Archives of oral biology最新文献_第2页

The influence of simvastatin on osteoblast functionality in the presence of titanium dioxide particles In-vitro 辛伐他汀在二氧化钛颗粒存在下对成骨细胞功能的影响体外试验

IF 2.2 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2024-08-06 DOI: 10.1016/j.archoralbio.2024.106065

Objective

Leaching of particles from dental titanium implant surfaces into preimplant microenvironment causes detrimental effects on bone cells. The current study investigated influence of simvastatin in mitigating adverse pro-inflammatory effects of titanium dioxide (TiO₂) micro (MP) and nano (NP) particles on hFOB 1.19 cells in vitro.

Design

Viability of hFOB 1.19 cells following exposure to varying concentrations of TiO₂ MPs and NPs and simvastatin were measured by XTT assay. hFOB 1.19 cells were treated with 100 µg/mL of TiO₂ MPs, 100 µg/mL of TiO₂ NPs, 0.1 µM simvastatin, 100 µg/mL of TiO₂ MPs+ 0.1 µM simvastatin and 100 µg/mL of TiO₂ NPs+ 0.1 µM simvastatin. After 24 h, ROS was measured by flow cytometry. On day 14, real-time PCR analysis for pro-inflammatory cytokines and bone formation markers was done for TNFα, IL1β, osteocalcin, ALP, and Col1 markers; while ALP and RANKL/OPG ratio were determined by colorimetric and ELISA assays respectively. Further, mineralization study using Alizarin Red S staining (ARS) and calcium quantification were performed.

Results

Exposure of hFOB to TiO₂ MPs and NPs generated ROS and reduced cell viability significantly, with upregulation of pro-inflammatory markers TNFα and IL1β and downregulation of bone formation markers OC and increased RANKL/OPG ratio and lowered degree of mineralization. Treatment with 0.1 µM of simvastatin treatment reversed the effects by mitigating oxidative stress, dampening pro-inflammatory markers, upregulation of bone formation markers, lowering RANKL/OPG ratio and increasing degree of mineralization.

Conclusion

Simvastatin possesses antioxidant, anti-inflammatory, and pro-osteogenic properties that may support bone healing around titanium implants.

目的牙科钛种植体表面的颗粒浸出到种植体前的微环境中会对骨细胞产生有害影响。本研究调查了辛伐他汀在减轻二氧化钛（TiO2）微米（MP）和纳米（NP）颗粒对体外 hFOB 1.19 细胞的不良促炎作用方面的影响。hFOB 1.19细胞分别接受100微克/毫升TiO2 MPs、100微克/毫升TiO2 NPs、0.1微摩尔辛伐他汀、100微克/毫升TiO2 MPs+ 0.1微摩尔辛伐他汀和100微克/毫升TiO2 NPs+ 0.1微摩尔辛伐他汀的处理。24 小时后，用流式细胞仪测量 ROS。第 14 天，对 TNFα、IL1β、骨钙素、ALP 和 Col1 标记的促炎细胞因子和骨形成标记进行了实时 PCR 分析；ALP 和 RANKL/OPG 比率分别通过比色法和酶联免疫吸附法测定。结果将 hFOB 暴露于 TiO2 MPs 和 NPs 会产生 ROS 并显著降低细胞活力，上调促炎标志物 TNFα 和 IL1β，下调骨形成标志物 OC，增加 RANKL/OPG 比率并降低矿化度。使用 0.1 µM 辛伐他汀治疗可通过减轻氧化应激、抑制促炎标志物、上调骨形成标志物、降低 RANKL/OPG 比率和提高矿化度来逆转上述影响。

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引用次数: 0

Diagnostic significance of LncRNA MIAT in periodontitis and the molecular mechanisms influencing periodontal ligament fibroblasts via the miR-204-5p/DKK1 axis LncRNA MIAT 在牙周炎中的诊断意义以及通过 miR-204-5p/DKK1 轴影响牙周韧带成纤维细胞的分子机制

IF 2.2 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2024-08-04 DOI: 10.1016/j.archoralbio.2024.106066

Objective

This study investigated the clinical importance of long noncoding RNA myocardial infarction-associated transcript (MIAT) in periodontitis and its impact on the functional regulation of human periodontal ligament fibroblasts (hPDLFs).

