Archives of oral biology最新文献

{"title":"Evaluation of the impact of postnatal maternal separation stress on enamel formation in an experimental murine model","authors":"Júlia I.T. Sousa ,&nbsp;Juliana L. Gonçalves ,&nbsp;Guido A. Marañón-Vásquez ,&nbsp;Roberta D. Leme ,&nbsp;Larissa S. Sales ,&nbsp;Fabrício K. Carvalho ,&nbsp;Alexandra M. Queiroz ,&nbsp;Alice Corrêa Silva-Sousa ,&nbsp;Manoel Damião de Sousa-Neto ,&nbsp;Francisco W.G. Paula-Silva","doi":"10.1016/j.archoralbio.2025.106487","DOIUrl":"10.1016/j.archoralbio.2025.106487","url":null,"abstract":"<div><h3>Background</h3><div>The adverse effects of postnatal maternal separation (MS) on offspring development are well documented; however, its specific impact on dental enamel formation remains inadequately explored. This study examines the effects of maternal-separation–induced toxic stress on dental enamel formation in a murine model. Methods: A total of 24 Wistar rat offspring (<em>Rattus norvegicus</em>) were utilized, divided into a control group (n = 12) and an MS group (n = 12). The MS group underwent 4 h of daily separation from their mothers from postnatal day 2 to day 21, while the control group remained undisturbed with their mothers. Throughout the experimental period, body weight, length, and key developmental milestones (including incisor eruption, eye opening, and ear opening) were monitored. At postnatal day 28, the animals were euthanized, and the incisors were collected for analysis. Incisor photographs were conducted for macroscopic evaluation. Volumetric assessment of the enamel layer was performed using micro-computed tomography (micro-CT) imaging. Microhardness testing quantified enamel resistance, while scanning electron microscopy (SEM) provided insights into the morphology and ultrastructure of incisor enamel. In addition, energy-dispersive X-ray spectroscopy (EDS) was performed to evaluate mineral content and identify possible compositional alterations in dental enamel. Data were statistically analyzed using Student's t-test (α = 0.05). Results: The findings revealed an increase in body weight within the MS group (<em>p</em> &lt; 0.05), while normal growth and developmental milestones exhibited no significant changes (<em>p</em> &gt; 0.05). No macroscopic alterations were observed in the dental enamel of incisors of MS group. SEM analysis of the incisors indicated that the enamel structure of incisors in the MS group maintained an organized arrangement of enamel rods, characterized by densely packed, elongated structures extending from the underlying dentin to enamel surface. Furthermore, structural integrity, microhardness, and mineral composition remained largely unaffected (<em>p</em> &gt; 0.05). Conclusion: Under the given experimental conditions, postnatal maternal separation does not significantly compromise dental enamel formation in rat offspring.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"183 ","pages":"Article 106487"},"PeriodicalIF":2.1,"publicationDate":"2025-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145881674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Odor characteristics and associated bacterial profiles in peri-implantitis using a novel odor measurement device","authors":"Tadahiro Kashiwamura ,&nbsp;Yusuke Taniguchi ,&nbsp;Ryutaro Ito ,&nbsp;Hirofumi Kido ,&nbsp;Kae Kakura ,&nbsp;Nao Suzuki","doi":"10.1016/j.archoralbio.2025.106486","DOIUrl":"10.1016/j.archoralbio.2025.106486","url":null,"abstract":"<div><h3>Objective</h3><div>This study assessed peri-implantitis risk by measuring odor using a novel device capable of identifying odorous components and bacteria specific to peri-implantitis.</div></div><div><h3>Design</h3><div>Implants from eight peri-implantitis patients and superstructures from 20 maintenance patients were evaluated using organoleptic testing and an odor detection device (nose@MEMS, I-PEX, Kyoto, Japan). In the maintenance group, 13 superstructures emitted odor, while 7 did not. Plaque samples were collected from peri-implant sites, and bacterial composition was analyzed via 16S rRNA sequencing.