Biochemical Engineering Journal最新文献_第2页

Transient heat and mass transfer in a wall-cooled packed-tray bioreactor for solid-state fermentation: An engineering framework for parameter estimation 固态发酵用壁冷填料托盘生物反应器的瞬态传热传质：参数估计的工程框架

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-01-03 DOI: 10.1016/j.bej.2026.110071

Gerardo A. Gómez-Ramos , Lilia A. Prado-Barragán , Sergio Huerta-Ochoa , Victor M. Rivera , Ángeles García-Hernández , Carlos O. Castillo-Araiza

Solid-state fermentation (SSF) of agrifood byproducts (AFBP) offers a sustainable route for producing high-value biomolecules, yet its industrial adoption is constrained by limited understanding of transport phenomena in wall-cooled packed bioreactors. Despite their potential for large-scale SSF, reliable methods for describing heat and mass transfer remain scarce. Building on previous fluid-dynamic studies, this work characterizes intrinsic transport mechanisms in a bench-scale wall-cooled tray packed with AFBP as substrate. An engineering framework not previously applied to these systems, integrating abiotic experiments, fluid dynamics, and transient analysis, was applied to estimate the effective thermal conductivity (

k_{eff}

), wall heat transfer coefficient (

h_{w}

), and axial dispersion coefficient (

D_{ax}

). Experiments under conditions favorable to Yarrowia lipolytica 2.2ab (318.15 K, 60 % moisture on a dry-weight basis, and inlet specific airflow rates, VkgM, of 1.875–6.250 L.kg⁻¹.min⁻¹) yielded values of 0.565–4.34 W.m⁻¹ .K⁻¹ for

k_{eff}

, 0.865–25.9 W.m⁻².K⁻¹ for

h_{w}

, and 2.60 × 10⁻³ –5.30 × 10⁻³ m².s⁻¹ for

D_{ax}

. These results differ from previous SSF studies employing AFBP, highlighting the influence of reactor configuration, operating conditions, and parameter estimation approach. The proposed methodology provides a reliable framework for evaluating heat and mass transfer in SSF systems using AFBP as a substrate and establishes a foundation for predictive design and scale-up of packed bioreactors in biotechnological applications.

农业食品副产品（AFBP）的固态发酵（SSF）为生产高价值生物分子提供了一条可持续的途径，但其工业应用受到对壁冷填充生物反应器中运输现象的有限理解的限制。尽管它们具有大规模SSF的潜力，但描述传热和传质的可靠方法仍然很少。在先前的流体动力学研究的基础上，本研究表征了以AFBP为衬底的台式壁冷托盘中的固有输运机制。一个以前没有应用于这些系统的工程框架，整合了非生物实验、流体动力学和瞬态分析，用于估计有效导热系数（keff）、壁面传热系数（hw）和轴向分散系数（Dax）。实验条件为脂肪耶氏菌2.2ab (318.15 K)， 60%的水分（干重基础），入口比气流速率（VkgM）为1.875-6.250 L.kg⁻¹。w - m（⁻）的值为0.565-4.34。K⁻¹for keff, 0.865-25.9 w m⁻²。K⁻¹，以及2.60 × 10⁻³-5.30 × 10⁻³²。（对Dax来说）这些结果与先前使用AFBP的SSF研究不同，突出了反应器配置、操作条件和参数估计方法的影响。所提出的方法为评估使用AFBP作为基质的SSF系统中的传热传质提供了可靠的框架，并为生物技术应用中填料生物反应器的预测设计和放大奠定了基础。

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引用次数: 0

Sustainable chitosan-egg white protein nanofiber membranes for efficient bacterial capture and killing 可持续的壳聚糖-蛋清蛋白纳米纤维膜，用于有效的细菌捕获和杀死

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-01-02 DOI: 10.1016/j.bej.2025.110067

