Archives of oral biology最新文献_第7页

E-cigarette exposure increases caries risk and modifies dental surface in an in vitro model 在体外模型中，接触电子烟会增加龋齿风险并改变牙齿表面。

IF 2.2 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2024-11-14 DOI: 10.1016/j.archoralbio.2024.106128

Aline Evangelista Souza-Gabriel, Vitoria Leite Paschoini-Costa, Manoel Damião Sousa-Neto, Alice Corrêa Silva-Sousa

Objective

Electronic cigarettes have become increasingly popular and can deliver nicotine at levels comparable to traditional tobacco cigarettes. However, the potential adverse effects of these alternative smoking devices on dental health remain uncertain. This study investigates changes in the cariogenic potential of tooth surfaces and analyzes alterations in the chemical composition of aerosols generated from sweet-flavored e-liquids used in electronic cigarettes.

Design

Smoking was simulated using an electronic-cigarette testing machine. Eighty specimens of enamel, dentin and root dentin were divided in two groups according smoking: pre-smoke and post-smoke. The response variables were Microhardness (n=10), SEM (n=5) and FTIR (n=5). E-liquid was analyzed pre-smoke and post-smoke by HPLC-UV/Vis. Data were analyzed by one-way ANOVA and Tukey's tests, with a significance level of 5 %. SEM, FTIR and HPLC-UV/Vis data were qualitatively analyzed.

Results

Enamel exhibited higher microhardness values before and after smoking, with all substrates showing significant microhardness reduction after smoking. All dentin specimens presented standard dentinal tubules, and post-smoke enamel prisms appeared disorganized with various orientations. The specimens’ chemical constituents remained stable. Dentin and root dentin post-smoke, carbonate band intensity decreased. There was a thermal degradation of e-liquid products and the formation of new compounds post-vaporization.

Conclusions

E-cigarette smoking reduces the microhardness of enamel, dentin, and root dentin, alters enamel morphology, induces chemical interactions between e-liquid and tooth tissues, and may increase the risk of cariogenic potential.

目的：电子香烟越来越受欢迎，其尼古丁含量与传统烟草香烟相当。然而，这些替代吸烟设备对牙齿健康的潜在不良影响仍不确定。本研究调查了牙齿表面致龋可能性的变化，并分析了电子烟中使用的甜味电子烟液所产生的气溶胶化学成分的变化：设计：使用电子烟测试机模拟吸烟。根据吸烟情况将 80 个牙釉质、牙本质和根部牙本质标本分为两组：吸烟前和吸烟后。响应变量为显微硬度（10 个）、扫描电镜（5 个）和傅立叶变换红外光谱（5 个）。通过 HPLC-UV/Vis 对吸烟前和吸烟后的电子液体进行分析。数据采用单因子方差分析和 Tukey 检验，显著性水平为 5%。对扫描电镜、傅立叶变换红外光谱和 HPLC-UV/Vis 数据进行了定性分析：吸烟前后珐琅质的显微硬度值较高，吸烟后所有基质的显微硬度都显著降低。所有牙本质样本都呈现出标准的牙本质小管，而吸烟后的珐琅质棱柱显得杂乱无章，方向各异。样本的化学成分保持稳定。烟熏后的牙本质和根部牙本质碳酸盐带强度降低。电子烟液产品出现热降解，并在蒸发后形成新的化合物：吸电子烟会降低牙釉质、牙本质和牙根的微硬度，改变牙釉质形态，诱发电子烟液与牙齿组织之间的化学作用，并可能增加致龋风险。

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引用次数: 0

Histopathological characterization of mandibular condyles in four temporomandibular joint osteoarthritis mouse models 四种颞下颌关节骨关节炎小鼠模型下颌骨髁状突的组织病理学特征。

IF 2.2 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2024-11-14 DOI: 10.1016/j.archoralbio.2024.106129

Ludan Xing , Xin Qi , Jiayan Wu , Yuan Liu , Liyuan Zhang , Beizhan Jiang

Objective

Temporomandibular joint osteoarthritis (TMJOA) has been modeled in different ways with a lack of uniformity. We aimed to investigate four TMJOA mouse models and assess histopathological changes in condyles, which could assist in the selection of animal models in further TMJOA-related studies.

Design

Four TMJOA mouse models were established, including unilateral hyperocclusion, discectomy, monosodium iodoacetate injection and aged model. Bilateral condyles were collected at different time points. The condylar alterations were analyzed by Micro-CT, Hematoxylin and eosin staining, Toluidine blue staining, Safranin O staining, Trap staining, immunofluorescence staining, immunohistochemistry staining and quantitative polymerase chain reaction.

Results

Radiographic and histopathological analysis indicated that all four methods could cause condylar degeneration successfully. Differences in morphologic and histologic changes were found among four models. The hyperocclusion model was time-dependent and the lesions got worse over time. Discectomy model presented obvious damage of cartilage and subchondral bone. Injected model showed severe inflammation and chondrocyte hypertrophy. The aged model was characterized by decreased of proteoglycan and osteolysis.

