Archives of oral biology最新文献_第3页

Antibacterial and anti-virulence activity of juglone against Enterococcus faecalis compared with chlorhexidine 核桃酮与氯己定对粪肠球菌的抑菌抑毒作用比较。

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2026-01-08 DOI: 10.1016/j.archoralbio.2026.106511

Rawan Yassin Nimir , Homa Darmani

Objective(s)

We compared the antimicrobial and antivirulence activities of juglone, a plant-derived naphthoquinone, with chlorhexidine (CHX), a widely used dental antiseptic, against Enterococcus faecalis, a pathogen associated with persistent oral and endodontic infections.

Design

Antimicrobial efficacy was evaluated using well diffusion, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) assays. Possible interactions between juglone and CHX were investigated using well diffusion and checkerboard methods. Antivirulence activity was assessed by measuring secreted enzymes (caseinase, gelatinase, and hemolysin), biofilm formation, metabolic activity, extracellular polymeric substance (EPS) production, and disruption of mature biofilms. Oxidative stress responses were examined through lactate dehydrogenase (LDH) release, malondialdehyde (MDA) levels, superoxide dismutase (SOD) activity, and total antioxidant capacity (T-AOC).

Results

Juglone showed antibacterial activity with MICs in a similar range to CHX; however, no synergistic interaction between the two agents was observed. Juglone strongly inhibited caseinase, gelatinase, and hemolysin, whereas CHX showed no effect on caseinase and weaker inhibition of the others. CHX more effectively reduced early biofilm formation, metabolic activity, and mature biomass, whereas both agents similarly decreased EPS production and viability during biofilm development. Notably, in preformed biofilms, juglone at 16 ×MIC caused greater killing (∼4-log₁₀ CFU) than CHX (∼2-log₁₀). Both compounds increased LDH release and reduced SOD activity and T-AOC, while juglone uniquely decreased MDA levels, indicating additional redox-modulating effects.

Conclusions

Juglone impairs E. faecalis virulence by reducing enzymatic activity, oxidative stress tolerance, and biofilm viability, with stronger effects on established biofilms, highlighting its potential for further investigation in oral infection management.

目的：我们比较了木酚酮（一种植物来源的萘醌）和氯己定（一种广泛使用的牙科杀菌剂）对粪肠球菌（一种与口腔和牙髓持续感染相关的病原体）的抗菌和抗病毒活性。设计：采用孔扩散、最小抑菌浓度（MIC）和最小杀菌浓度（MBC）试验评估抗菌效果。采用井扩散法和棋盘法研究了核桃酮与CHX之间可能的相互作用。通过测定分泌酶（酪蛋白酶、明胶酶和溶血素）、生物膜形成、代谢活性、细胞外聚合物（EPS）的产生和成熟生物膜的破坏来评估抗毒活性。通过乳酸脱氢酶（LDH）释放、丙二醛（MDA）水平、超氧化物歧化酶（SOD）活性和总抗氧化能力（T-AOC）检测氧化应激反应。结果：核桃酮对mic的抑菌作用范围与CHX相似；然而，没有观察到两种药物之间的协同作用。核桃酮对酪酶、明胶酶和溶血素有较强的抑制作用，而CHX对酪酶无明显抑制作用，对其他酶的抑制作用较弱。CHX更有效地降低了早期生物膜的形成、代谢活性和成熟生物量，而这两种药物在生物膜发育过程中同样降低了EPS的产生和活力。值得注意的是，在预成型的生物膜中，16 ×MIC的juglone比CHX （~ 2-log10）造成更大的杀伤（~ 4-log10 CFU）。这两种化合物都增加了LDH释放，降低了SOD活性和T-AOC，而核桃酚酮独特地降低了MDA水平，表明了额外的氧化还原调节作用。结论：核桃酮通过降低酶活性、氧化应激耐受性和生物膜活力来损害粪肠杆菌的毒力，对已建立的生物膜有更强的影响，突出了其在口腔感染管理方面的进一步研究潜力。

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引用次数: 0

HOXD10 expression in oral cancer is driven by histone acetylation rather than promoter DNA methylation HOXD10在口腔癌中的表达是由组蛋白乙酰化而不是启动子DNA甲基化驱动的

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2026-01-08 DOI: 10.1016/j.archoralbio.2026.106512

Dhanraj Salur Basavarajappa , U Sangeetha Shenoy , Naveena Kumar AN , Shama Prasada Kabekkodu , Sanjiban Chakrabarty , Raghu Radhakrishnan

Objective

HOXD10 is implicated in the carcinogenesis and progression of various cancers, including oral cancer. However, its regulatory mechanism and functional role remain unclear.