</div></div><div><h3>Results</h3><div>Principal component analysis of odor measurements obtained with the I-PEX system revealed that implants from peri-implantitis patients and odor-positive superstructures during maintenance formed a distribution distinct from odor-negative superstructures. Comparative analysis of bacterial composition between samples in the odor-associated region (Region A) and those including odor-negative superstructures (Region B) showed that Region A had a higher proportion of anaerobic bacteria, while Region B was enriched in commensal oral bacteria. Among 14 bacterial species previously linked to peri-implantitis, <em>Porphyromonas gingivalis</em>, <em>Tannerella forsythia</em>, and <em>Porphyromonas endodontalis</em> were significantly more abundant in Region A, whereas <em>Fretibacterium fastidiosum</em> and <em>Mogibacterium</em> were significantly lower.</div></div><div><h3>Conclusions</h3><div>The odor characteristics of peri-implantitis and odor-positive superstructures were similar and clearly distinguishable from odor-negative counterparts. The shared odor profile was associated with higher proportions of bacterial species previously linked to peri-implantitis. These findings imply that odor analysis using this novel device may provide a promising strategy for implant maintenance and peri-implantitis risk prediction.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"183 ","pages":"Article 106486"},"PeriodicalIF":2.1,"publicationDate":"2025-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145919421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insulin-like growth factor-binding protein 3 regulates collagen production via TGF-β-Smad2/3 pathway and osteogenic differentiation related with phosphorylation of Akt in human periodontal ligament-derived cell line","authors":"Shuxin Wang ,&nbsp;Hiromi Mitarai ,&nbsp;Hiroko Wada ,&nbsp;Ziqing Ran ,&nbsp;Asuka Yuda ,&nbsp;Akira Haraguchi ,&nbsp;Weihao Sun ,&nbsp;Jiawen Lin ,&nbsp;Hidefumi Maeda ,&nbsp;Naohisa Wada","doi":"10.1016/j.archoralbio.2025.106483","DOIUrl":"10.1016/j.archoralbio.2025.106483","url":null,"abstract":"<div><h3>Objective</h3><div>Insulin-like growth factor-binding protein 3 (IGFBP3) is a multifunctional protein involved in various cellular functions. However, the function of IGFBP3 in periodontal ligament (PDL) tissue remains unclear. In this study, we investigated the localization and function of IGFBP3 in PDL tissues and human PDL-derived cell line.</div></div><div><h3>Design</h3><div>Small interfering RNA (siRNA) and recombinant protein of IGFBP3 were used to examine the effect of IGFBP3 in human PDL-derived cell line. mRNA expression was determined by quantitative reverse transcription-polymerase chain reaction. Protein expression was determined using immunohistochemical staining, immunofluorescence staining, and Western blotting analyses. WST-1 and migration assays were used to analyze the effects on proliferation and migration. Collagen production was examined using picrosirius red staining. Calcium nodule formation was examined using Alizarin Red S staining.</div></div><div><h3>Results</h3><div>IGFBP3 was expressed in both mouse PDL tissues and human PDL-derived cell line (2–23 cells). Mechanical stretch and Transforming growth factor-beta 1 (TGF-β1) stimulation upregulated IGFBP3 expression in 2–23 cells. Knockdown of IGFBP3 using siRNA significantly suppressed PDL-related gene expression, collagen production, and inhibited Smad2/3 phosphorylation induced by TGF-β1, while IGFBP3 knockdown enhanced calcium chloride-induced osteogenic differentiation in 2–23 cells and activated Akt phosphorylation. Furthermore, treatment with exogenous rhIGFBP3 showed the opposite trend.</div></div><div><h3>Conclusions</h3><div>IGFBP3 plays a dual role in PDL homeostasis, by promoting collagen production and inhibiting osteogenic differentiation. IGFBP3 is involved in TGF-β-Smad2/3 pathway and related with phosphorylation of Akt, highlighting IGFBP3 as a potential therapeutic target for periodontal regeneration.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"183 ","pages":"Article 106483"},"PeriodicalIF":2.1,"publicationDate":"2025-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145881673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rutin (Vitamin P) attenuates oxidative stress, modulates cytokine profile, and preserves alveolar bone microarchitecture and density in a rat periodontitis model","authors":"Sami Barış Keskin ,&nbsp;Muhammed Mehdi Üremiş ,&nbsp;Yusuf Türköz ,&nbsp;Cüneyt Asım Aral","doi":"10.1016/j.archoralbio.2025.106485","DOIUrl":"10.1016/j.archoralbio.2025.106485","url":null,"abstract":"<div><h3>Objectives</h3><div>To evaluate the anti-inflammatory and antioxidant effects of rutin in experimental periodontitis.