthy controls. Additionally, fucose levels were elevated in the serum of patients with OSCC (p < 0.001) and oral leukoplakia (p = 0.027) compared to controls.

Conclusion

The findings of this meta-analysis suggest that fucose could serve as a potential biomarker for OPMDs and OSCC. However, the presence of significant heterogeneity in serum evaluation and the limited studies focusing on saliva indicate the need for further investigation.

目的：本系统综述和荟萃分析旨在探讨病灶作为口腔潜在恶性疾病（OPMDs）和口腔鳞状细胞癌（OSCC）生物标志物的潜力。设计：在多个数据库中检索相关文章，包括PubMed、Scopus、谷歌Scholar、Science Direct和Clinical Key。23项符合资格标准的研究被纳入定性评价；其中，有20项研究提供了实验组与对照组比较的数据，进一步进行meta分析。结果：与健康对照组相比，OPMD患者（p = 0.013）和OSCC患者（p = 0.003）唾液中的病灶水平显著升高。此外，OSCC患者血清中的病灶水平升高（p ）。结论：本荟萃分析结果表明，病灶可作为opmd和OSCC的潜在生物标志物。然而，在血清评估中存在显著的异质性和有限的研究集中在唾液表明需要进一步的研究。

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引用次数: 0

Optimization and evaluation of antibacterial extracts from Acacia nilotica, Psidium guajava, Syzygium aromaticum, and Salvadora persica to inhibit dental caries 金合欢、番石榴、香薷、桃金娘抑菌提取物抑龋效果的优化与评价。

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2025-10-14 DOI: 10.1016/j.archoralbio.2025.106426

Chamundeswari Kandasamy , Arunadevi Baladhandapani , Renuka Devi Ponnuswamy , Hemananthan Eswaran , Vinodhini Karthikeyan , Saranya Rajendran

Objective

Dental caries is a significant global issue, which affects approximately 44 % of the global population. The objective of this research is to extract crude phytochemicals from Psidium guajava, Acacia nilotica, Syzygium aromaticum, and Salvadora persica and also optimizing using RSM. Therefore, this aims to maximize the antibacterial potential of the extracts at effective inhibition of dental caries

Method

The crude extracts from the plants samples, with varying extraction parameters, such as ethanol concentrations, temperatures, and solid-solvent ratios was done using Soxhlet method and antibacterial activity was determined through well and disc diffusion methods along with optimization was performed by Response Surface Methodology (RSM).

Results

The results depict that Psidium guajava exhibited the strongest antibacterial activity against S. mutans and Lactobacillus, with inhibition zones of 36 mm & 20 mm and 32 mm & 24 mm for both well and disc diffusion. Similarly, Syzygium aromaticum also shows slight stronger inhibition zones, Acacia nilotica exhibited moderate activity and in contrast, Salvadora persica showed limited activity. RSM optimization was validated and with well fitted quadratic models R² values ranging from 0.69 to 0.79. Predicted and experimental values are in close agreement between with differences below 0.5 %.

Conclusion

These findings suggest that P. guajava, S. aromaticum and A. nilotica have strong potential as natural antibacterial agents in oral care formulations.

目的：龋齿是一个重要的全球性问题，影响了全球约44% %的人口。本研究的目的是提取番石榴、金合欢、香合木和木耳的粗化学成分，并对其进行RSM优化。方法：采用索氏法提取不同提取参数（乙醇浓度、温度、固液比）的植物粗提物，采用孔法测定其抑菌活性，并采用响应面法（RSM）进行优化。结果：瓜石榴对变形链球菌和乳酸菌的抑菌活性最强，孔扩散区和盘扩散区分别为36 mm和20 mm和32 mm和24 mm。同样，Syzygium aromaticum也表现出稍强的抑制区，Acacia nilotica表现出中等的活性，而Salvadora persica则表现出有限的活性。二次模型拟合良好，R²值在0.69 ~ 0.79之间。预测值与实验值非常吻合，误差在0.5 %以下。结论：番石榴、香薷和尼罗花在口腔护理制剂中具有很强的天然抗菌潜力。

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引用次数: 0

Tooth loss induces cognitive decline independent of low-protein diet intake in male mice 在雄性小鼠中，牙齿脱落导致认知能力下降，与低蛋白饮食摄入无关。

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2025-10-11 DOI: 10.1016/j.archoralbio.2025.106421

Rie Hatakeyama , Hiroshi Oue , Miyuki Yokoi , Eri Ishida , Takayasu Kubo , Kazuhiro Tsuga

Objectives

Previous articles have shown that tooth loss is associated with cognitive decline in animal and human studies. Additionally, poor nutritional status including low-protein diet is associated with dementia. The evidence of the association of tooth loss and nutritional status is uncertain. The purpose of this study was to investigate the relationship between low-protein diet intake and cognitive decline following tooth loss in mice.

