Biochemical Engineering Journal最新文献_第2页

Competitive and synergistic effects of manganese-nitrogen co-doped sludge biochar on the chlortetracycline hydrochloride and Cu²⁺ from water 锰氮共掺杂污泥生物炭对盐酸氯四环素和水中Cu 2 +的竞争和协同效应

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-01-15 DOI: 10.1016/j.bej.2026.110082

Xinhua Zhou, Zhihao Li, Ziqiang Ding, Lin Lu, Letong Wang

Addressing the issue of simultaneous antibiotic and heavy metal contamination in effluent from intensive animal husbandry, this study used sewage sludge as the raw material. It employed co-doping modification with urea and manganese chloride to synthesize a sludge biochar composite (Mn-N-SBC) through pyrolysis for removing CTC and Cu^2 + from aquaculture effluent. Experimental results showed that in the single system, the Qemax values were 93.27 mg/g and 31.45 mg/g, respectively. In the binary system, the Qemax values were 94.89 mg/g and 30.76 mg/g, respectively. These findings indicate that competing interactions and synergistic effects coexist during the concurrent removal of CTC and Cu²⁺ by Mn-N-SBC. Microstructural analysis suggests that the competitive adsorption of CTC and Cu²⁺ on Mn-N-SBC mainly results from their competition for the same active sites. This enhanced adsorption can be attributed to electrostatic interactions, Cu²⁺ bridging effects, and interactions involving O/N functional groups within CTC molecules. Overall, this study clarifies the concurrent adsorption mechanism of Mn-N-SBC for CTC and Cu²⁺, offering both a strategy for sludge valorization and a template for designing multifunctional adsorbents to treat complex wastewater.

针对集约化畜牧业废水中同时存在抗生素和重金属污染的问题，本研究以污水污泥为原料。采用尿素和氯化锰共掺杂改性，热解合成污泥生物炭复合材料（Mn-N-SBC），用于去除水产养殖出水中的CTC和Cu2 +。实验结果表明，在单一体系中，Qemax值分别为93.27 mg/g和31.45 mg/g。在二元体系中，Qemax值分别为94.89 mg/g和30.76 mg/g。这些结果表明，在Mn-N-SBC同时去除CTC和Cu2+的过程中，竞争相互作用和协同效应并存。微观结构分析表明，CTC和Cu2+在Mn-N-SBC上的竞争吸附主要是由于它们对相同活性位点的竞争。这种增强的吸附可归因于静电相互作用、Cu2+桥接效应和CTC分子内O/N官能团的相互作用。总的来说，本研究阐明了Mn-N-SBC对CTC和Cu2+的同时吸附机理，为污泥增值策略和设计多功能吸附剂处理复杂废水提供了模板。

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引用次数: 0

Configuration and optimization of in-situ microbial protein production via bio-electrochemical system 生物电化学原位微生物蛋白生产系统的配置与优化

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-01-12 DOI: 10.1016/j.bej.2026.110081

Yilin Jiang , Hangyu Sun , Shimin Wu , Xiangjie Xiao , Yanping Liu , Ziyi Yang

Upcycling of nitrogen for producing microbial protein (MP) is a promising method to support the development of circular economy. In this study, in-situ MP production system was upgraded based on microbial electrolytic cell (MEC). Firstly, ammonia-tolerant electroactive microorganisms were enriched on the bio-anode electrode at 1500 mg N/L. Secondly, to enhance the NH₄⁺ -N migration efficiency, ion concentration in the cathode chamber of 20 mmol K₂HPO₄ + 20 mmol Na₂HPO₄, pH in the cathode chamber of 7.0 and applied voltage of 0.6 V was selected. Thirdly, in-situ MP production system showed the feasibility, with MP production of 1.82 g/L. Finally, voltage of 1.0 V, NH₄⁺-N concentration of 1 g N/L in anode chamber and 20°C were selected as the optimal operational conditions. And MP production of 2.19 g/L was obtained, with 30.47 % of nitrogen stored in MP. Essential amino acids of MP produced in this study was about 42 %, belonging to the high-quality proteins. In-situ protein production system showed the promising MP synthesis capacity, which could be used as a sustainable solution for nitrogen upcycling.

