Archives of oral biology最新文献_第2页

Mimicking and in vitro validating chronic inflammation in human gingival fibroblasts 模拟和体外验证人类牙龈成纤维细胞的慢性炎症。

IF 2.2 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2024-10-19 DOI: 10.1016/j.archoralbio.2024.106113

Anne Eriksson Agger , Athina Samara , Tianxiang Geng , Ole Kristoffer Olstad , Janne Elin Reseland

Objective

The aim of this study was to identify and validate in vitro conditions that may mimic the translational, cytokine and chemokine profiles observed in human inflamed gingiva in vivo.

Design

Primary human gingiva fibroblast cells (HFIB-G) were cultured under serum starvation conditions (0 – 10 %), supplemented with increasing lipopolysaccharide (LPS) concentrations (0.1, 1, or 10 µg/ml) from two bacterial strains E. coli and P. gingivalis and 0.1, 1, or 10 ng/ml recombinant interleukin 1β (IL-1β), alone or in combinations. The levels of cytokines/chemokines were measured in the cell culture medium by Luminex, and gene expression was quantified by Affymetrix microarrays at 24, 48 and 72 h.

Results

Inflammation markers were not elevated after stimulation with P. gingivalis LPS, while E. coli LPS and IL-1β individually increased the secretion of interleukin 6 (IL-6) and monocyte chemoattractant protein-1 (MCP-1) to the cell culture medium. IL-1β administration also increased the secretion of several factors, including tumor necrosis factor (TNFα). However, the combination of 1 µg/ml E. coli LPS, 1 ng/ml IL-1β and serum starvation led to increased secretion of IL-6, TNFα, in addition to other factors found in inflamed tissue. Gene expression analyses revealed that this combination not only enhanced the expression interleukins/chemokines genes but also T helper cell signaling and matrix metalloproteinases.

Conclusion

Serum reduction in cell culture medium together with the administration of E. coli LPS and IL-1β resulted in gene expression and secreted cytokine/chemokine profiles similar to that found in vivo during chronic inflammation.

研究目的本研究旨在确定和验证体外条件，以模拟在体内发炎的人类牙龈中观察到的翻译、细胞因子和趋化因子特征：原代人牙龈成纤维细胞（HFIB-G）在血清饥饿条件（0 - 10 %）下培养，同时补充浓度不断升高的脂多糖（LPS）（0.1、1 或 10 µg/ml），脂多糖来自两种细菌菌株大肠杆菌和牙龈脓杆菌，重组白细胞介素 1β (IL-1β)浓度分别为 0.1、1 或 10 ng/ml，可单独使用或混合使用。用 Luminex 测定细胞培养液中细胞因子/凝血因子的水平，并在 24、48 和 72 小时后用 Affymetrix 芯片对基因表达进行量化：结果：用牙龈脓毒性球菌 LPS 刺激后，炎症标志物没有升高，而大肠杆菌 LPS 和 IL-1β 则分别增加了细胞培养基中白细胞介素 6（IL-6）和单核细胞趋化蛋白-1（MCP-1）的分泌。IL-1β 还能增加多种因子的分泌，包括肿瘤坏死因子（TNFα）。然而，将 1 µg/ml 大肠杆菌 LPS、1 ng/ml IL-1β 和血清饥饿结合使用会导致 IL-6、TNFα 以及炎症组织中的其他因子分泌增加。基因表达分析表明，这种组合不仅增强了白细胞介素/趋化因子基因的表达，还增强了 T 辅助细胞信号传导和基质金属蛋白酶的表达：结论：减少细胞培养基中的血清含量，同时给予大肠杆菌 LPS 和 IL-1β 会导致基因表达和分泌的细胞因子/趋化因子谱与体内慢性炎症时的情况相似。

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引用次数: 0

Impact of botulinum toxin type A on tooth movement and bone remodeling in male Wistar rats A 型肉毒毒素对雄性 Wistar 大鼠牙齿移动和骨骼重塑的影响

IF 2.2 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2024-10-18 DOI: 10.1016/j.archoralbio.2024.106105

Joana Estephany Gordillo Yépez , Renata Machado Marangon , Aline Cristina Batista Rodrigues Johann , Bruno Massa de Viveiros , Patricia Kern Di Scala Andreis , Luana Vosgerau , Sara Moreira Leal Salvação , Orlando Motohiro Tanaka , Odilon Guariza-Filho , Sergio Aparecido Ignácio , Elisa Souza Camargo

Objective

We evaluated whether the use of botulinum neurotoxin type A (BTX-A) in masticatory muscles influences tooth movement and bone remodeling.

Design

Seventy-seven male Wistar rats were allocated to the groups: S - Saline (n=20); SM - Saline with movement (n=20); BT - Botulinum toxin (n=18); BTM - Botulinum toxin with movement (n=19). On day 1, 0.02 mL of sterile 0.9 % saline was administered to groups S and SM and BTX-A (1 U in 0.02 mL of saline) to groups BT and BTM, in the masseter and temporal muscles laterally. On day 30, a nickel titanium spring was installed to move the first maxillary molar and euthanasia was performed on days 32 and 51. Tooth displacement, maxillary and mandibular bone volumes, collagen neoformation, bone and root resorptions, and masseter morphometry were assessed. Statistical analysis was conducted (p < 0.05).

