This systematic review described Hypoxia-Inducible Factor-1a stabilization or upregulation approaches along with underlying signaling pathways and assessed bone regeneration, angiogenesis, and consolidation time during DO in animal models.
Design
A comprehensive and systematic search of electronic databases including PubMed, Scopus, and ScienceDirect was performed till December 26, 2023. The search was limited to English articles, and no time restrictions were applied.
Results
A total of 14 studies met the inclusion criteria and were included for final review. Four methods have been shown to activate the HIF pathway including genetic, pharmacological, mechanical, and cell preconditioning approaches. Deferoxamine (DFO) was administered as a pharmacological hypoxia-mimicking agent in many studies reporting acceptable outcomes on bone regeneration and acceleration of bone consolation. Applying mechanical loads at the optimal rate and amplitude serves as a minimally invasive approach with acceptable results. HIF-related signaling pathways increase osteogenesis and angiogenesis during DO, potentially through VHL/HIF-1a/VEGF, Wnt/β-catenin, and Mesenchymal-Epithelial transition (MET) signaling pathways.
Conclusion
Activation of HIF-related signaling pathways enhances and accelerates bone regeneration during the consolidation phase of distraction osteogenesis. The most feasible approach with the least side effects must be selected for further clinical studies.
{"title":"The effect of Hypoxia-Inducible Factor-1a stabilization on bone regeneration during distraction osteogenesis: A systematic review of animal studies","authors":"Shaghayegh Najary , Hanieh Nokhbatolfoghahaei , Arash Khojasteh","doi":"10.1016/j.archoralbio.2025.106184","DOIUrl":"10.1016/j.archoralbio.2025.106184","url":null,"abstract":"<div><h3>Objective</h3><div>This systematic review described Hypoxia-Inducible Factor-1a stabilization or upregulation approaches along with underlying signaling pathways and assessed bone regeneration, angiogenesis, and consolidation time during DO in animal models.</div></div><div><h3>Design</h3><div>A comprehensive and systematic search of electronic databases including PubMed, Scopus, and ScienceDirect was performed till December 26, 2023. The search was limited to English articles, and no time restrictions were applied.</div></div><div><h3>Results</h3><div>A total of 14 studies met the inclusion criteria and were included for final review. Four methods have been shown to activate the HIF pathway including genetic, pharmacological, mechanical, and cell preconditioning approaches. Deferoxamine (DFO) was administered as a pharmacological hypoxia-mimicking agent in many studies reporting acceptable outcomes on bone regeneration and acceleration of bone consolation. Applying mechanical loads at the optimal rate and amplitude serves as a minimally invasive approach with acceptable results. HIF-related signaling pathways increase osteogenesis and angiogenesis during DO, potentially through VHL/HIF-1a/VEGF, Wnt/β-catenin, and Mesenchymal-Epithelial transition (MET) signaling pathways.</div></div><div><h3>Conclusion</h3><div>Activation of HIF-related signaling pathways enhances and accelerates bone regeneration during the consolidation phase of distraction osteogenesis. The most feasible approach with the least side effects must be selected for further clinical studies.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"172 ","pages":"Article 106184"},"PeriodicalIF":2.2,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143082617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-22DOI: 10.1016/j.archoralbio.2025.106182
Nehad M. Abd-elmonsif , Sherif Gamal , Sarah Adolf Barsoom
Objectives
Reporting the histological effects of chronic stress on certain oral tissues, as well as the capacity of Thymus vulgaris (thyme) to protect tissues from stress and link both serum cortisol and serotonin levels.
Methods
30 rats were randomly divided into a trio of groups: normal control (no treatment), stress group (chronic stress without treatment), and treatment group (chronic stress treated with thyme at a dose of 200 mg/kg BW orally via needle gavage daily for 21 days). At the end of the experiment, tongues and major sublingual glands (SLGs) were surgically removed and processed for histological and histochemical studies. Blood samples were taken shortly before scarification for the biochemical study of cortisol and serotonin serum levels.
Results
Examination of tongue and SLG sections of the stress group indicated significant alterations in histology and changes in SLG secretion. An examination of tongue and SLG histological sections of the thyme-treated group are showed an improvement. Chronic stress raises cortisol serum levels and lowers serotonin serum levels.
Conclusions
Chronic stress causes alteration of the tongue and major SLG histology, as well as changes in SLG secretion. Thyme may protect tissues from stress, and there is a relation between cortisol and serotonin levels.
