Pub Date : 2024-12-01DOI: 10.1016/j.arcmed.2024.103138
Sergio Papier , Fiamma Di Biase , Julieta Quaglia
{"title":"Reproductive medicine: The future is now","authors":"Sergio Papier , Fiamma Di Biase , Julieta Quaglia","doi":"10.1016/j.arcmed.2024.103138","DOIUrl":"10.1016/j.arcmed.2024.103138","url":null,"abstract":"","PeriodicalId":8318,"journal":{"name":"Archives of Medical Research","volume":"55 8","pages":"Article 103138"},"PeriodicalIF":4.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142746045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-01DOI: 10.1016/j.arcmed.2024.103136
Taylor Badger , Emma Johns , Teresa Wilson , Kathleen Groesch , Paula Diaz-Sylvester , Erin Bauer , Albert Botchway , Melanie Nielsen , Julio Ricardo Loret de Mola , Beau Batton
Objective
To compare in-hospital and neurodevelopmental (ND) outcomes between very preterm infants conceived spontaneously and those conceived utilizing in vitro fertilization (IVF).
Study Design
A 10-year retrospective study of a large regional, mixed urban and rural population was conducted. IVF conceived infants born <32 0/7 weeks gestational age (GA) were matched 1:2 to control patients who did not undergo any documented assisted reproductive technology (ART) based on GA, birth weight and gender. In-hospital and ND data were analyzed.
Results
There were 35,712 live births, including 755 (2.1%) IVF-conceived births (519 [68.7%] full term, 236 [31.3%] preterm, 33 born <32 0/7 weeks GA [14.0%] – including 27 cared for in the HSHS St. John's Hospital NICU with long-term ND data). When compared to 54 controls, these 27 very preterm IVF-conceived infants were more likely to be born to older women (OR 1.21 [1.07–1.35], p = 0.001), but other demographic data (i.e., including primigravida status, length of hospital stay, use of antenatal corticosteroids, mode of delivery, rural residency, etc.) were similar. Compared to other very preterm neonates, IVF conceived very preterm infants were less likely to receive surfactant therapy (9 [33%] vs. 34 [63%], p = 0.104). Other in-hospital outcomes were similar. Data for ND outcomes through 24 months (non-matched data due to loss to follow-up) were also similar between cohorts.
Conclusion
Infants born very preterm after IVF conception have similar in-hospital and ND outcomes when compared to other very preterm infants. This is reassuring information that can be shared with individuals considering IVF conception.
{"title":"Developmental outcomes of very preterm infants conceived through in vitro fertilization","authors":"Taylor Badger , Emma Johns , Teresa Wilson , Kathleen Groesch , Paula Diaz-Sylvester , Erin Bauer , Albert Botchway , Melanie Nielsen , Julio Ricardo Loret de Mola , Beau Batton","doi":"10.1016/j.arcmed.2024.103136","DOIUrl":"10.1016/j.arcmed.2024.103136","url":null,"abstract":"<div><h3>Objective</h3><div>To compare in-hospital and neurodevelopmental (ND) outcomes between very preterm infants conceived spontaneously and those conceived utilizing <em>in vitro</em> fertilization (IVF).</div></div><div><h3>Study Design</h3><div>A 10-year retrospective study of a large regional, mixed urban and rural population was conducted. IVF conceived infants born <32 0/7 weeks gestational age (GA) were matched 1:2 to control patients who did not undergo any documented assisted reproductive technology (ART) based on GA, birth weight and gender. In-hospital and ND data were analyzed.</div></div><div><h3>Results</h3><div>There were 35,712 live births, including 755 (2.1%) IVF-conceived births (519 [68.7%] full term, 236 [31.3%] preterm, 33 born <32 0/7 weeks GA [14.0%] – including 27 cared for in the HSHS St. John's Hospital NICU with long-term ND data). When compared to 54 controls, these 27 very preterm IVF-conceived infants were more likely to be born to older women (OR 1.21 [1.07–1.35], <em>p</em> = 0.001), but other demographic data (i.e., including primigravida status, length of hospital stay, use of antenatal corticosteroids, mode of delivery, rural residency, etc.) were similar. Compared to other very preterm neonates, IVF conceived very preterm infants were less likely to receive surfactant therapy (9 [33%] vs. 34 [63%], <em>p</em> = 0.104). Other in-hospital outcomes were similar. Data for ND outcomes through 24 months (non-matched data due to loss to follow-up) were also similar between cohorts.</div></div><div><h3>Conclusion</h3><div>Infants born very preterm after IVF conception have similar in-hospital and ND outcomes when compared to other very preterm infants. This is reassuring information that can be shared with individuals considering IVF conception.</div></div>","PeriodicalId":8318,"journal":{"name":"Archives of Medical Research","volume":"55 8","pages":"Article 103136"},"PeriodicalIF":4.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142746044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Due to increased exposure to health hazards, patients with cancer are at a higher risk of developing infective endocarditis (IE), which in turn maximizes their risk of poor outcomes. This study aimed to analyze IE events in patients with cancer and matched controls.