Results

A higher percentage of type I collagen was observed in the BT than in the S group on day 51 and lower mass, length, and diameter of the masseter fibers in BT and BTM (p < 0.05). Tooth displacement, bone volume, bone and root resorptions, hyaline area, and masseter height showed no difference among groups with and without BTX-A, regardless of tooth movement (p > 0.05).

Conclusions

BTX-A did not interfere with tooth displacement, bone volume, and dental and periodontal tissues related to tooth movement in rats; it increased mature collagen in animals without tooth movement; and it caused a decrease in the mass, length, and diameter of the masseter fibers.

目的我们评估了在咀嚼肌中使用A型肉毒杆菌神经毒素（BTX-A）是否会影响牙齿移动和骨重塑：将 77 只雄性 Wistar 大鼠分配到不同组别：S -生理盐水组（n=20）；SM -含运动的生理盐水组（n=20）；BT -肉毒杆菌毒素组（n=18）；BTM -含运动的肉毒杆菌毒素组（n=19）。第 1 天，给 S 组和 SM 组注射 0.02 mL 0.9 % 无菌生理盐水，给 BT 组和 BTM 组注射 BTX-A（1 U 加入 0.02 mL 生理盐水中），注射部位为咀嚼肌和颞侧肌肉。第 30 天，安装镍钛弹簧以移动上颌第一磨牙，第 32 天和第 51 天实施安乐术。对牙齿移位、上颌骨和下颌骨体积、胶原新生、骨和牙根吸收以及咀嚼肌形态进行了评估。进行了统计分析（P < 0.05）：结果：第 51 天，观察到 BT 组 I 型胶原的比例高于 S 组，BT 组和 BTM 组的颌间肌纤维的质量、长度和直径均低于 S 组（P < 0.05）。牙齿移位、骨量、骨和牙根吸收、透明区和咀嚼肌高度在使用 BTX-A 和未使用 BTX-A 的组间无差异，与牙齿移动无关（P > 0.05）：结论：BTX-A 不影响大鼠牙齿移位、骨量以及与牙齿移动相关的牙齿和牙周组织；它增加了无牙齿移动动物的成熟胶原蛋白；它导致颌间肌纤维的质量、长度和直径下降。

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引用次数: 0

Evaluation of hard tissue characteristics and calcifications in pulp tissue of hypomineralized permanent molars using micro-computed tomography 使用微型计算机断层扫描评估低矿化恒磨牙牙髓组织中的硬组织特征和钙化。

IF 2.2 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2024-10-18 DOI: 10.1016/j.archoralbio.2024.106111

Didem Sakaryalı Uyar , Hazal Karslıoğlu , Mert Ocak , Hakan Hamdi Çelik

Objectives

To determine and compare pulp volume, dentin mineral density, presence of microcracks, pulp stones, and accessory canals, as well as their localizations in root regions for hypomineralized and healthy teeth.

Design

This study included 60 extracted permanent molar teeth, categorized into hypomineralized and healthy groups (n = 30 each). The hypomineralized group comprised molar teeth with limited white, yellow, or brown opacities, post-eruptive breakdown, or extensive restoration or crown damage. The healthy group included caries-free molar teeth without these characteristics. Using 3D micro-computed tomography images pulp volume, dentin mineral density, and the presence and locations of microcracks, pulp stones, and accessory canals were determined for each group. Statistical analyses were conducted using Independent T-test and Chi-square test, with significance set at p < 0.05.

Results

There was no statistically significant difference between the groups regarding pulp volume and microcracks (p ≥ 0.05). The number of accessory canals was significantly greater in the cervical (p = 0.011; p < 0.05) and middle (p = 0.010; p < 0.05) regions of the hypomineralized teeth than healthy teeth. Dentin mineral density was statistically higher in the apical, middle, and cervical root regions (p < 0.001; p < 0.05); however, the number of pulp stones was found to be greater in the cervical regions of healthy teeth compared with those with hypomineralization (p = 0.026; p < 0.05).

Conclusion

There were lower dentin mineral density measurements, a decreased number of pulp stones in the cervical region, and a greater number of accessory canals in the middle and cervical regions of hypomineralized teeth compared with healthy teeth.

目的确定并比较低矿化和健康牙齿的牙髓体积、牙本质矿物质密度、是否存在微裂缝、牙髓结石和附属管，以及它们在牙根区域的位置：本研究包括 60 颗拔出的恒磨牙，分为低矿化组和健康组（各 30 颗）。低矿化组包括有局限性白斑、黄斑或棕斑、萌出后破损或大面积修复体或牙冠损坏的磨牙。健康组包括没有这些特征的无龋磨牙。使用三维微型计算机断层扫描图像确定每组的牙髓体积、牙本质矿物质密度以及微裂缝、牙髓结石和附属管的存在和位置。统计分析采用独立 T 检验和卡方检验，显著性以 p < 0.05 为限：结果：在牙髓体积和微裂缝方面，组间差异无统计学意义（P≥0.05）。低矿化牙齿的牙颈部（p = 0.011；p < 0.05）和中部（p = 0.010；p < 0.05）的附属根管数量明显多于健康牙齿。据统计，根尖、根中部和根颈部的牙本质矿物质密度较高（p < 0.001；p < 0.05）；然而，与矿化不足的牙齿相比，健康牙齿根颈部的牙髓结石数量较多（p = 0.026；p < 0.05）：结论：与健康牙齿相比，低矿化牙齿的牙本质矿物质密度测量值较低，牙颈部的牙髓结石数量较少，中间和牙颈部的附属管数量较多。

