Pub Date : 2023-03-16DOI: 10.1080/03235408.2023.2192901
Abdul Razzaq, Arfan Ali, Sara Zahid, Arif Malik, L. Pengtao, Wànkuí Gǒng, Yuan Youlu, S. Ercişli, Muhammad Bilawal Junaid, M. Zafar
Abstract Cotton is white gold and contributes significantly to the economy of the countries. The process of evolution of resistance in various cotton insects directly affects the life of people. Among the cotton insect pests, pink bollworm (Pectinophora gossypiella) and army bollworm (Spodoptera litura) have turned out to be destructive. The development of seed using gene stacking approach has tremendously reduced the applications of agrochemicals making the technology greener for society. A gene cassette containing Cry11 and Cry1H genes was constructed. The cassette was cloned into the pCAMBIA2300 plant expression vector using the CAMV3-35S promoter. The construct was transformed into cotton line FBS-222 using the shoot apex-cut Agrobacterium-mediated transformation method. The expression of the Cry genes was 3-fold higher in transgenic than the non-transgenic cotton plants using qRT-PCR. Finally, the insect bioassay with transgenic cotton showed 90% mortality against pink bollworms and 80% against cotton army bollworms. The results demonstrate the development of seeds using the gene pyramiding approach is an effective strategy to control insect pest attacks.
{"title":"Engineering of cry genes “Cry11 and Cry1h” in cotton (Gossypium hirsutum L.) for protection against insect pest attack","authors":"Abdul Razzaq, Arfan Ali, Sara Zahid, Arif Malik, L. Pengtao, Wànkuí Gǒng, Yuan Youlu, S. Ercişli, Muhammad Bilawal Junaid, M. Zafar","doi":"10.1080/03235408.2023.2192901","DOIUrl":"https://doi.org/10.1080/03235408.2023.2192901","url":null,"abstract":"Abstract Cotton is white gold and contributes significantly to the economy of the countries. The process of evolution of resistance in various cotton insects directly affects the life of people. Among the cotton insect pests, pink bollworm (Pectinophora gossypiella) and army bollworm (Spodoptera litura) have turned out to be destructive. The development of seed using gene stacking approach has tremendously reduced the applications of agrochemicals making the technology greener for society. A gene cassette containing Cry11 and Cry1H genes was constructed. The cassette was cloned into the pCAMBIA2300 plant expression vector using the CAMV3-35S promoter. The construct was transformed into cotton line FBS-222 using the shoot apex-cut Agrobacterium-mediated transformation method. The expression of the Cry genes was 3-fold higher in transgenic than the non-transgenic cotton plants using qRT-PCR. Finally, the insect bioassay with transgenic cotton showed 90% mortality against pink bollworms and 80% against cotton army bollworms. The results demonstrate the development of seeds using the gene pyramiding approach is an effective strategy to control insect pest attacks.","PeriodicalId":8323,"journal":{"name":"Archives of Phytopathology and Plant Protection","volume":"56 1","pages":"384 - 396"},"PeriodicalIF":1.0,"publicationDate":"2023-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48576196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-16DOI: 10.1080/03235408.2023.2188993
E. Evallo, J. W. Taguiam, I. B. Posada, M. Balendres
Abstract Rambutan (Nephelium lappaceum) is an important fruit tree. Recently, leaf spots were observed in a tree in Los Baños, Laguna, Philippines. This study aims to identify the causal agent of rambutan leaf spot in the Philippines. Two fungal isolates, MBRAM001B and MBRAM003B, were isolated from the diseased leaves on potato dextrose agar medium. Colony growth and conidia characteristics of both isolates showed high resemblance with Colletotrichum species in the C. gloeosporioides species complex. Molecular characterisation of the isolates using the partial internal transcribed spacer rDNA and TUB2 regions identified the pathogens as C. tropicale MBRAM001B and C. siamense MBRAM003B. Leaf spots developed on rambutan leaves three days after inoculation and the same pathogens were isolated from the diseased leaves, establishing Koch’s Postulate. In conclusion, this study identified C. tropicale MBRAM001B and C. siamense MBRAM003B as causal agents of leaf spots in rambutan species in the Philippines. The survival and possible infection of these two Colletotrichum species on rambutan fruits and other fruit trees are worth further investigating.
