Archives of oral biology最新文献_第4页

Insulin-like growth factor-binding protein 3 regulates collagen production via TGF-β-Smad2/3 pathway and osteogenic differentiation related with phosphorylation of Akt in human periodontal ligament-derived cell line 胰岛素样生长因子结合蛋白3通过TGF-β-Smad2/3通路调节人牙周韧带源性细胞系胶原蛋白的生成和Akt磷酸化相关的成骨分化

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2026-03-01 Epub Date: 2025-12-17 DOI: 10.1016/j.archoralbio.2025.106483

Shuxin Wang , Hiromi Mitarai , Hiroko Wada , Ziqing Ran , Asuka Yuda , Akira Haraguchi , Weihao Sun , Jiawen Lin , Hidefumi Maeda , Naohisa Wada

Objective

Insulin-like growth factor-binding protein 3 (IGFBP3) is a multifunctional protein involved in various cellular functions. However, the function of IGFBP3 in periodontal ligament (PDL) tissue remains unclear. In this study, we investigated the localization and function of IGFBP3 in PDL tissues and human PDL-derived cell line.

Design

Small interfering RNA (siRNA) and recombinant protein of IGFBP3 were used to examine the effect of IGFBP3 in human PDL-derived cell line. mRNA expression was determined by quantitative reverse transcription-polymerase chain reaction. Protein expression was determined using immunohistochemical staining, immunofluorescence staining, and Western blotting analyses. WST-1 and migration assays were used to analyze the effects on proliferation and migration. Collagen production was examined using picrosirius red staining. Calcium nodule formation was examined using Alizarin Red S staining.

Results

IGFBP3 was expressed in both mouse PDL tissues and human PDL-derived cell line (2–23 cells). Mechanical stretch and Transforming growth factor-beta 1 (TGF-β1) stimulation upregulated IGFBP3 expression in 2–23 cells. Knockdown of IGFBP3 using siRNA significantly suppressed PDL-related gene expression, collagen production, and inhibited Smad2/3 phosphorylation induced by TGF-β1, while IGFBP3 knockdown enhanced calcium chloride-induced osteogenic differentiation in 2–23 cells and activated Akt phosphorylation. Furthermore, treatment with exogenous rhIGFBP3 showed the opposite trend.

Conclusions

IGFBP3 plays a dual role in PDL homeostasis, by promoting collagen production and inhibiting osteogenic differentiation. IGFBP3 is involved in TGF-β-Smad2/3 pathway and related with phosphorylation of Akt, highlighting IGFBP3 as a potential therapeutic target for periodontal regeneration.

目的胰岛素样生长因子结合蛋白3 （IGFBP3）是一种参与多种细胞功能的多功能蛋白。然而，IGFBP3在牙周韧带（PDL）组织中的功能尚不清楚。在本研究中，我们研究了IGFBP3在PDL组织和人PDL来源细胞系中的定位和功能。设计采用小干扰RNA （siRNA）和IGFBP3重组蛋白检测IGFBP3在人pdl源性细胞系中的作用。定量逆转录-聚合酶链反应测定mRNA表达。采用免疫组织化学染色、免疫荧光染色和Western blotting分析测定蛋白表达。采用WST-1和迁移试验分析对增殖和迁移的影响。小天狼星红染色检测胶原蛋白的生成。茜素红S染色检测钙结节形成。结果igfbp3在小鼠PDL组织和人PDL来源细胞系（2-23细胞）中均有表达。机械拉伸和转化生长因子-β1 （TGF-β1）刺激上调2-23细胞IGFBP3表达。用siRNA敲低IGFBP3可显著抑制pdl相关基因表达、胶原生成，抑制TGF-β1诱导的Smad2/3磷酸化，而敲低IGFBP3可增强氯化钙诱导的2-23细胞成骨分化，激活Akt磷酸化。此外，外源性rhIGFBP3处理表现出相反的趋势。结论sigfbp3具有促进胶原生成和抑制成骨分化的双重作用。IGFBP3参与TGF-β-Smad2/3通路，并与Akt磷酸化有关，因此IGFBP3是牙周再生的潜在治疗靶点。

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引用次数: 0

Rutin (Vitamin P) attenuates oxidative stress, modulates cytokine profile, and preserves alveolar bone microarchitecture and density in a rat periodontitis model 芦丁（维生素P）在大鼠牙周炎模型中减轻氧化应激，调节细胞因子分布，并保持牙槽骨微结构和密度

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2026-03-01 Epub Date: 2025-12-16 DOI: 10.1016/j.archoralbio.2025.106485

Sami Barış Keskin , Muhammed Mehdi Üremiş , Yusuf Türköz , Cüneyt Asım Aral

Objectives

To evaluate the anti-inflammatory and antioxidant effects of rutin in experimental periodontitis.

