Archives of oral biology最新文献_第7页

Exercise modulates local inflammation and tissue loss in experimental periodontitis: A histomorphometric and immunohistochemical study in ra 运动调节实验性牙周炎的局部炎症和组织损失：ra的组织形态学和免疫组织化学研究

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2025-10-18 DOI: 10.1016/j.archoralbio.2025.106435

Gümrükçüoğlu Zehra , Burcu Özkan Çetinkaya , Ferda Pamuk Özer , Sinem İnal , Sevda Kurt Bayrakdar , Muhammed Taha Kaya

Objective

This study aimed to investigate the effects of resistance exercise on the progression of periodontal disease in rats, focusing on alveolar bone loss, attachment loss, and local inflammatory and bone metabolic markers.

Materials and methods

Forty male Wistar albino rats were allocated into four experimental groups in a 2 × 2 factorial design: G1 or G2 and G3 or G4. The exercise protocol consisted of progressive swimming sessions over eight weeks, with resistance provided by weights tied to the animals. In the final week, MBT was performed, and rats were sacrificed for histomorphometric and immunohistochemical analysis.

Results

The values for alveolar bone loss and attachment loss were significantly lower in the exercised groups compared to both the G1 and G2 groups (p < 0.001). Among the rats with periodontitis, TNF-α expression was significantly lower in the exercised group (p = 0.002), whereas IL-10 levels showed no significant differences (p > 0.005). RANKL expression was elevated in G2 (p = 0.039), with no significant variation in OPG among groups. In MBT, G2 showed significantly higher values compared to G1 and G3 (p = 0.004). G4 displayed intermediate values, higher than G3 but lower than G2, without statistical significance (p > 0.005).

Conclusion

Physical exercise reduced TNF-α levels in the presence of periodontitis and increased IL-10, although the latter change was not statistically significant. Exercise also contributed to a decrease in RANKL/OPG expression, suggesting a protective effect on alveolar bone resorption. MBT findings further indicate that exercise may alleviate periodontitis-associated behavioral alterations, supporting its potential role as an adjunctive therapy in managing periodontitis.

目的探讨阻力运动对大鼠牙周病进展的影响，重点关注牙槽骨丢失、附着丧失以及局部炎症和骨代谢标志物。材料与方法40只雄性Wistar白化大鼠按2 × 2因子设计分为4个实验组：G1或G2和G3或G4。锻炼方案包括在八周内进行渐进式游泳，并通过拴在动物身上的重物提供阻力。最后一周进行MBT，处死大鼠进行组织形态学和免疫组化分析。结果运动组的牙槽骨丢失和附着体丢失值明显低于G1和G2组（p <； 0.001）。在牙周炎大鼠中，运动组TNF-α表达明显降低（p = 0.002），而IL-10水平无显著差异（p >； 0.005）。RANKL在G2组表达升高（p = 0.039），OPG组间差异无统计学意义。在MBT中，G2的数值明显高于G1和G3 （p = 0.004）。G4为中间值，高于G3，低于G2，差异无统计学意义（p >； 0.005）。结论体育锻炼可降低牙周炎患者TNF-α水平，升高IL-10水平，但后者的变化无统计学意义。运动还导致RANKL/OPG表达降低，提示运动对牙槽骨吸收有保护作用。MBT研究结果进一步表明，运动可以减轻牙周炎相关的行为改变，支持其作为治疗牙周炎的辅助治疗的潜在作用。

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引用次数: 0

DPM1 expression as a potential prognostic tumor marker in oral squamous cell carcinoma DPM1表达作为口腔鳞状细胞癌的潜在预后肿瘤标志物。

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2025-10-17 DOI: 10.1016/j.archoralbio.2025.106432

Hongyu Liu , Buling Wu

Objective

This study investigates how dolichol phosphate mannose synthase (DPMS) subunits DPM1/2/3 affect oral squamous cell carcinoma (OSCC) prognosis and their associations with OSCC.