Methods

Ninety-eight periodontitis patients and 74 healthy controls were enrolled. In vitro cellular models were created using Porphyromonas gingivalis lipopolysaccharide (Pg-LPS) to stimulate hPDLFs. Real-time quantitative polymerase chain reaction was used to measure mRNA levels of MIAT and osteogenic factors. Inflammation factor concentration was assessed using an enzyme-linked immunosorbent assay. Cell viability and apoptosis were examined by cell counting kit −8 and flow cytometry assay. The targeting relationship was verified by the dual-luciferase reporter and RNA Immunoprecipitation assay.

Results

Highly expressed MIAT and Dicckopf-1 (DDK1), and lowly expressed miR-204–5p were found in the gingival crevicular fluid of periodontitis patients and Pg-LPS induced hPDLFs. MIAT has a sensitivity of 76.53 % and a specificity of 86.49 % for identifying patients with periodontitis among healthy individuals. MIAT acts as a sponge for miR-204–5p and upregulates DDK1 mRNA expression. Silencing of MIAT diminished the promotion of apoptosis and inflammation in hPDLFs by Pg-LPS and enhanced osteogenic differentiation. However, a miR-204–5p inhibitor significantly reversed the effect of silenced MIAT.

Conclusions

MIAT may act as a promising biomarker for periodontitis. It modulates apoptosis, inflammation, and osteogenic differentiation of PDLFs by focusing on the miR-204–5p/DKK1 axis, indicating its potential as a new therapeutic target for treating periodontitis.

本研究探讨了长非编码 RNA 心肌梗死相关转录本（MIAT）在牙周炎中的临床重要性及其对人牙周韧带成纤维细胞（hPDLFs）功能调控的影响。使用牙龈卟啉单胞菌脂多糖（Pg-LPS）刺激 hPDLFs，建立体外细胞模型。实时定量聚合酶链反应用于测量 MIAT 和成骨因子的 mRNA 水平。使用酶联免疫吸附试验评估炎症因子的浓度。通过细胞计数试剂盒-8 和流式细胞术检测细胞活力和凋亡。结果在牙周炎患者和 Pg-LPS 诱导的 hPDLFs 的牙龈沟液中发现了高表达的 MIAT 和 Dicckopf-1 (DDK1)，以及低表达的 miR-204-5p。MIAT 对从健康人中识别牙周炎患者的灵敏度为 76.53%，特异度为 86.49%。MIAT 是 miR-204-5p 的海绵，能上调 DDK1 mRNA 的表达。沉默 MIAT 可减少 Pg-LPS 对 hPDLFs 细胞凋亡和炎症的促进作用，并增强成骨分化。然而，miR-204-5p抑制剂能明显逆转沉默MIAT的效果。它通过聚焦于 miR-204-5p/DKK1 轴来调节 PDLFs 的凋亡、炎症和成骨分化，表明它有可能成为治疗牙周炎的新靶点。

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引用次数: 0

Antimicrobial effects of epigallocatechin-3-gallate, a catechin abundant in green tea, on periodontal disease-associated bacteria 绿茶中富含的儿茶素表没食子儿茶素-3-棓酸盐对牙周病相关细菌的抗菌作用。

IF 2.2 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2024-07-31 DOI: 10.1016/j.archoralbio.2024.106063

Objective

Epigallocatechin-3-gallate (EGCG), a catechin abundant in green tea, exhibits antibacterial activity. In this study, the antimicrobial effects of EGCG on periodontal disease-associated bacteria (Porphyromonas gingivalis, Prevotella intermedia, Prevotella nigrescens, Fusobacterium nucleatum, and Fusobacterium periodontium) were evaluated and compared with its effects on Streptococcus mutans, a caries-associated bacterium.

Results

Treatment with 2 mg/ml EGCG for 4 h killed all periodontal disease-associated bacteria, whereas it only reduced the viable count of S. mutans by about 40 %. Regarding growth, the periodontal disease-associated bacteria were more susceptible to EGCG than S. mutans, based on the growth inhibition ring test. As for metabolism, the 50 % inhibitory concentration (IC₅₀) of EGCG for bacterial metabolic activity was lower for periodontal disease-associated bacteria (0.32–0.65 mg/ml) than for S. mutans (1.14 mg/ml). Furthermore, these IC₅₀ values were negatively correlated with the growth inhibition ring (r = −0.73 to −0.86). EGCG induced bacterial aggregation at the following concentrations: P. gingivalis (>0.125 mg/ml), F. periodonticum (>0.5 mg/ml), F. nucleatum (>1 mg/ml), and P. nigrescens (>2 mg/ml). S. mutans aggregated at an EGCG concentration of > 1 mg/ml.

Conclusion

EGCG may help to prevent periodontal disease by killing bacteria, inhibiting bacterial growth by suppressing bacterial metabolic activity, and removing bacteria through aggregation.

