Archives of oral biology最新文献_第8页

Acidic/abrasive challenges on simulated non-carious cervical lesions development and morphology 酸性/磨蚀性挑战对模拟非龋齿性宫颈病变的发展和形态的影响。

IF 2.2 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2024-10-28 DOI: 10.1016/j.archoralbio.2024.106120

Giovanna C. Denucci , Ian Towle , Cecilia P. Turssi , George J. Eckert , Anderson T. Hara

Objectives

This in vitro investigation assessed how frequency of erosive challenges and duration of toothbrushing abrasion influenced non-carious cervical lesions (NCCLs) development and morphology. Design: Experimental units were prepared using extracted human premolars assigned to four erosive-abrasive frequency protocols (n=16): F0. No acid exposure (negative control), F2.5 K. Acid exposure (1 % citric acid at natural pH) every 2500, F5K. 5000 and F15K. 15000 brushing-strokes. All groups were brushed for 55000 total brushing-strokes. Three-dimension images of the teeth were captured at baseline, after 15000, 35000 and 55000 brushing-strokes, using an intraoral scanner (TRIOS4, 3Shape). WearCompare software (Leeds Digital Dentistry) was used to analyze volumetric tooth loss (mm³) by superimposition followed by subtraction analysis. Lesion angle was measured (ImageJ, NIH) and morphology visually classified. Data were analyzed using ANOVA and Fisher’s Exact tests adopting two-sided 5 % significance level. Results: Tooth loss increased with brushing-strokes overall (p<0.001) and for each erosive-abrasive protocol (p<0.001). Acid exposure significantly increased tooth loss (p<0.001), regardless of brushing interval (p<0.001), however by 35000 strokes no tooth loss difference was observed among acid-exposed groups (p>0.05). Control had significantly sharper mean lesion angle (59°) than all acid-exposed groups (∼145°) (p<0.001), and significantly different lesion shape with 94 % wedge-shaped lesions versus 0 %, respectively (p<0.001). In contrast to the control, acid exposure was associated to more striated lesions. Conclusions: Simulated NCCLs developed and progressed differently and more rapidly in the presence of acidic challenges, regardless of their frequency. Exposure to acid impacted the morphology of lesions.

目的：这项体外研究评估了侵蚀性挑战的频率和刷牙磨损的持续时间如何影响非龋性宫颈病变（NCCL）的发展和形态：这项体外研究评估了侵蚀性挑战的频率和刷牙磨损的持续时间如何影响非龋性牙颈部病变（NCCLs）的发展和形态：设计：使用拔出的人类前臼齿制备实验单位，将其分配到四种侵蚀-磨损频率方案中（n=16）：F0.无酸暴露（阴性对照），F2.5 K.酸暴露（自然 pH 值下的 1 % 柠檬酸），每 2500 次；F5K.5000 和 F15K。15000 次刷洗。所有组的刷牙总次数为 55000 次。使用口内扫描仪（TRIOS4，3Shape）采集基线、15000 次、35000 次和 55000 次刷牙后的牙齿三维图像。使用 WearCompare 软件（利兹数字牙科）通过叠加和减法分析来分析牙齿缺损的体积（mm3）。测量病损角度（ImageJ，NIH）并对形态进行目测分类。数据分析采用方差分析和费雪精确检验，显著性水平为双侧 5%：结果：总体而言，刷牙次数越多，牙齿脱落越严重（P0.05）。对照组的平均病损角度（59°）明显高于所有酸暴露组（∼145°）（p结论：无论酸性挑战的频率如何，模拟 NCCL 在存在酸性挑战的情况下都会以不同的方式快速发展和恶化。接触酸会影响病变的形态。

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引用次数: 0

Effects of quercetin on mineralized dental tissues: A scoping review 槲皮素对矿化牙齿组织的影响：范围综述

IF 2.2 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2024-10-24 DOI: 10.1016/j.archoralbio.2024.106119

Gabriel Pereira Nunes , Renata de Oliveira Alves , Matheus Henrique Faccioli Ragghianti , Alexandre Henrique dos Reis-Prado , Priscila Toninatto Alves de Toledo , Tamires Passadori Martins , Ana Paula Miranda Vieira , Geórgia Rondó Peres , Cristiane Duque

Objective

This scoping review (SR) aimed to investigate the impact of quercetin on mineralized dental tissues intended to be used in preventive and restorative dentistry.