</div></div><div><h3>Design</h3><div>Wistar Albino rats were divided into four groups (n = 8 per group): 1) Healthy Control (HC), 2) Periodontitis (P), 3) P + Rutin 50 mg/kg (PR-50), and 4) P + Rutin 100 mg/kg (PR-100). Rutin was administered orally throughout the 14-day experimental period. Gingival levels of interleukin-1 beta (IL-1β), interleukin-10 (IL-10), glutathione peroxidase (GPX), malondialdehyde (MDA), and superoxide dismutase (SOD) and were measured using enzyme-linked immunosorbent assay, while oxidative stress index (OSI), total oxidant (TOS), and antioxidant status (TAS) were analysed spectrophotometrically. Alveolar bone was assessed by micro-computed tomography, including linear (ABL), volumetric (BV/TV), microarchitectural (BS/BV, Tb.Th, Tb.N, Tb.Sp), and densitometric (GV, HU, BMD) parameters.</div></div><div><h3>Results</h3><div>IL-1β/IL-10 ratio was lower, and IL-10 and GPX levels were higher in PR-100 group than in P group (p &lt; 0.05). SOD levels were higher in HC and PR-100 groups than in P group (p &lt; 0.001). OSI was highest in P (p &lt; 0.001). TOS was higher in P versus HC, whereas TAS was lower in PR-50 versus HC (p &lt; 0.05). ABL and BV/TV analyses showed significant bone preservation in both rutin groups compared to P (p &lt; 0.01). Microstructural and densitometric indices further confirmed less alveolar bone deterioration in rutin-treated rats.</div></div><div><h3>Conclusion</h3><div>Rutin exerts dose-dependent anti-inflammatory, antioxidant, and bone-protective effects in experimental periodontitis, suggesting its potential as an adjunctive therapeutic agent.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"183 ","pages":"Article 106485"},"PeriodicalIF":2.1,"publicationDate":"2025-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145771915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Submandibular saliva from male rats modulates osteogenic differentiation of bone marrow progenitor cells In Vitro","authors":"Gastón R. Troncoso ,&nbsp;María V. Gangoiti ,&nbsp;Javier Fernández-Solari ,&nbsp;Claudia E. Mohn ,&nbsp;María S. Molinuevo","doi":"10.1016/j.archoralbio.2025.106484","DOIUrl":"10.1016/j.archoralbio.2025.106484","url":null,"abstract":"<div><h3>Objective</h3><div>To investigate the effect of submandibular saliva from male rats on the proliferation and osteoblastic differentiation of bone marrow progenitor cells.</div></div><div><h3>Design</h3><div>Saliva was collected and pooled from adult male rats, and the total protein content was measured. Bone marrow progenitor cells (BMPC) from adult male rats were incubated with varying concentrations of salivary protein (ranging from 0 to 30 µg/mL) to assess cell proliferation. Osteoblastic differentiation was evaluated by measuring alkaline phosphatase activity, collagen type 1 production, mineral nodule formation, and molecular markers of differentiation after 7, 15, or 21 days of saliva exposure in a differentiating culture medium. The pro-inflammatory effects of saliva on BMPC were assessed by measuring tumor necrosis factor-alpha, interleukin 1 beta, prostaglandin E2, and matrix metalloproteinases 2 and 9.</div></div><div><h3>Results</h3><div>Low saliva concentration stimulated BMPC, promoting a pro-secretory and proliferative state. In contrast, higher concentration inhibited both processes under basal conditions. Furthermore, in osteogenic medium, saliva decreased alkaline phosphatase activity, collagen-1 production, and matrix mineralization in BMPC, demonstrating a dose- and time-dependent effect. Saliva also increased the secretion of interleukin 1β, tumor necrosis factor α, prostaglandin E2, and metalloproteinase activity in these cells, which inhibited osteoblastic differentiation.</div></div><div><h3>Conclusion</h3><div>Submandibular saliva has a biphasic effect on the osteogenic commitment of bone marrow progenitor cells, depending on the concentration and duration of exposure. This finding underscores the active role of saliva in the repair and regeneration of tooth sockets.