Design

To evaluate the connections between DPMS subunits DPM1/2/3 and OSCC, we utilized a wide array of databases and analytical resources, including The Cancer Genome Atlas (TCGA), Metascape, and MethSurv. For initial verification, we applied real-time quantitative polymerase chain reaction (RT-qPCR) to OSCC cell lines and tissues.

Results

DPM1 and DPM2 expression was higher in OSCC tissues than normal ones. Univariate and multivariate COX analyses showed high DPM1 expression independently correlated with poorer OSCC overall survival. Gene ontology (GO) analysis via Metascape revealed DPM1/2/3 and co-expressed genes enriched in cell cycle, nucleic acid metabolism, and translation. Gene set enrichment analysis (GSEA) linked them to pathways like complement activation, BCR signaling, and immune systems. Besides, high DPMS expression was tied to a more unfavorable prognosis in OSCC patients, and DPM1/2/3 was associated with the infiltration of tumor immune cells in OSCC patients. We found that methylation levels might be related to the prognosis of OSCC patients. By means of RT-qPCR, we ascertained the differential expression levels of DPM1 in human normal oral keratinocytes (HOK) and human tongue squamous cell carcinoma cells (Cal27, SCC9), cancer and paracancerous tissues of OSCC.

Conclusion

Our findings imply that DPM1, a subunit of DPMS, can serve as a potential molecular indicator for poor prognosis in OSCC and may act as a novel target in OSCC treatment strategies.

目的：探讨磷酸膦醇甘露糖合成酶（DPMS）亚基DPM1/2/3对口腔鳞状细胞癌（OSCC）预后的影响及其与OSCC的关系。设计：为了评估DPMS亚基DPM1/2/3和OSCC之间的联系，我们利用了广泛的数据库和分析资源，包括the Cancer Genome Atlas （TCGA）、metscape和MethSurv。为了初步验证，我们对OSCC细胞系和组织进行了实时定量聚合酶链反应（RT-qPCR）。结果：DPM1、DPM2在OSCC组织中的表达高于正常组织。单因素和多因素COX分析显示，高DPM1表达与较差的OSCC总生存率独立相关。通过Metascape进行基因本体（GO）分析，发现DPM1/2/3和共表达基因富集于细胞周期、核酸代谢和翻译中。基因集富集分析（GSEA）将它们与补体激活、BCR信号和免疫系统等途径联系起来。此外，DPMS高表达与OSCC患者预后不良相关，DPM1/2/3与OSCC患者肿瘤免疫细胞浸润相关。我们发现甲基化水平可能与OSCC患者的预后有关。通过RT-qPCR，我们确定了DPM1在人正常口腔角质形成细胞（HOK）和人舌鳞癌细胞（Cal27、SCC9）、OSCC癌及癌旁组织中的差异表达水平。结论：DPMS亚基DPM1可作为OSCC不良预后的潜在分子指标，并可能作为OSCC治疗策略的新靶点。

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引用次数: 0

Patterns of circulating autoantibodies against desmoglein-1 and desmoglein-3 in the progression of oral lichen planus: A scoping review 口腔扁平苔藓发展过程中抗粘连蛋白-1和粘连蛋白-3循环自身抗体的模式：范围综述。

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2025-10-17 DOI: 10.1016/j.archoralbio.2025.106431

Andari Sarasati , Kadek Gede Putra Wibawa , Meircurius Dwi Condro Surboyo , Rene Luis Panico , Arvind Babu Rajendra Santosh , Adhistya Viany , Dewi Agustina , Diah Savitri Ernawati

Objective

The potential role of autoantibodies targeting desmosomal proteins, particularly desmoglein 1 (Dsg-1) and desmoglein 3 (Dsg-3), in modulating disease severity and epithelial integrity in oral lichen planus (OLP) remains a subject of growing interest. Distinct patterns of anti-Dsg-1 and anti-Dsg-3 autoantibodies across OLP subtypes may correlate with disease progression. This scoping review investigates the expression patterns of circulating anti-Dsg-1 and anti-Dsg-3 autoantibodies in reticular, erosive, and ulcerative forms of OLP to elucidate their potential roles in epithelial disruption and disease advancement.