Design

Male senescence-accelerated mouse-prone 8 mice were randomly allocated into sham-operated control and tooth loss groups with extracted maxillary molars: control with normal-protein diet; control with low-protein diet; bilateral maxillary molar extraction with normal-protein diet; and bilateral maxillary molar extraction with low-protein diet. After 6 months, a behavioral test was conducted, and mRNA expression and immunohistochemistry in the brain were analyzed.

Results

Behavioral test revealed no effect of the interaction between tooth loss and low-protein diet intake on cognitive decline; however, tooth loss had a marked effect on cognitive decline. Real-time polymerase chain reaction showed no interaction between tooth loss and low-protein intake for Bax/Bcl-2 mRNA expression; however, tooth loss had a significant effect on Bax/Bcl-2 mRNA expression. Immunohistochemical analysis showed that the effect of tooth loss in neuronal inflammation and neuronal loss were observed in CA1 and DG region, but the effect of low-protein diet was limited in CA3.

Conclusion

The present study revealed that the impact of tooth loss on cognitive decline was not dependent on the low-protein diet condition.

目的：以前的文章已经在动物和人类研究中表明，牙齿脱落与认知能力下降有关。此外，营养状况不佳，包括低蛋白饮食与痴呆症有关。牙齿脱落与营养状况之间关系的证据尚不确定。本研究的目的是探讨低蛋白饮食摄入与小鼠牙齿脱落后认知能力下降之间的关系。设计：雄性衰老加速小鼠8只，随机分为假手术对照组和拔除上颌磨牙的牙齿脱落组；对照组采用低蛋白饮食；正常蛋白饮食下双侧上颌磨牙拔牙；双侧上颌磨牙拔牙采用低蛋白饮食。6个月后进行行为学测试，分析脑内mRNA表达和免疫组化。结果：行为学测试未发现缺牙与低蛋白饮食的交互作用对认知能力下降有影响；然而，牙齿脱落对认知能力下降有显著影响。实时聚合酶链反应显示，牙齿脱落与低蛋白摄入对Bax/Bcl-2 mRNA表达无交互作用；而缺牙对Bax/Bcl-2 mRNA表达有显著影响。免疫组化分析显示，在CA1区和DG区观察到牙齿脱落对神经元炎症和神经元丢失的影响，但低蛋白饮食对CA3区的影响有限。结论：本研究揭示了牙齿脱落对认知能力下降的影响不依赖于低蛋白饮食条件。

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引用次数: 0

B. lactis HN019 modulates periapical inflammation and enhances cementum repair by increasing CEMP-1 expression in an animal model 在动物模型中，B. lactis HN019通过增加cmp -1的表达来调节根尖周围炎症并促进骨质修复。

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2025-10-10 DOI: 10.1016/j.archoralbio.2025.106420

Lisa Danielly Curcino Araujo , Ana Paula Gomes-Moura , Raquel Assed Bezerra da Silva , Michel Reis Messora , Flávia Aparecida Chaves Furlaneto , Sérgio Luis de Souza Salvador , Allan Radaic , Pachiyappan Kamarajan , Yvonne L. Kapila , Paulo Nelson-Fillho , Higinio Arzate , Samin Sirous , Geeta Bhuvanagiri , Léa Assed Bezerra da Silva

Objective

To investigate the effect of the HN019 probiotic on the modulation of inflammation and the resorption of mineralized tissues in experimentally induced periapical lesions in an animal model, evaluating its impact on the expression of cementum protein 1 (CEMP-1).

