Pub Date : 2022-01-01DOI: 10.21687/0233-528x-2022-56-2-39-45
A.V. Zatsepilin, A. V. Khomenko, V.N. Filatov, S. D. Chistov, V.V. Simukhin, Yu.Yu. Kisliakov
Acceleration and g-load values were calculated based on the objective control data records of supermaneuver flights. Pilots filled out. The authors propose methods of psychophysiological training substantiated by the results of analysis of pilots' entries in a questionnaire addressing some psychophysiological aspects of flying supermaneuver aircrafts.
{"title":"PSYCHOPHYSIOLOGICAL ASPECTS OF EXECUTING SUPERMANEUVER FLIGHTS","authors":"A.V. Zatsepilin, A. V. Khomenko, V.N. Filatov, S. D. Chistov, V.V. Simukhin, Yu.Yu. Kisliakov","doi":"10.21687/0233-528x-2022-56-2-39-45","DOIUrl":"https://doi.org/10.21687/0233-528x-2022-56-2-39-45","url":null,"abstract":"Acceleration and g-load values were calculated based on the objective control data records of supermaneuver flights. Pilots filled out. The authors propose methods of psychophysiological training substantiated by the results of analysis of pilots' entries in a questionnaire addressing some psychophysiological aspects of flying supermaneuver aircrafts.","PeriodicalId":8683,"journal":{"name":"Aviakosmicheskaia i ekologicheskaia meditsina = Aerospace and environmental medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67951265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.21687/0233-528x-2022-56-2-85-89
S. K. Soldatov, S. P. Dragan, A. Bogomolov, M. Pirozhkov, S.Yu. Skriabin, O.V. Gorodenko, I.A. Sinelnikov
The paper deals with analysis of the technical staff acoustic environment in modern aviation facilities. Measurements in 4 aerodrome sites were aimed to determine signal amplitude and temporal characteristics, spectral (octave band 2 to 16 000 Hz), and levels of total infrared, maximal (LА, dB) and equivalent sound (LАeq, dB). It was found that the aerodrome conditioner is a source of high-intensity wide-band noise with character and intensity dependent on the conditioner location and operation mode. Noise measurements on esplanade and work stations during flight preparation of a fighter-bomber, and in the hanger with the aerodrome conditioner on showed that the equivalent sound varied in the range from 97 to 114 dB. Health effects of this acoustic environment should be reduced using engineering controls and personal protectors.
{"title":"HYGIENIC ASPECTS OF THE NOISE GENERATED BY AERODROME FACILITIES","authors":"S. K. Soldatov, S. P. Dragan, A. Bogomolov, M. Pirozhkov, S.Yu. Skriabin, O.V. Gorodenko, I.A. Sinelnikov","doi":"10.21687/0233-528x-2022-56-2-85-89","DOIUrl":"https://doi.org/10.21687/0233-528x-2022-56-2-85-89","url":null,"abstract":"The paper deals with analysis of the technical staff acoustic environment in modern aviation facilities. Measurements in 4 aerodrome sites were aimed to determine signal amplitude and temporal characteristics, spectral (octave band 2 to 16 000 Hz), and levels of total infrared, maximal (LА, dB) and equivalent sound (LАeq, dB). It was found that the aerodrome conditioner is a source of high-intensity wide-band noise with character and intensity dependent on the conditioner location and operation mode. Noise measurements on esplanade and work stations during flight preparation of a fighter-bomber, and in the hanger with the aerodrome conditioner on showed that the equivalent sound varied in the range from 97 to 114 dB. Health effects of this acoustic environment should be reduced using engineering controls and personal protectors.","PeriodicalId":8683,"journal":{"name":"Aviakosmicheskaia i ekologicheskaia meditsina = Aerospace and environmental medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67951592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.21687/0233-528x-2022-56-3-61-65
S. I. Klimarev, Yu E Siniak, G. V. Kanatova
Process variables of an UHF sterilizer of atmospheric moisture condensate were identified by testing. Analysis of the water regeneration and UHF treatment system enabled upgrade of the UHF sterilizer and test bench, specify basic process variables of the sterilizer and define the temperature range to kill germs in condensate.