Thi Tam An Tran , Edouard Gnoumou , Quang-Vinh Le , Nguyen The Duc Hanh , Nanthiya Hansupalak , Yi-Hua Gao , Bing-Lan Liu , Chi-Yun Wang , Kuei-Hsiang Chen , Yu-Kaung Chang

Egg whites are an abundant source of natural proteins with inherent biological activities, including antibacterial properties. In this study, a novel antibacterial nanofiber membrane was developed by immobilizing egg white proteins (EWP) onto an electrospun polyacrylonitrile (PAN) membrane. The PAN membrane was first modified via alkaline hydrolysis to introduce carboxyl groups (P-COOH), followed by chitosan (CS) grafting (P-COOH-CS) to enhance biocompatibility and protein attachment. EWP was then immobilized onto the surface through ionic and covalent interactions. The immobilization process was optimized by varying pH, CS molecular weight, and solute concentration. The resulting P-COOH-CS-EWP membrane exhibited strong antibacterial efficacy, achieving 98.25 ± 2.09 % reduction in Gram-negative E. coli and 88.24 ± 2.93 % reduction in Gram-positive S. aureus. Furthermore, the nanofiber membrane demonstrated good reusability and stability, retaining 75.84 ± 3.73 % activity after three cycles and 76.41 ± 0.79 % after four weeks of storage. This work highlights the potential of EWP-functionalized nanofiber membranes as a cost-effective, eco-friendly antibacterial material. The approach provides a sustainable strategy for recycling EWP waste and can be applied in biomedical, food packaging, and environmental applications that require antibacterial protection.

蛋清是天然蛋白质的丰富来源，具有固有的生物活性，包括抗菌特性。本研究将蛋清蛋白（EWP）固定在静电纺聚丙烯腈（PAN）膜上，制备了一种新型抗菌纳米纤维膜。首先对PAN膜进行碱性水解修饰，引入羧基（P-COOH），然后进行壳聚糖（CS）接枝（P-COOH-CS）以增强生物相容性和蛋白质附着。然后通过离子和共价相互作用将EWP固定在表面。通过改变pH、CS分子量和溶质浓度对固定化工艺进行优化。所得P-COOH-CS-EWP膜具有较强的抗菌效果，对革兰氏阴性大肠杆菌和革兰氏阳性金黄色葡萄球菌的抗菌效果分别达到98.25 ± 2.09 %和88.24 ± 2.93 %。此外，纳米纤维膜表现出良好的可重复使用性和稳定性，三次循环后活性保持75.84 ± 3.73 %，四周后活性保持76.41 ± 0.79 %。这项工作强调了ewp功能化纳米纤维膜作为一种具有成本效益，环保的抗菌材料的潜力。该方法为EWP废物的回收提供了一种可持续的策略，可以应用于需要抗菌保护的生物医学，食品包装和环境应用。

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引用次数: 0

Coupled biodegradable polymer composite and sulfur-driven mixotrophic denitrification toward municipal tailwater polishing: Process performance and microbial synergism 耦合可生物降解聚合物复合材料和硫驱动混合营养化反硝化用于城市尾水抛光：工艺性能和微生物协同作用

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-01-02 DOI: 10.1016/j.bej.2025.110070

Qian-Dong Qin , Jun-Cheng Han , Jin Cai , Tong Cai , Hao-Nan Xiao , Hou-Yun Yang , Kan Wang