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引用次数: 0

Analysis of dental chipping for identifying and diagnosing tooth fracture patterns in osteological series 在骨学系列中分析牙齿缺损以识别和诊断牙齿断裂模式。

IF 2.2 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2024-10-18 DOI: 10.1016/j.archoralbio.2024.106114

Á. Rubio Salvador , S.A. Jiménez-Brobeil , M. Lozano

Objective

To develop a specific methodology for identifying dental chipping and determining its temporal occurrence in past populations.

Design

The analysed sample comprised of 2191 human teeth from various Bronze Age on the Iberian Peninsula (Argar culture, 1900–1450 cal BC). Among these, 471 chipped teeth were identified. Chipping was examined using various microscopic techniques (digital three-dimensional, optical, and confocal), focusing on distribution, morphology, position in the tooth, extent of damage, and post-chipping antemortem modifications (PCAM).

Results

The distribution and morphology of the chips enabled the identification chipping mechanism of the chipping, providing valid criteria to distinguish between antemortem and postmortem chipping. Microscopic analyses of the chipping segments—edges, sidewalls, surface, and surrounding area—facilitated determination of the time the chip ocurred (antemortem: recent, less recent, or not recent).

Conclusions

While experimental studies provide valuable insights into chipping mechanisms, many criteria may not be applicable to past populations because of the presence of PCAM. The lack of PCAM in some Argaric teeth suggests that previous studies may have underestimated the prevalence of chipping in past populations.

目的：制定识别牙齿缺损的具体方法，并确定其在过去人群中出现的时间：设计：分析的样本包括来自伊比利亚半岛不同青铜时代（阿尔加文化，公元前 1900-1450 年）的 2191 颗人类牙齿。在这些牙齿中，有 471 颗牙齿有缺口。我们使用各种显微技术（数字三维、光学和共聚焦）对崩裂进行了检查，重点是崩裂的分布、形态、在牙齿中的位置、损坏程度以及崩裂后的死前改造（PCAM）：碎屑的分布和形态有助于确定碎屑的碎裂机制，为区分死前碎屑和死后碎屑提供了有效的标准。对崩裂片段--边缘、侧壁、表面和周围区域--的显微分析有助于确定崩裂发生的时间（死前：近期、较近期或非近期）：尽管实验研究为了解崩解机制提供了宝贵的资料，但由于 PCAM 的存在，许多标准可能并不适用于过去的人群。一些阿尔加里克人的牙齿中缺乏 PCAM，这表明以前的研究可能低估了崩蚀在过去人群中的发生率。

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引用次数: 0

Effects of icariin on dental pulp stem cells and its potential applications in dentin repair 冰片素对牙髓干细胞的影响及其在牙本质修复中的潜在应用。

IF 2.2 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2024-10-17 DOI: 10.1016/j.archoralbio.2024.106112

Ahmed Elhakim , Ukseong Kim , Euiseong Kim , Sukjoon Lee , Jong-Min Lee , Han-Sung Jung , Sunil Kim

Objectives

As dental pulp therapy evolves towards regenerative approaches, biomolecules such as icariin, derived from Epimedium flowers, are being evaluated for their therapeutic potential. This study investigates icariin's effectiveness in promoting odontogenic differentiation in human dental pulp stem cells (hDPSCs) in vitro and as a pulp-capping agent in vivo.

Design

The study explored the effects of icariin on hDPSCs at concentrations of 10, 20, and 40 µM. Cell viability and migration assays were conducted to evaluate cytotoxicity and chemotaxis. Odontogenic differentiation was assessed using alkaline phosphatase staining and alizarin red S (ARS) staining, complemented by real-time PCR and Western blot analyses of key markers such as RUNX family transcription factor 2 (RUNX2), collagen type I alpha 1 chain (COL1A1), alkaline phosphatase (ALPL), and dentin sialophosphoprotein (DSPP). Additionally, the in vivo effects of icariin were tested in a rat maxillary molar model, where icariin-treated collagen sponges were used for direct pulp capping to evaluate its potential to induce reparative dentin formation.

Results

Icariin showed no cytotoxic effects on hDPSCs at any tested concentration, enhanced migratory activity in a dose-dependent manner, and significantly increased alkaline phosphatase activity and calcium deposition. Gene and protein expression analyses revealed a dose-dependent increase in odontogenic differentiation markers in icariin-treated hDPSCs. In vivo, icariin effectively promoted reparative dentin formation in exposed rat pulp.

Conclusions

Icariin enhances odontogenic differentiation of hDPSCs and has promising potential as a pulp-capping agent for vital pulp therapy.

