Biochemical Engineering Journal最新文献_第2页

Optimizing bamboo biomass for sustainable isobutanol production using genetically engineered Escherichia coli

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-02-11 DOI: 10.1016/j.bej.2025.109669

Said Nawab , Zahoor , Syed Bilal Shah , Mujeeb Ur Rahman , Hareef Ahmed Keerio , Inamur Rahman

Researchers are finding sustainable fuel alternatives due to the growing costs and environmental issues related to fossil energy. Isobutanol is an attractive biofuel with a higher energy content than ethanol and the potential to replace gasoline. Bamboo emerges as a promising and cost-effective feedstock for isobutanol production due to its fast growth and high holocellulosic content. By using response surface methodology, this study optimized glucose recovery from bamboo biomass by varying pretreatment temperature (30–70°C), reaction time (0.5–6 h), and NaOH concentration (0.5–3 %). The maximum glucose recovery was achieved using 1 % NaOH at 68°C for 6 hours, resulting in improved cellulose and reduced hemicellulose and lignin content in bamboo. This process released 31.01 mg/mL glucose, representing 65 % of available sugars. Structural modifications of the untreated and alkali-treated bamboo biomass were confirmed through Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). Isobutanol fermentation with the engineered E. coli strain JCL260 produced 2.28 g/L of isobutanol from enzymatic hydrolysate, achieving 22.86 % of the theoretical maximum. This study determines that cellulose from bamboo has been efficiently transformed into isobutanol through fermentation, although at a lower concentration associated with pure glucose.

{"title":"Optimizing bamboo biomass for sustainable isobutanol production using genetically engineered Escherichia coli","authors":"Said Nawab , Zahoor , Syed Bilal Shah , Mujeeb Ur Rahman , Hareef Ahmed Keerio , Inamur Rahman","doi":"10.1016/j.bej.2025.109669","DOIUrl":"10.1016/j.bej.2025.109669","url":null,"abstract":"<div><div>Researchers are finding sustainable fuel alternatives due to the growing costs and environmental issues related to fossil energy. Isobutanol is an attractive biofuel with a higher energy content than ethanol and the potential to replace gasoline. Bamboo emerges as a promising and cost-effective feedstock for isobutanol production due to its fast growth and high holocellulosic content. By using response surface methodology, this study optimized glucose recovery from bamboo biomass by varying pretreatment temperature (30–70°C), reaction time (0.5–6 h), and NaOH concentration (0.5–3 %). The maximum glucose recovery was achieved using 1 % NaOH at 68°C for 6 hours, resulting in improved cellulose and reduced hemicellulose and lignin content in bamboo. This process released 31.01 mg/mL glucose, representing 65 % of available sugars. Structural modifications of the untreated and alkali-treated bamboo biomass were confirmed through Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). Isobutanol fermentation with the engineered <em>E. coli</em> strain JCL260 produced 2.28 g/L of isobutanol from enzymatic hydrolysate, achieving 22.86 % of the theoretical maximum. This study determines that cellulose from bamboo has been efficiently transformed into isobutanol through fermentation, although at a lower concentration associated with pure glucose.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"217 ","pages":"Article 109669"},"PeriodicalIF":3.7,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143422730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation and study of CDs-WO3 composites with enhanced photocatalytic antimicrobial properties and degradation of dyes

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-02-11 DOI: 10.1016/j.bej.2025.109670

Qing Wang , Guanbin Wen , Zhengfang Yang , Qianqian Guo , Bin Zhang , Yanli Nie , Dongquan Wang

