Archives of oral biology最新文献

{"title":"Changes in the craniofacial morphology in sclerostin knockout mice","authors":"Kazutaka Ikeda ,&nbsp;Masato Kaku ,&nbsp;Chao Fan-Yi ,&nbsp;Chisa Shukunami ,&nbsp;Kotaro Tanimoto","doi":"10.1016/j.archoralbio.2025.106492","DOIUrl":"10.1016/j.archoralbio.2025.106492","url":null,"abstract":"<div><h3>Objectives</h3><div>To investigate the influence of lack of sclerostin on craniofacial morphology in sclerostin gene (<em>Sost</em>) knockout (<em>Sost</em>^{<em>Δ26/Δ26</em>}) mice.</div></div><div><h3>Design</h3><div>Micro computed tomography (micro-CT) was performed on both 60-day-old wild-type and <em>Sost</em>^{<em>Δ26/Δ26</em>} mice to analyze changes in craniofacial morphology. Ten anatomical landmarks and eight measurement parameters as well as the total mandibular volume were used to assess craniofacial features. In addition, the femora of both wild-type and <em>Sost</em>^{<em>Δ26/Δ26</em>} mice were examined using micro-CT and histological analysis. Cancellous bone volume, cortical bone volume, and the number of osteoclasts beneath the growth plate in the femora were evaluated.</div></div><div><h3>Results</h3><div>The neurocranium was significantly smaller in the <em>Sost</em>^{<em>Δ26/Δ26</em>} mice than in the wild-type mice, while the length of the angular process of the mandible was significantly greater. The total mandibular volume was significantly larger in the <em>Sost</em>^{<em>Δ26/Δ26</em>} mice than in the wild-type mice. The volume of cancellous bone and cortical bone in the femora were significantly larger in the <em>Sost</em>^{<em>Δ26/Δ26</em>} mice than in the littermate wild-type mice. Histological observation showed no statistically significant difference in the number of osteoclasts present in the femora between wild-type mice and <em>Sost</em>^{<em>Δ26/Δ26</em>} mice.</div></div><div><h3>Conclusions</h3><div>The present study showed a significant decrease in neurocranium and a significant increase in mandibular bone in <em>Sost</em>^{<em>Δ26/Δ26</em>} mice compared to wild-type mice, along with increased volumes of cancellous and cortical bone in the femora.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"183 ","pages":"Article 106492"},"PeriodicalIF":2.1,"publicationDate":"2025-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145881375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Topical sevoflurane enhances periodontal wound healing in a male rat palatal mucosal model","authors":"Gülenay Çolak ,&nbsp;Zeynep Turgut Çankaya ,&nbsp;Çiğdem Elmas ,&nbsp;Gökçe Nur Arık Erol","doi":"10.1016/j.archoralbio.2025.106491","DOIUrl":"10.1016/j.archoralbio.2025.106491","url":null,"abstract":"<div><h3>Objective</h3><div>The aim of this study is to evaluate the effect of topical sevoflurane, which has recently been reported to possess analgesic, anti-inflammatory and antibacterial properties, on secondary wound healing in rat palatal mucosa.</div></div><div><h3>Design</h3><div>Thirty male Wistar Albino rats were randomly divided into three groups: Control, Saline (0.9 % NaCl) and Sevoflurane. Standardized full-thickness excisional wounds (4 mm) were created on the hard palate, and treatment was applied once daily for 14 days. Wound closure, histopathological healing and immunohistochemical expression of vascular endothelial growth factor, interleukin-17A, interleukin-22 and signal transducer and activator of transcription 3 were evaluated on days 5 and 14.</div></div><div><h3>Results</h3><div>On day 5, the sevoflurane group exhibited smaller wound areas (p &lt; 0.05) and greater angiogenesis, epithelialization, and granulation than the control group. On day 14, epithelial integrity and vascular remodeling were most pronounced in the sevoflurane group. IL-22 and pSTAT3 expression levels were higher, while IL-17A expression was lower (p &lt; 0.05). VEGF expression decreased over time, indicating progression toward tissue remodeling.</div></div><div><h3>Conclusions</h3><div>Topical sevoflurane enhances early-stage wound healing by modulating inflammatory and regenerative pathways, promoting angiogenesis, and reducing wound size. These findings suggest that sevoflurane may serve as a novel adjunct for periodontal wound healing.