Archives of oral biology最新文献_第8页

Combined bioinformatics and experimental validation identifies m1A-modified genes as potential diagnostic biomarkers in periodontitis and diabetes 结合生物信息学和实验验证确定m1a修饰基因作为牙周炎和糖尿病的潜在诊断生物标志物。

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2025-10-14 DOI: 10.1016/j.archoralbio.2025.106428

Kai Wang , Aiguo Wang , Liewang Qiu , Ling Xu , Jingjing Wei

Objective

To identify m1A-modified genes with diagnostic potential linking periodontitis and type 2 diabetes mellitus (T2DM) by integrating bioinformatics and experimental validation.

Design

Transcriptomic data for periodontitis and T2DM patients were integrated from the GEO database to analyze m1A-related gene expression. A diagnostic model was constructed using ridge and logistic regression. Gene function enrichment, immune infiltration, and protein-protein interaction analyses explored m1A regulatory mechanisms based on m1A scoring and patient clustering models. Gingival tissue samples were collected from periodontitis patients and healthy controls, and a streptozotocin-induced diabetic β-cell model was established. qRT-PCR was performed to validate candidate genes (RRP8, ALKBH3, MAK16, and DDX18). Statistical comparisons were conducted using the non-parametric Mann–Whitney U test.

Results

Several m1A-related genes were differentially expressed in both periodontitis and T2DM. RRP8 and ALKBH3 had high predictive value, with area under the curve (AUC) values of 0.80 (periodontitis) and 0.72 (T2DM). m1A scoring and patient clustering models effectively distinguished patient groups with distinct transcriptomic and immune profiles. Hub genes MAK16 and DDX18 showed consistent expression patterns and strong correlations with immune infiltration. qRT-PCR confirmed significant downregulation of RRP8, ALKBH3, MAK16, and DDX18 in inflamed gingival tissues, and upregulation in the diabetic cell model (p < 0.05), supporting the bioinformatics findings.

Conclusions

This integrative study identifies key m1A-modified genes potentially linking periodontitis and diabetes. The combination of bioinformatics analysis and experimental validation highlights their potential as diagnostic biomarkers and provides novel insights into shared molecular mechanisms of these comorbid conditions.

目的：通过生物信息学和实验验证相结合的方法，鉴定具有牙周炎与2型糖尿病（T2DM）诊断潜力的m1a修饰基因。设计：从GEO数据库中整合牙周炎和T2DM患者的转录组学数据，分析m1a相关基因表达。采用岭回归和逻辑回归建立诊断模型。基于m1A评分和患者聚类模型，基因功能富集、免疫浸润和蛋白-蛋白相互作用分析探索了m1A的调控机制。收集牙周炎患者和健康对照者的牙龈组织标本，建立链脲佐菌素诱导的糖尿病β细胞模型。采用qRT-PCR验证候选基因（RRP8、ALKBH3、MAK16和DDX18）。采用非参数Mann-Whitney U检验进行统计比较。结果：几个m1a相关基因在牙周炎和T2DM中均有差异表达。RRP8和ALKBH3具有较高的预测价值，曲线下面积（AUC）值分别为0.80（牙周炎）和0.72 （T2DM）。m1A评分和患者聚类模型有效区分具有不同转录组和免疫谱的患者组。枢纽基因MAK16和DDX18表达模式一致，且与免疫浸润有很强的相关性。qRT-PCR证实了炎症牙龈组织中RRP8、ALKBH3、MAK16和DDX18的显著下调，以及糖尿病细胞模型中的上调（p ）。结论：这项综合研究鉴定了关键的ma1修饰基因，可能与牙周炎和糖尿病有关。生物信息学分析和实验验证的结合突出了它们作为诊断性生物标志物的潜力，并为这些共病的共同分子机制提供了新的见解。

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引用次数: 0

Fucose as a biomarker in oral potentially malignant disorders and oral squamous cell carcinoma: A systematic review and meta-analysis focus作为口腔潜在恶性疾病和口腔鳞状细胞癌的生物标志物：系统综述和荟萃分析。

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2025-10-14 DOI: 10.1016/j.archoralbio.2025.106423

Shruti Gupta , Deepti Sharma , Anita Hooda , Mala Kamboj

Objectives

This systematic review and meta-analysis aimed to investigate the potential of fucose as a biomarker for oral potentially malignant disorders (OPMDs) and oral squamous cell carcinoma (OSCC).