In this study, carbon dots (CDs) and tungsten trioxide (WO₃) were combined to prepare CDs-WO₃ composites for photocatalytic treatment of bacteria and organic dyes in wastewater. The optical properties, antimicrobial properties, antimicrobial mechanism, and photocatalytic degradation performance were also investigated. The CDs-WO₃ composites exhibited enhanced UV absorption intensity, broadened visible light absorption range, good charge transfer and carrier separation abilities, and reduced bandgap. The antimicrobial rate of CDs-WO₃ against Staphylococcus aureus can reach 98 %. The antibacterial mechanism of CDs-WO₃ was found to be the reactive oxygen species damage mechanism. Electron paramagnetic resonance (EPR) analysis revealed that the reactive oxygen species signal intensity of CDs-WO₃ was significantly higher than that of WO₃, indicating that more ·O₂^- and ·OH were produced. The analysis of bacterial cell activity demonstrated that CDs-WO₃ composites reduced the activity of respiratory chain dehydrogenase in bacteria, which resulted in increased lipid peroxidation in the cell membrane. The photocatalytic degradation performance showed that the photocatalytic degradation efficiency of CDs-WO₃ for methylene blue and malachite green reached 87 % and 88.04 %, respectively. The application of CDs-WO₃ in real water bodies also showed good antibacterial effect and dye degradation.

本研究将碳点（CD）和三氧化钨（WO3）结合起来，制备了 CDs-WO3 复合材料，用于光催化处理废水中的细菌和有机染料。同时还研究了其光学特性、抗菌特性、抗菌机理和光催化降解性能。CDs-WO3 复合材料的紫外吸收强度增强，可见光吸收范围扩大，电荷转移和载流子分离能力良好，带隙减小。CDs-WO3 对金黄色葡萄球菌的抗菌率可达 98%。研究发现 CDs-WO3 的抗菌机制是活性氧损伤机制。电子顺磁共振（EPR）分析表明，CDs-WO3 的活性氧信号强度明显高于 WO3，表明产生了更多的 -O2- 和 -OH。细菌细胞活性分析表明，CDs-WO3 复合材料降低了细菌呼吸链脱氢酶的活性，导致细胞膜脂质过氧化反应加剧。光催化降解性能表明，CDs-WO3 对亚甲基蓝和孔雀石绿的光催化降解效率分别达到 87 % 和 88.04 %。CDs-WO3 在实际水体中的应用也显示出良好的抗菌效果和染料降解效果。

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引用次数: 0

Research on the removal of fluoride from low-concentration fluorine-containing industrial wastewater using adsorption methods

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-02-10 DOI: 10.1016/j.bej.2025.109668

Benfu Luo , Yuhang Liu , Yujing Yan , Haixing He , Jie Yu , Qiang Chen

To address the need for deep fluoride removal of low-concentration fluorine-containing industrial wastewater (2–10 mg/L) to below 1.5 mg/L in centralized industrial wastewater treatment plants, a study was conducted using adsorption methods. The adsorption performance and influencing factors of four adsorbent materials—active alumina (AA), hydroxyapatite (HAP), fly ash (FA), and bone char (BC)—were investigated. The results indicate that positioning the adsorption fluoride removal filter at the tail end of the industrial wastewater treatment process yields a fluoride removal efficiency that is over 10 % higher compared to placing it at the front end. The investigation into the adsorption effectiveness of the four adsorbents revealed that HAP dosage only needs 2 g to reduce 250 ml fluorinated industrial wastewater containing 2–10 mg/L to the target concentration of 1.5 mg/L or less. Moreover, the adsorption process requires only 30 minutes. From the perspective of adsorption performance, HAP is the most effective, followed by AA. However, considering the cost of these adsorbents, AA is more suitable as an adsorbent for treating low-concentration fluorine-containing wastewater. The findings of this study can serve as a valuable reference for the process design of deep adsorption fluoride removal in projects involving fluorine-containing industrial wastewater.