氮的升级回收生产微生物蛋白是支持循环经济发展的一种很有前途的方法。本研究对基于微生物电解池（MEC）的原位MP生产系统进行了升级。首先，在1500 mg N/L的生物阳极电极上富集耐氨电活性微生物。其次，为提高NH4+ -N迁移效率，阴极室离子浓度为20 mmol K2HPO4 + 20 mmol Na2HPO4，阴极室pH为7.0，施加电压为0.6 V。第三，原位生产MP系统显示出可行性，MP产量为1.82 g/L。最终选择电压为1.0 V，阳极室NH4+-N浓度为1 g N/L，温度为20℃为最佳操作条件。MP的产量为2.19 g/L，其中30.47 %的氮储存在MP中。本研究生产的MP必需氨基酸含量约为42% %，属于优质蛋白质。原位蛋白质生产系统显示出良好的MP合成能力，可作为氮升级循环的可持续解决方案。

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引用次数: 0

Lignin valorization to polyhydroxyalkanoates via microbial co-cultured and genome reduction method 微生物共培养和基因组还原法研究木质素转化为聚羟基烷酸酯

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-01-12 DOI: 10.1016/j.bej.2026.110080

Yi-Fei Zhang , Dong Wang , Yin-Ping Wang , Zhong-Wei Fan , Jian Liu , Shi-Lei Yu , Jia-Qi Cui

The utilization of agricultural waste as a renewable carbon source for biopolymer production represents a critical component of the circular economy. To enhance the bioconversion efficiency of bio-based polymers, a co-cultured system and a genome reduction method were employed to improve lignin valorization. Pseudomonas putida KT2440 exhibited higher lignin bioconversion capacity than other evaluated strains. When co-cultured with Bacillus sp. S0, the dry cell weight (DCW) and polyhydroxyalkanoate (PHA) concentration increased to 1.6 g/L and 42.6 mg/L, respectively, compared with P. putida KT2440 alone. Lignin valorization was further enhanced when the genome-reduced strain P. putida KTU-13 was co-cultured with Bacillus sp. S0. After optimizing key fermentation parameters, including nitrogen content, inoculation rate, and strain ratio, the DCW and PHA concentration reached 2.0 g/L and 67.1 mg/L, respectively. Overall, this work demonstrates a sustainable approach for improving bio-based polymer production through integrated process optimization.

利用农业废弃物作为生物聚合物生产的可再生碳源是循环经济的重要组成部分。为了提高生物基聚合物的生物转化效率，采用共培养体系和基因组还原方法来改善木质素的增值。恶臭假单胞菌KT2440表现出较高的木质素转化能力。与枯草芽孢杆菌KT2440共培养时，枯草芽孢杆菌的干细胞重（DCW）和聚羟基烷酸酯（PHA）浓度分别提高到1.6 g/L和42.6 mg/L。当基因组减少的恶臭杆菌KTU-13与芽孢杆菌S0共培养时，木质素的增殖能力进一步增强。对氮含量、接种量、菌种比等关键发酵参数进行优化后，DCW和PHA浓度分别达到2.0 g/L和67.1 mg/L。总的来说，这项工作证明了通过集成工艺优化改善生物基聚合物生产的可持续方法。

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引用次数: 0

Corrigendum to “Use of free nitrous acid from partial nitrification reactor for the sanitization of digester effluents and Class A biosolids production” [Biochem. Eng. J. 226 (2026) 109993] 部分硝化反应器游离亚硝酸盐用于消化废水的消毒和A类生物固体生产的更正[生物化学]。Eng。J. 226 (2026) 109993]

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-01-12 DOI: 10.1016/j.bej.2026.110077

Zeinab Morhell , Gabriel Martínez , Oscar Franchi , Dafne Crutchik , Cristofer Da Silva , Lorna Guerrero , Alba Pedrouso , Ángeles Val del Río , Anuska Mosquera-Corral , José Luis Campos

引用次数: 0

Membrane transporter mining and enzyme engineering drive high-titer δ-tocotrienol production in Yarrowia lipolytica 膜转运体的挖掘和酶工程驱动高滴度的产脂耶氏菌δ-生育三烯醇

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-01-09 DOI: 10.1016/j.bej.2026.110079

Chenchen Xu , Xinyu Jiao , Quanlu Zhao , Tianli Yuan , Haoran Bi , Kai Wang , Tianwei Tan