目的：随着牙髓治疗向再生方法发展，从淫羊藿花卉中提取的生物大分子（如冰片苷）的治疗潜力正在接受评估。本研究调查了冰片在体外促进人牙髓干细胞（hDPSCs）牙源分化以及在体内作为牙髓覆盖剂的有效性：本研究探讨了浓度为 10、20 和 40 µM 的冰片花素对 hDPSCs 的影响。通过细胞活力和迁移试验来评估细胞毒性和趋化性。使用碱性磷酸酶染色和茜素红 S（ARS）染色评估牙源性分化，并对 RUNX 家族转录因子 2（RUNX2）、胶原 I 型α1 链（COL1A1）、碱性磷酸酶（APL）和牙本质纤溶磷蛋白（DSPP）等关键标记物进行实时 PCR 和 Western 印迹分析。此外，还在大鼠上颌磨牙模型中测试了伊卡丽素的体内效应，使用伊卡丽素处理过的胶原海绵直接覆盖牙髓，以评估其诱导修复性牙本质形成的潜力：结果：在任何测试浓度下，淫羊藿苷都不会对 hDPSCs 产生细胞毒性作用，它能以剂量依赖性方式增强迁移活性，并显著提高碱性磷酸酶活性和钙沉积。基因和蛋白质表达分析表明，经冰醋酸处理的 hDPSCs 中牙本质分化标记物的增加呈剂量依赖性。在体内，冰片素能有效促进暴露的大鼠牙髓中修复性牙本质的形成：淫羊藿苷增强了 hDPSCs 的牙本质分化，有望作为一种牙髓覆盖剂用于牙髓治疗。

{"title":"Effects of icariin on dental pulp stem cells and its potential applications in dentin repair","authors":"Ahmed Elhakim , Ukseong Kim , Euiseong Kim , Sukjoon Lee , Jong-Min Lee , Han-Sung Jung , Sunil Kim","doi":"10.1016/j.archoralbio.2024.106112","DOIUrl":"10.1016/j.archoralbio.2024.106112","url":null,"abstract":"<div><h3>Objectives</h3><div>As dental pulp therapy evolves towards regenerative approaches, biomolecules such as icariin, derived from <em>Epimedium</em> flowers, are being evaluated for their therapeutic potential. This study investigates icariin's effectiveness in promoting odontogenic differentiation in human dental pulp stem cells (hDPSCs) <em>in vitro</em> and as a pulp-capping agent <em>in vivo</em>.</div></div><div><h3>Design</h3><div>The study explored the effects of icariin on hDPSCs at concentrations of 10, 20, and 40 µM. Cell viability and migration assays were conducted to evaluate cytotoxicity and chemotaxis. Odontogenic differentiation was assessed using alkaline phosphatase staining and alizarin red S (ARS) staining, complemented by real-time PCR and Western blot analyses of key markers such as RUNX family transcription factor 2 (<em>RUNX2</em>), collagen type I alpha 1 chain (<em>COL1A1</em>), alkaline phosphatase (<em>ALPL</em>), and dentin sialophosphoprotein (<em>DSPP</em>). Additionally, the <em>in vivo</em> effects of icariin were tested in a rat maxillary molar model, where icariin-treated collagen sponges were used for direct pulp capping to evaluate its potential to induce reparative dentin formation.</div></div><div><h3>Results</h3><div>Icariin showed no cytotoxic effects on hDPSCs at any tested concentration, enhanced migratory activity in a dose-dependent manner, and significantly increased alkaline phosphatase activity and calcium deposition. Gene and protein expression analyses revealed a dose-dependent increase in odontogenic differentiation markers in icariin-treated hDPSCs. <em>In vivo</em>, icariin effectively promoted reparative dentin formation in exposed rat pulp.</div></div><div><h3>Conclusions</h3><div>Icariin enhances odontogenic differentiation of hDPSCs and has promising potential as a pulp-capping agent for vital pulp therapy.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"169 ","pages":"Article 106112"},"PeriodicalIF":2.2,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142514511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DNA damage and cell death in human oral squamous cell carcinoma cells: The potential biological effects of cannabidiol 人类口腔鳞状细胞癌细胞的 DNA 损伤和细胞死亡：大麻二酚的潜在生物效应。

IF 2.2 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2024-10-16 DOI: 10.1016/j.archoralbio.2024.106110

Monia Billi , Stefano Pagano , Gian Luca Pancrazi , Chiara Valenti , Stefano Bruscoli , Alessandro Di Michele , Marta Febo , Francesco Grignani , Lorella Marinucci

Objective

The present study examined the in vitro effects on oral squamous cell carcinoma cells (HSC-3) of cannabidiol (CBD), the main chemical component of Cannabis, proposed as a novel adjuvant therapy in the treatment of cancers.

Design

Cell viability (MTT assay), morphology (SEM), apoptosis and cell cycle (flow cytometry), and DNA damage (phospho-γ-H2AX immunofluorescence) were evaluated. Cytotoxicity was evaluated with concentrations between 100 µM and 1 µM, and two concentrations were selected for subsequent analysis: 25 µM, as toxic dose, and 6.25 µM, as non-toxic.