Methods
We conducted a retrospective case-control study in four third-level centers in Mexico City between 2006 and 2022. Patients with IE were identified; cases (patients with cancer) were matched in a 1:2 ratio with controls (patients without cancer). Baseline characteristics, factors associated with IE, and outcomes were assessed in both groups.
Results
A total of 108 patients were included (36 cases and 72 controls). After logistic regression analysis, the risk factors independently associated with IE in cases were obesity (adjusted odds ratio [aOR] 9.03, 95 % CI 1.5–51.8), surgery within six months before IE (aOR 6.23, 95 % CI 1.8–21.5), and invasive procedures within six months prior to IE (aOR 3.89, 95 % CI 1.15–13.1). Healthcare-associated IE was more common in these cases, as were systemic embolic episodes. Subjects with S. aureus IE were more prone to experience systemic embolization. There were no differences in mortality between the groups.
Conclusion
In this study, risk factors associated with the healthcare environment were more frequent in patients with IE and cancer. S. aureus was a common culprit and was associated with systemic embolization.
由于暴露于健康危害因素的增加，癌症患者发生感染性心内膜炎（IE）的风险更高，这反过来又使其预后不良的风险最大化。本研究旨在分析癌症患者和对照组的IE事件。方法2006 - 2022年在墨西哥城4个三级中心进行回顾性病例对照研究。确定IE患者；病例（癌症患者）与对照组（非癌症患者）按1:2的比例配对。对两组的基线特征、与IE相关的因素和结果进行评估。结果共纳入108例患者，其中病例36例，对照组72例。经logistic回归分析，与IE病例独立相关的危险因素为肥胖（调整比值比[aOR] 9.03, 95% CI 1.5-51.8）、IE前6个月内的手术（aOR 6.23, 95% CI 1.8-21.5）和IE前6个月内的有创手术（aOR 3.89, 95% CI 1.15-13.1）。在这些病例中，与医疗保健相关的IE更为常见，系统性栓塞发作也是如此。金黄色葡萄球菌IE患者更容易出现全身栓塞。两组之间的死亡率没有差异。结论与卫生环境相关的危险因素在IE合并癌症患者中更为常见。金黄色葡萄球菌是常见的罪魁祸首，并与全身栓塞有关。
{"title":"Clinical characteristics and outcomes of infective endocarditis in patients with cancer: A multicenter case-control study","authors":"Gustavo Mendez-Hirata , Frida Rivera-Buendía , Eduardo Viveros-Rentería , Eric Ochoa-Hein , Patricia Cornejo-Juárez , Manlio Márquez , José Ramón Hidalgo-Dura , Yoana Leyva-López , Diana Vilar-Compte","doi":"10.1016/j.arcmed.2024.103123","DOIUrl":"10.1016/j.arcmed.2024.103123","url":null,"abstract":"<div><h3>Background</h3><div>Due to increased exposure to health hazards, patients with cancer are at a higher risk of developing infective endocarditis (IE), which in turn maximizes their risk of poor outcomes. This study aimed to analyze IE events in patients with cancer and matched controls.</div></div><div><h3>Methods</h3><div>We conducted a retrospective case-control study in four third-level centers in Mexico City between 2006 and 2022. Patients with IE were identified; cases (patients with cancer) were matched in a 1:2 ratio with controls (patients without cancer). Baseline characteristics, factors associated with IE, and outcomes were assessed in both groups.</div></div><div><h3>Results</h3><div>A total of 108 patients were included (36 cases and 72 controls). After logistic regression analysis, the risk factors independently associated with IE in cases were obesity (adjusted odds ratio [aOR] 9.03, 95 % CI 1.5–51.8), surgery within six months before IE (aOR 6.23, 95 % CI 1.8–21.5), and invasive procedures within six months prior to IE (aOR 3.89, 95 % CI 1.15–13.1). Healthcare-associated IE was more common in these cases, as were systemic embolic episodes. Subjects with <em>S. aureus</em> IE were more prone to experience systemic embolization. There were no differences in mortality between the groups.</div></div><div><h3>Conclusion</h3><div>In this study, risk factors associated with the healthcare environment were more frequent in patients with IE and cancer. <em>S. aureus</em> was a common culprit and was associated with systemic embolization.</div></div>","PeriodicalId":8318,"journal":{"name":"Archives of Medical Research","volume":"56 3","pages":"Article 103123"},"PeriodicalIF":4.7,"publicationDate":"2024-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142744274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-29DOI: 10.1016/j.arcmed.2024.103126
David A. Forero-Peña , Fhabián S. Carrión-Nessi , Óscar D. Omaña-Ávila , Daniela L. Mendoza-Millán , Augusto Moncada-Ortega , María E. Landaeta