Conclusions

The four methods had different characteristics and applicability. The harvest time affected the degree of cartilage degradation. Hyperocclusion was suited to explore the early-stage of TMJOA. Discectomy present advantages in investigating the long-term restoration of cartilage and subchondral bone. Monosodium iodoacetate-injection was appropriate for screening the agents for inflammatory relief. The aged model more naturally facilitated discovering underlying mechanisms in primary TMJOA. Unilateral modeling methods could initiate contralateral condylar alterations. The TMJOA models should be selected based on experimental requirements and applicability of each model.

目的：颞下颌关节骨关节炎（TMJOA）的建模方式各不相同，缺乏统一性。我们旨在研究四种颞下颌关节骨关节炎小鼠模型，并评估髁突的组织病理学变化，这有助于在进一步的颞下颌关节骨关节炎相关研究中选择动物模型：设计：建立了四种 TMJOA 小鼠模型，包括单侧咬合过度模型、椎间盘切除模型、碘乙酸钠注射模型和老年模型。在不同时间点采集双侧髁突。通过显微 CT、苏木精和伊红染色、甲苯胺蓝染色、沙夫林 O 染色、Trap 染色、免疫荧光染色、免疫组化染色和定量聚合酶链反应分析髁突的变化：结果：放射学和组织病理学分析表明，这四种方法都能成功导致髁突变性。四种模型的形态学和组织病理学变化存在差异。超咬合模型具有时间依赖性，病变随着时间的推移而加重。椎间盘切除模型的软骨和软骨下骨明显受损。注射模型表现出严重的炎症和软骨细胞肥大。老化模型的特点是蛋白多糖减少和骨溶解：结论：四种方法具有不同的特点和适用性。结论：四种方法具有不同的特点和适用性，采集时间会影响软骨退化的程度。超咬合适合于研究颞下颌关节损伤的早期阶段。椎间盘切除术在研究软骨和软骨下骨的长期恢复方面具有优势。碘乙酸钠注射适合用于筛选缓解炎症的药物。老年模型更自然地有助于发现原发性颞下颌关节扭伤的潜在机制。单侧建模方法可引发对侧髁突改变。应根据实验要求和每种模型的适用性选择颞下颌关节紊乱模型。

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引用次数: 0

Cyclosorus terminans extract mitigates submandibular gland changes associated with high-fat diet consumption in male rats 旋覆花提取物可减轻雄性大鼠下颌下腺因摄入高脂肪饮食而发生的变化。

IF 2.2 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2024-11-13 DOI: 10.1016/j.archoralbio.2024.106127

Nopphakhun Lungruammit , Hiranya Pintana , Wasana Pratchayasakul , Sujinda Songtrai , Sireewan Kaewsuwan , Jitjiroj Ittichaichareon , Nipon Chattipakorn , Siriporn C. Chattipakorn

Objectives

To investigate whether the prophylactic effect of Cyclosorus terminans extract mitigates metabolic impairment and submandibular gland changes, as indicated by increased aquaporin5 expression, decreased fibrosis, oxidative stress and inflammation, improved mitochondrial homeostasis/dynamics, and decreased cell death in the submandibular glands of high-fat diet (HFD)-feeding rats.

Methods

Thirty-two male Wistar rats were assigned to either a normal diet (ND) as control rats (n=8) or a HFD (n=24) for 12 weeks. The HFD-treated rats were divided into 3 subgroups to receive either: 1) vehicle (HDV), 2) Cyclosorus terminans at a dose of 100 mg/kg/d (HF100), or 3) Cyclosorus terminans at a dose of 200 mg/kg/d (HF200). At week 13, metabolic parameters, systemic oxidative stress, and submandibular gland parameters were assessed.

Results

Twelve weeks of HFD-feeding rats induced obese-insulin resistance and submandibular gland changes. Both HF100- and HF200-treated groups improved metabolic parameters and prevented gland changes by reducing fibrosis (TGF-β and p-38), malondialdehyde levels, inflammation (TNF-α, NF-κB, and Ifng), and cell death markers (Caspase 3, GSDMD, and MLKL). Both treatments supported balanced mitochondrial homeostasis/dynamics, as indicated by regulating related genes (Cpt1b, Ndufb8, Mfn1, Mfn2, Opa1, and Dnm1l). However, only the HF200-treated rats restored aquaporin-5 and antioxidants (SOD2 and GPX4) expression to control levels.

Conclusions

Cyclosorus terminans mitigates metabolic disturbances and submandibular gland changes in HFD-feeding rats. The high dose was more effective, improving gland function by increasing aquaporin5 and antioxidants. These results suggest Cyclosorus terminans may be a promising therapeutic for metabolic disturbances and submandibular gland changes in obese-insulin resistant patients.