Methods

This SR was conducted following the PRISMA-ScR statement. A comprehensive search was performed across databases for articles published up to March 2024. Eligible studies included in vitro and in situ studies and evaluating the potential therapeutic effects of quercetin on dental enamel and dentin. Data were extracted, and synthesis of study findings was conducted.

Results

Out of the 2322 records screened, 22 studies were included in the review. Quercetin, in solution or into dental materials increased the bond strength to enamel and dentin. Additionally, quercetin also enhanced the bond strength of enamel after bleaching. Co-administration of quercetin with fluoride prevented erosive wear and inhibited the proteolytic activity in dentin more effectively than either agent alone. Hardness and modulus of elasticity was higher in dentin treated with quercetin compared to placebo. Reduction of nanoleakage at the composite-dentin interface was reduced in the presence of quercetin as a solution or incorporated into dental adhesives.

Conclusions

Quercetin exhibits promising therapeutic effects on mineralized dental tissues, including remineralization and enhancement of bond strength. It shows potential as a multifunctional agent for improving the longevity and effectiveness of dental biomaterials, as well as in preventing erosion and dental caries. However, as these conclusions are largely drawn from lab-based (in vitro) studies, further research, including clinical trials, is needed to fully explore its therapeutic potential and applications in dentistry.

目的本范围综述（SR）旨在研究槲皮素对拟用于预防和修复牙科的矿化牙组织的影响。在各数据库中全面检索了截至 2024 年 3 月发表的文章。符合条件的研究包括体外和原位研究，以及评估槲皮素对牙釉质和牙本质潜在治疗效果的研究。在筛选出的 2322 条记录中，有 22 项研究被纳入综述。在溶液中或牙科材料中加入槲皮素可增加与牙釉质和牙本质的粘合强度。此外，槲皮素还能增强漂白后珐琅质的粘合强度。与单独使用其中一种药剂相比，同时使用槲皮素和氟化物能更有效地防止侵蚀性磨损和抑制牙本质中的蛋白水解活性。与安慰剂相比，使用槲皮素的牙本质硬度和弹性模量更高。结论槲皮素对矿化牙组织具有良好的治疗效果，包括再矿化和增强粘接强度。槲皮素作为一种多功能制剂，在提高牙科生物材料的寿命和有效性以及预防侵蚀和龋齿方面具有潜力。不过，由于这些结论主要来自实验室（体外）研究，因此还需要进一步的研究，包括临床试验，以充分探索其在牙科中的治疗潜力和应用。

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引用次数: 0

Allyl isothiocyanate suppressed periodontal tissue destruction in mice via bacteriostatic and anti-inflammatory activities against Porphyromonas gingivalis 异硫氰酸烯丙酯通过对牙龈卟啉单胞菌的抑菌和消炎作用抑制小鼠牙周组织的破坏

IF 2.2 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2024-10-23 DOI: 10.1016/j.archoralbio.2024.106118

Yukako Minato, Yukari Aoki-Nonaka, Hnin Yu Lwin, Daiki Ando, Yuko Warita, Aoi Matsugishi-Nasu, Takumi Hiyoshi, Naoki Takahashi, Koichi Tabeta

Objectives

Allyl isothiocyanate (AITC) is a phytochemical that is abundantly present in cruciferous vegetables, such as wasabi and mustard. Among its pharmacological properties, it demonstrates anticancer, antifungal, and anti-inflammatory activities. This study aimed to investigate the functions of AITC against periodontopathic bacteria and its effects on a mouse model of periodontitis.

Design

The antimicrobial and antibiofilm functions of AITC were assessed against Porphyromonas gingivalis, Fusobacterium nucleatum, and Streptococcus mitis. To clarify its anti-inflammatory effects, macrophage-like cells from THP-1 were stimulated with P. gingivalis lipopolysaccharide (LPS), and the release of inflammatory cytokines was analyzed by ELISA. Experimental periodontitis was induced in 9-week-old mice by ligation and oral infection of P. gingivalis, and AITC was injected into the gingiva once daily for 8 days. Alveolar bone resorption was evaluated by measuring the exposed root area. Gene expressions in the periodontal tissue were analyzed via qPCR.