δ-Tocotrienol, a vitamin E bioactive isoform, possesses strong antioxidant and anticancer properties. However, its production is limited by its extremely low abundance in plants and the difficulty of separating it from other tocotrienol isomers. Therefore, in this study, Y. lipolytica was engineered for high-level δ-tocotrienol production using a multipronged strategy. Heterologous δ-tocotrienol pathway gene expression and precursor supply enhancement facilitated the production of 2.8 mg/L δ-tocotrienol. Transcriptomic analysis and metabolic modeling (iYALI743) identified four transporters that markedly improved the δ-tocotrienol titer to 4.68 mg/L. Moreover, PpHPD and SyHPT protein fusion, and the truncation of the N-terminal signal peptide of AtTC, which enhanced plant enzyme performance in Y. lipolytica, raised the δ-tocotrienol titer to 54.67 mg/L. Under optimized fermentation conditions with antioxidant butylated hydroxytoluene (BHT) supplementation, the engineered strain achieved a δ-tocotrienol titer of 617.23 mg/L in a 2 L bioreactor, representing the highest yield on record.

δ-生育三烯醇是维生素E的一种生物活性异构体，具有很强的抗氧化和抗癌特性。然而，由于其在植物中的丰度极低，并且难以从其他生育三烯醇异构体中分离出来，其生产受到限制。因此，在本研究中，利用多管齐下的策略，对聚脂酵母进行了高水平的δ-生育三烯醇生产。异源δ-生育三烯醇途径基因的表达和前体供应的增加促进了2.8 mg/L δ-生育三烯醇的产生。转录组学分析和代谢模型（iYALI743）鉴定出四种转运蛋白，显著提高了δ-生育三烯醇滴度至4.68 mg/L。此外，PpHPD与SyHPT蛋白融合，截断AtTC的n端信号肽，增强了植物酶在脂聚Y. lipolytica中的表现，使δ-生育三烯醇滴度提高到54.67 mg/L。在添加抗氧化剂丁基羟基甲苯（BHT）的优化发酵条件下，工程菌株在2 L的生物反应器中获得了617.23 mg/L的δ-生育三烯醇滴度，这是有记录以来的最高产量。

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引用次数: 0

A novel multi-directional modular mixing system for bringing new insight to microalgae production in panel photobioreactors 一种新型的多向模块化混合系统，为面板光生物反应器中的微藻生产带来了新的见解

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-01-08 DOI: 10.1016/j.bej.2026.110074

Barış Erdoğan , S. Furkan Demi̇rden , Deniz Senyay-Oncel , Suphi S. Oncel

A novel system was designed herein to enhance the mixing efficiency of conventional panel photobioreactors (PBRs). Initially, design optimization was conducted using three-dimensional computational modeling. For this, CFD simulations performed using the Shear Stress Transport model of different mixing configurations. These are single vertical shaft (1B), single turbine on each lateral shafts (2B) and single turbines in all directions (3B), respectively. Rushton and marine type impellers are used in these simulations for comprehensive evaluation. Here, Chlamydomonas reinhardtii CC-124 was used as model microalgae. In both simulations and validation studies Rushton turbine gives better results (36.81 ± 0.23 mg.L⁻¹) when compared with marine impeller (16.42 ± 0.67 mg.L⁻¹) in terms of highest total chlorophyll amount reached. Among the different configurations with Rushton impeller, the 1B and 3B configurations come forward. Although, 3B reached higher average shear stress value (3.63 Pa) than 1B, this configuration was able to reach higher microalgae concentration in a short time during the 13-day culture period when evaluated in terms of biomass. This result indicates that 3B configuration which creates highest magnitude multidirectional flow vectors provides a consistent homogeneous mixing for better biomass production in PBR. Based on these results, it can be said that this modular mixing system design is a promising contribution for panel PBRs and new microalgae production systems.

为了提高面板型光生物反应器（PBRs）的混合效率，设计了一种新型系统。最初，采用三维计算建模进行设计优化。为此，采用不同混合配置的剪切应力输运模型进行CFD模拟。它们分别是单垂直轴（1B），每侧轴上的单涡轮（2B）和所有方向上的单涡轮（3B）。在这些模拟中使用了Rushton和marine型叶轮进行综合评价。本文以莱茵衣藻CC-124为模型微藻。在模拟和验证研究中，Rushton涡轮的最高叶绿素含量（36.81 ± 0.23 mg.L−1）优于船用叶轮（16.42 ± 0.67 mg.L−1）。在Rushton叶轮的不同配置中，提出了1B和3B两种配置。虽然3B的平均剪应力值（3.63 Pa）高于1B，但从生物量的角度来看，在13天的培养期内，这种配置能够在短时间内达到更高的微藻浓度。该结果表明，产生最大量级多向流矢量的3B配置为PBR中更好的生物质生产提供了一致的均匀混合。基于这些结果，可以说这种模块化混合系统设计对板式pbr和新的微藻生产系统有很大的贡献。