目的：表没食子儿茶素-3-棓酸盐（EGCG）是绿茶中富含的一种儿茶素，具有抗菌活性。本研究评估了 EGCG 对牙周病相关细菌（牙龈卟啉单胞菌、中间前驱菌、黑前驱菌、核分枝杆菌和牙周分枝杆菌）的抗菌作用，并将其与对龋齿相关细菌变异链球菌的作用进行了比较：结果：用 2 毫克/毫升的 EGCG 处理 4 小时，可杀死所有牙周病相关细菌，但只能将变异链球菌的存活数量减少约 40%。在生长方面，根据生长抑制环试验，牙周病相关细菌对 EGCG 的敏感性高于变异杆菌。在新陈代谢方面，EGCG 对细菌新陈代谢活动的 50% 抑制浓度（IC50）对牙周病相关细菌（0.32-0.65 毫克/毫升）低于对变异杆菌（1.14 毫克/毫升）。此外，这些 IC50 值与生长抑制环呈负相关（r = -0.73 至 -0.86）。在以下浓度下，EGCG 可诱导细菌聚集：牙龈P.（>0.125 mg/ml）、牙周F.（>0.5 mg/ml）、F.nucleatum（>1 mg/ml）和P.nigrescens（>2 mg/ml）。当 EGCG 浓度大于 1 毫克/毫升时，S. mutans 会聚集：结论：EGCG 可杀死细菌，通过抑制细菌的代谢活动抑制细菌生长，并通过聚集作用清除细菌，从而有助于预防牙周病。

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引用次数: 0

A persistent mineralization process in alveolar bone throughout the postnatal growth stage in rats 大鼠牙槽骨在整个出生后生长阶段的持续矿化过程。

IF 2.2 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2024-07-30 DOI: 10.1016/j.archoralbio.2024.106062

Objective

Alveolar bone quality is essential for the maxillofacial integrity and function, and depends on alveolar bone mineralization. This study aims to investigate the in vivo changes in alveolar bone mineralization, from the perspective of mineral deposition and crystal transition in postnatal rats.

Design

Nine postnatal time points of Wistar rats, ranging from day 1 to 56, were set to obtain the maxillary alveolar bone samples. Each time point consisted of ninety rats, with 45 females and 45 males. Macromorphology of alveolar bone was reconducted by Micro-Computed Tomography and the mineral content was quantified via Thermogravimetric analysis, Scanning Electron Microscope, High-Resolution Transmission Electron Microscopy and vibrational spectroscopy. Furthermore, the crystallinity and composition were characterized by vibrational spectroscopy, X-ray Diffraction, X-ray Photoelectron Spectroscopy and Selected Area Electron Diffraction.

Results

The progressive increase of mineral deposition was accompanied by substantial growth in alveolar bone mass and volume in postnatal rats. Whereas the mineral percentage initially decreased and then increased, reaching a nadir on postnatal day 14 (P14) when tooth eruption was first observed. Besides, localized mineralization was initiated by the formation of amorphous precursors and then converted into mineral crystals, while there was no statistically significant change in the average crystallinity of the bone during growth.

Conclusion

Mineralization of alveolar bone is ongoing throughout the early growth in postnatal rats. Mineral deposition increases with age, whereas the crystallinity remains stable within a certain range. Besides, the mineral percentage reaches its lowest point on P14, which may be attributed to tooth eruption.

目的：牙槽骨质量对颌面部的完整性和功能至关重要，并取决于牙槽骨矿化。本研究旨在从矿物质沉积和晶体转变的角度研究出生后大鼠牙槽骨矿化的体内变化：设计：从大鼠出生后第 1 天到第 56 天的 9 个时间点采集上颌骨牙槽骨样本。每个时间点包括 90 只大鼠，其中雌性 45 只，雄性 45 只。通过显微计算机断层扫描对牙槽骨的宏观形态进行了重建，并通过热重分析、扫描电子显微镜、高分辨率透射电子显微镜和振动光谱对矿物质含量进行了量化。此外，还通过振动光谱、X 射线衍射、X 射线光电子能谱和选区电子衍射对结晶度和成分进行了表征：结果：随着矿物质沉积的逐渐增加，出生后大鼠的牙槽骨质量和体积也大幅增长。而矿物质百分比最初下降，然后上升，在出生后第 14 天（P14）达到最低点，此时首次观察到牙齿萌出。此外，局部矿化是由无定形前体的形成开始的，然后转化为矿物晶体，而在生长过程中骨的平均结晶度没有显著的统计学变化：结论：在出生后大鼠的早期生长过程中，牙槽骨的矿化一直在进行。结论：出生后大鼠牙槽骨的矿化过程贯穿整个生长早期，矿物质沉积随着年龄的增长而增加，而结晶度则在一定范围内保持稳定。此外，矿物质百分比在 P14 达到最低点，这可能与牙齿萌出有关。

