Pub Date : 2022-01-01DOI: 10.21687/0233-528x-2022-56-4-89-94
A. Lishnevskii, O. A. Ivanova, K. O. Inozemtsev, A. Hirn, I. Apáthy, B. Zábori, A. Csőke, S. Deme, T. Pázmándi, P. Szántó, D. Kartashov, R. Tolochek, I. Kartsev, V. Shurshakov
The paper reports the results of measuring absorbed doses and quality factor of ionizing space radiation in the ISS Service module in June-October of 2020 with the use of dosimeter Tritel as part of space experiment Matreshka-R. Mean dose rate over the period amounted to 0.38 ± 0.03 mGy/d. Experimental data on the dose rate dynamics allow to set aside contributions of Earth's radiation belts and galactic cosmic radiation in the total daily dose. The daily average quality factors made up 3.05 ± 0.16 for total dose, 1.6 ± 0.1 for Earth's radiation belts and 5.1 ± 0.3 for galactic space radiation.
{"title":"MONITORING RADIATION LOADS AND QUALITY FACTOR OF IONIZING SPACE RADIATION IN THE ISS SERVICE MODULE WITH THE USE OF RESEARCH EQUIPMENT \"TRITEL\"","authors":"A. Lishnevskii, O. A. Ivanova, K. O. Inozemtsev, A. Hirn, I. Apáthy, B. Zábori, A. Csőke, S. Deme, T. Pázmándi, P. Szántó, D. Kartashov, R. Tolochek, I. Kartsev, V. Shurshakov","doi":"10.21687/0233-528x-2022-56-4-89-94","DOIUrl":"https://doi.org/10.21687/0233-528x-2022-56-4-89-94","url":null,"abstract":"The paper reports the results of measuring absorbed doses and quality factor of ionizing space radiation in the ISS Service module in June-October of 2020 with the use of dosimeter Tritel as part of space experiment Matreshka-R. Mean dose rate over the period amounted to 0.38 ± 0.03 mGy/d. Experimental data on the dose rate dynamics allow to set aside contributions of Earth's radiation belts and galactic cosmic radiation in the total daily dose. The daily average quality factors made up 3.05 ± 0.16 for total dose, 1.6 ± 0.1 for Earth's radiation belts and 5.1 ± 0.3 for galactic space radiation.","PeriodicalId":8683,"journal":{"name":"Aviakosmicheskaia i ekologicheskaia meditsina = Aerospace and environmental medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67955539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.21687/0233-528x-2022-56-5-65-76
S. Belova, К.А. Zaripova, К.А. Sharlo, B. Shenkman, T. Nemirovskaya
We tested the hypothesis that in unloaded skeletal muscles the calcium-dependent signaling pathways and E3-ligase expression are controlled by regulation of phosphorylation of AMP-activated protein kinase (AMPK). For a 3-d experiment 32 Wister male rats were divided into 4 groups: placebo control (С), metformin control (300 mg/kg of body, per oral, MC), suspension and placebo (SP), suspension and metformin (SM). Object of the investigation was m. soleus. In comparison to group C, rAMPK in group MC reduced 46 % and ATP increased 49 % (p < 0.05); pCaMK II showed an increase and expression of mRNA CaN, SERCA2a and Calpain 1 grew 483 %, 87 %, 41 % and 62 %, respectively; p < 0.05). In group SP, MuRF1, MAFbx E3-ligase expression and ubiquitin increased 167 %, 146 % and 191 %, respectively (p < 0.05). Per oral metformin prevented these changes in the suspended rats. During 3 days of suspension, metformin prevented changes in rAMK and ATP; also, it influenced regulation of the calcium-dependent signaling pathways through expression and phosphorylation of such markers as CaMK, CaN, SERCA2a and Calpain-1, and prevented to a degree growth in expression of key markers of ubuquitin-proteasome pathway – MuRF1, MAFbx, and uniquitin.