The humanitarian crisis in Venezuela has become an unprecedented regional emergency. Over the past decade, Venezuela's political and economic collapse has eroded the country's healthcare infrastructure and threatened the public health of the population. Shortages of medicines and health supplies, disruptions in basic services, and the emigration of health workers during this humanitarian crisis have affected access to care throughout the country, as well as the capacity of emergency services and outbreak response. During the early waves of the epidemic, COVID-19 collapsed the Venezuelan health system, leaving sentinel centers without inpatient beds and ICU space. Home-based care alternatives quickly emerged, not only for mild to moderate but also for severe and critical COVID-19 cases. Here we discuss the impact of the pandemic on the Venezuelan health system, the emergence of home-based care alternatives for severe and critical COVID-19 cases, as well as their costs and challenges, and finally, their advantages and disadvantages.
{"title":"COVID-19 in Venezuela: Costs and challenges of management severe cases at home in a crisis setting","authors":"David A. Forero-Peña , Fhabián S. Carrión-Nessi , Óscar D. Omaña-Ávila , Daniela L. Mendoza-Millán , Augusto Moncada-Ortega , María E. Landaeta","doi":"10.1016/j.arcmed.2024.103126","DOIUrl":"10.1016/j.arcmed.2024.103126","url":null,"abstract":"<div><div>The humanitarian crisis in Venezuela has become an unprecedented regional emergency. Over the past decade, Venezuela's political and economic collapse has eroded the country's healthcare infrastructure and threatened the public health of the population. Shortages of medicines and health supplies, disruptions in basic services, and the emigration of health workers during this humanitarian crisis have affected access to care throughout the country, as well as the capacity of emergency services and outbreak response. During the early waves of the epidemic, COVID-19 collapsed the Venezuelan health system, leaving sentinel centers without inpatient beds and ICU space. Home-based care alternatives quickly emerged, not only for mild to moderate but also for severe and critical COVID-19 cases. Here we discuss the impact of the pandemic on the Venezuelan health system, the emergence of home-based care alternatives for severe and critical COVID-19 cases, as well as their costs and challenges, and finally, their advantages and disadvantages.</div></div>","PeriodicalId":8318,"journal":{"name":"Archives of Medical Research","volume":"56 3","pages":"Article 103126"},"PeriodicalIF":4.7,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142744479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Interstitial fibrosis is the primary determinant of the progression of chronic kidney disease (CKD), and noninvasive identification of interstitial fibrosis is a major challenge. We aimed to explore the diagnostic value of secreted protein acidic and rich in cysteine (SPARC) in serum and urine in kidney fibrosis.
Methods
Single-cell transcriptome analysis was used to measure SPARC expression in healthy reference kidneys and those of patients with CKD. A total of 674 patients with CKD who underwent renal biopsy served as the training cohort (n = 322) and the validation cohort (n = 352). Serum and urinary SPARC levels were measured at the time of kidney biopsy. In vivo and in vitro models of kidney fibrosis were also used to confirm the role of SPARC.
Results
Increased SPARC expression was detected in kidney fibrosis tissues. Higher serum SPARC levels were associated with increased severity of kidney fibrosis. Moreover, the area under the receiver operating characteristic curve (AUC-ROC) (AUC 0.86) was greater for the serum SPARC level than for the urinary SPARC level and estimated glomerular filtration rate (eGFR). The combination of the serum and urinary SPARC levels and eGFR increased the AUC-ROC for predicting kidney fibrosis from 0.86 to 0.90. The diagnostic performance of serum or urinary SPARC levels was consistent in the validation cohort. In vivo and in vitro models of kidney fibrosis also confirmed the upregulation of SPARC expression.
Conclusions
Serum and urinary SPARC levels may be potential biomarkers for kidney fibrosis and may be useful for noninvasive diagnosis.
