Biochemical Engineering Journal最新文献_第3页

Comparative evaluation of a hybrid membrane treatment train versus full-scale aerobic/anoxic activated sludge system: C-N removal performances, syntrophic microbial dynamics, and energy savings 混合膜处理系统与全规模好氧/缺氧活性污泥系统的比较评价：C-N去除性能、共生微生物动力学和节能

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-12-16 DOI: 10.1016/j.bej.2025.110052

Santhana Krishnan , Araya Thongsai , Pongsak (Lek) Noophan , Muhammad Ahmar Siddiqui , Yee-Shian Wong , Jaeho Bae , Sumate Chaiprapat

This study presents a novel hybrid membrane treatment train that integrates an anaerobic membrane bioreactor (AnMBR) with a fluidized bed membrane bioreactor enriched with partial nitritation and anammox (FMBR^PN/A) for the treatment of industrial wastewater. The system was evaluated in terms of pollutant removal performance, microbial community structure, and energy efficiency, and benchmarked against a conventional full-scale aerobic/anoxic activated sludge system with an integrated constructed wetland (AS^N/DN+Wetland). The AnMBR+FMBR^PN/A achieved high removal efficiencies for COD (91.1 %) and total nitrogen (70.1 %), matching the performance of the conventional system while operating under low-carbon effluent conditions. Strategic aeration of FMBR^PN/A enabled spatial separation of partial nitritation and anammox zones, promoting syntrophic coexistence of ammonium-oxidizing (AOB), nitrite-oxidizing (NOB), denitrifying (DN), and anammox (AMX) microbes in direct contact with the membrane surface, contrasted with microbial dynamics in traditional systems. The AnMBR+FMBR^PN/A process had better energy performance, with 26.6 % reduction in electricity consumption (0.55 vs. 0.74 kWh/m³) and added benefits in biomethane recovery and reduced greenhouse gas emissions. These findings point to the potential of hybrid membrane bioreactors to transform industrial nitrogen management towards more efficient and sustainable solutions.

本研究提出了一种新型的混合膜处理系统，该系统将厌氧膜生物反应器（AnMBR）与富部分硝化和厌氧氨氧化的流化床膜生物反应器（FMBRPN/ a）集成在一起，用于工业废水的处理。该系统在污染物去除性能、微生物群落结构和能源效率方面进行了评估，并与传统的全规模好氧/缺氧活性污泥系统（ASN/DN+湿地）进行了基准测试。在低碳出水条件下，AnMBR+FMBRPN/A对COD（91.1 %）和总氮（70.1 %）的去除率达到了常规系统的水平。与传统系统的微生物动力学相比，FMBRPN/A的策略曝气使部分硝化和厌氧氨氧化区在空间上分离，促进氨氧化（AOB）、亚硝酸盐氧化（NOB）、反硝化（DN）和厌氧氨氧化（AMX）微生物与膜表面直接接触的共生共存。AnMBR+FMBRPN/A工艺具有更好的能源性能，可减少26.6% %的用电量（0.55 vs. 0.74 kWh/m3），并增加了生物甲烷回收和减少温室气体排放的效益。这些发现表明，混合膜生物反应器有潜力将工业氮管理转变为更有效和可持续的解决方案。

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引用次数: 0

Effects of high-temperature fermentation liquid and mechanically dewatering liquid on algal-bacterial granular sludge: Granular characteristics, pollutant removal and algal-bacterial structure 高温发酵液和机械脱水液对藻菌颗粒污泥的影响：颗粒特性、污染物去除和藻菌结构

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-12-15 DOI: 10.1016/j.bej.2025.110028

Ao Wang , Peng-fei Yu , Ding Wang , Hai-wen Sun , Bo-mei Hu , Xing-guan Ma , Shi-gang Tu