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引用次数: 0

Neohesperidin exerts subtle yet comprehensive regulation of mouse dental papilla cell-23 in vitro 新橙皮甙对体外小鼠牙乳头细胞-23具有微妙而全面的调节作用。

IF 2.2 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2024-07-24 DOI: 10.1016/j.archoralbio.2024.106055

Objective

The molecular regulation of odontoblasts in dentin formation remains largely uncharacterized. Using neohesperidin (NEO), a well-documented osteoblast regulator, we investigated whether and how NEO participates in odontoblast regulation through longitudinal treatments using various doses of NEO.

Design

Mouse dental papilla cell-23 (MDPC-23) served as a model for odontoblasts. MDPC-23 were treated with various doses of NEO (0, 1, 5, 10, 15, 20 μmol/L). Proliferation was assessed using the Cell counting kit-8 assay. Survival/apoptosis was assayed by live/dead ratio. Migration capability was assessed using scratch healing and Transwell migration assays. Mineralization was assessed using alkaline phosphatase staining and alizarin red staining. The expression levels of four key genes (Runx2, osteocalcin [OCN], β-catenin, and bone morphogenetic protein [BMP]−2) representing NEO-induced differentiation of MDPC-23 were measured by quantitative reverse transcription polymerase chain reaction.

Results

The proliferation trajectories of MDPC-23 treated with the five doses of NEO demonstrated similar curves, with a rapid increase in the 10 μmol/L NEO condition after 48 h of treatment. Similar dose-dependent trajectories were observed for survival/apoptosis. All four key genes representing odontogenic differentiation were upregulated in MDPC-23 induced by NEO treatments at two optimal doses (5 μmol/L and 10 μmol/L). Optimal migration and mobility trajectories were observed in MDPC-23 treated with 10 μmol/L NEO. Optimal mineralization was observed in MDPC-23 treated with 5 μmol/L NEO.

Conclusion

NEO can subtly regulate odontoblast proliferation, differentiation, migration, and mineralization in vitro. NEO at 5–10 μmol/L offers a safe and effective perspective for clinical promotion of dentin bridge formation in teenagers.

目的：牙本质形成过程中牙本质母细胞的分子调控在很大程度上仍未定性。新橙皮甙（NEO）是一种已被证实的成骨细胞调节剂，我们利用不同剂量的新橙皮甙进行纵向处理，研究了新橙皮甙是否以及如何参与牙本质母细胞的调节：设计：以小鼠牙乳头细胞-23（MDPC-23）为骨细胞模型。用不同剂量的 NEO（0、1、5、10、15、20 μmol/L）处理 MDPC-23。增殖采用细胞计数试剂盒-8测定法进行评估。存活/凋亡通过活/死比率进行评估。迁移能力采用划痕愈合和 Transwell 迁移试验进行评估。矿化度通过碱性磷酸酶染色和茜素红染色进行评估。通过定量反转录聚合酶链反应测定了代表NEO诱导MDPC-23分化的四个关键基因（Runx2、骨钙素[OCN]、β-catenin和骨形态发生蛋白[BMP]-2）的表达水平：结果：用五种剂量的NEO处理MDPC-23，其增殖轨迹表现出相似的曲线，在10 μmol/L NEO条件下，增殖在处理48小时后迅速增加。在存活/凋亡方面也观察到了类似的剂量依赖性轨迹。在两个最佳剂量（5 μmol/L 和 10 μmol/L）的 NEO 处理诱导下，MDPC-23 中代表牙体分化的所有四个关键基因均上调。在使用 10 μmol/L NEO 处理的 MDPC-23 中观察到了最佳迁移和移动轨迹。用5 μmol/L NEO处理的MDPC-23可观察到最佳矿化过程：结论：NEO能微妙地调节体外颌骨母细胞的增殖、分化、迁移和矿化。5-10 μmol/L 的 NEO 为临床促进青少年牙本质桥的形成提供了一个安全有效的视角。