Design

Periapical lesion was induced in 45 Wistar rats. The animals were divided into groups according to the irrigating solution used. Root canals were irrigated on days 7 and 14. After 21 days, the animals were euthanized, and mandibles were processed for histological analysis. Descriptive and semi-quantitative evaluations of the inflammatory infiltrate, periodontal ligament, bone and cementum resorption, as well as counts of inflammatory cells were performed using HE staining. Also, to assess cementum formation, tissue sections were stained with an antibody for CEMP-1 and analyzed by immunohistochemistry.

Results

In the Infection + Probiotic Group, the inflammatory infiltrate was mixed and diffuse, ranging from mild to severe, with no statistically significant difference compared to the Control Group (p > 0.05). However, in the Infection + Probiotic Group, cementum resorption was significantly lower compared to Control Group (p < 0.001). Moreover, CEMP-1 expression was significantly higher in Infection + Probiotic-Group compared with all other groups (p < 0.0001).

Conclusion

The probiotic HN019 significantly increased CEMP-1 expression, indicating cementum formation in periapical lesions in vivo. These findings suggest that B. lactis HN019 may have regenerative potential in clinical scenarios. Furthermore, this strain appears to modulate the inflammatory state of periapical lesions toward a reparative phenotype, characterized by reduced bone resorption. Also, inflammatory infiltrate was like control group.

目的：通过动物模型研究HN019益生菌对实验性根尖周病变中炎症和矿化组织吸收的调节作用，评估其对骨水泥蛋白1 （cementum protein 1, cmp -1）表达的影响。设计：45只Wistar大鼠根尖周围病变。根据所使用的灌洗液进行分组。在第7天和第14天进行根管冲洗。21天后，对动物实施安乐死，并对下颌骨进行组织学分析。采用HE染色对炎症浸润、牙周韧带、骨骨质吸收、炎症细胞计数进行描述性和半定量评价。此外，为了评估骨质形成，组织切片用cmp -1抗体染色，并通过免疫组织化学分析。结果：感染+ 益生菌组炎症浸润呈混合性、弥漫性，轻重有别，与对照组比较差异无统计学意义（p > 0.05）。而感染+ 益生菌组骨质吸收明显低于对照组（p ）结论：益生菌HN019显著提高了cmp -1的表达，提示体内根尖周病变中骨质形成。这些发现提示乳杆菌HN019在临床可能具有再生潜能。此外，这种菌株似乎可以调节根尖周围病变的炎症状态，使其向修复表型发展，其特征是骨吸收减少。炎症浸润与对照组相似。

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引用次数: 0

Mirt2 alleviates LPS-induced inflammation of osteoblasts in alveolar bone destruction Mirt2可减轻lps诱导的成骨细胞在牙槽骨破坏中的炎症反应

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2025-10-09 DOI: 10.1016/j.archoralbio.2025.106410

Yajie Wu , Zhifei Su , Bowen Zhang , Lei Cheng , Ziyou Wang , He Yuan , Jiyao Li

Objectives

The long noncoding RNA (lncRNA) myocardial infarction–associated transcript 2 (Mirt2) has been confirmed to affect several inflammatory diseases. This study was conducted to explore the functional mechanism of Mirt2 in inflammatory alveolar bone loss and its possibility of being a therapeutic target.

Design

The expression level and potential role of Mirt2 in chronic inflammatory alveolar bone loss in mouse models of periodontitis and periapical periodontitis were investigated using micro-CT and qPCR. The characteristics of Mirt2 were evaluated by FISH, qPCR, ELISA, and alkaline phosphatase staining to confirm its function and mechanism of action in inflammatory response.

Results

Mirt2 expression was significantly enriched in inflammatory alveolar bone diseases. Mirt2 expression increased upon LPS stimulation in MC3T3-E1 cells (P < 0.05), located at the cell cytoplasm. Mirt2 knockdown exacerbated the LPS-stimulated inflammatory response in MC3T3-E1 cells, whereas Mirt2 overexpression attenuated this effect and rescued LPS-impaired osteogenic differentiation.

Conclusions

lncRNA Mirt2 suggests a potential role in chronic inflammation–related bone loss, providing potential therapeutic target worthy of future investigation for inflammation-related bone loss.