{"title":"Two additional Colletotrichum species causing leaf spot of rambutan (Nephelium lappaceum)","authors":"E. Evallo, J. W. Taguiam, I. B. Posada, M. Balendres","doi":"10.1080/03235408.2023.2188993","DOIUrl":"https://doi.org/10.1080/03235408.2023.2188993","url":null,"abstract":"Abstract Rambutan (Nephelium lappaceum) is an important fruit tree. Recently, leaf spots were observed in a tree in Los Baños, Laguna, Philippines. This study aims to identify the causal agent of rambutan leaf spot in the Philippines. Two fungal isolates, MBRAM001B and MBRAM003B, were isolated from the diseased leaves on potato dextrose agar medium. Colony growth and conidia characteristics of both isolates showed high resemblance with Colletotrichum species in the C. gloeosporioides species complex. Molecular characterisation of the isolates using the partial internal transcribed spacer rDNA and TUB2 regions identified the pathogens as C. tropicale MBRAM001B and C. siamense MBRAM003B. Leaf spots developed on rambutan leaves three days after inoculation and the same pathogens were isolated from the diseased leaves, establishing Koch’s Postulate. In conclusion, this study identified C. tropicale MBRAM001B and C. siamense MBRAM003B as causal agents of leaf spots in rambutan species in the Philippines. The survival and possible infection of these two Colletotrichum species on rambutan fruits and other fruit trees are worth further investigating.","PeriodicalId":8323,"journal":{"name":"Archives of Phytopathology and Plant Protection","volume":"56 1","pages":"349 - 362"},"PeriodicalIF":1.0,"publicationDate":"2023-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44520478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-09DOI: 10.1080/03235408.2023.2185960
Halima Maina Abdullahi, Muhammed Lawan, Duna Madu Mailafiya
Abstract Field experiments were conducted at the Teaching and Research Farms of the University of Maiduguri and Mohamet Lawan College of Agriculture, Maiduguri, Nigeria to determine the effects of silica nanoparticles and tomato variety on the tomato fruit worm (Helicoverpa armigera Hübner) (Lepidoptera: Noctuidae). The treatments each replicated thrice were factorially combined and laid out in a split-plot design with four tomato varieties namely: UC-82, Dan Syria, Roma VF and Tima and three concentrations of amorphous silica nanoparticles (1000 ppm, 2000 ppm and 3000 ppm) and cypermethrin (as a check). Insecticides were applied during the fruiting stage at 7 days interval for 8 weeks. Larval holes and marketable fruit yield were recorded. Silica nanoparticles at 3000 ppm resulted in fewer larval population (39.25) at p < 0.05 with a resultant decrease in number of damaged fruits (36.58) as compared to lower doses of 1000 ppm and 2000 ppm. Cypermethrin also gave minimum population of larvae (39.12). In all tested varieties, Roma VF had the lowest larval population (28.79). Variety Roma VF in combination with both cypermethrin and silica nanoparticle at 3000 ppm resulted in lower larval population (19.67) and (28.67) respectively. These results show that silica nanoparticle at 3000 ppm acting together with Roma VF appreciably controls H. armigera in tomato, making both practices important components of IPM against this larval pest.