{"title":"Research on the removal of fluoride from low-concentration fluorine-containing industrial wastewater using adsorption methods","authors":"Benfu Luo , Yuhang Liu , Yujing Yan , Haixing He , Jie Yu , Qiang Chen","doi":"10.1016/j.bej.2025.109668","DOIUrl":"10.1016/j.bej.2025.109668","url":null,"abstract":"<div><div>To address the need for deep fluoride removal of low-concentration fluorine-containing industrial wastewater (2–10 mg/L) to below 1.5 mg/L in centralized industrial wastewater treatment plants, a study was conducted using adsorption methods. The adsorption performance and influencing factors of four adsorbent materials—active alumina (AA), hydroxyapatite (HAP), fly ash (FA), and bone char (BC)—were investigated. The results indicate that positioning the adsorption fluoride removal filter at the tail end of the industrial wastewater treatment process yields a fluoride removal efficiency that is over 10 % higher compared to placing it at the front end. The investigation into the adsorption effectiveness of the four adsorbents revealed that HAP dosage only needs 2 g to reduce 250 ml fluorinated industrial wastewater containing 2–10 mg/L to the target concentration of 1.5 mg/L or less. Moreover, the adsorption process requires only 30 minutes. From the perspective of adsorption performance, HAP is the most effective, followed by AA. However, considering the cost of these adsorbents, AA is more suitable as an adsorbent for treating low-concentration fluorine-containing wastewater. The findings of this study can serve as a valuable reference for the process design of deep adsorption fluoride removal in projects involving fluorine-containing industrial wastewater.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"216 ","pages":"Article 109668"},"PeriodicalIF":3.7,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143395350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced degradation of petroleum hydrocarbons by immobilizing Acinetobacter

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-02-07 DOI: 10.1016/j.bej.2025.109666

Jienan Li , Hua Zhang , Kuixi Mei , Leni Sun , Li Wang , Changhai Liang

The highly efficient elimination of petroleum pollution is essential for addressing environmental issues and social sustainability. A hydrocarbon-degrading bacterium SHC is isolated from petroleum-contaminated soil in Chengdu, China, to augment bioremediation efforts against crude oil contamination. According to the analysis of 16S rDNA sequences, strain SHC was identified as Acinetobacter. The degradation rate of 2 g/L crude oil by free bacterium reached 30 % within 7 days. Through the analysis of hydrocarbon biodegradation, strain SHC was found to biodegrade more long-chain n-alkanes (C19–C29). Further, we delve into utilizing agar as a microbial immobilization matrix, supplemented with nutrient salts and oil-absorbing materials, to foster the growth and activity of the bacterium in challenging environments characterized by high salinity and acidity. In the final immobilization bacterium, the degradation rate of 3 % salt concentration reaches 22 %, the degradation rate of 5 g/L crude oil is 45 %, and the degradation rate at a pH of 10 is 74 %, and was 59 % more effective than the free bacterium on the removal of crude oil under the same conditions. These findings strongly indicate that encapsulating Acinetobacter SHC can protect them against extreme conditions, bolstering bioremediation endeavors.

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引用次数: 0

Advancements of the Vibrio coralliilyticus eDNA detection based on Co-Fe PBA-assisted biosensors for the rapid coral-disease warning

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-02-06 DOI: 10.1016/j.bej.2025.109662

Yibo Zhang , Hongjie Liu , Shaopeng Wang , Hao Fu , Yuanyu Xie , Chaoxin Zhang , Man Zhang , Jie Lu , Liwei Wang , Kefu Yu

Vibrio coralliilyticus (V. coralliilyticus) can lead to severe coral diseases and is one of the main causes of coral bleaching. However, there is still a lack of convenient, and non-invasive detection methods for V. coralliilyticus, which brings inconvenience to coral health management. Here, a portable electrochemical biosensor was developed based on environmental DNA (eDNA) and cobalt-iron Prussian Blue Analogue (Co-Fe PBA) electrode material for detecting V. coralliilyticus. Notably, this sensor identified and quantified V. coralliilyticus eDNA in water through specific DNA probes, overcoming the previously reported complex and destructive pretreatment processes. Additionally, thanks to the high specific surface area of Co-Fe PBA, which provided abundant sites for probe anchoring, the constructed portable biosensor exhibited excellent detection performances. Specifically, the detection limit was as low as 19.0 fM, and the linear range was from 100 fM to 100 nM. In subsequent coral infection experiments, the eDNA of V. coralliilyticus increased on the eve of coral bleaching. Based on this change, the occurrence of coral bleaching was successfully predicted. Therefore, this portable biosensor is suitable for dynamic monitoring of V. coralliilyticus, helping to reveal the potential risks to coral health and providing a reference for ecological decision-making for coral reef protection.