Design

Following PRISMA guidelines, a systematic search of four electronic databases was conducted. Eligible studies included case reports, case series, case-control and cross-sectional studies that reported serum levels of anti-Dsg-1 and anti-Dsg-3 autoantibodies in OLP patients.

Results

Reticular-type OLP showed variable anti-Dsg-1 and anti-Dsg-3 autoantibody levels, with some patients testing positive while others were negative, indicating an inconsistent involvement of desmosomal autoimmunity in this milder form of the disease.

In erosive-type OLP, the presence of both autoantibodies indicated deeper epithelial damage and an active autoimmune response. Interestingly, ulcerative-type OLP consistently tested negative for both antibodies despite extensive epithelial breakdown, implying a potential shift from desmosomal autoimmunity to chronic inflammation–driven tissue destruction that could compromise human health.

Conclusion

These findings suggest a potential continuum in which reticular OLP may progress to erosive OLP, with Dsg-3 autoantibodies as a possible early marker of worsening pathology. Ulcerative OLP appears to represent a late-stage, inflammation-driven phenotype with minimal autoantibody involvement. Given the limited and heterogeneous data, these observations are preliminary and should be interpreted cautiously.

目的：针对桥粒蛋白的自身抗体，特别是桥粒蛋白1 （Dsg-1）和桥粒蛋白3 (Dsg-3)，在调节口腔扁平苔藓（OLP）疾病严重程度和上皮完整性方面的潜在作用仍然是一个越来越受关注的主题。不同OLP亚型的抗dsg -1和抗dsg -3自身抗体的不同模式可能与疾病进展相关。本综述研究了网状、糜烂和溃疡性OLP中循环抗dsg -1和抗dsg -3自身抗体的表达模式，以阐明它们在上皮破坏和疾病进展中的潜在作用。设计：按照PRISMA的指导方针，系统地检索了四个电子数据库。符合条件的研究包括报告OLP患者血清抗dsg -1和抗dsg -3自身抗体水平的病例报告、病例系列、病例对照和横断面研究。结果：网状型OLP显示抗dsg -1和抗dsg -3自身抗体水平变化，一些患者检测呈阳性，而另一些患者检测呈阴性，表明在这种较轻形式的疾病中，桥粒体自身免疫的参与不一致。在糜烂型OLP中，两种自身抗体的存在表明更深的上皮损伤和积极的自身免疫反应。有趣的是，尽管有广泛的上皮破坏，溃疡型OLP的两种抗体检测结果始终呈阴性，这意味着从桥粒体自身免疫到慢性炎症驱动的组织破坏的潜在转变，可能危及人类健康。结论：这些发现表明网状OLP可能发展为糜烂性OLP， Dsg-3自身抗体可能是病理恶化的早期标志。溃疡性OLP似乎代表了一种晚期，炎症驱动的表型，自身抗体参与最小。考虑到有限和异质性的数据，这些观察结果是初步的，应该谨慎解释。

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引用次数: 0

Assessment of nutrient canals in relation to jaw cysts, trabecular bone structure and dental regions: A CBCT study 评估营养管与颌骨囊肿、骨小梁结构和牙齿区域的关系：一项CBCT研究

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2025-10-17 DOI: 10.1016/j.archoralbio.2025.106433

Yasemin Merve Yılmaz , Defne Yalçın

Objective

To investigate the relationship of nutrient canals with jaw cysts, trabecular bone density, and dental regions using cone-beam computed tomography (CBCT).