Design

Matched normal and oral cancer tissue samples from patients stratified into oral potentially malignant disorders (OPMD, n = 25), lymph-node negative (LN(-), n = 25), and lymph-node positive (LN(+), n = 25) groups were analysed along with a panel of oral cancer cell lines. Expression was quantified by qRT-PCR and DNA methylation was profiled by methyl-capture sequencing followed by correlation analysis with expression. The DNA methyltransferase inhibitor(DNMTi): Decitabine(5-Aza-CdR) and histone deacetylase inhibitors(HDACi) – suberoylanilide hydroxamic acid(SAHA) and sodium butyrate(NaB) were used to evaluate epigenetic regulation in SCC9 cells. Promoter regions were characterized by dual luciferase assay and sodium butyrate effects on cell proliferation, migration and cell cycle were assessed.

Results

HOXD10 was significantly upregulated in OPMD and LN(+) groups and oral cancer cell lines but not in the LN(-) samples or across cancer stages/grades compared with normal controls. Promoter hypermethylation correlated positively, though not significantly, with expression. 5-Aza-CdR treatment did not alter HOXD10 levels, while HDACi treatment reduced expression. Two promoter regions were identified as active regulatory elements modulated by histone acetylation. Functionally, NaB impaired cell proliferation, migration and induced G2-M arrest. Survival analysis demonstrated modest prognostic value of HOXD10 alone but improved predictive accuracy for disease recurrence when integrated with tumor stage and grade.

Conclusion

HOXD10 expression in oral cancer is regulated predominantly via histone acetylation. These findings highlight its epigenetic regulation and suggest potential clinical relevance with tumor stage and grade although further studies are needed to confirm diagnostic or prognostic value.

目的：hoxd10参与多种癌症的发生和发展，包括口腔癌。但其调控机制和功能作用尚不清楚。设计将匹配的正常和口腔癌组织样本分为口腔潜在恶性疾病（OPMD, n = 25）、淋巴结阴性（LN(-), n = 25）和淋巴结阳性（LN(+), n = 25）组，并对一组口腔癌细胞系进行分析。通过qRT-PCR定量表达，通过甲基捕获测序分析DNA甲基化，然后进行与表达的相关性分析。采用DNA甲基转移酶抑制剂（DNMTi）：地西他滨（5-Aza-CdR）和组蛋白去乙酰化酶抑制剂(HDACi) -亚eroylanilide羟肟酸（SAHA）和丁酸钠（NaB）评估SCC9细胞的表观遗传调控。用双荧光素酶法对启动子区域进行了表征，并评估了丁酸钠对细胞增殖、迁移和细胞周期的影响。结果与正常对照相比，OPMD组、LN（+）组和口腔癌细胞系中shoxd10表达显著上调，而LN（-）组和不同癌症分期/分级中shoxd10表达均不上调。启动子超甲基化与表达呈正相关，但不显著。5-Aza-CdR治疗没有改变HOXD10水平，而HDACi治疗降低了HOXD10的表达。两个启动子区域被确定为由组蛋白乙酰化调节的活性调控元件。功能上，NaB损害细胞增殖、迁移和诱导G2-M阻滞。生存分析显示HOXD10单独的预后价值不大，但当结合肿瘤分期和分级时，疾病复发的预测准确性提高。结论hoxd10在口腔癌组织中的表达主要受组蛋白乙酰化调控。这些发现强调了其表观遗传调控，并提示与肿瘤分期和分级的潜在临床相关性，尽管需要进一步的研究来确认诊断或预后价值。

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引用次数: 0

The exposome and the human oral microbiome through the one health lens 暴露体和人类口腔微生物组通过一个健康镜头。

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2026-01-08 DOI: 10.1016/j.archoralbio.2026.106504

Luis Limo , Justin Donovan , Stephanie Frisbee , Noha Gomaa

Objectives

To map and synthesise current evidence on how lifelong biological, environmental, and social exposures, collectively conceptualised as the exposome, interact with the human oral microbiome to influence oral disease development and progression within a One Health framework.

Design

A scoping review was conducted to identify peer-reviewed studies published in English that examined the relationships between the exposome and the human oral microbiome. The review followed the Arksey and O’Malley framework, applying its five-stage methodological approach. Comprehensive searches were performed in MEDLINE, Embase, PsycInfo, Scopus, Web of Science, and CINAHL. Study quality was assessed using the Joanna Briggs Institute (JBI) tools, and results were reported in accordance with PRISMA-ScR guidelines.

Results

A total of twenty-nine studies were included in this review. These showed that health status, non-communicable diseases, medication use, and psychosocial factors influence the biodiversity, abundance, and function of the human oral microbiome. Other studies suggested that animal interactions and physical and chemical exposures can alter host-microbiome interactions, as well as microbial community dynamics within the oral cavity. While the studies reviewed used reliable methods and standardized protocols, they were of moderate quality due to small sample sizes, potential reverse causality, and limited control for confounding and multiple testing.