目的长链非编码RNA （lncRNA）心肌梗死相关转录物2 （Mirt2）已被证实影响多种炎症性疾病。本研究旨在探讨Mirt2在炎症性牙槽骨丢失中的作用机制及其作为治疗靶点的可能性。设计采用micro-CT和qPCR方法研究Mirt2在牙周炎和根尖周炎小鼠慢性炎症性牙槽骨丢失中的表达水平及其潜在作用。通过FISH、qPCR、ELISA、碱性磷酸酶染色等方法评价Mirt2的特征，确认其在炎症反应中的功能和作用机制。结果smirt2在炎症性牙槽骨疾病中表达显著富集。MC3T3-E1细胞在LPS刺激下Mirt2表达增加（P <； 0.05），位于细胞质中。Mirt2敲低加重了lps刺激的MC3T3-E1细胞的炎症反应，而Mirt2过表达减弱了这种作用，并挽救了lps受损的成骨分化。结论slncrna Mirt2可能在慢性炎症相关性骨质流失中发挥潜在作用，为炎症相关性骨质流失提供了值得未来研究的潜在治疗靶点。

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引用次数: 0

Hsa_circ_0007349 upregulates glutamate-ammonia ligase to modulate gingival fibroblast wound healing Hsa_circ_0007349上调谷氨酸氨连接酶调节牙龈成纤维细胞创面愈合

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2025-10-08 DOI: 10.1016/j.archoralbio.2025.106419

Liang Ren , Linlin He , Runyu Zhang , Xiaoyi Li , Shimeng Xiao , Shujuan Guo , Chengcheng Liu , Yi Ding

Objectives

Human gingival fibroblasts (HGFs) are important cells that maintain the structure and integrity of periodontal tissues. Circular RNAs (circRNAs) play critical roles in cellular processes of periodontitis; however, their roles in HGFs remain unclear. This study aimed to elucidate the function and mechanism of a novel circRNA, hsa_circ_0007349, in periodontitis progression by validating its existence, establishing its role in a ceRNA network (targeting miR-127–5p/GLUL), assessing its regulatory effects on HGF functions in vitro, and confirming its pathological impact in a murine periodontitis model.

Design

We performed circRNA sequencing to identify differentially expressed circRNAs between periodontitis and healthy gingival tissues. We selected hsa_circ_0007349, and confirmed its circular structure by Sanger sequencing, RNase R and actinomycin D assays. hsa_circ_0007349/miR-127–5p/glutamate-ammonia ligase (GLUL) interactions were explored using bioinformatics analysis, dual-luciferase reporter assays, RT-qPCR, and western blotting, and their effects on HGF functions were assessed via transcriptome sequencing, Cell Counting Kit-8, cell scratch, and Transwell assays. To further investigate the pathogenic mechanisms, we established a standardized murine periodontitis model using female C57BL/6 mice, followed by histopathological evaluation of periodontal tissue destruction.

Results

Hsa_circ_0007349 expression significantly increased in gingival tissues of patients with periodontitis and in HGFs treated with Porphyromonas gingivalis lipopolysaccharide. hsa_circ_0007349 regulates miR-127–5p to affect cell proliferation, migration, and wound healing, and affects extracellular matrix metabolism and periodontal tissue damage via GLUL.

Conclusion

Hsa_circ_0007349 acts as a competing endogenous RNA to promote HGF proliferation, migration, wound healing and extracellular matrix turnover by targeting miR-127–5p and GLUL.