Results

AITC exerted weak bacteriostatic effects against P. gingivalis, inhibiting biofilm formation. AITC also impeded the production of interleukin-6 and tumor necrosis factor-α induced by P. gingivalis LPS. Additionally, transient receptor potential ankyrin 1(TRPA1) channel agonist inhibited the anti-inflammatory effects of AITC. In vivo, AITC inhibited alveolar bone destruction and decreased the gene transcription of Il6 in the periodontal tissue.

Conclusion

AITC exerted weak bacteriostatic and anti-inflammatory effects against P. gingivalis, reducing alveolar bone destruction and suppressing the inflammatory response in experimental periodontitis. Therefore, AITC may serve as a valuable adjunct in controlling periodontal disease.

目的异硫氰酸烯丙酯（AITC）是一种植物化学物质，大量存在于芥末和芥菜等十字花科蔬菜中。在其药理特性中，它具有抗癌、抗真菌和抗炎活性。本研究旨在探讨 AITC 对抗牙周病细菌的功能及其对牙周炎小鼠模型的影响。设计评估了 AITC 对抗牙龈卟啉单胞菌、核酸化脓杆菌和米炎链球菌的抗菌和抗生物膜功能。为明确其抗炎作用，用牙龈卟啉菌脂多糖（LPS）刺激 THP-1 巨噬细胞样细胞，并用 ELISA 分析炎症细胞因子的释放。通过结扎和口腔感染牙龈脓毒性噬菌体诱导 9 周大的小鼠患上实验性牙周炎，然后向牙龈注射 AITC，每天一次，连续注射 8 天。通过测量暴露的牙根面积来评估牙槽骨吸收情况。结果 AITC 对牙龈脓毒性龈脓杆菌有微弱的抑菌作用，可抑制生物膜的形成。AITC 还能抑制牙龈脓疱梭菌 LPS 诱导的白细胞介素-6 和肿瘤坏死因子-α 的产生。此外，瞬时受体电位ankyrin 1（TRPA1）通道激动剂抑制了 AITC 的抗炎作用。结论 AITC 对牙龈脓毒性龈脓杆菌有微弱的抑菌和抗炎作用，能减少牙槽骨破坏，抑制实验性牙周炎的炎症反应。因此，AITC 可作为控制牙周疾病的一种有价值的辅助药物。

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引用次数: 0

How malocclusion interferes with tissue inhibitor of metalloproteinase-1 expression and morphology of the articular cartilage of the mandible in female rats 错牙合畸形如何影响雌性大鼠下颌骨关节软骨中金属蛋白酶组织抑制剂-1的表达和形态

IF 2.2 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2024-10-21 DOI: 10.1016/j.archoralbio.2024.106117

Carolina Brioschi Mathias , Rebeca Ferreira Badaró , Willian Grassi Bautz , Leticia Nogueira da Gama-de-Souza

Objective

The purpose of this study was to investigate morphological alterations and tissue inhibitor of metalloproteinase-1 expression in the articular cartilage of the mandible under conditions of experimentally induced malocclusion.

Design

Twenty-four 8-week-old female Wistar rats were used and divided into control and experimental groups with two different treatment periods (2 and 4 weeks). Sagittal malocclusions were orthodontically created, causing mesial movement of the first molars and distalization of the third molars unilaterally and on opposite sides of the arches. Sagittal sections of the articular cartilage of the mandible were subjected to hematoxylin and eosin and immunohistochemistry for tissue inhibitor of metalloproteinase-1. Chi-square and MannWhitney U tests were applied.

Results

Animals treated for 2 and 4 weeks showed morphological alterations in articular cartilage of the mandible. The main findings were thickening of the posterior third, layer derangement, osteoclast activity and osteophyte formation. Among the cellular aspects, the presence of chondrocytes with condensed nuclei and cytoplasm reduction were observed. The enzyme in control animals was observed only in the mature layer. Treated animals showed immunopositive cells in the proliferative and mature layers, and in the 2-week treated group, the posterior third of the cartilage had more immunolabeled cells than control (P=0.0291).

