Archives of oral biology最新文献_第8页

Is epithelial-mesenchymal transition related to the biological behavior of salivary gland neoplasms? 上皮-间质转化与唾液腺肿瘤的生物学行为有关吗？

IF 3 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2024-05-29 DOI: 10.1016/j.archoralbio.2024.106017

Débora Frota Colares , Nara Régia da Silva Domingos , Rodrigo Porpino Mafra , Leorik Pereira da Silva , Leão Pereira Pinto , Lélia Batista de Souza

Objective

To evaluate and compare the expression of E-cadherin, Snail1 and Twist1 in pleomorphic adenomas (PAs), adenoid cystic carcinomas (AdCCa) and carcinoma ex-pleomorphic adenomas (CaexPA) of salivary glands, as well as investigate possible associations with clinicopathological parameters.

Study design

E-cadherin, Snail1 and Twist1 antibody immunostaining were analyzed semiquantitatively in 20 PAs, 20 AdCCas and 10 CaexPAs. Cases were classified as low and high expression for analysis of the association with clinicopathological parameters.

Results

Compared to PAs, AdCCas and CaexPAs exhibited higher nuclear expression of Snail1 (p = 0.021 and p = 0.028, respectively) and Twist1 (p = 0.009 and p = 0.001). Membranous and cytoplasmic expression of E-cadherin were positively correlated in PAs, AdCCas and CaexPAs (r = 0.645, p = 0.002; r = 0.824, p < 0.001; r = 0.677, p = 0.031). In PAs, positive correlation was found between nuclear expression of Snail1 and membrane expression of E-cadherin (r = 0.634; p = 0.003), as well as between nuclear expression of Snail1 and Twist1 (r = 0.580; p = 0.007). Negative correlations were detected between membrane expression of E-cadherin and cytoplasmic expression of Snail1 in AdCCas (r = − 0.489; p = 0.029).

Conclusions

E-cadherin, Twist1, and Snail1 may participate in modulating events related to cell differentiation and adhesion in PAs and to biological behavior in AdCCas and CaexPAs, which indicates the involvement of EMT in these processes. Furthermore, the expression of these proteins in these carcinomas may reflect the plasticity feature of EMT.

目的评估和比较唾液腺多形性腺瘤（PA）、腺样囊性癌（AdCCa）和前多形性腺瘤癌（CaexPA）中 E-cadherin、Snail1 和 Twist1 的表达情况，并研究其与临床病理参数的可能关系。研究设计对20个PA、20个AdCCas和10个CaexPA的E-cadherin、Snail1和Twist1抗体免疫染色进行半定量分析。结果与 PAs 相比，AdCCas 和 CaexPAs 的 Snail1（p = 0.021 和 p = 0.028）和 Twist1（p = 0.009 和 p = 0.001）的核表达更高。在 PAs、AdCCas 和 CaexPAs 中，E-cadherin 的膜和细胞质表达呈正相关（r = 0.645，p = 0.002；r = 0.824，p < 0.001；r = 0.677，p = 0.031）。在 PAs 中，发现 Snail1 的核表达与 E-cadherin 的膜表达呈正相关（r = 0.634；p = 0.003），Snail1 的核表达与 Twist1 呈正相关（r = 0.580；p = 0.007）。结论E-cadherin、Twist1 和 Snail1 可能参与调节 PA 中与细胞分化和粘附有关的事件以及 AdCCas 和 CaexPAs 中与生物学行为有关的事件，这表明 EMT 参与了这些过程。此外，这些蛋白在这些癌肿中的表达可能反映了 EMT 的可塑性特征。

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引用次数: 0

Evergrowing incisors of diprotodont marsupials record age and life history 双齿有袋类动物不断生长的门齿记录着年龄和生活史

IF 3 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2024-05-29 DOI: 10.1016/j.archoralbio.2024.106018

William M.G. Parker , Justin W. Adams , Eliza J. Campbell , Graeme Coulson , Gordon D. Sanson , Alistair R. Evans

Objective

Tooth growth and wear are commonly used tools for determining the age of mammals. The most speciose order of marsupials, Diprotodontia, is characterised by a pair of procumbent incisors within the lower jaw. This study examines the growth and wear of these incisors to understand their relationship with age and sex.