{"title":"EVALUATION OF THE UHF-ENERGY STERILIZING EFFECT ON ATMOSPHERIC MOISTURE CONDENSATE","authors":"S. I. Klimarev, Yu E Siniak, G. V. Kanatova","doi":"10.21687/0233-528x-2022-56-3-61-65","DOIUrl":"https://doi.org/10.21687/0233-528x-2022-56-3-61-65","url":null,"abstract":"Process variables of an UHF sterilizer of atmospheric moisture condensate were identified by testing. Analysis of the water regeneration and UHF treatment system enabled upgrade of the UHF sterilizer and test bench, specify basic process variables of the sterilizer and define the temperature range to kill germs in condensate.","PeriodicalId":8683,"journal":{"name":"Aviakosmicheskaia i ekologicheskaia meditsina = Aerospace and environmental medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67952790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.21687/0233-528x-2022-56-4-64-69
A.Yu. Ratushnyy, L. B. Buravkova
Mesenchymal stromal/stem cells (MSCs) are capable of multilinear differentiation and participate in tissue homeostasis including the remodeling and reparation processes. With aging these cells more and more often activatesenescence (cell aging) that alters cell functions and microenvironment which can be the reason for age-related pathologies. One of the features of senescent MSCs is thought to be a decline of multipotency that may limit their reparative functions in tissues. The paper presents a study of the MSCs osteogenic and adipogenic potential during replicative senescence. A decline of the MSCs adipogenic potential was associated with downregulation of PPARG,(a key transcription regulator of adipogenesis). There was evidence of reciprocal increasing of the osteogenic potential even though the several positive osteogenesis regulators (BMP2, BMP6, IGF1, IL1B) were downregulated. Besides, during differentiation matrix calcification was enhanced and osteoprotegerin concentration increased that can be an issue in context of atherosclerotic plaques calcification in elder people. Expression of key osteogenesis regulator RUNX2 and markers SPARC, SPP1, COL1A1, BGLAP remained stable during replicative senescence of MSCs.
{"title":"ПОТЕНЦИАЛ К ДИФФЕРЕНЦИРОВКЕ МЕЗЕНХИМАЛЬНЫХ СТРОМАЛЬНЫХ КЛЕТОК ПРИ РЕПЛИКАТИВНОМ СТАРЕНИИ","authors":"A.Yu. Ratushnyy, L. B. Buravkova","doi":"10.21687/0233-528x-2022-56-4-64-69","DOIUrl":"https://doi.org/10.21687/0233-528x-2022-56-4-64-69","url":null,"abstract":"Mesenchymal stromal/stem cells (MSCs) are capable of multilinear differentiation and participate in tissue homeostasis including the remodeling and reparation processes. With aging these cells more and more often activatesenescence (cell aging) that alters cell functions and microenvironment which can be the reason for age-related pathologies. One of the features of senescent MSCs is thought to be a decline of multipotency that may limit their reparative functions in tissues. The paper presents a study of the MSCs osteogenic and adipogenic potential during replicative senescence. A decline of the MSCs adipogenic potential was associated with downregulation of PPARG,(a key transcription regulator of adipogenesis). There was evidence of reciprocal increasing of the osteogenic potential even though the several positive osteogenesis regulators (BMP2, BMP6, IGF1, IL1B) were downregulated. Besides, during differentiation matrix calcification was enhanced and osteoprotegerin concentration increased that can be an issue in context of atherosclerotic plaques calcification in elder people. Expression of key osteogenesis regulator RUNX2 and markers SPARC, SPP1, COL1A1, BGLAP remained stable during replicative senescence of MSCs.","PeriodicalId":8683,"journal":{"name":"Aviakosmicheskaia i ekologicheskaia meditsina = Aerospace and environmental medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67953864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.21687/0233-528x-2022-56-3-25-32
N. V. Kuzmenko, N. Rubanova, M. G. Pliss, V. Tsyrlin
Age-related changes in hemodynamics and heart rate variability (HRV) as well as effects of low temperature and atmospheric pressure fluctuations on the cardiovascular functioning were investigated in normotensive Wistar rats of different age groups. In aging rats, decrease of systolic blood pressure (BPs) was not accompanied by considerable changes in heart rate (HR) or HRV. In none of the age groups a short exposure to the cold (30 min at 0 °С) caused stable changes in BPs and HR; however, recovery of the high-frequency HRV spectrum in 22-mo old rats took more time than in the other age groups. Atmospheric pressure fluctuations (10–15 mmHg) did not influence hemodynamics in 10-mo rats substantially. In 22-mo old rats, atmospheric pressure growth and decrease increased BPs. Besides, low atmospheric pressure was the reason of tachicardia and narrowing of the HRV high-frequency spectrum in this group. These data witness to the inhibition of vagus reactions to external environment stimuli in aged animals that may make them sensitive to climatic variations and compromise hemodynamics.