{"title":"ROLE OF AMP-ACTIVATED PROTEIN KINASE IN REGULATION OF CALCIUM-DEPENDENT AND UBIQUITIN/PROTEASOME SIGNALING PATHWAYS DURING MUSCLE FUNCTIONAL DISLOADING","authors":"S. Belova, К.А. Zaripova, К.А. Sharlo, B. Shenkman, T. Nemirovskaya","doi":"10.21687/0233-528x-2022-56-5-65-76","DOIUrl":"https://doi.org/10.21687/0233-528x-2022-56-5-65-76","url":null,"abstract":"We tested the hypothesis that in unloaded skeletal muscles the calcium-dependent signaling pathways and E3-ligase expression are controlled by regulation of phosphorylation of AMP-activated protein kinase (AMPK). For a 3-d experiment 32 Wister male rats were divided into 4 groups: placebo control (С), metformin control (300 mg/kg of body, per oral, MC), suspension and placebo (SP), suspension and metformin (SM). Object of the investigation was m. soleus. In comparison to group C, rAMPK in group MC reduced 46 % and ATP increased 49 % (p < 0.05); pCaMK II showed an increase and expression of mRNA CaN, SERCA2a and Calpain 1 grew 483 %, 87 %, 41 % and 62 %, respectively; p < 0.05). In group SP, MuRF1, MAFbx E3-ligase expression and ubiquitin increased 167 %, 146 % and 191 %, respectively (p < 0.05). Per oral metformin prevented these changes in the suspended rats. During 3 days of suspension, metformin prevented changes in rAMK and ATP; also, it influenced regulation of the calcium-dependent signaling pathways through expression and phosphorylation of such markers as CaMK, CaN, SERCA2a and Calpain-1, and prevented to a degree growth in expression of key markers of ubuquitin-proteasome pathway – MuRF1, MAFbx, and uniquitin.","PeriodicalId":8683,"journal":{"name":"Aviakosmicheskaia i ekologicheskaia meditsina = Aerospace and environmental medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67956401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.21687/0233-528x-2022-56-6-31-36
L.Kh. Pastuhkova, A. Goncharova, D. N. Kashirina, A.N. Chebotok, A. Kononikhin, A. G. Brzhozovsky, I. Larina, Е.А. Ilyin, O. I. Orlov
Chromatography-mass spectrometry of dried blood spots sampled during the year-long wintering of the 65th Russian expedition to the Antarctic station Vostok identified 6798 peptides. The maximal confidence level made possible the classification of 1239 proteins. Named were proteins that changed concentrations by months 4 and 12 of wintering. Crude proteins typical for these periods were identified, too. Methods of bioinformatics established period-specific as well as common changes in proteins on the level of confidentiality. The proteomic data allow discussion of signaling pathways and ways the organism adapts to extreme life environments. Our results suggest that professional activities in the conditions of long-term hypobaric hypoxia, isolation and hypokinesia on Antarctic station «Vostok» are accompanied by changes in the blood proteome affecting first and foremost the processes that control chemical and energy homeostasis on the molecular, cell, tissue, organ and system levels.