Design

Wistar Albino rats were divided into four groups (n = 8 per group): 1) Healthy Control (HC), 2) Periodontitis (P), 3) P + Rutin 50 mg/kg (PR-50), and 4) P + Rutin 100 mg/kg (PR-100). Rutin was administered orally throughout the 14-day experimental period. Gingival levels of interleukin-1 beta (IL-1β), interleukin-10 (IL-10), glutathione peroxidase (GPX), malondialdehyde (MDA), and superoxide dismutase (SOD) and were measured using enzyme-linked immunosorbent assay, while oxidative stress index (OSI), total oxidant (TOS), and antioxidant status (TAS) were analysed spectrophotometrically. Alveolar bone was assessed by micro-computed tomography, including linear (ABL), volumetric (BV/TV), microarchitectural (BS/BV, Tb.Th, Tb.N, Tb.Sp), and densitometric (GV, HU, BMD) parameters.

Results

IL-1β/IL-10 ratio was lower, and IL-10 and GPX levels were higher in PR-100 group than in P group (p < 0.05). SOD levels were higher in HC and PR-100 groups than in P group (p < 0.001). OSI was highest in P (p < 0.001). TOS was higher in P versus HC, whereas TAS was lower in PR-50 versus HC (p < 0.05). ABL and BV/TV analyses showed significant bone preservation in both rutin groups compared to P (p < 0.01). Microstructural and densitometric indices further confirmed less alveolar bone deterioration in rutin-treated rats.

Conclusion

Rutin exerts dose-dependent anti-inflammatory, antioxidant, and bone-protective effects in experimental periodontitis, suggesting its potential as an adjunctive therapeutic agent.

目的探讨芦丁对实验性牙周炎的抗炎、抗氧化作用。将DesignWistar Albino大鼠分为4组（每组 = 8只）：1)健康对照组（HC）， 2)牙周炎组（P), 3) P + 芦丁50 mg/kg (PR-50), 4） P + 芦丁100 mg/kg （PR-100）。在14 d的试验期内口服芦丁。采用酶联免疫吸附法测定牙龈白细胞介素-1β (IL-1β)、白细胞介素-10 （IL-10）、谷胱甘肽过氧化物酶（GPX）、丙二醛（MDA）、超氧化物歧化酶（SOD）水平，分光光度法分析氧化应激指数（OSI）、总氧化剂（TOS）、抗氧化状态（TAS）。通过显微计算机断层扫描评估牙槽骨，包括线性（ABL），体积（BV/TV），微结构（BS/BV, Tb）。Th,结核病。N,结核病。Sp)和密度测量（GV、HU、BMD）参数。结果PR-100组il -1β/IL-10比值低于P组，IL-10和GPX水平高于P组（P <； 0.05）。HC和PR-100组SOD水平高于P组（P <； 0.001）。OSI以P最高（P <； 0.001）。TOS在P组高于HC组，TAS在PR-50组低于HC组（P <； 0.05）。ABL和BV/TV分析显示，与P相比，两组芦丁均有显著的骨保护作用（P <； 0.01）。显微结构和密度指数进一步证实，芦丁治疗大鼠的牙槽骨退化程度较低。结论芦丁对实验性牙周炎具有剂量依赖性的抗炎、抗氧化和骨保护作用，具有作为辅助治疗药物的潜力。

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引用次数: 0

Evaluation of the effects induced by radiotherapy on dentin during endodontic instrumentation using a novel methodology for assessing wear resistance 使用一种评估耐磨性的新方法评估放射治疗对牙本质的影响。

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2026-03-01 Epub Date: 2026-01-11 DOI: 10.1016/j.archoralbio.2025.106493

Leonardo Moreira Teodosio , Ana Flavia Simões Barbosa , Alexandra Mussolino de Queiroz , Harley Francisco de Oliveira , Camila Andrade Zamperini , Jardel Francisco Mazzi-Chaves , Manoel D. Sousa-Neto , Yara Teresinha Corrêa Silva-Sousa , Fuad Jacob Abi Rached-Junior , Edson Alfredo , Fabiane Carneiro Lopes-Olhê

Objective

This study evaluated a novel methodology for assessing dentin wear resistance and morphological changes in teeth submitted to conventional (CR) or hypofractionated radiotherapy (HR).

Design

Fifteen maxillary canines were divided into three groups: control, CR (60 Gy, 2 Gy/day) and HR (55 Gy, 2.75 Gy/day). The roots were sectioned 3 mm from the apex and 3 mm above and fixed in acrylic resin and tested on a universal testing machine by measuring the force required to wear away the dentin. The specimens were submitted to microhardness analysis with indentations at 50, 100 and 150 µm from the radicular canal surface, scanning electron microscopy (SEM) to identify cracks and energy dispersive X-ray spectroscopy (EDS-X) to quantify calcium (Ca) and phosphorus (P) and analyze the Ca/P ratio.

