Archives of oral biology最新文献_第2页

Evaluating the effects of L-carnitine on albino rat's gingiva-derived stem cells (In-Vitro Study)

IF 2.2 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2025-02-10 DOI: 10.1016/j.archoralbio.2025.106192

Elham H. Ahmed , Sara Mohamed Farrag , Noura Abd El-Latif

Objective

Stem cells as therapy is currently a well-established scientific research topic. Poor maintenance and survival of cells supplied to the damaged tissue are barriers to improving the efficacy of regenerative medicine. Antioxidants such as L-carnitine are used to promote cell survival and maintenance properties. This study aims to assess the effects of L-carnitine on albino rat gingiva-derived mesenchymal stem cells proliferation.

Design

Rat gingiva-derived mesenchymal stem cells were isolated and exposed to 0, 1, 3, and 10 Mm of L-carnitine. Flow cytometry was then utilized to measure gene and protein expression levels for CD90, CD105, CD45, and CD19. The MTT test was used to examine the proliferation of cells. The proportion of apoptosis was determined using the Annexin V/PI technique. Cell cycle investigations to assess cells and identify the percentages of cells in the G0/G1, S, and G2/M phases. Expression of TGF-β gene has been evaluated using Real time‑PCR analysis.

Results

The results showed that gingiva-derived mesenchymal stem cells, including CD90 and CD105, consistently showed positive immunostaining, whereas CD45 and CD19 were weakly positive or negative. Concentration-dependent increase of growth proliferation, more rapid proliferation of the cells treated with the highest L-carnitine concentration (10 mM) after 72 h (0.934 ± 0.063). Cells treated with 10 mM L-carnitine showed considerably decreased percentages of necrotic (2.38 ± 0.55), late (1.23 ± 0.90), early apoptotic cells (1.18 ± 0.13), and increased the percentage of viable cells (95.13 ± 1.61).

Conclusion

Our findings suggest that adding L-carnitine to gingiva-derived mesenchymal stem cells during expansion enables efficient and viable cell production.

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引用次数: 0

Raman confocal microscopy atlas of human tooth

IF 2.2 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2025-02-08 DOI: 10.1016/j.archoralbio.2025.106189

P.-Y. Collart-Dutilleul , S. Piglionico , R. Younes , Y. Messat , S. Garrabé , H. Salehi , J.-C. Durand , F. Cuisinier , A. Desoutter

Objective

Dental histology is a field that has been studied since the early 19th century. Most of the techniques used have been based on white-light microscopy or histological staining, or more recently immunohistochemical staining. With the advent of lasers coupled to confocal microscopy, Raman spectra can be measured in large numbers to create a detailed chemical atlas of the human tooth, offering new insights into its composition and structure.

Design

A total of twenty teeth, with 5 teeth from each type premolar, molar, incisor and canine were selected. Five teeth of different types pre-molar and molar incisor canine (total of 20 teeth) These teeth were sectioned and polished, and pulps extracted and cut into thin layers, to perform chemical mapping of all these tissues and components, including secondary structures, based on Raman scattering.

Results

We obtained images reconstructed from the intensities of the various characteristic peaks, enabling us to create an atlas of the tooth. A part of the result confirm previous study, but some structures have been revealed for the first time by chemical cartography: cementum dentin junction, dental pulp, Retzius striae, scallop pattern, Hunter-Schreger bands, sheat enamel prism content and dentin branches.

Conclusion

The present study thus provides the dental research and practice community with a complete chemical mapping of the fundamental and secondary constituents of the dental organ, with optical resolution.

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引用次数: 0

The biocompatibility, penetrability, sealing ability, and antibacterial properties of iRoot SP compared to AH plus: An In Vitro evaluation

IF 2.2 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2025-02-07 DOI: 10.1016/j.archoralbio.2025.106188

Yiling Li , Bolei Li , Siyue Lai , Xiao Guo , Yu Fan , Haohao Wang , Lei Cheng

Objectives

This study aimed to evaluate the biocompatibility, penetrability, sealing ability, and antibacterial properties of iRoot SP compared to AH Plus in root canal filling.