Design

Measurements of mandibular incisor crown and root length were made for two sister species of macropodid (kangaroos and wallabies); Macropus giganteus and Macropus fuliginosus. Histological analysis examined patterns of dentine and cementum deposition within these teeth. Broader generalisability within Diprotodontia was tested using dentally reduced Tarsipes rostratus – a species disparate in body size and incisor function to the studied macropodids.

Results

In the macropodid sample it is demonstrated that the hypsodont nature of these incisors makes measurements of their growth (root length) and wear (crown length) accurate indicators of age and sex. Model fitting finds that root growth proceeds according to a logarithmic function across the lifespan, while crown wear follows a pattern of exponential reduction for both macropodid species. Histological results find that secondary dentine deposition and cementum layering are further indicators of age. Incisor measurements are shown to correlate with age in the sample of T. rostratus.

Conclusions

The diprotodontian incisor is a useful tool for examining chronological age and sex, both morphologically and microstructurally. This finding has implications for population ecology, palaeontology and marsupial evolution.

目的牙齿的生长和磨损是确定哺乳动物年龄的常用工具。有袋类中最特殊的类群--双齿兽，其下颌有一对平卧的门齿。本研究对这对门齿的生长和磨损情况进行了研究，以了解它们与年龄和性别的关系。设计对巨齿类（袋鼠和小袋鼠）的两个姊妹物种--巨齿象和巨齿虎进行了下颌门齿齿冠和齿根长度的测量。组织学分析研究了这些牙齿的牙本质和骨水泥沉积模式。结果在巨齿类样本中，这些门齿的半齿性使得对其生长（齿根长度）和磨损（齿冠长度）的测量成为年龄和性别的准确指标。模型拟合结果表明，牙根的生长在整个生命周期中都是按照对数函数进行的，而牙冠的磨损在两个巨足类动物中都是按照指数减少的模式进行的。组织学结果发现，继发性牙本质沉积和骨水泥层是年龄的进一步指标。结论从形态学和显微结构上看，双齿兽门齿是研究年代年龄和性别的有用工具。这一发现对种群生态学、古生物学和有袋类的进化具有重要意义。

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引用次数: 0

Dental disease in a 15th-17th centuries skeletal sample of enslaved Africans (Lagos, Portugal) 15-17 世纪被奴役非洲人骨骼样本中的牙病（葡萄牙拉各斯）

IF 3 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2024-05-29 DOI: 10.1016/j.archoralbio.2024.106015

Sofia N. Wasterlain , Ana I. Rufino , Maria Teresa Ferreira

Objective

Dental disease is frequently used as a proxy for diet and overall health of individuals of past populations. The aim of this study is to investigate dental disease in a sample of enslaved African individuals recovered from an urban dump (15th-17th centuries) in Lagos, Portugal.

Design

In all, 81 African individuals (>12 years old) were analysed (19 males, 49 females, and 13 of unknown sex), in a total of 2283 alveoli, 2061 teeth, and 2213 interdental septa. Analysed oral pathologies include dental caries, periodontal disease, and ante-mortem tooth loss. Dental wear was also recorded.

Results

Dental caries affected 52.0 % of the teeth, although only 31.9 % were cavitated lesions. In all, 96.3 % of the individuals presented at least one cariogenic lesion. Gingivitis and periodontitis were recorded in 56.7 % and 19.0 % of the septa, respectively. Only one male individual had all septal areas healthy. Ante-mortem tooth loss was recorded in 38.3 % of the individuals, in a total of 96 teeth lost (4.2 %). Regarding occlusal wear, 70.8 % of the surfaces were recorded with grades 1–3.

Conclusions

The frequencies of the oral pathological conditions observed may not only reflect a cariogenic diet (rich in starches and with a high frequency of meals) but also the conditions during the maritime voyage of the first victims of the North Atlantic slave trade (xerostomia due to lack of water, sea sickness and vomiting, vitamin C deficiency, poor hygiene), and also the impact intentional dental modifications had on the dentitions.