Results

CBD caused a dose- and time-dependent reduction in viability of 64 %, 96 %, and 99 % with 25 µM, 50 µM and 100 µM, respectively, after 72 h (p < 0.001), cell cycle arrest in G0-G1 phase with increased apoptosis in particular at 72 h for 25 µM (p < 0.001), significant morphological alterations with 25 µM, still present even at 6.25 µM, and significantly increased cell damage considering a significant increase in the percentage of highly positive cells (5 phosphorylated γH2AX foci), which is around 29 % for 25 µM and 19 % for 6.25 µM after 24 h.

Conclusions

CBD inhibits oral cancer growth causing DNA damage. In general, induced cell cytotoxicity appears to be dose- and time-related. Doses of CBD ≥25 μM showed a high reduction in viability. CBD could possibly represent a new therapeutic molecule for its cytotoxic effects against oral squamous cell carcinoma. The mechanism involved in the suppressive effect caused by CBD needs further investigation.

研究目的本研究探讨了大麻二酚（CBD）对口腔鳞状细胞癌细胞（HSC-3）的体外影响，大麻二酚是大麻的主要化学成分，被认为是治疗癌症的一种新型辅助疗法：设计：评估细胞活力（MTT 试验）、形态（SEM）、凋亡和细胞周期（流式细胞术）以及 DNA 损伤（phospho-γ-H2AX 免疫荧光）。细胞毒性的评估浓度为 100 µM 至 1 µM，并选择了两个浓度进行后续分析：25 µM（有毒剂量）和 6.25 µM（无毒剂量）：72小时后，25 µM、50 µM和100 µM的CBD分别导致64%、96%和99%的存活率下降（p < 0.001），细胞周期停滞在G0-G1期，细胞凋亡增加，特别是在72小时后，25 µM的细胞凋亡增加（p < 0.001），25 µM时细胞形态发生明显改变，即使在6.25 µM时也是如此，细胞损伤显著增加，因为高阳性细胞（5个磷酸化γH2AX病灶）的百分比显著增加，24小时后，25 µM时约为29%，6.25 µM时约为19%：结论：CBD 可抑制口腔癌的生长，造成 DNA 损伤。一般来说，诱导的细胞毒性似乎与剂量和时间有关。CBD ≥25 μM 的剂量可显著降低细胞活力。CBD可能是一种新的治疗分子，它对口腔鳞状细胞癌具有细胞毒性作用。CBD产生抑制作用的机制有待进一步研究。

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引用次数: 0

Melanoma-inhibiting activity promotes the migration and odontoblastic differentiation of stem cells of apical papilla 黑色素瘤抑制活性可促进根尖乳头干细胞的迁移和牙骨质分化。

IF 2.2 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2024-10-16 DOI: 10.1016/j.archoralbio.2024.106109

Huihui Ren , Qingxuan Zhao , Nan Wang , Xiaojing Yuan , Rui Song , Quan Wen , Yuming Zhao

Objective

Melanoma Inhibitory Activity (MIA) has been predominantly studied in the context of melanoma and cartilage development. However, its role in dental pulp development and stem cell behavior remains largely unexplored. This study investigates the expression pattern of MIA in dental pulp tissues and its potential role in the proliferation, migration, and odontoblastic differentiation of stem cells from the apical papilla (SCAPs).

Design

MIA expression in human pulp tissue was demonstrated by immunohistochemistry. SCAPs were cultured in normal and mineralization induction media, with MIA levels monitored via RT-qPCR and Western blot. Cell proliferation was evaluated using the CCK8 assay, while transwell and cell scratch assays were conducted to examine cell migration. The effect of MIA on odontoblastic differentiation was examined by qRT-PCR, Alkaline phosphatase activity assay, and Western blot. siRNA was used to knock down MIA to investigate its effect. A mouse subcutaneous implantation model was used to assess whether MIA promotes odontoblastic differentiation in vivo.

Results

MIA expression was observed in the papilla and odontoblasts layer of the developing pulp. In vitro, MIA expression increased during SCAPs differentiation and was found to significantly enhance migration, and odontoblastic differentiation but not proliferation. Gene knockdown experiments confirmed MIA’s pivotal role in promoting SCAPs migration and differentiation. In vivo, MIA facilitated the formation of dentin-like structures and enhanced pulp-dentin complex regeneration.

Conclusion

MIA plays a crucial role in SCAPs’ migration and differentiation, suggesting its potential application in pulp-dentin regeneration therapies. Further studies are required to fully elucidate the underlying mechanisms.

目的：黑色素瘤抑制活性（MIA）主要是在黑色素瘤和软骨发育的背景下进行研究的。然而，它在牙髓发育和干细胞行为中的作用在很大程度上仍未得到探索。本研究调查了MIA在牙髓组织中的表达模式及其在牙髓根尖乳头干细胞（SCAPs）的增殖、迁移和牙髓分化中的潜在作用：设计：通过免疫组化方法证实 MIA 在人类牙髓组织中的表达。在正常培养基和矿化诱导培养基中培养SCAPs，通过RT-qPCR和Western印迹监测MIA水平。细胞增殖采用 CCK8 试验进行评估，细胞迁移则采用跨孔试验和细胞划痕试验进行检测。通过 qRT-PCR、碱性磷酸酶活性检测和 Western 印迹检测了 MIA 对骨骺分化的影响。用小鼠皮下植入模型评估MIA是否促进了体内颌骨的分化：结果：在发育中的牙髓乳头和牙本质层观察到了MIA的表达。在体外，MIA的表达在SCAPs分化过程中增加，并被发现能显著促进迁移和牙本质分化，但不能促进增殖。基因敲除实验证实了 MIA 在促进 SCAPs 迁移和分化中的关键作用。在体内，MIA 促进了牙本质样结构的形成，并增强了牙髓-牙本质复合体的再生能力：结论：MIA 在 SCAPs 的迁移和分化中起着关键作用，这表明它有可能应用于牙髓-牙本质再生疗法。结论：MIA 对 SCAPs 的迁移和分化起着关键作用，这表明它有可能应用于牙髓-牙本质再生疗法。