Results

No significant difference in wear resistance (MPa) was detected between the irradiated groups (CR: 0.102 ± 0.01, HR: 0.111 ± 0.01) and the control group (0.114 ± 0.02) (p > 0.05). Lower microhardness values were observed in the irradiated groups (CR: 37.92 ± 5.78, HR: 32.71 ± 4.37) compared to the control (50.00 ± 3.31), with no difference between them (p > 0.05). The irradiated groups had a higher number of cracks compared to the control, and there was no statistically significant difference in the Ca/P ratio values between the groups tested (p > 0.05).

Conclusions

The new method assessed the wear resistance of dentin after radiotherapy reliably. Although wear resistance and the Ca/P ratio were not altered, both radiotherapy protocols reduced dentin microhardness.

目的：研究一种评估常规（CR）或低分割放射治疗（HR）牙本质耐磨性和形态变化的新方法。设计：15只上颌犬分为3组：对照组、CR组（60 Gy, 2 Gy/day）和HR组（55 Gy, 2.75 Gy/day）。根从顶点处3 mm和3 mm处切割，固定在丙烯酸树脂中，并通过测量磨损牙本质所需的力在通用试验机上进行测试。样品在距离根管表面50、100和150 µm处进行显微硬度分析，扫描电镜（SEM）鉴定裂纹，能量色散x射线光谱（EDS-X）定量钙（Ca）和磷(P)并分析Ca/P比值。结果：辐照组（CR: 0.102 ± 0.01,HR: 0.111 ± 0.01）与对照组（0.114 ± 0.02）（p > 0.05）的耐磨性（MPa）无显著差异。辐照组显微硬度值（CR: 37.92 ± 5.78,HR: 32.71 ± 4.37）低于对照组（50.00 ± 3.31），两组间无差异（p > 0.05）。与对照组相比，辐照组的裂缝数量较多，Ca/P比值在各组之间无统计学差异（P > 0.05）。结论：新方法能可靠地评价放射治疗后牙本质的耐磨性。虽然耐磨性和Ca/P比值没有改变，但两种放疗方案都降低了牙本质显微硬度。

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引用次数: 0

HOXD10 expression in oral cancer is driven by histone acetylation rather than promoter DNA methylation HOXD10在口腔癌中的表达是由组蛋白乙酰化而不是启动子DNA甲基化驱动的

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2026-03-01 Epub Date: 2026-01-08 DOI: 10.1016/j.archoralbio.2026.106512

Dhanraj Salur Basavarajappa , U Sangeetha Shenoy , Naveena Kumar AN , Shama Prasada Kabekkodu , Sanjiban Chakrabarty , Raghu Radhakrishnan

Objective

HOXD10 is implicated in the carcinogenesis and progression of various cancers, including oral cancer. However, its regulatory mechanism and functional role remain unclear.

Design

Matched normal and oral cancer tissue samples from patients stratified into oral potentially malignant disorders (OPMD, n = 25), lymph-node negative (LN(-), n = 25), and lymph-node positive (LN(+), n = 25) groups were analysed along with a panel of oral cancer cell lines. Expression was quantified by qRT-PCR and DNA methylation was profiled by methyl-capture sequencing followed by correlation analysis with expression. The DNA methyltransferase inhibitor(DNMTi): Decitabine(5-Aza-CdR) and histone deacetylase inhibitors(HDACi) – suberoylanilide hydroxamic acid(SAHA) and sodium butyrate(NaB) were used to evaluate epigenetic regulation in SCC9 cells. Promoter regions were characterized by dual luciferase assay and sodium butyrate effects on cell proliferation, migration and cell cycle were assessed.

Results

HOXD10 was significantly upregulated in OPMD and LN(+) groups and oral cancer cell lines but not in the LN(-) samples or across cancer stages/grades compared with normal controls. Promoter hypermethylation correlated positively, though not significantly, with expression. 5-Aza-CdR treatment did not alter HOXD10 levels, while HDACi treatment reduced expression. Two promoter regions were identified as active regulatory elements modulated by histone acetylation. Functionally, NaB impaired cell proliferation, migration and induced G2-M arrest. Survival analysis demonstrated modest prognostic value of HOXD10 alone but improved predictive accuracy for disease recurrence when integrated with tumor stage and grade.

Conclusion

HOXD10 expression in oral cancer is regulated predominantly via histone acetylation. These findings highlight its epigenetic regulation and suggest potential clinical relevance with tumor stage and grade although further studies are needed to confirm diagnostic or prognostic value.