Design

This retrospective study analyzed 120 CBCT images from 60 patients with unilateral jaw cysts. Nutrient canals were identified and localized on both the cyst-affected side and contralateral, unaffected side of the jaw for each patient. Alveolar trabecular structure was assessed by comparing Hounsfield Unit (HU) values between affected and unaffected sides. Statistical analyses included paired t-tests, Fisher’s exact test, and intra-observer reliability assessment.

Results

Nutrient canals were significantly more prevalent in cyst-affected jaw regions. Their presence was associated with areas exhibiting pericystic trabecular sclerosis, as reflected by elevated HU values. While nutrient canals were commonly observed in the anterior regions of both healthy and affected jaws, their presence in premolar and molar regions was restricted to cyst-affected areas.

Conclusions

Nutrient canals, typically found in the anterior jaws, appear to develop in posterior regions in association with jaw cysts, particularly in the presence of trabecular sclerosis. Their identification on CBCT scans is important for treatment planning and for minimizing potential surgical complications.

目的应用锥形束ct （cone-beam computed tomography， CBCT）研究营养管与颌骨囊肿、骨小梁骨密度及牙区之间的关系。本回顾性研究分析了60例单侧颌囊肿患者的120张CBCT图像。在每个患者的受囊肿影响的一侧和对侧未受影响的一侧颌骨上确定并定位营养管。通过比较患侧和未患侧的Hounsfield Unit （HU）值来评估肺泡小梁结构。统计分析包括配对t检验、Fisher精确检验和观察者内部信度评估。结果在患囊肿的颌区，营养管明显增多。它们的存在与表现为囊周小梁硬化的区域有关，这可以通过HU值升高来反映。虽然营养管通常在健康和患病颌骨的前区观察到，但它们在前磨牙和磨牙区域的存在仅限于囊肿影响区域。结论：在颌骨囊肿患者中，特别是在小梁硬化患者中，通常在前颚发现的三通管出现在下颌后部。在CBCT扫描上识别它们对于治疗计划和减少潜在的手术并发症非常重要。

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引用次数: 0

Erratum to “Role of Fusobacterium nucleatum in the pathogenesis of oral squamous cell carcinoma: A systematic review of molecular mechanisms and signalling pathways” [Archives of Oral Biology 179 (2025) 106369] “核梭杆菌在口腔鳞状细胞癌发病中的作用：分子机制和信号通路的系统综述”[口腔生物学档案179(2025)106369]。

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2025-10-17 DOI: 10.1016/j.archoralbio.2025.106408

Bara’a El-Dwaikat , Ikhlas El Karim , Daniel B. Longley , Paul N. Moynagh , Fionnuala T. Lundy

引用次数: 0

Ganoderic acid A attenuates Porphyromonas gingivalis-induced adhesion molecule expression in gingival fibroblasts and tissues via inhibition of NLRP6 inflammasome activation 灵芝酸A通过抑制NLRP6炎性小体激活，减弱牙龈卟啉单胞菌诱导的牙龈成纤维细胞和组织中的粘附分子表达。

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2025-10-15 DOI: 10.1016/j.archoralbio.2025.106429

Yuanbo Wang , Jianru Liu , Xiangying Ouyang , Wenyi Liu , Peiying Lyu , Shengnan Zhang , Jing Guo

Objectives

This study aimed to elucidate the regulatory effects and mechanisms of Ganoderic acid A (GAA) on Porphyromonas gingivalis (P. gingivalis)-induced expression of adhesion molecules in human gingival fibroblasts (hGFs) and periodontal tissues, focusing on NLRP6 inflammasome modulation.

Design

HGFs were stimulated with P. gingivalis strain W83. The expression levels of NLRP6, caspase-1, IL-1β,IL-18 and adhesion molecules ICAM-1 & VCAM-1 were analyzed by RT-PCR, Western blotting and ELISA, with or without GAA pretreatment. To further explore the regulatory role of GAA on the NLRP6 inflammasome, NLRP6 overexpression assays were performed in hGFs, followed by assessment of ICAM-1 and VCAM-1 expressions. Additionally, a ligature-induced periodontitis mice model was established to evaluate the effects of GAA on NLRP6 expression and inflammatory cell infiltration in periodontal tissues.