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"183 ","pages":"Article 106491"},"PeriodicalIF":2.1,"publicationDate":"2025-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145879687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative pharmacokinetics of fluoride secretion by major and labial minor salivary glands after oral fluoride intake","authors":"Ligiane Campos Bellose,&nbsp;Leonardo Libardi Pagotto,&nbsp;Deborah Rackel Caldas-da-Rocha,&nbsp;Cinthia Pereira Machado Tabchoury,&nbsp;Antônio Pedro Ricomini-Filho,&nbsp;Jaime Aparecido Cury","doi":"10.1016/j.archoralbio.2025.106488","DOIUrl":"10.1016/j.archoralbio.2025.106488","url":null,"abstract":"<div><h3>Objectives</h3><div>To evaluate whether fluoride secretion by labial minor salivary glands exhibits a pharmacokinetic pattern comparable to that of major salivary glands after oral fluoride intake.</div></div><div><h3>Design</h3><div>In a randomized, blind and crossover study, eight healthy adult participants ingested aqueous NaF solutions containing 5, 10, or 20 mg of fluoride after overnight fasting. Saliva samples were collected at baseline (time 0) and at 5, 15, 30, 60, and 120 min post-ingestion from four sources: labial minor glands (LG), parotid glands (PG), floor of the mouth (FS), and whole saliva (WS). LG saliva and FS were collected using pipette tips under gentle suction, while PG saliva was obtained using the Carlson-Crittenden device and WS was obtained by asking participants to expectorate directly into a 1.5 mL microtube. Fluoride concentrations were determined using a validated micro-method with an inverted fluoride ion-selective electrode. Pharmacokinetic parameters - maximum concentration (Cmax) and area under the curve (AUC) - were calculated. Data were analyzed by two-way ANOVA and linear regression (α=0.05).</div></div><div><h3>Results</h3><div>Fluoride secretion from LG demonstrated a dose-dependent response, with Cmax values of 0.37 ± 0.04, 0.45 ± 0.04, and 0.76 ± 0.19 µg/mL, and AUC values of 29.0 ± 6.2, 33.6 ± 3.4, and 52.7 ± 11.5 µg F/mL <strong>× </strong> min for 5, 10, and 20 mg doses, respectively. These parameters did not differ significantly from those obtained from other salivary glands. A significant linear correlation was observed between the ingested fluoride dose and LG fluoride secretion (R² = 0.6469, p &lt; 0.0001).</div></div><div><h3>Conclusion</h3><div>The pharmacokinetic profile of fluoride secretion in labial minor salivary glands is quantitatively comparable to that of major salivary glands. Their use as an alternative source in fluoride pharmacokinetic studies appears valid, offering a non-invasive and practical sampling method.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"183 ","pages":"Article 106488"},"PeriodicalIF":2.1,"publicationDate":"2025-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145879617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the impact of postnatal maternal separation stress on enamel formation in an experimental murine model","authors":"Júlia I.T. Sousa ,&nbsp;Juliana L. Gonçalves ,&nbsp;Guido A. Marañón-Vásquez ,&nbsp;Roberta D. Leme ,&nbsp;Larissa S. Sales ,&nbsp;Fabrício K. Carvalho ,&nbsp;Alexandra M. Queiroz ,&nbsp;Alice Corrêa Silva-Sousa ,&nbsp;Manoel Damião de Sousa-Neto ,&nbsp;Francisco W.G. Paula-Silva","doi":"10.1016/j.archoralbio.2025.106487","DOIUrl":"10.1016/j.archoralbio.2025.106487","url":null,"abstract":"<div><h3>Background</h3><div>The adverse effects of postnatal maternal separation (MS) on offspring development are well documented; however, its specific impact on dental enamel formation remains inadequately explored. This study examines the effects of maternal-separation–induced toxic stress on dental enamel formation in a murine model. Methods: A total of 24 Wistar rat offspring (<em>Rattus norvegicus</em>) were utilized, divided into a control group (n = 12) and an MS group (n = 12). The MS group underwent 4 h of daily separation from their mothers from postnatal day 2 to day 21, while the control group remained undisturbed with their mothers. Throughout the experimental period, body weight, length, and key developmental milestones (including incisor eruption, eye opening, and ear opening) were