{"title":"ADAPTABILITY OF THE CARDIOVASCULAR SYSTEM IN NORMOTENSIVE RATS OF VARIOUS AGES AT FLUCTUATIONS OF AIR TEMPERATURE AND ATMOSPHERIC PRESSURE","authors":"N. V. Kuzmenko, N. Rubanova, M. G. Pliss, V. Tsyrlin","doi":"10.21687/0233-528x-2022-56-3-25-32","DOIUrl":"https://doi.org/10.21687/0233-528x-2022-56-3-25-32","url":null,"abstract":"Age-related changes in hemodynamics and heart rate variability (HRV) as well as effects of low temperature and atmospheric pressure fluctuations on the cardiovascular functioning were investigated in normotensive Wistar rats of different age groups. In aging rats, decrease of systolic blood pressure (BPs) was not accompanied by considerable changes in heart rate (HR) or HRV. In none of the age groups a short exposure to the cold (30 min at 0 °С) caused stable changes in BPs and HR; however, recovery of the high-frequency HRV spectrum in 22-mo old rats took more time than in the other age groups. Atmospheric pressure fluctuations (10–15 mmHg) did not influence hemodynamics in 10-mo rats substantially. In 22-mo old rats, atmospheric pressure growth and decrease increased BPs. Besides, low atmospheric pressure was the reason of tachicardia and narrowing of the HRV high-frequency spectrum in this group. These data witness to the inhibition of vagus reactions to external environment stimuli in aged animals that may make them sensitive to climatic variations and compromise hemodynamics.","PeriodicalId":8683,"journal":{"name":"Aviakosmicheskaia i ekologicheskaia meditsina = Aerospace and environmental medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67953010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.21687/0233-528x-2022-56-2-30-38
K. Lebedeva-Georgievskaya, A. Perevezentsev, O. Kuznetsova, V. Kudrin, V. Pikalov, O. Mitrofanova, A. A. Masanova, A. Shtemberg
Molecular mechanisms of the neurobiological effects of 10-d suspension combined with daily exposure to ᵞ-radiation and carbon ions were studied in rats. Analysis of the molecular changes in hippocamp dopamine receptors revealed changes in irradiated rats including, specifically, intensive expression of monoamine oxidase А, substrates for which are serotonin, adrenalin, noradrenaline and histamine. The EEG spectral and amplitude-frequency characteristics were analyzed. Comparison of primary EEG data regardless of rhythms brought to light a downward trend in the alpha frequencies. A similar trend was observed in the center frequencies and amplitudes of the most expressed peak, and the center frequency and amplitude of the delta highest peak. More anxious behavior and a somewhat quicker learning to fulfill the Morris tests and conditioned response of active avoidance in the shuttle chamber were in harmony with changes in metabolites of hippocamp dopamine and serotonin.