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"182 ","pages":"Article 106484"},"PeriodicalIF":2.1,"publicationDate":"2025-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145776683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and eradication of mixed biofilms of Streptococcus mutans and Candida albicans using naringin: In vitro and in silico investigations","authors":"Chevuru Sai Shreya Reddy ,&nbsp;Lekha Sree Venkatesan ,&nbsp;Dhanraj Ganapathy,&nbsp;Palanivel Sathishkumar","doi":"10.1016/j.archoralbio.2025.106482","DOIUrl":"10.1016/j.archoralbio.2025.106482","url":null,"abstract":"<div><h3>Objective</h3><div>To identify a potential natural therapeutic agent to treat the mixed biofilms of <em>Streptococcus mutans</em> and <em>Candida albicans</em>, thereby preventing dental caries.</div></div><div><h3>Design</h3><div>The coexisting <em>S. mutans</em> and <em>C. albicans</em> was isolated from dental caries. Antimicrobial activity of various natural components was assessed against <em>S. mutans</em> and <em>C. albicans</em>. Antibiofilm efficacy of naringin was assessed against the biofilm of mixed <em>S. mutans</em> and <em>C. albicans</em>. The cell viability in mixed biofilm was determined using MTT assay and CLSM investigations. The molecular docking analysis of naringin with secreted aspartic proteinase (SAP2) of <em>C. albicans</em> and glucosyltransferase-I (GtfB) of <em>S. mutans</em> was performed using AutoDock and PyMOL tools. The biocompatibility of naringin was determined on human RBCs and HGF cells.</div></div><div><h3>Results</h3><div>The natural components such as curcumin, naringin, quercetin, morin, rutin, and hesperetin shows the antimicrobial effects against the clinical isolates <em>S. mutans</em> and <em>C. albicans</em>. Among these, naringin exhibits the significant antimicrobial effect against <em>S. mutans</em> (MIC:183 ± 5.70 µM), <em>C. albicans</em> (MIC:196 ± 0.00 µM), and their mixed culture (MIC:200 ± 10.00 µM). The MTT and CLSM results indicates that 2xMIC (400 µM) of naringin demonstrates the potential eradication of mature biofilm of mixed <em>S. mutans</em> and <em>C. albicans</em>. Naringin establishes the strong binding interaction with enzymes GtfB of <em>S. mutans</em> and SAP2 of <em>C. albicans</em>. The IC₅₀ value of naringin towards HGF cells was noted as 711.5 µM.</div></div><div><h3>Conclusions</h3><div>The flavonoid naringin demonstrates the effective and safe therapeutic potential to treat coexisting <em>S. mutans</em> and <em>C. albicans</em> biofilm virulence to prevent the dental caries.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"183 ","pages":"Article 106482"},"PeriodicalIF":2.1,"publicationDate":"2025-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145799797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the relationship between gut microbiota-metabolite axis and Jiawei Danxuan Koukang’s therapeutic effects in male rats with oral submucous fibrosis: A multi-omics analysis","authors":"Chenwei Wang ,&nbsp;Yuzhe Dai ,&nbsp;Yao Ye ,&nbsp;Qianqi Zeng ,&nbsp;Jin Tan","doi":"10.1016/j.archoralbio.2025.106480","DOIUrl":"10.1016/j.archoralbio.2025.106480","url":null,"abstract":"<div><h3>Objective</h3><div>This work aims to explore how Jiawei Danxuan Koukang (JDK) ameliorates Oral Submucous Fibrosis (OSF) via regulating the gut microbiota and its metabolites.</div></div><div><h3>Design</h3><div>We established an OSF rat model via oral mucosal arecoline injection. Rats were randomly divided into five groups: control, model, lycopene-treated, quercetin-treated, and JDK-treated. After intervention, we analyzed: serum metabolites by Liquid Chromatography-Mass Spectrometry -based untargeted metabolomics; gut microbiota profiling via 16S rDNA sequencing; oral mucosal histopathology and fibrosis using hematoxylin-eosin and Masson trichrome staining; expressions of fibrosis-related proteins (Transforming Growth Factor-β1 (TGF-β1), Collagen Type I Alpha 1 Chain (COL1A1)) by western blot; and cytokines (Interleukin-1β, Interleukin-6, Interleukin-10) in serum, oral and colon tissues via enzyme-linked immunosorbent assay.