Conclusions

The occlusal disorder caused morphological changes in articular cartilage of the mandible, probably due to the attempt to adapt to the new condition. Tissue inhibitor of metalloproteinase-1 expression may play a role as an initial modulator in the biological events observed in articular cartilage of the mandible.

设计采用24只8周大的雌性Wistar大鼠，分为对照组和实验组，治疗周期分别为2周和4周。通过正畸造成矢状错颌，使第一磨牙向中侧移动，第三磨牙向远侧移动，单侧且位于牙弓的两侧。对下颌骨关节软骨的矢状切片进行苏木精、伊红和组织金属蛋白酶抑制剂-1的免疫组化。结果治疗 2 周和 4 周的动物下颌骨关节软骨发生了形态学改变。主要表现为后三分之一增厚、层错位、破骨细胞活性和骨赘形成。在细胞方面，观察到软骨细胞的细胞核浓缩，细胞质减少。对照组动物仅在成熟层中观察到酶。结论咬合紊乱导致下颌骨关节软骨发生形态学变化，可能是由于试图适应新的条件。组织金属蛋白酶抑制剂-1的表达可能在下颌骨关节软骨中观察到的生物事件中起到了初始调节剂的作用。

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引用次数: 0

The important role of the Wnt/β-catenin signaling pathway in small molecules mediated gingival mesenchymal stem cells transdifferentiate into neuron-like cells Wnt/β-catenin信号通路在小分子介导的牙龈间充质干细胞向神经元样细胞转分化过程中的重要作用。

IF 2.2 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2024-10-21 DOI: 10.1016/j.archoralbio.2024.106115

Qiuying Liang , Chuhan Zhang , Peiyi Lv , Yongmao Huang , Hang Zhao , Shan Jiang , Wenan Xu

Objective

Given their neural crest origin, gingival mesenchymal stem cells (GMSCs) possess high neurogenic potential, which makes them suitable for cell replacement therapy against neurodegenerative diseases. This study investigated whether GMSCs can be transdifferentiated into neurons in vitro using a protocol involving small molecules VCRFY (VPA, CHIR99021, Repsox, Forskolin, and Y-27632). The regulatory mechanisms of key signaling pathways were also investigated.

Methods

Neuronal induction of GMSCs was conducted using a small molecules-based protocol over 7 days, which included the evaluation of cell morphology, proliferation, expressions of neurogenic markers, and intracellular calcium oscillation. The activation of canonical the Wnt signaling pathway was assessed by examining the protein content and subcellular localization of β-catenin.

Results

Small molecules-treated GMSCs displayed neuronal morphology and increased expression of neurogenic markers, including class III beta-tubulin (TUJ1), neuron-specific enolase (NSE), microtube-associated protein 2 (MAP2), and neurofilament medium (NFM), verified through RT-qPCR, western blotting, and immunocytochemistry. Based on the results of Fluo-4 AM calcium flux assay, small molecules-treated GMSCs exhibited enhanced electrophysiological activity. GMSC proliferation halted after 2 days of treatment. Among the small molecules, CHIR99021 exhibited the highest neuronal induction efficiency. Furthermore, activation of the Wnt/β-catenin signaling pathway augmented neuronal differentiation.

Conclusions

Small molecule-based cellular reprogramming can efficiently generate neurons from GMSCs, with Wnt/β-catenin signaling to play a critical role in neuronal induction.