目的牙齿疾病经常被用来代表过去人群的饮食习惯和整体健康状况。本研究旨在调查从葡萄牙拉各斯的一个城市垃圾场（15-17 世纪）中发现的非洲奴隶样本中的牙科疾病。设计共分析了 81 名非洲人（12 岁）（19 名男性、49 名女性和 13 名性别未知者），共计 2283 个牙槽骨、2061 颗牙齿和 2213 个齿间隔膜。分析的口腔病变包括龋齿、牙周病和死前牙齿脱落。结果52.0%的牙齿患有龋齿，但只有31.9%为龋坏。总之，96.3%的人至少有一处龋齿病变。牙龈炎和牙周炎分别在 56.7% 和 19.0% 的牙齿隔中出现。只有一名男性个体的所有隔区都是健康的。据记录，38.3%的人死前牙齿脱落，共有 96 颗牙齿脱落（4.2%）。在咬合面磨损方面，70.8%的牙齿表面磨损程度为 1-3 级。结论所观察到的口腔病变频率不仅可能反映了致龋饮食（富含淀粉且进餐频率高），还可能反映了北大西洋奴隶贸易首批受害者在海上航行期间的状况（缺水导致的口腔干燥症、晕船和呕吐、维生素 C 缺乏、卫生条件差），以及有意对牙齿进行改造对牙齿造成的影响。

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引用次数: 0

Intermittent compressive force regulates matrix metalloproteinases and tissue inhibitors of metalloproteinases expression in human periodontal ligament cells 间歇压缩力可调节人牙周韧带细胞中基质金属蛋白酶和组织金属蛋白酶抑制剂的表达。

IF 3 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2024-05-27 DOI: 10.1016/j.archoralbio.2024.106011

Novena Dameria Pakpahan , Maythwe Kyawsoewin , Jeeranan Manokawinchoke , Worachat Namangkalakul , Chutimon Termkwancharoen , Hiroshi Egusa , Phoonsuk Limraksasin , Thanaphum Osathanon

Objective

This study aims to evaluate the effects of intermittent compressive force (ICF) on the expression of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) by human periodontal ligament cells (hPDLCs).

Design

hPDLCs were subjected to ICF with a magnitude of 1.5 g/cm² and loaded for 24 h. mRNA and protein expression of several MMPs and TIMPs were assessed using RT-PCR and ELISA analyses. An inhibitor of TGF-β (SB431542) was used to assess a possible role of TGF-β in the expression of MMPs and TIMPs under ICF.

Results

mRNA and protein analyses showed that ICF significantly induced expression of TIMP1 and TIMP3, but decreased expression of MMP1. Incubation with the TGF-β inhibitor and applied to ICF showed a downregulation of TIMP3, but expression of MMP1 was not affected.

Conclusion

ICF is likely to affect ECM homeostasis by hPDLCs by regulating the expression of MMP1 and TIMPs. Moreover, TGF-β1 regulated expression of TIMP3. These findings suggest ICF may decrease the degradation of ECM and may thus be essential for maintaining PDL homeostasis.

研究目的设计：将人牙周韧带细胞（hPDLCs）置于1.5 g/cm2的间歇压缩力（ICF）作用下并负载24 h，使用RT-PCR和ELISA分析评估几种MMPs和TIMPs的mRNA和蛋白表达。结果：mRNA 和蛋白质分析表明，ICF 显著诱导了 TIMP1 和 TIMP3 的表达，但降低了 MMP1 的表达。用 TGF-β 抑制剂孵育并应用于 ICF 显示 TIMP3 的表达下调，但 MMP1 的表达未受影响：结论：ICF 可能通过调节 MMP1 和 TIMPs 的表达来影响 hPDLC 的 ECM 稳态。此外，TGF-β1 还能调节 TIMP3 的表达。这些研究结果表明，ICF 可减少 ECM 的降解，因此可能对维持 PDL 的稳态至关重要。

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引用次数: 0

Salivary exosomal microRNA profile as biomonitoring tool for diagnosis and prognosis of patients with head and neck squamous cell carcinoma: a systematic review 唾液外泌体microRNA图谱作为头颈部鳞状细胞癌患者诊断和预后的生物监测工具：系统综述