{"title":"Neohesperidin exerts subtle yet comprehensive regulation of mouse dental papilla cell-23 in vitro","authors":"","doi":"10.1016/j.archoralbio.2024.106055","DOIUrl":"10.1016/j.archoralbio.2024.106055","url":null,"abstract":"<div><h3>Objective</h3><p>The molecular regulation of odontoblasts in dentin formation remains largely uncharacterized. Using neohesperidin (NEO), a well-documented osteoblast regulator, we investigated whether and how NEO participates in odontoblast regulation through longitudinal treatments using various doses of NEO.</p></div><div><h3>Design</h3><p>Mouse dental papilla cell-23 (MDPC-23) served as a model for odontoblasts. MDPC-23 were treated with various doses of NEO (0, 1, 5, 10, 15, 20 μmol/L). Proliferation was assessed using the Cell counting kit-8 assay. Survival/apoptosis was assayed by live/dead ratio. Migration capability was assessed using scratch healing and Transwell migration assays. Mineralization was assessed using alkaline phosphatase staining and alizarin red staining. The expression levels of four key genes (Runx2, osteocalcin [OCN], β-catenin, and bone morphogenetic protein [BMP]−2) representing NEO-induced differentiation of MDPC-23 were measured by quantitative reverse transcription polymerase chain reaction.</p></div><div><h3>Results</h3><p>The proliferation trajectories of MDPC-23 treated with the five doses of NEO demonstrated similar curves, with a rapid increase in the 10 μmol/L NEO condition after 48 h of treatment. Similar dose-dependent trajectories were observed for survival/apoptosis. All four key genes representing odontogenic differentiation were upregulated in MDPC-23 induced by NEO treatments at two optimal doses (5 μmol/L and 10 μmol/L). Optimal migration and mobility trajectories were observed in MDPC-23 treated with 10 μmol/L NEO. Optimal mineralization was observed in MDPC-23 treated with 5 μmol/L NEO.</p></div><div><h3>Conclusion</h3><p>NEO can subtly regulate odontoblast proliferation, differentiation, migration, and mineralization in vitro. NEO at 5–10 μmol/L offers a safe and effective perspective for clinical promotion of dentin bridge formation in teenagers.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141790289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Arecoline promotes fibroblast activation and M2-macrophage polarization by up-regulating the expression of IL-4 阿雷科林通过上调 IL-4 的表达，促进成纤维细胞活化和 M2-巨噬细胞极化。

IF 2.2 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2024-07-20 DOI: 10.1016/j.archoralbio.2024.106052

Objective

To determine the biological effects of arecoline on oral submucous fibrosis (OSF).

Design

The differential genes between OSF tissue and normal oral tissue were collected form GSE64216 dataset, analyzed by Gene Expression Omnibus (GEO) database. Real-time PCR and immunohistochemistry were used to analyze the expression of IL-4 gene and protein in oral tissue. Enzyme-linked immunosorbent assay (ELISA) was used to analyze the expression of exocrine IL-4 protein in human oral fibroblasts (HOF) pre-treated by arecoline. Cell Counting Kit-8 (CCK-8) and transwell assays were used to analyze the proliferation and migration of HOF cells, respectively. After IL-4 was knocked down by short hairpin (sh) plasmid, the proliferation and migration of HOF cells were detected. Flow cytometry was used to analyze the proportion of M2-macrophages. Real-time PCR and immunohistochemistry were used to verify the expression of biomarker proteins of macrophages in OSF tissues.

Results

The expression of IL-4 gene and protein were both up-regulated in OSF tissue. Arecoline could enhance the expression of IL-4 gene and exocrine protein in HOF cells, and promote the proliferation and migration of HOF cells. While knockdown of IL-4 could inhibit arecoline-induced proliferation and migration in HOF cells. The results of flow cytometry showed that recombinant human IL-4 (rhIL-4) protein could increase the proportion of M2-macrophages. Similarly, the results of real-time PCR and immunohistochemistry showed the expression of ARG1 (Biomarker proteins of M2-macrophage) was up-regulated in OSF tissues.

Conclusion

Arecoline promotes activation of fibroblasts and polarization of M2-macrophages by up-regulating the expression of IL-4.