目的：牙龈间充质干细胞（GMSCs）起源于神经嵴，具有很高的神经源潜能，因此适合用于神经退行性疾病的细胞替代疗法。本研究通过使用小分子 VCRFY（VPA、CHIR99021、Repsox、Forskolin 和 Y-27632），研究了牙龈间充质干细胞能否在体外转分化为神经元。此外，还对关键信号通路的调控机制进行了研究：采用基于小分子的方案对 GMSCs 进行了为期 7 天的神经元诱导，包括评估细胞形态、增殖、神经原标志物的表达和细胞内钙振荡。通过检测β-catenin的蛋白含量和亚细胞定位，评估了Wnt信号通路的激活情况：结果：通过RT-qPCR、Western印迹和免疫细胞化学证实，小分子处理的GMSCs显示了神经元形态和神经原标志物表达的增加，包括III类β-tubulin（TUJ1）、神经元特异性烯醇化酶（NSE）、微管相关蛋白2（MAP2）和神经丝中（NFM）。根据 Fluo-4 AM 钙通量测定的结果，小分子处理的 GMSCs 表现出更强的电生理活性。处理 2 天后，GMSC 增殖停止。在这些小分子中，CHIR99021 的神经元诱导效率最高。此外，激活 Wnt/β-catenin 信号通路可促进神经元分化：结论：基于小分子的细胞重编程可有效地从GMSCs产生神经元，其中Wnt/β-catenin信号在神经元诱导中起着关键作用。

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引用次数: 0

Evaluation of salivary melatonin and MMP-9 levels in periodontal diseases 评估牙周病患者唾液中的褪黑激素和 MMP-9 水平。

IF 2.2 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2024-10-20 DOI: 10.1016/j.archoralbio.2024.106116

Ali Batuhan Bayırlı , Ceyda Gürhan , Ercan Saruhan

Objective

The aim of this study was to evaluate salivary matrix metalloproteinase-9 (MMP-9) and melatonin levels in individuals with periodontal health, gingivitis, and periodontitis.

Design

A total of 170 participants were enrolled in this study. They included 57 periodontally healthy individuals, 58 gingivitis patients, and 55 periodontitis patients. Saliva samples were collected by passive drool technique. The levels of MMP-9 and melatonin in saliva were measured biochemically using the ELISA method.

Results

Salivary MMP-9 levels in the periodontitis group were significantly higher than those in the gingivitis and periodontally healthy groups, while salivary melatonin levels were significantly lower (p<0.001). A positive correlation was observed between clinical periodontal parameters and salivary MMP-9 levels, while salivary melatonin levels were negatively correlated (p<0.001). A negative correlation was also observed between salivary MMP-9 levels and salivary melatonin levels (p<0.001).

Conclusion

This study shows that the level of melatonin in saliva is associated with periodontal disease and with the level of MMP-9 in saliva, which plays a role in this disease.

研究目的本研究旨在评估牙周健康、牙龈炎和牙周炎患者的唾液基质金属蛋白酶-9（MMP-9）和褪黑激素水平：设计：本研究共招募了 170 名参与者。设计：这项研究共招募了 170 名参与者，其中包括 57 名牙周健康者、58 名牙龈炎患者和 55 名牙周炎患者。采用被动流口水技术采集唾液样本。采用酶联免疫吸附法对唾液中的 MMP-9 和褪黑激素水平进行生化测定：结果：牙周炎组唾液中 MMP-9 的水平明显高于牙龈炎组和牙周健康组，而唾液中褪黑激素的水平则明显低于牙龈炎组和牙周健康组（p 结论：该研究表明，褪黑激素在牙周炎患者唾液中的水平明显高于牙龈炎组和牙周健康组：本研究表明，唾液中褪黑激素的水平与牙周病和唾液中 MMP-9 的水平有关，而 MMP-9 在牙周病中起着一定的作用。

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引用次数: 0

Mimicking and in vitro validating chronic inflammation in human gingival fibroblasts 模拟和体外验证人类牙龈成纤维细胞的慢性炎症。

IF 2.2 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2024-10-19 DOI: 10.1016/j.archoralbio.2024.106113

Anne Eriksson Agger , Athina Samara , Tianxiang Geng , Ole Kristoffer Olstad , Janne Elin Reseland

Objective

The aim of this study was to identify and validate in vitro conditions that may mimic the translational, cytokine and chemokine profiles observed in human inflamed gingiva in vivo.

Design

Primary human gingiva fibroblast cells (HFIB-G) were cultured under serum starvation conditions (0 – 10 %), supplemented with increasing lipopolysaccharide (LPS) concentrations (0.1, 1, or 10 µg/ml) from two bacterial strains E. coli and P. gingivalis and 0.1, 1, or 10 ng/ml recombinant interleukin 1β (IL-1β), alone or in combinations. The levels of cytokines/chemokines were measured in the cell culture medium by Luminex, and gene expression was quantified by Affymetrix microarrays at 24, 48 and 72 h.