monitored. At postnatal day 28, the animals were euthanized, and the incisors were collected for analysis. Incisor photographs were conducted for macroscopic evaluation. Volumetric assessment of the enamel layer was performed using micro-computed tomography (micro-CT) imaging. Microhardness testing quantified enamel resistance, while scanning electron microscopy (SEM) provided insights into the morphology and ultrastructure of incisor enamel. In addition, energy-dispersive X-ray spectroscopy (EDS) was performed to evaluate mineral content and identify possible compositional alterations in dental enamel. Data were statistically analyzed using Student's t-test (α = 0.05). Results: The findings revealed an increase in body weight within the MS group (<em>p</em> &lt; 0.05), while normal growth and developmental milestones exhibited no significant changes (<em>p</em> &gt; 0.05). No macroscopic alterations were observed in the dental enamel of incisors of MS group. SEM analysis of the incisors indicated that the enamel structure of incisors in the MS group maintained an organized arrangement of enamel rods, characterized by densely packed, elongated structures extending from the underlying dentin to enamel surface. Furthermore, structural integrity, microhardness, and mineral composition remained largely unaffected (<em>p</em> &gt; 0.05). Conclusion: Under the given experimental conditions, postnatal maternal separation does not significantly compromise dental enamel formation in rat offspring.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"183 ","pages":"Article 106487"},"PeriodicalIF":2.1,"publicationDate":"2025-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145881674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Odor characteristics and associated bacterial profiles in peri-implantitis using a novel odor measurement device","authors":"Tadahiro Kashiwamura ,&nbsp;Yusuke Taniguchi ,&nbsp;Ryutaro Ito ,&nbsp;Hirofumi Kido ,&nbsp;Kae Kakura ,&nbsp;Nao Suzuki","doi":"10.1016/j.archoralbio.2025.106486","DOIUrl":"10.1016/j.archoralbio.2025.106486","url":null,"abstract":"<div><h3>Objective</h3><div>This study assessed peri-implantitis risk by measuring odor using a novel device capable of identifying odorous components and bacteria specific to peri-implantitis.</div></div><div><h3>Design</h3><div>Implants from eight peri-implantitis patients and superstructures from 20 maintenance patients were evaluated using organoleptic testing and an odor detection device (nose@MEMS, I-PEX, Kyoto, Japan). In the maintenance group, 13 superstructures emitted odor, while 7 did not. Plaque samples were collected from peri-implant sites, and bacterial composition was analyzed via 16S rRNA sequencing.</div></div><div><h3>Results</h3><div>Principal component analysis of odor measurements obtained with the I-PEX system revealed that implants from peri-implantitis patients and odor-positive superstructures during maintenance formed a distribution distinct from odor-negative superstructures. Comparative analysis of bacterial composition between samples in the odor-associated region (Region A) and those including odor-negative superstructures (Region B) showed that Region A had a higher proportion of anaerobic bacteria, while Region B was enriched in commensal oral bacteria. Among 14 bacterial species previously linked to peri-implantitis, <em>Porphyromonas gingivalis</em>, <em>Tannerella forsythia</em>, and <em>Porphyromonas endodontalis</em> were significantly more abundant in Region A, whereas <em>Fretibacterium fastidiosum</em> and <em>Mogibacterium</em> were significantly lower.</div></div><div><h3>Conclusions</h3><div>The odor characteristics of peri-implantitis and odor-positive superstructures were similar and clearly distinguishable from odor-negative counterparts. The shared odor profile was associated with higher proportions of bacterial species previously linked to peri-implantitis. These findings imply that odor analysis using this novel device may provide a promising strategy for implant maintenance and peri-implantitis risk prediction.