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引用次数: 0

Flocculation process performance evaluation of microalgae grown with different organic carbon sources and recycling of spent medium 不同有机碳源培养微藻絮凝工艺性能评价及废媒循环利用

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-01-08 DOI: 10.1016/j.bej.2026.110076

Dian Dai , Li Feng , Qing Li , Sirui Lv , Zhe Zhang , Ruicheng Yang , Zhangfeng Hu , Liandong Zhu

Incorporating organic carbon sources into the growth medium serves as an effective method for promoting microalgal productivity. Importantly, variations in carbon metabolism significantly impact the efficiency of subsequent biomass harvesting processes. There is a clear necessity to evaluate and select carbon sources that enable large-scale microalgae growth while preserving the efficiency of subsequent biomass separation. In this study, three representative flocculants (alum, CPAM: cationic polyacrylamide, and CS: chitosan) were utilized to evaluate the flocculation performance of microalgae cultivated with diverse carbon sources. Furthermore, by monitoring fundamental physiological parameters of microalgae, the feasibility of spent medium following harvesting with different flocculants was systematically evaluated. The research findings indicated that the flocculation performance of sodium acetate‑cultured microalgae was inhibited when harvested with alum or CS, primarily due to excessive extracellular protein secretion. Ethanol can be considered the optimal carbon source choice. While markedly enhancing microalgal biomass, its use in conjunction with alum, CPAM, or CS enables efficient harvesting, outperforming other carbon sources. When microalgae were recultured in a solution containing 50 % recycled spent medium, minimal impact was observed on fundamental physiological indicators, with only a slight reduction in fatty acid unsaturation detected. This study provided valuable insights into the selection of appropriate organic carbon sources for promoting microalgal harvesting and clarified the spent medium after harvesting utilization strategies.

在生长培养基中加入有机碳源是提高微藻产量的有效方法。重要的是，碳代谢的变化显著影响后续生物质收获过程的效率。显然有必要评估和选择碳源，使微藻能够大规模生长，同时保持后续生物质分离的效率。本研究采用明矾、CPAM：阳离子聚丙烯酰胺和CS：壳聚糖三种具有代表性的絮凝剂，对不同碳源培养的微藻絮凝性能进行了评价。此外，通过监测微藻的基本生理参数，系统评估了不同絮凝剂收获后废培养基的可行性。研究结果表明，当明矾或CS收获时，醋酸钠培养微藻的絮凝性能受到抑制，这主要是由于细胞外蛋白分泌过多。乙醇可以被认为是最佳的碳源选择。在显著提高微藻生物量的同时，它与明矾、CPAM或CS结合使用可以实现高效收获，优于其他碳源。当微藻在含有50% %再生废培养基的溶液中再培养时，对基本生理指标的影响最小，仅检测到脂肪酸不饱和略有减少。本研究为促进微藻收获的有机碳源选择提供了有价值的见解，并阐明了收获后废培养基的利用策略。

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引用次数: 0

Low-cost solid-state fermentation of lipopeptides by Staphylococcus sp. DG-2 for petroleum-contaminated soil remediation 葡萄球菌DG-2低成本固态发酵脂肽修复石油污染土壤

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-01-08 DOI: 10.1016/j.bej.2026.110078