{"title":"Chronic stress and depression impact on tongue and major sublingual gland histology and the potential protective role of Thymus vulgaris: An animal study","authors":"Nehad M. Abd-elmonsif , Sherif Gamal , Sarah Adolf Barsoom","doi":"10.1016/j.archoralbio.2025.106182","DOIUrl":"10.1016/j.archoralbio.2025.106182","url":null,"abstract":"<div><h3>Objectives</h3><div>Reporting the histological effects of chronic stress on certain oral tissues, as well as the capacity of Thymus vulgaris (thyme) to protect tissues from stress and link both serum cortisol and serotonin levels.</div></div><div><h3>Methods</h3><div>30 rats were randomly divided into a trio of groups: normal control (no treatment), stress group (chronic stress without treatment), and treatment group (chronic stress treated with thyme at a dose of 200 mg/kg BW orally via needle gavage daily for 21 days). At the end of the experiment, tongues and major sublingual glands (SLGs) were surgically removed and processed for histological and histochemical studies. Blood samples were taken shortly before scarification for the biochemical study of cortisol and serotonin serum levels.</div></div><div><h3>Results</h3><div>Examination of tongue and SLG sections of the stress group indicated significant alterations in histology and changes in SLG secretion. An examination of tongue and SLG histological sections of the thyme-treated group are showed an improvement. Chronic stress raises cortisol serum levels and lowers serotonin serum levels.</div></div><div><h3>Conclusions</h3><div>Chronic stress causes alteration of the tongue and major SLG histology, as well as changes in SLG secretion. Thyme may protect tissues from stress, and there is a relation between cortisol and serotonin levels.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"172 ","pages":"Article 106182"},"PeriodicalIF":2.2,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143049110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The study assessed the prevalence and clinical implications of Epstein Barr Virus (EBV)-positive but Human Papillomavirus (HPV)-negative oral squamous cell carcinoma (OSCC) in a tertiary care hospital setting. The overall goal was to elucidate the potential impact of EBV on OSCC disease progression and prognosis.
Design
A total of 134 surgically resected and histopathologically confirmed OSCC tumor biopsies were collected from a tertiary care hospital. Tumor samples were analyzed for HPV and EBV using conventional and nested PCR. Clinical and demographic data, including age, sex, risk factors, tumor stage, and survival outcomes, were collected and analyzed to assess associations between EBV status and disease characteristics.
Results
All the tumor samples tested negative for HPV. However, EBV was detected in 74 cases (55.3 %) using nested PCR which was confirmed by sanger sequencing. EBV prevalence was higher in males (62.1 %), and the left buccal mucosa was the most affected site, accounting for 34 % of cases. Although statistically not significant, 63.5 % (n = 47) of the EBV positive subjects had a history of consuming both tobacco and alcohol. Of these 20 subjects showing recurrence, 35 % (n = 7) were EBV positive suggesting poor prognosis for EBV positive OSCC subjects.
Conclusion
This study highlights a significant prevalence of EBV in HPV-negative OSCC cases, suggesting a potential oncogenic role for EBV in OSCC progression, particularly in patients with established lifestyle risk factors. These findings underscore the need for further research into EBV's molecular mechanisms in OSCC and its utility as a biomarker for prognosis and therapeutic targeting. The results advocate for region-specific strategies to better understand and manage EBV-associated OSCC, offering potential pathways to improve outcomes in high-risk populations.
{"title":"Association of Epstein-Barr Virus and its clinical relevance in Human Papillomavirus-negative oral squamous cell carcinoma: A cohort study from South India","authors":"Rajesha Payaradka , Pushkal Sinduvadi Ramesh , Vinay Kumar Rajendra , Hosapatna Laxminarayana Kishan Prasad , Prakash Patil , Mohana Kumar , Devanand Devegowda , Veena Shetty","doi":"10.1016/j.archoralbio.2025.106183","DOIUrl":"10.1016/j.archoralbio.2025.106183","url":null,"abstract":"<div><h3>Objective</h3><div>The study assessed the prevalence and clinical implications of Epstein Barr Virus (EBV)-positive but Human Papillomavirus (HPV)-negative oral squamous cell carcinoma (OSCC) in a tertiary care hospital setting. The overall goal was to elucidate the potential impact of EBV on OSCC disease progression and prognosis.</div></div><div><h3>Design</h3><div>A total of 134 surgically resected and histopathologically confirmed OSCC tumor biopsies were collected from a tertiary care hospital. Tumor samples were analyzed for HPV and EBV using conventional and nested PCR. Clinical and demographic data, including age, sex, risk factors, tumor stage, and survival outcomes, were collected and analyzed to assess associations between EBV status and disease characteristics.</div></div><div><h3>Results</h3><div>All the tumor samples tested negative for HPV. However, EBV was detected in 74 cases (55.3 %) using nested PCR which was confirmed by sanger sequencing. EBV prevalence was higher in males (62.1 %), and the left buccal mucosa was the most affected site, accounting for 34 % of cases. Although statistically not significant, 63.5 % (n = 47) of the EBV positive subjects had a history of consuming both tobacco and alcohol. Of these 20 subjects showing recurrence, 35 % (n = 7) were EBV positive suggesting poor prognosis for EBV positive OSCC subjects.</div></div><div><h3>Conclusion</h3><div>This study highlights a significant prevalence of EBV in HPV-negative OSCC cases, suggesting a potential oncogenic role for EBV in OSCC progression, particularly in patients with established lifestyle risk factors. These findings underscore the need for further research into EBV's molecular mechanisms in OSCC and its utility as a biomarker for prognosis and therapeutic targeting. The results advocate for region-specific strategies to better understand and manage EBV-associated OSCC, offering potential pathways to improve outcomes in high-risk populations.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"172 ","pages":"Article 106183"},"PeriodicalIF":2.2,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143049104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study aimed to investigate the effects of nasal obstruction on the coordinated movement of perioral tissues during mastication using a motion capture system.