目的：hoxd10参与多种癌症的发生和发展，包括口腔癌。但其调控机制和功能作用尚不清楚。设计将匹配的正常和口腔癌组织样本分为口腔潜在恶性疾病（OPMD, n = 25）、淋巴结阴性（LN(-), n = 25）和淋巴结阳性（LN(+), n = 25）组，并对一组口腔癌细胞系进行分析。通过qRT-PCR定量表达，通过甲基捕获测序分析DNA甲基化，然后进行与表达的相关性分析。采用DNA甲基转移酶抑制剂（DNMTi）：地西他滨（5-Aza-CdR）和组蛋白去乙酰化酶抑制剂(HDACi) -亚eroylanilide羟肟酸（SAHA）和丁酸钠（NaB）评估SCC9细胞的表观遗传调控。用双荧光素酶法对启动子区域进行了表征，并评估了丁酸钠对细胞增殖、迁移和细胞周期的影响。结果与正常对照相比，OPMD组、LN（+）组和口腔癌细胞系中shoxd10表达显著上调，而LN（-）组和不同癌症分期/分级中shoxd10表达均不上调。启动子超甲基化与表达呈正相关，但不显著。5-Aza-CdR治疗没有改变HOXD10水平，而HDACi治疗降低了HOXD10的表达。两个启动子区域被确定为由组蛋白乙酰化调节的活性调控元件。功能上，NaB损害细胞增殖、迁移和诱导G2-M阻滞。生存分析显示HOXD10单独的预后价值不大，但当结合肿瘤分期和分级时，疾病复发的预测准确性提高。结论hoxd10在口腔癌组织中的表达主要受组蛋白乙酰化调控。这些发现强调了其表观遗传调控，并提示与肿瘤分期和分级的潜在临床相关性，尽管需要进一步的研究来确认诊断或预后价值。

{"title":"HOXD10 expression in oral cancer is driven by histone acetylation rather than promoter DNA methylation","authors":"Dhanraj Salur Basavarajappa , U Sangeetha Shenoy , Naveena Kumar AN , Shama Prasada Kabekkodu , Sanjiban Chakrabarty , Raghu Radhakrishnan","doi":"10.1016/j.archoralbio.2026.106512","DOIUrl":"10.1016/j.archoralbio.2026.106512","url":null,"abstract":"<div><h3>Objective</h3><div><em>HOXD10</em> is implicated in the carcinogenesis and progression of various cancers, including oral cancer. However, its regulatory mechanism and functional role remain unclear.</div></div><div><h3>Design</h3><div>Matched normal and oral cancer tissue samples from patients stratified into oral potentially malignant disorders (OPMD, n = 25), lymph-node negative (LN(-), n = 25), and lymph-node positive (LN(+), n = 25) groups were analysed along with a panel of oral cancer cell lines. Expression was quantified by qRT-PCR and DNA methylation was profiled by methyl-capture sequencing followed by correlation analysis with expression. The DNA methyltransferase inhibitor(DNMTi): Decitabine(5-Aza-CdR) and histone deacetylase inhibitors(HDACi) – suberoylanilide hydroxamic acid(SAHA) and sodium butyrate(NaB) were used to evaluate epigenetic regulation in SCC9 cells. Promoter regions were characterized by dual luciferase assay and sodium butyrate effects on cell proliferation, migration and cell cycle were assessed.</div></div><div><h3>Results</h3><div><em>HOXD10</em> was significantly upregulated in OPMD and LN(+) groups and oral cancer cell lines but not in the LN(-) samples or across cancer stages/grades compared with normal controls. Promoter hypermethylation correlated positively, though not significantly, with expression. 5-Aza-CdR treatment did not alter <em>HOXD10</em> levels, while HDACi treatment reduced expression. Two promoter regions were identified as active regulatory elements modulated by histone acetylation. Functionally, NaB impaired cell proliferation, migration and induced G2-M arrest. Survival analysis demonstrated modest prognostic value of <em>HOXD10</em> alone but improved predictive accuracy for disease recurrence when integrated with tumor stage and grade.</div></div><div><h3>Conclusion</h3><div><em>HOXD10</em> expression in oral cancer is regulated predominantly via histone acetylation. These findings highlight its epigenetic regulation and suggest potential clinical relevance with tumor stage and grade although further studies are needed to confirm diagnostic or prognostic value.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"183 ","pages":"Article 106512"},"PeriodicalIF":2.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145973208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Topical sevoflurane enhances periodontal wound healing in a male rat palatal mucosal model 局部七氟醚促进雄性大鼠腭粘膜模型牙周伤口愈合。

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2026-03-01 Epub Date: 2025-12-25 DOI: 10.1016/j.archoralbio.2025.106491

Gülenay Çolak , Zeynep Turgut Çankaya , Çiğdem Elmas , Gökçe Nur Arık Erol

Objective

The aim of this study is to evaluate the effect of topical sevoflurane, which has recently been reported to possess analgesic, anti-inflammatory and antibacterial properties, on secondary wound healing in rat palatal mucosa.

Design

Thirty male Wistar Albino rats were randomly divided into three groups: Control, Saline (0.9 % NaCl) and Sevoflurane. Standardized full-thickness excisional wounds (4 mm) were created on the hard palate, and treatment was applied once daily for 14 days. Wound closure, histopathological healing and immunohistochemical expression of vascular endothelial growth factor, interleukin-17A, interleukin-22 and signal transducer and activator of transcription 3 were evaluated on days 5 and 14.