目的：确定异甲唑啉对口腔黏膜下纤维化（OSF）的生物效应：确定阿斯考林对口腔黏膜下纤维化（OSF）的生物学效应：通过基因表达总库（Gene Expression Omnibus，GEO）分析，收集GSE64216数据集中OSF组织与正常口腔组织的差异基因。采用实时 PCR 和免疫组织化学方法分析口腔组织中 IL-4 基因和蛋白的表达。酶联免疫吸附试验（ELISA）用于分析经异丙嗪预处理的人口腔成纤维细胞（HOF）中外分泌型 IL-4 蛋白的表达。细胞计数试剂盒-8（CCK-8）和透孔试验分别用于分析 HOF 细胞的增殖和迁移。用短发夹质粒敲除IL-4后，检测HOF细胞的增殖和迁移。流式细胞术用于分析 M2-巨噬细胞的比例。实时荧光定量PCR技术和免疫组化技术验证了OSF组织中巨噬细胞生物标志蛋白的表达：结果：OSF组织中IL-4基因和蛋白的表达均上调。阿雷科林能增强 HOF 细胞中 IL-4 基因和外分泌蛋白的表达，促进 HOF 细胞的增殖和迁移。而IL-4基因敲除可抑制arecoline诱导的HOF细胞增殖和迁移。流式细胞术结果显示，重组人IL-4（rhIL-4）蛋白可增加M2-巨噬细胞的比例。同样，实时荧光定量PCR和免疫组化的结果表明，ARG1（M2-巨噬细胞的生物标志蛋白）在OSF组织中表达上调：结论：Arecoline通过上调IL-4的表达促进成纤维细胞的活化和M2-巨噬细胞的极化。

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引用次数: 0

A novel missense variant in CAT gene causing acatalasemia with gangrenous periodontitis (Takahara’s disease) 导致伴有坏疽性牙周炎（高原病）的苊血症的 CAT 基因新型错义变异体

IF 2.2 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2024-07-17 DOI: 10.1016/j.archoralbio.2024.106054

Objectives

Acatalasemia is a very rare disorder characterized by gangrenous oral ulcerations and is caused by biallelic variants in the CAT gene which encodes the catalase enzyme that decomposes the hydrogen peroxide molecules to remove their toxic effect. We report two siblings from a consanguineous Egyptian family presenting with joint hyperlaxity, loose dentitions with gangrenous periodontitis, and early loss of teeth.

Study design

The patients were clinically suspected to have the periodontal type of Ehlers-Danlos syndrome and thus genetic testing of C1S and C1R causative genes was carried out first by Sanger sequencing then exome sequencing (ES) was considered.

Results

No pathogenic variants were detected in C1S and C1R genes then ES revealed a new homozygous missense variant in the CAT gene segregating in the family, c .635 T > G (p.Met212Arg).

Conclusion

We describe the first Egyptian cases with acatalasemia and expand the mutational spectrum of this rare disorder. Premature loss of teeth is an emerging finding in our cases and addresses the hazardous systemic manifestations associated with the disorder. The rarity of inherited orodental diseases renders the accurate diagnosis difficult and complicates the symptoms. Therefore, the use of advanced molecular technologies is highly advisable for early diagnosis and management of patients.

目的：过氧化氢酶血症是一种非常罕见的疾病，以坏疽性口腔溃疡为特征，由 CAT 基因的双倍变体引起。我们报告了来自一个埃及近亲家庭的两对兄弟姐妹，他们表现为关节过度松弛、牙齿松动并伴有坏疽性牙周炎和早期牙齿脱落。研究设计临床上怀疑患者患有埃勒斯-丹洛斯综合征的牙周型，因此首先通过桑格测序对 C1S 和 C1R 致病基因进行了基因检测，然后考虑进行外显子组测序（ES）。结果在 C1S 和 C1R 基因中未检测到致病变异，而 ES 则发现了该家族中新出现的 CAT 基因同源错义变异，即 c .635 T > G (p.Met212Arg).在我们的病例中，牙齿过早脱落是一个新发现，并涉及到与该疾病相关的危险的全身表现。遗传性口腔疾病的罕见性使准确诊断变得困难，也使症状变得复杂。因此，利用先进的分子技术对患者进行早期诊断和治疗是非常可取的。

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引用次数: 0

Cerium- and samarium-nitrate interaction and accumulation on human dentin 硝酸铈和硝酸钐在人类牙本质上的相互作用和积累

IF 2.2 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2024-07-16 DOI: 10.1016/j.archoralbio.2024.106053

Objective

To investigate the accumulation of cerium-nitrate and samarium-nitrate on dentin without or with smear-layer and to test their antibacterial activity.

