Biochemical Engineering Journal最新文献_第3页

Advances in bioactivities, cultivation strategies, genetic engineering, and multifaceted applications of Euglena gracilis 细叶菊的生物活性、栽培策略、基因工程及多方面应用研究进展

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-05-01 Epub Date: 2026-01-21 DOI: 10.1016/j.bej.2026.110087

Zhuoyao Li , Hongyu Zhang , Wenjun Song , Tingting Lou , Suying Wang , Sisi Niu , Jun Liu , Jin Hua

Euglena gracilis (E. gracilis) is a unique single-celled eukaryotic microalga that lacks a rigid cell wall, which endows it with remarkable morphological plasticity and environmental resilience. It has attracted extensive attention from both academia and industry owing to its rich repertoire of bioactive metabolites, exceptional adaptability to fluctuating environments, and broad biotechnological potential. E. gracilis synthesizes a wide range of bioactive components, including β-carotene, paramylon, vitamins, and fatty acids. Moreover, it uniquely combines the photosynthetic autotrophy typical of plant cells with the heterotrophic metabolism characteristic of animal cells, providing a model for cross-kingdom metabolic integration and enhancing its ecological competitiveness. However, despite its capability for autotrophic, heterotrophic, and mixotrophic cultivation, its large-scale commercial exploitation remains challenged by environmental sensitivity and limited market recognition. This review systematically outlines the morphology, bioactivities, and cultivation strategies of E. gracilis, critically assessing how growth conditions influence biomass and metabolite profiles. Furthermore, advances in genetic engineering aimed at enhancing strain robustness, productivity, and functional compound biosynthesis are reviewed, along with their emerging applications in food, health, bioenergy, and environmental fields. Collectively, this work highlights the value of E. gracilis as a multifunctional and sustainable biological platform, providing a scientific and practical foundation for its future industrial applications.

细叶藻（Euglena gracilis， E. gracilis）是一种独特的单细胞真核微藻，缺乏坚硬的细胞壁，这使其具有显著的形态可塑性和环境适应性。由于其丰富的生物活性代谢物、对波动环境的特殊适应性和广泛的生物技术潜力，它引起了学术界和工业界的广泛关注。薄叶菊合成了广泛的生物活性成分，包括β-胡萝卜素、paramylon、维生素和脂肪酸。此外，它独特地将植物细胞的光合自养特性与动物细胞的异养代谢特性结合起来，为跨界代谢整合提供了模型，增强了其生态竞争力。然而，尽管它具有自养、异养和混合养的能力，但其大规模商业开发仍然受到环境敏感性和市场认可度有限的挑战。这篇综述系统地概述了薄叶菊的形态、生物活性和培养策略，批判性地评估了生长条件如何影响生物量和代谢物特征。此外，综述了旨在提高菌株稳健性、生产力和功能性化合物生物合成的基因工程的进展，以及它们在食品、健康、生物能源和环境领域的新应用。综上所述，本研究突出了薄叶菊作为多功能可持续生物平台的价值，为其未来的工业应用提供了科学和实践基础。

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引用次数: 0

Enhanced physicochemical properties and tyrosinase inhibition of curcumin via glycosylation by Bacillus velezensis LQ5 in a non-aqueous system velezensis LQ5在非水体系中通过糖基化增强姜黄素的理化性质和酪氨酸酶抑制作用

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-05-01 Epub Date: 2026-02-05 DOI: 10.1016/j.bej.2026.110111

Jinhui Li , Zhihao He , Sen Zhang , Wenxin Ji, Yanqing Zhang, Junyun Guo, Jinao Duan, Lisi Zou, Liangliang Chen