{"title":"Advancements of the Vibrio coralliilyticus eDNA detection based on Co-Fe PBA-assisted biosensors for the rapid coral-disease warning","authors":"Yibo Zhang , Hongjie Liu , Shaopeng Wang , Hao Fu , Yuanyu Xie , Chaoxin Zhang , Man Zhang , Jie Lu , Liwei Wang , Kefu Yu","doi":"10.1016/j.bej.2025.109662","DOIUrl":"10.1016/j.bej.2025.109662","url":null,"abstract":"<div><div><em>Vibrio coralliilyticus</em> (<em>V. coralliilyticus</em>) can lead to severe coral diseases and is one of the main causes of coral bleaching. However, there is still a lack of convenient, and non-invasive detection methods for <em>V. coralliilyticus</em>, which brings inconvenience to coral health management. Here, a portable electrochemical biosensor was developed based on environmental DNA (eDNA) and cobalt-iron Prussian Blue Analogue (Co-Fe PBA) electrode material for detecting <em>V. coralliilyticus</em>. Notably, this sensor identified and quantified <em>V. coralliilyticus</em> eDNA in water through specific DNA probes, overcoming the previously reported complex and destructive pretreatment processes. Additionally, thanks to the high specific surface area of Co-Fe PBA, which provided abundant sites for probe anchoring, the constructed portable biosensor exhibited excellent detection performances. Specifically, the detection limit was as low as 19.0 fM, and the linear range was from 100 fM to 100 nM. In subsequent coral infection experiments, the eDNA of <em>V. coralliilyticus</em> increased on the eve of coral bleaching. Based on this change, the occurrence of coral bleaching was successfully predicted. Therefore, this portable biosensor is suitable for dynamic monitoring of <em>V. coralliilyticus</em>, helping to reveal the potential risks to coral health and providing a reference for ecological decision-making for coral reef protection.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"216 ","pages":"Article 109662"},"PeriodicalIF":3.7,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143349956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing waste management and nutrient recovery: Preparation of N slow-release fertilizer using sewage sludge and its release behavior, effects on ryegrass (Lolium perenne L.) growth

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-02-06 DOI: 10.1016/j.bej.2025.109664

Diannan Huang , Yu Gao , Li Zhang , Rongxin Zhang , Yuhan Wu , Hao Guan , Di Luo

Efficient recovery and utilization of the nitrogen(N) contained in sewage sludge (SS) is a key consideration in the treatment of SS. In this study, a novel sludge hydrogel sphere slow-release N fertilizer was prepared based on the N-rich property of SS, using SS as a substrate and Fe³⁺(extracted from drinking water sludge (DWTS)), CMC, and ATP synergistically constructed as an encapsulated skeletal material. Based on the comprehensive consideration of hydrogel sphere formation performance, mechanical strength and encapsulation effect, the optimum mass ratio for the preparation of sludge hydrogel sphere was: M_SS: M_CMC: M_HATP: M_DWTS = 300: 4: 6:0.07; the concentration of HCl-modified ATP was 2.25 mol/L, and the number of coating layers was 1. The cumulative N release rates increased gradually with increasing soil moisture content, temperature and pH. The Korsmeyer-Peppas model can well describe the N release kinetic from sludge hydrogel spheres (R² = 0.9509). Potting experiments showed that the sludge hydrogel spheres not only promoted ryegrass growth, but also improved plant quality. The N retention mechanism of sludge hydrogel spheres mainly includes the physical barrier of coating and the adsorption of NH₄⁺ by electronegative electrostatic gravitational force of hydrogel spheres. And the degradation and rupture of the coating in the later stage guaranteed the N release. This study provides new ideas for the preparation of slow-release fertilizers from solid waste resource utilization.