Design

24 dentin-enamel slices were cut from 24 extracted molars. 12 slices underwent smear-layer creation (320 grit, 200 g, 5 s), the other 12 smear-layer removal (20 % EDTA, 300 s). Slices were halved to 48 semilunar-shaped specimens. One specimen per tooth was treated with either Ce(NO₃)₃ (50 wt% aqueous solution; pH = 1.29; n = 6) or Sm(NO₃)₃ (50 wt% aqueous solution; pH = 1.88; n = 6). The other specimen served as control (A. demin). After water rinsing, elemental composition (Ce, Sm, Ca, P, O, N, Na, Mg, C) was measured (EDX; EDAX Octane-Elect, APEX v2.5, low-vacuum) in dentin. Atomic percent (At%), Ca/P- and Ca/N-ratios were calculated and analyzed non-parametrically (α = 0.05, error rates method). Additionally, antibacterial activity (2 min exposure) of Ce(NO₃)₃ and Sm(NO₃)₃ against Streptococcus mutans, Actinomyces naeslundii, Schaalia odontolytica, and Enterococcus faecalis was determined (colony forming units) after anaerobic incubation at 37 °C for 24 h (control: 0.2 % CHX).

Results

At% (median) of Ce and Sm were as follows: Ce(NO₃)₃ 3.4 and 0.9 At%Ce with and without smear-layer, respectively; Sm(NO₃)₃ 2.4 and 1.3 At%Sm with and without smear-layer, respectively. Ce(NO₃)₃ and Sm(NO₃)₃-application significantly decreased Ca/P-ratios (1.22 – 1.45; p ≤ 0.02) compared to controls (1.47 – 1.63). With smear-layer, significantly higher Ca/N-ratios (5.1 – 29.3) could be detected across all groups (p ≤ 0.004) compared to specimens without smear-layer (0.37 – 0.48). Ce(NO₃)₃ and Sm(NO₃)₃ showed reduction rates of up to ≥ 5 log10 steps for S. mutans, A. naeslundii, and S. odontolytica.

Conclusions

Cerium and samarium nitrate showed accumulation on dentin and certain antibacterial activity and could therefore be identified as potential compounds to treat and prevent dentin and root caries and dentin hypersensitivity.

目的研究硝酸铈和硝酸钐在无涂抹层或有涂抹层情况下在牙本质上的累积情况，并测试它们的抗菌活性。其中 12 片牙本质-釉质切片进行了涂抹层形成（320 grit，200 g，5 秒），另外 12 片牙本质-釉质切片进行了涂抹层去除（20 % EDTA，300 秒）。切片对半切割成 48 个半月形标本。每颗牙齿的一个标本用 Ce(NO3)3（50 wt%水溶液；pH = 1.29；n = 6）或 Sm(NO3)3（50 wt%水溶液；pH = 1.88；n = 6）处理。另一个样本作为对照（A. demin）。水冲洗后，测量牙本质中的元素组成（Ce、Sm、Ca、P、O、N、Na、Mg、C）（EDX；EDAX Octane-Elect，APEX v2.5，低真空）。计算了原子百分比（At%）、Ca/P- 和 Ca/N 比率，并进行了非参数分析（α = 0.05，误差率法）。此外，还测定了 Ce(NO3)3 和 Sm(NO3)3 在 37 ℃厌氧培养 24 小时后（对照组：0.2 % CHX）对变异链球菌、奈氏放线菌、Schaalia odontolytica 和粪肠球菌的抗菌活性（接触 2 分钟）（菌落形成单位）：有涂抹层和无涂抹层时，Ce（NO3）3 的 At%Ce 分别为 3.4 和 0.9；有涂抹层和无涂抹层时，Sm（NO3）3 的 At%Sm 分别为 2.4 和 1.3。与对照组（1.47 - 1.63）相比，施用 Ce(NO3)3 和 Sm(NO3)3 可显著降低 Ca/P 比率（1.22 - 1.45；p ≤ 0.02）。与没有涂抹层的标本（0.37 - 0.48）相比，涂抹了涂抹层的标本在所有组别中都能检测到明显较高的 Ca/N 比值（5.1 - 29.3）（p ≤ 0.004）。结论硝酸铈和硝酸钐在牙本质上有蓄积作用，并具有一定的抗菌活性，因此可被确定为治疗和预防牙本质和牙根龋齿以及牙本质过敏症的潜在化合物。

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引用次数: 0

Differential impact of chronic intermittent hypoxia and stress changes on condylar development 慢性间歇性缺氧和应激变化对髁突发育的不同影响

IF 2.2 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2024-07-15 DOI: 10.1016/j.archoralbio.2024.106051

Objectives

This study aimed to determine the effects of chronic intermittent hypoxia (CIH) and stress change (SC) on the development of the condyle in mouth breathing rats.