The unique chemical structure of curcumin is a double-edged sword, responsible for its diverse bioactivities but also for its poor aqueous solubility and instability. To resolve this dilemma, a targeted structural modification strategy, microbial glycosylation, was employed. In this study, curcumin 4’-O-β-D-glucoside (CG) was biosynthesized in a non-aqueous system using Bacillus velezensis LQ5, introducing a glucose moiety without disrupting the essential pharmacophore of curcumin. Following the optimization of fermentation conditions, a molar yield of 55.48 % for CG was achieved within 9 h. This single glycosylation conferred concurrent and remarkable improvements in key properties: a dramatic 2153-fold enhancement in water solubility, superior photothermal stability (e.g., ∼65 % retention in solution at 100 °C for 6 h vs. < 40 % for curcumin), and a concurrently enhanced tyrosinase inhibitory activity (IC₅₀ = 119.15 μmol/L), representing an approximately ∼28 % increase in potency over curcumin, which confirms its place among active glycosides. This work validates microbial glycosylation as an effective strategy for optimizing property-limiting structures, offering a paradigm for the development of other insoluble natural products.

姜黄素独特的化学结构是一把双刃剑，它具有多种生物活性，但也具有较差的水溶性和不稳定性。为了解决这一难题，采用了一种靶向结构修饰策略——微生物糖基化。在本研究中，利用velezensis芽孢杆菌LQ5在非水体系中合成姜黄素4′-O-β- d -葡萄糖苷（CG），在不破坏姜黄素必需药理团的情况下引入葡萄糖片段。发酵条件优化后，CG的摩尔产率在9 h内达到55.48 %。这种单糖基化使其关键特性同时得到显著改善：水溶性显著提高2153倍，光热稳定性优越（例如，在100°C溶液中6 h的保留率为~ 65 %，姜黄素为40 %），酪氨酸酶抑制活性同时增强（IC50 = 119.15 μmol/L），其效力比姜黄素提高约28 %，这证实了其在活性苷中的地位。这项工作验证了微生物糖基化作为优化性能限制结构的有效策略，为开发其他不溶性天然产物提供了范例。

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引用次数: 0

Comparative analysis of free and CLEA-immobilised α-amylase and α-glucosidase from Anoxybacillus flavithermus 2641 T 黄热无氧杆菌2641 游离α-淀粉酶和α-葡萄糖苷酶与固定化α-淀粉酶的比较分析

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-05-01 Epub Date: 2026-01-30 DOI: 10.1016/j.bej.2026.110106

Emel Alemdaroğlu , Fulya Ay , Dilşat Nigar Çolak , Halil İbrahim Güler , Ali Osman Belduz

α-Amylase (EC 3.2.1.1) and α-glucosidase (EC 3.2.1.20) are key enzymes in starch hydrolysis, widely applied in biotechnological and food industries. In this study, recombinant α-amylase (AflAmy) and α-glucosidase (AflGlu) from Anoxybacillus flavithermus 2641 ^T were purified using a cobalt affinity column, yielding proteins of approximately 50 kDa as confirmed by SDS-PAGE. Both enzymes were immobilised through the cross-linked enzyme aggregate (CLEA) method. Optimal CLEA preparation involved 96 % ammonium sulfate saturation at 4 °C for 30 min, followed by cross-linking with 5 mM glutaraldehyde for 2–3 h at room temperature. Free AflAmy exhibited optimal activity at pH 8.0 and 70 °C, while immobilization shifted its optimum to pH 9.0. Free AflGlu was most active at pH 8.0 and 60 °C, changing to 55 °C upon immobilization. CLEA forms displayed lower Km value for AflAmy-IM, indicating increased substrate affinity. Reusability tests showed immobilised AflAmy retained activity over nine cycles, AflGlu over three, and their combined form (Combi-CLEA) up to nine cycles. Thermal stability of immobilised AflAmy improved, maintaining activity for 150 min at 70 °C. Both free and immobilized forms of the enzymes achieved ∼50 % starch hydrolysis within 60 min, demonstrating comparable catalytic efficiency and enhanced operational stability after immobilization.