{"title":"Enhancing waste management and nutrient recovery: Preparation of N slow-release fertilizer using sewage sludge and its release behavior, effects on ryegrass (Lolium perenne L.) growth","authors":"Diannan Huang , Yu Gao , Li Zhang , Rongxin Zhang , Yuhan Wu , Hao Guan , Di Luo","doi":"10.1016/j.bej.2025.109664","DOIUrl":"10.1016/j.bej.2025.109664","url":null,"abstract":"<div><div>Efficient recovery and utilization of the nitrogen(N) contained in sewage sludge (SS) is a key consideration in the treatment of SS. In this study, a novel sludge hydrogel sphere slow-release N fertilizer was prepared based on the N-rich property of SS, using SS as a substrate and Fe<sup>3+</sup>(extracted from drinking water sludge (DWTS)), CMC, and ATP synergistically constructed as an encapsulated skeletal material. Based on the comprehensive consideration of hydrogel sphere formation performance, mechanical strength and encapsulation effect, the optimum mass ratio for the preparation of sludge hydrogel sphere was: M<sub>SS</sub>: M<sub>CMC</sub>: M<sub>HATP</sub>: M<sub>DWTS</sub> = 300: 4: 6:0.07; the concentration of HCl-modified ATP was 2.25 mol/L, and the number of coating layers was 1. The cumulative N release rates increased gradually with increasing soil moisture content, temperature and pH. The Korsmeyer-Peppas model can well describe the N release kinetic from sludge hydrogel spheres (R<sup>2</sup> = 0.9509). Potting experiments showed that the sludge hydrogel spheres not only promoted ryegrass growth, but also improved plant quality. The N retention mechanism of sludge hydrogel spheres mainly includes the physical barrier of coating and the adsorption of NH<sub>4</sub><sup>+</sup> by electronegative electrostatic gravitational force of hydrogel spheres. And the degradation and rupture of the coating in the later stage guaranteed the N release. This study provides new ideas for the preparation of slow-release fertilizers from solid waste resource utilization.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"216 ","pages":"Article 109664"},"PeriodicalIF":3.7,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143349957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel aquaculture wastewater treatment by an efficient nitrogen-removing bacterium capable of simultaneous nitrification and denitrification

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-02-05 DOI: 10.1016/j.bej.2025.109663

Yuhang Song , Lingmin Zhao , Lixing Huang , Yingxue Qin , Jiaonan Zhang , Jiaoling Zhang , Qingpi Yan

Nitrogen pollution from aquaculture effluent poses a severe threat to aquatic ecosystems, necessitating efficient treatment solutions. In this study, a highly effective nitrogen-removing bacterium, designated SA20, was isolated from an aquaculture wastewater treatment system and identified as Paracoccus sp. Safety evaluations confirmed its non-hemolytic nature and broad antibiotic sensitivity, indicating its potential for safe application. Under optimized conditions (C/N ratio 16, 30 ℃, 140 rpm), the strain demonstrated complete removal of ammonium and nitrite, with over 95 % nitrate removal within 24 h. Nitrogen balance analysis revealed that approximately 70–80 % of total nitrogen was converted to gaseous form through denitrification, while 20–30 % was assimilated into biomass. When applied to actual aquaculture wastewater, SA20 achieved significant removal of various nitrogen forms, with total nitrogen removal exceeding 65 %. These findings demonstrate the potential of strain SA20 for practical application in aquaculture wastewater treatment, offering a promising biotechnology solution for sustainable aquaculture development.