Design

Relevant articles were searched across multiple databases, including PubMed, Scopus, Google Scholar, Science Direct, and Clinical Key. Twenty-three studies that met the eligibility criteria were included for qualitative review; out of which, 20 studies that provided data for comparison between study and control groups were further subjected to meta-analysis.

Results

The analysis revealed significantly high fucose levels in the saliva of OPMD patients (p = 0.013) and OSCC patients (p = 0.003) compared to healthy controls. Additionally, fucose levels were elevated in the serum of patients with OSCC (p < 0.001) and oral leukoplakia (p = 0.027) compared to controls.

Conclusion

The findings of this meta-analysis suggest that fucose could serve as a potential biomarker for OPMDs and OSCC. However, the presence of significant heterogeneity in serum evaluation and the limited studies focusing on saliva indicate the need for further investigation.

目的：本系统综述和荟萃分析旨在探讨病灶作为口腔潜在恶性疾病（OPMDs）和口腔鳞状细胞癌（OSCC）生物标志物的潜力。设计：在多个数据库中检索相关文章，包括PubMed、Scopus、谷歌Scholar、Science Direct和Clinical Key。23项符合资格标准的研究被纳入定性评价；其中，有20项研究提供了实验组与对照组比较的数据，进一步进行meta分析。结果：与健康对照组相比，OPMD患者（p = 0.013）和OSCC患者（p = 0.003）唾液中的病灶水平显著升高。此外，OSCC患者血清中的病灶水平升高（p ）。结论：本荟萃分析结果表明，病灶可作为opmd和OSCC的潜在生物标志物。然而，在血清评估中存在显著的异质性和有限的研究集中在唾液表明需要进一步的研究。

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引用次数: 0

Optimization and evaluation of antibacterial extracts from Acacia nilotica, Psidium guajava, Syzygium aromaticum, and Salvadora persica to inhibit dental caries 金合欢、番石榴、香薷、桃金娘抑菌提取物抑龋效果的优化与评价。

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2025-10-14 DOI: 10.1016/j.archoralbio.2025.106426

Chamundeswari Kandasamy , Arunadevi Baladhandapani , Renuka Devi Ponnuswamy , Hemananthan Eswaran , Vinodhini Karthikeyan , Saranya Rajendran

Objective

Dental caries is a significant global issue, which affects approximately 44 % of the global population. The objective of this research is to extract crude phytochemicals from Psidium guajava, Acacia nilotica, Syzygium aromaticum, and Salvadora persica and also optimizing using RSM. Therefore, this aims to maximize the antibacterial potential of the extracts at effective inhibition of dental caries

Method

The crude extracts from the plants samples, with varying extraction parameters, such as ethanol concentrations, temperatures, and solid-solvent ratios was done using Soxhlet method and antibacterial activity was determined through well and disc diffusion methods along with optimization was performed by Response Surface Methodology (RSM).

Results

The results depict that Psidium guajava exhibited the strongest antibacterial activity against S. mutans and Lactobacillus, with inhibition zones of 36 mm & 20 mm and 32 mm & 24 mm for both well and disc diffusion. Similarly, Syzygium aromaticum also shows slight stronger inhibition zones, Acacia nilotica exhibited moderate activity and in contrast, Salvadora persica showed limited activity. RSM optimization was validated and with well fitted quadratic models R² values ranging from 0.69 to 0.79. Predicted and experimental values are in close agreement between with differences below 0.5 %.

Conclusion

These findings suggest that P. guajava, S. aromaticum and A. nilotica have strong potential as natural antibacterial agents in oral care formulations.

目的：龋齿是一个重要的全球性问题，影响了全球约44% %的人口。本研究的目的是提取番石榴、金合欢、香合木和木耳的粗化学成分，并对其进行RSM优化。方法：采用索氏法提取不同提取参数（乙醇浓度、温度、固液比）的植物粗提物，采用孔法测定其抑菌活性，并采用响应面法（RSM）进行优化。结果：瓜石榴对变形链球菌和乳酸菌的抑菌活性最强，孔扩散区和盘扩散区分别为36 mm和20 mm和32 mm和24 mm。同样，Syzygium aromaticum也表现出稍强的抑制区，Acacia nilotica表现出中等的活性，而Salvadora persica则表现出有限的活性。二次模型拟合良好，R²值在0.69 ~ 0.79之间。预测值与实验值非常吻合，误差在0.5 %以下。结论：番石榴、香薷和尼罗花在口腔护理制剂中具有很强的天然抗菌潜力。

