Archives of oral biology最新文献_第8页

MIR375 hypermethylation as a prognostic predictor for head and neck squamous cell carcinoma: Insights from The Cancer Genome Atlas (TCGA) MIR375高甲基化作为头颈部鳞状细胞癌的预后预测因子：来自癌症基因组图谱（TCGA）的见解

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2026-01-01 Epub Date: 2025-10-22 DOI: 10.1016/j.archoralbio.2025.106437

Ana Carolina da Silva Lima , Ileana Gabriela Sanghez de Rubió , Shuting Lin , Carla M. Prado , Peng Qiu , Maria Aderuza Horst

Objective

To conduct exploratory bioinformatics analyses of the potential prognostic value of DNA methylation patterns in MIR375 regulatory regions and of expression levels of its encoded microRNA, hsa‑miR‑375 (miR-375), in head and neck squamous cell carcinoma (HNSCC).

Design

Data from 490 HNSCC samples were obtained from The Cancer Genome Atlas (TCGA), 50 of which were paired non-tumor surgical margins. Clinicopathological data, DNA methylation data from regions associated with MIR375, and miR-375 expression data were analyzed. Kaplan-Meier curves and Cox regression analyses were used to determine overall survival (OS) and disease-free survival (DFS) at 5 years in each group.

Results

Differentially methylated regions were associated with miR-375 expression in HNSCC. Specially, hypermethylation in N shore region was independently associated with poor OS and DFS. After adjusting for methylation patterns in other regions and miR-375 expression, these associations remained significant. DFS was also associated with methylation patterns in the enhancer region. After adjusting for confounding factors (the most robust model), hypermethylation in the N shore region was found to be an unfavorable predictor of OS and DFS. Clinicopathological and lifestyle factors also influenced the OS and DFS rates.

Conclusions

Methylation in DNA regions that may influence MIR375 transcription can be considered as predictors of OS and DFS in HNSCC, particularly those in the N shore region. This region should be a target for future investigations into MIR375 methylation pattern and its role in modulating miR-375 expression in HNSCC.

目的：对头颈部鳞状细胞癌（HNSCC）中MIR375调控区DNA甲基化模式及其编码的microRNA hsa‑miR‑375 （miR-375）表达水平的潜在预后价值进行探索性生物信息学分析。设计：490例HNSCC样本的数据来自癌症基因组图谱（TCGA），其中50例是配对的非肿瘤手术切缘。分析临床病理数据、MIR375相关区域的DNA甲基化数据和miR-375表达数据。采用Kaplan-Meier曲线和Cox回归分析确定各组5年总生存期（OS）和无病生存期（DFS）。结果：HNSCC中差异甲基化区域与miR-375表达相关。特别是，N岸区高甲基化与较差的OS和DFS独立相关。在调整了其他区域的甲基化模式和miR-375表达后，这些关联仍然显著。DFS还与增强子区域的甲基化模式有关。在调整了混杂因素（最稳健的模型）后，发现N岸区域的高甲基化是OS和DFS的不利预测因子。临床病理和生活方式因素也影响OS和DFS率。结论：可能影响MIR375转录的DNA区域甲基化可以被认为是HNSCC中OS和DFS的预测因素，特别是在N岸地区。该区域应该成为未来研究MIR375甲基化模式及其在HNSCC中调节miR-375表达中的作用的目标。

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引用次数: 0

Ganoderic acid A attenuates Porphyromonas gingivalis-induced adhesion molecule expression in gingival fibroblasts and tissues via inhibition of NLRP6 inflammasome activation 灵芝酸A通过抑制NLRP6炎性小体激活，减弱牙龈卟啉单胞菌诱导的牙龈成纤维细胞和组织中的粘附分子表达。

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2026-01-01 Epub Date: 2025-10-15 DOI: 10.1016/j.archoralbio.2025.106429

Yuanbo Wang , Jianru Liu , Xiangying Ouyang , Wenyi Liu , Peiying Lyu , Shengnan Zhang , Jing Guo

Objectives

This study aimed to elucidate the regulatory effects and mechanisms of Ganoderic acid A (GAA) on Porphyromonas gingivalis (P. gingivalis)-induced expression of adhesion molecules in human gingival fibroblasts (hGFs) and periodontal tissues, focusing on NLRP6 inflammasome modulation.