{"title":"Novel aquaculture wastewater treatment by an efficient nitrogen-removing bacterium capable of simultaneous nitrification and denitrification","authors":"Yuhang Song , Lingmin Zhao , Lixing Huang , Yingxue Qin , Jiaonan Zhang , Jiaoling Zhang , Qingpi Yan","doi":"10.1016/j.bej.2025.109663","DOIUrl":"10.1016/j.bej.2025.109663","url":null,"abstract":"<div><div>Nitrogen pollution from aquaculture effluent poses a severe threat to aquatic ecosystems, necessitating efficient treatment solutions. In this study, a highly effective nitrogen-removing bacterium, designated SA20, was isolated from an aquaculture wastewater treatment system and identified as <em>Paracoccus</em> sp. Safety evaluations confirmed its non-hemolytic nature and broad antibiotic sensitivity, indicating its potential for safe application. Under optimized conditions (C/N ratio 16, 30 ℃, 140 rpm), the strain demonstrated complete removal of ammonium and nitrite, with over 95 % nitrate removal within 24 h. Nitrogen balance analysis revealed that approximately 70–80 % of total nitrogen was converted to gaseous form through denitrification, while 20–30 % was assimilated into biomass. When applied to actual aquaculture wastewater, SA20 achieved significant removal of various nitrogen forms, with total nitrogen removal exceeding 65 %. These findings demonstrate the potential of strain SA20 for practical application in aquaculture wastewater treatment, offering a promising biotechnology solution for sustainable aquaculture development.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"216 ","pages":"Article 109663"},"PeriodicalIF":3.7,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143335202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation of defective UiO-67 for CA immobilization to improve the ability of CO2 capture and conversion

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-01-31 DOI: 10.1016/j.bej.2025.109661

Rui Zhao, Wenhui Yang, Pengyan Yang, Yunhan Cao, Fenghuan Wang

Carbonic anhydrase (CA) has been suggested as a biocatalyst for carbon dioxide capture since it could effectively catalyze the hydration of carbon dioxide. Metal-organic frameworks (MOFs) have attracted significant attention in the development of immobilized enzyme carriers due to their pore tunability and excellent stability. In this study, UiO-67 materials with varying defect degrees (DUiO-67) were prepared using the organic linker missing method to enhance the catalytic performance. The results indicated that both the defect degree and the pore size of DUiO-67 materials decreased with an increase in acetic acid equivalent. After CA immobilization, all DUiO-67 materials exhibited excellent thermal, pH and recycling stability. CA@DUiO-67–25 showed higher enzyme catalytic activity and conversion ability than the other materials. After 10 cycles, CA@DUiO-67–25 retained 57 % of its initial conversion capacity. The construction of defective MOFs provides a promising new strategy for CA immobilization to achieve the catalytic conversion of CO₂.

{"title":"Preparation of defective UiO-67 for CA immobilization to improve the ability of CO2 capture and conversion","authors":"Rui Zhao, Wenhui Yang, Pengyan Yang, Yunhan Cao, Fenghuan Wang","doi":"10.1016/j.bej.2025.109661","DOIUrl":"10.1016/j.bej.2025.109661","url":null,"abstract":"<div><div>Carbonic anhydrase (CA) has been suggested as a biocatalyst for carbon dioxide capture since it could effectively catalyze the hydration of carbon dioxide. Metal-organic frameworks (MOFs) have attracted significant attention in the development of immobilized enzyme carriers due to their pore tunability and excellent stability. In this study, UiO-67 materials with varying defect degrees (DUiO-67) were prepared using the organic linker missing method to enhance the catalytic performance. The results indicated that both the defect degree and the pore size of DUiO-67 materials decreased with an increase in acetic acid equivalent. After CA immobilization, all DUiO-67 materials exhibited excellent thermal, pH and recycling stability. CA@DUiO-67–25 showed higher enzyme catalytic activity and conversion ability than the other materials. After 10 cycles, CA@DUiO-67–25 retained 57 % of its initial conversion capacity. The construction of defective MOFs provides a promising new strategy for CA immobilization to achieve the catalytic conversion of CO<sub>2</sub>.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"216 ","pages":"Article 109661"},"PeriodicalIF":3.7,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143168815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Highly selective detection of heavy metal ions in food and water using a 5-BHAHS@NC/MnO2-based electrochemical sensor

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-01-30 DOI: 10.1016/j.bej.2025.109660