Results

On day 5, the sevoflurane group exhibited smaller wound areas (p < 0.05) and greater angiogenesis, epithelialization, and granulation than the control group. On day 14, epithelial integrity and vascular remodeling were most pronounced in the sevoflurane group. IL-22 and pSTAT3 expression levels were higher, while IL-17A expression was lower (p < 0.05). VEGF expression decreased over time, indicating progression toward tissue remodeling.

Conclusions

Topical sevoflurane enhances early-stage wound healing by modulating inflammatory and regenerative pathways, promoting angiogenesis, and reducing wound size. These findings suggest that sevoflurane may serve as a novel adjunct for periodontal wound healing.

目的：本研究的目的是评价外用七氟醚对大鼠腭黏膜继发性伤口愈合的影响，七氟醚最近被报道具有镇痛、抗炎和抗菌的特性。设计：30只雄性Wistar Albino大鼠随机分为3组：对照组、生理盐水（0.9 % NaCl）组和七氟醚组。在硬腭建立标准化全层切除创面（4 mm），每日1次，连用14天。第5、14天观察创面闭合、组织病理愈合及血管内皮生长因子、白细胞介素- 17a、白细胞介素-22、信号转导因子和转录激活因子3的免疫组化表达。结果：第5天，七氟醚组创面面积较小（p ）结论：局部七氟醚通过调节炎症和再生途径、促进血管生成和减少创面大小来促进早期创面愈合。这些发现表明，七氟醚可能作为一种新的辅助牙周伤口愈合。

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引用次数: 0

Investigating the mechanism of berberine in inhibiting periodontitis progression using machine learning, molecular docking, and experimental validation 利用机器学习、分子对接和实验验证研究小檗碱抑制牙周炎进展的机制

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2026-03-01 Epub Date: 2026-01-06 DOI: 10.1016/j.archoralbio.2025.106496

Zhonghua Wang , Zhenli Liu , Sai Ma , Xiaohui Jia , Xu Zhang , Zhiyi Zhang , Chao Li , Jinping Xiao , Yan Si

Background

Periodontitis is a chronic inflammatory disease characterized by the destruction of periodontal tissues. Berberine (BBR), a natural alkaloid with anti-inflammatory and antioxidant properties, has shown potential in treating inflammatory diseases.

Methods

Differentially expressed genes (DEGs) between periodontitis and healthy gingival tissues were identified using the GSE23586 and GSE173078 datasets, while chronic periodontitis-related genes were retrieved from the GeneCards database. Key genes were screened through support vector machine (SVM) and random forest (RF) algorithms, molecular docking, and normal mode analysis. In vitro, human gingival fibroblast-1 (HGF-1) cells were treated with lipopolysaccharide (LPS) to simulate periodontitis.

Results

Six key genes (KRT14, IGFBP6, LRG1, MZB1, DEFB103A, and CD79A) were identified by both SVM and RF algorithms. LRG1 was selected for further study due to its significant downregulation after BBR treatment in LPS-treated HGF-1 cells. LPS treatment increased LRG1 expression and the p-p38/p38 ratio, whereas these effects reversed by BBR treatment. Overexpression of LRG1 diminished BBR’s inhibitory effect on p-p38/p38, while the p38 MAPK pathway inhibitor restored BBR’s efficacy. BBR treatment suppressed LPS-induced inflammatory response, oxidative stress, and ECM degradation, but these effects were relieved by ectopic LRG1 expression.

Conclusion

BBR alleviated LPS-induced periodontitis by inhibiting inflammation, oxidative stress, and ECM degradation through inactivation of the LRG1/p38 MAPK signaling pathway. These findings highlight BBR’s potential as a therapeutic agent for periodontitis, offering a novel molecular target for clinical intervention. Further in vivo studies are warranted to validate its clinical application.

牙周炎是一种以牙周组织破坏为特征的慢性炎症性疾病。小檗碱（BBR）是一种具有抗炎和抗氧化特性的天然生物碱，在治疗炎症性疾病方面已显示出潜力。方法使用GSE23586和GSE173078数据集鉴定牙周炎与健康牙龈组织之间的差异表达基因（DEGs），并从GeneCards数据库检索慢性牙周炎相关基因。通过支持向量机（SVM）和随机森林（RF）算法、分子对接和正态分析筛选关键基因。在体外，用脂多糖（LPS）处理人牙龈成纤维细胞-1 （HGF-1）细胞，模拟牙周炎。结果通过SVM和RF算法分别鉴定出KRT14、IGFBP6、LRG1、MZB1、DEFB103A和CD79A 6个关键基因。LRG1在lps处理的HGF-1细胞中经过BBR处理后显著下调，因此我们选择LRG1作为进一步研究的对象。LPS处理增加了LRG1表达和p-p38/p38比值，而BBR处理逆转了这些作用。过表达LRG1可减弱BBR对p-p38/p38的抑制作用，而p38 MAPK通路抑制剂可恢复BBR的抑制作用。BBR治疗抑制lps诱导的炎症反应、氧化应激和ECM降解，但这些作用通过异位LRG1表达而缓解。结论bbr通过抑制LRG1/p38 MAPK信号通路的失活，抑制炎症、氧化应激和ECM降解，从而减轻lps诱导的牙周炎。这些发现突出了BBR作为牙周炎治疗剂的潜力，为临床干预提供了新的分子靶点。需要进一步的体内研究来验证其临床应用。