{"title":"CHARACTERIZATION OF A HEALTHY HUMAN BLOOD COMPOSITION IN THE COURSE OF A YEAR-LONG STAY ON THE ANTARCTIC STATION «VOSTOK»","authors":"L.Kh. Pastuhkova, A. Goncharova, D. N. Kashirina, A.N. Chebotok, A. Kononikhin, A. G. Brzhozovsky, I. Larina, Е.А. Ilyin, O. I. Orlov","doi":"10.21687/0233-528x-2022-56-6-31-36","DOIUrl":"https://doi.org/10.21687/0233-528x-2022-56-6-31-36","url":null,"abstract":"Chromatography-mass spectrometry of dried blood spots sampled during the year-long wintering of the 65th Russian expedition to the Antarctic station Vostok identified 6798 peptides. The maximal confidence level made possible the classification of 1239 proteins. Named were proteins that changed concentrations by months 4 and 12 of wintering. Crude proteins typical for these periods were identified, too. Methods of bioinformatics established period-specific as well as common changes in proteins on the level of confidentiality. The proteomic data allow discussion of signaling pathways and ways the organism adapts to extreme life environments. Our results suggest that professional activities in the conditions of long-term hypobaric hypoxia, isolation and hypokinesia on Antarctic station «Vostok» are accompanied by changes in the blood proteome affecting first and foremost the processes that control chemical and energy homeostasis on the molecular, cell, tissue, organ and system levels.","PeriodicalId":8683,"journal":{"name":"Aviakosmicheskaia i ekologicheskaia meditsina = Aerospace and environmental medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67956582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.21687/0233-528x-2022-56-6-5-15
T. V. Samoilenko, V. V. Shishkina, L. N. Antakova, D. A. Atyakshin
This is an overview of the current knowledge of the structural and functional organization of the smooth muscle tissue (SMT) presenting equally fundamental and applied interest for space biomedicine. SMT is a structural component of the visceral organs involved in adaptive reactions or pathological developments. According to the evidence, cell populations of visceral SMT contain smooth myocites differing in histophysiological properties. Many investigators reason that the SMT morphological and embriological origin is organ-specific and can be an area of translational researches, including space biomedicine. In spite of the multisided analysis of animal and humans tissues exposed to the spaceflight factors, no effort has been made to study SMT in space microgravity or ground-based modeling experiments. However, space gastroenterology knows facts about morphological transformations in the digestive organs in the spaceflight environment. Clearly, SMT as a structural component of the gastrointestinal tract may contribute directly in a number of organ-specific changes during orbital missions. SMT studies will unveil mechanisms of adaptive cell, and molecular changes with stromal mediation due to microgravity, and reveal targets for enhancing health control in space missions.
{"title":"SMOOTH MUSCLE TISSUE – A POTENTIAL TARGET OF TRANSLATIONAL RESEARCHES IN SPACE BIOMEDICINE","authors":"T. V. Samoilenko, V. V. Shishkina, L. N. Antakova, D. A. Atyakshin","doi":"10.21687/0233-528x-2022-56-6-5-15","DOIUrl":"https://doi.org/10.21687/0233-528x-2022-56-6-5-15","url":null,"abstract":"This is an overview of the current knowledge of the structural and functional organization of the smooth muscle tissue (SMT) presenting equally fundamental and applied interest for space biomedicine. SMT is a structural component of the visceral organs involved in adaptive reactions or pathological developments. According to the evidence, cell populations of visceral SMT contain smooth myocites differing in histophysiological properties. Many investigators reason that the SMT morphological and embriological origin is organ-specific and can be an area of translational researches, including space biomedicine. In spite of the multisided analysis of animal and humans tissues exposed to the spaceflight factors, no effort has been made to study SMT in space microgravity or ground-based modeling experiments. However, space gastroenterology knows facts about morphological transformations in the digestive organs in the spaceflight environment. Clearly, SMT as a structural component of the gastrointestinal tract may contribute directly in a number of organ-specific changes during orbital missions. SMT studies will unveil mechanisms of adaptive cell, and molecular changes with stromal mediation due to microgravity, and reveal targets for enhancing health control in space missions.","PeriodicalId":8683,"journal":{"name":"Aviakosmicheskaia i ekologicheskaia meditsina = Aerospace and environmental medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67957160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.21687/0233-528x-2022-56-6-60-65
Yu. B. Moiseev, I. Kovalchuk, M. Rybnikova, Yu.I. Remizov
Investigation with participation of the first- to fourth-year female students of the flight school in Krasnodar had the purpose to ascertain that sizes of the present pilot gear fit modern types of the female body. It was found out that sizes of many female students are below minimal ranges of the pilot gear fit. The quantity of these female students varies from 11 % (anti-g suits) to 100 % (O2 masks). The group of female students was actually representative of the women of the same age in this country. We conclude that the existing misses' sizes do not fit the anthropometric parameters of female candidates for flight training. To sum up, there is need to revise the size ranges of the flight gear with consideration of modern types of the female body.