{"title":"Elevated serum and urinary secreted protein acidic and rich in cysteine levels are novel biomarkers of kidney fibrosis severity","authors":"Yang-yang Niu , Ying Yu , Wen-qian Zhou , Xiao-qin Zhang , Sai-ya Zhu , Ying-ying Zhang , Xin Li , Hong-ping Shan , Jian-ying Niu , Tian-jun Guan , Chen Yu","doi":"10.1016/j.arcmed.2024.103125","DOIUrl":"10.1016/j.arcmed.2024.103125","url":null,"abstract":"<div><h3>Background</h3><div>Interstitial fibrosis is the primary determinant of the progression of chronic kidney disease (CKD), and noninvasive identification of interstitial fibrosis is a major challenge. We aimed to explore the diagnostic value of secreted protein acidic and rich in cysteine (SPARC) in serum and urine in kidney fibrosis.</div></div><div><h3>Methods</h3><div>Single-cell transcriptome analysis was used to measure SPARC expression in healthy reference kidneys and those of patients with CKD. A total of 674 patients with CKD who underwent renal biopsy served as the training cohort (<em>n</em> = 322) and the validation cohort (<em>n</em> = 352). Serum and urinary SPARC levels were measured at the time of kidney biopsy. In vivo and in vitro models of kidney fibrosis were also used to confirm the role of SPARC.</div></div><div><h3>Results</h3><div>Increased SPARC expression was detected in kidney fibrosis tissues. Higher serum SPARC levels were associated with increased severity of kidney fibrosis. Moreover, the area under the receiver operating characteristic curve (AUC-ROC) (AUC 0.86) was greater for the serum SPARC level than for the urinary SPARC level and estimated glomerular filtration rate (eGFR). The combination of the serum and urinary SPARC levels and eGFR increased the AUC-ROC for predicting kidney fibrosis from 0.86 to 0.90. The diagnostic performance of serum or urinary SPARC levels was consistent in the validation cohort. In vivo and in vitro models of kidney fibrosis also confirmed the upregulation of SPARC expression.</div></div><div><h3>Conclusions</h3><div>Serum and urinary SPARC levels may be potential biomarkers for kidney fibrosis and may be useful for noninvasive diagnosis.</div></div>","PeriodicalId":8318,"journal":{"name":"Archives of Medical Research","volume":"56 3","pages":"Article 103125"},"PeriodicalIF":4.7,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142722410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-27DOI: 10.1016/j.arcmed.2024.103137
Julio Ricardo Loret de Mola
Laparoscopic Robot-assisted surgery is one of the most promising and rapidly developing surgical advancements of the twenty-first century with a potential to make significant contributions to reproductive medicine and preservation of fertility. Presently, laparoscopic robotic assisted surgery is used for various benign and malignant gynecological procedures, including fertility enhancing procedures. Laparoscopic Robot-assisted surgery is superior to traditional open procedures with regards to post-surgical hospital stay and blood loss; however, the difference is comparable to laparoscopic surgery. Regarding operative times, the results have been inconsistent due to variations in surgeons’ experience. The primary drawbacks of robotic systems are their high installation and maintenance costs and historical lack of tactile feedback; however, this has been overcome by the most recent evolution of robotic systems. Along with the major advances in cancer therapy, the number of female cancer survivors of reproductive age has dramatically increased. Consequently, fertility preservation and fertility enhancement have gained more emphasis in reproductive surgery in the last decade. A broad range of surgical procedures such as tubal reanastomosis, myomectomy, treatment of deep infiltrating endometriosis, ovarian transposition, radical trachelectomy, and ovarian transplantation has been introduced to restore or preserve fertility using Laparoscopic Robot-assisted surgery. In this article, we aim to present the current applications, advantages, and disadvantages of Laparoscopic Robot-assisted surgical technology in the field of reproductive surgery, including the more recent advances of Artificial Intelligence (AI) in the field.