Design

Two types of iRoot SP and AH Plus were used for in vivo evaluation. The solubility of sealers was tested according to the International Organization for Standardization (ISO). Cell Counting kit-8 was performed for biocompatibility analysis. To evaluate the sealing efficacy, 27 extracted human single-rooted teeth were treated with the single-cone obturation technique. The penetrability was analyzed using confocal laser scanning microscope and the sealing ability was assessed through micro-computed tomography. The biofilm of Enterococcus faecalis was formed on the coagulated root canal sealers to determine the antibacterial properties by colony-forming units and scanning electronic microscopy.

Results

All sealers exhibited standard solubility (<3 %). The biocompatibility of the three root canal sealers was excellent with no statistically significant differences compared to the control group (P > 0.05). The penetrability of both iRoot SP variants was greater than that of AH Plus, especially in the apical third of teeth (P < 0.001). No significant differences in sealing efficacy among the sealers were observed (P > 0.05). IRoot SP also reduced Enterococcus faecalis counts and inhibited the biofilm formation compared to AH Plus (P < 0.01).

Conclusions

IRoot SP exhibited good biocompatibility. It has superior penetrability and enhanced antibacterial properties compared to AH Plus, but shown no difference in sealing ability, warranting further in vivo studies and long-term assessments. The research underscores the strong potential of bioceramic sealers for clinical applications.

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引用次数: 0

Extracellular polysaccharide-rich biofilm reduces chlorhexidine effect on enamel demineralization: An in situ study

IF 2.2 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2025-02-03 DOI: 10.1016/j.archoralbio.2025.106187

Leonardo Libardi Pagotto, Jaime Aparecido Cury, Antonio Pedro Ricomini-Filho

Objectives

This study investigated the effect of chlorhexidine (CHX) on demineralization, cell viability and matrix composition in EPS-rich (EPS⁺) and poor (EPS^-) oral biofilms.

Design

A split-mouth and crossover in situ study was conducted with six participants over two phases of 14 days each. During a lead-in step, biofilms were formed under either sucrose exposure (EPS⁺) or glucose + fructose exposure (EPS^-). In the experimental step, participants rinsed with either 0.9 % NaCl (negative control) or 0.12 % CHX for seven days.

Results

Data showed that EPS⁺ biofilms exhibited higher enamel surface hardness loss (%SHL), lesion area (∆S) and concentrations of soluble and insoluble EPS, compared to EPS^- biofilms. After treatments, CHX significantly reduced the counts of colony-forming units (CFU) in both EPS^- and EPS⁺ biofilms. However, CHX only reduced ∆S within the EPS^- group. There was no significant difference in demineralization change (%∆S) among treatments. Additionally, CHX decreased soluble and insoluble EPS concentrations in the EPS⁺ group.

Conclusion

While CHX effectively reduced bacterial counts, its antimicrobial effect may not be enough to reduce the enamel demineralization caused by mature EPS-rich biofilms.

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引用次数: 0

Multi-strain probiotic formula modulates expression of β-defensin-2, β-defensin-3, and TLR-4 in male rats with apical periodontitis 多菌种益生菌配方调节雄性根尖牙周炎大鼠β-防御素-2、β-防御素-3和TLR-4的表达。

IF 2.2 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2025-02-01 DOI: 10.1016/j.archoralbio.2024.106137

Leopoldo Cosme-Silva , Renan Dal-Fabbro , Fernanda de Lima Pontes , Leticia Cabrera Capalbo , Edilson Ervolino , Luciano Tavares Angelo Cintra , Juan José Segura-Egea , João Eduardo Gomes-Filho

Objective

Evaluate whether a multi-strain probiotic formula affects blood parameters (hematologic, calcium, and phosphorus levels) and alters the expression of β-defensin-2, β-defensin-3, and toll-like receptor 4 in male rats with induced apical periodontitis (AP).

Design

Wistar rats were divided into two groups (n = 8 each): (1) rats with AP on a regular diet (Control) and (2) rats with AP on a regular diet supplemented with the multi-strain probiotic GNC Probiotic Complex (GCP) at one billion CFU. AP was induced by exposing the dental pulp of the first molars to the oral environment. GCP was administered daily via gavage for 30 days during AP development. After 30 days, animals were anesthetized, a cardiac puncture was performed, and 5 mL of blood was collected for hematologic, calcium, and phosphorus analysis. Animals were then euthanized, and mandibles were removed for histological and immunochemical analysis of β-defensin-2, β-defensin-3, and toll-like receptor 4. Statistical analyses used Mann–Whitney U and Student’s t-tests, with significance at P < 0.05.