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引用次数: 0

Prevalence and eradication of mixed biofilms of Streptococcus mutans and Candida albicans using naringin: In vitro and in silico investigations 柚皮苷对变形链球菌和白色念珠菌混合生物膜的流行和根除：体外和计算机研究

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2026-03-01 Epub Date: 2025-12-13 DOI: 10.1016/j.archoralbio.2025.106482

Chevuru Sai Shreya Reddy , Lekha Sree Venkatesan , Dhanraj Ganapathy, Palanivel Sathishkumar

Objective

To identify a potential natural therapeutic agent to treat the mixed biofilms of Streptococcus mutans and Candida albicans, thereby preventing dental caries.

Design

The coexisting S. mutans and C. albicans was isolated from dental caries. Antimicrobial activity of various natural components was assessed against S. mutans and C. albicans. Antibiofilm efficacy of naringin was assessed against the biofilm of mixed S. mutans and C. albicans. The cell viability in mixed biofilm was determined using MTT assay and CLSM investigations. The molecular docking analysis of naringin with secreted aspartic proteinase (SAP2) of C. albicans and glucosyltransferase-I (GtfB) of S. mutans was performed using AutoDock and PyMOL tools. The biocompatibility of naringin was determined on human RBCs and HGF cells.

Results

The natural components such as curcumin, naringin, quercetin, morin, rutin, and hesperetin shows the antimicrobial effects against the clinical isolates S. mutans and C. albicans. Among these, naringin exhibits the significant antimicrobial effect against S. mutans (MIC:183 ± 5.70 µM), C. albicans (MIC:196 ± 0.00 µM), and their mixed culture (MIC:200 ± 10.00 µM). The MTT and CLSM results indicates that 2xMIC (400 µM) of naringin demonstrates the potential eradication of mature biofilm of mixed S. mutans and C. albicans. Naringin establishes the strong binding interaction with enzymes GtfB of S. mutans and SAP2 of C. albicans. The IC₅₀ value of naringin towards HGF cells was noted as 711.5 µM.

Conclusions

The flavonoid naringin demonstrates the effective and safe therapeutic potential to treat coexisting S. mutans and C. albicans biofilm virulence to prevent the dental caries.

目的寻找一种治疗变形链球菌和白色念珠菌混合生物膜的潜在天然治疗剂，以预防龋病的发生。设计从龋中分离出变形链球菌和白色念珠菌。评估了各种天然成分对变形链球菌和白色念珠菌的抑菌活性。研究了柚皮苷对变形链球菌和白色念珠菌混合生物膜的抗菌效果。采用MTT法和CLSM法测定混合生物膜中的细胞活力。利用AutoDock和PyMOL工具对柚皮苷与白念珠菌分泌的天冬氨酸蛋白酶（SAP2）和变形链球菌的葡萄糖基转移酶（GtfB）进行分子对接分析。测定柚皮苷在人红细胞和HGF细胞上的生物相容性。结果姜黄素、柚皮素、槲皮素、桑皮素、芦丁、橙皮素等天然成分对临床分离的变形链球菌和白色念珠菌均有抑菌作用。其中,柚皮苷表现出显著的抗菌效应对变形链球菌(麦克风:183 ±5.70  µM),白念珠菌(麦克风:196 ±0.00  µM),及其混合文化(麦克风:200 ±10.00  µM)。MTT和CLSM结果表明，柚皮苷的2xMIC（400 µM）显示出对混合变形链球菌和白色念珠菌成熟生物膜的潜在根除作用。柚皮苷与变形链球菌的GtfB酶和白色念珠菌的SAP2酶有较强的结合作用。柚皮素对HGF细胞的IC50值为711.5 µM。结论黄酮类柚皮苷对变形链球菌和白色念珠菌共存的生物膜毒力具有安全有效的治疗作用。

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引用次数: 0

Antimicrobial effect of garlic-based mouthwash on dental caries-related oral microorganisms: A systematic review and meta-analysis 大蒜漱口水对龋齿相关口腔微生物的抗菌作用：系统综述和荟萃分析。

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2026-03-01 Epub Date: 2025-12-31 DOI: 10.1016/j.archoralbio.2025.106495

Renata de Oliveira Alves , José Roberto Vergínio de Matos , Matheus Henrique Faccioli Ragghianti , Isabela Maria Passarela Gomes , Isabela dos Santos de Deus , Pamela Barbosa dos Santos , Marcelle Danelon , Gabriel Pereira Nunes

Objective

This systematic review and meta-analysis (SRM) investigated the potential of garlic (Allium sativum) against cariogenic oral microorganisms.