Design
Twelve healthy adult participants were instructed to chew gum only on their habitual masticatory side for 30 s, with and without nasal obstruction. Nasal obstruction was induced by blocking nasal breathing with a nose clip. Movements of the upper and lower lips, left and right corners of the mouth, and chin during gum chewing were measured using a motion capture system, and the differences between participants with and without nasal obstruction were compared. Multilevel linear model analysis was used to evaluate inter-individual and intra-individual variations in lip and chin movements, both with and without nasal obstruction.
Results
The chin chewing cycle duration was significantly longer in participants with nasal obstruction. The chewing cycle distance of the upper lip was significantly shorter in participants with nasal obstruction. The intra-individual variation in the chewing cycle distance of the chin and lips increased with nasal obstruction, whereas the inter-individual variation decreased.
Conclusions
Nasal obstruction causes unstable yet uniform movements of the chin and lips during mastication, while also restricting upper-lip movement. Our results indicate that nasal obstruction may significantly affect mandibular and lip movements during mastication. These findings highlight the importance of nasal breathing in maintaining stable perioral movements during mastication. This study provides new insights that could inform clinical strategies for managing oral function in individuals with nasal obstruction.
{"title":"Three-dimensional analysis of lip and chin movements during mastication with artificial nasal obstruction","authors":"Yuichi Ariyasu, Yusuke Ban, Emi Inada, Daisuke Murakami, Haruyoshi Yamaza","doi":"10.1016/j.archoralbio.2025.106181","DOIUrl":"10.1016/j.archoralbio.2025.106181","url":null,"abstract":"<div><h3>Objective</h3><div>This study aimed to investigate the effects of nasal obstruction on the coordinated movement of perioral tissues during mastication using a motion capture system.</div></div><div><h3>Design</h3><div>Twelve healthy adult participants were instructed to chew gum only on their habitual masticatory side for 30 s, with and without nasal obstruction. Nasal obstruction was induced by blocking nasal breathing with a nose clip. Movements of the upper and lower lips, left and right corners of the mouth, and chin during gum chewing were measured using a motion capture system, and the differences between participants with and without nasal obstruction were compared. Multilevel linear model analysis was used to evaluate inter-individual and intra-individual variations in lip and chin movements, both with and without nasal obstruction.</div></div><div><h3>Results</h3><div>The chin chewing cycle duration was significantly longer in participants with nasal obstruction. The chewing cycle distance of the upper lip was significantly shorter in participants with nasal obstruction. The intra-individual variation in the chewing cycle distance of the chin and lips increased with nasal obstruction, whereas the inter-individual variation decreased.</div></div><div><h3>Conclusions</h3><div>Nasal obstruction causes unstable yet uniform movements of the chin and lips during mastication, while also restricting upper-lip movement. Our results indicate that nasal obstruction may significantly affect mandibular and lip movements during mastication. These findings highlight the importance of nasal breathing in maintaining stable perioral movements during mastication. This study provides new insights that could inform clinical strategies for managing oral function in individuals with nasal obstruction.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"172 ","pages":"Article 106181"},"PeriodicalIF":2.2,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143049039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-21DOI: 10.1016/j.archoralbio.2025.106179
Andrew Ta , Sonia Nath , Brianna Poirier , Sneha Sethi , Gabrielle Smart , Emilija Jensen
Objective
This systematic review aimed to determine the differences in the caries-associated microbiota and caries experience among children with type 1 diabetes (T1D) compared to healthy children in observational studies.
Design
Registered in PROPSERO (CRD42024529842), this review involved searching four electronic databases (PubMed, Embase, Scopus and Web of Science) from inception to April 2024. Citation searching was further performed on eligible studies from the database search. Observational studies comparing the cariogenic taxa between paediatric participants (0–18 years of age) with T1D and healthy paediatric participants were included. Caries experience was analysed where available in eligible studies. Outcomes were narratively and tabularly synthesised, and meta-analysis performed where appropriate. All studies were critically appraised using the relevant Joanna Briggs Institute appraisal tool.