Conclusion

This review highlights the complexity of the human oral microbiome and its interactions with the various components of the exposome, emphasizing the focus on disease impact and health behaviours, while noting a gap in research on non-bacterial communities, interaction mechanisms, and long-term effects on dysbiosis.

目标：绘制和综合目前的证据，说明终身生物、环境和社会暴露（统称为暴露者）如何与人类口腔微生物群相互作用，从而影响“同一个健康”框架下口腔疾病的发展和进展。设计：进行了一项范围审查，以确定发表的同行评议的英文研究，这些研究检查了暴露体与人类口腔微生物群之间的关系。该评估遵循Arksey和O'Malley框架，采用其五阶段方法方法。在MEDLINE、Embase、PsycInfo、Scopus、Web of Science和CINAHL中进行综合检索。使用Joanna Briggs Institute （JBI）工具评估研究质量，并根据PRISMA-ScR指南报告结果。结果：本综述共纳入29项研究。这些研究表明，健康状况、非传染性疾病、药物使用和社会心理因素会影响人类口腔微生物群的生物多样性、丰度和功能。其他研究表明，动物相互作用和物理和化学暴露可以改变宿主-微生物组相互作用，以及口腔内微生物群落动态。虽然回顾的研究使用了可靠的方法和标准化的方案，但由于样本量小，潜在的反向因果关系，以及对混杂和多重测试的控制有限，它们的质量一般。结论：本综述强调了人类口腔微生物群的复杂性及其与暴露体各组成部分的相互作用，强调了疾病影响和健康行为的重点，同时指出了非细菌群落、相互作用机制和对生态失调的长期影响的研究空白。

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引用次数: 0

Investigating the mechanism of berberine in inhibiting periodontitis progression using machine learning, molecular docking, and experimental validation 利用机器学习、分子对接和实验验证研究小檗碱抑制牙周炎进展的机制

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2026-01-06 DOI: 10.1016/j.archoralbio.2025.106496

Zhonghua Wang , Zhenli Liu , Sai Ma , Xiaohui Jia , Xu Zhang , Zhiyi Zhang , Chao Li , Jinping Xiao , Yan Si

Background

Periodontitis is a chronic inflammatory disease characterized by the destruction of periodontal tissues. Berberine (BBR), a natural alkaloid with anti-inflammatory and antioxidant properties, has shown potential in treating inflammatory diseases.

Methods

Differentially expressed genes (DEGs) between periodontitis and healthy gingival tissues were identified using the GSE23586 and GSE173078 datasets, while chronic periodontitis-related genes were retrieved from the GeneCards database. Key genes were screened through support vector machine (SVM) and random forest (RF) algorithms, molecular docking, and normal mode analysis. In vitro, human gingival fibroblast-1 (HGF-1) cells were treated with lipopolysaccharide (LPS) to simulate periodontitis.

Results

Six key genes (KRT14, IGFBP6, LRG1, MZB1, DEFB103A, and CD79A) were identified by both SVM and RF algorithms. LRG1 was selected for further study due to its significant downregulation after BBR treatment in LPS-treated HGF-1 cells. LPS treatment increased LRG1 expression and the p-p38/p38 ratio, whereas these effects reversed by BBR treatment. Overexpression of LRG1 diminished BBR’s inhibitory effect on p-p38/p38, while the p38 MAPK pathway inhibitor restored BBR’s efficacy. BBR treatment suppressed LPS-induced inflammatory response, oxidative stress, and ECM degradation, but these effects were relieved by ectopic LRG1 expression.

Conclusion

BBR alleviated LPS-induced periodontitis by inhibiting inflammation, oxidative stress, and ECM degradation through inactivation of the LRG1/p38 MAPK signaling pathway. These findings highlight BBR’s potential as a therapeutic agent for periodontitis, offering a novel molecular target for clinical intervention. Further in vivo studies are warranted to validate its clinical application.

牙周炎是一种以牙周组织破坏为特征的慢性炎症性疾病。小檗碱（BBR）是一种具有抗炎和抗氧化特性的天然生物碱，在治疗炎症性疾病方面已显示出潜力。方法使用GSE23586和GSE173078数据集鉴定牙周炎与健康牙龈组织之间的差异表达基因（DEGs），并从GeneCards数据库检索慢性牙周炎相关基因。通过支持向量机（SVM）和随机森林（RF）算法、分子对接和正态分析筛选关键基因。在体外，用脂多糖（LPS）处理人牙龈成纤维细胞-1 （HGF-1）细胞，模拟牙周炎。结果通过SVM和RF算法分别鉴定出KRT14、IGFBP6、LRG1、MZB1、DEFB103A和CD79A 6个关键基因。LRG1在lps处理的HGF-1细胞中经过BBR处理后显著下调，因此我们选择LRG1作为进一步研究的对象。LPS处理增加了LRG1表达和p-p38/p38比值，而BBR处理逆转了这些作用。过表达LRG1可减弱BBR对p-p38/p38的抑制作用，而p38 MAPK通路抑制剂可恢复BBR的抑制作用。BBR治疗抑制lps诱导的炎症反应、氧化应激和ECM降解，但这些作用通过异位LRG1表达而缓解。结论bbr通过抑制LRG1/p38 MAPK信号通路的失活，抑制炎症、氧化应激和ECM降解，从而减轻lps诱导的牙周炎。这些发现突出了BBR作为牙周炎治疗剂的潜力，为临床干预提供了新的分子靶点。需要进一步的体内研究来验证其临床应用。