IF 3 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2024-05-27 DOI: 10.1016/j.archoralbio.2024.106012

Lorenzo Sanesi , Giorgio Mori , Giuseppe Troiano , Andrea Ballini , Felice Valzano , Mario Dioguardi , Lorenzo Lo Muzio , Marco Magalhaes , Vito Carlo Alberto Caponio

Objective

Exosomes are extracellular vesicles found in saliva and other body fluids. These vesicles range in size from 30 to 150 nm and play a crucial role in intercellular communication, transporting different biomolecules, actively targeting cells. These vesicles regulate both physiological and pathological processes within recipient cells. MicroRNAs (miRs) are transported within exosomes and are delivered to target cells where they influence signaling pathways, taking on a crucial regulatory role in oncogenesis; for example, they are implicated in progression and infiltration of various cancers, such as head and neck squamous cell carcinoma (HNSCC).

Material and methods

A systematic literature search based on specific keywords, according to the PRISMA guidelines, was carried out on PubMed, Web of Science, Scopus, and Google Scholar. Only original articles were selected during this review. The risk of bias was assessed by QUADAS-2.

Results

At the end of the selection process 9 articles were included. In these studies, 41 miRs showed differential expression between healthy subjects and patient with HNSCC. The techniques varied among studies for the extraction and analysis of exosomal miRs. We presented also salivary exosomal miRs pathways, to give insights about pathogenetic mechanisms.

Conclusions

Exosomal microRNA are promising biomarkers for HNSCC detection. MiR-10b-5p, miR-486–5p, miR-24–3p, miR-412–3p, and miR-512–3p are the most promising markers applicable to diagnostics, while miR-1307–5p and miR-519c–3p resulted overexpressed and correlated to worse survival outcomes.

目的体外小泡是存在于唾液和其他体液中的细胞外小泡。这些囊泡的大小从 30 纳米到 150 纳米不等，在细胞间通信、运输不同的生物大分子、主动靶向细胞等方面发挥着重要作用。这些囊泡调节受体细胞内的生理和病理过程。微RNA（miRs）在外泌体中运输，并被输送到靶细胞，影响信号通路，在肿瘤发生过程中发挥重要的调控作用；例如，它们与头颈部鳞状细胞癌（HNSCC）等多种癌症的进展和浸润有关。材料与方法根据PRISMA指南，基于特定关键词在PubMed、Web of Science、Scopus和谷歌学术网上进行了系统的文献检索。本次综述只选择了原创文章。结果在筛选过程结束后，共纳入了 9 篇文章。在这些研究中，有 41 个 miRs 在健康受试者和 HNSCC 患者之间有差异表达。不同研究提取和分析外泌体 miRs 的技术各不相同。我们还介绍了唾液外泌体 miRs 的表达途径，以便深入了解病理机制。miR-10b-5p、miR-486-5p、miR-24-3p、miR-412-3p和miR-512-3p是最有希望用于诊断的标记物，而miR-1307-5p和miR-519c-3p则导致过表达，并与较差的生存结果相关。