Lowering water content and pollutant concentration in sludge is key for sludge reduction and resource reuse. However, the waste liquid generated during this process is highly concentrated, imposing additional environmental burdens. To address this issue, an Algae-bacteria granular sludge (ABGS) system was constructed. This study compared and analyzed pollutant removal performance and microbial interactions within the ABGS when treating both sludge high-temperature fermentation Liquid (HFL) and mechanical dewatering filtrate (MDF). The results showed: The ABGS achieved high removal efficiencies for Total Organic Carbon (950 and 800 mg/L), Total Nitrogen (230 and 130 mg/L), and Phosphorus (65 and 3 mg/L) in both the HFL and the MDF, with rates reaching 93.35 %, 98.07 %, 96.35 % for HFL and 98.18 %, 86.12 %, 89.81 % for MDF, respectively. Both actual wastewater enhanced extracellular polymeric substances (295.56, 315.81 mg/g VSS), integrity coefficient, granular size, and MLVSS/MLSS ratio (than 0.75), improved granular strength of the ABGS.A high-concentration environment of carbon, nitrogen, and phosphorus may activate algal-bacterial cometabolic networks. Microalgae release dissolved oxygen via photosynthesis, enhancing the activity of nitrifying bacteria (Pararhodobacter). Functional complementarity occurs between polyphosphate accumulating organisms such as Paracoccaceae and denitrifiers like Thauera. Through quorum sensing, key metabolic genes (pst, pts, nir, nor) are regulated, establishing a coupled system integrating photosynthetic oxygen supply with simultaneous nitrogen and phosphorus removal.

降低污泥含水量和污染物浓度是污泥减量化和资源化利用的关键。然而，在此过程中产生的废液高度集中，增加了环境负担。为解决这一问题，构建了藻类-细菌颗粒污泥（ABGS）系统。本研究对比分析了处理污泥高温发酵液（HFL）和机械脱水滤液（MDF）时ABGS内的污染物去除性能和微生物相互作用。结果显示:abg实现高去除效率总有机碳(950年和800年 mg / L)、总氮(230年和130年 mg / L),和磷(65和3 mg / L) hf和MDF, %率达到93.35,98.07 % 96.35 hf和98.18 % %, % 86.12,89.81为MDF %,分别。实际废水均增强了胞外聚合物（295.56、315.81 mg/g VSS）、完整性系数、粒径和MLVSS/MLSS比（大于0.75），提高了ABGS的颗粒强度。高浓度的碳、氮、磷环境可能激活藻类-细菌共代谢网络。微藻通过光合作用释放溶解氧，提高硝化细菌（副嗜酸杆菌）的活性。功能互补发生在聚积多磷酸盐的生物如副球菌科和反硝化菌如Thauera之间。通过群体感应调节关键代谢基因（pst、pts、nir、nor），建立光合供氧与同时脱氮除磷的耦合系统。

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引用次数: 0

Insight into the enhanced cAMP synthesis using Arthrobacter sp. CCTCC 2013431 under polyphosphate addition condition based on physiological and transcriptome analyses 基于生理和转录组分析，深入了解在多磷酸盐添加条件下使用Arthrobacter sp. CCTCC 2013431增强cAMP合成

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-12-15 DOI: 10.1016/j.bej.2025.110048

Zhigang Li , Nanxun Lu , Ruifang Li , Wei Liu , Chang Li , Zhonghua Zhang , Tianyou Yang

The insufficient supply of ATP limited cAMP synthesis during fermentations using Arthrobacter sp. CCTCC 2013431. cAMP fermentations with/without hexametaphosphate addition were conducted in a 7 L bioreactor and the underlying mechanism for enhanced cAMP biosynthesis under hexametaphosphate addition condition was investigated. Due to the addition of 2 g/L-broth sodium hexametaphosphate at 24 h, cAMP content reached 3.64 g/L, with an increment of 33.82 % when compared with control. The transcriptome analysis results revealed that 227 genes were up-regulated and 265 genes were down-regulated significantly in response to hexametaphosphate addition. For glycometabolism, the transcription levels of key enzymes genes involved with cAMP synthesis were enhanced significantly, and for energy metabolism, the transcription levels of genes associated with electron transport chain and polyphosphate kinase gene were increased significantly by which sufficient carbon skeleton and ATP were provided for cAMP biosynthesis. In addition, transcription levels of most reductase genes were up regulated significantly suggesting that intracellular antioxidant capacity was improved effectively by hexametaphosphate. Finally, activities of key enzymes together with intracellular contents of ROS, MDA and key metabolites were detected and the results were in accordance to those of transcriptome analysis. It could be concluded that hexametaphosphate relieved severe oxidative stress, strengthened energy metabolism and redistributed the carbon flux among different pathways resulting in enhanced cAMP synthesis and decreased byproduct generation.