目的：研究旋覆花提取物的预防作用是否能减轻代谢损伤和下颌下腺的变化：研究高脂饮食（HFD）喂养大鼠颌下腺的代谢损伤和颌下腺变化（表现为水囊蛋白5表达增加，纤维化、氧化应激和炎症减少，线粒体稳态/动力学改善，细胞死亡减少）是否能减轻旋覆花提取物的预防作用：32只雄性Wistar大鼠被分配到正常饮食（ND）对照组（8只）或高脂饮食对照组（24只），为期12周。经高密度脂蛋白膳食处理的大鼠被分为 3 个亚组，分别接受以下两种药物治疗：1）药物（HDV）；2）药物（HDV）；3）药物（HDV）：1）载体（HDV）；2）剂量为 100 毫克/千克/天（HF100）或 3）剂量为 200 毫克/千克/天（HF200）的旋覆花。第13周时，对代谢参数、全身氧化应激和颌下腺参数进行评估：结果：喂食高氟酸膳食 12 周的大鼠诱发了肥胖-胰岛素抵抗和颌下腺变化。经 HF100 和 HF200 处理的组均可通过降低纤维化（TGF-β 和 p-38）、丙二醛水平、炎症（TNF-α、NF-κB 和 Ifng）和细胞死亡标志物（Caspase 3、GSDMD 和 MLKL）来改善代谢参数和预防腺体变化。两种处理方法都支持线粒体平衡/动力学，这体现在对相关基因（Cpt1b、Ndufb8、Mfn1、Mfn2、Opa1 和 Dnm1l）的调节上。然而，只有经 HF200 处理的大鼠的水蒸发素-5 和抗氧化剂（SOD2 和 GPX4）表达恢复到控制水平：结论：旋覆花可减轻高纤维食物喂养大鼠的代谢紊乱和颌下腺变化。高剂量更有效，可通过增加水蒸发蛋白5和抗氧化剂来改善腺体功能。这些结果表明，旋覆花可作为肥胖-胰岛素抵抗患者代谢紊乱和下颌下腺变化的治疗药物。

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引用次数: 0

Measurement of the root surface area in rat molars through three-dimensional modeling 通过三维建模测量大鼠臼齿的根表面积。

IF 2.2 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2024-11-13 DOI: 10.1016/j.archoralbio.2024.106132

Yuanyuan Wei , Danyuan Huang , Shuo Chen , Yukun Jiang , Kuan Yang , Zhiai Hu , Hui Li , Shujuan Zou , Yuyu Li

Objectives

Rats are used as animal models for basic and applied research related to orthodontic tooth movement (OTM). The magnitude of mechanical force in OTM rat models mainly depends on the supporting capability of the periodontal ligament (PDL), which is highly associated with the root surface area (RSA). But the size of rat RSA remains unknown, which is the reason why there are still debates on the magnitude of mechanical force in OTM rat models. This study aimed to explore a method for measuring the RSA in rat molars.

Design

The maxillary and mandibular samples of rats were scanned by Micro-CT to generate three-dimensional (3D) images, followed by 3D reconstruction of every molar through Mimics Medical 21.0. Geomagic Wrap 2021 and Unigraphics NX 12.0 were utilized to smooth teeth surface and mark the cementoenamel junction (CEJ). Finally, the RSA in rat molars was measured.

Results

The results showed that for the six-, eight-, or ten-week-old rats, the average RSA of maxillary first, second, and third molars was 25.90 ± 2.29 mm², 15.92 ± 2.14 mm², and 10.34 ± 1.94 mm². The RSA of mandibular first, second, and third molars was 27.03 ± 2.63 mm², 17.16 ± 1.61 mm², and 11.39 ± 2.13 mm².

Conclusions

Through 3D modelling, we provided data of rat RSA, and observed the trend of increasing RSA mean values with age. These data are pivotal for determining the magnitude of mechanical force required to move rat molars, especially when conducting research related to OTM using rat models.

目的：大鼠被用作与正畸牙齿移动（OTM）相关的基础研究和应用研究的动物模型。OTM 大鼠模型中机械力的大小主要取决于牙周韧带（PDL）的支持能力，而牙周韧带的支持能力与牙根表面积（RSA）高度相关。但大鼠 RSA 的大小仍然未知，这也是对 OTM 大鼠模型中机械力大小仍存在争议的原因。本研究旨在探索一种测量大鼠磨牙 RSA 的方法：通过 Micro-CT 扫描大鼠的上颌和下颌样本，生成三维图像，然后通过 Mimics Medical 21.0 对每颗臼齿进行三维重建。Geomagic Wrap 2021 和 Unigraphics NX 12.0 用于平滑牙齿表面和标记牙釉质交界处（CEJ）。最后，测量了大鼠臼齿的 RSA：结果显示，六周龄、八周龄和十周龄大鼠上颌第一、第二和第三磨牙的平均 RSA 分别为 25.90 ± 2.29 mm2、15.92 ± 2.14 mm2 和 10.34 ± 1.94 mm2。下颌第一、第二和第三磨牙的 RSA 分别为 27.03 ± 2.63 mm2、17.16 ± 1.61 mm2 和 11.39 ± 2.13 mm2：通过三维建模，我们提供了大鼠 RSA 的数据，并观察到 RSA 平均值随年龄增长而增加的趋势。这些数据对于确定移动大鼠臼齿所需的机械力大小至关重要，尤其是在使用大鼠模型进行 OTM 相关研究时。