{"title":"Salivary exosomal microRNA profile as biomonitoring tool for diagnosis and prognosis of patients with head and neck squamous cell carcinoma: a systematic review","authors":"Lorenzo Sanesi , Giorgio Mori , Giuseppe Troiano , Andrea Ballini , Felice Valzano , Mario Dioguardi , Lorenzo Lo Muzio , Marco Magalhaes , Vito Carlo Alberto Caponio","doi":"10.1016/j.archoralbio.2024.106012","DOIUrl":"https://doi.org/10.1016/j.archoralbio.2024.106012","url":null,"abstract":"<div><h3>Objective</h3><p>Exosomes are extracellular vesicles found in saliva and other body fluids. These vesicles range in size from 30 to 150 nm and play a crucial role in intercellular communication, transporting different biomolecules, actively targeting cells. These vesicles regulate both physiological and pathological processes within recipient cells. MicroRNAs (miRs) are transported within exosomes and are delivered to target cells where they influence signaling pathways, taking on a crucial regulatory role in oncogenesis; for example, they are implicated in progression and infiltration of various cancers, such as head and neck squamous cell carcinoma (HNSCC).</p></div><div><h3>Material and methods</h3><p>A systematic literature search based on specific keywords, according to the PRISMA guidelines, was carried out on PubMed, Web of Science, Scopus, and Google Scholar. Only original articles were selected during this review. The risk of bias was assessed by QUADAS-2.</p></div><div><h3>Results</h3><p>At the end of the selection process 9 articles were included. In these studies, 41 miRs showed differential expression between healthy subjects and patient with HNSCC. The techniques varied among studies for the extraction and analysis of exosomal miRs. We presented also salivary exosomal miRs pathways, to give insights about pathogenetic mechanisms.</p></div><div><h3>Conclusions</h3><p>Exosomal microRNA are promising biomarkers for HNSCC detection. MiR-10b-5p, miR-486–5p, miR-24–3p, miR-412–3p, and miR-512–3p are the most promising markers applicable to diagnostics, while miR-1307–5p and miR-519c–3p resulted overexpressed and correlated to worse survival outcomes.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"165 ","pages":"Article 106012"},"PeriodicalIF":3.0,"publicationDate":"2024-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S000399692400133X/pdfft?md5=dcd09c385d87d5850ede0c4443fd3029&pid=1-s2.0-S000399692400133X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141328605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of cariogenic biofilms by using a swept-source optical coherence tomography in vitro 利用体外扫源光学相干断层扫描分析致龋生物膜

IF 3 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2024-05-25 DOI: 10.1016/j.archoralbio.2024.106009

Tomoko Tabata , Hisaichi Nakagawa , Khairul Matin , Masayuki Otsuki , Akira Aoki , Yasunori Sumi , Yasushi Shimada

Objective

The objective was to measure the thickness of Streptococcus mutans (S. mutans) biofilms forming in an oral biofilm reactor (OBR) by using a noninvasive swept-source optical coherence tomography (SS-OCT) system at every 4 h time interval until 20 h and analyze the correlations with the amounts of biofilms.

Methods

S. mutans biofilms were formed on square-shaped bovine enamel blocks inside an OBR. Biofilms were analyzed at every 4 h stage (4 h, 8 h, 12 h, 16 h and 20 h) using a SS-OCT system and a laser scanning confocal microscope (LSCM). The amounts of biofilms were measured at each stage by separating the water insoluble glucan (WIG) and bacterial cells. Co-relationships between the SS-OCT measured biofilm thickness and the amounts of adhered biofilms were analyzed.

Results

The thickness of biofilms detected on SS-OCT images at 4 h stage was 0.059 ± 0.029 (Av ± SD) mm which increased time-dependently in a linear fashion after 8 h stage and reached to 0.435 ± 0.159 mm at 20 h stage and the correlation coefficient was about 0.89. The amounts of biofilms; bacterial optical density (OD) and WIG concentration increased time-dependently were 0.035 ± 0.008 / mm² and 10.328 ± 2.492 µg/ mm² respectively at 20 h stage. Correlation coefficients of 0.66 between ‘the amounts of bacteria’ and ‘biofilm thickness on OCT’ and 0.67 between ‘the amounts of WIG’ and ‘biofilm thickness on OCT’ were obtained, suggesting that there was a relatively positive correlation between them.

Conclusion

The SS-OCT can be a useful tool to measure time-dependent growth of biofilms. Further studies are needed in order to assess biofilms using SS-OCT more accurately.