{"title":"Investigating the mechanism of berberine in inhibiting periodontitis progression using machine learning, molecular docking, and experimental validation","authors":"Zhonghua Wang , Zhenli Liu , Sai Ma , Xiaohui Jia , Xu Zhang , Zhiyi Zhang , Chao Li , Jinping Xiao , Yan Si","doi":"10.1016/j.archoralbio.2025.106496","DOIUrl":"10.1016/j.archoralbio.2025.106496","url":null,"abstract":"<div><h3>Background</h3><div>Periodontitis is a chronic inflammatory disease characterized by the destruction of periodontal tissues. Berberine (BBR), a natural alkaloid with anti-inflammatory and antioxidant properties, has shown potential in treating inflammatory diseases.</div></div><div><h3>Methods</h3><div>Differentially expressed genes (DEGs) between periodontitis and healthy gingival tissues were identified using the GSE23586 and GSE173078 datasets, while chronic periodontitis-related genes were retrieved from the GeneCards database. Key genes were screened through support vector machine (SVM) and random forest (RF) algorithms, molecular docking, and normal mode analysis. In vitro, human gingival fibroblast-1 (HGF-1) cells were treated with lipopolysaccharide (LPS) to simulate periodontitis.</div></div><div><h3>Results</h3><div>Six key genes (KRT14, IGFBP6, LRG1, MZB1, DEFB103A, and CD79A) were identified by both SVM and RF algorithms. LRG1 was selected for further study due to its significant downregulation after BBR treatment in LPS-treated HGF-1 cells. LPS treatment increased LRG1 expression and the p-p38/p38 ratio, whereas these effects reversed by BBR treatment. Overexpression of LRG1 diminished BBR’s inhibitory effect on p-p38/p38, while the p38 MAPK pathway inhibitor restored BBR’s efficacy. BBR treatment suppressed LPS-induced inflammatory response, oxidative stress, and ECM degradation, but these effects were relieved by ectopic LRG1 expression.</div></div><div><h3>Conclusion</h3><div>BBR alleviated LPS-induced periodontitis by inhibiting inflammation, oxidative stress, and ECM degradation through inactivation of the LRG1/p38 MAPK signaling pathway. These findings highlight BBR’s potential as a therapeutic agent for periodontitis, offering a novel molecular target for clinical intervention. Further in vivo studies are warranted to validate its clinical application.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"183 ","pages":"Article 106496"},"PeriodicalIF":2.1,"publicationDate":"2026-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145973709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antimicrobial effect of garlic-based mouthwash on dental caries-related oral microorganisms: A systematic review and meta-analysis 大蒜漱口水对龋齿相关口腔微生物的抗菌作用：系统综述和荟萃分析。

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2025-12-31 DOI: 10.1016/j.archoralbio.2025.106495

Renata de Oliveira Alves , José Roberto Vergínio de Matos , Matheus Henrique Faccioli Ragghianti , Isabela Maria Passarela Gomes , Isabela dos Santos de Deus , Pamela Barbosa dos Santos , Marcelle Danelon , Gabriel Pereira Nunes

Objective

This systematic review and meta-analysis (SRM) investigated the potential of garlic (Allium sativum) against cariogenic oral microorganisms.

Design

The SRM was conducted following PRISMA guidelines and registered in PROSPERO (CRD420251133140). Randomized clinical trials (RCTs) evaluating garlic or garlic-derived compounds on cariogenic oral microorganisms were included. A literature search was performed in the main scientific databases. Meta-analyses were performed using RevMan software, with standardized mean difference (SMD) as the effect measure, and a random-effects model was applied with 95 % confidence intervals. Risk of bias was assessed using the Cochrane tool, and the certainty of evidence was graded according to the GRADE approach.