Results
425 studies were identified, of which 14 studies were eligible. After assessing 534 citations, 2 additional studies were included. Overall, 16 studies (15 cross-sectional, 1 longitudinal) satisfied the inclusion criteria. Differences in cariogenic bacteria were observed when glycaemic control was considered. Children with T1D and poorer glycaemic control exhibited an increase in Streptococci and Lactobacilli taxa compared to those with improved glycaemic control and healthy children. A small number of studies also found increases in the caries experience with poorer glycaemic control.
Conclusion
Overall, the evidence indicates that children with T1D who have poorer glycaemic control exhibit an increase in cariogenic bacteria. Early evidence suggests that these children also experience more caries. However, further research with improved and longitudinal study designs are required to strengthen the existing evidence.
{"title":"Changes in the caries-associated microbiota and caries experience in children and adolescents with type 1 diabetes: A systematic review and meta-analysis","authors":"Andrew Ta , Sonia Nath , Brianna Poirier , Sneha Sethi , Gabrielle Smart , Emilija Jensen","doi":"10.1016/j.archoralbio.2025.106179","DOIUrl":"10.1016/j.archoralbio.2025.106179","url":null,"abstract":"<div><h3>Objective</h3><div>This systematic review aimed to determine the differences in the caries-associated microbiota and caries experience among children with type 1 diabetes (T1D) compared to healthy children in observational studies.</div></div><div><h3>Design</h3><div>Registered in PROPSERO (CRD42024529842), this review involved searching four electronic databases (PubMed, Embase, Scopus and Web of Science) from inception to April 2024. Citation searching was further performed on eligible studies from the database search. Observational studies comparing the cariogenic taxa between paediatric participants (0–18 years of age) with T1D and healthy paediatric participants were included. Caries experience was analysed where available in eligible studies. Outcomes were narratively and tabularly synthesised, and meta-analysis performed where appropriate. All studies were critically appraised using the relevant Joanna Briggs Institute appraisal tool.</div></div><div><h3>Results</h3><div>425 studies were identified, of which 14 studies were eligible. After assessing 534 citations, 2 additional studies were included. Overall, 16 studies (15 cross-sectional, 1 longitudinal) satisfied the inclusion criteria. Differences in cariogenic bacteria were observed when glycaemic control was considered. Children with T1D and poorer glycaemic control exhibited an increase in <em>Streptococci</em> and <em>Lactobacilli</em> taxa compared to those with improved glycaemic control and healthy children. A small number of studies also found increases in the caries experience with poorer glycaemic control.</div></div><div><h3>Conclusion</h3><div>Overall, the evidence indicates that children with T1D who have poorer glycaemic control exhibit an increase in cariogenic bacteria. Early evidence suggests that these children also experience more caries. However, further research with improved and longitudinal study designs are required to strengthen the existing evidence.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"172 ","pages":"Article 106179"},"PeriodicalIF":2.2,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143070305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-21DOI: 10.1016/j.archoralbio.2025.106180
Na Rae Choi , Hee Sam Na , Hyejung Han , Jin Chung , Yong-Deok Kim
Objective
Antiresorptives cases of adverse reactions to highly effective drugs used to treat skeletal-related events that occur during treatment of osteoporosis or malignancy have been reported in the jaw. To date, there is no clear pathophysiology for medication-related osteonecrosis of the jaw (MRONJ), but the role of inflammation or infection has been proposed as one of several possible pathogenesis theories. This study was designed to investigate the role of infection in the pathogenesis of MRONJ by analyzing the microbial communities observed in the bone samples from MRONJ patients.
Design
Oral microbial samples were collected from 39 MRONJ patients and 45 healthy patients and subjected to next generation sequencing analysis. Alpha diversity, beta diversity, linear discriminant analysis and effect size, and network analysis were applied to compare the microbiome features.
Results
The 10 predominant microbial strains observed in MRONJ patients were Streptococcus spp. S. constellatus, Tannerella forsythia, Parvimonas micra, Granulicatella adiacens, Bacteroidaceae G-1 bacterium HMT 272, Treponema socranskii, T. maltophilum, Pyramidobacter piscolens, and Fretibacterium fastidiosum. Network analysis revealed a significant correlation between T. forsythia and P. gingivalis. T. socranskii was closely related to T. maltophilum, Fretibacterium fastidiosum, and Peptostreptococcaceae nodatum. G. adiacens was closely related to Streptococcus parasanguinis, S. sanguinis, and Prevotella buccae.