目的通过使用无创扫源光学相干断层扫描（SS-OCT）系统，测量口腔生物膜反应器（OBR）中形成的变异链球菌（S. mutans）生物膜的厚度，每隔 4 小时测量一次，直至 20 小时，并分析其与生物膜数量的相关性。方法在 OBR 中的方形牛珐琅块上形成变异链球菌生物膜。使用 SS-OCT 系统和激光扫描共聚焦显微镜（LSCM）在每 4 h 阶段（4 h、8 h、12 h、16 h 和 20 h）对生物膜进行分析。通过分离水不溶性葡聚糖（WIG）和细菌细胞，测量每个阶段的生物膜数量。结果 4 h 阶段 SS-OCT 图像上检测到的生物膜厚度为 0.059 ± 0.029（Av ± SD）mm，8 h 阶段后呈线性增长，20 h 阶段达到 0.435 ± 0.159 mm，相关系数约为 0.89。生物膜数量、细菌光密度（OD）和 WIG 浓度的增加与时间无关，在 20 小时阶段分别为 0.035 ± 0.008 / mm2 和 10.328 ± 2.492 µg / mm2。细菌数量 "与 "OCT 上的生物膜厚度 "之间的相关系数为 0.66，"WIG 数量 "与 "OCT 上的生物膜厚度 "之间的相关系数为 0.67，表明它们之间存在相对正相关的关系。为了更准确地使用 SS-OCT 评估生物膜，还需要进一步的研究。

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引用次数: 0

A finite element analysis of periodontal ligament fluid mechanics response to occlusal loading based on hydro-mechanical coupling model 基于流体力学耦合模型的牙周韧带流体力学对咬合负荷响应的有限元分析

IF 3 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2024-05-22 DOI: 10.1016/j.archoralbio.2024.106008

Zhongyu Wang , Sa Du , Huilin Zhu , Ke Yi , Zhihui Tang , Qing Li

Objective

Considering fluid stimulation is one of the essential biomechanical signals for periodontal tissues, this study aims to characterizing fluid mechanics response during occlusal loading by a hydro-mechanical coupling model for periodontal ligament.

Design

Models simulating periodontium with normal bone height and with intraosseous defects were built with three mechanical modules: tooth, periodontal ligament and alveolar bone. Tooth was modeled as linear elastic, and periodontal ligament and alveolar bone as a hydro-mechanical coupling model. Transient analyses under dynamic occlusal loading were performed. Fluid dynamics within periodontal ligament space was simulated and visualized by post-processing module.

Results

Reciprocating oscillatory flow occurred within the periodontal ligament under occlusal loading. Higher pore pressure and fluid velocity were observed in furcation and apical regions compared to mid-root and cervical regions. Intraosseous defects increased pore pressure and fluid velocity within the periodontal ligament, most significantly near the defect.

Conclusion

Based on the results of the hydro-mechanical coupling model, significant oscillatory fluid motion is observed within the periodontal ligament under occlusal loading. Particularly, higher fluid velocity is evident in the furcation and apical areas. Additionally, Intraosseous defects significantly enhance fluid motion within the periodontal ligament.

考虑到流体刺激是牙周组织重要的生物力学信号之一，本研究旨在通过牙周韧带的流体力学耦合模型来描述咬合负荷时的流体力学反应。牙齿为线性弹性模型，牙周韧带和牙槽骨为流体力学耦合模型。在动态咬合负荷下进行了瞬态分析。结果在咬合负荷下，牙周韧带内出现了往复振荡流。与中根和牙颈部相比，在窝沟和根尖区域观察到更高的孔隙压力和流体速度。结论根据水力机械耦合模型的结果，在咬合负荷下牙周韧带内观察到明显的振荡流体运动。特别是在窝沟和根尖区域，流体速度明显更高。此外，骨内缺损也会显著增强牙周韧带内的流体运动。

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引用次数: 0

Locally injected bone marrow-derived mesenchymal stem cells reverts the histopathological changes in the tongue of carbimazole-induced hypothyroidism of male rats 局部注射骨髓间充质干细胞可逆转卡比马唑诱导的甲状腺功能减退雄性大鼠舌头的组织病理学变化

IF 3 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2024-05-22 DOI: 10.1016/j.archoralbio.2024.106010

Hebatallah Mahmoud , Mohamed Badawy , Sahar Abdel-Nasser Mohammed , Maha El Shahawy

Objective

To decipher the role of locally injected bone marrow mesenchymal stem cells (BM-MSCs) in the tongue of hypothyroid rats.