Results

Nine RCTs fulfilled the inclusion criteria, predominantly assessing S. mutans, followed by Lactobacillus spp. and C. albicans. All included trials employed garlic-based mouthwashes as the intervention and consistently demonstrated significant antimicrobial activity. In meta-analysis, compared to chlorhexidine, garlic reduced S. mutans at 1 week (SMD = −0.73, 95 % CI = −1.39 to −0.07, I² = 73 %; p = 0.03), had a slightly lower effect at 2 weeks (SMD = 1.27, 95 % CI = 0.09–2.44, I² = 90 %; p = 0.03), and showed no difference at 1 month (SMD = −0.54, 95 % CI = −2.78–1.70, I² = 96 %; p = 0.64). Compared to sodium fluoride, it demonstrated superior activity at 2 weeks (SMD = −0.79, 95 % CI = −1.22 to −0.36, I² = 0 %; p = 0.0003). Most studies had a low risk of bias, and the certainty of the evidence was rated low.

Conclusions

Garlic-based mouthrinses show significant antimicrobial activity against cariogenic microorganisms, supporting their potential as a phytotherapeutic strategy for biofilm control. However, the evidence remains limited, demonstrating the need for further high-quality clinical trials to confirm long-term efficacy.

目的：本系统综述和荟萃分析（SRM）探讨大蒜（Allium sativum）对口腔致龋微生物的潜在作用。设计：SRM按照PRISMA指南进行，并在PROSPERO注册（CRD420251133140）。随机临床试验（rct）评估大蒜或大蒜衍生化合物对龋病口腔微生物。在主要科学数据库中进行文献检索。采用RevMan软件进行meta分析，采用标准化平均差（SMD）作为效应测度，采用随机效应模型，置信区间为95% %。使用Cochrane工具评估偏倚风险，并根据GRADE方法对证据的确定性进行分级。结果：9项随机对照试验符合纳入标准，主要评估变形链球菌，其次是乳酸杆菌和白色念珠菌。所有纳入的试验均采用大蒜漱口水作为干预措施，并始终显示出显著的抗菌活性。在荟萃分析,洗必泰相比,大蒜降低变形链球菌在1周(SMD = -0.73, 95 % CI = -1.39 - -0.07,我²= 73 %;p = 0.03),略低的影响在两周(SMD = 1.27, 95 % CI = 0.09 - -2.44,我²= 90 %;p = 0.03),并显示在1个月没有区别(SMD = -0.54, 95 % CI = -2.78 - -1.70,我²= 96 %;p = 0.64)。与氟化钠相比，它在2周时表现出更好的活性（SMD = -0.79, 95 % CI = -1.22至-0.36,I²= 0 %;p = 0.0003）。大多数研究的偏倚风险较低，证据的确定性评级较低。结论：大蒜漱口水对致龋微生物具有显著的抗菌活性，支持其作为生物膜控制的植物治疗策略的潜力。然而，证据仍然有限，表明需要进一步的高质量临床试验来确认长期疗效。

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引用次数: 0

Corrigendum to “Association between agenesis of permanent teeth and other dental anomalies in nonsyndromic patients: Systematic review” [Archives of Oral Biology 173 (2025) 106223] “非综合征患者恒牙发育与其他牙齿畸形的关系：系统综述”[口腔生物学档案173(2025)106223]。

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2025-12-30 DOI: 10.1016/j.archoralbio.2025.106490

Paula Cristiana Santos de Aguiar , Caio Melo Mesquita , Fabiana Evangelista Lerner , Laura Bezerra Borges , Rodrigo Rodrigues , Walbert A. Vieira , Luiz Renato Paranhos , Rui Barbosa de Brito Júnior

引用次数: 0

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2025-12-30 DOI: 10.1016/j.archoralbio.2025.106494

Xueyi Liang, Runxi Fu, Xiaochuan Chen

Background

Exosomes and lactylation modification have been increasingly recognized as key regulators of diseases, yet their integrative role in periodontitis remains unclear. No diagnostic model based on exosome-related lactylation genes (ERLGs) has been previously established for periodontitis. This study aimed to explore ERLGs as potential diagnostic biomarkers for periodontitis.

Methods

Integration of bulk and single-cell RNA sequencing (scRNA-seq) datasets, machine learning modeling, and experimental validation were employed to identify ERLG signatures and dissect their cellular context in periodontitis.

Result

We identified 53 ERLGs that were significantly dysregulated in periodontitis and closely linked to metabolic reprogramming and immune regulation. ERLG-based clustering robustly distinguished periodontitis from healthy tissues and revealed distinct immune infiltration patterns. A machine learning–based diagnostic model using eight core ERLGs (AK3, CHST1, CHST2, MERTK, EGLN3, CXCR4, DSC2, KCNN4) achieved excellent predictive performance (AUC=0.938 in training cohort; validated in two independent cohorts). scRNA-seq uncovered heterogeneous lactylation modification patterns across major cell populations. Fibroblast subclustering revealed three disease-sensitive subsets (Fib_NTRK3, Fib_TNXB, Fib_MFAP5) that were reduced in periodontitis and exhibited reduced lactylation scores. Endothelial cell subclustering identified a disease-enriched Endo_TGM2 population characterized by high lactylation scores, activation of TGF-β, PI3K–AKT–mTOR, and TNFα/NF-κB signaling, and dynamic differentiation trajectories, and strong interactions with fibroblasts. Inflammatory stimulation enhanced exosomal lactylation and derived ERLG dysregulation in human gingival fibroblasts.