To address the limited denitrification efficiency caused by low carbon-to-nitrogen (C/N) ratio in municipal wastewater effluents, a mixotrophic denitrification reactor (PWSMDR) was constructed utilizing polycaprolactone/wheat straw composite (PWS) and sulfur as dual electron donors. Its performance was systematically compared with a heterotrophic denitrification reactor (PWHDR) employing PWS as the sole electron donor. The results demonstrated that PWSMDR achieved a high nitrate removal efficiency of approximately 99.2 % under a hydraulic retention time (HRT) of 2 h, with a nitrate removal rate of 0.38 kg N/m³/d, representing an increase of nearly 23 % compared with PWHDR. Furthermore, PWSMDR exhibited enhanced resilience to shock loading. Autotrophic and heterotrophic denitrification pathways in PWSMDR were responsible for 33.6–62.3 % and 37.7–66.4 % of the nitrate removal, respectively. High-throughput sequencing further revealed a significant enrichment of key autotrophic denitrifiers (e.g. Thiobacillus and Sulfurimonas) and heterotrophic denitrifiers (e.g. Thauera, Dechloromonas, and Diaphorobacter) in PWSMDR. The abundance of key functional genes involved in carbon, sulfur, and nitrogen transformations was enhanced in PWSMDR, promoting more efficient nitrate reduction to N₂. Additionally, the effluent COD and TN in PWSMDR were maintained at approximately 14 mg/L and 0.95 mg/L, respectively. These findings demonstrate that the PWS–sulfur mixotrophic denitrification strategy not only ensures highly efficient and stable nitrogen removal, but also offers a cost-effective and sustainable engineering approach for municipal tailwater polishing.

为解决城市污水中低碳氮比（C/N）导致反硝化效率受限的问题，以聚己内酯/麦秸复合材料（PWS）和硫为双电子供体，构建了混合营养化反硝化反应器（PWSMDR）。将其性能与PWS作为唯一电子给体的异养反硝化反应器（PWHDR）进行了系统比较。结果表明，在水力停留时间（HRT）为2 h的条件下，PWSMDR的硝酸盐去除率约为99.2% %，硝酸盐去除率为0.38 kg N/m3/d，比PWHDR提高了近23% %。此外，PWSMDR对冲击载荷表现出更强的弹性。在PWSMDR中，自养和异养反硝化途径的硝酸盐去除率分别为33.6-62.3 %和37.7-66.4 %。高通量测序进一步揭示了PWSMDR中关键自养反硝化菌（如硫杆菌和硫单胞菌）和异养反硝化菌（如Thauera、decchloromonas和Diaphorobacter）的显著富集。在PWSMDR中，参与碳、硫和氮转化的关键功能基因的丰度得到了增强，促进了硝酸盐更有效地还原为N2。此外，PWSMDR出水COD和TN分别维持在约14 mg/L和0.95 mg/L。这些结果表明，pws -硫混合营养化反硝化策略不仅保证了高效稳定的脱氮，而且为城市尾水净化提供了一种经济、可持续的工程方法。

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引用次数: 0

Nanobody-drug conjugates as versatile tools for improving therapeutic potential 纳米-药物缀合物是提高治疗潜力的通用工具

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-01-02 DOI: 10.1016/j.bej.2026.110072

Sneha Banerjee, Anna Mariya, Sreeja Vangapally, Bhaskar Paidimuddala

Nanobody-Drug Conjugates (NDCs) represent a rapidly emerging class of targeted therapeutics that merge the precision of nanobodies with the potency of cytotoxic or functional drug payloads. Unlike traditional Antibody-Drug Conjugates (ADCs), NDCs offer superior advantages, such as improved tissue penetration, faster systemic clearance, and compatibility with modular engineering platforms. Despite these promising features, NDCs remain understated in clinical pipelines, emphasizing the need for integrated insights into their therapeutic development. This review provides an inclusive analysis of structural and functional optimization strategies for NDCs, including nanobody selection, site-specific conjugation chemistries, linker design, and payload engineering to enhance intracellular delivery and therapeutic index. This review also highlights unresolved challenges, including maintaining the biochemical stability of linkers under physiological conditions, the structural and functional integration of diverse drug payloads with nanobody scaffolds, and limited mechanistic insights into nanobody pharmacodynamics and fate following intracellular drug release. Furthermore, this review discusses the recent advancements in both preclinical models and early clinical investigations, with a focus on the expanding therapeutic utility of NDCs in oncology, infectious diseases, and molecular imaging applications to accelerate the clinical viability of NDCs as next-generation biologics.