摘要在尼日利亚迈杜古里大学和穆罕默德·拉万农业学院的教学与研究农场进行了实地试验，以确定二氧化硅纳米颗粒和番茄品种对番茄果虫(Helicoverpa armigera h bner)(鳞翅目:夜蛾科)的影响。每个重复三次的处理进行因子组合，并以四种番茄品种(即:uc82, Dan Syria, Roma VF和Tima)和三种浓度的无定形二氧化硅纳米颗粒(1000 ppm, 2000 ppm和3000 ppm)和氯氰菊酯(作为对照)进行分块设计。结果期每隔7天施用杀虫剂，共施用8周。记录幼虫孔洞和商品果产量。与1000ppm和2000ppm较低剂量相比，3000ppm二氧化硅纳米颗粒导致幼虫数量减少(39.25)(p < 0.05)，因此受损果实数量减少(36.58)。氯氰菊酯对幼虫的影响最小(39.12)。在所有被试品种中，罗马螟幼虫数量最少(28.79)。用3000 ppm氯氰菊酯和二氧化硅纳米颗粒处理后，品种Roma VF幼虫数量分别为19.67和28.67。这些结果表明，纳米二氧化硅在3000 ppm浓度下与Roma VF共同作用，可显著控制番茄中的棉铃虫，使两者成为防治棉铃虫幼虫的重要组成部分。
{"title":"Silica nanoparticles and variety: their efficacy and interaction on the tomato fruit worm ( Helicoverpa armigera hubner) population infesting tomato (Solanum lycopersicum L.)","authors":"Halima Maina Abdullahi, Muhammed Lawan, Duna Madu Mailafiya","doi":"10.1080/03235408.2023.2185960","DOIUrl":"https://doi.org/10.1080/03235408.2023.2185960","url":null,"abstract":"Abstract Field experiments were conducted at the Teaching and Research Farms of the University of Maiduguri and Mohamet Lawan College of Agriculture, Maiduguri, Nigeria to determine the effects of silica nanoparticles and tomato variety on the tomato fruit worm (Helicoverpa armigera Hübner) (Lepidoptera: Noctuidae). The treatments each replicated thrice were factorially combined and laid out in a split-plot design with four tomato varieties namely: UC-82, Dan Syria, Roma VF and Tima and three concentrations of amorphous silica nanoparticles (1000 ppm, 2000 ppm and 3000 ppm) and cypermethrin (as a check). Insecticides were applied during the fruiting stage at 7 days interval for 8 weeks. Larval holes and marketable fruit yield were recorded. Silica nanoparticles at 3000 ppm resulted in fewer larval population (39.25) at p < 0.05 with a resultant decrease in number of damaged fruits (36.58) as compared to lower doses of 1000 ppm and 2000 ppm. Cypermethrin also gave minimum population of larvae (39.12). In all tested varieties, Roma VF had the lowest larval population (28.79). Variety Roma VF in combination with both cypermethrin and silica nanoparticle at 3000 ppm resulted in lower larval population (19.67) and (28.67) respectively. These results show that silica nanoparticle at 3000 ppm acting together with Roma VF appreciably controls H. armigera in tomato, making both practices important components of IPM against this larval pest.","PeriodicalId":8323,"journal":{"name":"Archives of Phytopathology and Plant Protection","volume":"56 1","pages":"335 - 348"},"PeriodicalIF":1.0,"publicationDate":"2023-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42839273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-25DOI: 10.1080/03235408.2023.2183796
C. Afolabi, S. Salihu, O. Shokalu
Abstract The production of Soybean in Nigeria is still inadequate due to the menace of pests and diseases. The current study was conducted to screen the performance of nineteen newly developed soybean lines that were infected naturally with bacterial diseases, identify promising line(s) with high yield potential and resistance against the diseases. Symptoms of soybean foliar diseases were accessed from the 4-12 week after planting, the resistance level of selected lines was also determined and bacteria isolate were culture and identify through morphological and biochemical tests. Four Bacteria species (Pseudomonas syringae, Xanthomonas spp, Clavibacter michiganensis and Bacillus subtilis) were isolated and identified from disease soybean leaf in this study. Whereas based on the pathogenicity test only two were pathogenic and cause soybean foliar disease (P. syringae and X. spp). Identified soybean lines differed significantly [p ≤ 0.05] in disease incidence and severity for both diseases. TGx 1990-57F, TGx 1990-55F were moderately resistant to bacterial blight and bacterial pustules. The study confirms that bacteria blight and pustule were present in the study area. It is therefore, recommended that the lines identified as moderately resistant to both diseases may help in future studies and evaluation in local hotspots.