Results

GAA exhibited no cytotoxicity toward hGFs at low concentrations (within 16 μM). GAA pretreatment significantly inhibited P. gingivalis-induced activation of the NLRP6 inflammasome. Beyond IL-1β and IL-18, it also reduced ICAM-1 and VCAM-1 expression at both mRNA and protein levels. Overexpression of NLRP6 increased the expression of NLRP6 inflammasome components and adhesion molecules, whereas GAA treatment effectively suppressed these elevations. Furthermore, GAA administration markedly downregulated NLRP6 expression and attenuated inflammatory cell infiltration and periodontal inflammation in the mice periodontitis model.

Conclusions

GAA attenuates P. gingivalis-induced expression of adhesion molecules by inhibiting NLRP6 inflammasome activation in gingival fibroblasts and periodontal tissues, thereby alleviating inflammatory cell infiltration. These findings suggest the potential of GAA as a therapeutic agent for the management of periodontal inflammation.

目的：研究灵芝酸A （GAA）对牙龈卟啉单胞菌（P. gingivalis）诱导的人牙龈成纤维细胞（hGFs）和牙周组织中粘附分子表达的调控作用及其机制，重点研究NLRP6炎症小体的调控作用。设计：用牙龈假单胞菌菌株W83刺激hgf。采用RT-PCR、Western blotting和ELISA检测GAA预处理前后NLRP6、caspase-1、IL-1β、IL-18和粘附分子ICAM-1、VCAM-1的表达水平。为了进一步探讨GAA对NLRP6炎症小体的调节作用，我们在hgf中进行NLRP6过表达测定，随后评估ICAM-1和VCAM-1的表达。建立结扎性牙周炎小鼠模型，观察GAA对牙周组织NLRP6表达及炎症细胞浸润的影响。结果：低浓度（16 μM以内）GAA对hGFs无细胞毒性。GAA预处理可显著抑制牙龈假单胞菌诱导的NLRP6炎性体的活化。除IL-1β和IL-18外，它还能在mRNA和蛋白水平上降低ICAM-1和VCAM-1的表达。NLRP6的过表达增加了NLRP6炎症小体成分和粘附分子的表达，而GAA治疗有效地抑制了这些升高。此外，GAA可显著下调小鼠牙周炎模型中NLRP6的表达，减轻炎症细胞浸润和牙周炎症。结论：GAA通过抑制NLRP6炎症小体在牙龈成纤维细胞和牙周组织中的活化，减弱牙龈假单胞菌诱导的粘附分子表达，从而减轻炎症细胞浸润。这些发现提示了GAA作为牙周炎症治疗药物的潜力。

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引用次数: 0

Altered oral microbiome diversity in patients with oral candidiasis 口腔念珠菌病患者口腔微生物群多样性的改变

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2025-10-15 DOI: 10.1016/j.archoralbio.2025.106430

Ji Rak Kim , Jin-Seok Byun , Jae-Kwang Jung , Su-Hyung Hong , Heon-Jin Lee

Objective

Oral candidiasis is a common opportunistic infection caused by Candida albicans, particularly in individuals with local or systemic risk factors. This study aimed to investigate how antifungal therapy affects the composition of the oral bacterial microbiome.

Design

Unstimulated saliva samples were collected from ten patients diagnosed with acute pseudomembranous oral candidiasis before and after fluconazole treatment. Microbiome profiles were assessed using 16S rRNA gene sequencing. Quantitative PCR was performed to validate changes in specific bacterial species.

Results

Alpha diversity did not change significantly, whereas beta-diversity analyses indicated modest compositional shifts. Antifungal therapy was associated with an increase in Streptococcus salivarius, a commensal linked to mucosal health. The signal was confirmed by species-specific qPCR in paired samples.