Conclusion

This study establishes the first ERLG-based diagnostic model for periodontitis, demonstrates its robust predictive capability and delineates cell type–specific lactylation remodeling, which providing mechanistic insights and potential signature for diagnosis.

背景：外泌体和乳酸化修饰越来越被认为是疾病的关键调节因子，但它们在牙周炎中的综合作用尚不清楚。目前尚无基于外泌体相关乳酸化基因（ERLGs）的牙周炎诊断模型。本研究旨在探索ERLGs作为牙周炎潜在的诊断生物标志物。方法：整合大量和单细胞RNA测序（scRNA-seq）数据集，机器学习建模和实验验证，以识别ERLG特征并解剖其在牙周炎中的细胞背景。结果：我们确定了53个ERLGs在牙周炎中显着失调，并与代谢重编程和免疫调节密切相关。基于erlg的聚类可以将牙周炎与健康组织区分开来，并显示出不同的免疫浸润模式。使用8个核心ERLGs （AK3、CHST1、CHST2、MERTK、EGLN3、CXCR4、DSC2、KCNN4）的基于机器学习的诊断模型获得了出色的预测性能（AUC=0.938，在两个独立的队列中得到验证）。scRNA-seq揭示了主要细胞群中异构的乳酸化修饰模式。成纤维细胞亚聚类揭示了三个疾病敏感亚群（Fib_NTRK3, Fib_TNXB, Fib_MFAP5）在牙周炎中减少，并表现出降低的酰化评分。内皮细胞亚聚类鉴定出一个疾病富集的Endo_TGM2群体，其特征是高乳酸化评分、TGF-β、PI3K-AKT-mTOR和TNFα/NF-κB信号的激活、动态分化轨迹以及与成纤维细胞的强相互作用。炎症刺激增强了人牙龈成纤维细胞的外泌体乳酸化和衍生的ERLG失调。结论：本研究建立了首个基于ergl的牙周炎诊断模型，显示了其强大的预测能力，并描绘了细胞类型特异性的乳酸化重塑，为诊断提供了机制见解和潜在的特征。

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引用次数: 0

Changes in the craniofacial morphology in sclerostin knockout mice 硬化蛋白敲除小鼠颅面形态的变化

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2025-12-29 DOI: 10.1016/j.archoralbio.2025.106492

Kazutaka Ikeda , Masato Kaku , Chao Fan-Yi , Chisa Shukunami , Kotaro Tanimoto

Objectives

To investigate the influence of lack of sclerostin on craniofacial morphology in sclerostin gene (Sost) knockout (Sost^Δ26/Δ26) mice.

Design

Micro computed tomography (micro-CT) was performed on both 60-day-old wild-type and Sost^Δ26/Δ26 mice to analyze changes in craniofacial morphology. Ten anatomical landmarks and eight measurement parameters as well as the total mandibular volume were used to assess craniofacial features. In addition, the femora of both wild-type and Sost^Δ26/Δ26 mice were examined using micro-CT and histological analysis. Cancellous bone volume, cortical bone volume, and the number of osteoclasts beneath the growth plate in the femora were evaluated.

Results

The neurocranium was significantly smaller in the Sost^Δ26/Δ26 mice than in the wild-type mice, while the length of the angular process of the mandible was significantly greater. The total mandibular volume was significantly larger in the Sost^Δ26/Δ26 mice than in the wild-type mice. The volume of cancellous bone and cortical bone in the femora were significantly larger in the Sost^Δ26/Δ26 mice than in the littermate wild-type mice. Histological observation showed no statistically significant difference in the number of osteoclasts present in the femora between wild-type mice and Sost^Δ26/Δ26 mice.

Conclusions

The present study showed a significant decrease in neurocranium and a significant increase in mandibular bone in Sost^Δ26/Δ26 mice compared to wild-type mice, along with increased volumes of cancellous and cortical bone in the femora.