α-淀粉酶（EC 3.2.1.1）和α-葡萄糖苷酶（EC 3.2.1.20）是淀粉水解的关键酶，广泛应用于生物技术和食品工业。本研究利用钴亲和柱纯化了黄热无氧杆菌2641 T中的重组α-淀粉酶（AflAmy）和α-葡萄糖苷酶（AflGlu）， SDS-PAGE证实，所得蛋白约为50 kDa。两种酶通过交联酶聚集体（CLEA）法固定。最佳的CLEA制备方法是：96 %硫酸铵在4°C下饱和30 min，然后与5 mM戊二醛在室温下交联2-3 h。游离AflAmy在pH 8.0和70°C时表现出最佳活性，而固定化则在pH 9.0时表现出最佳活性。游离AflGlu在pH 8.0和60°C时最具活性，固定后为55°C。CLEA形式对AflAmy-IM显示较低的Km值，表明底物亲和力增加。可重用性测试表明，固定化AflAmy可保持9个循环的活性，AflGlu可保持3个循环，而它们的组合形式（Combi-CLEA）可保持9个循环。固定化AflAmy的热稳定性得到改善，在70°C下保持150 min的活性。游离形式和固定化形式的酶都在60 min内实现了~ 50% %的淀粉水解，显示出相当的催化效率和固定化后增强的操作稳定性。

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引用次数: 0

Comparative study of biofilter and continuous stirred tank reactor for anammox fast start-up and stable operation 厌氧氨氧化生物滤池与连续搅拌槽反应器快速启动稳定运行的对比研究

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-05-01 Epub Date: 2026-02-09 DOI: 10.1016/j.bej.2026.110117

Longyan Chang , Dingying Ning , Mingyue Li , Wenqing Tian , Chu Wang , Fuxiong Nan , Di Wu , Jidong Liang , Wei Yan

The performance of anaerobic ammonium oxidation (anammox) in mainstream wastewater treatment is often limited by slow start-up and biomass retention challenges. In this study, biofilter (BF) and continuous stirred-tank reactor (CSTR) configurations were evaluated for anammox cultivation, focusing on start-up performance, nitrogen removal, and microbial dynamics. The results showed that the BF achieved higher total nitrogen removal rate (TNRE) of 79.35 % ± 0.63 % and nitrogen removal rate (NRR) of 1.35 ± 0.04 kg N/(m³·d) than 74.99 ± 3.53 % of TNRE and 0.59 ± 0.04 kg N/(m³·d) of NRR in CSTR. The nitrogen removal of BF driven by the combined contribution of anammox, DN and PN/A. CSTR achieved a shorter start-up time within 34 days and a higher anammox nitrogen removal contribution of 92.22 ± 4.50 % than 59 days for start-up and 84.65 ± 4.80 % anammox nitrogen removal contribution of BF, reflecting the predominance of the anammox pathway. BF showed vertical stratification of nitrogen removal, whereas CSTR formed stable granular sludge with high activity. Moreover, microbial analysis revealed distinct enrichment patterns, with Candidatus Brocadia dominating in BF and Candidatus Kuenenia in CSTR, reflecting niche differentiation driven by reactor configuration. These findings highlight the influence of reactor design on anammox activity, microbial community formation, and nitrogen removal efficiency, providing insights for optimizing mainstream anammox applications in wastewater treatment.

厌氧氨氧化（anammox）在主流废水处理中的性能往往受到启动缓慢和生物质保留挑战的限制。在这项研究中，生物过滤器（BF）和连续搅拌槽反应器（CSTR）配置对厌氧氨氧化菌的培养进行了评估，重点关注启动性能、氮去除和微生物动力学。结果表明,高炉实现更高的总氮去除率(TNRE)为79.35±0.63  % %和氮去除率 (NRR) 1.35±0.04  公斤 N / (m3·d)超过74.99 ±3.53  % TNRE和0.59±0.04  公斤 N / (NRR m3·d)在装运箱。厌氧氨氧化、DN和PN/A共同作用下的高炉脱氮效果。CSTR在34 d内启动时间较短，厌氧氨氧化氮去除贡献为92.22±4.50 %，高于启动59 d和BF厌氧氨氧化氮去除贡献84.65 ± 4.80 %，体现了厌氧氨氧化途径的优势。高炉脱氮表现为垂直分层，而CSTR脱氮表现为稳定的高活性颗粒污泥。此外，微生物分析显示出不同的富集模式，在BF中以Candidatus Brocadia为主，在CSTR中以Candidatus Kuenenia为主，反映了反应器配置驱动的生态位分化。这些发现突出了反应器设计对厌氧氨氧化活性、微生物群落形成和脱氮效率的影响，为优化厌氧氨氧化在废水处理中的主流应用提供了见解。