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"183 ","pages":"Article 106486"},"PeriodicalIF":2.1,"publicationDate":"2025-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145919421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insulin-like growth factor-binding protein 3 regulates collagen production via TGF-β-Smad2/3 pathway and osteogenic differentiation related with phosphorylation of Akt in human periodontal ligament-derived cell line","authors":"Shuxin Wang ,&nbsp;Hiromi Mitarai ,&nbsp;Hiroko Wada ,&nbsp;Ziqing Ran ,&nbsp;Asuka Yuda ,&nbsp;Akira Haraguchi ,&nbsp;Weihao Sun ,&nbsp;Jiawen Lin ,&nbsp;Hidefumi Maeda ,&nbsp;Naohisa Wada","doi":"10.1016/j.archoralbio.2025.106483","DOIUrl":"10.1016/j.archoralbio.2025.106483","url":null,"abstract":"<div><h3>Objective</h3><div>Insulin-like growth factor-binding protein 3 (IGFBP3) is a multifunctional protein involved in various cellular functions. However, the function of IGFBP3 in periodontal ligament (PDL) tissue remains unclear. In this study, we investigated the localization and function of IGFBP3 in PDL tissues and human PDL-derived cell line.</div></div><div><h3>Design</h3><div>Small interfering RNA (siRNA) and recombinant protein of IGFBP3 were used to examine the effect of IGFBP3 in human PDL-derived cell line. mRNA expression was determined by quantitative reverse transcription-polymerase chain reaction. Protein expression was determined using immunohistochemical staining, immunofluorescence staining, and Western blotting analyses. WST-1 and migration assays were used to analyze the effects on proliferation and migration. Collagen production was examined using picrosirius red staining. Calcium nodule formation was examined using Alizarin Red S staining.</div></div><div><h3>Results</h3><div>IGFBP3 was expressed in both mouse PDL tissues and human PDL-derived cell line (2–23 cells). Mechanical stretch and Transforming growth factor-beta 1 (TGF-β1) stimulation upregulated IGFBP3 expression in 2–23 cells. Knockdown of IGFBP3 using siRNA significantly suppressed PDL-related gene expression, collagen production, and inhibited Smad2/3 phosphorylation induced by TGF-β1, while IGFBP3 knockdown enhanced calcium chloride-induced osteogenic differentiation in 2–23 cells and activated Akt phosphorylation. Furthermore, treatment with exogenous rhIGFBP3 showed the opposite trend.</div></div><div><h3>Conclusions</h3><div>IGFBP3 plays a dual role in PDL homeostasis, by promoting collagen production and inhibiting osteogenic differentiation. IGFBP3 is involved in TGF-β-Smad2/3 pathway and related with phosphorylation of Akt, highlighting IGFBP3 as a potential therapeutic target for periodontal regeneration.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"183 ","pages":"Article 106483"},"PeriodicalIF":2.1,"publicationDate":"2025-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145881673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rutin (Vitamin P) attenuates oxidative stress, modulates cytokine profile, and preserves alveolar bone microarchitecture and density in a rat periodontitis model","authors":"Sami Barış Keskin ,&nbsp;Muhammed Mehdi Üremiş ,&nbsp;Yusuf Türköz ,&nbsp;Cüneyt Asım Aral","doi":"10.1016/j.archoralbio.2025.106485","DOIUrl":"10.1016/j.archoralbio.2025.106485","url":null,"abstract":"<div><h3>Objectives</h3><div>To evaluate the anti-inflammatory and antioxidant effects of rutin in experimental periodontitis.</div></div><div><h3>Design</h3><div>Wistar Albino rats were divided into four groups (n = 8 per group): 1) Healthy Control (HC), 2) Periodontitis (P), 3) P + Rutin 50 mg/kg (PR-50), and 4) P + Rutin 100 mg/kg (PR-100). Rutin was administered orally throughout the 14-day experimental period. Gingival levels of interleukin-1 beta (IL-1β), interleukin-10 (IL-10), glutathione peroxidase (GPX), malondialdehyde (MDA), and superoxide dismutase (SOD) and were measured using enzyme-linked immunosorbent assay, while oxidative stress index (OSI), total oxidant (TOS), and antioxidant status (TAS) were analysed spectrophotometrically. Alveolar bone was assessed by micro-computed tomography, including linear (ABL), volumetric (BV/TV), microarchitectural (BS/BV, Tb.Th, Tb.N, Tb.Sp), and densitometric (GV, HU, BMD) parameters.