{"title":"Measurement of the root surface area in rat molars through three-dimensional modeling","authors":"Yuanyuan Wei , Danyuan Huang , Shuo Chen , Yukun Jiang , Kuan Yang , Zhiai Hu , Hui Li , Shujuan Zou , Yuyu Li","doi":"10.1016/j.archoralbio.2024.106132","DOIUrl":"10.1016/j.archoralbio.2024.106132","url":null,"abstract":"<div><h3>Objectives</h3><div>Rats are used as animal models for basic and applied research related to orthodontic tooth movement (OTM). The magnitude of mechanical force in OTM rat models mainly depends on the supporting capability of the periodontal ligament (PDL), which is highly associated with the root surface area (RSA). But the size of rat RSA remains unknown, which is the reason why there are still debates on the magnitude of mechanical force in OTM rat models. This study aimed to explore a method for measuring the RSA in rat molars.</div></div><div><h3>Design</h3><div>The maxillary and mandibular samples of rats were scanned by Micro-CT to generate three-dimensional (3D) images, followed by 3D reconstruction of every molar through Mimics Medical 21.0. Geomagic Wrap 2021 and Unigraphics NX 12.0 were utilized to smooth teeth surface and mark the cementoenamel junction (CEJ). Finally, the RSA in rat molars was measured.</div></div><div><h3>Results</h3><div>The results showed that for the six-, eight-, or ten-week-old rats, the average RSA of maxillary first, second, and third molars was 25.90 ± 2.29 mm<sup>2</sup>, 15.92 ± 2.14 mm<sup>2</sup>, and 10.34 ± 1.94 mm<sup>2</sup>. The RSA of mandibular first, second, and third molars was 27.03 ± 2.63 mm<sup>2</sup>, 17.16 ± 1.61 mm<sup>2</sup>, and 11.39 ± 2.13 mm<sup>2</sup>.</div></div><div><h3>Conclusions</h3><div>Through 3D modelling, we provided data of rat RSA, and observed the trend of increasing RSA mean values with age. These data are pivotal for determining the magnitude of mechanical force required to move rat molars, especially when conducting research related to OTM using rat models.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"170 ","pages":"Article 106132"},"PeriodicalIF":2.2,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142678113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dental plaque as an extra-gastric reservoir of Helicobacter pylori: A systematic review and meta-analysis 牙菌斑是幽门螺旋杆菌的胃外储库：系统回顾和荟萃分析

IF 2.2 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2024-11-10 DOI: 10.1016/j.archoralbio.2024.106126

Pradeep S. Anand , Kavitha P. Kamath , Aravind P. Gandhi , Muhammad Aaqib Shamim , Bijaya K. Padhi , Sakshi Das

Objective

This systematic review and meta-analysis (SRMA) aimed to determine whether the presence of H. pylori in dental plaque is associated with gastric H. pylori infection.

Design

Search for the relevant literature was done in various databases: PubMed, Embase, Web of Science and Cochrane till September 21, 2023. The studies were screened for outcome of interest i.e. gastric H. pylori infection and exposure of interest i.e. H. pylori positivity in dental plaque. The pooled results of the study outcomes were evaluated using Odds Ratio (OR), accompanied by a 95 % confidence interval (CI). To evaluate the heterogeneity among studies, I² statistics were utilized, with an I² exceeding 50 % indicating a significant to high variation. In cases where I² was greater than 50 %, a random-effects model (specifically, the Der Simonian and Laird method) was employed.

Results

Data from 27 observational studies and 2408 participants were included. The pooled OR of the H. pylori positivity in dental plaque among the patients with H. pylori positivity in stomach was 3.80 (95 % CI 2.24; 6.43), with high heterogeneity (I²= 69 %, p<0.01). Sensitivity analysis after removing the outliers reduced the heterogeneity significantly (I²=22 %, p=0.16). Meta-regression revealed that the strength of association did not vary according to the year of study or the sample size included in the studies. Overall certainty of the evidence was low.

Conclusions

The present meta-analysis showed that the presence of gastric H. pylori infection was higher among patients with H. pylori in dental plaque compared to patients without H. pylori in dental plaque. Presence of H. pylori infection in dental plaque can have implications in the management of H. pylori infection as dental plaque can serve as a reservoir from which the microorganism can recolonize the gastric mucosa.