纳米体-药物偶联物（ndc）是一种迅速兴起的靶向治疗方法，它将纳米体的精确性与细胞毒性或功能性药物有效载荷的效力结合在一起。与传统的抗体-药物偶联物（adc）不同，ndc具有优越的优势，例如改善组织穿透，更快的系统清除以及与模块化工程平台的兼容性。尽管有这些有希望的特性，ndc在临床管道中仍然被低估，强调需要对其治疗开发进行综合见解。本文综述了ndc的结构和功能优化策略，包括纳米体选择、位点特异性偶联化学、连接体设计和有效载荷工程，以提高细胞内递送和治疗指数。这篇综述还强调了尚未解决的挑战，包括在生理条件下保持连接体的生化稳定性，不同药物有效载荷与纳米体支架的结构和功能整合，以及纳米体药效学和细胞内药物释放后命运的有限机制见解。此外，本文还讨论了临床前模型和早期临床研究的最新进展，重点讨论了ndc在肿瘤学、感染性疾病和分子成像应用中的治疗应用，以加速ndc作为下一代生物制剂的临床可行性。

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引用次数: 0

Optimum biohydrogen production by combination of fermentation and microbial electrolysis cell: Bioreactor design, scale-up, and technology readiness level analysis 结合发酵和微生物电解细胞的最佳生物制氢：生物反应器设计、放大和技术准备水平分析

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-12-31 DOI: 10.1016/j.bej.2025.110066

Vikash, Nitai Basak

The integration of fermentation with microbial electrolysis cells (MECs) presents a novel approach to enhance biohydrogen production from organic residues, thereby overcoming the limitations of traditional fermentation systems. This review critically assesses the current state of this combined technology, focusing on bioreactor engineering, scale-up challenges, and its readiness for commercial application. The fermentation stage effectively converts complex biomass into a volatile fatty acid (VFA) rich effluent, which serves as an ideal substrate for the subsequent MEC to achieve near-complete substrate valorisation and significantly higher hydrogen yields. Analysis of bioreactor design reveals a clear trend toward reducing capital costs through the development of low-cost, non-precious metal cathode catalysts, such as nickel alloys and stainless steel, to replace platinum, as well as the adoption of simplified, membraneless reactor architectures. The comprehensive Technology Readiness Level (TRL) assessment places the system at TRL 3–4. However, scaling this technology from the laboratory to industrial application presents considerable challenges. We conclude that bridging the gap to pilot-scale (TRL 6) requires overcoming specific engineering hurdles such as maintaining high current densities in large-scale reactors, mitigating biofouling, and optimizing various biological and physical factors affecting biohydrogen yield in fermentation and MEC systems.

将发酵与微生物电解细胞（MECs）相结合，为提高有机残留物产氢提供了一种新的途径，从而克服了传统发酵系统的局限性。这篇综述批判性地评估了这种组合技术的现状，重点是生物反应器工程、扩大规模的挑战以及其商业应用的准备情况。发酵阶段有效地将复杂的生物质转化为挥发性脂肪酸（VFA）丰富的排出物，作为后续MEC的理想底物，以实现近乎完全的底物增值和显着提高的氢气产量。对生物反应器设计的分析揭示了一个明显的趋势，即通过开发低成本、非贵金属阴极催化剂（如镍合金和不锈钢）来取代铂，以及采用简化的无膜反应器结构来降低资本成本。综合技术准备水平（TRL）评估将该系统置于TRL 3-4。然而，将这项技术从实验室扩展到工业应用存在相当大的挑战。我们得出的结论是，弥合中试规模（TRL 6）的差距需要克服特定的工程障碍，例如在大型反应器中保持高电流密度，减轻生物污染，以及优化影响发酵和MEC系统中生物氢产量的各种生物和物理因素。