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引用次数: 0

Tooth loss induces cognitive decline independent of low-protein diet intake in male mice 在雄性小鼠中，牙齿脱落导致认知能力下降，与低蛋白饮食摄入无关。

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2025-10-11 DOI: 10.1016/j.archoralbio.2025.106421

Rie Hatakeyama , Hiroshi Oue , Miyuki Yokoi , Eri Ishida , Takayasu Kubo , Kazuhiro Tsuga

Objectives

Previous articles have shown that tooth loss is associated with cognitive decline in animal and human studies. Additionally, poor nutritional status including low-protein diet is associated with dementia. The evidence of the association of tooth loss and nutritional status is uncertain. The purpose of this study was to investigate the relationship between low-protein diet intake and cognitive decline following tooth loss in mice.

Design

Male senescence-accelerated mouse-prone 8 mice were randomly allocated into sham-operated control and tooth loss groups with extracted maxillary molars: control with normal-protein diet; control with low-protein diet; bilateral maxillary molar extraction with normal-protein diet; and bilateral maxillary molar extraction with low-protein diet. After 6 months, a behavioral test was conducted, and mRNA expression and immunohistochemistry in the brain were analyzed.

Results

Behavioral test revealed no effect of the interaction between tooth loss and low-protein diet intake on cognitive decline; however, tooth loss had a marked effect on cognitive decline. Real-time polymerase chain reaction showed no interaction between tooth loss and low-protein intake for Bax/Bcl-2 mRNA expression; however, tooth loss had a significant effect on Bax/Bcl-2 mRNA expression. Immunohistochemical analysis showed that the effect of tooth loss in neuronal inflammation and neuronal loss were observed in CA1 and DG region, but the effect of low-protein diet was limited in CA3.

Conclusion

The present study revealed that the impact of tooth loss on cognitive decline was not dependent on the low-protein diet condition.

目的：以前的文章已经在动物和人类研究中表明，牙齿脱落与认知能力下降有关。此外，营养状况不佳，包括低蛋白饮食与痴呆症有关。牙齿脱落与营养状况之间关系的证据尚不确定。本研究的目的是探讨低蛋白饮食摄入与小鼠牙齿脱落后认知能力下降之间的关系。设计：雄性衰老加速小鼠8只，随机分为假手术对照组和拔除上颌磨牙的牙齿脱落组；对照组采用低蛋白饮食；正常蛋白饮食下双侧上颌磨牙拔牙；双侧上颌磨牙拔牙采用低蛋白饮食。6个月后进行行为学测试，分析脑内mRNA表达和免疫组化。结果：行为学测试未发现缺牙与低蛋白饮食的交互作用对认知能力下降有影响；然而，牙齿脱落对认知能力下降有显著影响。实时聚合酶链反应显示，牙齿脱落与低蛋白摄入对Bax/Bcl-2 mRNA表达无交互作用；而缺牙对Bax/Bcl-2 mRNA表达有显著影响。免疫组化分析显示，在CA1区和DG区观察到牙齿脱落对神经元炎症和神经元丢失的影响，但低蛋白饮食对CA3区的影响有限。结论：本研究揭示了牙齿脱落对认知能力下降的影响不依赖于低蛋白饮食条件。

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引用次数: 0

B. lactis HN019 modulates periapical inflammation and enhances cementum repair by increasing CEMP-1 expression in an animal model 在动物模型中，B. lactis HN019通过增加cmp -1的表达来调节根尖周围炎症并促进骨质修复。

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2025-10-10 DOI: 10.1016/j.archoralbio.2025.106420

Lisa Danielly Curcino Araujo , Ana Paula Gomes-Moura , Raquel Assed Bezerra da Silva , Michel Reis Messora , Flávia Aparecida Chaves Furlaneto , Sérgio Luis de Souza Salvador , Allan Radaic , Pachiyappan Kamarajan , Yvonne L. Kapila , Paulo Nelson-Fillho , Higinio Arzate , Samin Sirous , Geeta Bhuvanagiri , Léa Assed Bezerra da Silva

Objective

To investigate the effect of the HN019 probiotic on the modulation of inflammation and the resorption of mineralized tissues in experimentally induced periapical lesions in an animal model, evaluating its impact on the expression of cementum protein 1 (CEMP-1).