{"title":"Screening of soybean [Glycine max (L.) merrill] lines for reaction to natural field infection and resistant against bacteria foliar diseases","authors":"C. Afolabi, S. Salihu, O. Shokalu","doi":"10.1080/03235408.2023.2183796","DOIUrl":"https://doi.org/10.1080/03235408.2023.2183796","url":null,"abstract":"Abstract The production of Soybean in Nigeria is still inadequate due to the menace of pests and diseases. The current study was conducted to screen the performance of nineteen newly developed soybean lines that were infected naturally with bacterial diseases, identify promising line(s) with high yield potential and resistance against the diseases. Symptoms of soybean foliar diseases were accessed from the 4-12 week after planting, the resistance level of selected lines was also determined and bacteria isolate were culture and identify through morphological and biochemical tests. Four Bacteria species (Pseudomonas syringae, Xanthomonas spp, Clavibacter michiganensis and Bacillus subtilis) were isolated and identified from disease soybean leaf in this study. Whereas based on the pathogenicity test only two were pathogenic and cause soybean foliar disease (P. syringae and X. spp). Identified soybean lines differed significantly [p ≤ 0.05] in disease incidence and severity for both diseases. TGx 1990-57F, TGx 1990-55F were moderately resistant to bacterial blight and bacterial pustules. The study confirms that bacteria blight and pustule were present in the study area. It is therefore, recommended that the lines identified as moderately resistant to both diseases may help in future studies and evaluation in local hotspots.","PeriodicalId":8323,"journal":{"name":"Archives of Phytopathology and Plant Protection","volume":"56 1","pages":"269 - 283"},"PeriodicalIF":1.0,"publicationDate":"2023-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41507088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-25DOI: 10.1080/03235408.2023.2183800
Janki N. Thakker, Jinal Badrakia, Keyur B. Patel, Ujawala Makwana, Kinjal J. Parmar, P. Dhandhukia
Abstract Present study focuses on the use of bacterial isolate Pseudomonas OG101 against the Fusarium sp. and its growth promoting ability for chickpea (Cicer arietinum) and cowpea (Vigna unguiculata) plants. Pseudomonas strain OG101 controls mycelial growth of Fusarium oxysporum f.sp. ciceris and F. oxysporum f.sp. pallidoroseum up to 24.4% and 20.5%, respectively. In addition, OG101 showed a significant improvement in the germination index of 93.3% and 98.3% with disease index of 1.6% and 3.3% in chickpea plant and cowpea plant, respectively. Pot experiments of chickpea and cowpea plants refer to use it as a seed bacterization for the best effect for growth promotion and its biocontrol activity against F. oxysporum f.sp. ciceris and F. oxysporum f.sp. pallidoroseum respectively.
{"title":"Potential of a marine Pseudomonas aeruginosa strain OG101 to combat Fusarium oxysporum associated wilt in legume crops","authors":"Janki N. Thakker, Jinal Badrakia, Keyur B. Patel, Ujawala Makwana, Kinjal J. Parmar, P. Dhandhukia","doi":"10.1080/03235408.2023.2183800","DOIUrl":"https://doi.org/10.1080/03235408.2023.2183800","url":null,"abstract":"Abstract Present study focuses on the use of bacterial isolate Pseudomonas OG101 against the Fusarium sp. and its growth promoting ability for chickpea (Cicer arietinum) and cowpea (Vigna unguiculata) plants. Pseudomonas strain OG101 controls mycelial growth of Fusarium oxysporum f.sp. ciceris and F. oxysporum f.sp. pallidoroseum up to 24.4% and 20.5%, respectively. In addition, OG101 showed a significant improvement in the germination index of 93.3% and 98.3% with disease index of 1.6% and 3.3% in chickpea plant and cowpea plant, respectively. Pot experiments of chickpea and cowpea plants refer to use it as a seed bacterization for the best effect for growth promotion and its biocontrol activity against F. oxysporum f.sp. ciceris and F. oxysporum f.sp. pallidoroseum respectively.","PeriodicalId":8323,"journal":{"name":"Archives of Phytopathology and Plant Protection","volume":"56 1","pages":"284 - 294"},"PeriodicalIF":1.0,"publicationDate":"2023-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44275299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-25DOI: 10.1080/03235408.2023.2184232
M. Ali, S. Afzaal, F. Sharif, S. W. Ali, H. Nawaz, W. Khan
Abstract The genus Pythium belongs to the soilborne Oomycetes. This research was conducted to characterize Pythium species from Punjab, Pakistan’s agricultural lands. Total of eleven Pythium (P. debaryanum, P. aphanidermatum, P. spinosum, P. irregulare, P. myriotylum, P. acanthicum, P. vexans, P. graminicola, P. dissotocum, P. echinulatum, and P. insidiosum) were isolated from the samples. Biometrics revealed the maximum values for aplerotic index were 59.8% for P. acanthicum and P. dissotocum while maximum values for ooplast index (39.6%) were calculated in the case of P. myriotylum and P. vexans. Molecular characterization, amplification of the ITS region revealed a genome size of approximately 570 bp. NCBI accession numbers for each species were acquired, and each species has shown 98–99% similarity with their reference sequences in the gene bank database. The research is a much-needed step to identify these pathogenic fungi morphologically and molecularly to understand its epidemiology and develop some potential management strategies.