{"title":"ANTHROPOMETRIC PROFILE OF FEMALE STUDENTS OF A FLIGHT SCHOOL","authors":"Yu. B. Moiseev, I. Kovalchuk, M. Rybnikova, Yu.I. Remizov","doi":"10.21687/0233-528x-2022-56-6-60-65","DOIUrl":"https://doi.org/10.21687/0233-528x-2022-56-6-60-65","url":null,"abstract":"Investigation with participation of the first- to fourth-year female students of the flight school in Krasnodar had the purpose to ascertain that sizes of the present pilot gear fit modern types of the female body. It was found out that sizes of many female students are below minimal ranges of the pilot gear fit. The quantity of these female students varies from 11 % (anti-g suits) to 100 % (O2 masks). The group of female students was actually representative of the women of the same age in this country. We conclude that the existing misses' sizes do not fit the anthropometric parameters of female candidates for flight training. To sum up, there is need to revise the size ranges of the flight gear with consideration of modern types of the female body.","PeriodicalId":8683,"journal":{"name":"Aviakosmicheskaia i ekologicheskaia meditsina = Aerospace and environmental medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67957352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.21687/0233-528x-2022-56-6-79-87
A.M. Belyak, А.А. Shilovich, A. S. Krivobok, V. B. Nikitin, T. Bibikova
The paper presents the results of experiments with 48-hour horizontal and vertical clinostatting of 5-day old Arabidopsis thaliana seedlings in 2 types of lighting, i.e. white light (4000 cd) and lateral blue light (450 nm). The experiments were performed in a clinostat designed to install Petri dishes with juvenile Arabidopsis thaliana in the horizontal and rotate it about the horizontal axis at a speed of 1 rev/min. Angular bend of the main root (MR) was measured as it elongated. It was demonstrated that, on the average, vertical clinostating in white light caused a more significant MR departure from the apical-basis axis than the horizontal clinostating. The proposed space experiments with Arabidopsis seedlings and laboratory controls will require extensive preliminary studies of the effects of specific light spectra and directions, and crop orientation. In blue light, there was no difference in the MR departure due to the seedlings placement in the clinostat; however, more than 75 % of the seedlings tended to bend away from the light source. Directed blue lighting in a 2D-clinostat can be an approach to root system orientation based on the negative phototropism.
{"title":"DETERMINATION OF THE EFFECT OF DIRECTED LIGHTING BY BLUE LIGHT-EMITTING DIODES ON GROWTH OF TAP ROOTS OF ARABIDOPSIS THALIANA SEEDLINGS DURING SLOW 2D-CLINOSTAT ROTATION","authors":"A.M. Belyak, А.А. Shilovich, A. S. Krivobok, V. B. Nikitin, T. Bibikova","doi":"10.21687/0233-528x-2022-56-6-79-87","DOIUrl":"https://doi.org/10.21687/0233-528x-2022-56-6-79-87","url":null,"abstract":"The paper presents the results of experiments with 48-hour horizontal and vertical clinostatting of 5-day old Arabidopsis thaliana seedlings in 2 types of lighting, i.e. white light (4000 cd) and lateral blue light (450 nm). The experiments were performed in a clinostat designed to install Petri dishes with juvenile Arabidopsis thaliana in the horizontal and rotate it about the horizontal axis at a speed of 1 rev/min. Angular bend of the main root (MR) was measured as it elongated. It was demonstrated that, on the average, vertical clinostating in white light caused a more significant MR departure from the apical-basis axis than the horizontal clinostating. The proposed space experiments with Arabidopsis seedlings and laboratory controls will require extensive preliminary studies of the effects of specific light spectra and directions, and crop orientation. In blue light, there was no difference in the MR departure due to the seedlings placement in the clinostat; however, more than 75 % of the seedlings tended to bend away from the light source. Directed blue lighting in a 2D-clinostat can be an approach to root system orientation based on the negative phototropism.","PeriodicalId":8683,"journal":{"name":"Aviakosmicheskaia i ekologicheskaia meditsina = Aerospace and environmental medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67957558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.21687/0233-528x-2022-56-1-26-31
E. A. Kapustina, E. Titov, М.А. Novikov
No more than 100 nm large, nanoparticles (NPs) are distinguished by unusual mechanical, electrical, optical, thermal and magnetic properties. They are used in biology, pharmacology, medicine, chemistry, physics, materials technology and engineering industry. Nanoparticles penetrate in organism through the skin, GIT and airways. Their genotoxicity depends on size, form, mode of action and composition. Two principal mechanisms of NPs genotoxicity are primary and secondary. The primary mechanism is realized in direct interaction with the genome; the secondary mechanism is achieved through mediation of the active forms of oxygen. NPs can modulate the epigenome by altering the gene functions and do not change the DNA sequence directly. Consequences of prolonged exposure to low NPs doses on the human organism still remain uninvestigated.