目的探讨硬化蛋白基因（Sost）敲除（SostΔ26/Δ26）小鼠颅面形态变化对硬化蛋白缺失的影响。对60日龄野生型小鼠和SostΔ26/Δ26小鼠进行显微计算机断层扫描（micro-CT），分析颅面形态的变化。采用10个解剖标志和8个测量参数以及下颌总容积来评估颅面特征。此外，对野生型和SostΔ26/Δ26小鼠的股骨进行显微ct检查和组织学分析。评估股骨生长板下松质骨体积、皮质骨体积和破骨细胞数量。结果SostΔ26/Δ26小鼠神经头盖骨明显小于野生型小鼠，下颌骨角突长度明显大于野生型小鼠。SostΔ26/Δ26小鼠的下颌总体积明显大于野生型小鼠。SostΔ26/Δ26小鼠股骨松质骨和皮质骨体积明显大于同窝野生型小鼠。组织学观察显示，野生型小鼠与SostΔ26/Δ26小鼠股骨破骨细胞数量无统计学差异。本研究显示，与野生型小鼠相比，SostΔ26/Δ26小鼠的神经头盖骨显著减少，下颌骨显著增加，股骨的松质骨和皮质骨体积增加。

{"title":"Changes in the craniofacial morphology in sclerostin knockout mice","authors":"Kazutaka Ikeda , Masato Kaku , Chao Fan-Yi , Chisa Shukunami , Kotaro Tanimoto","doi":"10.1016/j.archoralbio.2025.106492","DOIUrl":"10.1016/j.archoralbio.2025.106492","url":null,"abstract":"<div><h3>Objectives</h3><div>To investigate the influence of lack of sclerostin on craniofacial morphology in sclerostin gene (<em>Sost</em>) knockout (<em>Sost</em><sup><em>Δ26/Δ26</em></sup>) mice.</div></div><div><h3>Design</h3><div>Micro computed tomography (micro-CT) was performed on both 60-day-old wild-type and <em>Sost</em><sup><em>Δ26/Δ26</em></sup> mice to analyze changes in craniofacial morphology. Ten anatomical landmarks and eight measurement parameters as well as the total mandibular volume were used to assess craniofacial features. In addition, the femora of both wild-type and <em>Sost</em><sup><em>Δ26/Δ26</em></sup> mice were examined using micro-CT and histological analysis. Cancellous bone volume, cortical bone volume, and the number of osteoclasts beneath the growth plate in the femora were evaluated.</div></div><div><h3>Results</h3><div>The neurocranium was significantly smaller in the <em>Sost</em><sup><em>Δ26/Δ26</em></sup> mice than in the wild-type mice, while the length of the angular process of the mandible was significantly greater. The total mandibular volume was significantly larger in the <em>Sost</em><sup><em>Δ26/Δ26</em></sup> mice than in the wild-type mice. The volume of cancellous bone and cortical bone in the femora were significantly larger in the <em>Sost</em><sup><em>Δ26/Δ26</em></sup> mice than in the littermate wild-type mice. Histological observation showed no statistically significant difference in the number of osteoclasts present in the femora between wild-type mice and <em>Sost</em><sup><em>Δ26/Δ26</em></sup> mice.</div></div><div><h3>Conclusions</h3><div>The present study showed a significant decrease in neurocranium and a significant increase in mandibular bone in <em>Sost</em><sup><em>Δ26/Δ26</em></sup> mice compared to wild-type mice, along with increased volumes of cancellous and cortical bone in the femora.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"183 ","pages":"Article 106492"},"PeriodicalIF":2.1,"publicationDate":"2025-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145881375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Topical sevoflurane enhances periodontal wound healing in a male rat palatal mucosal model 局部七氟醚促进雄性大鼠腭粘膜模型牙周伤口愈合。

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2025-12-25 DOI: 10.1016/j.archoralbio.2025.106491

Gülenay Çolak , Zeynep Turgut Çankaya , Çiğdem Elmas , Gökçe Nur Arık Erol

Objective

The aim of this study is to evaluate the effect of topical sevoflurane, which has recently been reported to possess analgesic, anti-inflammatory and antibacterial properties, on secondary wound healing in rat palatal mucosa.

Design

Thirty male Wistar Albino rats were randomly divided into three groups: Control, Saline (0.9 % NaCl) and Sevoflurane. Standardized full-thickness excisional wounds (4 mm) were created on the hard palate, and treatment was applied once daily for 14 days. Wound closure, histopathological healing and immunohistochemical expression of vascular endothelial growth factor, interleukin-17A, interleukin-22 and signal transducer and activator of transcription 3 were evaluated on days 5 and 14.

Results

On day 5, the sevoflurane group exhibited smaller wound areas (p < 0.05) and greater angiogenesis, epithelialization, and granulation than the control group. On day 14, epithelial integrity and vascular remodeling were most pronounced in the sevoflurane group. IL-22 and pSTAT3 expression levels were higher, while IL-17A expression was lower (p < 0.05). VEGF expression decreased over time, indicating progression toward tissue remodeling.

Conclusions

Topical sevoflurane enhances early-stage wound healing by modulating inflammatory and regenerative pathways, promoting angiogenesis, and reducing wound size. These findings suggest that sevoflurane may serve as a novel adjunct for periodontal wound healing.