Conclusions

Fluconazole treatment for oral candidiasis induces modest shifts in the oral bacterial community, particularly increasing the abundance of S. salivarius. These changes may reflect partial recovery of microbial homeostasis, supporting the role of microbiome monitoring and probiotic approaches in post-treatment care.

目的口腔念珠菌病是一种常见的由白色念珠菌引起的机会性感染，特别是在有局部或全身危险因素的个体中。本研究旨在探讨抗真菌治疗如何影响口腔细菌微生物组的组成。设计收集10例急性假膜性口腔念珠菌病患者氟康唑治疗前后的非刺激唾液样本。采用16S rRNA基因测序评估微生物组谱。采用定量PCR验证特定菌种的变化。结果α - α多样性变化不显著，β - α多样性变化不大。抗真菌治疗与唾液链球菌的增加有关，这是一种与粘膜健康相关的共生菌。该信号在配对样本中被物种特异性qPCR证实。结论氟康唑治疗口腔念珠菌病可引起口腔细菌群落的适度变化，特别是增加了唾液链球菌的丰度。这些变化可能反映了微生物稳态的部分恢复，支持微生物组监测和益生菌方法在治疗后护理中的作用。

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引用次数: 0

Bacterial extracellular vesicles from Porphyromonas gingivalis – proteomic profile and influence on oral fibroblasts 牙龈卟啉单胞菌的细菌胞外囊泡-蛋白质组学特征及其对口腔成纤维细胞的影响。

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2025-10-15 DOI: 10.1016/j.archoralbio.2025.106424

Helene Rygvold Haugsten , Anne Karin Kristoffersen , Morten Enersen , Bernd Thiede , Tine M. Søland , Trude M. Haug , Hilde Kanli Galtung

Objectives

In this study, we aimed to explore the protein content of bacterial extracellular vesicles (bEVs) from three strains of Porphyromonas gingivalis, an anaerobic gram-negative bacterium closely associated with periodontitis. Additionally, we aimed to investigate the effects of bEV uptake on human oral fibroblasts, to better understand the role of these vesicles in the pathogenesis of periodontitis.

Design

Liquid chromatography-mass spectrometry was performed on bEVs from three strains of P. gingivalis with different virulence: ATCC 33277, A7A1–28, and W83. Three key proteins associated with virulence were validated using dot blot. Proliferation and viability of human oral fibroblasts after incubation with bEVs were assessed at different timepoints and different concentrations of bEVs.

Results

A total of 307 proteins were identified in the bEVs from the three strains of P. gingivalis. Among those were several relevant for virulence, for example gingipains, fimbriae, and peptidyl-arginine deiminase (PPAD). When oral fibroblasts were exposed to bEVs from A7A1–28 or W83, proliferation was significantly affected after 12 and 24 h at one bEV concentration. Furthermore, viability was significantly affected by bEVs from W83 at several timepoints and bEV concentrations. bEVs from ATCC 33277 did not induce any significant changes.

Conclusion

Several proteins associated with virulence were detected in bEVs from all three strains of P. gingivalis, with varying abundance. Uptake of these bEVs can influence human cells, as exemplified by changes in viability and proliferation.

目的：在本研究中，我们旨在探讨三株牙龈卟啉单胞菌（一种与牙周炎密切相关的革兰氏阴性厌氧菌）的细菌细胞外囊泡（bEVs）的蛋白质含量。此外，我们旨在研究bEV摄取对人口腔成纤维细胞的影响，以更好地了解这些囊泡在牙周炎发病机制中的作用。设计：采用液相色谱-质谱法对三株不同毒力的牙龈假单胞菌ATCC 33277、A7A1-28和W83的bev进行分析。3个与毒力相关的关键蛋白采用点印迹法进行了验证。研究了不同时间点、不同浓度bev对人口腔成纤维细胞增殖和活力的影响。结果：从3株牙龈假单胞菌的bev中共鉴定出307个蛋白。其中有几个与毒力有关，例如牙龈痛、菌毛和肽精氨酸脱亚胺酶（PPAD）。当口腔成纤维细胞暴露于A7A1-28或W83的bEV时，在一个bEV浓度下，12和24 h后，增殖受到显著影响。此外，在不同时间点和不同浓度下，W83的bEV对细胞活力有显著影响。ATCC 33277的纯电动汽车没有引起任何显著的变化。结论：在三株牙龈假单胞菌的bev中均检测到几种与毒力相关的蛋白，且丰度不同。这些bev的摄取可以影响人类细胞，如活力和增殖的变化。