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引用次数: 0

Enhancing dry anaerobic digestion of pig manure via in-situ ammonia capture by adding magnesium salt-pretreated wheat straw 添加镁盐预处理麦秸原位捕氨强化猪粪干式厌氧消化

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-12-31 DOI: 10.1016/j.bej.2025.110064

Hongyuan Sun , Jiaqi Liu , Xuewen Gao , Kuizu Su , Rui Tang , Xinmin Zhan , Zhen-Hu Hu

Dry anaerobic digestion (AD) is a promising technology for pig manure treatment, yet it is challenged by ammonia inhibition. Magnesium salt addition promotes the formation of magnesium ammonium phosphate (MAP), which in-situ captures ammonia nitrogen and mitigates inhibition, while high solids content limits MAP crystallization. This study evaluated the effect of magnesium salt pretreatment of wheat straw on dry AD of pig manure. Four Mg:P molar ratios (1.0:1.0, 1.5:1.0, 2.0:1.0, and 3.0:1.0) were investigated. Adding magnesium salt-pretreated wheat straw under molar ratios of 1.0:1.0 and 1.5:1.0 captured 14.3–14.4 % of ammonia nitrogen and reduced free ammonia by 19.0–19.1 %. Methane production increased by 15.0–19.2 % and substrate degradation by 8.4–8.6 %. Such pretreatment promoted MAP nucleation and growth on the straw surface, facilitating ammonia nitrogen capture. The capture of ammonia nitrogen restored the abundance of acetoclastic methanogens from 7.3 % to 12.5 %, thereby enhancing methane production. These results provide a practical strategy for mitigating ammonia inhibition in dry AD of pig manure.

干式厌氧消化（AD）是一种很有前途的猪粪处理技术，但它受到氨抑制的挑战。镁盐的加入促进了磷酸铵镁（MAP）的形成，从而原位捕获氨氮，减轻了抑制作用，而高固体含量限制了MAP的结晶。研究了麦秸镁盐预处理对猪粪干AD的影响。研究了4种Mg:P摩尔比（1.0:1.0、1.5:1.0、2.0:1.0和3.0:1.0）。添加量比为1.0:1.0和1.5:1.0的镁盐预处理麦秸，可捕获14.3 ~ 14.4 %的氨氮，减少19.0 ~ 19.1 %的游离氨。甲烷产量提高15.0 ~ 19.2 %，底物降解提高8.4 ~ 8.6 %。这种预处理促进了秸秆表面MAP的成核和生长，有利于氨氮的捕获。氨氮的捕获使丙酮裂解产甲烷菌的丰度从7.3 %恢复到12.5 %，从而提高了甲烷产量。这些结果为减轻猪粪干AD中的氨抑制作用提供了可行的策略。

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引用次数: 0

Purple-red azaphilone alkaloids from Talaromyces amestolkiae showing antimicrobial activity 具有抗菌活性的紫红色氮蚜酮生物碱

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-12-30 DOI: 10.1016/j.bej.2025.110065

Fernanda de Oliveira , Caio de Azevedo Lima , Diana Cláudia Gouveia Alves Pinto , Laura Carmona Ferreira , Tais Maria Bauab , Silvio Silvério da Silva , Valéria Carvalho Santos-Ebinuma