目的：本研究的目的是评价外用七氟醚对大鼠腭黏膜继发性伤口愈合的影响，七氟醚最近被报道具有镇痛、抗炎和抗菌的特性。设计：30只雄性Wistar Albino大鼠随机分为3组：对照组、生理盐水（0.9 % NaCl）组和七氟醚组。在硬腭建立标准化全层切除创面（4 mm），每日1次，连用14天。第5、14天观察创面闭合、组织病理愈合及血管内皮生长因子、白细胞介素- 17a、白细胞介素-22、信号转导因子和转录激活因子3的免疫组化表达。结果：第5天，七氟醚组创面面积较小（p ）结论：局部七氟醚通过调节炎症和再生途径、促进血管生成和减少创面大小来促进早期创面愈合。这些发现表明，七氟醚可能作为一种新的辅助牙周伤口愈合。

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引用次数: 0

Comparative pharmacokinetics of fluoride secretion by major and labial minor salivary glands after oral fluoride intake 口服氟化物后大、唇小唾液腺分泌氟化物的比较药代动力学。

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology

Pub Date : 2025-12-24 DOI: 10.1016/j.archoralbio.2025.106488

Ligiane Campos Bellose, Leonardo Libardi Pagotto, Deborah Rackel Caldas-da-Rocha, Cinthia Pereira Machado Tabchoury, Antônio Pedro Ricomini-Filho, Jaime Aparecido Cury

Objectives

To evaluate whether fluoride secretion by labial minor salivary glands exhibits a pharmacokinetic pattern comparable to that of major salivary glands after oral fluoride intake.

Design

In a randomized, blind and crossover study, eight healthy adult participants ingested aqueous NaF solutions containing 5, 10, or 20 mg of fluoride after overnight fasting. Saliva samples were collected at baseline (time 0) and at 5, 15, 30, 60, and 120 min post-ingestion from four sources: labial minor glands (LG), parotid glands (PG), floor of the mouth (FS), and whole saliva (WS). LG saliva and FS were collected using pipette tips under gentle suction, while PG saliva was obtained using the Carlson-Crittenden device and WS was obtained by asking participants to expectorate directly into a 1.5 mL microtube. Fluoride concentrations were determined using a validated micro-method with an inverted fluoride ion-selective electrode. Pharmacokinetic parameters - maximum concentration (Cmax) and area under the curve (AUC) - were calculated. Data were analyzed by two-way ANOVA and linear regression (α=0.05).

Results

Fluoride secretion from LG demonstrated a dose-dependent response, with Cmax values of 0.37 ± 0.04, 0.45 ± 0.04, and 0.76 ± 0.19 µg/mL, and AUC values of 29.0 ± 6.2, 33.6 ± 3.4, and 52.7 ± 11.5 µg F/mL ×  min for 5, 10, and 20 mg doses, respectively. These parameters did not differ significantly from those obtained from other salivary glands. A significant linear correlation was observed between the ingested fluoride dose and LG fluoride secretion (R² = 0.6469, p < 0.0001).

Conclusion

The pharmacokinetic profile of fluoride secretion in labial minor salivary glands is quantitatively comparable to that of major salivary glands. Their use as an alternative source in fluoride pharmacokinetic studies appears valid, offering a non-invasive and practical sampling method.

目的：评价口服氟化物后，唇侧小唾液腺分泌氟化物的药代动力学模式是否与大唾液腺相似。设计：在一项随机、盲和交叉研究中，8名健康成人受试者在禁食一夜后摄入含有5、10或20 毫克氟化物的NaF水溶液。在基线（时间0）和摄入后5、15、30、60和120 min从四个来源收集唾液样本：唇小腺（LG）、腮腺（PG）、口腔底（FS）和全唾液（WS）。LG唾液和FS唾液采用移液管尖轻轻吸取，PG唾液采用Carlson-Crittenden装置采集，WS唾液采用要求参与者直接呼痰至1.5 mL微管中采集。氟化物浓度的测定采用一种验证的微法与倒置氟离子选择电极。计算最大浓度（Cmax）和曲线下面积（AUC）等药动学参数。数据采用双因素方差分析和线性回归分析（α=0.05）。结果:氟LG响应存在剂量依赖的相关性,分泌物Cmax值为0.37 ± 0.04,0.45 ± 0.04,和0.76 ±0.19  µg / mL,和AUC值29.0 ± 6.2,33.6 ± 3.4,和52.7 ±11.5  µg F /毫升× 为5分钟,10和20 毫克剂量。这些参数与从其他唾液腺获得的参数没有显著差异。氟摄入剂量与LG氟分泌呈显著的线性相关（R²= 0.6469,p ）。结论：唇小唾液腺氟分泌的药动学特征与大唾液腺的药动学特征在数量上具有可比性。它们作为氟化物药代动力学研究的替代来源似乎是有效的，提供了一种非侵入性和实用的采样方法。

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引用次数: 0