{"title":"Morphological and molecular characterization of Pythium species from Punjab, Pakistan","authors":"M. Ali, S. Afzaal, F. Sharif, S. W. Ali, H. Nawaz, W. Khan","doi":"10.1080/03235408.2023.2184232","DOIUrl":"https://doi.org/10.1080/03235408.2023.2184232","url":null,"abstract":"Abstract The genus Pythium belongs to the soilborne Oomycetes. This research was conducted to characterize Pythium species from Punjab, Pakistan’s agricultural lands. Total of eleven Pythium (P. debaryanum, P. aphanidermatum, P. spinosum, P. irregulare, P. myriotylum, P. acanthicum, P. vexans, P. graminicola, P. dissotocum, P. echinulatum, and P. insidiosum) were isolated from the samples. Biometrics revealed the maximum values for aplerotic index were 59.8% for P. acanthicum and P. dissotocum while maximum values for ooplast index (39.6%) were calculated in the case of P. myriotylum and P. vexans. Molecular characterization, amplification of the ITS region revealed a genome size of approximately 570 bp. NCBI accession numbers for each species were acquired, and each species has shown 98–99% similarity with their reference sequences in the gene bank database. The research is a much-needed step to identify these pathogenic fungi morphologically and molecularly to understand its epidemiology and develop some potential management strategies.","PeriodicalId":8323,"journal":{"name":"Archives of Phytopathology and Plant Protection","volume":"56 1","pages":"307 - 321"},"PeriodicalIF":1.0,"publicationDate":"2023-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47579409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-25DOI: 10.1080/03235408.2023.2184231
M. Osman, A. El-Nuby
Abstract A new pathogenic fungus was noticed in 2020 for the first time as white rot on cuttings of sugarcane-grown fields of El-Mattana district (Luxor governorate), Kom-Ombo district (Asswan governorate) and Qus district (Qena governorate), Egypt. The causal organism was isolated from affected cuttings using Koch’s postulates, during 2021 and repeated at 2022, the relationship between the recovered isolate and the observed symptoms had been established. Artificially inoculated cuttings of sugarcane cultivar G.T. 54-9 gave symptoms similar to those appeared on naturally field-infected sugarcane cuttings. Morphological characters and molecular analysis, based on fungal internal transcribed spacer (ITS), were carried out to detect the recovered isolate. Identification of fungal pathogen revealed that Efibula tuberculata, Basidiomycota, was the causal organism of the white-rot of cuttings and roots of sugarcane. According to the available literature, this was the first report of occurring E. tuberculata isolate lux in Egypt and also as a sugarcane pathogen globally. Further studies to find the potent strategy to control, determine the susceptibility of different sugarcane cultivars and the fungus host range are needed.