</div></div><div><h3>Results</h3><div>IL-1β/IL-10 ratio was lower, and IL-10 and GPX levels were higher in PR-100 group than in P group (p &lt; 0.05). SOD levels were higher in HC and PR-100 groups than in P group (p &lt; 0.001). OSI was highest in P (p &lt; 0.001). TOS was higher in P versus HC, whereas TAS was lower in PR-50 versus HC (p &lt; 0.05). ABL and BV/TV analyses showed significant bone preservation in both rutin groups compared to P (p &lt; 0.01). Microstructural and densitometric indices further confirmed less alveolar bone deterioration in rutin-treated rats.</div></div><div><h3>Conclusion</h3><div>Rutin exerts dose-dependent anti-inflammatory, antioxidant, and bone-protective effects in experimental periodontitis, suggesting its potential as an adjunctive therapeutic agent.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"183 ","pages":"Article 106485"},"PeriodicalIF":2.1,"publicationDate":"2025-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145771915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Submandibular saliva from male rats modulates osteogenic differentiation of bone marrow progenitor cells In Vitro","authors":"Gastón R. Troncoso ,&nbsp;María V. Gangoiti ,&nbsp;Javier Fernández-Solari ,&nbsp;Claudia E. Mohn ,&nbsp;María S. Molinuevo","doi":"10.1016/j.archoralbio.2025.106484","DOIUrl":"10.1016/j.archoralbio.2025.106484","url":null,"abstract":"<div><h3>Objective</h3><div>To investigate the effect of submandibular saliva from male rats on the proliferation and osteoblastic differentiation of bone marrow progenitor cells.</div></div><div><h3>Design</h3><div>Saliva was collected and pooled from adult male rats, and the total protein content was measured. Bone marrow progenitor cells (BMPC) from adult male rats were incubated with varying concentrations of salivary protein (ranging from 0 to 30 µg/mL) to assess cell proliferation. Osteoblastic differentiation was evaluated by measuring alkaline phosphatase activity, collagen type 1 production, mineral nodule formation, and molecular markers of differentiation after 7, 15, or 21 days of saliva exposure in a differentiating culture medium. The pro-inflammatory effects of saliva on BMPC were assessed by measuring tumor necrosis factor-alpha, interleukin 1 beta, prostaglandin E2, and matrix metalloproteinases 2 and 9.</div></div><div><h3>Results</h3><div>Low saliva concentration stimulated BMPC, promoting a pro-secretory and proliferative state. In contrast, higher concentration inhibited both processes under basal conditions. Furthermore, in osteogenic medium, saliva decreased alkaline phosphatase activity, collagen-1 production, and matrix mineralization in BMPC, demonstrating a dose- and time-dependent effect. Saliva also increased the secretion of interleukin 1β, tumor necrosis factor α, prostaglandin E2, and metalloproteinase activity in these cells, which inhibited osteoblastic differentiation.</div></div><div><h3>Conclusion</h3><div>Submandibular saliva has a biphasic effect on the osteogenic commitment of bone marrow progenitor cells, depending on the concentration and duration of exposure. This finding underscores the active role of saliva in the repair and regeneration of tooth sockets.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"182 ","pages":"Article 106484"},"PeriodicalIF":2.1,"publicationDate":"2025-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145776683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and eradication of mixed biofilms of Streptococcus mutans and Candida albicans using naringin: In vitro and in silico investigations","authors":"Chevuru Sai Shreya Reddy ,&nbsp;Lekha Sree Venkatesan ,&nbsp;Dhanraj Ganapathy,&nbsp;Palanivel Sathishkumar","doi":"10.1016/j.archoralbio.2025.106482","DOIUrl":"10.1016/j.archoralbio.2025.106482","url":null,"abstract":"<div><h3>Objective</h3><div>To identify a potential natural therapeutic agent to treat the mixed biofilms of <em>Streptococcus mutans</em> and <em>Candida albicans</em>, thereby preventing dental caries.