Design

The SRM was conducted following PRISMA guidelines and registered in PROSPERO (CRD420251133140). Randomized clinical trials (RCTs) evaluating garlic or garlic-derived compounds on cariogenic oral microorganisms were included. A literature search was performed in the main scientific databases. Meta-analyses were performed using RevMan software, with standardized mean difference (SMD) as the effect measure, and a random-effects model was applied with 95 % confidence intervals. Risk of bias was assessed using the Cochrane tool, and the certainty of evidence was graded according to the GRADE approach.

Results

Nine RCTs fulfilled the inclusion criteria, predominantly assessing S. mutans, followed by Lactobacillus spp. and C. albicans. All included trials employed garlic-based mouthwashes as the intervention and consistently demonstrated significant antimicrobial activity. In meta-analysis, compared to chlorhexidine, garlic reduced S. mutans at 1 week (SMD = −0.73, 95 % CI = −1.39 to −0.07, I² = 73 %; p = 0.03), had a slightly lower effect at 2 weeks (SMD = 1.27, 95 % CI = 0.09–2.44, I² = 90 %; p = 0.03), and showed no difference at 1 month (SMD = −0.54, 95 % CI = −2.78–1.70, I² = 96 %; p = 0.64). Compared to sodium fluoride, it demonstrated superior activity at 2 weeks (SMD = −0.79, 95 % CI = −1.22 to −0.36, I² = 0 %; p = 0.0003). Most studies had a low risk of bias, and the certainty of the evidence was rated low.

Conclusions

Garlic-based mouthrinses show significant antimicrobial activity against cariogenic microorganisms, supporting their potential as a phytotherapeutic strategy for biofilm control. However, the evidence remains limited, demonstrating the need for further high-quality clinical trials to confirm long-term efficacy.

目的：本系统综述和荟萃分析（SRM）探讨大蒜（Allium sativum）对口腔致龋微生物的潜在作用。设计：SRM按照PRISMA指南进行，并在PROSPERO注册（CRD420251133140）。随机临床试验（rct）评估大蒜或大蒜衍生化合物对龋病口腔微生物。在主要科学数据库中进行文献检索。采用RevMan软件进行meta分析，采用标准化平均差（SMD）作为效应测度，采用随机效应模型，置信区间为95% %。使用Cochrane工具评估偏倚风险，并根据GRADE方法对证据的确定性进行分级。结果：9项随机对照试验符合纳入标准，主要评估变形链球菌，其次是乳酸杆菌和白色念珠菌。所有纳入的试验均采用大蒜漱口水作为干预措施，并始终显示出显著的抗菌活性。在荟萃分析,洗必泰相比,大蒜降低变形链球菌在1周(SMD = -0.73, 95 % CI = -1.39 - -0.07,我²= 73 %;p = 0.03),略低的影响在两周(SMD = 1.27, 95 % CI = 0.09 - -2.44,我²= 90 %;p = 0.03),并显示在1个月没有区别(SMD = -0.54, 95 % CI = -2.78 - -1.70,我²= 96 %;p = 0.64)。与氟化钠相比，它在2周时表现出更好的活性（SMD = -0.79, 95 % CI = -1.22至-0.36,I²= 0 %;p = 0.0003）。大多数研究的偏倚风险较低，证据的确定性评级较低。结论：大蒜漱口水对致龋微生物具有显著的抗菌活性，支持其作为生物膜控制的植物治疗策略的潜力。然而，证据仍然有限，表明需要进一步的高质量临床试验来确认长期疗效。

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引用次数: 0

The exposome and the human oral microbiome through the one health lens 暴露体和人类口腔微生物组通过一个健康镜头。

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2026-03-01 Epub Date: 2026-01-08 DOI: 10.1016/j.archoralbio.2026.106504

Luis Limo , Justin Donovan , Stephanie Frisbee , Noha Gomaa

Objectives

To map and synthesise current evidence on how lifelong biological, environmental, and social exposures, collectively conceptualised as the exposome, interact with the human oral microbiome to influence oral disease development and progression within a One Health framework.

Design

A scoping review was conducted to identify peer-reviewed studies published in English that examined the relationships between the exposome and the human oral microbiome. The review followed the Arksey and O’Malley framework, applying its five-stage methodological approach. Comprehensive searches were performed in MEDLINE, Embase, PsycInfo, Scopus, Web of Science, and CINAHL. Study quality was assessed using the Joanna Briggs Institute (JBI) tools, and results were reported in accordance with PRISMA-ScR guidelines.

Results

A total of twenty-nine studies were included in this review. These showed that health status, non-communicable diseases, medication use, and psychosocial factors influence the biodiversity, abundance, and function of the human oral microbiome. Other studies suggested that animal interactions and physical and chemical exposures can alter host-microbiome interactions, as well as microbial community dynamics within the oral cavity. While the studies reviewed used reliable methods and standardized protocols, they were of moderate quality due to small sample sizes, potential reverse causality, and limited control for confounding and multiple testing.