Design

HGFs were stimulated with P. gingivalis strain W83. The expression levels of NLRP6, caspase-1, IL-1β,IL-18 and adhesion molecules ICAM-1 & VCAM-1 were analyzed by RT-PCR, Western blotting and ELISA, with or without GAA pretreatment. To further explore the regulatory role of GAA on the NLRP6 inflammasome, NLRP6 overexpression assays were performed in hGFs, followed by assessment of ICAM-1 and VCAM-1 expressions. Additionally, a ligature-induced periodontitis mice model was established to evaluate the effects of GAA on NLRP6 expression and inflammatory cell infiltration in periodontal tissues.

Results

GAA exhibited no cytotoxicity toward hGFs at low concentrations (within 16 μM). GAA pretreatment significantly inhibited P. gingivalis-induced activation of the NLRP6 inflammasome. Beyond IL-1β and IL-18, it also reduced ICAM-1 and VCAM-1 expression at both mRNA and protein levels. Overexpression of NLRP6 increased the expression of NLRP6 inflammasome components and adhesion molecules, whereas GAA treatment effectively suppressed these elevations. Furthermore, GAA administration markedly downregulated NLRP6 expression and attenuated inflammatory cell infiltration and periodontal inflammation in the mice periodontitis model.

Conclusions

GAA attenuates P. gingivalis-induced expression of adhesion molecules by inhibiting NLRP6 inflammasome activation in gingival fibroblasts and periodontal tissues, thereby alleviating inflammatory cell infiltration. These findings suggest the potential of GAA as a therapeutic agent for the management of periodontal inflammation.

目的：研究灵芝酸A （GAA）对牙龈卟啉单胞菌（P. gingivalis）诱导的人牙龈成纤维细胞（hGFs）和牙周组织中粘附分子表达的调控作用及其机制，重点研究NLRP6炎症小体的调控作用。设计：用牙龈假单胞菌菌株W83刺激hgf。采用RT-PCR、Western blotting和ELISA检测GAA预处理前后NLRP6、caspase-1、IL-1β、IL-18和粘附分子ICAM-1、VCAM-1的表达水平。为了进一步探讨GAA对NLRP6炎症小体的调节作用，我们在hgf中进行NLRP6过表达测定，随后评估ICAM-1和VCAM-1的表达。建立结扎性牙周炎小鼠模型，观察GAA对牙周组织NLRP6表达及炎症细胞浸润的影响。结果：低浓度（16 μM以内）GAA对hGFs无细胞毒性。GAA预处理可显著抑制牙龈假单胞菌诱导的NLRP6炎性体的活化。除IL-1β和IL-18外，它还能在mRNA和蛋白水平上降低ICAM-1和VCAM-1的表达。NLRP6的过表达增加了NLRP6炎症小体成分和粘附分子的表达，而GAA治疗有效地抑制了这些升高。此外，GAA可显著下调小鼠牙周炎模型中NLRP6的表达，减轻炎症细胞浸润和牙周炎症。结论：GAA通过抑制NLRP6炎症小体在牙龈成纤维细胞和牙周组织中的活化，减弱牙龈假单胞菌诱导的粘附分子表达，从而减轻炎症细胞浸润。这些发现提示了GAA作为牙周炎症治疗药物的潜力。

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引用次数: 0

Novel missense mutations in the tumor necrosis factor domain of Ectodysplasin-A cause non-syndromic tooth agenesis in two Chinese families

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2026-01-01 Epub Date: 2025-10-27 DOI: 10.1016/j.archoralbio.2025.106441

Chengcan Yang , Nuo Xu , Xiaona Song , Kai Yang , Qian Gao

Objective

Mutations of the Ectodysplasin-A (EDA) gene are generally associated with syndromic tooth agenesis, yet their influence on non-syndromic tooth agenesis (NSTA) has not been thoroughly elucidated. This study aimed to investigate the genetic basis of NSTA and to identify pathogenic EDA mutations in two Chinese families using whole-exome sequencing, and explore the underlying mechanisms involved.

Design

Two unrelated individuals with NSTA and their families were enrolled in this study, and genomic DNA was extracted from peripheral blood samples. Whole-exome sequencing was performed, followed by variants detection and filtering to identify pathogenic genes and mutations. The identified genetic mutations were further validated by Sanger sequencing and confirmed to be absent in 300 unrelated healthy individuals. Conservation analysis, three-dimensional (3D) modeling, and electrostatic potential calculations were conducted accordingly.