{"title":"First record of Efibula tuberculata (Basidiomycota: Polyporales) isolated from sugarcane in Egypt","authors":"M. Osman, A. El-Nuby","doi":"10.1080/03235408.2023.2184231","DOIUrl":"https://doi.org/10.1080/03235408.2023.2184231","url":null,"abstract":"Abstract A new pathogenic fungus was noticed in 2020 for the first time as white rot on cuttings of sugarcane-grown fields of El-Mattana district (Luxor governorate), Kom-Ombo district (Asswan governorate) and Qus district (Qena governorate), Egypt. The causal organism was isolated from affected cuttings using Koch’s postulates, during 2021 and repeated at 2022, the relationship between the recovered isolate and the observed symptoms had been established. Artificially inoculated cuttings of sugarcane cultivar G.T. 54-9 gave symptoms similar to those appeared on naturally field-infected sugarcane cuttings. Morphological characters and molecular analysis, based on fungal internal transcribed spacer (ITS), were carried out to detect the recovered isolate. Identification of fungal pathogen revealed that Efibula tuberculata, Basidiomycota, was the causal organism of the white-rot of cuttings and roots of sugarcane. According to the available literature, this was the first report of occurring E. tuberculata isolate lux in Egypt and also as a sugarcane pathogen globally. Further studies to find the potent strategy to control, determine the susceptibility of different sugarcane cultivars and the fungus host range are needed.","PeriodicalId":8323,"journal":{"name":"Archives of Phytopathology and Plant Protection","volume":"56 1","pages":"295 - 306"},"PeriodicalIF":1.0,"publicationDate":"2023-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45027187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-25DOI: 10.1080/03235408.2023.2184233
M. Öztürk, H. Aksoy, Şahane Funda Arslanoglu
Abstract Pectobacterium species are one of the main bacterial agents of soft rot and blackleg diseases that cause economic damage to potato crops. Farmers around the world suffer losses from both diseases and use different strategies to control. In this study, local potato genotypes were evaluated for disease severity of soft rot and blackleg. We discovered putative genotypes with low disease severity for both diseases. In the tuber experiment, the lowest soft rot was determined for genotype 18 with 34% disease severity. The highest soft rot was found in the cultivars Natasha, Laperla and Sultanecem. In the plant experiment, the lowest lesion length was measured in the cultivars Sultannur, Orchestra and Marabel. The 33% disease severity for these cultivars was also calculated for genotypes 18 and 32. With regard to soft rot and blackleg of potato, this is the first study to determine disease severity of Turkish local potato genotypes.
{"title":"Determination of the severity of soft rot and blackleg caused by Pectobacterium brasiliense in some potato genotypes from Turkey","authors":"M. Öztürk, H. Aksoy, Şahane Funda Arslanoglu","doi":"10.1080/03235408.2023.2184233","DOIUrl":"https://doi.org/10.1080/03235408.2023.2184233","url":null,"abstract":"Abstract Pectobacterium species are one of the main bacterial agents of soft rot and blackleg diseases that cause economic damage to potato crops. Farmers around the world suffer losses from both diseases and use different strategies to control. In this study, local potato genotypes were evaluated for disease severity of soft rot and blackleg. We discovered putative genotypes with low disease severity for both diseases. In the tuber experiment, the lowest soft rot was determined for genotype 18 with 34% disease severity. The highest soft rot was found in the cultivars Natasha, Laperla and Sultanecem. In the plant experiment, the lowest lesion length was measured in the cultivars Sultannur, Orchestra and Marabel. The 33% disease severity for these cultivars was also calculated for genotypes 18 and 32. With regard to soft rot and blackleg of potato, this is the first study to determine disease severity of Turkish local potato genotypes.","PeriodicalId":8323,"journal":{"name":"Archives of Phytopathology and Plant Protection","volume":"56 1","pages":"322 - 333"},"PeriodicalIF":1.0,"publicationDate":"2023-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43664077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-07DOI: 10.1080/03235408.2023.2183792
Gulbir Singh, Kuldeep Kumar Yogi
Abstract Plant diseases have a serious impact on production, and hence they must be detected and recognised at early stages. Smart firming using deep learning can automatically identify infected crops. We provide extremely effective convolution neural network (CNN) designs for the identification of leaf diseases in this research strategy. For the training and testing phases of this study, a database of potato leaves is produced. To classify the disease from the input photos of the supported training dataset, we employed CNN to extract its characteristics. 1700 photos of potato leaves were used for model training, and then about 600 images were used for testing. To identify citrus diseases, Convolutional Neural Networks, Deep Learning, base learning, and transfer learning were applied. Results from training, testing, and experiments indicate that the suggested architecture has outperformed other current models in terms of ResNet model accuracy, achieving a score of 99.62%.