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引用次数: 0

TWF1 promotes tumor progression in head and neck squamous cell carcinoma via AKT phosphorylation TWF1通过AKT磷酸化促进头颈部鳞状细胞癌的肿瘤进展。

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2025-10-14 DOI: 10.1016/j.archoralbio.2025.106425

Yingshun Yang , Kaicheng Yang , Shixiong Peng , Shasha Man , He Chen

Objective

To investigate the molecular mechanisms underlying how Twinfilin actin-binding protein 1 (TWF1) drives the malignant progression of head and neck squamous cell carcinoma (HNSCC).

Design

A pan-cancer analysis of TCGA datasets was performed to evaluate TWF1 expression and prognostic significance, followed by validation using quantitative real-time PCR (qPCR) and immunohistochemistry (IHC) in clinical HNSCC specimens. Functional assays (CCK-8, colony formation, wound healing, and Transwell invasion) were conducted following siRNA-mediated TWF1 knockdown in SCC9 cells. Additionally, the same functional assays were conducted in an HSC3 cell model overexpressing TWF1 to further evaluate its oncogenic function. The involvement of the AKT signaling pathway was examined by Western blot and validated through rescue experiments with the AKT activator SC79.

Results

TWF1 was found to be upregulated in 13 cancer types, including HNSCC. Elevated TWF1 expression, advanced T stage, and lymph node metastasis were identified as independent prognostic indicators in HNSCC. qPCR confirmed increased TWF1 expression, and IHC showed higher TWF1 protein levels in HNSCC tissues. TWF1 knockdown suppressed SCC9 cell proliferation, migration, and invasion, and reduced phosphorylated AKT levels. Conversely, TWF1 overexpression in HSC3 cells promoted proliferation, migration, and invasion while enhancing AKT phosphorylation. SC79 treatment partially reversed the malignant phenotypes suppressed by TWF1 knockdown, supporting TWF1’s regulatory role through AKT phosphorylation.

Conclusion

TWF1 acts as an oncogenic driver in HNSCC, promoting tumor progression through the AKT signaling pathway. It may serve as a potential therapeutic target in precision medicine for HNSCC.

目的：探讨Twinfilin actin-binding protein 1 （TWF1）驱动头颈部鳞状细胞癌（HNSCC）恶性进展的分子机制。设计：对TCGA数据集进行泛癌分析，评估TWF1的表达和预后意义，随后使用定量实时PCR （qPCR）和免疫组织化学（IHC）对临床HNSCC标本进行验证。在SCC9细胞中sirna介导的TWF1敲除后进行功能测定（CCK-8、菌落形成、伤口愈合和Transwell侵袭）。此外，在过表达TWF1的HSC3细胞模型中进行了相同的功能测定，以进一步评估其致癌功能。通过Western blot检测AKT信号通路的参与情况，并通过AKT激活剂SC79的拯救实验进行验证。结果：在包括HNSCC在内的13种癌症类型中发现TWF1表达上调。TWF1表达升高、T分期晚期和淋巴结转移被确定为HNSCC的独立预后指标。qPCR证实TWF1表达升高，IHC显示HNSCC组织中TWF1蛋白水平升高。TWF1敲除抑制SCC9细胞增殖、迁移和侵袭，降低磷酸化AKT水平。相反，在HSC3细胞中，TWF1过表达促进增殖、迁移和侵袭，同时增强AKT磷酸化。SC79治疗部分逆转了TWF1敲低抑制的恶性表型，支持了TWF1通过AKT磷酸化的调节作用。结论：TWF1在HNSCC中发挥致癌驱动作用，通过AKT信号通路促进肿瘤进展。它可能成为HNSCC精准医学的潜在治疗靶点。