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引用次数: 0

Vitamin D serum levels and temporomandibular disorders: A systematic review and meta-analysis 维生素 D 血清水平与颞下颌关节紊乱：系统回顾与荟萃分析。

IF 2.2 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2024-10-15 DOI: 10.1016/j.archoralbio.2024.106108

Reza Tabrizi , Hooman Khanzadeh , Seyed Sepehr Mirebeigi Jamasbi , Fatemeh Rezaei , Ali Azadi

Objective

This systematic review evaluates the connection between vitamin D serum levels, deficiency, and temporomandibular disorders (TMD), offering a meta-analysis of the existing evidence in this domain.

Design

The Scopus, ISI Web of Science, and Pubmed databases were searched for human studies concerning the connection between vitamin D and TMD comprising a control group. A random-effect model with forest plots was used for vitamin D serum levels mean difference (MD), vitamin D deficiency odds ratio (OR), and risk difference (RD) between subjects with and without TMD. Subgroup analysis was conducted based on ethnicity, overall risk of bias, TMD diagnosis method, and study designs. A p-value lower than 0.05 was considered significant. The certainty of the meta-evidence was evaluated according to the GRADE approach.

Results

Of the 2621 identified unique records, 15 studies were included in the study, eight of which were considered for the meta-analysis. The meta-analysis revealed a significant vitamin D deficiency OR (3.85; 95 % CI: 2.35 – 5.43; Certainty: Low) and RD (22 %; 95 % CI: 11 % - 32 %; Certainty: Very low), and vitamin D serum levels MD (-5.03 ng/mL; 95 % CI: −9.92 – −0.13; Certainty: Very low) between subjects with and without TMD. Among subgroup analyses, only the difference in vitamin D MD between Middle Eastern and European patients was significant (P < 0.01).

Conclusion

Considering the low to very low certainty of the evidence, vitamin D serum levels are significantly lower, and vitamin D deficiency is significantly more prevalent in TMD patients.

目的：本系统综述评估了维生素 D 血清水平、缺乏症和颞下颌关节紊乱症（TMD）之间的关系，并提供了该领域现有证据的荟萃分析：本系统综述评估了维生素 D 血清水平、缺乏症和颞下颌关节紊乱症（TMD）之间的联系，并对该领域的现有证据进行了荟萃分析：设计：在 Scopus、ISI Web of Science 和 Pubmed 数据库中搜索了有关维生素 D 与 TMD 之间关系的人类研究，其中包括一个对照组。对患有和未患有 TMD 的受试者之间的维生素 D 血清水平平均差 (MD)、维生素 D 缺乏症几率比 (OR) 和风险差 (RD) 采用了带森林图的随机效应模型。根据种族、总体偏倚风险、TMD 诊断方法和研究设计进行了分组分析。P 值低于 0.05 即为显著。根据 GRADE 方法对元证据的确定性进行了评估：在已识别的 2621 条唯一记录中，有 15 项研究被纳入研究，其中 8 项被考虑进行荟萃分析。荟萃分析显示，有 TMD 和没有 TMD 的受试者之间存在显著的维生素 D 缺乏 OR（3.85；95 % CI：2.35 - 5.43；确定性：低）和 RD（22 %；95 % CI：11 % - 32 %；确定性：极低），以及维生素 D 血清水平 MD（-5.03 ng/mL；95 % CI：-9.92 - 0.13；确定性：极低）。在亚组分析中，只有中东和欧洲患者的维生素 D MD 差异显著（P < 0.01）：考虑到证据的确定性较低至很低，TMD 患者的维生素 D 血清水平明显较低，维生素 D 缺乏症的发病率明显较高。

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引用次数: 0

Interaction between polymorphisms in TNF-⍺ and RANKL genes is associated with the development of persistent apical periodontitis, in Brazilian subjects 在巴西受试者中，TNF-⍺和 RANKL 基因多态性之间的相互作用与持续性根尖牙周炎的发展有关。

IF 2.2 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2024-10-10 DOI: 10.1016/j.archoralbio.2024.106106

Igor Bassi Ferreira Petean , Alice Corrêa Silva-Sousa , Guido Artemio Marañón-Vásquez , Francisco Wanderley Garcia de Paula-Silva , Erika Calvano Küchler , Leonardo Santos Antunes , Raquel Assed Bezerra Segato , Lea Assed Bezerra da Silva , Jardel Francisco Mazzi-Chaves , Fabiane Carneiro Lopes-Olhê , Manoel Damião Sousa-Neto

Objectives

To investigate the association between genetic polymorphisms in suppressor of cytokine signaling-1 (SOCS-1), tumor necrosis factor-⍺ (TNF-α) and its receptors 1 and 2 (TNFRSF1A and TNFRSF1B), receptor activator of nuclear factor kappa-b (RANK), receptor activator of nuclear factor-kappa B ligand (RANKL) and osteoprotegerin (OPG), and persistent apical periodontitis (PAP).