Quanli Man, Linhao Kang, Hanjie Zu, Zhineng Wu, Xiaodong Ma

This study reports a novel petroleum-degrading strain of Staphylococcus sp. that addresses the challenges of high costs and low productivity in lipopeptide (LP) production. Strain DG-2 produces LP through solid-state fermentation using low-cost agricultural waste, specifically soybean meal. FTIR, HPLC-MS, and surface tension analyses confirmed the LP structure as CH₃-(CH₂)₁₇-CHO-CH₂-CO-Gly-Gly-Gly-Leu-Met-Leu-Leu, with a critical micelle concentration (CMC) of 160 mg/L, effectively reducing the surface tension to 26.9 mN/m while maintaining stability across a pH range of 8–12 and temperatures of 20–80°C. Under optimized SSF conditions (30°C, 6 d, 12.9 g soybean meal, 1.3 g/L MgSO₄·7H₂O, 0.38 g/L FeSO₄), response surface methodology optimization achieved a remarkable LP concentration of 49.5 mg/g ds, representing a 25.8 % increase from initial conditions. Notably, DG-2 demonstrated 52.5 % degradation of C13-C26 alkanes and 71.1 % removal of 2–4 ring PAHs in crude oil. Under optimal washing conditions (0.2 g/L LP, water-soil ratio 10:1, 65°C, 60 min), a total petroleum hydrocarbon (TPH) removal of 53.0 % was achieved from heavily petroleum-contaminated soil (8.0 % TPH). These findings demonstrated that DG-2 is an exceptional candidate for petroleum bioremediation, offering both a high-performance microbial resource and an optimized low-cost production strategy with significant industrial potential.

本研究报道了一种新的石油降解葡萄球菌，该菌株解决了脂肽（LP）生产中高成本和低生产率的挑战。菌株DG-2利用低成本的农业废弃物，特别是豆粕，通过固态发酵生产LP。FTIR， HPLC-MS和表面张力分析证实LP结构为CH3-(CH2)17-CHO-CH2-CO-Gly-Gly-Gly-Leu-Met-Leu-Leu，临界胶束浓度（CMC）为160 mg/L，有效降低表面张力至26.9 mN/m，同时在8-12的pH范围和20-80°C的温度范围内保持稳定性。在优化的SSF条件下（30°C, 6 d， 12.9 g豆粕，1.3 g/L MgSO4·7H2O, 0.38 g/L FeSO4），响应面法优化得到的LP浓度为49.5 mg/g ds，比初始条件提高25.8%。值得注意的是，DG-2对原油中C13-C26烷烃的降解率为52.5%，对2-4环多环芳烃的去除率为71.1%。在最佳洗涤条件（0.2 g/L LP，水土比10:1,65℃，60 min）下，重石油污染土壤（TPH为8.0%）的总石油烃（TPH）去除率为53.0%。这些发现表明，DG-2是石油生物修复的一个特殊候选者，既提供了高性能的微生物资源，又提供了优化的低成本生产策略，具有巨大的工业潜力。

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引用次数: 0

Amelioration of mining-area soils with microbial induction of magnesium ammonium phosphate 微生物诱导磷酸铵镁改良矿区土壤

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-01-07 DOI: 10.1016/j.bej.2026.110075

Zhe Wang , Wen-yan Zhang , Jun-li Cheng , Shu-hui Wei , Hong-yuan Cui , Wei-da Wang

Large-scale mineral exploitation causes heavy metal pollution and nutrient depletion in mining-area soils, making heavy metal control and soil fertility improvement urgent. This study synthesized biological magnesium ammonium phosphate (bio-MAP) via microbially induced mineralization, characterized its microstructure, and conducted pot experiments with ryegrass and alfalfa under different bio-MAP additions to analyze effects on soil physicochemical properties, Pb²⁺/Cd²⁺ contents, and plant growth. X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) confirmed the precipitate was mainly struvite (MgNH₄PO₄·6H₂O) with a regular orthorhombic structure. Appropriate bio-MAP addition not only facilitated the formation of large soil aggregates and enhanced soil structural stability but also significantly promoted the growth of ryegrass and alfalfa. At the 30-day harvest, the total fresh weight of ryegrass reached 1.33 g in the P5 treatment (bio-MAP addition: 1000 mg/kg), while that of alfalfa reached 2.36 g in the P3 treatment (bio-MAP addition: 500 mg/kg). Compared with the control, the P1, P3, and P5 treatments reduced soil available Pb content by 7.16 %, 15.1 %, and 19.28 %, respectively, and soil available Cd content by 58.57 %, 62.31 %, and 65.1 %, respectively. Bio-MAP also increased soil sucrase activity and abundances of Proteobacteria, Actinobacteria, and Bacillus. This study provides a new method for mine soil amendment and heavy metal control, plus references for ecological restoration.