Conclusion

This review highlights the complexity of the human oral microbiome and its interactions with the various components of the exposome, emphasizing the focus on disease impact and health behaviours, while noting a gap in research on non-bacterial communities, interaction mechanisms, and long-term effects on dysbiosis.

目标：绘制和综合目前的证据，说明终身生物、环境和社会暴露（统称为暴露者）如何与人类口腔微生物群相互作用，从而影响“同一个健康”框架下口腔疾病的发展和进展。设计：进行了一项范围审查，以确定发表的同行评议的英文研究，这些研究检查了暴露体与人类口腔微生物群之间的关系。该评估遵循Arksey和O'Malley框架，采用其五阶段方法方法。在MEDLINE、Embase、PsycInfo、Scopus、Web of Science和CINAHL中进行综合检索。使用Joanna Briggs Institute （JBI）工具评估研究质量，并根据PRISMA-ScR指南报告结果。结果：本综述共纳入29项研究。这些研究表明，健康状况、非传染性疾病、药物使用和社会心理因素会影响人类口腔微生物群的生物多样性、丰度和功能。其他研究表明，动物相互作用和物理和化学暴露可以改变宿主-微生物组相互作用，以及口腔内微生物群落动态。虽然回顾的研究使用了可靠的方法和标准化的方案，但由于样本量小，潜在的反向因果关系，以及对混杂和多重测试的控制有限，它们的质量一般。结论：本综述强调了人类口腔微生物群的复杂性及其与暴露体各组成部分的相互作用，强调了疾病影响和健康行为的重点，同时指出了非细菌群落、相互作用机制和对生态失调的长期影响的研究空白。

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引用次数: 0

LL-37 antimicrobial peptide: Molecular characterisation and its role in oral health and disease–A narrative review LL-37抗菌肽的分子特征及其在口腔健康和疾病中的作用

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2026-03-01 Epub Date: 2026-01-10 DOI: 10.1016/j.archoralbio.2026.106516

Drisya Soman , Jayanthi P , Mahesh CM , Rahul Radhakrishnan

Background

Antimicrobial peptides are integral components of the innate immune system and play a vital role in maintaining oral homeostasis. LL-37, the only human cathelicidin antimicrobial peptide, has gained increasing attention due to its broad-spectrum antimicrobial activity and diverse immunomodulatory functions within the oral cavity.

Objective

This narrative review aims to characterise the molecular structure, biological functions, and clinical relevance of LL-37 in oral health and disease.

Materials and methods

A comprehensive literature search was conducted using electronic databases, including PubMed, Scopus, and Google Scholar, to identify relevant in vitro, in vivo, and clinical studies evaluating the expression, mechanisms of action, and pathological implications of LL-37 in oral tissues.

Results

LL-37 is expressed by various oral tissues, including the gingival epithelium, salivary glands, and inflammatory cells, contributing to host defence against microbial challenges. In addition to its direct antimicrobial effects, LL-37 modulates inflammatory responses, promotes wound healing, and influences cellular proliferation and angiogenesis. Altered expression of LL-37 has been associated with the pathogenesis of several oral diseases, such as periodontitis, dental caries, endodontic infections, and oral squamous cell carcinoma.

Conclusion

LL-37 plays a multifaceted role in oral immunity by integrating antimicrobial, immunomodulatory, and tissue-regenerative functions. Enhanced understanding of its molecular characterisation and biological activities may support the development of LL-37 as a diagnostic biomarker and a potential therapeutic target in oral pathology.

微生物肽是先天免疫系统的组成部分，在维持口腔内环境平衡中起着至关重要的作用。LL-37是唯一一种人类抗菌肽，因其广谱抗菌活性和多种口腔免疫调节功能而受到越来越多的关注。目的本综述旨在描述LL-37在口腔健康和疾病中的分子结构、生物学功能和临床相关性。材料与方法利用PubMed、Scopus、谷歌Scholar等电子数据库进行全面的文献检索，寻找有关LL-37在口腔组织中的表达、作用机制及病理意义的体外、体内及临床相关研究。结果sll -37在多种口腔组织中表达，包括牙龈上皮、唾液腺和炎症细胞，有助于宿主防御微生物的攻击。除了其直接的抗菌作用，LL-37调节炎症反应，促进伤口愈合，并影响细胞增殖和血管生成。LL-37表达的改变与多种口腔疾病的发病机制有关，如牙周炎、龋齿、牙髓感染和口腔鳞状细胞癌。结论ll -37具有抗菌、免疫调节和组织再生功能，在口腔免疫系统中发挥着多方面的作用。对其分子特征和生物学活性的进一步了解可能有助于LL-37作为口腔病理诊断生物标志物和潜在治疗靶点的发展。

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引用次数: 0