Results

No significant differences were observed in blood parameters between the Control and GCP groups (P > 0.05). In AP, the Control group showed more intense inflammatory infiltrates and higher median severity scores than the GCP group (P < 0.05). Immunoreactivity levels for β-defensin-2, β-defensin-3, and toll-like receptor 4 were significantly increased in the GCP group (P < 0.05).

Conclusion

Probiotic complex reduces inflammation and enhances immunolabeling of β-defensin-2, β-defensin-3, and toll-like receptor 4 in AP.

目的：评价多菌种益生菌配方是否影响雄性根尖牙周炎（AP）大鼠血液参数（血液学、钙、磷水平）及β-防御素-2、β-防御素-3和toll样受体4的表达。设计：Wistar大鼠分为两组（n = ，每组8只）：(1)常规饲粮中添加AP大鼠（对照组）；(2)常规饲粮中添加10亿CFU的多菌种益生菌GNC益生菌复合物（GCP）。将第一磨牙牙髓暴露于口腔环境中诱发AP。在AP发展期间，每天灌胃给予GCP，持续30天。30 d后，麻醉动物，穿刺心脏，取5 mL血液进行血液学、钙、磷分析。然后对动物实施安乐死，取下颌骨进行β-防御素-2、β-防御素-3和toll样受体4的组织学和免疫化学分析。统计分析采用Mann-Whitney U检验和Student’st检验，P值为 。结果：对照组和GCP组之间的血液参数无显著差异（P > 0.05）。在AP中，对照组比GCP组表现出更强烈的炎症浸润和更高的中位严重程度评分（P ）结论：益生菌复合物可以减轻炎症，增强AP中β-防御素-2、β-防御素-3和toll样受体4的免疫标记。

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引用次数: 0

Truncated recombinant Jagged1 fused with human IgG1 Fc activates Notch target genes in human periodontal ligament cells 截断重组Jagged1与人IgG1 Fc融合激活人牙周韧带细胞Notch靶基因。

IF 2.2 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2025-02-01 DOI: 10.1016/j.archoralbio.2024.106138

Y Nhu Tran , Ajjima Chansaenroj , Araya Jivaphetthai , Thanaphum Osathanon , Wanatchaporn Arunmanee

Objective

Jagged1, a Notch ligand, is essential for osteogenic differentiation in human periodontal ligament cells (hPDLs) by interacting with Notch2 to induce osteogenic markers, alkaline phosphatase activity, and mineral deposition. However, its large size hampers absorption and distribution of biomaterials. This study aimed to identify the critical region of Jagged1 necessary for its interaction with Notch2 to create a truncated version that retains osteogenic activity but with improved delivery characteristics.

Methods

Truncated versions of Jagged1 were designed by deleting C-terminal regions, focusing on the importance of the N-terminal domain. Both truncated and full-length Jagged1 were fused with human IgG1 Fc (Jagged1-Fc) and expressed in Chinese hamster ovary cells. hPDLs treated with these constructs were analyzed for Notch target gene expression using real-time PCR. Mineral deposition was assessed using alizarin red S staining.

Results

Both truncated and full-length Jagged1-Fc increased the expression of Notch target genes (Hes1, Hey1, and ALP) in hPDLs, indicating successful activation of Notch signaling. However, only the full-length Jagged1-Fc enhanced mineral deposition, while the truncated version did not.

Conclusions

Full-length Jagged1-Fc is required for mineral deposition and complete osteogenic differentiation in hPDLs. The truncated versions, while capable of activating Notch signaling, are ineffective in promoting mineralization, underscoring the importance of the entire protein for clinical applications in bone regeneration.