目标本系统综述和荟萃分析（SRMA）旨在确定牙菌斑中幽门螺杆菌的存在是否与胃幽门螺杆菌感染有关：截至 2023 年 9 月 21 日，在 PubMed、Embase、Web of Science 和 Cochrane 等多个数据库中搜索了相关文献。对研究结果（即胃幽门螺杆菌感染）和研究暴露（即牙菌斑中幽门螺杆菌阳性）进行了筛选。研究结果的汇集结果采用比值比 (OR) 进行评估，并附有 95% 的置信区间 (CI)。为了评估研究之间的异质性，我们使用了 I2 统计量，I2 超过 50% 表示差异显著或较大。如果 I2 大于 50%，则采用随机效应模型（特别是 Der Simonian 和 Laird 方法）。在胃幽门螺杆菌阳性的患者中，牙菌斑中幽门螺杆菌阳性的汇总 OR 值为 3.80 (95 % CI 2.24; 6.43)，异质性较高 (I2= 69 %, p<0.01)。剔除异常值后的敏感性分析显著降低了异质性（I2=22 %，p=0.16）。元回归显示，相关性的强弱并不因研究年份或研究样本大小而异。结论本荟萃分析显示，与牙菌斑中未检出幽门螺杆菌的患者相比，牙菌斑中检出幽门螺杆菌的患者胃幽门螺杆菌感染率更高。牙菌斑中存在幽门螺杆菌感染可能会对幽门螺杆菌感染的治疗产生影响，因为牙菌斑可以作为幽门螺杆菌的储藏库，幽门螺杆菌可以从牙菌斑中重新定植到胃粘膜中。

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引用次数: 0

An overview of the microbiota of the oral cavity of humans and non-human primates with periodontal disease: Current issues and perspectives 人类和非人类灵长类动物牙周病口腔微生物群综述：当前问题和观点。

IF 2.2 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2024-11-10 DOI: 10.1016/j.archoralbio.2024.106121

Bruno Pires Miranda , Amanda Figueira da Silva , Júlia de Castro Ascenção , Helena Lúcia Carneiro Santos

Objective

To provide a comprehensive summary of the available evidence on the oral microbiota of humans and non-human primates about the etiology of periodontal disease.

Design

An integrative literature review was conducted on 398 clinical and observational articles published between 2010 and 2024 using searches in the MEDLINE/PubMed, Virtual Health Library, and SciELO databases. After the screening, eligibility, data extraction, and methodological quality assessment, 21 studies were selected.

Results

The results, which reveal striking similarities between the pathogens involved in periodontal disease in humans and NHPs, confirm the potential of NHPs as research models and inspire further research in this area.

Conclusion

According to these studies, Actinomyces spp., Aggregatibacter actinomycetemcomitans, Campylobacter rectus, Capnocytophaga spp., Eubacterium spp., Filifactor alocis, Fusobacterium spp., Leptotrichia spp., Neisseria mucosa, Parvimonas micra, Porphyromonas spp., Prevotella spp., Selenomonas spp., Streptococcus spp., Treponema spp., Tannerella spp., Veillonella parvula, were the genus and/or species of bacteria found in humans. On the other hand, Aggregatibacter acinomycetemcomitans, Campylobacter rectus, Desulfobulbus spp., Dialister invisus, Eikenella corrodens, Filifactor alocis, Fusobacterium spp., Parvimonas micra, Porphyromonas spp., Prevotella spp., Staphylococcus spp., Streptococcus spp., Treponema spp., Tannerella spp., Veillonella spp., were the most reported in NHPs. No study in non-human primates reported the presence of protozoa, unlike studies in humans that reported the presence of Entamoeba gingivalis and Trichomonas tenax. However, its role in periodontal disease still needs to be elucidated, despite the strong association mainly with severe periodontal disease where protozoa are observed in injured tissues.

目的：全面总结人类和非人类灵长类动物口腔微生物群与牙周病病因的关系。设计：通过检索MEDLINE/PubMed、虚拟健康图书馆和SciELO数据库，对2010年至2024年间发表的398篇临床和观察性文章进行综合文献综述。经过筛选、入选、数据提取和方法学质量评估，共选择21项研究。结果：这些结果揭示了人类牙周病病原体与NHPs之间惊人的相似性，证实了NHPs作为研究模型的潜力，并启发了该领域的进一步研究。结论：根据这些研究，放线菌、放线菌聚合菌、直弯曲菌、嗜糖菌、真杆菌、嗜酸丝状杆菌、梭杆菌、细毛纤毛菌、粘膜奈瑟菌、微细小单胞菌、卟啉单胞菌、普雷沃菌、硒单胞菌、链球菌、密螺旋体、坦纳菌、小叶细孔菌是在人体中发现的细菌属或种。另方面，NHPs中报告最多的是放线菌聚集菌、直弯曲菌、Desulfobulbus、Dialister invisus、Eikenella crodens、Filifactor alocis、Fusobacterium、Parvimonas micra、Porphyromonas spp、Prevotella spp、Staphylococcus spp、Streptococcus spp、Treponema spp、Tannerella spp、Veillonella spp。在非人类灵长类动物中没有研究报告原生动物的存在，而在人类研究中报告了牙龈内阿米巴和毛滴虫的存在。然而，它在牙周病中的作用仍然需要阐明，尽管它主要与严重的牙周病密切相关，在受伤的组织中观察到原生动物。