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引用次数: 0

Platelet membrane-modified liquid metal nanoparticles enhanced cellular uptake and tumor photothermal therapy 血小板膜修饰的液态金属纳米颗粒增强细胞摄取和肿瘤光热治疗

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-05-01 Epub Date: 2026-02-03 DOI: 10.1016/j.bej.2026.110108

Siyuan Pang , Zhihang Wang , Yang Zou , Li Zhang , Yonggang Lv

Liquid metal (LM) nanoparticles have been widely used in photothermal therapy (PTT), but they are susceptible to oxidative inactivation and have poor targeting ability. Platelets (PLT) have many abundant membrane proteins on their surface that can be used to modify LM nanoparticles. Here, a gallium (Ga)-based LM-based nanoparticle delivery system was developed. The conducting polymer polypyrrole (PPy) was first grown in situ on the surface of LM nanoparticles by polymerization (named as LM@PPy). The oxidation resistance and photothermal stability of LM were improved. Subsequently, PLT membrane (PM) was extracted and coated on the surface of LM@PPy to prepare LM@PPy/PM. The antitumor effect of LM@PPy/PM was investigated through in vitro and in vivo experiments. It was demonstrated that the LM@PPy had better photothermal stability and their photothermal conversion efficiency reached 55 ± 2 %, which was higher than that of unmodified LM nanoparticles (31 ± 2 %). Most of the membrane proteins from PLT were retained on the prepared LM@PPy/PM. The PM coating effectively enhanced the tumor-targeting ability of the nanoparticles, leading to better tumor accumulation and antitumor effects in in vitro and in vivo. The findings showed that this nanoparticle delivery system provided a new technological solution to improve the antitumor ability of LM nanoparticles.

液态金属（LM）纳米颗粒在光热治疗（PTT）中得到了广泛的应用，但其易氧化失活且靶向能力差。血小板（PLT）表面有许多丰富的膜蛋白，可用于修饰LM纳米颗粒。本文开发了一种基于镓（Ga）的纳米颗粒递送系统。首先通过聚合在LM纳米颗粒表面原位生长导电聚合物聚吡咯（PPy）（命名为LM@PPy）。提高了LM的抗氧化性和光热稳定性。随后，提取PLT膜（PM），涂覆在LM@PPy表面，制备LM@PPy/PM。通过体外和体内实验考察LM@PPy/PM的抗肿瘤作用。结果表明，LM@PPy具有较好的光热稳定性，其光热转换效率达到55 ± 2 %，高于未修饰的LM纳米粒子（31 ± 2 %）。大部分来自PLT的膜蛋白保留在制备的LM@PPy/PM上。PM包被有效地增强了纳米颗粒的肿瘤靶向能力，从而在体外和体内具有更好的肿瘤蓄积和抗肿瘤作用。研究结果表明，该纳米颗粒递送系统为提高LM纳米颗粒的抗肿瘤能力提供了新的技术解决方案。

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引用次数: 0

Application of fine-grained sulfur particles on sulfur autotrophic denitrification process: Evaluation of performance benefits, cold-tolerant mechanism and operational challenges 细粒硫颗粒在硫自养反硝化过程中的应用：性能效益评价、耐寒机制和操作挑战

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-05-01 Epub Date: 2026-02-10 DOI: 10.1016/j.bej.2026.110119

Mingdong Chang , Kuo Zhang , Tong Zhu , Chunli Zheng , Tangfu Xiao , Jianqiao Wang , Feng Ma , Zhitie Chang , Youzhao Wang