Design

A total 24 male Wister rats were utilized and allocated into 3 groups (n = 8). As for the control group, rats received distilled water via oral gavage. In the hypothyroid group, rats administered carbimazole 5 mg/ 250 g/ day for 6 successive weeks, for hypothyroidism induction. The BM-MSC treated hypothyroid group (BM-MSC group); hypothyroid rats received local injection of 0.5 million BM-MSCs in tongue. Six weeks after BM-MSC injection, tongue samples were processed for Hematoxylin and eosin (H and E) staining, Ki67-immunohistochemistry and histomorphometric analysis.

Results

The hypothyroid group revealed degenerative alterations in the lingual papillae, and apparent thinning of the inferior lingual epithelium compared to their controls. Tongues of the BM-MSC group depicted restoration of the normal tongue histology. The Ki67 immunoreaction was apparently decreased in the lingual epithelium of hypothyroid group compared to their controls, however the BM-MSC group regained Ki67 immunostaining.

Conclusion

Our data suggest that administration of BM-MSCs rescued the degenerative changes in the lingual mucosa and one of the possible underlying mechanisms could be the restoration of cellular proliferation in the lingual epithelium.

目的探讨局部注射骨髓间充质干细胞（BM-MSCs）对甲状腺机能减退大鼠舌头的作用。对照组大鼠口服蒸馏水。甲状腺机能减退组大鼠连续 6 周每天服用卡比马唑 5 毫克/250 克，以诱导甲状腺机能减退。BM-间充质干细胞治疗甲状腺功能减退组（BM-间充质干细胞组）：甲状腺功能减退大鼠舌部局部注射0.5亿个BM-间充质干细胞。结果与对照组相比，甲减组舌乳头发生退行性改变，下舌上皮明显变薄。骨髓间充质干细胞组的舌组织学恢复正常。与对照组相比，甲状腺功能减退组舌上皮的 Ki67 免疫反应明显减弱，但 BM-MSC 组的 Ki67 免疫染色有所恢复。

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引用次数: 0

Oral Candida albicans strain diversity and maintenance in HIV positive women in South Africa 南非艾滋病毒呈阳性妇女口腔白色念珠菌菌株多样性和维持情况

IF 3 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2024-05-18 DOI: 10.1016/j.archoralbio.2024.106007

FJ Owotade , Z. Gulube , M. Patel

Objective

This study investigated C. albicans strain diversity and maintenance in the oral cavity of HIV positive women over a 6 month period.

Study design

C. albicans strains were isolated from 17 HIV positive women at Charlotte Maxeke Academic Hospital, Johannesburg at 3 intervals over a 6 month period. Strains were genotyped using ABC and Multilocus Sequence Typing (MLST) techniques. In the MLST technique, for each strain, a Diploid Sequence Type (DST) number was obtained. Using cluster analysis, an Unweighted Pair Group Method with Arithmetic Mean (UPGMA) dendrogram and a matrix of strain similarities were generated. Strains were also compared to the previous South African isolates documented in the MLST database.

Results

Ninety four percent of women carried the same ABC genotype for 6 months. MLST technique, showed that ten women (58.8%) carried the same DST at 2 visits, while seven (41.2%) carried different DST at all visits. Further analysis showed that 64.7% of women were recolonised with different strains and 35.3% carried the same strains of C. albicans with heterozygosity. A total of 40 diploid sequence types were identified of which 27 DSTs were unique to this study group that were added to the MLST database. Most of the strains were closely related to previously isolated strains from South Africa.

Conclusion

Recolonization of the oral cavity with different strains and microevolution of the original strains of C. albicans can occur, which can be a potential problem for HIV patients, in whom highly virulent and drug resistant strains can emerge.