目的：Notch配体Jagged1通过与Notch2相互作用诱导成骨标志物、碱性磷酸酶活性和矿物沉积，对人牙周韧带细胞（hpdl）的成骨分化至关重要。然而，它的大尺寸阻碍了生物材料的吸收和分布。本研究旨在确定Jagged1与Notch2相互作用所必需的关键区域，以创建一个保留成骨活性但具有改进的传递特性的截断版本。方法：通过删除c端区域设计Jagged1的截断版本，重点关注n端结构域的重要性。截断和全长Jagged1均与人IgG1 Fc （Jagged1-Fc）融合，并在中国仓鼠卵巢细胞中表达。用real-time PCR分析这些构建体处理的hpdl的Notch靶基因表达情况。用茜素红S染色评价矿物沉积。结果：截断和全长Jagged1-Fc均增加了Notch靶基因Hes1、Hey1和ALP在hpdl中的表达，表明Notch信号成功激活。然而，只有全长Jagged1-Fc增强了矿物沉积，而截断版本则没有。结论：全长Jagged1-Fc对于hpdl的矿物沉积和完整的成骨分化是必需的。截断的版本，虽然能够激活Notch信号，但在促进矿化方面是无效的，强调了整个蛋白质在骨再生临床应用中的重要性。

{"title":"Truncated recombinant Jagged1 fused with human IgG1 Fc activates Notch target genes in human periodontal ligament cells","authors":"Y Nhu Tran , Ajjima Chansaenroj , Araya Jivaphetthai , Thanaphum Osathanon , Wanatchaporn Arunmanee","doi":"10.1016/j.archoralbio.2024.106138","DOIUrl":"10.1016/j.archoralbio.2024.106138","url":null,"abstract":"<div><h3>Objective</h3><div>Jagged1, a Notch ligand, is essential for osteogenic differentiation in human periodontal ligament cells (hPDLs) by interacting with Notch2 to induce osteogenic markers, alkaline phosphatase activity, and mineral deposition. However, its large size hampers absorption and distribution of biomaterials. This study aimed to identify the critical region of Jagged1 necessary for its interaction with Notch2 to create a truncated version that retains osteogenic activity but with improved delivery characteristics.</div></div><div><h3>Methods</h3><div>Truncated versions of Jagged1 were designed by deleting C-terminal regions, focusing on the importance of the N-terminal domain. Both truncated and full-length Jagged1 were fused with human IgG1 Fc (Jagged1-Fc) and expressed in Chinese hamster ovary cells. hPDLs treated with these constructs were analyzed for Notch target gene expression using real-time PCR. Mineral deposition was assessed using alizarin red S staining.</div></div><div><h3>Results</h3><div>Both truncated and full-length Jagged1-Fc increased the expression of Notch target genes (<em>Hes1, Hey1</em>, and <em>ALP</em>) in hPDLs, indicating successful activation of Notch signaling. However, only the full-length Jagged1-Fc enhanced mineral deposition, while the truncated version did not.</div></div><div><h3>Conclusions</h3><div>Full-length Jagged1-Fc is required for mineral deposition and complete osteogenic differentiation in hPDLs. The truncated versions, while capable of activating Notch signaling, are ineffective in promoting mineralization, underscoring the importance of the entire protein for clinical applications in bone regeneration.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"170 ","pages":"Article 106138"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142792931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The influence of microorganisms on bone homeostasis in apical periodontitis 微生物对根尖牙周炎患者骨稳态的影响。

IF 2.2 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2025-02-01 DOI: 10.1016/j.archoralbio.2024.106153

Dan Pan , Yu Hao , Yuyan Tao , Bolei Li , Lei Cheng

Objective

This review aims to provide an overview of the role of microorganisms in the onset and progression of periapical diseases, particularly regarding their effects on bone homeostasis.

Design

The search for this narrative review was conducted in PubMed, Web of Science and Google Scholar using relevant keywords, including checking reference lists of journal articles by hand searching.

Results

Microorganisms directly promote osteoclasts through pathways such as nuclear factor-κB (NF-κB) and extracellular regulated protein kinases (ERK), while inhibiting osteoblasts function by interfering with the wingless-related integration site (Wnt)/β-catenin pathway in the periapical area. Moreover, microorganisms indirectly regulate periapical bone homeostasis by inducing programmed cell death and modulating the immune microenvironment through the activation of innate immunity via pattern-recognition receptors (PRRs) and subsequent cascades of responses. Among these microorganisms, Enterococcus faecalis, Porphyromonas gingivalis and Fusobacterium nucleatum play significant roles.