</div></div><div><h3>Results</h3><div>In the OSF model group, 120 serum metabolites were upregulated and 39 were down-regulated. When comparing the JDK group with the model group, 83 metabolites were upregulated and 132 were downregulated in the JDK group. Specifically, JDK targeted key metabolites: prostaglandins, leukotrienes, lysophosphatidylcholine, and 4-hydroxyproline. JDK also regulated two critical metabolic pathways: gly-cine-serine-threonine metabolism and tryptophan metabolism. JDK reduces the pro-inflammatory Ruminococcus and restores the butyrate-producing Lachnospiraceae_NK4A136_group. JDK downregulated Interleukin-1β and Interleukin-6 levels systemically and locally, concurrently increasing Interleukin-10, and reduced the expression of fibrosis-related proteins (TGF-β1, COL1A1) in the oral mucosa.</div></div><div><h3>Conclusions</h3><div>JDK alleviates OSF by normalizing inflammation and fibrosis-related metabolic pathways, linking gut microbiota remodeling to metabolic homeostasis.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"182 ","pages":"Article 106480"},"PeriodicalIF":2.1,"publicationDate":"2025-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145770178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Escitalopram exposure compromises osteogenic potential of human osteoblastic cells","authors":"Augusto Del Pintor Pasotti ,&nbsp;Rodrigo Mendes Ferreiro Girondo ,&nbsp;Bruno Haddad ,&nbsp;Marcelo Franchin ,&nbsp;Bruna Benso ,&nbsp;Rogerio Heládio Lopes Motta ,&nbsp;Henrique Ballassini Abdalla","doi":"10.1016/j.archoralbio.2025.106481","DOIUrl":"10.1016/j.archoralbio.2025.106481","url":null,"abstract":"<div><h3>Objective</h3><div>This study aimed to assess the impact of escitalopram on bone metabolism by evaluating its effects on cell viability and proliferation, wound-healing capacity, osteogenic activity, bone formation markers, and collagen deposition.</div></div><div><h3>Design</h3><div>The effects of escitalopram were studied on human osteoblastic SAOS-2 cells. Escitalopram (1–1000 µM) was tested in a dose–response curve. Cell viability was measured by MTT assay, and proliferation by hemocytometer counting. Cell migration was examined with the Scratch assay over 72 h. Osteogenic differentiation was assessed by gene expression of RUNX2, Osterix (Osx), bone sialoprotein (BSP), type I collagen (COL1), and osteocalcin (OCN) using RT-qPCR. Alkaline phosphatase (ALP) activity was analyzed at 4 and 8 days. Mineralization was determined by Alizarin Red staining (days 10, 14, 21). For last, Immunofluorescence was carried out for collagen 1 staining (days 3, 7 and 10).</div></div><div><h3>Results</h3><div>Escitalopram induced cytotoxicity in doses greater than 100 µM, reducing cell viability within 24 h. At non-toxic concentrations (≤30 µM), proliferation was enhanced in 30 µM after 7 days. Conversely, escilalopram reduced the migration capacity in a concentration-dependent manner. Moreover, the gene expression of RUNX2, OSX, BSP, COL1, and OCN were diminished when exposed to escitalopram. In the functional tests, escitalopram significantly decreases ALP activity at day 4, but not at day 8. Mineralization was dose-dependently impaired at 14 and 21 days. Collagen type I immunofluorescence exhibit weaker staining when escitalopram exposure.</div></div><div><h3>Conclusion</h3><div>Escitalopram compromises osteoblast differentiation, extracellular matrix formation, and migratory potential. These results provide mechanistic insight into the adverse skeletal effects of SSRIs and suggest the need for monitoring bone health in long-term users.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"182 ","pages":"Article 106481"},"PeriodicalIF":2.1,"publicationDate":"2025-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145783949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oral green and red propolis attenuate bone resorption and inflammation in experimental apical periodontitis","authors":"José Alex da Silva ,&nbsp;Letícia Cabrera Capalbo ,&nbsp;Renan Dal-Fabbro ,&nbsp;Romulo de Oliveira Sales-Junior ,&nbsp;Bharbara de Moura Pereira ,&nbsp;Edilson Ervolino ,&nbsp;João Eduardo Gomes-Filho ,&nbsp;Leopoldo Cosme-Silva","doi":"10.1016/j.archoralbio.2025.106479","DOIUrl":"10.1016/j.archoralbio.2025.106479","url":null,"abstract":"<div><h3>Objective</h3><div>To test whether systemic green or red propolis modulates inflammation and bone resorption in rat apical periodontitis (AP).