Methods

Patients with pulp necrosis and apical periodontitis at the time of non-surgical root canal treatment were followed up for at least one year. A total of 423 subjects were included, 172 with signs/symptoms of PAP and 251 with apical periodontitis healed. DNA was extracted from saliva and used for genotyping polymorphisms in SOCS1 (rs243327 and rs33977706), TNF-α (rs1800629), TNFRSF1A (rs1800693), TNFRSF1B (rs1061622), RANK (rs1805034), RANKL (rs1054016) and OPG (rs1032128) by real-time PCR. The frequency of genotypes and alleles was assessed using chi-squared test or Fisher's exact test and odds ratio. Interactions were also tested using multifactor dimensionality reduction (α = 5 %).

Results

In the polymorphism rs1800629 in TNF-α, carrying at least one A risk allele significantly decreased the risk to develop PAP (OR=0.47; 95 %CI: 0.28–0.79; p=0.004). For the polymorphism rs1054016 in RANKL carrying both T risk alleles significantly decreased the risk to develop PAP (OR=0.47; 95 %CI: 0.24–0.92; p=0.027). None of the other polymorphisms evaluated were associated with PAP (p>0.05). A strong interaction was observed among rs1800629, rs1061622 and rs1054016.

Conclusions

Genetic polymorphisms rs1800629 (TNF-α) and rs1054016 (RANKL) had a strong interaction and were associated with a lower risk to develop PAP.

目的研究细胞因子信号转导抑制因子-1（SOCS-1）、肿瘤坏死因子-237A(TNF-α)及其受体1和2（TNFRSF1A和TNFRSF1B）的遗传多态性之间的关联、核因子卡巴-b 受体激活剂（RANK）、核因子卡巴-B 受体激活剂配体（RANKL）和骨保护素（OPG）以及持续性根尖牙周炎（PAP）。研究方法对接受非手术根管治疗时出现牙髓坏死和根尖牙周炎的患者进行至少一年的随访。共纳入 423 名受试者，其中 172 人有牙髓坏死的迹象/症状，251 人的根尖牙周炎已痊愈。研究人员从唾液中提取 DNA，并通过实时 PCR 对 SOCS1（rs243327 和 rs33977706）、TNF-α（rs1800629）、TNFRSF1A（rs1800693）、TNFRSF1B（rs1061622）、RANK（rs1805034）、RANKL（rs1054016）和 OPG（rs1032128）的多态性进行基因分型。使用卡方检验或费雪精确检验和几率比验评估基因型和等位基因的频率。还使用多因素降维法（α = 5 %）对相互作用进行了测试：结果：在 TNF-α 的多态性 rs1800629 中，携带至少一个 A 风险等位基因可显著降低罹患 PAP 的风险（OR=0.47；95 %CI：0.28-0.79；p=0.004）。对于 RANKL 的多态性 rs1054016，携带两个 T 风险等位基因可明显降低罹患 PAP 的风险（OR=0.47；95 %CI：0.24-0.92；p=0.027）。所评估的其他多态性均与 PAP 无关（P>0.05）。在 rs1800629、rs1061622 和 rs1054016 之间观察到强烈的交互作用：结论：rs1800629（TNF-α）和rs1054016（RANKL）的基因多态性具有很强的交互作用，与较低的PAP发病风险相关。