{"title":"MOLECULAR, NEUROPHYSIOLOGICAL AND INTEGRATIVE EFFECTS OF THE COMBINED EXPOSURE TO TAIL-SUSPENSION AND IONIZING RADIATION IN RATS","authors":"K. Lebedeva-Georgievskaya, A. Perevezentsev, O. Kuznetsova, V. Kudrin, V. Pikalov, O. Mitrofanova, A. A. Masanova, A. Shtemberg","doi":"10.21687/0233-528x-2022-56-2-30-38","DOIUrl":"https://doi.org/10.21687/0233-528x-2022-56-2-30-38","url":null,"abstract":"Molecular mechanisms of the neurobiological effects of 10-d suspension combined with daily exposure to ᵞ-radiation and carbon ions were studied in rats. Analysis of the molecular changes in hippocamp dopamine receptors revealed changes in irradiated rats including, specifically, intensive expression of monoamine oxidase А, substrates for which are serotonin, adrenalin, noradrenaline and histamine. The EEG spectral and amplitude-frequency characteristics were analyzed. Comparison of primary EEG data regardless of rhythms brought to light a downward trend in the alpha frequencies. A similar trend was observed in the center frequencies and amplitudes of the most expressed peak, and the center frequency and amplitude of the delta highest peak. More anxious behavior and a somewhat quicker learning to fulfill the Morris tests and conditioned response of active avoidance in the shuttle chamber were in harmony with changes in metabolites of hippocamp dopamine and serotonin.","PeriodicalId":8683,"journal":{"name":"Aviakosmicheskaia i ekologicheskaia meditsina = Aerospace and environmental medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67951259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.21687/0233-528x-2022-56-2-5-10
O. Ryumin, А.М. Voitenko
The paper speculates on selecting candidates for interplanetary crew commanders with the emphasis on must-have managerial qualities (MMQs) key for successful crew activities and control. The authors introduce the MMQs structure and approach to the MMQs assessment by the example of an AF command flight. Psychological training is essential for MMQs development and high dependability of interplanetary crew commanders. The training program comprises familiarization with theoretical grounds, practicing various scenarios of team work and commander's role of a manager, and concentration on skills required for accomplishment of a specific mission.
{"title":"MANAGERIAL PROFESSIONALLY IMPORTANT QUALITIES OF THE INTERPLANETARY CREW COMMANDER: STRUCTURE, ASSESSMENT, DEVELOPMENT","authors":"O. Ryumin, А.М. Voitenko","doi":"10.21687/0233-528x-2022-56-2-5-10","DOIUrl":"https://doi.org/10.21687/0233-528x-2022-56-2-5-10","url":null,"abstract":"The paper speculates on selecting candidates for interplanetary crew commanders with the emphasis on must-have managerial qualities (MMQs) key for successful crew activities and control. The authors introduce the MMQs structure and approach to the MMQs assessment by the example of an AF command flight. Psychological training is essential for MMQs development and high dependability of interplanetary crew commanders. The training program comprises familiarization with theoretical grounds, practicing various scenarios of team work and commander's role of a manager, and concentration on skills required for accomplishment of a specific mission.","PeriodicalId":8683,"journal":{"name":"Aviakosmicheskaia i ekologicheskaia meditsina = Aerospace and environmental medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67951320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.21687/0233-528x-2022-56-3-19-24
V. P. Nikolaev
Modified US navy Tables of decompression and stop times were used to provide theoretical grounds for reckoning of ascent rate to the surface after saturation dives to 500 on a heliox mixture lasting 18 days, 12 hrs and 35 minutes. According to calculation, dynamics of helium tension in the slowest body tissues points to oversaturation, the risk of gas bubble formation and development of decompression sickness symptoms. This risk can be averted fully by decelerating the standard ascent rate in half.
{"title":"SCHEDULE OF ASCENT TO THE SURFACE AFTER SATURATION DIVE ON HELIOX MIXTURE TO 500 METERS","authors":"V. P. Nikolaev","doi":"10.21687/0233-528x-2022-56-3-19-24","DOIUrl":"https://doi.org/10.21687/0233-528x-2022-56-3-19-24","url":null,"abstract":"Modified US navy Tables of decompression and stop times were used to provide theoretical grounds for reckoning of ascent rate to the surface after saturation dives to 500 on a heliox mixture lasting 18 days, 12 hrs and 35 minutes. According to calculation, dynamics of helium tension in the slowest body tissues points to oversaturation, the risk of gas bubble formation and development of decompression sickness symptoms. This risk can be averted fully by decelerating the standard ascent rate in half.","PeriodicalId":8683,"journal":{"name":"Aviakosmicheskaia i ekologicheskaia meditsina = Aerospace and environmental medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67952995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.21687/0233-528x-2022-56-6-37-42
V. Ilyin, L. Starkova
The existing deep diving statistics shows that hyperbaria consequences to the human health increase as the dive gets deeper and longer. The most common reason for termination of occupational saturation dives and early decompression is mycotic otitis externa and otitis media. In 1980s IBMP carried out investigations of the microbial status of deep saturation divers working at various depths. The results point to the fact that regardless of diver's biotope from the beginning of the isopression phase, the commensal microflora decreases gradually and the count of opportunistic gram-negative bacteria rises. Administration of antibiotics during prolonged dives must be restricted as an accessory selection factor for resistant agent.