Conclusions
In conclusion, this study sheds light on the potential role of infection in the pathogenesis of MRONJ. A distinct microbial profile was identified in MRONJ patients, with notable predominance of S. constellatus, T. forsythia, P. micra and G. adiacens among others. Network analysis further revealed significant correlations between certain microbial strains, suggesting possible synergistic interactions in MRONJ pathogenesis. These findings contribute to a deeper understanding of the complex microbial dynamics underlying MRONJ and may inform future therapeutic strategies aimed at targeting specific bacterial species implicated in this condition.
{"title":"Next-generation sequencing analysis of bacterial species present in the sequestrum of medication-related osteonecrosis of the jaw patients","authors":"Na Rae Choi , Hee Sam Na , Hyejung Han , Jin Chung , Yong-Deok Kim","doi":"10.1016/j.archoralbio.2025.106180","DOIUrl":"10.1016/j.archoralbio.2025.106180","url":null,"abstract":"<div><h3>Objective</h3><div>Antiresorptives cases of adverse reactions to highly effective drugs used to treat skeletal-related events that occur during treatment of osteoporosis or malignancy have been reported in the jaw. To date, there is no clear pathophysiology for medication-related osteonecrosis of the jaw (MRONJ), but the role of inflammation or infection has been proposed as one of several possible pathogenesis theories. This study was designed to investigate the role of infection in the pathogenesis of MRONJ by analyzing the microbial communities observed in the bone samples from MRONJ patients.</div></div><div><h3>Design</h3><div>Oral microbial samples were collected from 39 MRONJ patients and 45 healthy patients and subjected to next generation sequencing analysis. Alpha diversity, beta diversity, linear discriminant analysis and effect size, and network analysis were applied to compare the microbiome features.</div></div><div><h3>Results</h3><div>The 10 predominant microbial strains observed in MRONJ patients were Streptococcus spp. S. constellatus, Tannerella forsythia, Parvimonas micra, Granulicatella adiacens, Bacteroidaceae G-1 bacterium HMT 272, Treponema socranskii, T. maltophilum, Pyramidobacter piscolens, and Fretibacterium fastidiosum. Network analysis revealed a significant correlation between T. forsythia and P. gingivalis. T. socranskii was closely related to T. maltophilum, Fretibacterium fastidiosum, and Peptostreptococcaceae nodatum. G. adiacens was closely related to Streptococcus parasanguinis, S. sanguinis, and Prevotella buccae.</div></div><div><h3>Conclusions</h3><div>In conclusion, this study sheds light on the potential role of infection in the pathogenesis of MRONJ. A distinct microbial profile was identified in MRONJ patients, with notable predominance of S. constellatus, T. forsythia, P. micra and G. adiacens among others. Network analysis further revealed significant correlations between certain microbial strains, suggesting possible synergistic interactions in MRONJ pathogenesis. These findings contribute to a deeper understanding of the complex microbial dynamics underlying MRONJ and may inform future therapeutic strategies aimed at targeting specific bacterial species implicated in this condition.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"172 ","pages":"Article 106180"},"PeriodicalIF":2.2,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143049118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-16DOI: 10.1016/j.archoralbio.2025.106178
Hiroaki Ohki, Satoko Kakino, Tsutomu Iwamoto
Objectives
Transmitted-light plethysmography (TLP) is an objective and non-invasive pulp diagnosis method that has already been validated for applications for incisors. However, there is a demand for TLP use in the molars, it has not yet been established for this application. This study investigated the optimal light source wavelengths for TLP in premolars, to establish a pulp diagnosis system based on measuring pulpal blood flow.
Design
One extracted incisor and one extracted premolar, which were fully developed and healthy, were prepared. The optical properties of model teeth filled with 0–30 % hematocrit contents in the pulp chamber were analyzed at 525, 590, and 625 nm wavelengths. The incident and transmitted light intensity of model teeth were measured to determine the optical density (O.D.) using a prototype plethysmograph (J.Morita) and a spectrometer. The significant differences in O.D. at each wavelength were analyzed using the Kruskal-Wallis test followed by the Steel-Dwass test as a post-hoc test. Light propagation through the teeth was also observed under a microscope.
Results
A statistically significant differences in O.D. were observed among the three wavelengths at all hematocrit concentrations (p < 0.05). The observation of light absorption and scattering in the whole teeth supported the optical measurement results.
Conclusion
The results indicated that the most appropriate wavelengths are 525 nm for incisors and 590 nm for premolars, as it balanced the light transmission through the tooth structure and the sensitivity for detecting changes in blood concentration. Further research is expected to expand the range of applications of TLP in premolars.