Results

We identified a novel mutation c.827 G>T (p.Arg276Leu) and a previously reported mutation c.1069 C>T (p.Arg357Trp) in the EDA gene, with the latter showing previously unreported phenotypic characteristics. Both mutations are located in the tumor necrosis factor (TNF) domain of EDA, which is crucial for binding with ectodysplasin A receptor (EDAR). Conservation analysis indicated that these mutations are evolutionarily conserved across various species. In silico predictions and 3D modeling analyses suggested that these mutations may be pathogenic, potentially due to alterations in the hydrogen bonding and electrostatic potential, thereby affecting EDA-EDAR interaction.

Conclusions

Our findings broaden the mutation spectrum of EDA gene associated with NSTA by identifying a novel mutation and provide a basis for future genetic counseling.

目的：体外发育不良蛋白a （Ectodysplasin-A， EDA）基因突变通常与综合征性牙齿发育有关，但其对非综合征性牙齿发育（NSTA）的影响尚未完全阐明。设计：本研究招募了两名无血缘关系的NSTA患者及其家人，并从外周血样本中提取基因组DNA。进行全外显子组测序，然后进行变异检测和过滤以鉴定致病基因和突变。通过Sanger测序进一步验证了所鉴定的基因突变，并证实在300名不相关的健康个体中不存在。据此进行了守恒分析、三维（3D）建模和静电势计算。结果：我们在EDA基因中发现了一个新的突变C .827 G>T （p.a g276leu）和一个先前报道的突变C .1069 C>T (p.a g357trp)，后者显示出以前未报道的表型特征。这两个突变都位于EDA的肿瘤坏死因子（TNF）结构域，该结构域对于与外胞质异常蛋白A受体（EDAR）结合至关重要。保守性分析表明，这些突变在不同物种间具有进化保守性。计算机预测和3D建模分析表明，这些突变可能是致病的，可能是由于氢键和静电势的改变，从而影响EDA-EDAR相互作用。结论：我们的发现拓宽了与NSTA相关的EDA基因的突变谱，为未来的遗传咨询提供了基础。

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引用次数: 0

Traditional Chinese medicine Pudilan synergizes with smc gene to impair cell growth and exopolysaccharides synthesis of Streptococcus mutans 中药蒲地兰与smc基因协同作用抑制变形链球菌细胞生长和胞外多糖合成。

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2026-01-01 Epub Date: 2025-10-22 DOI: 10.1016/j.archoralbio.2025.106439

Hongyu Zhang, Yiting Cheng, Yalan Deng, Yingming Yang, Lei Lei, Tao Hu

Objective

This study examines the impact of Pudilan mouthwash in conjunction with the smc gene of Streptococcus mutans (S. mutans) on biofilm formation.

Design

We constructed the smc gene null mutant strains of S. mutans (Δsmc) via homologous recombination, and overexpression strains (smc^OE) and complementary strains (smc^Comp) through chemical transformation of smc recombinant plasmid. We then tested Pudilan's MIC and MBC on UA159 parent strains and smc mutants. Growth curves were used to analyze growth rates. Bacterial viability was assessed with Live/Dead Staining. Biofilm formation and structure were examined using crystal violet staining, confocal laser scanning microscopy, and scanning electron microscopy. Genes involved in exopolysaccharides (EPS) metabolism were quantified by qRT-PCR. Network pharmacology built a compound-target network, and molecular docking studied interactions between Pudilan's components and S. mutans proteins.

Results

The combination of Pudilan and knockout of smc gene exhibited a synergistic antibacterial effect on S. mutans. Results indicated that this pairing effectively inhibited the growth and viability of S. mutans, reduced biofilm biomass, and rearranged the structure of 8-hour and 24-hour biofilms. Additionally, Pudilan treatment downregulated gene expression related to water-soluble glucan in Δsmc. Compounds like (S)-cheilanthifoline in Pudilan may significantly influence dental plaque development by modulating GtfD activity.

Conclusions

This finding demonstrates that Pudilan enhances the inhibitory effect caused by smc deletion on S. mutans growth and viability, reduces biofilm EPS, and downregulates the expression of genes involved in water-soluble glucan synthesis.