The global rise of antimicrobial resistance highlights the urgent need for alternative bioactive compounds from sustainable sources. Fungal colorants, especially azaphilones, represent promising natural molecules due to their structural diversity, colorant properties, and biological activities. Talaromyces amestolkiae is a well-known source of Monascus-like azaphilone colorants free of mycotoxins, yet its antimicrobial potential and production dynamics remain underexplored. In this study, submerged cultivations were conducted to improve colorant production, isolate novel compounds, and assess antimicrobial activity. The effects of glucose concentration and hydrodynamic conditions, expressed through agitation, aeration, and the volumetric oxygen transfer coefficient (k_La), were investigated. The optimal condition (100 rpm, 1.0 vvm, 10 g·L⁻¹ of glucose) promoted balanced oxygen availability and carbon utilization, resulting in maximum colorant yield. Two novel purple-red azaphilone alkaloids, cis-amestolkin (1) and trans-amestolkin (2), were isolated and characterized by UV–Vis, LC-HRMS, and NMR spectroscopy. These compounds are geometric isomers of nitrogen-containing azaphilones featuring a γ-lactone ring and a unique 4-amino-6-hydroxyhexanoic acid moiety. Antimicrobial assays revealed that T. amestolkiae-derived metabolites exhibit pronounced inhibitory activity against Pseudomonas aeruginosa, Staphylococcus aureus, and Salmonella setubal, highlighting their bioactive potential . This work integrates bioprocess improvement with metabolite identification and biological evaluation, underscoring the value of fungal colorants as multifunctional, sustainable agents for industrial and pharmaceutical applications.

全球抗菌素耐药性的上升凸显了从可持续来源寻找替代生物活性化合物的迫切需要。真菌着色剂，特别是氮唑啉，由于其结构多样性、着色剂特性和生物活性，是一种很有前途的天然分子。amestolkiae Talaromyces amestolkiae是一种众所周知的不含霉菌毒素的红曲霉样氮蚜酮着色剂的来源，但其抗菌潜力和生产动态仍未得到充分开发。在本研究中，通过潜水培养来提高着色剂的产量，分离新的化合物，并评估抗菌活性。考察了葡萄糖浓度和流体动力条件对搅拌、曝气和体积氧传递系数（kLa）的影响。最佳条件（100 rpm, 1.0 vvm， 10 g·L⁻¹葡萄糖）促进了氧可用性和碳利用的平衡，从而获得了最大的着色剂收率。分离得到了两种新的紫红色氮蚜酮生物碱顺式-阿梅斯托金(1)和反式阿梅斯托金(2)，并通过UV-Vis、LC-HRMS和NMR对其进行了表征。这些化合物是含氮氮氮酮的几何异构体，具有γ-内酯环和独特的4-氨基-6-羟基己酸部分。抗菌实验显示，金黄色葡萄球菌衍生的代谢物对铜绿假单胞菌、金黄色葡萄球菌和settubal沙门氏菌具有明显的抑制活性，突出了其生物活性潜力。这项工作将生物过程改进与代谢物鉴定和生物学评价相结合，强调了真菌着色剂作为多功能、可持续的工业和制药应用剂的价值。

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引用次数: 0

Model-based optimization of stripping onset in Saccharomyces cerevisiae very-high-gravity ethanol fermentations 基于模型的酿酒酵母极重乙醇发酵脱皮起始优化

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-12-27 DOI: 10.1016/j.bej.2025.110057

I.I.K. Veloso , V.T. Mazziero , D.A. Lemos , A.J.G. Cruz , M.O. Cerri , A.C. Badino

Very-high-gravity (VHG) fermentation increases ethanol yield and decreases water use, but it imposes severe osmotic and ethanolic stress on Saccharomyces cerevisiae, which frequently limits overall productivity. In situ ethanol removal through CO₂ gas stripping can mitigate this inhibition. However, the optimal timing for stripping onset under different temperature conditions remains not fully characterized. This study examined the effect of stripping initiation timing in VHG fed-batch fermentations at 28, 30, 32, and 34°C. A mechanistic fermentation model based on Monod-type kinetics with ethanol inhibition terms and coupled gas-liquid mass transfer was estimated using differential evolution. Distinct, temperature-specific ethanol concentration thresholds for stripping onset were identified, which maximized ethanol productivity (

P_{CE}

). Plateau analysis, based on identification of concentration regions where delayed initiation produced negligible changes in

P_{CE}

, refined these thresholds to within ±0.5 % of the maximum productivity. This resulted in CO₂ gas savings equivalent to 1.05 ± 0.06 h per fermentation cycle. Model-based time-varying temperature control optimization predicted an increase in ethanol productivity to 12.32 g L⁻¹ h⁻¹. The findings provide a simulation and parameter estimation framework for temperature-integrated extractive control strategies to improve ethanol production in high-biomass VHG fermentations.