Design

Periapical lesion was induced in 45 Wistar rats. The animals were divided into groups according to the irrigating solution used. Root canals were irrigated on days 7 and 14. After 21 days, the animals were euthanized, and mandibles were processed for histological analysis. Descriptive and semi-quantitative evaluations of the inflammatory infiltrate, periodontal ligament, bone and cementum resorption, as well as counts of inflammatory cells were performed using HE staining. Also, to assess cementum formation, tissue sections were stained with an antibody for CEMP-1 and analyzed by immunohistochemistry.

Results

In the Infection + Probiotic Group, the inflammatory infiltrate was mixed and diffuse, ranging from mild to severe, with no statistically significant difference compared to the Control Group (p > 0.05). However, in the Infection + Probiotic Group, cementum resorption was significantly lower compared to Control Group (p < 0.001). Moreover, CEMP-1 expression was significantly higher in Infection + Probiotic-Group compared with all other groups (p < 0.0001).

Conclusion

The probiotic HN019 significantly increased CEMP-1 expression, indicating cementum formation in periapical lesions in vivo. These findings suggest that B. lactis HN019 may have regenerative potential in clinical scenarios. Furthermore, this strain appears to modulate the inflammatory state of periapical lesions toward a reparative phenotype, characterized by reduced bone resorption. Also, inflammatory infiltrate was like control group.

目的：通过动物模型研究HN019益生菌对实验性根尖周病变中炎症和矿化组织吸收的调节作用，评估其对骨水泥蛋白1 （cementum protein 1, cmp -1）表达的影响。设计：45只Wistar大鼠根尖周围病变。根据所使用的灌洗液进行分组。在第7天和第14天进行根管冲洗。21天后，对动物实施安乐死，并对下颌骨进行组织学分析。采用HE染色对炎症浸润、牙周韧带、骨骨质吸收、炎症细胞计数进行描述性和半定量评价。此外，为了评估骨质形成，组织切片用cmp -1抗体染色，并通过免疫组织化学分析。结果：感染+ 益生菌组炎症浸润呈混合性、弥漫性，轻重有别，与对照组比较差异无统计学意义（p > 0.05）。而感染+ 益生菌组骨质吸收明显低于对照组（p ）结论：益生菌HN019显著提高了cmp -1的表达，提示体内根尖周病变中骨质形成。这些发现提示乳杆菌HN019在临床可能具有再生潜能。此外，这种菌株似乎可以调节根尖周围病变的炎症状态，使其向修复表型发展，其特征是骨吸收减少。炎症浸润与对照组相似。

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引用次数: 0

Mirt2 alleviates LPS-induced inflammation of osteoblasts in alveolar bone destruction Mirt2可减轻lps诱导的成骨细胞在牙槽骨破坏中的炎症反应

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2025-10-09 DOI: 10.1016/j.archoralbio.2025.106410

Yajie Wu , Zhifei Su , Bowen Zhang , Lei Cheng , Ziyou Wang , He Yuan , Jiyao Li

Objectives

The long noncoding RNA (lncRNA) myocardial infarction–associated transcript 2 (Mirt2) has been confirmed to affect several inflammatory diseases. This study was conducted to explore the functional mechanism of Mirt2 in inflammatory alveolar bone loss and its possibility of being a therapeutic target.

Design

The expression level and potential role of Mirt2 in chronic inflammatory alveolar bone loss in mouse models of periodontitis and periapical periodontitis were investigated using micro-CT and qPCR. The characteristics of Mirt2 were evaluated by FISH, qPCR, ELISA, and alkaline phosphatase staining to confirm its function and mechanism of action in inflammatory response.

Results

Mirt2 expression was significantly enriched in inflammatory alveolar bone diseases. Mirt2 expression increased upon LPS stimulation in MC3T3-E1 cells (P < 0.05), located at the cell cytoplasm. Mirt2 knockdown exacerbated the LPS-stimulated inflammatory response in MC3T3-E1 cells, whereas Mirt2 overexpression attenuated this effect and rescued LPS-impaired osteogenic differentiation.

Conclusions

lncRNA Mirt2 suggests a potential role in chronic inflammation–related bone loss, providing potential therapeutic target worthy of future investigation for inflammation-related bone loss.