目的：研究蒲地兰漱口水联合突变链球菌smc基因对生物膜形成的影响。设计：通过同源重组构建S. mutans的smc基因零突变株（Δsmc），通过smc重组质粒化学转化构建过表达株（smc^OE）和互补株（smc^Comp）。然后对UA159亲本株和smc突变株进行了Pudilan的MIC和MBC检测。生长曲线用于分析生长速率。采用活/死染色法评估细菌活力。采用结晶紫染色、激光共聚焦扫描显微镜和扫描电镜观察生物膜的形成和结构。采用qRT-PCR技术对外多糖（EPS）代谢相关基因进行定量分析。网络药理学构建化合物-靶点网络，分子对接研究蒲地兰成分与变形链球菌蛋白的相互作用。结果：蒲地兰联合敲除smc基因对变形链球菌具有协同抑菌作用。结果表明，这种配对有效抑制了变形链球菌的生长和生存能力，减少了生物膜的生物量，并重新排列了8小时和24小时生物膜的结构。此外，蒲地兰处理下调了Δsmc中水溶性葡聚糖相关基因的表达。蒲地兰中的(S)-cheilanthifoline等化合物可能通过调节GtfD活性显著影响牙菌斑的形成。结论：蒲地兰增强了smc缺失对变形链球菌生长和活力的抑制作用，降低了生物膜EPS，下调了水溶性葡聚糖合成相关基因的表达。

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引用次数: 0

Gingival crevicular fluid levels of soluble triggering receptor expressed on myeloid cells 1 in chronic periodontitis: A systematic review and meta-analysis 慢性牙周炎患者龈沟液中髓样细胞1可溶性触发受体表达水平的系统回顾和荟萃分析

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2026-01-01 Epub Date: 2025-10-25 DOI: 10.1016/j.archoralbio.2025.106440

Jingsong Kong, Weiqiu Jin, Min Wang, Fuhua Yan , Yanfen Li

Objectives

To compare gingival crevicular fluid (GCF) levels of soluble Triggering Receptor Expressed on Myeloid cells 1 (sTREM-1) between patients with chronic periodontitis (CP) and those with healthy periodontal (HP) status, and evaluate its predictive value for periodontal disease progression.

Design

Systematic review and meta-analysis.

Data sources

PubMed, MEDLINE, Cochrane, Web of Science, EMBASE, CNKI, and Wanfang were searched through December 2024. Manual searches were performed.

Eligibility criteria

Only studies reporting GCF sTREM-1 CP patients and HP controls were included. Clinical trials, cross-sectional, and observational studies were included.

Data extraction and synthesis

Two independent reviewers extracted data and assessed the quality. Sensitivity analyses ensured result stability. Quality assessment was performed according to the Newcastle-Ottawa Scale. Random-effects model calculated standardized mean differences (SMD)with 95 % CI via Review Manager software. Publication bias was evaluated using funnel plots and Egger's tests. The certainty of the evidence was assessed using the Grading of Recommendations, Assessment, Development and Evaluations (GRADE) approach.

Results

Among 128 screened studies, 9 articles met eligibility criteria of the systematic review, and 6 articles (147 CP patients, 116 HP controls) were included in the meta-analysis. GCF sTREM-1 levels were higher in the CP group than HP group (SMD = 0.70;

Z

= 3.25;

P

<0.001, 95 % CI).

Conclusions

This study found higher GCF sTREM-1 levels in CP patients compared to HP controls, suggesting potential as a diagnostic marker. However, small sample sizes and methodological variability limit the findings.