Amany El Sikaily , Doaa Gh. Ghoniem , Omar Ramadan , Eslam M. El-Nahrery , Ahmed Shahat , Rabeay Y.A. Hassan

Human health and food safety face significant risks due to heavy metal contamination in food and water, as these metals can be accumulated to exhibit high toxicity even at low concentrations. Thus, electrochemical sensors, specifically voltammetry, provide a promising approach for simultaneous detection of heavy metal ions. In this study, a voltammetric assay was developed by directly immobilizing a newly synthesized ionophore structure, (E)-4-((5-bromo-2-hydroxybenzylidene) amino)-3-hydroxynaphthalene-1-sulfonic acid) (5-BHAHS), onto nano-cellulose (NC) synthesized via green methods, along with MnO₂ nanoparticles. The sensing material and its parent nano-cellulose base were characterized using HR-TEM, FT-IR, FE-SEM, XPS, and XRD. Subsequently, various factors influencing metal ion detection were studied and optimized, resulting in detection limits of 0.12 µM for both Hg²⁺ and Cd²⁺, and 0.03 µM for Pb²⁺. Optimal conditions for accumulation time, electrolyte pH, scan rate, selectivity, and sensitivity were determined to achieve rapid simultaneous detection at low concentrations. The 5-BHAHS@NC/MnO₂-based sensor proved to be a robust tool for the simultaneous detecting Hg²⁺, Cd²⁺, and Pb²⁺ ions in tap water, wastewater, and fish samples demonstrating high selectivity. Notably, the sensitivity achieved by the 5-BHAHS@NC/MnO₂ sensor is higher than that reported in previous studies, highlighting its superior performance, non-toxicity feedback and potential for practical applications in food safety and environmental monitoring.

{"title":"Highly selective detection of heavy metal ions in food and water using a 5-BHAHS@NC/MnO2-based electrochemical sensor","authors":"Amany El Sikaily , Doaa Gh. Ghoniem , Omar Ramadan , Eslam M. El-Nahrery , Ahmed Shahat , Rabeay Y.A. Hassan","doi":"10.1016/j.bej.2025.109660","DOIUrl":"10.1016/j.bej.2025.109660","url":null,"abstract":"<div><div>Human health and food safety face significant risks due to heavy metal contamination in food and water, as these metals can be accumulated to exhibit high toxicity even at low concentrations. Thus, electrochemical sensors, specifically voltammetry, provide a promising approach for simultaneous detection of heavy metal ions. In this study, a voltammetric assay was developed by directly immobilizing a newly synthesized ionophore structure, (<em>E</em>)-4-((5-bromo-2-hydroxybenzylidene) amino)-3-hydroxynaphthalene-1-sulfonic acid) (5-BHAHS), onto nano-cellulose (NC) synthesized via green methods, along with MnO<sub>2</sub> nanoparticles. The sensing material and its parent nano-cellulose base were characterized using HR-TEM, FT-IR, FE-SEM, XPS, and XRD. Subsequently, various factors influencing metal ion detection were studied and optimized, resulting in detection limits of 0.12 µM for both Hg²⁺ and Cd²⁺, and 0.03 µM for Pb²⁺. Optimal conditions for accumulation time, electrolyte pH, scan rate, selectivity, and sensitivity were determined to achieve rapid simultaneous detection at low concentrations. The 5-BHAHS@NC/MnO<sub>2</sub>-based sensor proved to be a robust tool for the simultaneous detecting Hg²⁺, Cd²⁺, and Pb²⁺ ions in tap water, wastewater, and fish samples demonstrating high selectivity. Notably, the sensitivity achieved by the 5-BHAHS@NC/MnO₂ sensor is higher than that reported in previous studies, highlighting its superior performance, non-toxicity feedback and potential for practical applications in food safety and environmental monitoring.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"216 ","pages":"Article 109660"},"PeriodicalIF":3.7,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143168816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

One-step solvothermal preparation of Fe3O4@HA magnetic nanomicrospheres for simultaneous removal of tetracycline antibiotics