在利用关节杆菌sp. CCTCC 2013431发酵过程中，ATP供应不足限制了cAMP的合成。在一个7 L的生物反应器中进行了有/没有六偏磷酸的cAMP发酵，并研究了六偏磷酸条件下cAMP生物合成增强的潜在机制。24 h加入2 g/L-六偏磷酸钠，cAMP含量达到3.64 g/L，比对照增加33.82 %。转录组分析结果显示，六偏磷酸酯添加显著上调了227个基因，下调了265个基因。糖代谢方面，参与cAMP合成的关键酶基因转录水平显著提高；能量代谢方面，电子传递链和多磷酸激酶相关基因转录水平显著提高，为cAMP生物合成提供了充足的碳骨架和ATP。此外，大多数还原酶基因的转录水平显著上调，表明六偏磷酸有效提高了细胞内抗氧化能力。最后检测关键酶活性、细胞内ROS、MDA及关键代谢物含量，结果与转录组分析结果一致。综上所述，六偏磷酸盐缓解了严重的氧化应激，加强了能量代谢，并在不同途径之间重新分配了碳通量，从而提高了cAMP的合成，减少了副产物的产生。

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引用次数: 0

Hydrocyclone-enhanced nutrient removal and sludge densification in activated sludge system: Insights into microbial response 活性污泥系统中水力旋流器增强的营养物去除和污泥致密化：对微生物反应的见解

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-12-15 DOI: 10.1016/j.bej.2025.110051

Wentao Xu , Shasha Liu , Mei Sun , Shijie Sun , Jiao Wang , Zhiming Bian , Qingyun Zhang , Changsheng Peng

Sludge densification is a promising technology to enhance nutrient removal in existing municipal wastewater treatment plants (WWTPs). In this study, the graded-device hydrocyclone was installed into activated sludge system to assist sludge densification and disclose its microbial response mechanism. Higher nutrient removal (94.2 % for COD, 97.2 % for NH₄⁺-N, 82.3 % for TN, 92.4 % for TP, respectively) were achieved in R2 reactor incorporating hydrocyclone, and these effluent indicators met the wastewater first class A discharge standards of China (GB18918–2002). The hydraulic shear forces of hydrocyclone promoted the sludge transformation from loose flocs to dense particles (average size of 292 μm), sludge settleability (relative hydrophobicity of 79.1 %, SVI₃₀ of 60 mL/g), and the secretion of extracellular polymeric substances (EPS of 98.89 mg/g-SS, protein/polysaccharide ratio of 1.78). Ammonia-oxidizing bacteria, nitrite-oxidizing bacteria, denitrifying bacteria and polyphosphate-accumulating organisms were effectively enriched. These functional bacteria actively participated in membrane transport, signal transduction, carbon metabolism, nitrogen metabolism, phosphonate and phosphinate metabolism, providing the core driving force for long-term stable nutrient removal. These findings provided feasibility and mechanism support for hydrocyclone in enhancing nutrient removal, with a view to achieving cost reduction and efficiency benefits via sludge densification in industrial applications at full-scale WWTPs within the environmental field.