{"title":"Laparoscopic robotic assisted surgery in reproductive medicine","authors":"Julio Ricardo Loret de Mola","doi":"10.1016/j.arcmed.2024.103137","DOIUrl":"10.1016/j.arcmed.2024.103137","url":null,"abstract":"<div><div>Laparoscopic Robot-assisted surgery is one of the most promising and rapidly developing surgical advancements of the twenty-first century with a potential to make significant contributions to reproductive medicine and preservation of fertility. Presently, laparoscopic robotic assisted surgery is used for various benign and malignant gynecological procedures, including fertility enhancing procedures. Laparoscopic Robot-assisted surgery is superior to traditional open procedures with regards to post-surgical hospital stay and blood loss; however, the difference is comparable to laparoscopic surgery. Regarding operative times, the results have been inconsistent due to variations in surgeons’ experience. The primary drawbacks of robotic systems are their high installation and maintenance costs and historical lack of tactile feedback; however, this has been overcome by the most recent evolution of robotic systems. Along with the major advances in cancer therapy, the number of female cancer survivors of reproductive age has dramatically increased. Consequently, fertility preservation and fertility enhancement have gained more emphasis in reproductive surgery in the last decade. A broad range of surgical procedures such as tubal reanastomosis, myomectomy, treatment of deep infiltrating endometriosis, ovarian transposition, radical trachelectomy, and ovarian transplantation has been introduced to restore or preserve fertility using Laparoscopic Robot-assisted surgery. In this article, we aim to present the current applications, advantages, and disadvantages of Laparoscopic Robot-assisted surgical technology in the field of reproductive surgery, including the more recent advances of Artificial Intelligence (AI) in the field.</div></div>","PeriodicalId":8318,"journal":{"name":"Archives of Medical Research","volume":"55 8","pages":"Article 103137"},"PeriodicalIF":4.7,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142723624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-26DOI: 10.1016/j.arcmed.2024.103130
Alfredo Góngora , William V. Holt , Jaime Gosálvez
The purpose of this article is to analyze in detail the advantages and disadvantages of sperm cryopreservation, focusing on the cellular and molecular changes that occur during these processes. The main issue is the cellular damage caused by ice crystal formation and osmotic imbalance, along with other secondary effects such as sperm motility and viability, as well as the acrosome reaction or oxidative stress. Another important aspect is the examination of how chromatin structure and DNA integrity affect sperm. Biochemical changes affecting enzyme activity and protein stability have also been analyzed. Finally, the article highlights emerging technologies aimed at reducing the damage caused by sperm cryopreservation, as well as the potential benefits of biobanks as an essential resource for addressing male infertility.
本文旨在详细分析精子冷冻保存的优缺点，重点关注冷冻过程中发生的细胞和分子变化。主要问题是冰晶形成和渗透失衡造成的细胞损伤，以及其他次要影响，如精子活力和存活率，以及顶体反应或氧化应激。另一个重要方面是研究染色质结构和 DNA 的完整性如何影响精子。此外，还分析了影响酶活性和蛋白质稳定性的生化变化。最后，文章重点介绍了旨在减少精子冷冻造成的损害的新兴技术，以及生物库作为解决男性不育问题的重要资源的潜在益处。
{"title":"Sperm human biobanking: An overview","authors":"Alfredo Góngora , William V. Holt , Jaime Gosálvez","doi":"10.1016/j.arcmed.2024.103130","DOIUrl":"10.1016/j.arcmed.2024.103130","url":null,"abstract":"<div><div>The purpose of this article is to analyze in detail the advantages and disadvantages of sperm cryopreservation, focusing on the cellular and molecular changes that occur during these processes. The main issue is the cellular damage caused by ice crystal formation and osmotic imbalance, along with other secondary effects such as sperm motility and viability, as well as the acrosome reaction or oxidative stress. Another important aspect is the examination of how chromatin structure and DNA integrity affect sperm. Biochemical changes affecting enzyme activity and protein stability have also been analyzed. Finally, the article highlights emerging technologies aimed at reducing the damage caused by sperm cryopreservation, as well as the potential benefits of biobanks as an essential resource for addressing male infertility.</div></div>","PeriodicalId":8318,"journal":{"name":"Archives of Medical Research","volume":"55 8","pages":"Article 103130"},"PeriodicalIF":4.7,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142723625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-26DOI: 10.1016/j.arcmed.2024.103127
Marlene López-Sánchez , Aleida Bautista-Santos , María del Pilar Milke-García , Aldo Allende-López , Rosalba Moreno-Alcántar , Segundo Morán
Background
Malnutrition in patients with liver cirrhosis (LC) and/or hepatocellular carcinoma (HCC) has been associated with adverse outcomes. However, there is little information on the incidence of HCC during the compensated phase of LC in relation to the nutritional status.
Aim
To evaluate the association between the incidence of HCC in compensated LC and their nutritional status.
Methods
Patients with compensated liver cirrhosis with no previous history of ascites, hepatic encephalopathy, or variceal bleeding attending the Gastroenterology outpatient service at Centro Medico Nacional Siglo XXI were included in a prospective cohort. Clinical and nutritional parameters were collected, including the Royal Free Hospital Subjective Global Assessment (RFH-SGA) as an indicator of protein-calorie malnutrition and the triceps skinfold thickness, which classified patients as having normal subcutaneous adipose tissue (SAT), above average SAT, and below average SAT. Follow-up was censored at the time of HCC diagnosis or LC decompensation.