{"title":"Interaction between polymorphisms in TNF-⍺ and RANKL genes is associated with the development of persistent apical periodontitis, in Brazilian subjects","authors":"Igor Bassi Ferreira Petean , Alice Corrêa Silva-Sousa , Guido Artemio Marañón-Vásquez , Francisco Wanderley Garcia de Paula-Silva , Erika Calvano Küchler , Leonardo Santos Antunes , Raquel Assed Bezerra Segato , Lea Assed Bezerra da Silva , Jardel Francisco Mazzi-Chaves , Fabiane Carneiro Lopes-Olhê , Manoel Damião Sousa-Neto","doi":"10.1016/j.archoralbio.2024.106106","DOIUrl":"10.1016/j.archoralbio.2024.106106","url":null,"abstract":"<div><h3>Objectives</h3><div>To investigate the association between genetic polymorphisms in suppressor of cytokine signaling-1 (<em>SOCS-1</em>)<em>,</em> tumor necrosis factor-⍺ (<em>TNF-α</em>) and its receptors 1 and 2 (<em>TNFRSF1A</em> and <em>TNFRSF1B</em>), receptor activator of nuclear factor kappa-b (<em>RANK</em>), receptor activator of nuclear factor-kappa B ligand (<em>RANKL</em>) and osteoprotegerin (<em>OPG</em>), and persistent apical periodontitis (PAP).</div></div><div><h3>Methods</h3><div>Patients with pulp necrosis and apical periodontitis at the time of non-surgical root canal treatment were followed up for at least one year. A total of 423 subjects were included, 172 with signs/symptoms of PAP and 251 with apical periodontitis healed. DNA was extracted from saliva and used for genotyping polymorphisms in <em>SOCS1</em> (rs243327 and rs33977706), <em>TNF-α</em> (rs1800629), <em>TNFRSF1A</em> (rs1800693), <em>TNFRSF1B</em> (rs1061622), <em>RANK</em> (rs1805034), <em>RANKL</em> (rs1054016) and <em>OPG</em> (rs1032128) by real-time PCR. The frequency of genotypes and alleles was assessed using chi-squared test or Fisher's exact test and odds ratio. Interactions were also tested using multifactor dimensionality reduction (<em>α</em> = 5 %).</div></div><div><h3>Results</h3><div>In the polymorphism rs1800629 in <em>TNF-</em>α, carrying at least one A risk allele significantly decreased the risk to develop PAP (OR=0.47; 95 %CI: 0.28–0.79; <em>p</em>=0.004). For the polymorphism rs1054016 in <em>RANKL</em> carrying both T risk alleles significantly decreased the risk to develop PAP (OR=0.47; 95 %CI: 0.24–0.92; <em>p</em>=0.027). None of the other polymorphisms evaluated were associated with PAP (<em>p</em>>0.05). A strong interaction was observed among rs1800629, rs1061622 and rs1054016.</div></div><div><h3>Conclusions</h3><div>Genetic polymorphisms rs1800629 (<em>TNF-α)</em> and rs1054016 (<em>RANKL)</em> had a strong interaction and were associated with a lower risk to develop PAP.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"169 ","pages":"Article 106106"},"PeriodicalIF":2.2,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142482847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Masticatory function and three-dimensional facial morphology of soft tissues: One year after orthognathic surgery 咀嚼功能和面部软组织的三维形态：正颌手术一年后

IF 2.2 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2024-10-09 DOI: 10.1016/j.archoralbio.2024.106103

Joana Carolina Martins Simões , Denny Marcos Garcia , Francisco Veríssimo De Mello-Filho , Claudia Maria De Felício , Luciana Vitaliano Voi Trawitzki

Objective

Dentofacial deformities (DFD) require orthodontic treatment, orthognathic surgery, and speech therapy for aesthetic and functional problems. This longitudinal study analyzed changes in masticatory function and three-dimensional (3D) facial soft tissue in patients with Class II and Class III DFD after orthognathic surgery. In addition, the study investigated the relationship between facial measurements, maximum bite force (MBF), and orofacial myofunctional status (OMS).

Design

The sample consisted of 46 participants, including 10 patients with Class II DFD, and 11 patients with Class III DFD. These groups were assessed before (T0) and 6 months (T1) after surgery. Twelve patients who completed the treatment protocol were evaluated 12 months post-surgery (T2). The patient groups were compared with each other and with a control group (CG) of 25 healthy subjects. The participants underwent MBF and clinical evaluation of OMS, including masticatory behavior, using a validated protocol. The 3D facial soft tissue was assessed using laser scanning.

Results

Compared to the CG, DFD patients showed reduced MBF, masticatory behavior, and OMS scores at T0. At T2, there was an improvement in MBF, masticatory, and OMS scores, but differences were observed compared to the CG. Changes in facial soft tissue followed the underlying hard tissue movement resulting from surgery, and reduction of vertical measurements contributed to improvements in masticatory function.

Conclusion

These findings indicated that combined surgery, orthodontic treatment, and speech therapy can lead to a significant improvement in masticatory function and facial soft tissue in DFD patients, although not completely 12 months post-surgery.

目的：牙面畸形（DFD）需要通过正畸治疗、正颌手术和言语治疗来解决美观和功能问题。这项纵向研究分析了 II 级和 III 级 DFD 患者在正颌手术后咀嚼功能和三维（3D）面部软组织的变化。此外，该研究还调查了面部测量值、最大咬合力（MBF）和口面部肌功能状态（OMS）之间的关系：样本由 46 名参与者组成，包括 10 名 II 级 DFD 患者和 11 名 III 级 DFD 患者。这两组患者分别在手术前（T0）和手术后 6 个月（T1）接受评估。对完成治疗方案的 12 名患者进行了术后 12 个月（T2）的评估。患者组之间以及患者组与由 25 名健康人组成的对照组（CG）进行了比较。参试者接受了 MBF 和 OMS 临床评估，包括咀嚼行为，采用的是经过验证的方案。使用激光扫描技术对三维面部软组织进行了评估：结果：与CG相比，DFD患者在T0时的MBF、咀嚼行为和OMS评分均有所下降。T2时，MBF、咀嚼行为和OMS评分有所改善，但与CG相比仍有差异。面部软组织的变化与手术导致的潜在硬组织运动一致，垂直测量的减少有助于咀嚼功能的改善：这些研究结果表明，联合手术、正畸治疗和言语治疗可显著改善 DFD 患者的咀嚼功能和面部软组织，尽管在术后 12 个月仍未完全改善。

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引用次数: 0