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引用次数: 0

Topical effect of polysaccharide cashew gum on the mitigation of dentin erosion progression under in vitro supraesophageal reflux simulation 腰果多糖对体外食管上反流模拟下牙本质侵蚀进展的局部影响

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2025-10-14 DOI: 10.1016/j.archoralbio.2025.106427

Thaysa Fernandes Pinto Mendes , Adrianne Caroline Pereira dos Santos Lima , Eva Aline Costa Cutrim , Paulo Sérgio de Araújo Sousa , Durcilene Alves da Silva , Lucas Antonio Duarte Nicolau , Leily Macedo Firoozmand

Objective

To evaluate, in vitro, the topical effect of polysaccharide cashew gum (PLS-cg) on dentine subjected to erosive cycles that simulate refluxate contact in the oral cavity.

Design

Human dentine blocks were prepared, standardized, and randomly assigned to one of three treatment groups (n = 10): control group (C) without treatment, commercial dental varnish (CDV) 5 % NaF fluoride varnish, and PLS-cg extract (experimental group). The baseline enamel superficial microhardness (HK) and roughness (Ra) were assessed. The dentin was subjected to erosive cycles (6 × 5 min/day for 9 days) using a hydrochloric acid–pepsin solution (pH 2; 0.75-mg/mL pepsin). Surface roughness (Ra), microhardness (HK), percentage surface hardness loss (%SHL), and topographic analysis with scanning electron microscopy (SEM) were used to evaluate the protective heteropolysaccharide effect. Predictive molecular docking simulations were used to investigate possible interactions between PLS-cg and metalloproteinases (MMP2 and MMP9). The data were analyzed with two-way analysis of variance, t test, Pearson’s correlation, and Tukey’s test (p < 0.05).

Results

PLS-cg preserved HK significantly better than the control group (p < 0.001), with the lowest %SHL (-14.04 ± 8.22) and minimal Ra changes (0.124 ± 0.005). Pearson’s correlation between Ra and %SHL was significant and positive (0.7740, p < 0.001). SEM analysis revealed more prominent dentinal tubule openings in the control group, whereas CDV and PLS-cg showed tubule obliteration. Molecular docking identified possible interactions between PLS-cg and MMPs.

Conclusion

The findings indicate that PLS-cg may reduce dentine erosion caused by supraesophageal manifestations of gastroesophageal reflux disease.

目的：体外评价腰果多糖（PLS-cg）对牙本质在模拟口腔内反流接触的侵蚀循环中的局部作用。设计：将人牙本质块制备、标准化并随机分配到三个治疗组（n = 10）中的一个：对照组(C)未治疗，商用牙清漆（CDV） 5 % NaF氟化物清漆和PLS-cg提取物（实验组）。评估基线牙釉质表面显微硬度（HK）和粗糙度（Ra）。使用盐酸-胃蛋白酶溶液（pH 2； 0.75 mg/mL胃蛋白酶）对牙本质进行侵蚀循环（6 × 5 分钟/天，持续9天）。采用表面粗糙度（Ra）、显微硬度（HK）、表面硬度损失率（%SHL）和扫描电镜形貌分析（SEM）来评价杂多糖的保护作用。预测分子对接模拟用于研究PLS-cg与金属蛋白酶（MMP2和MMP9）之间可能的相互作用。对数据进行双向方差分析、t检验、Pearson相关和Tukey检验（p ）结果：PLS-cg保存HK显著优于对照组（p ）结论：PLS-cg可减少胃食管反流病食管上表现引起的牙本质糜烂。

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