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引用次数: 0

Effects of loxoprofen on impaired water-evoked swallows in a pharyngitis rat model 洛索洛芬对咽炎大鼠模型水诱发吞咽功能受损的影响

IF 2.2 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2024-11-02 DOI: 10.1016/j.archoralbio.2024.106125

Mari Fukuzaki , Chihiro Nakatomi , Sayaka Kubo , Tomoki Shimada , Keiko Tsuji , Chia-Chien Hsu , Tatsuo Kawamoto , Kentaro Ono

Objective

The objective of this study was to investigate the effects of nonsteroidal anti-inflammatory drugs (NSAIDs; e.g., loxoprofen) on pharyngitis-induced dysphagia in rats.

Design

A pharyngitis rat model was generated by topical application of acetic acid to the laryngopharyngeal region. Changes in water swallowing were evaluated by endoscopic observation over time. A histological analysis of the laryngopharyngeal mucosa was performed. The effects of loxoprofen, antibacterial drugs, and transient receptor potential (TRP) channel inhibitors were investigated in model rats.

Results

After acetic acid treatment, water-evoked swallowing was impaired on day 1 (prolonging the swallowing latency and interval and decreasing the number of swallows), and recovered until day 6. Administration of loxoprofen significantly alleviated impaired water swallowing. Histologically, loxoprofen significantly improved inflammation of the pharynx, including submucosal edema, but did not affect the loss of taste bud-like structures in the arytenoid. In contrast, the administration of antibacterial drugs or TRP channel inhibitors did not affect the impairment of water swallowing in the model group.

Conclusion

These results demonstrate the efficacy of systemic administration of loxoprofen in improving swallowing impairment in a pharyngitis model rat. This study is the first to provide scientific evidence for the use of NSAIDs in the management of pharyngitis.

本研究旨在探讨非甾体抗炎药（NSAIDs，如洛索洛芬）对咽炎引起的大鼠吞咽困难的影响。设计通过在喉咽部局部施用醋酸建立咽炎大鼠模型。通过内窥镜观察评估吞水量随时间的变化。对喉咽粘膜进行了组织学分析。结果经醋酸处理后，吞咽水的能力在第 1 天受到影响（吞咽潜伏期和间隔时间延长，吞咽次数减少），直到第 6 天才恢复。服用洛索洛芬可明显缓解吞水障碍。从组织学角度来看，洛索洛芬能明显改善咽部炎症，包括粘膜下水肿，但不会影响杓状体味蕾样结构的丧失。相比之下，服用抗菌药物或 TRP 通道抑制剂对模型组的吞水障碍没有影响。这项研究首次为非甾体抗炎药治疗咽炎提供了科学证据。

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引用次数: 0

Ameloblastin binding to biomimetic models of cell membranes – A continuum of intrinsic disorder 淀粉样蛋白与生物仿真细胞膜模型的结合--内在紊乱的连续体。

IF 2.2 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2024-11-01 DOI: 10.1016/j.archoralbio.2024.106124

Garry W. Buchko , Natalie C. Kegulian , Janet Moradian-Oldak

Objective

A 37-residue amino acid sequence corresponding to the segment encoded by exon-5 of murine ameloblastin (Ambn), AB2 (Y67-Q103), has been implicated with membrane association, ameloblastin self-assembly, and amelogenin-binding. Our aim was to characterize, at the residue level, the structural behavior of AB2 bound to chemical mimics of biological membranes using NMR spectroscopy.

Design

To better define the structure of AB2 using NMR-based methods, recombinant ¹³C- and ¹⁵N-labelled AB2 (*AB2) was prepared and data collected free in solution and with deuterated dodecylphosphocholine (dPC) micelles, deuterated bicelles, and both small and large unilamellar vesicles.

Results

Amide chemical shift and intensity perturbations observed in ¹H-¹⁵N HSQC spectra of *AB2 in the presence of bicelles and dPC micelles suggest that a region of *AB2, S6-E36 (murine Ambn S68 – E98), associates with the membrane biomimetics. A CSI-3 analysis of the NMR chemical shift assignments for *AB2 free in solution and bound to dPC micelles indicated the peptide remains disordered except for the adoption of a short, 12-residue α-helix, F10-G21 (murine Ambn F72-G83). In dPC micelles, the NOE NMR data was void of patterns characteristic of long-lived helical structure indicating this helix was transient in nature.