Results
About 31/187 (16.0 %) and 22/187 (11.8 %) patients were categorized as having above- or below-average SAT at baseline, respectively. 10/187 patients (5.3 %) developed HCC during the compensated phase of LC at a median of 22 months (IQR: 10.0–36.75). A higher risk of HCC was observed in subjects below average SAT (HR: 4.064, CI 95 %: 1.012–16.317, p = 0.048). After adjusting the Cox models for age and α-fetoprotein at baseline, the statistical significance of the association between SAT and HCC was not modified.
Conclusion
These results suggest that decreased SAT may precede the diagnosis of HCC in compensated LC.
{"title":"Nutritional status and incidence of hepatocellular carcinoma in patients with compensated liver cirrhosis","authors":"Marlene López-Sánchez , Aleida Bautista-Santos , María del Pilar Milke-García , Aldo Allende-López , Rosalba Moreno-Alcántar , Segundo Morán","doi":"10.1016/j.arcmed.2024.103127","DOIUrl":"10.1016/j.arcmed.2024.103127","url":null,"abstract":"<div><h3>Background</h3><div>Malnutrition in patients with liver cirrhosis (LC) and/or hepatocellular carcinoma (HCC) has been associated with adverse outcomes. However, there is little information on the incidence of HCC during the compensated phase of LC in relation to the nutritional status.</div></div><div><h3>Aim</h3><div>To evaluate the association between the incidence of HCC in compensated LC and their nutritional status.</div></div><div><h3>Methods</h3><div>Patients with compensated liver cirrhosis with no previous history of ascites, hepatic encephalopathy, or variceal bleeding attending the Gastroenterology outpatient service at Centro Medico Nacional Siglo XXI were included in a prospective cohort. Clinical and nutritional parameters were collected, including the Royal Free Hospital Subjective Global Assessment (RFH-SGA) as an indicator of protein-calorie malnutrition and the triceps skinfold thickness, which classified patients as having <em>normal</em> subcutaneous adipose tissue (SAT), <em>above average</em> SAT, and <em>below average</em> SAT. Follow-up was censored at the time of HCC diagnosis or LC decompensation.</div></div><div><h3>Results</h3><div>About 31/187 (16.0 %) and 22/187 (11.8 %) patients were categorized as having above- or below-average SAT at baseline, respectively. 10/187 patients (5.3 %) developed HCC during the compensated phase of LC at a median of 22 months (IQR: 10.0–36.75). A higher risk of HCC was observed in subjects below average SAT (HR: 4.064, CI 95 %: 1.012–16.317, <em>p</em> = 0.048). After adjusting the Cox models for age and α-fetoprotein at baseline, the statistical significance of the association between SAT and HCC was not modified.</div></div><div><h3>Conclusion</h3><div>These results suggest that decreased SAT may precede the diagnosis of HCC in compensated LC.</div></div>","PeriodicalId":8318,"journal":{"name":"Archives of Medical Research","volume":"56 3","pages":"Article 103127"},"PeriodicalIF":4.7,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142698345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-25DOI: 10.1016/j.arcmed.2024.103124
Elena Golovina , Juraj Kokavec , Dmitry Kazantsev , Oxana Yurikova , Martin Bajecny , Filipp Georgijevic Savvulidi , Radim Simersky , Rene Lenobel , Jorg Tost , Vit Herynek , Ludek Sefc , Marek Sebela , Pavel Klener , Zuzana Zemanova , Tomas Stopka , Karina Savvulidi Vargova
Background
Cell cycle progression and leukemia development are tightly regulated processes in which even a small imbalance in the expression of cell cycle regulatory molecules and microRNAs (miRNAs) can lead to an increased risk of cancer/leukemia development. Here, we focus on the study of a ubiquitous, multifunctional, and oncogenic miRNA-hsa-miR-155–5p (miR-155, MIR155HG), which is overexpressed in malignancies including chronic lymphocytic leukemia (CLL). Nonetheless, the precise mechanism of how miR-155 regulates the cell cycle in leukemic cells remains the subject of extensive research.
Methods
We edited the CLL cell line MEC-1 by CRISPR/Cas9 to introduce a short deletion within the MIR155HG gene. To describe changes at the transcriptome and miRNome level in miR-155-deficient cells, we performed mRNA-seq/miRNA-seq and validated changes by qRT-PCR. Flow cytometry was used to measure cell cycle kinetics. A WST-1 assay, hemocytometer, and Annexin V/PI staining assessed cell viability and proliferation.