目的：人牙龈成纤维细胞是维持牙周组织结构和完整性的重要细胞。环状rna （circRNAs）在牙周炎的细胞过程中发挥关键作用；然而，它们在hgf中的作用尚不清楚。本研究旨在通过验证一种新的circRNA hsa_circ_0007349在牙周炎进展中的功能和机制，建立其在ceRNA网络中的作用（靶向miR-127-5p /GLUL），评估其体外对HGF功能的调节作用，并确认其在小鼠牙周炎模型中的病理影响。我们进行了环状rna测序，以鉴定牙周炎和健康牙龈组织之间表达差异的环状rna。我们选择了hsa_circ_0007349，并通过Sanger测序、RNase R和放线菌素D检测证实了其环状结构。hsa_circ_0007349/ miR-127-5p /谷氨酸氨连接酶（GLUL）相互作用采用生物信息学分析、双荧光素酶报告基因检测、RT-qPCR和western blotting检测，并通过转录组测序、细胞计数试剂盒-8、细胞划痕和Transwell检测评估其对HGF功能的影响。为了进一步探讨其致病机制，我们以雌性C57BL/6小鼠为实验对象，建立了标准的小鼠牙周炎模型，并对其牙周组织破坏情况进行了组织病理学评价。结果shsa_circ_0007349在牙周炎患者的牙龈组织和牙龈卟啉单胞菌脂多糖治疗的hgf中表达显著升高。hsa_circ_0007349调节miR-127-5p影响细胞增殖、迁移和创面愈合，并通过GLUL影响细胞外基质代谢和牙周组织损伤。结论hsa_circ_0007349作为一种竞争性内源性RNA，通过靶向miR-127-5p和GLUL促进HGF增殖、迁移、伤口愈合和细胞外基质更新。

{"title":"Hsa_circ_0007349 upregulates glutamate-ammonia ligase to modulate gingival fibroblast wound healing","authors":"Liang Ren , Linlin He , Runyu Zhang , Xiaoyi Li , Shimeng Xiao , Shujuan Guo , Chengcheng Liu , Yi Ding","doi":"10.1016/j.archoralbio.2025.106419","DOIUrl":"10.1016/j.archoralbio.2025.106419","url":null,"abstract":"<div><h3>Objectives</h3><div>Human gingival fibroblasts (HGFs) are important cells that maintain the structure and integrity of periodontal tissues. Circular RNAs (circRNAs) play critical roles in cellular processes of periodontitis; however, their roles in HGFs remain unclear. This study aimed to elucidate the function and mechanism of a novel circRNA, hsa_circ_0007349, in periodontitis progression by validating its existence, establishing its role in a ceRNA network (targeting miR-127–5p/GLUL), assessing its regulatory effects on HGF functions in vitro, and confirming its pathological impact in a murine periodontitis model.</div></div><div><h3>Design</h3><div>We performed circRNA sequencing to identify differentially expressed circRNAs between periodontitis and healthy gingival tissues. We selected hsa_circ_0007349, and confirmed its circular structure by Sanger sequencing, RNase R and actinomycin D assays. hsa_circ_0007349/miR-127–5p/glutamate-ammonia ligase (GLUL) interactions were explored using bioinformatics analysis, dual-luciferase reporter assays, RT-qPCR, and western blotting, and their effects on HGF functions were assessed via transcriptome sequencing, Cell Counting Kit-8, cell scratch, and Transwell assays. To further investigate the pathogenic mechanisms, we established a standardized murine periodontitis model using female C57BL/6 mice, followed by histopathological evaluation of periodontal tissue destruction.</div></div><div><h3>Results</h3><div>Hsa_circ_0007349 expression significantly increased in gingival tissues of patients with periodontitis and in HGFs treated with <em>Porphyromonas gingivalis</em> lipopolysaccharide. hsa_circ_0007349 regulates miR-127–5p to affect cell proliferation, migration, and wound healing, and affects extracellular matrix metabolism and periodontal tissue damage via GLUL.</div></div><div><h3>Conclusion</h3><div>Hsa_circ_0007349 acts as a competing endogenous RNA to promote HGF proliferation, migration, wound healing and extracellular matrix turnover by targeting miR-127–5p and GLUL.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"180 ","pages":"Article 106419"},"PeriodicalIF":2.1,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145263629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Distinct oral microbiome profiles in substance users in the high-incidence Mizo tribal community, Northeast India 印度东北部高发病率米佐部落社区药物使用者的口腔微生物群特征

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2025-10-08 DOI: 10.1016/j.archoralbio.2025.106418

Lalfak Zuali , Lalchhanhima Ralte , John Zothanzama , Christine Vanlalbiakdiki Sailo , Nachimuthu Senthil Kumar

Objectives

To characterize the oral microbiome of substance users (primarily tobacco, alcohol, and opioids) compared to healthy controls in the high-incidence Mizo tribal community of Northeast India, and to examine their association with food and lifestyle habits.