{"title":"Evaluation of tooth-specific optical properties for the development of a non-invasive pulp diagnostic system using Transmitted-light plethysmography: An in vitro study","authors":"Hiroaki Ohki, Satoko Kakino, Tsutomu Iwamoto","doi":"10.1016/j.archoralbio.2025.106178","DOIUrl":"10.1016/j.archoralbio.2025.106178","url":null,"abstract":"<div><h3>Objectives</h3><div>Transmitted-light plethysmography (TLP) is an objective and non-invasive pulp diagnosis method that has already been validated for applications for incisors. However, there is a demand for TLP use in the molars, it has not yet been established for this application. This study investigated the optimal light source wavelengths for TLP in premolars, to establish a pulp diagnosis system based on measuring pulpal blood flow.</div></div><div><h3>Design</h3><div>One extracted incisor and one extracted premolar, which were fully developed and healthy, were prepared. The optical properties of model teeth filled with 0–30 % hematocrit contents in the pulp chamber were analyzed at 525, 590, and 625 nm wavelengths. The incident and transmitted light intensity of model teeth were measured to determine the optical density (O.D.) using a prototype plethysmograph (J.Morita) and a spectrometer. The significant differences in O.D. at each wavelength were analyzed using the Kruskal-Wallis test followed by the Steel-Dwass test as a post-hoc test. Light propagation through the teeth was also observed under a microscope.</div></div><div><h3>Results</h3><div>A statistically significant differences in O.D. were observed among the three wavelengths at all hematocrit concentrations (p < 0.05). The observation of light absorption and scattering in the whole teeth supported the optical measurement results.</div></div><div><h3>Conclusion</h3><div>The results indicated that the most appropriate wavelengths are 525 nm for incisors and 590 nm for premolars, as it balanced the light transmission through the tooth structure and the sensitivity for detecting changes in blood concentration. Further research is expected to expand the range of applications of TLP in premolars.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"172 ","pages":"Article 106178"},"PeriodicalIF":2.2,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143049115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-16DOI: 10.1016/j.archoralbio.2025.106177
Ingrid Aquino Reichert Barin , Johnny Carvalho da Silva , Raquel Figuerêdo Ramos , Stella Maris de Freitas Lima , Ana Paula de Castro Cantuária , Poliana Amanda Oliveira Silva , Elaine Maria Guará Lôbo Dantas , Danilo César Mota Martins , Nelson Gomes de Oliveira Júnior , Osmel Fleitas Martínez , Jeeser Alves de Almeida , Marcelo Henrique Soller Ramada , Octávio Luiz Franco , Taia Maria Berto Rezende
Objective
Synoeca-MP is an antimicrobial peptide that belongs to the class of defense peptides, known for their antimicrobial and immunomodulatory properties. To evaluate in vitro the association between synoeca-MP peptide and chlorhexidine, regarding their antimicrobial and antibiofilm activities, saliva stability, effect on tissue repair, bone resorption processes, and mineralized matrix formation.
Design
Initially, the minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and antibiofilm concentration were determined. The synergism and degradation of synoeca-MP and chlorhexidine in human saliva were assessed. Furthermore, biocompatibility was evaluated using MTT assays, hemolytic assays, and proliferation and migration assays of periodontal ligament cells. Finally, bone homeostasis was evaluated through osteoclastogenesis assays, alkaline phosphatase determination, and mineralized matrix formation assay with SaOs-2 and ligament cells.
Results
The antimicrobial and antibiofilm activity against the tested microorganisms was confirmed. Low synergistic concentrations of the synoeca-MP and chlorhexidine combination inhibited tested microorganisms. The association of these molecules remained stable in healthy saliva. Nevertheless, it degraded as the severity of periodontal disease increased. Additionally, lower synergistic concentrations of the combination were not cytotoxic to human cells, promoted the proliferation and migration of ligament cells, inhibited osteoclastogenesis, and increased mineral matrix formation of ligament cells and SaOs-2.
Conclusions
Synoeca-MP and chlorhexidine combination shows potential for oral diseases treatment, as evidenced by its antimicrobial activity, regenerative potential, saliva stability, and bone homeostasis. It may be particularly effective for opportunistic oral infections and in conjunction with mechanical therapy.