</div></div><div><h3>Design</h3><div>The coexisting <em>S. mutans</em> and <em>C. albicans</em> was isolated from dental caries. Antimicrobial activity of various natural components was assessed against <em>S. mutans</em> and <em>C. albicans</em>. Antibiofilm efficacy of naringin was assessed against the biofilm of mixed <em>S. mutans</em> and <em>C. albicans</em>. The cell viability in mixed biofilm was determined using MTT assay and CLSM investigations. The molecular docking analysis of naringin with secreted aspartic proteinase (SAP2) of <em>C. albicans</em> and glucosyltransferase-I (GtfB) of <em>S. mutans</em> was performed using AutoDock and PyMOL tools. The biocompatibility of naringin was determined on human RBCs and HGF cells.</div></div><div><h3>Results</h3><div>The natural components such as curcumin, naringin, quercetin, morin, rutin, and hesperetin shows the antimicrobial effects against the clinical isolates <em>S. mutans</em> and <em>C. albicans</em>. Among these, naringin exhibits the significant antimicrobial effect against <em>S. mutans</em> (MIC:183 ± 5.70 µM), <em>C. albicans</em> (MIC:196 ± 0.00 µM), and their mixed culture (MIC:200 ± 10.00 µM). The MTT and CLSM results indicates that 2xMIC (400 µM) of naringin demonstrates the potential eradication of mature biofilm of mixed <em>S. mutans</em> and <em>C. albicans</em>. Naringin establishes the strong binding interaction with enzymes GtfB of <em>S. mutans</em> and SAP2 of <em>C. albicans</em>. The IC₅₀ value of naringin towards HGF cells was noted as 711.5 µM.</div></div><div><h3>Conclusions</h3><div>The flavonoid naringin demonstrates the effective and safe therapeutic potential to treat coexisting <em>S. mutans</em> and <em>C. albicans</em> biofilm virulence to prevent the dental caries.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"183 ","pages":"Article 106482"},"PeriodicalIF":2.1,"publicationDate":"2025-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145799797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the relationship between gut microbiota-metabolite axis and Jiawei Danxuan Koukang’s therapeutic effects in male rats with oral submucous fibrosis: A multi-omics analysis","authors":"Chenwei Wang ,&nbsp;Yuzhe Dai ,&nbsp;Yao Ye ,&nbsp;Qianqi Zeng ,&nbsp;Jin Tan","doi":"10.1016/j.archoralbio.2025.106480","DOIUrl":"10.1016/j.archoralbio.2025.106480","url":null,"abstract":"<div><h3>Objective</h3><div>This work aims to explore how Jiawei Danxuan Koukang (JDK) ameliorates Oral Submucous Fibrosis (OSF) via regulating the gut microbiota and its metabolites.</div></div><div><h3>Design</h3><div>We established an OSF rat model via oral mucosal arecoline injection. Rats were randomly divided into five groups: control, model, lycopene-treated, quercetin-treated, and JDK-treated. After intervention, we analyzed: serum metabolites by Liquid Chromatography-Mass Spectrometry -based untargeted metabolomics; gut microbiota profiling via 16S rDNA sequencing; oral mucosal histopathology and fibrosis using hematoxylin-eosin and Masson trichrome staining; expressions of fibrosis-related proteins (Transforming Growth Factor-β1 (TGF-β1), Collagen Type I Alpha 1 Chain (COL1A1)) by western blot; and cytokines (Interleukin-1β, Interleukin-6, Interleukin-10) in serum, oral and colon tissues via enzyme-linked immunosorbent assay.</div></div><div><h3>Results</h3><div>In the OSF model group, 120 serum metabolites were upregulated and 39 were down-regulated. When comparing the JDK group with the model group, 83 metabolites were upregulated and 132 were downregulated in the JDK group. Specifically, JDK targeted key metabolites: prostaglandins, leukotrienes, lysophosphatidylcholine, and 4-hydroxyproline. JDK also regulated two critical metabolic pathways: gly-cine-serine-threonine metabolism and tryptophan metabolism. JDK reduces the pro-inflammatory Ruminococcus and restores the butyrate-producing Lachnospiraceae_NK4A136_group. JDK downregulated Interleukin-1β and Interleukin-6 levels systemically and locally, concurrently increasing Interleukin-10, and reduced the expression of fibrosis-related proteins (TGF-β1, COL1A1) in the oral mucosa.</div></div><div><h3>Conclusions</h3><div>JDK alleviates OSF by normalizing inflammation and fibrosis-related metabolic pathways, linking gut microbiota remodeling to metabolic homeostasis.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"182 ","pages":"Article 106480"},"PeriodicalIF":2.1,"publicationDate":"2025-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145770178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}