{"title":"Performance evaluation of plant leaf disease detection using deep learning models","authors":"Gulbir Singh, Kuldeep Kumar Yogi","doi":"10.1080/03235408.2023.2183792","DOIUrl":"https://doi.org/10.1080/03235408.2023.2183792","url":null,"abstract":"Abstract Plant diseases have a serious impact on production, and hence they must be detected and recognised at early stages. Smart firming using deep learning can automatically identify infected crops. We provide extremely effective convolution neural network (CNN) designs for the identification of leaf diseases in this research strategy. For the training and testing phases of this study, a database of potato leaves is produced. To classify the disease from the input photos of the supported training dataset, we employed CNN to extract its characteristics. 1700 photos of potato leaves were used for model training, and then about 600 images were used for testing. To identify citrus diseases, Convolutional Neural Networks, Deep Learning, base learning, and transfer learning were applied. Results from training, testing, and experiments indicate that the suggested architecture has outperformed other current models in terms of ResNet model accuracy, achieving a score of 99.62%.","PeriodicalId":8323,"journal":{"name":"Archives of Phytopathology and Plant Protection","volume":"56 1","pages":"209 - 233"},"PeriodicalIF":1.0,"publicationDate":"2023-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47817556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-07DOI: 10.1080/03235408.2023.2184230
H. C. Nguyen, Thi Tho Nguyen, Thi Nhung Lam, Thi Trang Le, Phuong Lan Le, Tien Hung Nguyen, Xuan Hoan Dinh
Abstract The rice bacterial blight caused by Xanthomonas oryzae pv. oryzae (Xoo) bacteria is rice’s most destructive disease. Due to the potential biomagnification caused by bactericides, deploying host resistance is the most effective method to protect the rice harvest. Studying the pathogenicity structure of the Xoo population helps propose the best breeding strategies to use the resistance gene pool. Among 12 resistance genes validated, xa5 and Xa21 maintained their efficacy against the Xoo population in Vietnam. Other genes, including Xa7 and xa13, remained effective in certain regions. The most destructive Xoo races distribute in the South, where intensive rice production was practiced. The same Xoo races found in different ecological regions illustrated the pathogen migration between adjacent areas. This manuscript proposed a designation system that converts the response of the differential set to each Xoo isolate into a binary, then into an octal system to name Xoo races appropriately.
{"title":"Pathogenicity structure of the Xanthomonas oryzae pv. oryzae population causing rice bacterial blight in Vietnam","authors":"H. C. Nguyen, Thi Tho Nguyen, Thi Nhung Lam, Thi Trang Le, Phuong Lan Le, Tien Hung Nguyen, Xuan Hoan Dinh","doi":"10.1080/03235408.2023.2184230","DOIUrl":"https://doi.org/10.1080/03235408.2023.2184230","url":null,"abstract":"Abstract The rice bacterial blight caused by Xanthomonas oryzae pv. oryzae (Xoo) bacteria is rice’s most destructive disease. Due to the potential biomagnification caused by bactericides, deploying host resistance is the most effective method to protect the rice harvest. Studying the pathogenicity structure of the Xoo population helps propose the best breeding strategies to use the resistance gene pool. Among 12 resistance genes validated, xa5 and Xa21 maintained their efficacy against the Xoo population in Vietnam. Other genes, including Xa7 and xa13, remained effective in certain regions. The most destructive Xoo races distribute in the South, where intensive rice production was practiced. The same Xoo races found in different ecological regions illustrated the pathogen migration between adjacent areas. This manuscript proposed a designation system that converts the response of the differential set to each Xoo isolate into a binary, then into an octal system to name Xoo races appropriately.","PeriodicalId":8323,"journal":{"name":"Archives of Phytopathology and Plant Protection","volume":"56 1","pages":"256 - 268"},"PeriodicalIF":1.0,"publicationDate":"2023-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47654175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：