Conclusions

A continuum of intrinsic disorder in the membrane-bound state may be responsible for ameloblastin’s ability to dynamically interact with multiple partners at the same site during amelogenesis.

目的：与小鼠淀粉样蛋白（Ambn）外显子 5 所编码的片段 AB2（Y67-Q103）相对应的 37 个残基氨基酸序列与膜关联、淀粉样蛋白自组装和淀粉样蛋白结合有关。我们的目的是利用核磁共振光谱在残基水平上描述 AB2 与生物膜化学模拟物结合的结构行为：设计：为了利用基于核磁共振的方法更好地确定 AB2 的结构，我们制备了 13C 和 15N 标记的重组 AB2（*AB2），并收集了溶液中的自由数据以及与氚代十二烷基磷酸胆碱（dPC）胶束、氚代双胶束以及小型和大型单胶束囊泡结合的数据：在双胶囊和dPC胶束存在时，*AB2的1H-15N HSQC光谱中观察到的酰胺化学位移和强度扰动表明，*AB2的一个区域S6-E36（鼠Ambn S68-E98）与膜生物仿生学有关。对 *AB2 在溶液中的自由状态和与 dPC 胶束结合后的核磁共振化学位移分配进行的 CSI-3 分析表明，除了采用一个短的 12 位元 α-螺旋 F10-G21（小鼠 Ambn F72-G83）外，肽仍然是无序的。在 dPC 胶束中，NOE NMR 数据中没有长寿命螺旋结构的特征模式，这表明这种螺旋在本质上是短暂的：结论：在amelogenesis过程中，ameloblastin能够在同一部位与多个伙伴动态地相互作用，这可能是膜结合状态下内在无序性的连续性所致。

{"title":"Ameloblastin binding to biomimetic models of cell membranes – A continuum of intrinsic disorder","authors":"Garry W. Buchko , Natalie C. Kegulian , Janet Moradian-Oldak","doi":"10.1016/j.archoralbio.2024.106124","DOIUrl":"10.1016/j.archoralbio.2024.106124","url":null,"abstract":"<div><h3>Objective</h3><div>A 37-residue amino acid sequence corresponding to the segment encoded by exon-5 of murine ameloblastin (Ambn), AB2 (Y67-Q103), has been implicated with membrane association, ameloblastin self-assembly, and amelogenin-binding. Our aim was to characterize, at the residue level, the structural behavior of AB2 bound to chemical mimics of biological membranes using NMR spectroscopy.</div></div><div><h3>Design</h3><div>To better define the structure of AB2 using NMR-based methods, recombinant <sup>13</sup>C- and <sup>15</sup>N-labelled AB2 (*AB2) was prepared and data collected free in solution and with deuterated dodecylphosphocholine (dPC) micelles, deuterated bicelles, and both small and large unilamellar vesicles.</div></div><div><h3>Results</h3><div>Amide chemical shift and intensity perturbations observed in <sup>1</sup>H-<sup>15</sup>N HSQC spectra of *AB2 in the presence of bicelles and dPC micelles suggest that a region of *AB2, S6-E36 (murine Ambn S68 – E98), associates with the membrane biomimetics. A CSI-3 analysis of the NMR chemical shift assignments for *AB2 free in solution and bound to dPC micelles indicated the peptide remains disordered except for the adoption of a short, 12-residue α-helix, F10-G21 (murine Ambn F72-G83). In dPC micelles, the NOE NMR data was void of patterns characteristic of long-lived helical structure indicating this helix was transient in nature.</div></div><div><h3>Conclusions</h3><div>A continuum of intrinsic disorder in the membrane-bound state may be responsible for ameloblastin’s ability to dynamically interact with multiple partners at the same site during amelogenesis.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"169 ","pages":"Article 106124"},"PeriodicalIF":2.2,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142606756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adsorption and release pattern of recombinant human bone morphogenic proatin 2 onto different bone grafts and its consequent osteoblasts` activation and neutrophils` priming 重组人骨形态发生蛋白 2 在不同骨移植物上的吸附和释放模式及其导致的成骨细胞活化和中性粒细胞引诱。

IF 2.2 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2024-10-30 DOI: 10.1016/j.archoralbio.2024.106123

Adir Cohen , Tom Avraham Verkauf , Nardy Casap , Tali Chackartchi , David Polak

Objectives

Controlled long-term delivery of recombinant human bone morphogenic proatin 2 (rhBMP2) eluted in a collagen scaffolds suffer from only a high initial burst release. The purpose of the current study was to investigate the long-term delivery of rhBMP2 when mixed with different bone grafts and its impact on osteoblastic activity and neutrophil priming.