目的长链非编码RNA （lncRNA）心肌梗死相关转录物2 （Mirt2）已被证实影响多种炎症性疾病。本研究旨在探讨Mirt2在炎症性牙槽骨丢失中的作用机制及其作为治疗靶点的可能性。设计采用micro-CT和qPCR方法研究Mirt2在牙周炎和根尖周炎小鼠慢性炎症性牙槽骨丢失中的表达水平及其潜在作用。通过FISH、qPCR、ELISA、碱性磷酸酶染色等方法评价Mirt2的特征，确认其在炎症反应中的功能和作用机制。结果smirt2在炎症性牙槽骨疾病中表达显著富集。MC3T3-E1细胞在LPS刺激下Mirt2表达增加（P <； 0.05），位于细胞质中。Mirt2敲低加重了lps刺激的MC3T3-E1细胞的炎症反应，而Mirt2过表达减弱了这种作用，并挽救了lps受损的成骨分化。结论slncrna Mirt2可能在慢性炎症相关性骨质流失中发挥潜在作用，为炎症相关性骨质流失提供了值得未来研究的潜在治疗靶点。

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引用次数: 0

Hsa_circ_0007349 upregulates glutamate-ammonia ligase to modulate gingival fibroblast wound healing Hsa_circ_0007349上调谷氨酸氨连接酶调节牙龈成纤维细胞创面愈合

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2025-10-08 DOI: 10.1016/j.archoralbio.2025.106419

Liang Ren , Linlin He , Runyu Zhang , Xiaoyi Li , Shimeng Xiao , Shujuan Guo , Chengcheng Liu , Yi Ding

Objectives

Human gingival fibroblasts (HGFs) are important cells that maintain the structure and integrity of periodontal tissues. Circular RNAs (circRNAs) play critical roles in cellular processes of periodontitis; however, their roles in HGFs remain unclear. This study aimed to elucidate the function and mechanism of a novel circRNA, hsa_circ_0007349, in periodontitis progression by validating its existence, establishing its role in a ceRNA network (targeting miR-127–5p/GLUL), assessing its regulatory effects on HGF functions in vitro, and confirming its pathological impact in a murine periodontitis model.

Design

We performed circRNA sequencing to identify differentially expressed circRNAs between periodontitis and healthy gingival tissues. We selected hsa_circ_0007349, and confirmed its circular structure by Sanger sequencing, RNase R and actinomycin D assays. hsa_circ_0007349/miR-127–5p/glutamate-ammonia ligase (GLUL) interactions were explored using bioinformatics analysis, dual-luciferase reporter assays, RT-qPCR, and western blotting, and their effects on HGF functions were assessed via transcriptome sequencing, Cell Counting Kit-8, cell scratch, and Transwell assays. To further investigate the pathogenic mechanisms, we established a standardized murine periodontitis model using female C57BL/6 mice, followed by histopathological evaluation of periodontal tissue destruction.

Results

Hsa_circ_0007349 expression significantly increased in gingival tissues of patients with periodontitis and in HGFs treated with Porphyromonas gingivalis lipopolysaccharide. hsa_circ_0007349 regulates miR-127–5p to affect cell proliferation, migration, and wound healing, and affects extracellular matrix metabolism and periodontal tissue damage via GLUL.

Conclusion

Hsa_circ_0007349 acts as a competing endogenous RNA to promote HGF proliferation, migration, wound healing and extracellular matrix turnover by targeting miR-127–5p and GLUL.

目的：人牙龈成纤维细胞是维持牙周组织结构和完整性的重要细胞。环状rna （circRNAs）在牙周炎的细胞过程中发挥关键作用；然而，它们在hgf中的作用尚不清楚。本研究旨在通过验证一种新的circRNA hsa_circ_0007349在牙周炎进展中的功能和机制，建立其在ceRNA网络中的作用（靶向miR-127-5p /GLUL），评估其体外对HGF功能的调节作用，并确认其在小鼠牙周炎模型中的病理影响。我们进行了环状rna测序，以鉴定牙周炎和健康牙龈组织之间表达差异的环状rna。我们选择了hsa_circ_0007349，并通过Sanger测序、RNase R和放线菌素D检测证实了其环状结构。hsa_circ_0007349/ miR-127-5p /谷氨酸氨连接酶（GLUL）相互作用采用生物信息学分析、双荧光素酶报告基因检测、RT-qPCR和western blotting检测，并通过转录组测序、细胞计数试剂盒-8、细胞划痕和Transwell检测评估其对HGF功能的影响。为了进一步探讨其致病机制，我们以雌性C57BL/6小鼠为实验对象，建立了标准的小鼠牙周炎模型，并对其牙周组织破坏情况进行了组织病理学评价。结果shsa_circ_0007349在牙周炎患者的牙龈组织和牙龈卟啉单胞菌脂多糖治疗的hgf中表达显著升高。hsa_circ_0007349调节miR-127-5p影响细胞增殖、迁移和创面愈合，并通过GLUL影响细胞外基质代谢和牙周组织损伤。结论hsa_circ_0007349作为一种竞争性内源性RNA，通过靶向miR-127-5p和GLUL促进HGF增殖、迁移、伤口愈合和细胞外基质更新。