Results

Inflammation markers were not elevated after stimulation with P. gingivalis LPS, while E. coli LPS and IL-1β individually increased the secretion of interleukin 6 (IL-6) and monocyte chemoattractant protein-1 (MCP-1) to the cell culture medium. IL-1β administration also increased the secretion of several factors, including tumor necrosis factor (TNFα). However, the combination of 1 µg/ml E. coli LPS, 1 ng/ml IL-1β and serum starvation led to increased secretion of IL-6, TNFα, in addition to other factors found in inflamed tissue. Gene expression analyses revealed that this combination not only enhanced the expression interleukins/chemokines genes but also T helper cell signaling and matrix metalloproteinases.

Conclusion

Serum reduction in cell culture medium together with the administration of E. coli LPS and IL-1β resulted in gene expression and secreted cytokine/chemokine profiles similar to that found in vivo during chronic inflammation.

研究目的本研究旨在确定和验证体外条件，以模拟在体内发炎的人类牙龈中观察到的翻译、细胞因子和趋化因子特征：原代人牙龈成纤维细胞（HFIB-G）在血清饥饿条件（0 - 10 %）下培养，同时补充浓度不断升高的脂多糖（LPS）（0.1、1 或 10 µg/ml），脂多糖来自两种细菌菌株大肠杆菌和牙龈脓杆菌，重组白细胞介素 1β (IL-1β)浓度分别为 0.1、1 或 10 ng/ml，可单独使用或混合使用。用 Luminex 测定细胞培养液中细胞因子/凝血因子的水平，并在 24、48 和 72 小时后用 Affymetrix 芯片对基因表达进行量化：结果：用牙龈脓毒性球菌 LPS 刺激后，炎症标志物没有升高，而大肠杆菌 LPS 和 IL-1β 则分别增加了细胞培养基中白细胞介素 6（IL-6）和单核细胞趋化蛋白-1（MCP-1）的分泌。IL-1β 还能增加多种因子的分泌，包括肿瘤坏死因子（TNFα）。然而，将 1 µg/ml 大肠杆菌 LPS、1 ng/ml IL-1β 和血清饥饿结合使用会导致 IL-6、TNFα 以及炎症组织中的其他因子分泌增加。基因表达分析表明，这种组合不仅增强了白细胞介素/趋化因子基因的表达，还增强了 T 辅助细胞信号传导和基质金属蛋白酶的表达：结论：减少细胞培养基中的血清含量，同时给予大肠杆菌 LPS 和 IL-1β 会导致基因表达和分泌的细胞因子/趋化因子谱与体内慢性炎症时的情况相似。

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引用次数: 0

Impact of botulinum toxin type A on tooth movement and bone remodeling in male Wistar rats A 型肉毒毒素对雄性 Wistar 大鼠牙齿移动和骨骼重塑的影响

IF 2.2 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2024-10-18 DOI: 10.1016/j.archoralbio.2024.106105

Joana Estephany Gordillo Yépez , Renata Machado Marangon , Aline Cristina Batista Rodrigues Johann , Bruno Massa de Viveiros , Patricia Kern Di Scala Andreis , Luana Vosgerau , Sara Moreira Leal Salvação , Orlando Motohiro Tanaka , Odilon Guariza-Filho , Sergio Aparecido Ignácio , Elisa Souza Camargo

Objective

We evaluated whether the use of botulinum neurotoxin type A (BTX-A) in masticatory muscles influences tooth movement and bone remodeling.

Design

Seventy-seven male Wistar rats were allocated to the groups: S - Saline (n=20); SM - Saline with movement (n=20); BT - Botulinum toxin (n=18); BTM - Botulinum toxin with movement (n=19). On day 1, 0.02 mL of sterile 0.9 % saline was administered to groups S and SM and BTX-A (1 U in 0.02 mL of saline) to groups BT and BTM, in the masseter and temporal muscles laterally. On day 30, a nickel titanium spring was installed to move the first maxillary molar and euthanasia was performed on days 32 and 51. Tooth displacement, maxillary and mandibular bone volumes, collagen neoformation, bone and root resorptions, and masseter morphometry were assessed. Statistical analysis was conducted (p < 0.05).