{"title":"Antibiofilm, regenerative and bone homeostasis potential of the synergistic association of synoeca-MP peptide with chlorhexidine in oral cavity opportunistic infections","authors":"Ingrid Aquino Reichert Barin , Johnny Carvalho da Silva , Raquel Figuerêdo Ramos , Stella Maris de Freitas Lima , Ana Paula de Castro Cantuária , Poliana Amanda Oliveira Silva , Elaine Maria Guará Lôbo Dantas , Danilo César Mota Martins , Nelson Gomes de Oliveira Júnior , Osmel Fleitas Martínez , Jeeser Alves de Almeida , Marcelo Henrique Soller Ramada , Octávio Luiz Franco , Taia Maria Berto Rezende","doi":"10.1016/j.archoralbio.2025.106177","DOIUrl":"10.1016/j.archoralbio.2025.106177","url":null,"abstract":"<div><h3>Objective</h3><div>Synoeca-MP is an antimicrobial peptide that belongs to the class of defense peptides, known for their antimicrobial and immunomodulatory properties. To evaluate <em>in vitro</em> the association between synoeca-MP peptide and chlorhexidine, regarding their antimicrobial and antibiofilm activities, saliva stability, effect on tissue repair, bone resorption processes, and mineralized matrix formation.</div></div><div><h3>Design</h3><div>Initially, the minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and antibiofilm concentration were determined. The synergism and degradation of synoeca-MP and chlorhexidine in human saliva were assessed. Furthermore, biocompatibility was evaluated using MTT assays, hemolytic assays, and proliferation and migration assays of periodontal ligament cells. Finally, bone homeostasis was evaluated through osteoclastogenesis assays, alkaline phosphatase determination, and mineralized matrix formation assay with SaOs-2 and ligament cells.</div></div><div><h3>Results</h3><div>The antimicrobial and antibiofilm activity against the tested microorganisms was confirmed. Low synergistic concentrations of the synoeca-MP and chlorhexidine combination inhibited tested microorganisms. The association of these molecules remained stable in healthy saliva. Nevertheless, it degraded as the severity of periodontal disease increased. Additionally, lower synergistic concentrations of the combination were not cytotoxic to human cells, promoted the proliferation and migration of ligament cells, inhibited osteoclastogenesis, and increased mineral matrix formation of ligament cells and SaOs-2.</div></div><div><h3>Conclusions</h3><div>Synoeca-MP and chlorhexidine combination shows potential for oral diseases treatment, as evidenced by its antimicrobial activity, regenerative potential, saliva stability, and bone homeostasis. It may be particularly effective for opportunistic oral infections and in conjunction with mechanical therapy.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"172 ","pages":"Article 106177"},"PeriodicalIF":2.2,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143076670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-13DOI: 10.1016/j.archoralbio.2025.106174
Ye Wang , Siyi Zhu , Yaqi Chi , Di Fu , Lin Yao , Mengzhen Ji , Li Jiang , Qi Han , Ling Zou
Objectives
The present study aimed to explore the inhibitory effect of taxifolin (TAX) on Streptococcus mutans (S. mutans) in vitro and evaluated the anti-caries efficacy of TAX in vivo.
Design
The anti-microbial and anti-biofilm properties of TAX were examined on the S. mutans, and the results were preliminarily verified by quantitative real-time PCR. Polarized light microscopy and transverse microradiography were used to detect the effect of TAX on inhibiting enamel demineralization. The effect of TAX on the remineralization of demineralized enamel was analyzed by a microhardness tester, atomic force microscope, and transverse microradiography. The rat dental caries model was constructed to explore the anti-caries effect of TAX in vivo.
Results
The minimum inhibitory concentration of TAX against S. mutans was 1 mg/mL. The 1 mg/mL TAX impeded the biofilm formation, destroyed the biofilm structure, and effectively prevented enamel demineralization caused by S. mutans. Both the 0.5 mg/mL and 1 mg/mL TAX-treated groups exhibited a higher percentage of surface microhardness recovery, along with lower surface roughness, mineral loss, and lesion depth. Additionally, 1 mg/mL TAX demonstrated the ability to inhibit the initiation and progression of caries in rats, while also proving to be biologically safe.
Conclusions
TAX had a significant inhibitory effect on S. mutans, could inhibit enamel demineralization and promote remineralization of demineralized enamel, and showed a promising anti-caries effect in vivo.
{"title":"Preventive effects of taxifolin on dental caries in vitro and in vivo","authors":"Ye Wang , Siyi Zhu , Yaqi Chi , Di Fu , Lin Yao , Mengzhen Ji , Li Jiang , Qi Han , Ling Zou","doi":"10.1016/j.archoralbio.2025.106174","DOIUrl":"10.1016/j.archoralbio.2025.106174","url":null,"abstract":"<div><h3>Objectives</h3><div>The present study aimed to explore the inhibitory effect of taxifolin (TAX) on <em>Streptococcus mutans (S. mutans) in vitro</em> and evaluated the anti-caries efficacy of TAX <em>in vivo</em>.</div></div><div><h3>Design</h3><div>The anti-microbial and anti-biofilm properties of TAX were examined on the <em>S. mutans</em>, and the results were preliminarily verified by quantitative real-time PCR. Polarized light microscopy and transverse microradiography were used to detect the effect of TAX on inhibiting enamel demineralization. The effect of TAX on the remineralization of demineralized enamel was analyzed by a microhardness tester, atomic force microscope, and transverse microradiography. The rat dental caries model was constructed to explore the anti-caries effect of TAX <em>in vivo</em>.</div></div><div><h3>Results</h3><div>The minimum inhibitory concentration of TAX against <em>S. mutans</em> was 1 mg/mL. The 1 mg/mL TAX impeded the biofilm formation, destroyed the biofilm structure, and effectively prevented enamel demineralization caused by <em>S. mutans</em>. Both the 0.5 mg/mL and 1 mg/mL TAX-treated groups exhibited a higher percentage of surface microhardness recovery, along with lower surface roughness, mineral loss, and lesion depth. Additionally, 1 mg/mL TAX demonstrated the ability to inhibit the initiation and progression of caries in rats, while also proving to be biologically safe.</div></div><div><h3>Conclusions</h3><div>TAX had a significant inhibitory effect on <em>S. mutans</em>, could inhibit enamel demineralization and promote remineralization of demineralized enamel, and showed a promising anti-caries effect <em>in vivo</em>.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"172 ","pages":"Article 106174"},"PeriodicalIF":2.2,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143018151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Asiaticoside has the capacity to induce osteogenic differentiation of human periodontal ligament cells (hPDLCs) through Wnt (Wingless-related integration site) signaling. A modified chemical structure (by removing glycoside side chain), referred to as asiatic acid methyl ester (AA1), has been constructed and evaluated for its capacity to induce osteogenic differentiation.