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引用次数: 0

Distinct oral microbiome profiles in substance users in the high-incidence Mizo tribal community, Northeast India 印度东北部高发病率米佐部落社区药物使用者的口腔微生物群特征

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2025-10-08 DOI: 10.1016/j.archoralbio.2025.106418

Lalfak Zuali , Lalchhanhima Ralte , John Zothanzama , Christine Vanlalbiakdiki Sailo , Nachimuthu Senthil Kumar

Objectives

To characterize the oral microbiome of substance users (primarily tobacco, alcohol, and opioids) compared to healthy controls in the high-incidence Mizo tribal community of Northeast India, and to examine their association with food and lifestyle habits.

Methods

Saliva samples from 53 substance users and 35 healthy controls were subjected to 16S rRNA (V3–V4 region) sequencing. Alpha and beta diversity analyses, differential abundance testing, and microbial functional prediction were conducted. Statistical analyses were corrected for multiple comparisons using false discovery rate (FDR) adjustment where applicable. Associations with food and lifestyle habits were also examined.

Results

Substance users exhibited significantly lower alpha diversity, and beta diversity analyses revealed distinct clustering between the two groups. Firmicutes_D, Actinobacteria, Rothia, and Streptococcus were more abundant in substance users, whereas Bacteroidota, Proteobacteria, Neisseria, and Prevotella were enriched in healthy controls. Functional predictions indicated upregulation of biofilm formation and xenobiotic degradation pathways in substance users. Exposure to Jhum cultivation, prenatal smoking, and consumption of fermented pork fat (saum) were correlated with microbial composition.

Conclusions

Substance use and associated environmental exposures were linked to oral microbiome dysbiosis. Certain bacterial taxa may serve as potential microbial biomarkers of substance use in this high-risk tribal population, warranting further investigation.

目的：在印度东北部高发病率的米佐部落社区，与健康对照者相比，描述物质使用者（主要是烟草、酒精和阿片类药物）的口腔微生物群特征，并研究它们与食物和生活习惯的关系。方法：对53名药物使用者和35名健康对照者的唾液进行16S rRNA （V3-V4区）测序。进行了α和β多样性分析、差异丰度检验和微生物功能预测。在适用的情况下，使用错误发现率（FDR）调整对多重比较进行统计分析。与食物和生活习惯的关系也被调查。结果：物质使用者表现出明显较低的α多样性，β多样性分析显示两组之间存在明显的聚类。厚壁菌属、放线菌属、罗氏菌属和链球菌在药物使用者中含量较高，而拟杆菌属、变形菌属、奈瑟菌属和普雷沃菌属在健康对照中含量较高。功能预测表明，物质使用者的生物膜形成和外源性降解途径上调。暴露于Jhum培养、产前吸烟和食用发酵猪油（saum）与微生物组成相关。结论：药物使用和相关的环境暴露与口腔微生物群失调有关。某些细菌分类群可能作为该高危部落人群物质使用的潜在微生物生物标志物，值得进一步研究。

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引用次数: 0

Enhanced regenerative potential of human dental pulp stem cells for the pulp-dentin complex through coculture with iPSC-derived endothelial cells: An in vitro study 通过与ipsc来源的内皮细胞共培养增强人牙髓干细胞对牙髓-牙本质复合体的再生潜力：一项体外研究

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2025-10-02 DOI: 10.1016/j.archoralbio.2025.106409

Hsu Myat Cho , Ukseong Kim , Sunil Kim , Stephanie Myeong Choi , Sukjoon Lee , Euiseong Kim

Objectives

Although cell-based therapies using human dental pulp stem cells (hDPSCs) with other cell lineages and growth factors show promise in regenerative endodontics, combining hDPSCs with induced pluripotent stem cell-derived endothelial cells (iPSC-ECs) is unexplored. Moreover, iPSC-ECs overcome ethical and practical challenges related to primary endothelial cells. This study explored the odontogenic and angiogenic potential of hDPSCs and iPSC-ECs in direct coculture.