Results

A higher percentage of type I collagen was observed in the BT than in the S group on day 51 and lower mass, length, and diameter of the masseter fibers in BT and BTM (p < 0.05). Tooth displacement, bone volume, bone and root resorptions, hyaline area, and masseter height showed no difference among groups with and without BTX-A, regardless of tooth movement (p > 0.05).

Conclusions

BTX-A did not interfere with tooth displacement, bone volume, and dental and periodontal tissues related to tooth movement in rats; it increased mature collagen in animals without tooth movement; and it caused a decrease in the mass, length, and diameter of the masseter fibers.

目的我们评估了在咀嚼肌中使用A型肉毒杆菌神经毒素（BTX-A）是否会影响牙齿移动和骨重塑：将 77 只雄性 Wistar 大鼠分配到不同组别：S -生理盐水组（n=20）；SM -含运动的生理盐水组（n=20）；BT -肉毒杆菌毒素组（n=18）；BTM -含运动的肉毒杆菌毒素组（n=19）。第 1 天，给 S 组和 SM 组注射 0.02 mL 0.9 % 无菌生理盐水，给 BT 组和 BTM 组注射 BTX-A（1 U 加入 0.02 mL 生理盐水中），注射部位为咀嚼肌和颞侧肌肉。第 30 天，安装镍钛弹簧以移动上颌第一磨牙，第 32 天和第 51 天实施安乐术。对牙齿移位、上颌骨和下颌骨体积、胶原新生、骨和牙根吸收以及咀嚼肌形态进行了评估。进行了统计分析（P < 0.05）：结果：第 51 天，观察到 BT 组 I 型胶原的比例高于 S 组，BT 组和 BTM 组的颌间肌纤维的质量、长度和直径均低于 S 组（P < 0.05）。牙齿移位、骨量、骨和牙根吸收、透明区和咀嚼肌高度在使用 BTX-A 和未使用 BTX-A 的组间无差异，与牙齿移动无关（P > 0.05）：结论：BTX-A 不影响大鼠牙齿移位、骨量以及与牙齿移动相关的牙齿和牙周组织；它增加了无牙齿移动动物的成熟胶原蛋白；它导致颌间肌纤维的质量、长度和直径下降。

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引用次数: 0

Evaluation of hard tissue characteristics and calcifications in pulp tissue of hypomineralized permanent molars using micro-computed tomography 使用微型计算机断层扫描评估低矿化恒磨牙牙髓组织中的硬组织特征和钙化。

IF 2.2 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2024-10-18 DOI: 10.1016/j.archoralbio.2024.106111

Didem Sakaryalı Uyar , Hazal Karslıoğlu , Mert Ocak , Hakan Hamdi Çelik

Objectives

To determine and compare pulp volume, dentin mineral density, presence of microcracks, pulp stones, and accessory canals, as well as their localizations in root regions for hypomineralized and healthy teeth.

Design

This study included 60 extracted permanent molar teeth, categorized into hypomineralized and healthy groups (n = 30 each). The hypomineralized group comprised molar teeth with limited white, yellow, or brown opacities, post-eruptive breakdown, or extensive restoration or crown damage. The healthy group included caries-free molar teeth without these characteristics. Using 3D micro-computed tomography images pulp volume, dentin mineral density, and the presence and locations of microcracks, pulp stones, and accessory canals were determined for each group. Statistical analyses were conducted using Independent T-test and Chi-square test, with significance set at p < 0.05.

Results

There was no statistically significant difference between the groups regarding pulp volume and microcracks (p ≥ 0.05). The number of accessory canals was significantly greater in the cervical (p = 0.011; p < 0.05) and middle (p = 0.010; p < 0.05) regions of the hypomineralized teeth than healthy teeth. Dentin mineral density was statistically higher in the apical, middle, and cervical root regions (p < 0.001; p < 0.05); however, the number of pulp stones was found to be greater in the cervical regions of healthy teeth compared with those with hypomineralization (p = 0.026; p < 0.05).