PROSPERO registration number

CRD42022326022

目的：比较慢性牙周炎（CP）患者和健康牙周（HP）患者龈沟液（GCF）可溶性髓样细胞触发受体1 （sTREM-1）水平，并评估其对牙周病进展的预测价值。设计：系统回顾和荟萃分析。数据来源：PubMed， MEDLINE, Cochrane, Web of Science， EMBASE， CNKI，万方检索截止到2024年12月。执行手动搜索。入选标准：仅纳入报告GCF sTREM-1 CP患者和HP对照的研究。包括临床试验、横断面研究和观察性研究。数据提取和综合：两名独立的审稿人提取数据并评估质量。敏感性分析确保了结果的稳定性。根据纽卡斯尔-渥太华量表进行质量评估。随机效应模型通过Review Manager软件计算标准化平均差异（SMD）， 95% % CI。采用漏斗图和Egger检验评估发表偏倚。采用推荐、评估、发展和评价分级（GRADE）方法评估证据的确定性。结果：在128项筛选的研究中，9篇文章符合系统评价的资格标准，6篇文章（147例CP患者，116例HP对照）被纳入meta分析。CP组GCF sTREM-1水平高于HP组（SMD = 0.70; Z = 3.25; p）结论：本研究发现CP患者GCF sTREM-1水平高于HP对照组，提示其有作为诊断标志物的潜力。然而，小样本量和方法的可变性限制了研究结果。普洛斯彼罗注册号：CRD42022326022。

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引用次数: 0

Patterns of circulating autoantibodies against desmoglein-1 and desmoglein-3 in the progression of oral lichen planus: A scoping review 口腔扁平苔藓发展过程中抗粘连蛋白-1和粘连蛋白-3循环自身抗体的模式：范围综述。

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2026-01-01 Epub Date: 2025-10-17 DOI: 10.1016/j.archoralbio.2025.106431

Andari Sarasati , Kadek Gede Putra Wibawa , Meircurius Dwi Condro Surboyo , Rene Luis Panico , Arvind Babu Rajendra Santosh , Adhistya Viany , Dewi Agustina , Diah Savitri Ernawati

Objective

The potential role of autoantibodies targeting desmosomal proteins, particularly desmoglein 1 (Dsg-1) and desmoglein 3 (Dsg-3), in modulating disease severity and epithelial integrity in oral lichen planus (OLP) remains a subject of growing interest. Distinct patterns of anti-Dsg-1 and anti-Dsg-3 autoantibodies across OLP subtypes may correlate with disease progression. This scoping review investigates the expression patterns of circulating anti-Dsg-1 and anti-Dsg-3 autoantibodies in reticular, erosive, and ulcerative forms of OLP to elucidate their potential roles in epithelial disruption and disease advancement.

Design

Following PRISMA guidelines, a systematic search of four electronic databases was conducted. Eligible studies included case reports, case series, case-control and cross-sectional studies that reported serum levels of anti-Dsg-1 and anti-Dsg-3 autoantibodies in OLP patients.

Results

Reticular-type OLP showed variable anti-Dsg-1 and anti-Dsg-3 autoantibody levels, with some patients testing positive while others were negative, indicating an inconsistent involvement of desmosomal autoimmunity in this milder form of the disease.

In erosive-type OLP, the presence of both autoantibodies indicated deeper epithelial damage and an active autoimmune response. Interestingly, ulcerative-type OLP consistently tested negative for both antibodies despite extensive epithelial breakdown, implying a potential shift from desmosomal autoimmunity to chronic inflammation–driven tissue destruction that could compromise human health.

Conclusion

These findings suggest a potential continuum in which reticular OLP may progress to erosive OLP, with Dsg-3 autoantibodies as a possible early marker of worsening pathology. Ulcerative OLP appears to represent a late-stage, inflammation-driven phenotype with minimal autoantibody involvement. Given the limited and heterogeneous data, these observations are preliminary and should be interpreted cautiously.

目的：针对桥粒蛋白的自身抗体，特别是桥粒蛋白1 （Dsg-1）和桥粒蛋白3 (Dsg-3)，在调节口腔扁平苔藓（OLP）疾病严重程度和上皮完整性方面的潜在作用仍然是一个越来越受关注的主题。不同OLP亚型的抗dsg -1和抗dsg -3自身抗体的不同模式可能与疾病进展相关。本综述研究了网状、糜烂和溃疡性OLP中循环抗dsg -1和抗dsg -3自身抗体的表达模式，以阐明它们在上皮破坏和疾病进展中的潜在作用。设计：按照PRISMA的指导方针，系统地检索了四个电子数据库。符合条件的研究包括报告OLP患者血清抗dsg -1和抗dsg -3自身抗体水平的病例报告、病例系列、病例对照和横断面研究。结果：网状型OLP显示抗dsg -1和抗dsg -3自身抗体水平变化，一些患者检测呈阳性，而另一些患者检测呈阴性，表明在这种较轻形式的疾病中，桥粒体自身免疫的参与不一致。在糜烂型OLP中，两种自身抗体的存在表明更深的上皮损伤和积极的自身免疫反应。有趣的是，尽管有广泛的上皮破坏，溃疡型OLP的两种抗体检测结果始终呈阴性，这意味着从桥粒体自身免疫到慢性炎症驱动的组织破坏的潜在转变，可能危及人类健康。结论：这些发现表明网状OLP可能发展为糜烂性OLP， Dsg-3自身抗体可能是病理恶化的早期标志。溃疡性OLP似乎代表了一种晚期，炎症驱动的表型，自身抗体参与最小。考虑到有限和异质性的数据，这些观察结果是初步的，应该谨慎解释。