{"title":"MICROBIAL STATUS OF DEEP DIVERS","authors":"V. Ilyin, L. Starkova","doi":"10.21687/0233-528x-2022-56-6-37-42","DOIUrl":"https://doi.org/10.21687/0233-528x-2022-56-6-37-42","url":null,"abstract":"The existing deep diving statistics shows that hyperbaria consequences to the human health increase as the dive gets deeper and longer. The most common reason for termination of occupational saturation dives and early decompression is mycotic otitis externa and otitis media. In 1980s IBMP carried out investigations of the microbial status of deep saturation divers working at various depths. The results point to the fact that regardless of diver's biotope from the beginning of the isopression phase, the commensal microflora decreases gradually and the count of opportunistic gram-negative bacteria rises. Administration of antibiotics during prolonged dives must be restricted as an accessory selection factor for resistant agent.","PeriodicalId":8683,"journal":{"name":"Aviakosmicheskaia i ekologicheskaia meditsina = Aerospace and environmental medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67956654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.21687/0233-528x-2022-56-6-70-78
P.D. Osipova, D. Karpov, A. Domashin, N. Polyakov, A. Soloviev, M. Zubasheva, V. Zhukhovitsky, S. Poddubko
Spore-forming bacteria are residents on the International space station (ISS) and can act as potential biodistructors of structural materials with a consequent disturbance of equipment operation. These bacteria demonstrate high resistance to many stress factors. Molecular mechanisms of this resistance are poorly understood. Sanitary/hygienic monitoring resulted in detection and isolation of strain Bacillus pumilus 25. The investigation was aimed to test B. pumilus 25 resistance to oxidative agents, antibiotics, and to analyze expression of genes associated with the cell response to DNA damage and hydrogen sulfide production. The strain was identified by sequencing 16S RNA and MALDI-TOF analysis. Resistance to stresses and antibiotics was tested with the use of standard microbiological methods. Level of mRNA genes was determined with the real-time polymerase chain reaction. Our results evidence for heightened B. pumilus 25 resistance to the oxidative stress and majority of the antibiotics used in the investigation. This resistance seems to be associated with high expression of the ku-ligD operon genes, and elevated production of hydrogen sulfide.
{"title":"MOLECULAR MECHANISMS OF BACILLUS PUMILUS 25 SUPER RESISTANCE TO ANTIBIOTICS AND OXIDATIVE STRESS","authors":"P.D. Osipova, D. Karpov, A. Domashin, N. Polyakov, A. Soloviev, M. Zubasheva, V. Zhukhovitsky, S. Poddubko","doi":"10.21687/0233-528x-2022-56-6-70-78","DOIUrl":"https://doi.org/10.21687/0233-528x-2022-56-6-70-78","url":null,"abstract":"Spore-forming bacteria are residents on the International space station (ISS) and can act as potential biodistructors of structural materials with a consequent disturbance of equipment operation. These bacteria demonstrate high resistance to many stress factors. Molecular mechanisms of this resistance are poorly understood. Sanitary/hygienic monitoring resulted in detection and isolation of strain Bacillus pumilus 25. The investigation was aimed to test B. pumilus 25 resistance to oxidative agents, antibiotics, and to analyze expression of genes associated with the cell response to DNA damage and hydrogen sulfide production. The strain was identified by sequencing 16S RNA and MALDI-TOF analysis. Resistance to stresses and antibiotics was tested with the use of standard microbiological methods. Level of mRNA genes was determined with the real-time polymerase chain reaction. Our results evidence for heightened B. pumilus 25 resistance to the oxidative stress and majority of the antibiotics used in the investigation. This resistance seems to be associated with high expression of the ku-ligD operon genes, and elevated production of hydrogen sulfide.","PeriodicalId":8683,"journal":{"name":"Aviakosmicheskaia i ekologicheskaia meditsina = Aerospace and environmental medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67957433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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