{"title":"GENOTOXICITY OF METAL-CONTAINING NANOPARTICLES","authors":"E. A. Kapustina, E. Titov, М.А. Novikov","doi":"10.21687/0233-528x-2022-56-1-26-31","DOIUrl":"https://doi.org/10.21687/0233-528x-2022-56-1-26-31","url":null,"abstract":"No more than 100 nm large, nanoparticles (NPs) are distinguished by unusual mechanical, electrical, optical, thermal and magnetic properties. They are used in biology, pharmacology, medicine, chemistry, physics, materials technology and engineering industry. Nanoparticles penetrate in organism through the skin, GIT and airways. Their genotoxicity depends on size, form, mode of action and composition. Two principal mechanisms of NPs genotoxicity are primary and secondary. The primary mechanism is realized in direct interaction with the genome; the secondary mechanism is achieved through mediation of the active forms of oxygen. NPs can modulate the epigenome by altering the gene functions and do not change the DNA sequence directly. Consequences of prolonged exposure to low NPs doses on the human organism still remain uninvestigated.","PeriodicalId":8683,"journal":{"name":"Aviakosmicheskaia i ekologicheskaia meditsina = Aerospace and environmental medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67949879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.21687/0233-528x-2022-56-2-11-19
D. D. Vlasova, A. Sadova, V. Galina, N.S. Guermanov, M. Rykova, E. Antropova, O. Kutko, S. Shulguina, K. Orlova, V. A. Shmarov, E. A. Lysenko, S. Ponomarev
Expression of inborn immunity genes was studied in 10 essentially healthy male subjects aged 24 to 32 years in a 21-day dry immersion experiment without countermeasures. To evaluate the humoral immunity function, genes encoding Toll-like receptors, proteins mediating NF-kB, AP-1, Akt, TLR4; apoptosis proteins, and CLEC4E, HSPD1, PTGS2 were selected. Fenol-cloroform extraction was used to obtain total RNA from peripheral blood monocytes CD14+. Subsequently, total RNA was reverse transcribed to generate cDNA for real-time PCR. The results showed substantial changes in expression of these genes suggesting that changes in the TLRs function on the transcriptional level were at the very start of the immune response to 21-d DI.
本研究对10名24 ~ 32岁基本健康男性受试者进行了为期21天的无防制浸泡实验，研究了先天免疫基因的表达。为了评估体液免疫功能，研究了toll样受体编码基因、NF-kB、AP-1、Akt、TLR4介导蛋白;细胞凋亡蛋白，以及cle4e、HSPD1、PTGS2。采用苯酚-氯仿萃取法提取外周血单核细胞CD14+总RNA。随后，逆转录总RNA生成cDNA进行实时PCR。结果显示，这些基因的表达发生了实质性的变化，这表明TLRs功能在转录水平上的变化发生在对21 d DI的免疫反应的最初阶段。
{"title":"EFFECT OF 21-DAY DRY IMMESRION ON EXPRESSION OF INBORN IMMUNITY GENES ASSOCIATED WITH THE TOLL-LIKE RECEPTORS' SIGNALIING PATHWAYS","authors":"D. D. Vlasova, A. Sadova, V. Galina, N.S. Guermanov, M. Rykova, E. Antropova, O. Kutko, S. Shulguina, K. Orlova, V. A. Shmarov, E. A. Lysenko, S. Ponomarev","doi":"10.21687/0233-528x-2022-56-2-11-19","DOIUrl":"https://doi.org/10.21687/0233-528x-2022-56-2-11-19","url":null,"abstract":"Expression of inborn immunity genes was studied in 10 essentially healthy male subjects aged 24 to 32 years in a 21-day dry immersion experiment without countermeasures. To evaluate the humoral immunity function, genes encoding Toll-like receptors, proteins mediating NF-kB, AP-1, Akt, TLR4; apoptosis proteins, and CLEC4E, HSPD1, PTGS2 were selected. Fenol-cloroform extraction was used to obtain total RNA from peripheral blood monocytes CD14+. Subsequently, total RNA was reverse transcribed to generate cDNA for real-time PCR. The results showed substantial changes in expression of these genes suggesting that changes in the TLRs function on the transcriptional level were at the very start of the immune response to 21-d DI.","PeriodicalId":8683,"journal":{"name":"Aviakosmicheskaia i ekologicheskaia meditsina = Aerospace and environmental medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67951670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.21687/0233-528x-2022-56-3-40-46