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引用次数: 0

Assessment of nutrient canals in relation to jaw cysts, trabecular bone structure and dental regions: A CBCT study 评估营养管与颌骨囊肿、骨小梁结构和牙齿区域的关系：一项CBCT研究

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2026-01-01 Epub Date: 2025-10-17 DOI: 10.1016/j.archoralbio.2025.106433

Yasemin Merve Yılmaz , Defne Yalçın

Objective

To investigate the relationship of nutrient canals with jaw cysts, trabecular bone density, and dental regions using cone-beam computed tomography (CBCT).

Design

This retrospective study analyzed 120 CBCT images from 60 patients with unilateral jaw cysts. Nutrient canals were identified and localized on both the cyst-affected side and contralateral, unaffected side of the jaw for each patient. Alveolar trabecular structure was assessed by comparing Hounsfield Unit (HU) values between affected and unaffected sides. Statistical analyses included paired t-tests, Fisher’s exact test, and intra-observer reliability assessment.

Results

Nutrient canals were significantly more prevalent in cyst-affected jaw regions. Their presence was associated with areas exhibiting pericystic trabecular sclerosis, as reflected by elevated HU values. While nutrient canals were commonly observed in the anterior regions of both healthy and affected jaws, their presence in premolar and molar regions was restricted to cyst-affected areas.

Conclusions

Nutrient canals, typically found in the anterior jaws, appear to develop in posterior regions in association with jaw cysts, particularly in the presence of trabecular sclerosis. Their identification on CBCT scans is important for treatment planning and for minimizing potential surgical complications.

目的应用锥形束ct （cone-beam computed tomography， CBCT）研究营养管与颌骨囊肿、骨小梁骨密度及牙区之间的关系。本回顾性研究分析了60例单侧颌囊肿患者的120张CBCT图像。在每个患者的受囊肿影响的一侧和对侧未受影响的一侧颌骨上确定并定位营养管。通过比较患侧和未患侧的Hounsfield Unit （HU）值来评估肺泡小梁结构。统计分析包括配对t检验、Fisher精确检验和观察者内部信度评估。结果在患囊肿的颌区，营养管明显增多。它们的存在与表现为囊周小梁硬化的区域有关，这可以通过HU值升高来反映。虽然营养管通常在健康和患病颌骨的前区观察到，但它们在前磨牙和磨牙区域的存在仅限于囊肿影响区域。结论：在颌骨囊肿患者中，特别是在小梁硬化患者中，通常在前颚发现的三通管出现在下颌后部。在CBCT扫描上识别它们对于治疗计划和减少潜在的手术并发症非常重要。

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引用次数: 0

Bacterial extracellular vesicles from Porphyromonas gingivalis – proteomic profile and influence on oral fibroblasts 牙龈卟啉单胞菌的细菌胞外囊泡-蛋白质组学特征及其对口腔成纤维细胞的影响。

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2026-01-01 Epub Date: 2025-10-15 DOI: 10.1016/j.archoralbio.2025.106424

Helene Rygvold Haugsten , Anne Karin Kristoffersen , Morten Enersen , Bernd Thiede , Tine M. Søland , Trude M. Haug , Hilde Kanli Galtung

Objectives

In this study, we aimed to explore the protein content of bacterial extracellular vesicles (bEVs) from three strains of Porphyromonas gingivalis, an anaerobic gram-negative bacterium closely associated with periodontitis. Additionally, we aimed to investigate the effects of bEV uptake on human oral fibroblasts, to better understand the role of these vesicles in the pathogenesis of periodontitis.