Design

A total of 120 4-week-old rats were randomly assigned to one of five groups. The control (Ctrl) group was the blank control and the intermittent nasal obstruction (INO) group was the positive control. Mild CIH (mCIH) and severe CIH (sCIH) groups were developed by adjusting environmental oxygen concentration and monitoring real-time blood oxygen saturation (SpO₂). The SC group was developed using INO, increased environmental oxygen concentration, and real-time SpO₂ monitoring. Six rats from each group were sacrificed for analysis at 0, 1, 2, or 4 weeks.

Results

Similar to the INO group, condyle and mandibular body development in the sCIH group, but not in the mCIH group, was significantly inhibited compared with the Ctrl group. The SC group had inhibited development of the condyle, especially of the posterior zone, but had minimal impact on the growth of the mandible.

Conclusion

The inhibitory effects of CIH on the development of the condyle and mandibular body were SpO₂-dose-dependent. When SC occurred, inhibited development was observed in the posterior zone of condyle but not the whole mandible. These findings provide important insights for targeted interventions that address the consequences of mouth breathing in children.

目的本研究旨在确定慢性间歇性缺氧（CIH）和应激变化（SC）对口呼吸大鼠髁状突发育的影响。对照（Ctrl）组为空白对照组，间歇性鼻阻塞（INO）组为阳性对照组。轻度 CIH（mCIH）组和重度 CIH（sCIH）组是通过调整环境氧浓度和监测实时血氧饱和度（SpO2）而分出的。SC 组采用 INO、增加环境氧浓度和实时 SpO2 监测。结果与 INO 组相似，与 Ctrl 组相比，sCIH 组（而非 mCIH 组）的髁突和下颌骨体发育受到显著抑制。结论 CIH 对髁突和下颌骨体发育的抑制作用与 SpO2 剂量有关。当SC发生时，髁突后部区域的发育受到抑制，而整个下颌骨的发育没有受到抑制。这些发现为有针对性地干预儿童口呼吸后果提供了重要启示。

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引用次数: 0

Morphological characteristics of non-carious cervical lesions. A systematic review 非carious宫颈病变的形态特征。系统回顾

IF 2.2 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2024-07-14 DOI: 10.1016/j.archoralbio.2024.106050

Objectives

This systematic review assessed the morphological characteristics of non-carious cervical lesions (NCCL), among clinical and ex-vivo studies assessed by observational and imaging methods.

Design

The search strategy was conducted on four online databases (MEDLINE, Embase, Scopus and Web of Science) and grey literature (Google Scholar and OpenGrey). The study selection was performed by three reviewers from March to September (2023). The eligibility criteria were established according to the PICO strategy and included NCCL, morphological characteristics and clinical and ex-vivo study designs. The data extraction considered general data that identifies the study, evaluation method, parameter to assess the outcome and the main results for each study. The risk of bias was evaluated using Joanna Briggs Institute critical appraisal tool, and a personalized tool.

Results

The search resulted in 252 studies. A total of 14 studies were included. Prevalence of NCCLs ranged from 3.5 %to 77.78 % with a higher presence in premolars. Common characteristics were wear facets, occluded tubules or cracks, occlusal stress, scratch marks, dimples and craters, structure loss, and dentin sclerosis, which appear more often on buccal surface and were generally classified as wedge-shaped, saucer-shaped. Etiological hypothesis was mainly related to multifactorial factors. In most of the studies, the risk of bias was classified as high.

Conclusions

The morphological characteristics of NCCL showed a wide range of descriptions regarding appearance, prevalence, lesion-related measures, and macro and microscopic descriptions.

目的本系统综述评估了非龋性宫颈病变（NCCL）的形态特征，包括通过观察和成像方法评估的临床和体外研究。设计检索策略在四个在线数据库（MEDLINE、Embase、Scopus 和 Web of Science）和灰色文献（Google Scholar 和 OpenGrey）中进行。研究筛选工作由三位审稿人在 2023 年 3 月至 9 月期间完成。资格标准是根据 PICO 策略制定的，包括 NCCL、形态特征以及临床和体外研究设计。数据提取考虑了一般数据，这些数据确定了每项研究的研究内容、评价方法、结果评估参数和主要结果。使用乔安娜-布里格斯研究所的关键评估工具和个性化工具对偏倚风险进行了评估。共纳入 14 项研究。NCCL的患病率从3.5%到77.78%不等，其中前磨牙的患病率较高。常见的特征有磨损面、咬合小管或裂纹、咬合应力、划痕、凹陷和坑洞、结构缺损和牙本质硬化，这些现象多出现在颊面，一般分为楔形和碟形。病因假设主要与多因素有关。结论NCCL的形态特征在外观、患病率、病变相关测量以及宏观和微观描述方面有广泛的描述。

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引用次数: 0