Conclusion

Microorganisms regulate pathways such as NF-ĸB and Wnt/β-catenin, as well as programmed cell death and the immune microenvironment in the periapical area, thereby disrupting bone homeostasis.

目的：本综述旨在概述微生物在根尖周围疾病的发生和发展中的作用，特别是它们对骨稳态的影响。设计：使用相关关键词在PubMed、Web of Science和b谷歌Scholar中检索本叙述性综述，包括手工检索期刊文章的参考文献列表。结果：微生物通过核因子-κB （NF-κB）和细胞外调节蛋白激酶（ERK）等通路直接促进破骨细胞，同时通过干扰根尖周围区域的无翼相关整合位点(Wnt)/β-catenin通路抑制成骨细胞功能。此外，微生物通过模式识别受体（PRRs）和随后的级联反应激活先天免疫，通过诱导程序性细胞死亡和调节免疫微环境，间接调节根尖周骨稳态。在这些微生物中，粪肠球菌、牙龈卟啉单胞菌和核梭杆菌起着重要的作用。结论：微生物通过调控NF-ĸB和Wnt/β-catenin等通路，以及细胞程序性死亡和根尖周围免疫微环境，从而破坏骨稳态。

{"title":"The influence of microorganisms on bone homeostasis in apical periodontitis","authors":"Dan Pan , Yu Hao , Yuyan Tao , Bolei Li , Lei Cheng","doi":"10.1016/j.archoralbio.2024.106153","DOIUrl":"10.1016/j.archoralbio.2024.106153","url":null,"abstract":"<div><h3>Objective</h3><div>This review aims to provide an overview of the role of microorganisms in the onset and progression of periapical diseases, particularly regarding their effects on bone homeostasis.</div></div><div><h3>Design</h3><div>The search for this narrative review was conducted in PubMed, Web of Science and Google Scholar using relevant keywords, including checking reference lists of journal articles by hand searching.</div></div><div><h3>Results</h3><div>Microorganisms directly promote osteoclasts through pathways such as nuclear factor-κB (NF-κB) and extracellular regulated protein kinases (ERK), while inhibiting osteoblasts function by interfering with the wingless-related integration site (Wnt)/β-catenin pathway in the periapical area. Moreover, microorganisms indirectly regulate periapical bone homeostasis by inducing programmed cell death and modulating the immune microenvironment through the activation of innate immunity via pattern-recognition receptors (PRRs) and subsequent cascades of responses. Among these microorganisms, <em>Enterococcus faecalis</em>, <em>Porphyromonas gingivalis</em> and <em>Fusobacterium nucleatum</em> play significant roles.</div></div><div><h3>Conclusion</h3><div>Microorganisms regulate pathways such as NF-ĸB and Wnt/β-catenin, as well as programmed cell death and the immune microenvironment in the periapical area, thereby disrupting bone homeostasis.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"170 ","pages":"Article 106153"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142792929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

IF 2.2 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2025-02-01 DOI: 10.1016/j.archoralbio.2024.106149

Yong Liu , Jiaying Nie , Ying Huang , Yunyan Yang , Wenen Su , Yumei Zhang , Zhuoqiao Gao , Shaohui Deng , Meilin Li , Shaoyan Lian , Jieying Li , Chaoqun Liu

Objective

N6-methyladenosine (m6A) RNA dysregulation is crucial for cancer development. The study aimed to explore the effects of m6A modification in oral squamous cell carcinoma (OSCC) and its potential as a biomarker and therapeutic target.

Design

We first analyzed m6A-related gene expression and its impact on OSCC prognosis and progression using the TCGA database. Subsequently, a Chinese cohort of 134 samples was used for validation. Bioinformatics analysis was conducted with TCGA data, and m6A levels were measured in the validation cohort using a quantification kit. Survival analysis was performed to study the relationship between m6A-related genes and OSCC prognosis in the Chinese population. The expression of m6A-related genes was assessed by using quantitative real-time PCR, Western blot analysis, and immunohistochemistry.