The poor sulfur bioavailability poses a key constraint on the practical application of sulfur autotrophic denitrification (SAD). This study employed fine-grained sulfur particles to address this limitation, evaluating their impact on denitrification performance, clogging risk, sulfur loss, and low-temperature adaptability. Results showed that fine particles significantly enhanced biomass accumulation and nitrate reductase content, improving system tolerance to loading shocks. Response surface methodology identified optimal conditions for the fine-particle reactor as a 1.5 h HRT, pH 7.0, and 0.5 mg/L dissolved oxygen concentration, achieving 82.8 % denitrification efficiency and a nitrogen removal rate of 0.473 kg/(m³·d). However, finer particles also increased the biomass growth rate by approximately 10 % and raised abiotic loss of sulfur particles by 84.8 %, indicating heightened risks of reactor clogging and sulfur washout. Microbial community analysis under low-temperature operation revealed that while SAD bacteria remained dominant, the increased content of extracellular polymeric substances (EPS) substantially elevated the abundance of endogenous heterotrophic denitrifiers. The protective role of EPS, combined with heterotrophic metabolic compensation, jointly supported stable nitrogen removal at low temperatures. In summary, despite associated challenges like clogging and sulfur loss, the fine-particle reactor demonstrated superior adaptability and operational stability under dynamic environmental and variable water quality conditions compared to conventional SAD filters. This offers a promising strategy for advancing the engineering application of SAD technology.

硫生物利用度差是制约硫自养反硝化（SAD）实际应用的关键因素。本研究采用细粒硫颗粒来解决这一限制，评估其对脱硝性能、堵塞风险、硫损失和低温适应性的影响。结果表明，细颗粒显著提高了生物量积累和硝酸还原酶含量，提高了系统对负荷冲击的耐受性。响应面法确定细颗粒反应器的最佳条件为：HRT为1.5 h， pH为7.0，溶解氧浓度为0.5 mg/L，反硝化效率为82.8 %，氮去除率为0.473 kg/（m3·d）。然而，更细的颗粒也使生物量增长率提高了约10% %，并使硫颗粒的非生物损失率提高了84.8% %，这表明反应器堵塞和硫冲刷的风险增加。低温条件下微生物群落分析表明，虽然SAD菌仍占主导地位，但胞外聚合物质（EPS）含量的增加显著提高了内源性异养反硝化菌的丰度。EPS的保护作用与异养代谢补偿相结合，共同支持了低温下稳定的脱氮。总之，尽管存在堵塞和硫损失等相关挑战，但与传统的SAD过滤器相比，细颗粒反应器在动态环境和可变水质条件下表现出更强的适应性和运行稳定性。这为推进SAD技术的工程应用提供了一个有希望的策略。

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引用次数: 0

Quantifying the rate-limiting step and microbial stratification in linear alkylbenzene sulfonate biodegradation within a pilot-scale anaerobic-aerobic biofilm system 在中试厌氧-好氧生物膜系统中定量线性烷基苯磺酸生物降解的限速步骤和微生物分层

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-05-01 Epub Date: 2026-02-09 DOI: 10.1016/j.bej.2026.110115

Huiqing Lou , Xianzhong Cao

The widespread discharge of linear alkylbenzene sulfonate (LAS) into wastewater poses significant challenges for conventional treatment systems. This study provides a quantitative, multi-level mechanistic investigation into LAS biodegradation within a novel pilot-scale anaerobic filter–biological contact oxidation (AF–BCO) stratified biofilm system. Under optimized conditions (total HRT 24 h, DO 4.0–5.0 mg/L, 30–35 ℃), the system achieved stable removal efficiencies of 98.2 % for LAS and 96.1 % for COD. Crucially, metabolic flux analysis quantitatively identified alkyl chain β-oxidation as the rate-limiting step, with a characteristic sulfonate hydrolysis-to-β-oxidation flux ratio of 3.2:1, providing a kinetic rationale for process design beyond empirical optimization. High-throughput sequencing and metagenomics revealed a spatially orchestrated metabolic division of labor: Desulfobacter (18.7 %) dominated the anaerobic biofilm, correlating with high suyAB gene abundance and sulfonatase activity for initial sulfonate cleavage, while Pseudomonas (22.1 %) prevailed in the aerobic biofilm, aligning with elevated CYP450 gene expression and monooxygenase activity for ring mineralization. Kinetic modeling validated substrate and oxygen utilization patterns, and the system demonstrated remarkable resilience, fully recovering treatment efficiency within 48 h after a severe shock load (250 mg/L LAS). By integrating metabolic fluxes, microbial ecology, molecular biology, and reactor kinetics, this work establishes a predictive, mechanistic framework for the design of stratified biofilm systems treating surfactant-laden wastewater.