Design

Liquid chromatography-mass spectrometry was performed on bEVs from three strains of P. gingivalis with different virulence: ATCC 33277, A7A1–28, and W83. Three key proteins associated with virulence were validated using dot blot. Proliferation and viability of human oral fibroblasts after incubation with bEVs were assessed at different timepoints and different concentrations of bEVs.

Results

A total of 307 proteins were identified in the bEVs from the three strains of P. gingivalis. Among those were several relevant for virulence, for example gingipains, fimbriae, and peptidyl-arginine deiminase (PPAD). When oral fibroblasts were exposed to bEVs from A7A1–28 or W83, proliferation was significantly affected after 12 and 24 h at one bEV concentration. Furthermore, viability was significantly affected by bEVs from W83 at several timepoints and bEV concentrations. bEVs from ATCC 33277 did not induce any significant changes.

Conclusion

Several proteins associated with virulence were detected in bEVs from all three strains of P. gingivalis, with varying abundance. Uptake of these bEVs can influence human cells, as exemplified by changes in viability and proliferation.

目的：在本研究中，我们旨在探讨三株牙龈卟啉单胞菌（一种与牙周炎密切相关的革兰氏阴性厌氧菌）的细菌细胞外囊泡（bEVs）的蛋白质含量。此外，我们旨在研究bEV摄取对人口腔成纤维细胞的影响，以更好地了解这些囊泡在牙周炎发病机制中的作用。设计：采用液相色谱-质谱法对三株不同毒力的牙龈假单胞菌ATCC 33277、A7A1-28和W83的bev进行分析。3个与毒力相关的关键蛋白采用点印迹法进行了验证。研究了不同时间点、不同浓度bev对人口腔成纤维细胞增殖和活力的影响。结果：从3株牙龈假单胞菌的bev中共鉴定出307个蛋白。其中有几个与毒力有关，例如牙龈痛、菌毛和肽精氨酸脱亚胺酶（PPAD）。当口腔成纤维细胞暴露于A7A1-28或W83的bEV时，在一个bEV浓度下，12和24 h后，增殖受到显著影响。此外，在不同时间点和不同浓度下，W83的bEV对细胞活力有显著影响。ATCC 33277的纯电动汽车没有引起任何显著的变化。结论：在三株牙龈假单胞菌的bev中均检测到几种与毒力相关的蛋白，且丰度不同。这些bev的摄取可以影响人类细胞，如活力和增殖的变化。

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引用次数: 0

Optimization and evaluation of antibacterial extracts from Acacia nilotica, Psidium guajava, Syzygium aromaticum, and Salvadora persica to inhibit dental caries 金合欢、番石榴、香薷、桃金娘抑菌提取物抑龋效果的优化与评价。

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2026-01-01 Epub Date: 2025-10-14 DOI: 10.1016/j.archoralbio.2025.106426

Chamundeswari Kandasamy , Arunadevi Baladhandapani , Renuka Devi Ponnuswamy , Hemananthan Eswaran , Vinodhini Karthikeyan , Saranya Rajendran

Objective

Dental caries is a significant global issue, which affects approximately 44 % of the global population. The objective of this research is to extract crude phytochemicals from Psidium guajava, Acacia nilotica, Syzygium aromaticum, and Salvadora persica and also optimizing using RSM. Therefore, this aims to maximize the antibacterial potential of the extracts at effective inhibition of dental caries

Method

The crude extracts from the plants samples, with varying extraction parameters, such as ethanol concentrations, temperatures, and solid-solvent ratios was done using Soxhlet method and antibacterial activity was determined through well and disc diffusion methods along with optimization was performed by Response Surface Methodology (RSM).

Results

The results depict that Psidium guajava exhibited the strongest antibacterial activity against S. mutans and Lactobacillus, with inhibition zones of 36 mm & 20 mm and 32 mm & 24 mm for both well and disc diffusion. Similarly, Syzygium aromaticum also shows slight stronger inhibition zones, Acacia nilotica exhibited moderate activity and in contrast, Salvadora persica showed limited activity. RSM optimization was validated and with well fitted quadratic models R² values ranging from 0.69 to 0.79. Predicted and experimental values are in close agreement between with differences below 0.5 %.

Conclusion

These findings suggest that P. guajava, S. aromaticum and A. nilotica have strong potential as natural antibacterial agents in oral care formulations.