Results

In the TCGA database, we found dysregulated expressions of METTL14, ALKBH5, YTHDF2, HNRNPC, LRPPRC, HNRNPA2B1, IGF2BP2, and RBMX in OSCC. Based on this, we observed significantly elevated total m6A content in OSCC tissues compared to normal controls in the validation cohort. Among the m6A candidate genes, only ALKBH5 and RBMX upregulation were found to be independent prognostic risk factors for poor OSCC survival in the Chinese population. And the inclusion of these two genes had a higher area under the curve for 3-year (0.705, 0.826), and 5-year (0.715, 0.788) overall survival compared to the model that only considered clinical parameters.

Conclusions

We found the upregulation of m6A status in OSCC, of which, ALKBH5 and RBMX may serve as promising diagnostic and prognostic biomarkers for Chinese patients with OSCC.

目的：n6 -甲基腺苷（n6 - methylladenosine, m6A） RNA异常在肿瘤发生发展中起着至关重要的作用。本研究旨在探讨m6A修饰在口腔鳞状细胞癌（OSCC）中的作用及其作为生物标志物和治疗靶点的潜力。设计：我们首先使用TCGA数据库分析m6a相关基因表达及其对OSCC预后和进展的影响。随后，使用中国134个样本队列进行验证。使用TCGA数据进行生物信息学分析，并使用定量试剂盒测量验证队列中的m6A水平。通过生存分析研究中国人群中m6a相关基因与OSCC预后的关系。采用实时荧光定量PCR、Western blot和免疫组织化学检测m6a相关基因的表达。结果：在TCGA数据库中，我们发现metttl14、ALKBH5、YTHDF2、HNRNPC、LRPPRC、HNRNPA2B1、IGF2BP2、RBMX在OSCC中表达异常。基于此，我们观察到在验证队列中，与正常对照相比，OSCC组织中总m6A含量显著升高。在m6A候选基因中，只有ALKBH5和RBMX上调被发现是中国人群OSCC生存不良的独立预后危险因素。与仅考虑临床参数的模型相比，纳入这两个基因的3年（0.705,0.826）和5年（0.715,0.788）总生存率曲线下面积更高。结论：我们发现在OSCC中m6A水平上调，其中ALKBH5和RBMX可能作为中国OSCC患者有希望的诊断和预后生物标志物。

{"title":"m6A-related genes ALKBH5 and RBMX as prognostic and progression biomarkers in Chinese oral squamous cell carcinoma patients","authors":"Yong Liu , Jiaying Nie , Ying Huang , Yunyan Yang , Wenen Su , Yumei Zhang , Zhuoqiao Gao , Shaohui Deng , Meilin Li , Shaoyan Lian , Jieying Li , Chaoqun Liu","doi":"10.1016/j.archoralbio.2024.106149","DOIUrl":"10.1016/j.archoralbio.2024.106149","url":null,"abstract":"<div><h3>Objective</h3><div>N6-methyladenosine (m6A) RNA dysregulation is crucial for cancer development. The study aimed to explore the effects of m6A modification in oral squamous cell carcinoma (OSCC) and its potential as a biomarker and therapeutic target.</div></div><div><h3>Design</h3><div>We first analyzed m6A-related gene expression and its impact on OSCC prognosis and progression using the TCGA database. Subsequently, a Chinese cohort of 134 samples was used for validation. Bioinformatics analysis was conducted with TCGA data, and m6A levels were measured in the validation cohort using a quantification kit. Survival analysis was performed to study the relationship between m6A-related genes and OSCC prognosis in the Chinese population. The expression of m6A-related genes was assessed by using quantitative real-time PCR, Western blot analysis, and immunohistochemistry.</div></div><div><h3>Results</h3><div>In the TCGA database, we found dysregulated expressions of METTL14, ALKBH5, YTHDF2, HNRNPC, LRPPRC, HNRNPA2B1, IGF2BP2, and RBMX in OSCC. Based on this, we observed significantly elevated total m6A content in OSCC tissues compared to normal controls in the validation cohort. Among the m6A candidate genes, only ALKBH5 and RBMX upregulation were found to be independent prognostic risk factors for poor OSCC survival in the Chinese population. And the inclusion of these two genes had a higher area under the curve for 3-year (0.705, 0.826), and 5-year (0.715, 0.788) overall survival compared to the model that only considered clinical parameters.</div></div><div><h3>Conclusions</h3><div>We found the upregulation of m6A status in OSCC, of which, ALKBH5 and RBMX may serve as promising diagnostic and prognostic biomarkers for Chinese patients with OSCC.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"170 ","pages":"Article 106149"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142792882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro development of a radicular cyst: A morphological investigation of a spheroid cyst-like model associated with fibroblasts