Results
The limited but phenotypically robust miR-155 modification impaired cell proliferation, cell cycle, and cell ploidy. This was accompanied by overexpression of the negative cell cycle regulator p21/CDKN1A and Cyclin D1 (CCND1). We confirmed the overexpression of canonical miR-155 targets such as PU.1, FOS, SHIP-1, TP53INP1 and revealed new potential targets (FCRL5, ISG15, and MX1).
Conclusions
We demonstrate that miR-155 deficiency impairs cell proliferation, cell cycle, transcriptome, and miRNome via deregulation of the MIR155HG/TP53INP1/CDKN1A/CCND1 axis. Our CLL model is valuable for further studies to manipulate miRNA levels to revert highly aggressive leukemic cells to nearly benign or non-leukemic types.
{"title":"Deficiency of miR-155 in leukemic B-cells results in cell cycle arrest and deregulation of MIR155HG/TP53INP1/CDKN1A/CCND1 network","authors":"Elena Golovina , Juraj Kokavec , Dmitry Kazantsev , Oxana Yurikova , Martin Bajecny , Filipp Georgijevic Savvulidi , Radim Simersky , Rene Lenobel , Jorg Tost , Vit Herynek , Ludek Sefc , Marek Sebela , Pavel Klener , Zuzana Zemanova , Tomas Stopka , Karina Savvulidi Vargova","doi":"10.1016/j.arcmed.2024.103124","DOIUrl":"10.1016/j.arcmed.2024.103124","url":null,"abstract":"<div><h3>Background</h3><div>Cell cycle progression and leukemia development are tightly regulated processes in which even a small imbalance in the expression of cell cycle regulatory molecules and microRNAs (miRNAs) can lead to an increased risk of cancer/leukemia development. Here, we focus on the study of a ubiquitous, multifunctional, and oncogenic miRNA-hsa-miR-155–5p (miR-155, <em>MIR155HG</em>), which is overexpressed in malignancies including chronic lymphocytic leukemia (CLL). Nonetheless, the precise mechanism of how miR-155 regulates the cell cycle in leukemic cells remains the subject of extensive research.</div></div><div><h3>Methods</h3><div>We edited the CLL cell line MEC-1 by CRISPR/Cas9 to introduce a short deletion within the <em>MIR155HG</em> gene. To describe changes at the transcriptome and miRNome level in miR-155-deficient cells, we performed mRNA-seq/miRNA-seq and validated changes by qRT-PCR. Flow cytometry was used to measure cell cycle kinetics. A WST-1 assay, hemocytometer, and Annexin V/PI staining assessed cell viability and proliferation.</div></div><div><h3>Results</h3><div>The limited but phenotypically robust miR-155 modification impaired cell proliferation, cell cycle, and cell ploidy. This was accompanied by overexpression of the negative cell cycle regulator <em>p21/CDKN1A</em> and <em>Cyclin D1</em> (<em>CCND1</em>). We confirmed the overexpression of canonical miR-155 targets such as <em>PU.1, FOS, SHIP-1, TP53INP1</em> and revealed new potential targets (<em>FCRL5, ISG15,</em> and <em>MX1</em>).</div></div><div><h3>Conclusions</h3><div>We demonstrate that miR-155 deficiency impairs cell proliferation, cell cycle, transcriptome, and miRNome via deregulation of the <em>MIR155HG/TP53INP1/CDKN1A/CCND1</em> axis. Our CLL model is valuable for further studies to manipulate miRNA levels to revert highly aggressive leukemic cells to nearly benign or non-leukemic types.</div></div>","PeriodicalId":8318,"journal":{"name":"Archives of Medical Research","volume":"56 3","pages":"Article 103124"},"PeriodicalIF":4.7,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142698344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-22DOI: 10.1016/j.arcmed.2024.103113
Jesus Hernandez-Juarez , Victor Manuel Dominguez-Reyes , Jaime Garcia-Chavez , Manuel Moreno-Hernandez , Paola Itzel Carmona-Olvera , Jose Antonio Alvarado-Moreno , Guillermo Espejo-Godinez , Julieta Espinoza-Islas , Karim Majluf-Cruz , Rodrigo Arreola-Diaz , Patricia Cruz-Puente , Guadalupe Ortiz-Torres , Berenice Sanchez-Jara , Cecilia Rodriguez-Castillejos , Rosa Elena Sosa-Camas , Patricia Gomez-Rosas , Gregorio Campos-Cabrera , Abraham Majluf-Cruz
Background
Von Willebrand disease (VWD), is the most common inherited bleeding disorder worldwide, but its diagnosis is complicated, expensive, and poorly evaluated in several countries.