本研究调查了 6 个月内 HIV 阳性女性口腔中白念珠菌菌株的多样性和维持情况。研究设计在 6 个月内，每隔 3 次从约翰内斯堡 Charlotte Maxeke 学术医院的 17 名 HIV 阳性女性口腔中分离出白念珠菌菌株。使用 ABC 和多焦点序列分型（MLST）技术对菌株进行基因分型。在多焦点序列分型（MLST）技术中，每个菌株都有一个二倍体序列类型（DST）编号。通过聚类分析，生成了非加权配对组算术平均法（UPGMA）树枝图和菌株相似性矩阵。此外，还将菌株与 MLST 数据库中记录的以往南非分离菌株进行了比较。MLST 技术显示，10 名妇女（58.8%）在两次就诊时携带相同的 DST，而 7 名妇女（41.2%）在所有就诊时携带不同的 DST。进一步分析表明，64.7%的妇女再次感染了不同的菌株，35.3%的妇女感染了具有杂合性的相同白僵菌菌株。共鉴定出 40 种二倍体序列类型，其中 27 种 DST 为本研究组所独有，并已添加到 MLST 数据库中。大多数菌株与之前从南非分离的菌株关系密切。结论口腔中可能会出现不同菌株的繁殖以及白僵菌原始菌株的微进化，这对艾滋病患者来说可能是一个潜在的问题，因为在这些患者中可能会出现毒性很强的耐药菌株。

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引用次数: 0

Are deciduous upper molars and lower canines useful for sex estimation? 脱落的上臼齿和下犬齿对性别估计有用吗？

IF 3 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2024-05-17 DOI: 10.1016/j.archoralbio.2024.106006

Cármen Valente , Alexandra Teixeira , Pedro Oliveira , Inês Morais Caldas

Objective

This paper investigates whether deciduous upper molars and lower canines have sexual dimorphic features, exploring these teeth' dimensions and the presence of Zuckerkandl’s tubercle and Carabelli’s cusp on the first and second upper molars.

Design

We analyzed 64 pairs of dental plaster casts from 34 females and 30 males aged between 3 and 12 years. We measured the first and second deciduous upper molars and the lower deciduous canines (maximum mesiodistal and buccolingual length), and we registered the presence of the Zuckerkandl’s tubercle and the Carabelli’s cusp on the first and second upper molars, respectively.

Results

Regarding the differentiation between sexes using Carabelli’s cusp and Zuckerkandl’s tubercle, the classification was not independent of Carabelli’s cusp presence only for tooth 65 (p = 0.035). In all other teeth, whether for Carabelli’s cusp or Zuckerkandl’s tubercle, their presence was similar for both sexes. There were statistically significant differences between sexes (p < 0.05) for the buccolingual measurements of both upper second molars, the first right upper molar, and the right canine. The developed model allowed for a 64.1% accuracy in sex estimation.

Conclusions

The study suggests that while Carabelli’s cusp and Zuckerkandl’s tubercle in upper deciduous molars don't consistently differ between sexes, tooth size, particularly the buccolingual measurements of certain teeth, including upper deciduous molars and lower canines, may provide a more reliable criterion for sex estimation. The developed model depicted moderate accuracy, underscoring the need for a multifactorial approach when estimating sex from skeletal remains. It suggests that while dental features can contribute to sex estimation, they should be used in conjunction with other skeletal or molecular markers to improve accuracy.

目的本文通过研究这些牙齿的尺寸以及第一和第二上臼齿上是否存在Zuckerkandl's结节和Carabelli's尖突，探讨落叶上臼齿和下犬齿是否具有性别二形特征。我们测量了第一和第二上臼齿以及下臼齿犬齿（最大中轴和颊舌侧长度），并分别记录了第一和第二上臼齿上是否存在Zuckerkandl's结节和Carabelli's尖突。结果关于使用卡拉贝里尖突和祖克坎德尔小结区分性别，只有在第 65 颗牙齿上的分类与卡拉贝里尖突的存在无关（p = 0.035）。在所有其他牙齿中，无论是卡拉贝利尖突还是祖克坎德尔小结，其存在对两性来说都是相似的。在上第二臼齿、右上第一臼齿和右犬齿的颊舌侧测量中，男女之间的差异具有统计学意义（p < 0.05）。结论该研究表明，虽然上臼齿的卡拉贝利尖和祖克坎德尔结节在性别上没有一致的差异，但牙齿大小，尤其是某些牙齿（包括上臼齿和下犬齿）的颊舌侧测量值，可能为性别估计提供了更可靠的标准。所开发的模型描述了中等程度的准确性，强调了从骨骼遗骸中估计性别时采用多因素方法的必要性。它表明，虽然牙齿特征有助于性别估计，但应与其他骨骼或分子标记结合使用，以提高准确性。

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引用次数: 0