非常高重力（VHG）发酵提高了乙醇产量，减少了水的使用，但它对酿酒酵母施加了严重的渗透和乙醇压力，这往往限制了总体生产力。通过CO2气提原位去除乙醇可以减轻这种抑制作用。然而，在不同温度条件下，汽提开始的最佳时机尚未完全确定。本研究考察了在28、30、32和34℃条件下VHG补料分批发酵中剥离起始时间的影响。利用微分演化方法估计了一个基于monod型动力学、乙醇抑制项和气液耦合传质的机制发酵模型。确定了不同的温度特异性乙醇浓度阈值，以最大化乙醇生产率（PCE）。平台分析，基于识别延迟起始产生可忽略不计的PCE变化的浓度区域，将这些阈值细化到最大生产力的±0.5%以内。这导致二氧化碳气体节省相当于1.05±0.06小时每个发酵周期。基于模型的时变温度控制优化预测乙醇产量将增加到12.32 g L - 1 h - 1。研究结果为温度集成萃取控制策略提供了模拟和参数估计框架，以提高高生物量VHG发酵的乙醇产量。

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引用次数: 0

Iron-carbon enhanced CW-MFC for treating high COD/TN rural greywater: Performance and microbial synergy in bioenergy recovery 铁碳强化CW-MFC处理高COD/TN乡村灰水：生物能源回收中的性能和微生物协同作用

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-12-22 DOI: 10.1016/j.bej.2025.110054

Zhiwei Zhu , Yahui Li , Junyong He , Yulian Li , Peidong Hong , Chao Xie , Zijian Wu , Jiandong Lu , Dandan Yang , Lingtao Kong

This study developed an iron-carbon enhanced constructed wetland-microbial fuel cell (ICCW-MFC) to treat real rural greywater with high COD/TN ratios (13.1–16.0). The ICCW-MFC significantly improved pollutant removal, especially under extended hydraulic retention time (HRT), increasing removal efficiencies by 19.4 % (COD), 16.5 % (TN), 20.7 % (NH₄⁺-N), and 13.6 % (TP). The ICCW-MFC consistently produced higher quality effluent, with average reductions of 5.1 % (COD), 4.5 % (TN), 7.3 % (NH₄⁺-N), and 5.5 % (TP) compared to the control. Bioenergy recovery was substantially boosted, with a 2.8-fold higher current density and an 8-fold greater maximum power density compared to a conventional CW-MFC (lab control). Microbial diversity increased significantly (P < 0.001) at the anode, enriching electroactive genera (e.g., Geobacter) and forming a synergistic degradation network. PCA identified HRT as the dominant operational factor, while 3D-EEM confirmed effective degradation of tryptophan-like organics. The ICCW-M demonstrates promise for decentralized greywater treatment with concurrent energy recovery.

本研究开发了一种铁碳强化人工湿地-微生物燃料电池（ICCW-MFC），用于处理COD/TN比值（13.1 ~ 16.0）较高的农村真实灰水。ICCW-MFC显著提高了污染物的去除率，特别是在延长水力停留时间（HRT）下，其去除率分别提高了19.4 % （COD）、16.5 % （TN）、20.7 % （NH₄+ -N）和13.6 % （TP）。ICCW-MFC持续产生更高质量的出水，与对照组相比，平均降低了5.1 % （COD）、4.5 % （TN）、7.3 % （NH₄+ -N）和5.5 % （TP）。生物能源回收率大大提高，与传统的CW-MFC（实验室对照）相比，电流密度提高2.8倍，最大功率密度提高8倍。阳极处微生物多样性显著增加（P <； 0.001），使电活性菌（如Geobacter）富集，形成协同降解网络。PCA确定HRT是主要的操作因素，而3D-EEM证实了色氨酸样有机物的有效降解。ICCW-M展示了分散式污水处理和同步能量回收的前景。