A. Alkhaddur, E. V. Mashkina
Investigations of the effects of phitochemical compounds from extracts of pomegranate, grape seeds and garlic on expression of NFE2L2, JUN, SOD1 in cultivated cells of the human blood showed that transcription of SOD1 increased in the presence of pomegranate and grape seed extracts. Level of mRNA in NFE2L2 and JUN rose briskly only under the influence of a high concentration of the grape seed extract. Level of JUN mRNA correlated with NFE2L2 mRNA. Garlic extract had no effect on transcription of NFE2L2, JUN, SOD1. Activation of the transcription of all 3 genes under study by the grape seeds extract can contribute to controlling the free radical processes in human cells.
{"title":"EFFECT OF PHITOCHEMICAL EXTRACTS ON EXPRESSION OF GENES NFE2L2, JUN, SOD1 IN HUMAN CELLS","authors":"A. Alkhaddur, E. V. Mashkina","doi":"10.21687/0233-528x-2022-56-3-40-46","DOIUrl":"https://doi.org/10.21687/0233-528x-2022-56-3-40-46","url":null,"abstract":"Investigations of the effects of phitochemical compounds from extracts of pomegranate, grape seeds and garlic on expression of NFE2L2, JUN, SOD1 in cultivated cells of the human blood showed that transcription of SOD1 increased in the presence of pomegranate and grape seed extracts. Level of mRNA in NFE2L2 and JUN rose briskly only under the influence of a high concentration of the grape seed extract. Level of JUN mRNA correlated with NFE2L2 mRNA. Garlic extract had no effect on transcription of NFE2L2, JUN, SOD1. Activation of the transcription of all 3 genes under study by the grape seeds extract can contribute to controlling the free radical processes in human cells.","PeriodicalId":8683,"journal":{"name":"Aviakosmicheskaia i ekologicheskaia meditsina = Aerospace and environmental medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67952559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.21687/0233-528x-2022-56-1-55-61
I. A. Rozanov, P. G. Kuznetsova, A. O. Savinkina, D. Shved, O. Ryumin, E. Tomilovskaya, V. Gushchin
Usability and validity of a based on virtual reality (VR) psychological support program were tested in a 3-day dry immersion study with participation of female subjects. Test results advocate that VR can be beneficial for managing the psychophysiological reactions in the period of acute adaptation to dry immersion.
{"title":"PSYCHOLOGICAL SUPPORT USING VIRTUAL REALITY IN A STUDY WITH THREE-DAY DRY IMMERSION","authors":"I. A. Rozanov, P. G. Kuznetsova, A. O. Savinkina, D. Shved, O. Ryumin, E. Tomilovskaya, V. Gushchin","doi":"10.21687/0233-528x-2022-56-1-55-61","DOIUrl":"https://doi.org/10.21687/0233-528x-2022-56-1-55-61","url":null,"abstract":"Usability and validity of a based on virtual reality (VR) psychological support program were tested in a 3-day dry immersion study with participation of female subjects. Test results advocate that VR can be beneficial for managing the psychophysiological reactions in the period of acute adaptation to dry immersion.","PeriodicalId":8683,"journal":{"name":"Aviakosmicheskaia i ekologicheskaia meditsina = Aerospace and environmental medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67950199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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