Design

hDPSCs were isolated from extracted human teeth, and iPSC‑ECs were generated via episomal reprogramming of hDPSCs followed by endothelial differentiation. Four groups were established for differentiation assays: hDPSCs in basal medium, osteogenic medium, modified osteogenic medium (D‑MOD), and coculture with iPSC‑ECs (1:5) in D‑MOD. Mineralization was assessed by alkaline phosphatase and alizarin red S staining; gene expression of odontogenic (DSPP, IBSP, ALPL) and angiogenic (PECAM1, MCAM, KDR) markers was measured by RT‑qPCR; protein levels were evaluated by Western blot and nestin immunofluorescence; and angiogenic capacity in the D‑MOD and coculture groups was quantified via Matrigel tube‑formation assay.

Results

The coculture group showed enhanced mineralization and significantly increased expression of DSPP, IBSP, and PECAM1. Protein analysis confirmed elevated DSPP and nestin levels. Tube formation assays revealed significantly more junctions, segments, and meshes in the coculture group.

Conclusions

This study demonstrated in vitro that coculturing hDPSCs with iPSC-ECs enhances both odontogenic and angiogenic differentiation compared to hDPSCs cultured alone. These findings highlight the potential of iPSC technology in regenerative endodontics and indicate a promising cell-based approach for future therapeutic applications.

目的：尽管利用人牙髓干细胞（hDPSCs）与其他细胞系和生长因子的细胞为基础的治疗方法在再生牙髓学中显示出前景，但hDPSCs与诱导多能干细胞衍生内皮细胞（iPSC-ECs）的结合尚未探索。此外，iPSC-ECs克服了与原代内皮细胞相关的伦理和实践挑战。本研究探讨了hdpsc和iPSC-ECs直接共培养的成牙潜能和血管生成潜能。设计：从提取的人牙齿中分离出hdpsc，通过hdpsc的局部重编程和内皮分化生成iPSC - ECs。建立四组进行分化试验：hdpsc在基础培养基、成骨培养基、改良成骨培养基（D - MOD）中，与iPSC - ECs在D - MOD中共培养（1:5）。碱性磷酸酶和茜素红S染色评价矿化；RT - qPCR检测牙源性（DSPP、IBSP、ALPL）和血管生成性（PECAM1、MCAM、KDR）标志物的基因表达；Western blot和nestin免疫荧光法检测蛋白水平；D - MOD组和共培养组的血管生成能力通过Matrigel成管试验进行量化。结果：共培养组矿化度增强，DSPP、IBSP、PECAM1表达显著升高。蛋白分析证实DSPP和巢蛋白水平升高。试管形成试验显示，共培养组中有更多的连接、节段和网格。结论：本研究表明，与单独培养的hdpsc相比，hdpsc与iPSC-ECs共培养可增强成牙和血管分化。这些发现强调了iPSC技术在再生牙髓学中的潜力，并指出了一种有前途的基于细胞的治疗方法。

{"title":"Enhanced regenerative potential of human dental pulp stem cells for the pulp-dentin complex through coculture with iPSC-derived endothelial cells: An in vitro study","authors":"Hsu Myat Cho , Ukseong Kim , Sunil Kim , Stephanie Myeong Choi , Sukjoon Lee , Euiseong Kim","doi":"10.1016/j.archoralbio.2025.106409","DOIUrl":"10.1016/j.archoralbio.2025.106409","url":null,"abstract":"<div><h3>Objectives</h3><div>Although cell-based therapies using human dental pulp stem cells (hDPSCs) with other cell lineages and growth factors show promise in regenerative endodontics, combining hDPSCs with induced pluripotent stem cell-derived endothelial cells (iPSC-ECs) is unexplored. Moreover, iPSC-ECs overcome ethical and practical challenges related to primary endothelial cells. This study explored the odontogenic and angiogenic potential of hDPSCs and iPSC-ECs in direct coculture.</div></div><div><h3>Design</h3><div>hDPSCs were isolated from extracted human teeth, and iPSC‑ECs were generated via episomal reprogramming of hDPSCs followed by endothelial differentiation. Four groups were established for differentiation assays: hDPSCs in basal medium, osteogenic medium, modified osteogenic medium (D‑MOD), and coculture with iPSC‑ECs (1:5) in D‑MOD. Mineralization was assessed by alkaline phosphatase and alizarin red S staining; gene expression of odontogenic (<em>DSPP, IBSP, ALPL</em>) and angiogenic (<em>PECAM1, MCAM, KDR</em>) markers was measured by RT‑qPCR; protein levels were evaluated by Western blot and nestin immunofluorescence; and angiogenic capacity in the D‑MOD and coculture groups was quantified via Matrigel tube‑formation assay.</div></div><div><h3>Results</h3><div>The coculture group showed enhanced mineralization and significantly increased expression of <em>DSPP</em>, <em>IBSP</em>, and <em>PECAM1</em>. Protein analysis confirmed elevated DSPP and nestin levels. Tube formation assays revealed significantly more junctions, segments, and meshes in the coculture group.</div></div><div><h3>Conclusions</h3><div>This study demonstrated <em>in vitro</em> that coculturing hDPSCs with iPSC-ECs enhances both odontogenic and angiogenic differentiation compared to hDPSCs cultured alone. These findings highlight the potential of iPSC technology in regenerative endodontics and indicate a promising cell-based approach for future therapeutic applications.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"180 ","pages":"Article 106409"},"PeriodicalIF":2.1,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145245253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphological traits and caries susceptibility of the cusp of carabelli in permanent maxillary molars: A study in Lahore, Pakistan 巴基斯坦拉合尔地区恒上颌磨牙牙尖形态特征及龋易感性的研究