</div></div><div><h3>Design</h3><div>Twenty-four male Wistar rats received AP induction in the first mandibular molars and were randomized to Control, Green propolis, or Red propolis (n = 8/group). Propolis (100 mg/kg in water) or vehicle was administered daily by gavage for 30 days. On day 30, periapical tissues were evaluated by micro-CT, histology (inflammatory score), and immunohistochemistry for RANKL, OPG, and TRAP-positive osteoclasts. Data were analyzed with Shapiro-Wilk, Kruskal-Wallis/Dunn, or one-way ANOVA/Tukey (α=0.05).</div></div><div><h3>Results</h3><div>Both propolis groups showed significantly less periapical bone resorption than the control group on micro-CT analysis (p &lt; 0.05). Histological evaluation revealed predominantly chronic inflammatory infiltrates, with significantly lower inflammation scores in the propolis-treated groups (p &lt; 0.05). Immunohistochemical analysis demonstrated a significant reduction in RANKL expression, an increase in OPG levels, and fewer TRAP-positive multinucleated cells in both propolis groups compared to the control (p &lt; 0.05). No significant differences were observed between green and red propolis.</div></div><div><h3>Conclusion</h3><div>Thirty days of systemic green or red propolis similarly attenuated periapical inflammation and osteoclast-mediated bone resorption in experimental AP, reflected by lower resorption volumes, reduced RANKL/TRAP, and higher OPG, indicating that propolis may serve as a host-modulatory adjunct to preserve periapical bone.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"182 ","pages":"Article 106479"},"PeriodicalIF":2.1,"publicationDate":"2025-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145733278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of phenotype switched Candida auris mono-culture and co-culture biofilms on the morphology, viability, and adhesion of hTERT TIGKs and ORL-48 cell lines","authors":"Mukarramah Zainal ,&nbsp;Nurul ‘Izzah Mohd Sarmin ,&nbsp;Mohammad Johari Ibrahim ,&nbsp;Nicola Cirillo ,&nbsp;Stuart G. Dashper ,&nbsp;Mohd Hafiz Arzmi","doi":"10.1016/j.archoralbio.2025.106478","DOIUrl":"10.1016/j.archoralbio.2025.106478","url":null,"abstract":"<div><h3>Objectives</h3><div>This study aims to determine the paracrine effects of <em>Candida auris</em> phenotypic switching in mono- and co-culture with <em>Staphylococcus aureus</em> on oral epithelial homeostasis and oncogenic progression in phenotypically normal (hTERT TIGKs) and malignant (ORL-48) oral keratinocytes.</div></div><div><h3>Design</h3><div><em>C. auris</em> switched phenotype was scored using Phloxine B, and mono- and co-culture biofilms with <em>S. aureus</em> were developed. hTERT TIGKs and ORL-48 cell lines were independently seeded into 6-well and 96-well plates for dispase and viability test, respectively. The oral cell lines were exposed to phenotypically switched <em>C. auris</em> mono- and co-culture biofilm test cell growth medium (TCGM) for 24 h. Outcomes included cell morphology, metabolic activity/viability (CCK-8), and cell–cell adhesion (dispase assay).</div></div><div><h3>Results</h3><div>Microscopic observation revealed that the biofilm induced damage and disrupted epithelial cell integrity in a paracrine manner. The mono- and co-culture TCGM suppressed the growth of normal cells while promoting the metabolic activity of cancer cells. The adhesion analysis of hTERT TIGKs indicated a strong intercellular cohesion, while ORL-48 cells downregulated intercellular adhesion and compromised cell-cell cohesion.</div></div><div><h3>Conclusion</h3><div><em>C. auris</em> biofilms promote the development of a malignant phenotype by regulating cell viability, promoting epithelial-mesenchymal transition, and adhesion in a switched generation-dependent manner.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"182 ","pages":"Article 106478"},"PeriodicalIF":2.1,"publicationDate":"2025-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145783979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}