Methods

rhBMP2 was separately mixed with xenograft, allograft, or alloplast and incubated for 30 days. Levels of BMP2 adsorption and their release were measured using immunofluorescence and ELISA respectively. The supernatants from the grafts were then incubated with either osteoblast (Saos-2 cells) or neutrophils (differentiated from HL60) for alkaline phosphatase and oxidative stress measurements respectively. Gene expression of osteoblast functionality and neutrophil priming were measured with qRT-PCR.

Results

rhBMP2 was adsorbed onto all tested grafts, with a superior effect of alloplast. The release of the rhBMP2 from all grafts was similar and sustained for 30 days with the lowest levels in the alloplast group. Activation of osteoblast was robust in the allograft and xenograft groups, concomitant with elevated osteocalcin expression. Neutrophil priming was greatest in the xenograft group, together with elevated expression of interleukin 1β.

Conclusion

rhBMP2 with bone graft material constitutes its sustained release over time. This, in turn, robust osteoblast and neutrophil activity.

目的：在胶原支架中洗脱的重组人骨形态发生蛋白2（rhBMP2）的可控长期给药仅有较高的初始迸发释放。本研究的目的是调查 rhBMP2 与不同骨移植物混合后的长期输送及其对成骨细胞活性和中性粒细胞引物的影响。分别用免疫荧光法和酶联免疫吸附法测定 BMP2 的吸附水平和释放水平。然后将移植物的上清液与成骨细胞（Saos-2 细胞）或中性粒细胞（从 HL60 分化而来）培养，分别进行碱性磷酸酶和氧化应激测定。结果：rhBMP2 被吸附在所有测试的移植物上，其中异体移植物的效果更好。所有移植物的 rhBMP2 释放量相似，并可持续 30 天，其中 alloplast 组的释放量最低。异体移植物组和异种移植物组的成骨细胞活化很强，同时骨钙素的表达也升高。结论：rhBMP2 与骨移植材料一起使用可使其长期持续释放。结论：rhBMP2 与骨移植材料一起使用可使其持续释放，进而增强成骨细胞和中性粒细胞的活性。

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引用次数: 0

Porphyromonas gingivalis-induced autophagy exacerbates abnormal lung homeostasis: An in vivo and in vitro study 牙龈卟啉单胞菌诱导的自噬作用加剧了肺稳态异常：体内和体外研究

IF 2.2 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2024-10-29 DOI: 10.1016/j.archoralbio.2024.106122

Qian Zhao , Wenyue Li , Wei Li, Hongjia Yang, Xueyuan Wang, Zhaoyue Ding, Zhiqiang Liu, Zuomin Wang

Objective

The aim of this study was to evaluate the effect of periodontal Porphyromonas gingivalis (P. gingivalis) infection on lung homeostasis and to explore the underlying mechanism.

Designs

In in vivo experiments, twelve mice were divided into two groups. The P. gingivalis infection group received P. gingivalis around the maxillary second molar, and the control group was left untreated. After 12 weeks, the histopathological changes of the lung tissue and the autophagy and apoptosis in the lung tissue cells were detected. In in vitro experiments, alveolar epithelial cell A549 was co cultured with P. gingivalis and treated with autophagy inhibitor chloroquine (CQ). Western blot was then used to detect autophagic markers LC3 and P62, and mRFP-GFP-LC3 was used to observe autophagic flux. Cell viability and apoptosis were also detected.

Results

For the in vivo experiments, pathological changes were observed in the lung tissue of the P. gingivalis infection group at 12 weeks, along with higher levels of autophagy and apoptosis in the lung tissue cells. For the in vitro experiments, infection of alveolar epithelial cells with P. gingivalis inhibited cell viability and promoted cell autophagy and apoptosis. Interestingly, we found that inhibiting P. gingivalis-activated autophagy significantly improved cell apoptosis and viability damage induced by P. gingivalis.

Conclusion

Periodontal P. gingivalis infection can cause pathological changes and abnormal homeostasis in lung tissue, and the up-regulation of autophagy induced by P. gingivalis may play a synergistic role in this process.

设计在活体实验中，将 12 只小鼠分为两组。牙龈弧菌感染组在上颌第二磨牙周围接受牙龈弧菌感染，对照组不做任何处理。12 周后，检测肺组织的组织病理学变化以及肺组织细胞的自噬和凋亡。在体外实验中，将肺泡上皮细胞 A549 与牙龈脓疱病菌共同培养，并用自噬抑制剂氯喹（CQ）处理。然后用 Western 印迹检测自噬标记物 LC3 和 P62，并用 mRFP-GFP-LC3 观察自噬通量。结果在体内实验中，牙龈脓疱病感染组的肺组织在 12 周后出现病理变化，肺组织细胞的自噬和凋亡水平较高。在体外实验中，牙龈弧菌感染肺泡上皮细胞会抑制细胞活力，促进细胞自噬和凋亡。有趣的是，我们发现抑制牙龈脓疱噬菌体激活的自噬作用可明显改善牙龈脓疱噬菌体诱导的细胞凋亡和活力损伤。

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引用次数: 0