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2025-09-27 DOI: 10.1016/j.archoralbio.2025.106406

Maliha Shahbaz , Naauman Zaheer , Usman Zaheer , Madiha , Muhammad Haris Bilal , Abdullah Sajid , Junaid Ali , Hareem Aziz , Khurram Nadeem

Objective

To evaluate the prevalence, morphological traits, and caries susceptibility of the Cusp of Carabelli (CoC) in permanent maxillary molars among patients in Lahore, Pakistan.

Design

A descriptive cross-sectional study was conducted among 432 participants aged 12 years or older at Lahore Medical and Dental College. Clinical examination of maxillary first and second molars was performed using the Arizona State University Dental Anthropology System (ASUDAS) for CoC traits and the International Caries Detection and Assessment System (ICDAS) for caries assessment. Inter- and intra-examiner calibration ensured diagnostic reliability (Cohen's Kappa >0.90).

Results

CoC (ASUDAS grades 1–7) was observed in 201 individuals (46.5 %) on maxillary first molars and in 7 individuals (1.6 %) on second molars, with bilateral expression more common than unilateral. The right first molar showed a higher prevalence of CoC and caries incidence. Morphological traits ranged from subtle grooves to pronounced cusps, with small vertical grooves (ASUDAS 1) being the most frequent. Caries susceptibility correlated positively with CoC prominence (p < 0.001). Multivariate logistic regression identified CoC grade as the strongest predictor of caries, overshadowing age, side, and molar position, and substantially improving model sensitivity (0 % to 97.2 %).

Conclusion

CoC is a prevalent trait and is significantly associated with early-stage dental caries in maxillary first molars. Its presence, particularly in prominent forms, may pose an increased risk of caries. These findings underscore the need for enhanced preventive strategies and clinical attention in individuals with CoC.

目的了解巴基斯坦拉合尔地区上颌恒磨牙Carabelli尖（CoC）的患病率、形态特征及龋易感性。设计在拉合尔医学和牙科学院对432名12岁或以上的参与者进行了一项描述性横断面研究。采用亚利桑那州立大学口腔人类学系统（ASUDAS）对上颌第一、第二磨牙进行CoC特征的临床检查，采用国际龋齿检测与评估系统（ICDAS）对龋齿进行评估。审查员之间和内部的校准确保了诊断的可靠性（Cohen's Kappa >0.90）。结果上颌第一磨牙有201例（46.5 %）、第二磨牙有7例（1.6 %）出现scoc （ASUDAS分级1 ~ 7级），双侧表达较单侧多见。右第一磨牙CoC患病率和龋发病率较高。形态特征从细微的凹槽到明显的尖头，以小的垂直凹槽（ASUDAS 1）最为常见。龋易感性与CoC显著性呈正相关（p <； 0.001）。多变量logistic回归发现CoC分级是龋病、遮蔽年龄、侧边和磨牙位置的最强预测因子，并显著提高了模型敏感性（0 %至97.2% %）。结论coc是上颌第一磨牙早期龋病的普遍特征，与早期龋病有显著关系。它的存在，特别是突出的形式，可能会增加患龋的风险。这些发现强调需要加强对CoC患者的预防策略和临床关注。

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引用次数: 0