Objective
To report our long-term experience with the diagnosis of VWD based on a cost-effective strategy.
Methods
We studied 802 Mexican patients, men and women, children, and adults, with clinical suspicion of VWD. The following tests were performed: blood count, bleeding time, prothrombin time, activated partial thromboplastin time, fibrinogen concentration, VWF antigen, ristocetin cofactor activity, collagen binding assay, ristocetin-induced platelet aggregation, FVIII activity, and VWF multimers analysis.
Results
VWD was diagnosed in 639 patients; 582 had type 1 VWD (91.1%). Type 2 VWD was found in 52 patients (8.1%). Type 2A was present in 25 cases (48.1%), while types 2B and 2 M accounted for 21 (40.4) and six (11.5%) cases, respectively. Type 3 VWD was present in five patients (0.8%). The mean age of patients with VWD was 25.3 years (range: 2–71) for males and 22.1 (range: 1–54) for females. The diagnosis was inconclusive in 40 cases (5.0%) and was discarded in 123 (15.3%). Blood group O was the most common among patients with VWD.
Conclusion
Using a low-cost diagnostic strategy, we confirmed that VWD is as common in Mexico as in other countries. Review of the patient's history is mandatory when VWD is suspected, although laboratory confirmation may be difficult and expensive. The consequences of a lack of accurate and timely diagnosis affect the promptness and quality of treatment.
{"title":"A ten-year experience with the diagnosis of von Willebrand disease in Mexico based on a cost-effective strategy","authors":"Jesus Hernandez-Juarez , Victor Manuel Dominguez-Reyes , Jaime Garcia-Chavez , Manuel Moreno-Hernandez , Paola Itzel Carmona-Olvera , Jose Antonio Alvarado-Moreno , Guillermo Espejo-Godinez , Julieta Espinoza-Islas , Karim Majluf-Cruz , Rodrigo Arreola-Diaz , Patricia Cruz-Puente , Guadalupe Ortiz-Torres , Berenice Sanchez-Jara , Cecilia Rodriguez-Castillejos , Rosa Elena Sosa-Camas , Patricia Gomez-Rosas , Gregorio Campos-Cabrera , Abraham Majluf-Cruz","doi":"10.1016/j.arcmed.2024.103113","DOIUrl":"10.1016/j.arcmed.2024.103113","url":null,"abstract":"<div><h3>Background</h3><div>Von Willebrand disease (VWD), is the most common inherited bleeding disorder worldwide, but its diagnosis is complicated, expensive, and poorly evaluated in several countries.</div></div><div><h3>Objective</h3><div>To report our long-term experience with the diagnosis of VWD based on a cost-effective strategy.</div></div><div><h3>Methods</h3><div>We studied 802 Mexican patients, men and women, children, and adults, with clinical suspicion of VWD. The following tests were performed: blood count, bleeding time, prothrombin time, activated partial thromboplastin time, fibrinogen concentration, VWF antigen, ristocetin cofactor activity, collagen binding assay, ristocetin-induced platelet aggregation, FVIII activity, and VWF multimers analysis.</div></div><div><h3>Results</h3><div>VWD was diagnosed in 639 patients; 582 had type 1 VWD (91.1%). Type 2 VWD was found in 52 patients (8.1%). Type 2A was present in 25 cases (48.1%), while types 2B and 2 M accounted for 21 (40.4) and six (11.5%) cases, respectively. Type 3 VWD was present in five patients (0.8%). The mean age of patients with VWD was 25.3 years (range: 2–71) for males and 22.1 (range: 1–54) for females. The diagnosis was inconclusive in 40 cases (5.0%) and was discarded in 123 (15.3%). Blood group O was the most common among patients with VWD.</div></div><div><h3>Conclusion</h3><div>Using a low-cost diagnostic strategy, we confirmed that VWD is as common in Mexico as in other countries. Review of the patient's history is mandatory when VWD is suspected, although laboratory confirmation may be difficult and expensive. The consequences of a lack of accurate and timely diagnosis affect the promptness and quality of treatment.</div></div>","PeriodicalId":8318,"journal":{"name":"Archives of Medical Research","volume":"56 2","pages":"Article 103113"},"PeriodicalIF":4.7,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142696083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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