IF 2.2 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2025-02-01 DOI: 10.1016/j.archoralbio.2025.106186

D. Nascimento , LM Brand , L. Bernardi , JM Vasconcelos , IC Guedes , Figueiredo JAP , F. Visioli , ML Lamers , PV Rados

Objective

The aim of this study was to improve the in vitro model of tooth radicular cyst previously developed by incorporating stromal components and to describe histologic analysis.

Design

A radicular cystogenesis-like 3D model was generated using HaCaT cells (1 × 10⁵) to developing spheroid. After 24 h, spheroids were embedded in non-polymerized collagen in combination with 1 × 10⁵ fibroblast cells (HaCaT + 1 × 10⁵ hFIB) to mimic stromal microenvironment. Micrographs were taken to evaluate the cystic stability and dispersion area, while histological hematoxylin/eosin staining was used to measure the ratio of epithelial lining area. Analysis was conducted on days 1, 3, and 7 using ImageJ software. Statistical analyses were performed using GraphPad Prism 5 software.

Results

The model, with fibroblasts included, preserved the cystic structure and allowed cyst growth, with an increase in both the area and dispersion of the cystic structure throughout the experimental period (p < 0.05). Histological analysis of the cyst model revealed morphological similarities with in vivo tooth radicular cyst biopsies, showing a cystic cavity lined by an epithelial layer, surrounded by collagen and fibroblasts. Additionally, the cavity area increased while the limiting epithelial area decreased. The highest epithelial area-to-total area ratio was observed in day 1 spheroids, while the lowest was found on day 7 (p < 0.05).

Conclusion

The incorporation of fibroblasts improved the in vitro cystogenesis model, since it did not interfere with the model’s development and more closely mimicked the in vivo microenvironment.

{"title":"In vitro development of a radicular cyst: A morphological investigation of a spheroid cyst-like model associated with fibroblasts","authors":"D. Nascimento , LM Brand , L. Bernardi , JM Vasconcelos , IC Guedes , Figueiredo JAP , F. Visioli , ML Lamers , PV Rados","doi":"10.1016/j.archoralbio.2025.106186","DOIUrl":"10.1016/j.archoralbio.2025.106186","url":null,"abstract":"<div><h3>Objective</h3><div>The aim of this study was to improve the <em>in vitro</em> model of tooth radicular cyst previously developed by incorporating stromal components and to describe histologic analysis.</div></div><div><h3>Design</h3><div>A radicular cystogenesis-like 3D model was generated using HaCaT cells (1 × 10<sup>5</sup>) to developing spheroid. After 24 h, spheroids were embedded in non-polymerized collagen in combination with 1 × 10<sup>5</sup> fibroblast cells (HaCaT + 1 × 10<sup>5</sup> hFIB) to mimic stromal microenvironment. Micrographs were taken to evaluate the cystic stability and dispersion area, while histological hematoxylin/eosin staining was used to measure the ratio of epithelial lining area. Analysis was conducted on days 1, 3, and 7 using ImageJ software. Statistical analyses were performed using GraphPad Prism 5 software.</div></div><div><h3>Results</h3><div>The model, with fibroblasts included, preserved the cystic structure and allowed cyst growth, with an increase in both the area and dispersion of the cystic structure throughout the experimental period (p < 0.05). Histological analysis of the cyst model revealed morphological similarities with <em>in vivo</em> tooth radicular cyst biopsies, showing a cystic cavity lined by an epithelial layer, surrounded by collagen and fibroblasts. Additionally, the cavity area increased while the limiting epithelial area decreased. The highest epithelial area-to-total area ratio was observed in day 1 spheroids, while the lowest was found on day 7 (p < 0.05).</div></div><div><h3>Conclusion</h3><div>The incorporation of fibroblasts improved the <em>in vitro</em> cystogenesis model, since it did not interfere with the model’s development and more closely mimicked the <em>in vivo</em> microenvironment.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"172 ","pages":"Article 106186"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143153944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phospho-cofilin predicts efficiency of Fasudil for oral squamous cell carcinoma treatment through Yes-associated protein inhibition