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-01-29 DOI: 10.1016/j.bej.2025.109651

Sisi Li , Shuang Li , Xiang Li , Xiaodan Zeng , Jie Chen , Zhigang Liu , Shihua Yu , Junjing Hao

Humic acid (HA) is a naturally biomass, that is considered as an ideal adsorbent material due to the abundance of functional groups on its surface. For this purpose, the highly dispersible magnetic humic acid (Fe₃O₄@HA) has been prepared by one-step solvothermal method. The developed Fe₃O₄@HA not only has a strong separation, but also can simultaneously adsorb tetracycline antibiotics (tetracycline, chlortetracycline hydrochloride and doxycycline hydrochloride). Batch sorption experiments convinced that the maximum tetracycline antibiotics adsorption capacity of Fe₃O₄@HA is 45.58, 86.87, and 88.46 mg/g and the largest removal of 94.5 %, 96.3 %, and 97.4 %, fast equilibrium time of 60 min. Meanwhile, the adsorption process is agreement with pseudo-second-order adsorption kinetics and Langmuir adsorption isotherm model. Thermodynamic data indicate that this adsorption is endothermic and spontaneous. Moreover, the designed Fe₃O₄@HA with the characteristic of anti-interference capabilities, a variety of water-matrix suitability and maintaining at least 80 % tetracycline antibiotics removal after five adsorption cycles. Finally, the adsorption mechanisms are explored by XPS and FT-IR analysis, which show that mainly involve electrostatic adsorption; hydrogen bonding, π–π, oxygen-containing functional group interactions; and physical adsorption on the surface. In conclusion, Fe₃O₄@HA demonstrates considerable promise as an adsorbent for treating antibiotic-contaminated water.

{"title":"One-step solvothermal preparation of Fe3O4@HA magnetic nanomicrospheres for simultaneous removal of tetracycline antibiotics","authors":"Sisi Li , Shuang Li , Xiang Li , Xiaodan Zeng , Jie Chen , Zhigang Liu , Shihua Yu , Junjing Hao","doi":"10.1016/j.bej.2025.109651","DOIUrl":"10.1016/j.bej.2025.109651","url":null,"abstract":"<div><div>Humic acid (HA) is a naturally biomass, that is considered as an ideal adsorbent material due to the abundance of functional groups on its surface. For this purpose, the highly dispersible magnetic humic acid (Fe<sub>3</sub>O<sub>4</sub>@HA) has been prepared by one-step solvothermal method. The developed Fe<sub>3</sub>O<sub>4</sub>@HA not only has a strong separation, but also can simultaneously adsorb tetracycline antibiotics (tetracycline, chlortetracycline hydrochloride and doxycycline hydrochloride). Batch sorption experiments convinced that the maximum tetracycline antibiotics adsorption capacity of Fe<sub>3</sub>O<sub>4</sub>@HA is 45.58, 86.87, and 88.46 mg/g and the largest removal of 94.5 %, 96.3 %, and 97.4 %, fast equilibrium time of 60 min. Meanwhile, the adsorption process is agreement with pseudo-second-order adsorption kinetics and Langmuir adsorption isotherm model. Thermodynamic data indicate that this adsorption is endothermic and spontaneous. Moreover, the designed Fe<sub>3</sub>O<sub>4</sub>@HA with the characteristic of anti-interference capabilities, a variety of water-matrix suitability and maintaining at least 80 % tetracycline antibiotics removal after five adsorption cycles. Finally, the adsorption mechanisms are explored by XPS and FT-IR analysis, which show that mainly involve electrostatic adsorption; hydrogen bonding, π–π, oxygen-containing functional group interactions; and physical adsorption on the surface. In conclusion, Fe<sub>3</sub>O<sub>4</sub>@HA demonstrates considerable promise as an adsorbent for treating antibiotic-contaminated water.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"216 ","pages":"Article 109651"},"PeriodicalIF":3.7,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143335201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0