污泥致密化是一种很有前途的技术，可以提高现有城市污水处理厂的营养物去除率。本研究将分级装置水力旋流器安装在活性污泥系统中，辅助污泥致密化，揭示其微生物响应机理。采用水力旋流器的R2反应器，COD去除率为94.2 %，NH4+-N去除率为97.2% %，TN去除率为82.3 %，TP去除率为92.4 %，出水指标均达到中国废水一级A排放标准（GB18918-2002）。水力旋流器的水力剪切力促进了污泥由松散絮体向致密颗粒（平均粒径为292 μm）的转化，污泥的沉降性（相对疏水性为79.1% %，SVI30为60 mL/g），以及细胞外高分子物质的分泌（EPS为98.89 mg/g- ss，蛋白/多糖比为1.78）。氨氧化菌、亚硝酸盐氧化菌、反硝化菌和聚磷菌得到有效富集。这些功能性细菌积极参与膜转运、信号转导、碳代谢、氮代谢、膦酸盐和膦酸盐代谢，为长期稳定的营养物去除提供核心动力。这些发现为水力旋流器加强营养物去除提供了可行性和机制支持，以期在环境领域的大规模污水处理厂的工业应用中通过污泥致密化实现成本降低和效率提高。

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引用次数: 0

Leveraging histidine-nickel coordination for stable enzyme immobilization on metal-organic frameworks 利用组氨酸-镍配位在金属-有机框架上稳定的酶固定

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-12-14 DOI: 10.1016/j.bej.2025.110050

Chenyang Huang , Wanning Gao , Mengting Chang , Xing Zhang , Junhua Tao , Yamei Lin , Lei Lin

Metal-organic frameworks (MOFs) have been widely explored as carriers for enzyme immobilization. However, conventional embedding and adsorption methods often suffer from limitations such as weak binding or enzyme leaching. In this study, we presented an enhanced immobilization strategy leveraging the chelation between histidine residues and nickel ions on Ni-based MOF, and we further validated the mechanism of enzyme immobilization through a histidine alkylation substitution strategy. This approach ensured strong enzyme anchoring on the MOF surface while providing superior protection to the enzyme, especially for the fragile glycoenzymes. Molecular dynamics (MD) simulations confirmed that not only the enzyme attachment was a rapid process, but also the structural integrity and catalytic activity of PmHS2 were preserved upon immobilization. The corresponding Enzyme-MOF system demonstrated remarkable stability, retaining 80.65 % activity after 7 recycling cycles and 80.95 % activity after 40 days of storage. These results confirmed that histidine-Ni²⁺ coordination is a highly effective strategy for improving enzyme immobilization performance.

金属有机骨架作为固定化酶的载体已被广泛探索。然而，传统的包埋和吸附方法往往受到弱结合或酶浸等限制。在本研究中，我们提出了一种利用组氨酸残基与镍离子在ni基MOF上螯合的强化固定化策略，并通过组氨酸烷基化取代策略进一步验证了酶固定化机制。这种方法确保了酶在MOF表面的强锚定，同时为酶提供了优越的保护，特别是对脆弱的糖酶。分子动力学（MD）模拟证实了PmHS2不仅是一个快速的酶附着过程，而且在固定后保持了PmHS2的结构完整性和催化活性。相应的酶- mof体系表现出显著的稳定性，在7次循环后保持80.65 %的活性，在储存40天后保持80.95 %的活性。这些结果证实了组氨酸- ni 2 +配合是提高酶固定化性能的一种非常有效的策略。

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引用次数: 0

Hybrid section-based modeling of gas-phase hydrodynamics in biotrickling filters: Influence of packing material on residence time distributions 基于混合截面的生物滴滤气相流体动力学建模：填料对停留时间分布的影响

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-12-13 DOI: 10.1016/j.bej.2025.110049

Felipe A. Carreño-López , Patricio A. Moreno-Casas , Felipe Scott , Alberto Vergara-Fernández , José Sierra-Pallares , Raúl Muñoz

In this study, the gas-phase hydrodynamics within a 6.5 L biotrickling filter were quantified using pulse-injection residence time distributions (RTDs) recorded with a cost-effective metal oxide (MOx) sensor. This was conducted at a constant gas flow rate of 8 L min⁻¹ across trickling liquid velocities (TLV) ranging from 0 to 10

{m h}^{- 1}

, using three different packings: polyurethane foam, Tri-Packs Jaeger spheres, and wood bark. A hybrid section-based model was developed and evaluated against two closed-closed axial dispersion variants (AD

_{c c}

+CSTR and AD

_{c c}

+PFR). Model discrimination using AIC/BIC and

R^{2}

indicated that the optimal model description is contingent upon the packing material and operating regime: the hybrid model most accurately represented foam and most wood cases, whereas AD