线性烷基苯磺酸盐（LAS）广泛排放到废水中，对传统处理系统提出了重大挑战。本研究对新型中试厌氧过滤器-生物接触氧化（AF-BCO）分层生物膜系统中LAS的生物降解进行了定量、多层次的机理研究。在总HRT 24 h， DO 4.0 ~ 5.0 mg/L， 30 ~ 35℃条件下，系统对LAS的去除率为98.2% %，对COD的去除率为96.1% %。至关重要的是，代谢通量分析定量地确定烷基链β-氧化为限速步骤，其特征是磺酸水解与β-氧化通量比为3.2:1，为超越经验优化的工艺设计提供了动力学基础。高通量测序和宏基因组学揭示了一个空间协调的代谢分工：在厌氧生物膜中，脱硫杆菌（18.7 %）占主导地位，与高suyAB基因丰度和磺化酶活性相关，与初始磺化酶活性相关，而在好氧生物膜中，假单胞菌（22.1 %）占主导地位，与CYP450基因表达和单加氧酶活性升高一致，用于环矿化。动力学模型验证了底物和氧气利用模式，系统显示出显著的弹性，在剧烈冲击负荷（250 mg/L LAS）后48 h内完全恢复处理效率。通过整合代谢通量、微生物生态学、分子生物学和反应器动力学，本研究为设计处理表面活性剂废水的分层生物膜系统建立了一个预测性的机制框架。

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引用次数: 0

One-pot preparation of magnetic cross-linked enzyme aggregates from a crude enzyme cocktail for cascaded catalytic production of biliverdin 用粗酶混合物一锅制备磁性交联酶聚集体用于级联催化生产胆绿素

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-05-01 Epub Date: 2026-02-12 DOI: 10.1016/j.bej.2026.110116

Xiyuan Tang , Meiying Zhu , Zhipeng Liu , Longyu Zhang , Chengli Yang , Jian Zhang , Zhaohui Liu , Dali Li

Immobilized enzyme technology has been widely used because of its advantages in enhancing enzyme stability and reusability. However, conventional methods rely on highly purified enzymes, which involve tedious extraction steps and high costs, thereby limiting their practical applications. In recent years, direct immobilization strategy based on crude enzyme has garnered attention due to its economy and high efficiency. This study developed a one-pot co-immobilization strategy where crude enzymes of recombinant cytochrome P450 reductase (rCPR), recombinant glucose dehydrogenase (rGDH), and recombinant heme oxygenase (rHO) were mixed with genipin and aminated magnetic nanoparticles to prepare magnetic cross-linked enzyme aggregates (MCLEAs) for the cascade catalysis of heme to biliverdin. After immobilization, the immobilization efficiency of rCPR, rGDH and rHO reached 64.3 %, 70.6 % and 95.2 %, respectively. The activity recovery of rCPR, rGDH, and rHO reached 105.0 %, 65.0 % and 120.0 %, respectively. The thermal and pH stability of the enzymes in MCLEAs were greatly improved, while excellent reusability and storage stability were achieved. After 7 catalytic cycles and 30 days of storage, the MCLEAs retained 50.0 % and 35.3 % of the residual activity, respectively. The cascade system significantly enhanced the catalytic performance of rHO, achieving a biliverdin production of 0.70 μM after 10 min of reaction. This study offers a novel strategy for the simple immobilization and efficient application of complex enzyme systems.