Methods

Saliva samples from 53 substance users and 35 healthy controls were subjected to 16S rRNA (V3–V4 region) sequencing. Alpha and beta diversity analyses, differential abundance testing, and microbial functional prediction were conducted. Statistical analyses were corrected for multiple comparisons using false discovery rate (FDR) adjustment where applicable. Associations with food and lifestyle habits were also examined.

Results

Substance users exhibited significantly lower alpha diversity, and beta diversity analyses revealed distinct clustering between the two groups. Firmicutes_D, Actinobacteria, Rothia, and Streptococcus were more abundant in substance users, whereas Bacteroidota, Proteobacteria, Neisseria, and Prevotella were enriched in healthy controls. Functional predictions indicated upregulation of biofilm formation and xenobiotic degradation pathways in substance users. Exposure to Jhum cultivation, prenatal smoking, and consumption of fermented pork fat (saum) were correlated with microbial composition.

Conclusions

Substance use and associated environmental exposures were linked to oral microbiome dysbiosis. Certain bacterial taxa may serve as potential microbial biomarkers of substance use in this high-risk tribal population, warranting further investigation.

目的：在印度东北部高发病率的米佐部落社区，与健康对照者相比，描述物质使用者（主要是烟草、酒精和阿片类药物）的口腔微生物群特征，并研究它们与食物和生活习惯的关系。方法：对53名药物使用者和35名健康对照者的唾液进行16S rRNA （V3-V4区）测序。进行了α和β多样性分析、差异丰度检验和微生物功能预测。在适用的情况下，使用错误发现率（FDR）调整对多重比较进行统计分析。与食物和生活习惯的关系也被调查。结果：物质使用者表现出明显较低的α多样性，β多样性分析显示两组之间存在明显的聚类。厚壁菌属、放线菌属、罗氏菌属和链球菌在药物使用者中含量较高，而拟杆菌属、变形菌属、奈瑟菌属和普雷沃菌属在健康对照中含量较高。功能预测表明，物质使用者的生物膜形成和外源性降解途径上调。暴露于Jhum培养、产前吸烟和食用发酵猪油（saum）与微生物组成相关。结论：药物使用和相关的环境暴露与口腔微生物群失调有关。某些细菌分类群可能作为该高危部落人群物质使用的潜在微生物生物标志物，值得进一步研究。

{"title":"Distinct oral microbiome profiles in substance users in the high-incidence Mizo tribal community, Northeast India","authors":"Lalfak Zuali , Lalchhanhima Ralte , John Zothanzama , Christine Vanlalbiakdiki Sailo , Nachimuthu Senthil Kumar","doi":"10.1016/j.archoralbio.2025.106418","DOIUrl":"10.1016/j.archoralbio.2025.106418","url":null,"abstract":"<div><h3>Objectives</h3><div>To characterize the oral microbiome of substance users (primarily tobacco, alcohol, and opioids) compared to healthy controls in the high-incidence Mizo tribal community of Northeast India, and to examine their association with food and lifestyle habits.</div></div><div><h3>Methods</h3><div>Saliva samples from 53 substance users and 35 healthy controls were subjected to 16S rRNA (V3–V4 region) sequencing. Alpha and beta diversity analyses, differential abundance testing, and microbial functional prediction were conducted. Statistical analyses were corrected for multiple comparisons using false discovery rate (FDR) adjustment where applicable. Associations with food and lifestyle habits were also examined.</div></div><div><h3>Results</h3><div>Substance users exhibited significantly lower alpha diversity, and beta diversity analyses revealed distinct clustering between the two groups. Firmicutes_D, Actinobacteria, <em>Rothia</em>, and <em>Streptococcus</em> were more abundant in substance users, whereas Bacteroidota, Proteobacteria, <em>Neisseria</em>, and <em>Prevotella</em> were enriched in healthy controls. Functional predictions indicated upregulation of biofilm formation and xenobiotic degradation pathways in substance users. Exposure to Jhum cultivation, prenatal smoking, and consumption of fermented pork fat (saum) were correlated with microbial composition.</div></div><div><h3>Conclusions</h3><div>Substance use and associated environmental exposures were linked to oral microbiome dysbiosis. Certain bacterial taxa may serve as potential microbial biomarkers of substance use in this high-risk tribal population, warranting further investigation.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"180 ","pages":"Article 106418"},"PeriodicalIF":2.1,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145304953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0