Conclusion

There were lower dentin mineral density measurements, a decreased number of pulp stones in the cervical region, and a greater number of accessory canals in the middle and cervical regions of hypomineralized teeth compared with healthy teeth.

目的确定并比较低矿化和健康牙齿的牙髓体积、牙本质矿物质密度、是否存在微裂缝、牙髓结石和附属管，以及它们在牙根区域的位置：本研究包括 60 颗拔出的恒磨牙，分为低矿化组和健康组（各 30 颗）。低矿化组包括有局限性白斑、黄斑或棕斑、萌出后破损或大面积修复体或牙冠损坏的磨牙。健康组包括没有这些特征的无龋磨牙。使用三维微型计算机断层扫描图像确定每组的牙髓体积、牙本质矿物质密度以及微裂缝、牙髓结石和附属管的存在和位置。统计分析采用独立 T 检验和卡方检验，显著性以 p < 0.05 为限：结果：在牙髓体积和微裂缝方面，组间差异无统计学意义（P≥0.05）。低矿化牙齿的牙颈部（p = 0.011；p < 0.05）和中部（p = 0.010；p < 0.05）的附属根管数量明显多于健康牙齿。据统计，根尖、根中部和根颈部的牙本质矿物质密度较高（p < 0.001；p < 0.05）；然而，与矿化不足的牙齿相比，健康牙齿根颈部的牙髓结石数量较多（p = 0.026；p < 0.05）：结论：与健康牙齿相比，低矿化牙齿的牙本质矿物质密度测量值较低，牙颈部的牙髓结石数量较少，中间和牙颈部的附属管数量较多。

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引用次数: 0

Analysis of dental chipping for identifying and diagnosing tooth fracture patterns in osteological series 在骨学系列中分析牙齿缺损以识别和诊断牙齿断裂模式。

IF 2.2 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2024-10-18 DOI: 10.1016/j.archoralbio.2024.106114

Á. Rubio Salvador , S.A. Jiménez-Brobeil , M. Lozano

Objective

To develop a specific methodology for identifying dental chipping and determining its temporal occurrence in past populations.

Design

The analysed sample comprised of 2191 human teeth from various Bronze Age on the Iberian Peninsula (Argar culture, 1900–1450 cal BC). Among these, 471 chipped teeth were identified. Chipping was examined using various microscopic techniques (digital three-dimensional, optical, and confocal), focusing on distribution, morphology, position in the tooth, extent of damage, and post-chipping antemortem modifications (PCAM).

Results

The distribution and morphology of the chips enabled the identification chipping mechanism of the chipping, providing valid criteria to distinguish between antemortem and postmortem chipping. Microscopic analyses of the chipping segments—edges, sidewalls, surface, and surrounding area—facilitated determination of the time the chip ocurred (antemortem: recent, less recent, or not recent).

Conclusions

While experimental studies provide valuable insights into chipping mechanisms, many criteria may not be applicable to past populations because of the presence of PCAM. The lack of PCAM in some Argaric teeth suggests that previous studies may have underestimated the prevalence of chipping in past populations.

目的：制定识别牙齿缺损的具体方法，并确定其在过去人群中出现的时间：设计：分析的样本包括来自伊比利亚半岛不同青铜时代（阿尔加文化，公元前 1900-1450 年）的 2191 颗人类牙齿。在这些牙齿中，有 471 颗牙齿有缺口。我们使用各种显微技术（数字三维、光学和共聚焦）对崩裂进行了检查，重点是崩裂的分布、形态、在牙齿中的位置、损坏程度以及崩裂后的死前改造（PCAM）：碎屑的分布和形态有助于确定碎屑的碎裂机制，为区分死前碎屑和死后碎屑提供了有效的标准。对崩裂片段--边缘、侧壁、表面和周围区域--的显微分析有助于确定崩裂发生的时间（死前：近期、较近期或非近期）：尽管实验研究为了解崩解机制提供了宝贵的资料，但由于 PCAM 的存在，许多标准可能并不适用于过去的人群。一些阿尔加里克人的牙齿中缺乏 PCAM，这表明以前的研究可能低估了崩蚀在过去人群中的发生率。

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引用次数: 0