固定化酶技术由于具有提高酶的稳定性和可重复利用性等优点而得到了广泛的应用。然而，传统的方法依赖于高纯度的酶，这涉及繁琐的提取步骤和高昂的成本，从而限制了它们的实际应用。近年来，基于粗酶的直接固定化策略因其经济性和高效性而备受关注。本研究开发了一锅共固定化策略，将重组细胞色素P450还原酶（rCPR）、重组葡萄糖脱氢酶（rGDH）和重组血红素加氧酶（rHO）粗酶与吉尼平和胺化磁性纳米颗粒混合，制备磁性交联酶聚集体（MCLEAs），用于血红素级联催化合成胆绿素。固定化后，rCPR、rGDH和rHO的固定化效率分别达到64.3 %、70.6 %和95.2% %。rCPR、rGDH和rHO的活性回收率分别为105.0 %、65.0 %和120.0 %。在MCLEAs中，酶的热稳定性和pH稳定性大大提高，并且具有良好的可重复使用性和储存稳定性。经过7个催化循环和30天的储存，mclea的残余活性分别保持了50.0 %和35.3 %。级联体系显著提高了rHO的催化性能，反应10 min后胆绿素的产率达到0.70 μM。本研究为复杂酶体系的简单固定和高效应用提供了一种新的策略。

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引用次数: 0

A first newly isolated strain capable of effectively degrading 3-methylpentane: Identification, degradation characteristics and pathway analysis 一个新分离的能有效降解3-甲基戊烷的菌株：鉴定、降解特性和途径分析

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-05-01 Epub Date: 2026-01-19 DOI: 10.1016/j.bej.2026.110084

Qian Li , Yujing Zhao , Liqin Sun , Zhonghui Sun , Raquel Lebrero , Raúl Muñoz , Jianmeng Chen , Rongsheng Lin , Dongzhi Chen

Biotechnology is applicable for the treatment of volatile organic compounds emitted from petrochemical industries. However, the biodegradation of methyl alkanes is limited by low microbial activity and poor mass transfer. Herein, a novel strain capable of effectively utilizing 3-methylpentane (3MP) as the sole carbon source was firstly isolated and identified as Mycobacterium sp. SLQ-1. The strain could function well within wide ranges of pH (5.0–8.0) and temperature (30–45℃), with the maximum specific growth and biodegradation rates being 0.084 and 0.587 h⁻¹, respectively. The optimum function conditions (pH 6.9, temperature 36.2℃) and 3MP removal efficiencies were predicted via response surface analysis. The repeated recycling of 2-methyl silicone oil enhanced 3MP biodegradation due to microbial adsorption. Two possible 3MP biodegradation pathways were proposed based on the analysis of metabolic intermediates, genes and enzymes. Furthermore, the strain had a wide utilization spectrum toward various alkanes, olefins and aromatics, presenting great engineering application potential.

生物技术适用于石油化工排放的挥发性有机物的处理。然而，甲基烷烃的生物降解受到微生物活性低和传质差的限制。本文首次分离到一株能有效利用3-甲基戊烷（3MP）作为唯一碳源的新菌株，并鉴定为分枝杆菌sp. SLQ-1。菌株在较宽的pH（5.0 ~ 8.0）和温度（30 ~ 45℃）范围内均能很好地生长，其最大比生长率和生物降解率分别为0.084和0.587 h−1。通过响应面分析，预测了最佳作用条件（pH 6.9，温度36.2℃）和3MP去除效率。2-甲基硅油的重复循环利用通过微生物吸附增强了3MP的生物降解。通过对代谢中间体、基因和酶的分析，提出了两种可能的3MP生物降解途径。该菌株对各种烷烃、烯烃和芳烃具有广泛的利用谱，具有很大的工程应用潜力。