IF 2.2 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2025-01-29 DOI: 10.1016/j.archoralbio.2025.106185

Ying Wu , Yuchen Jiang , Lanxin Jiang , Yang Peng , Tong Zhou , Xiaoqiang Xia , Feifei Hou , Qiuyun Yuan , Lu Ye , Weideng Wei , Jiuge Zhang , Qianming Chen , Xiaodong Feng

Objectives

This study evaluates Fasudil, a Rho-associated coiled-coil-containing protein kinase (ROCK) inhibitor, for its potential to inhibit oral squamous cell carcinoma (OSCC) growth and explores phospho-cofilin as a potential biomarker for prediction treatment efficiency of Fasudil in OSCC.

Design

A cohort of 109 OSCC patients provided tissue samples for phospho-cofilin expression analysis and survival analysis. The study examined the effect of Fasudil on OSCC cell lines HSC-3, UM1, and CAL33, assessing tumor growth inhibition through various in vitro and in vivo experiments. ROCK inhibition response and downstream mechanisms were explored by RNA sequencing, q-PCR, and immunofluorescence.

Results

High phospho-cofilin expression in OSCC tissues correlated with poor patient outcomes and was a reliable biomarker for ROCK activity. Fasudil inhibited growth in OSCC cell lines, particularly those with high phospho-cofilin expression. ROCK inhibition led to downregulation of Yes-associated protein (YAP) activity, resulting in suppressed tumor proliferation and increased apoptosis both in vitro and in vivo.

Conclusions

Inhibition of ROCK/phospho-cofilin/YAP by Fasudil could suppress OSCC proliferation, while phospho-cofilin served as a potential biomarker of OSCC.

{"title":"Phospho-cofilin predicts efficiency of Fasudil for oral squamous cell carcinoma treatment through Yes-associated protein inhibition","authors":"Ying Wu , Yuchen Jiang , Lanxin Jiang , Yang Peng , Tong Zhou , Xiaoqiang Xia , Feifei Hou , Qiuyun Yuan , Lu Ye , Weideng Wei , Jiuge Zhang , Qianming Chen , Xiaodong Feng","doi":"10.1016/j.archoralbio.2025.106185","DOIUrl":"10.1016/j.archoralbio.2025.106185","url":null,"abstract":"<div><h3>Objectives</h3><div>This study evaluates Fasudil, a Rho-associated coiled-coil-containing protein kinase (ROCK) inhibitor, for its potential to inhibit oral squamous cell carcinoma (OSCC) growth and explores phospho-cofilin as a potential biomarker for prediction treatment efficiency of Fasudil in OSCC.</div></div><div><h3>Design</h3><div>A cohort of 109 OSCC patients provided tissue samples for phospho-cofilin expression analysis and survival analysis. The study examined the effect of Fasudil on OSCC cell lines HSC-3, UM1, and CAL33, assessing tumor growth inhibition through various in vitro and in vivo experiments. ROCK inhibition response and downstream mechanisms were explored by RNA sequencing, q-PCR, and immunofluorescence.</div></div><div><h3>Results</h3><div>High phospho-cofilin expression in OSCC tissues correlated with poor patient outcomes and was a reliable biomarker for ROCK activity. Fasudil inhibited growth in OSCC cell lines, particularly those with high phospho-cofilin expression. ROCK inhibition led to downregulation of Yes-associated protein (YAP) activity, resulting in suppressed tumor proliferation and increased apoptosis both in vitro and in vivo.</div></div><div><h3>Conclusions</h3><div>Inhibition of ROCK/phospho-cofilin/YAP by Fasudil could suppress OSCC proliferation, while phospho-cofilin served as a potential biomarker of OSCC.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"172 ","pages":"Article 106185"},"PeriodicalIF":2.2,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143082616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0