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引用次数: 0

Bioconversion kinetics and valorisation of oil palm empty fruit bunches for biomethane production and lignin derived compounds 油棕空果束用于生物甲烷生产和木质素衍生化合物的生物转化动力学和价值评价

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-12-20 DOI: 10.1016/j.bej.2025.110053

Irnia Nurika , Eka Nur Shabrina , Nurul Azizah , Sri Suhartini , Guy C. Barker , Timothy D.H. Bugg

This study assesses the facultative anaerobe Comamonas testosteroni as a mild, chemical-free pre-treatment for valorising oil palm empty fruit bunches (OPEFB) into biomethane and lignin-derived aromatics. Incubation with 2 % (v/v) C. testosteroni for 7 days at 30 °C, reduced lignin to 15.67 % (33.42 % removal), while retaining high cellulose (53.48–56.19 %) and hemicellulose (up to 16.21 %). Pre-treated OPEFB showed 20 % weight loss (vs 8 % in controls) and a 51 % rise in total soluble phenols, evidencing active lignin depolymerisation. GC–MS of liquor and solids qualitatively confirmed lignin breakdown and identified representative low-molecular-weight products (e.g., phenol, betulin, acetic acid and benzoxazole), supporting co-product potential. In biochemical methane potential tests, the pre-treated residue achieved a specific methane potential of 0.173 m³ CH₄ kg⁻¹ VS, an 85 % increase over the abiotic residue and higher than non-treated OPEFB. Kinetic fitting with Transference, Modified Gompertz and Logistic models yielded good agreement (R² up to 0.92), with Modified Gompertz best capturing cumulative methane production and the shortest lag phases. A simple energy check indicates a favourable margin: the incremental methane (ΔSMP ≈ 0.08 m³ CH₄ kg⁻¹ VS) equates ∼0.8 kWh kg⁻¹ VS, whereas estimated mixing for pre-treatment is ∼0.017–0.034 kWh kg⁻¹ VS. Overall C. testosteroni pre-treatment enables dual valorisation (biomethane plus aromatics) under mesophilic conditions, offering a practical route for integrated OPEFB biorefineries.

本研究评估了兼性厌氧菌睾酮单胞菌作为一种温和的、无化学物质的预处理，用于将油棕空果束（OPEFB）转化为生物甲烷和木质素衍生的芳烃。与2 % (v/v) C. steroni在30°C下孵育7天，木质素降至15.67 %（去除率33.42 %），同时保留高纤维素（53.48-56.19 %）和半纤维素（高达16.21 %）。预处理后的OPEFB重量减轻了20% %（对照组为8% %），可溶性酚总含量增加了51% %，证明木质素解聚活性。液相和固相的GC-MS定性证实了木质素的分解，并鉴定了具有代表性的低分子量产物（如苯酚、白桦脂、乙酸和苯并恶唑），支持了副产物的潜力。在生化甲烷电位测试中，预处理残渣的比甲烷电位为0.173 m³ CH4 kg⁻¹ VS，比非生物残渣增加85 %，高于未经处理的OPEFB。与Transference、Modified Gompertz和Logistic模型的动力学拟合结果吻合良好（R2高达0.92），其中Modified Gompertz模型最能捕获累积甲烷产量，滞后期最短。一个简单的能量检查表明了一个有利的余量：增加的甲烷（ΔSMP≈0.08 m³CH₄kg⁻¹VS）相当于0.8 kWh kg⁻¹ VS，而预处理的估计混合量为0.017-0.034 kWh kg⁻¹ VS。总的来说，C. testosterone预处理可以在中温条件下实现双重增值（生物甲烷和芳烃），为集成OPEFB生物精炼厂提供了一条实用的途径。

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