Design
hPDLCs viability was determined by MTT assay. The mRNA expression were analyzed by using quantitative real time PCR. The subcellular localization of β-catenin was demonstrated by immunofluorescence staining. hPDLCs were cultured in osteogenic medium and the deposited calcium was detected by alizarin red staining. The protein expressions were examined by immunofluorescence and western blot.
Results
AA1 at a concentration 50 µM or below was not toxic to hPDLCs whereas asiaticoside had revealed no impact on cell viability at any concentration tested. The results showed that 2.5 µM of AA1 and 100 µM of asiaticoside significantly induced gene expression of osterix and dentin matrix protein1 (DMP1), concomitant with a formation of mineralized nodules. AA1 activated Wnt signaling as shown by an increase of WNT3A expression and nuclear translocation of β-catenin. This activation was effectively inhibited when cells were pretreated with recombinant human Dickkopf1 (rhDKK1). rhDKK1 significantly attenuated the AA1-induced expression of DMP1 at both mRNA and protein level.
Conclusions
These results confirm a common mechanism between asiaticoside and AA1 in fostering osteogenic differentiation of hPDLCs with a higher potency of AA1. In summary, this study has introduced a novel derivative of asiaticoside, which potentially enhance therapeutic effectiveness for periodontal regeneration.
{"title":"Asiatic acid methyl ester, a new asiaticoside derivative, induces osteogenic differentiation of hPDLCs","authors":"Piyamas Sumrejkanchanakij , Atika Resti Fitri , Prasit Pavasant , Suconta Chareonvit , Aye Chan Khine Lin , Supakarn Chamni","doi":"10.1016/j.archoralbio.2025.106175","DOIUrl":"10.1016/j.archoralbio.2025.106175","url":null,"abstract":"<div><h3>Objective</h3><div>Asiaticoside has the capacity to induce osteogenic differentiation of human periodontal ligament cells (hPDLCs) through Wnt (Wingless-related integration site) signaling. A modified chemical structure (by removing glycoside side chain), referred to as asiatic acid methyl ester (AA1), has been constructed and evaluated for its capacity to induce osteogenic differentiation.</div></div><div><h3>Design</h3><div>hPDLCs viability was determined by MTT assay. The mRNA expression were analyzed by using quantitative real time PCR. The subcellular localization of β-catenin was demonstrated by immunofluorescence staining. hPDLCs were cultured in osteogenic medium and the deposited calcium was detected by alizarin red staining. The protein expressions were examined by immunofluorescence and western blot.</div></div><div><h3>Results</h3><div>AA1 at a concentration 50 µM or below was not toxic to hPDLCs whereas asiaticoside had revealed no impact on cell viability at any concentration tested. The results showed that 2.5 µM of AA1 and 100 µM of asiaticoside significantly induced gene expression of osterix and dentin matrix protein1 (<em>DMP1</em>), concomitant with a formation of mineralized nodules. AA1 activated Wnt signaling as shown by an increase of <em>WNT3A</em> expression and nuclear translocation of β-catenin. This activation was effectively inhibited when cells were pretreated with recombinant human Dickkopf1 (rhDKK1). rhDKK1 significantly attenuated the AA1-induced expression of DMP1 at both mRNA and protein level.</div></div><div><h3>Conclusions</h3><div>These results confirm a common mechanism between asiaticoside and AA1 in fostering osteogenic differentiation of hPDLCs with a higher potency of AA1. In summary, this study has introduced a novel derivative of asiaticoside, which potentially enhance therapeutic effectiveness for periodontal regeneration.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"172 ","pages":"Article 106175"},"PeriodicalIF":2.2,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143018148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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