目的：龋齿是一个重要的全球性问题，影响了全球约44% %的人口。本研究的目的是提取番石榴、金合欢、香合木和木耳的粗化学成分，并对其进行RSM优化。方法：采用索氏法提取不同提取参数（乙醇浓度、温度、固液比）的植物粗提物，采用孔法测定其抑菌活性，并采用响应面法（RSM）进行优化。结果：瓜石榴对变形链球菌和乳酸菌的抑菌活性最强，孔扩散区和盘扩散区分别为36 mm和20 mm和32 mm和24 mm。同样，Syzygium aromaticum也表现出稍强的抑制区，Acacia nilotica表现出中等的活性，而Salvadora persica则表现出有限的活性。二次模型拟合良好，R²值在0.69 ~ 0.79之间。预测值与实验值非常吻合，误差在0.5 %以下。结论：番石榴、香薷和尼罗花在口腔护理制剂中具有很强的天然抗菌潜力。

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引用次数: 0

Accuracy of dental age estimation methods in children with chromosomal syndromes: A systematic review and network meta-analysis 染色体综合征儿童牙龄估计方法的准确性：系统回顾和网络荟萃分析

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2026-01-01 Epub Date: 2025-11-05 DOI: 10.1016/j.archoralbio.2025.106445

Ilham Wan Mokhtar , Norsaima Ismail , Mohd Yusmiaidil Putera Mohd Yusof

Objective

The study aims to systematically examine the accuracy of the application of different dental age estimation (DAE) methods on children with chromosomal syndromes and evaluate its performance based on various populations and regions.

Design

A systematic search of the literature applying the PRISMA-NMA-compliant method was conducted using the databases PubMed, Web of Science, Science Direct, Google Scholar, and Scopus between 1981 and 2020; that estimate dental development of children with chromosomal syndrome based on populations, intervention, comparisons, and outcomes (PICO) search strategy identified. The literature quality was assessed using QUADAS-2. A network meta-analysis was performed to compare the effects of different chromosomal syndromes on the accuracy of estimated dental age (DA). Mean differences were calculated for difference of DA to chronological age (CA) by using the random effects model.

Results

From 60 titles retrieved utilising a standardized search strategy, 45 titles met the qualitative analysis criteria, and 28 titles qualified for the quantitative analysis requirements. Twenty-four comparative studies and twenty-one non-comparative studies suggested five DAE methods within this population used in combination or alone, namely Demirjian, Willems, Nolla, Haavikkoo, and London Atlas. Down’s syndrome is the highest contributing literature relating to DAE and chromosomal syndrome.

Conclusions

Underestimations were seen in Amelogenesis Imperfecta and Osteogenesis Imperfecta for Demirjian method. Other methods were lacking primary studies to make a reliable inference. This suggests that no single dental age estimation (DAE) method works consistently across diverse syndromes, emphasizing the importance of personalized approaches in clinical and forensic settings. Publication bias was minor, indicating solid findings.

目的系统考察不同牙龄估计（DAE）方法在染色体综合征患儿中应用的准确性，并基于不同人群和地区评价其效果。采用符合prisma - nma标准的方法，对1981 - 2020年PubMed、Web of Science、Science Direct、谷歌Scholar和Scopus等数据库的文献进行了系统检索；基于人群、干预、比较和结果（PICO）搜索策略来估计染色体综合征儿童的牙齿发育。采用QUADAS-2评价文献质量。进行网络荟萃分析，比较不同染色体综合征对估计牙龄（DA）准确性的影响。采用随机效应模型计算DA与实足年龄（CA）差异的均值差异。结果通过标准化检索策略检索到的60篇文献中，45篇文献符合定性分析标准，28篇文献符合定量分析要求。24项比较研究和21项非比较研究建议在该人群中联合或单独使用5种DAE方法，即Demirjian、Willems、Nolla、Haavikkoo和London Atlas。唐氏综合症是DAE和染色体综合征相关文献中贡献最大的。结论Demirjian法对无骨发育不全和成骨发育不全存在低估。其他方法缺乏初步研究，无法做出可靠的推断。这表明，没有单一的牙龄估计（DAE）方法在不同的综合征中始终有效，强调了个性化方法在临床和法医环境中的重要性。发表偏倚较小，表明研究结果可靠。

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引用次数: 0