大规模矿产开采造成矿区土壤重金属污染和养分耗竭，治理重金属和提高土壤肥力刻不容缓。本研究通过微生物诱导矿化法合成了生物磷酸镁铵（bio-MAP），对其微观结构进行了表征，并在不同生物map添加量的黑麦草和苜蓿上进行了盆栽实验，分析了生物map添加量对土壤理化性质、Pb 2 + /Cd 2 +含量以及植物生长的影响。x射线衍射（XRD）、傅里叶变换红外光谱（FTIR）和扫描电镜（SEM）证实沉淀主要为鸟粪石（MgNH₄PO₄·6H₂O），具有规则的正交结构。适当添加生物map不仅有利于土壤团聚体的形成，增强土壤结构稳定性，而且显著促进黑麦草和苜蓿的生长。收获30 d时，P5处理（bio-MAP添加量为1000 mg/kg）黑麦草总鲜重达到1.33 g， P3处理（bio-MAP添加量为500 mg/kg）紫花苜蓿总鲜重达到2.36 g。与对照相比，P1、P3和P5处理土壤有效Pb含量分别降低了7.16 %、15.1 %和19.28 %，土壤有效Cd含量分别降低了58.57 %、62.31 %和65.1% %。Bio-MAP还增加了土壤蔗糖酶活性和变形菌、放线菌和芽孢杆菌的丰度。该研究为矿山土壤修复和重金属控制提供了新的方法，并为生态修复提供了参考。

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引用次数: 0

High-efficiency PHB production in Synechocystis salina M8 through sequential screening and optimization of bioprocess parameters salina Synechocystis M8高效产PHB生物工艺参数的筛选与优化

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-01-06 DOI: 10.1016/j.bej.2026.110073

Nguyen Thi Phuong Dung , Ta Thi Minh Anh , Bui Thi Thu Uyen , Luu Thi Thu Ha , Dao Duy Khanh , Tran Dang Thuan , Tran Huu Quang , Phan Quang Thang

Polyhydroxybutyrate (PHB) production by cyanobacteria represents a promising pathway toward net-zero CO₂ emissions and circular bioeconomy. This study evaluated a newly isolated strain, Synechocystis salina M8, to determine how key environmental factors regulate biomass accumulation and PHB synthesis. Using a Plackett–Burman design, light intensity (4500–13,500 lux), pH (5–9), and temperature (25–35 °C) were identified as the most influential parameters for biomass growth, while PHB accumulation was significantly affected only by pH. A subsequent Box–Behnken optimization defined the conditions that maximized strain performance: a light intensity of 9773 lux, pH 8.07, and temperature 31.97 °C. Under these optimized conditions, S. salina M8 achieved a high dry biomass concentration of 2.73 g L⁻¹ , with PHB content reaching 21.41 % of dry biomass, reflecting efficient intracellular biopolymer accumulation alongside robust growth. Beyond PHB production, the strain displayed strong environmental functionality, exhibiting substantial inorganic carbon assimilation (3.06–4.06 %) and remarkable nutrient removal efficiencies for phosphate (94.2–99.8 %) and nitrate (87.3–96.1 %). These attributes highlight its suitability for integrated CO₂ sequestration and wastewater bioremediation. Overall, the results demonstrate that precise environmental tuning is crucial for optimizing both biomass productivity and PHB yield. This study provides a practical framework for scaling cyanobacterial cultivation toward sustainable PHB production, supporting broader applications in circular bioeconomy development and climate mitigation strategies.

蓝藻生产聚羟基丁酸盐（PHB）是实现净零二氧化碳排放和循环生物经济的有希望的途径。本研究对一株新分离菌株salina Synechocystis M8进行了评价，以确定关键环境因子如何调节生物量积累和PHB合成。采用Plackett-Burman设计，确定光强（4500-13,500 lux）、pH（5-9）和温度（25-35°C）是影响生物量生长的最重要参数，而PHB积累仅受pH的显著影响。随后的Box-Behnken优化确定了菌株性能最大化的条件：光强9773 lux、pH 8.07和温度31.97°C。在这些优化条件下，S. salina M8获得了2.73 g L⁻¹ 的高干生物量浓度，PHB含量达到了干生物量的21.41 %，反映了细胞内生物聚合物的高效积累和强劲的生长。除生产PHB外，该菌株还表现出较强的环境功能，具有较强的无机碳同化能力（3.06-4.06 %），对磷酸盐（94.2-99.8 %）和硝酸盐（87.3-96.1 %）的去除效率。这些特性突出了它对二氧化碳固存和废水生物修复的综合适用性。总体而言，研究结果表明，精确的环境调节对于优化生物量生产力和PHB产量至关重要。该研究为蓝藻培养向可持续PHB生产的方向发展提供了一个实用框架，支持在循环生物经济发展和气候减缓战略中的更广泛应用。

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引用次数: 0