{"title":"A first newly isolated strain capable of effectively degrading 3-methylpentane: Identification, degradation characteristics and pathway analysis","authors":"Qian Li , Yujing Zhao , Liqin Sun , Zhonghui Sun , Raquel Lebrero , Raúl Muñoz , Jianmeng Chen , Rongsheng Lin , Dongzhi Chen","doi":"10.1016/j.bej.2026.110084","DOIUrl":"10.1016/j.bej.2026.110084","url":null,"abstract":"<div><div>Biotechnology is applicable for the treatment of volatile organic compounds emitted from petrochemical industries. However, the biodegradation of methyl alkanes is limited by low microbial activity and poor mass transfer. Herein, a novel strain capable of effectively utilizing 3-methylpentane (3MP) as the sole carbon source was firstly isolated and identified as <em>Mycobacterium</em> sp. SLQ-1. The strain could function well within wide ranges of pH (5.0–8.0) and temperature (30–45℃), with the maximum specific growth and biodegradation rates being 0.084 and 0.587 h<sup>−1</sup>, respectively. The optimum function conditions (pH 6.9, temperature 36.2℃) and 3MP removal efficiencies were predicted via response surface analysis. The repeated recycling of 2-methyl silicone oil enhanced 3MP biodegradation due to microbial adsorption. Two possible 3MP biodegradation pathways were proposed based on the analysis of metabolic intermediates, genes and enzymes. Furthermore, the strain had a wide utilization spectrum toward various alkanes, olefins and aromatics, presenting great engineering application potential.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"229 ","pages":"Article 110084"},"PeriodicalIF":3.7,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146006642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Limit of hydrogen addition for enhanced methane concentration and production during syngas biomethanation in thermophilic trickle-bed reactor 嗜热滴床反应器合成气生物甲烷化过程中加氢提高甲烷浓度和产量的限制

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2026-05-01 Epub Date: 2026-02-05 DOI: 10.1016/j.bej.2026.110112

Florian Gabler , George Cheng , Leticia Pizzul , Anna Schnürer , Åke Nordberg

Syngas biomethanation is an emerging technology that converts synthesis gas, primarily composed of hydrogen (H₂), carbon monoxide (CO), and carbon dioxide (CO₂), into methane (CH₄) through microbial activity. In this study, the effect of changing syngas composition with increased H₂ shares on CH₄ concentration and production was assessed for 125 days, using a thermophilic trickle-bed reactor (5 L). With the experimental upper limit of 71 % H₂ (14 % CO, 10 % CO₂, 5 % N₂) in the syngas, the maximum CH₄ concentration was 65 %, maintaining high methane evolution rates (4 L/(L_pbv·d)) and high H₂ and CO conversion rates (>95 %). Targeted sulfur supplementation (Na₂S) did not improve H₂ and CO conversion or CH₄ productivity, indicating that sulfur was no limiting factor under digestate-based operation. Reactor performance was instead constrained by system-level factors, including low gas retention time, gas–liquid mass transfer limitations, and inhibition of CO-converting pathways at elevated H₂ partial pressure. 16S rRNA gene sequencing revealed a highly stable microbial community dominated by the hydrogenotrophic methanogen Methanothermobacter. CO conversion occurred via direct methanogenesis and acetate formation, followed by syntrophic acetate oxidation. Overall, increasing H₂ availability enhanced CH₄ production only up to a system-specific threshold, beyond which microbial and transport limitations dominated.

合成气生物甲烷化是一项新兴技术，通过微生物活动将主要由氢气（H2）、一氧化碳（CO）和二氧化碳（CO2）组成的合成气转化为甲烷（CH4）。在这项研究中，使用一个亲热滴床反应器（5 L），评估了125天内合成气组成随H2份额的增加对CH4浓度和产量的影响。在实验上限为71 % H2（14 % CO, 10 % CO2, 5 % N2）的合成气中，CH4的最大浓度为65 %，保持了较高的甲烷演化率（4 L/(Lpbv·d)）和较高的H2和CO转化率（95 %）。定向补硫（Na2S）并没有提高H2和CO转化率，也没有提高CH4生产率，说明在以消化物为基础的操作中，硫不是限制因素。相反，反应器性能受到系统级因素的限制，包括低气体滞留时间、气液传质限制以及在高H2分压下co转化途径的抑制。16S rRNA基因测序显示，该微生物群落高度稳定，以氢营养产甲烷菌（Methanothermobacter）为主。CO的转化主要通过直接产甲烷和醋酸盐的生成，然后是醋酸盐的合成氧化。总的来说，增加H2可用性只会在系统特定阈值范围内提高CH4产量，超过该阈值，微生物和运输限制占主导地位。

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引用次数: 0