_{c c}

+CSTR was sufficient for spheres under irrigation, and AD

_{c c}

+PFR consistently underperformed. Employing the optimal model in each scenario, the Peclet number increased with TLV for PUF and spheres (with diminishing returns at the highest TLV), whereas wood exhibited a low Peclet number and non-monotonic behavior. By explicitly partitioning the mean residence time between the buffer zones and the bed, the hybrid model effectively separates the boundary hold-up from the core dispersion, yielding a more precise representation of the packed bed Peclet number. Overall, MOx-based RTD measurements, in conjunction with model comparison, offer a cost-effective in situ diagnostic tool for differentiating boundary mixing from bed transport, thereby informing packing selection and operating parameters in gas-phase biofiltration.

在这项研究中，利用脉冲注射停留时间分布（rtd）记录了一个具有成本效益的金属氧化物（MOx）传感器，定量了6.5 L生物滴滤器内的气相流体动力学。实验采用了三种不同的填料：聚氨酯泡沫、trii - packs Jaeger球体和木皮，在8 L min - 1的恒定气体流速下进行，滴液速度（TLV）范围为0到10 m h - 1。建立了一种基于切片的混合模型，并对两种闭式轴向弥散变量（ADcc+CSTR和ADcc+PFR）进行了评估。使用AIC/BIC和R2的模型判别表明，最佳模型描述取决于包装材料和操作制度：混合模型最准确地代表泡沫和大多数木材情况，而ADcc+CSTR对于灌溉下的球体足够，而ADcc+PFR一直表现不佳。采用最优模型，PUF和球体的Peclet数随TLV的增加而增加（在最高TLV时收益递减），而木材则表现出低Peclet数和非单调行为。通过显式划分缓冲区和床层之间的平均停留时间，混合模型有效地将边界占用率与核心色散分离开来，从而得到更精确的填充床佩莱特数表示。总的来说，基于mox的RTD测量，结合模型比较，提供了一种具有成本效益的现场诊断工具，用于区分边界混合和床层输送，从而为气相生物过滤的填料选择和操作参数提供信息。

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引用次数: 0

Microbial synthesis of L-fucose from glycerol and glucose in engineered Escherichia coli 用甘油和葡萄糖在工程大肠杆菌中合成L-聚焦物

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-12-13 DOI: 10.1016/j.bej.2025.110044

Yongwei Zhang , Jun Yang , Ying Lin , Qi Wang , Shuli Liang

L-Fucose is attracting more attention currently due to its multiple physiological and pharmaceutical functions via fucosylation forms in human body. However, studies regarding the biosynthesis of L-fucose were raised in just recent years, meanwhile the fine rewire of carbon flux in the microbial cell factory is the key for de novo biosynthesis and needs to be further investigated. Herein, we constructed a recombinant strain FZWP03 derived from Escherichia coli MG1655 for production of L-fucose through a combined strategy of genomic integration and plasmid-based expression of pathway genes, in addition to knockout of competing pathway. FZWP03 synthesized 2.68 and 0.96 g/L of L-fucose by shake-flask cultivation from glycerol and glucose, respectively. Then, a pfkA turn-off dynamic switch combining growth phase-dependent promoters (GPPs) and degrons was applied to dynamically fine-tune the carbon flux from fructose-6-phosphate (F6P) to GDP-L-fucose or EMP pathway, and the resulting strain FZWP06 exhibited a significant increase in L-fucose production. Furthermore, the native cryptic plasmids from E. coli Nissle 1917 were applied to construct FZWP08, enabling plasmid stabilization without antibiotic addition in fermentation processes. Finally, with glycerol or glucose as the de novo synthetic substrates, FZWP03, FZWP06, and FZWP08 achieved 21.38, 5.05, and 5.95 g/L L-fucose in fed-batch fermentation, respectively. The technical approaches raised in this study could be applied in the biosynthesis of other compounds.

L-聚焦蛋白在人体中通过聚焦化形式具有多种生理和药物功能，近年来受到越来越多的关注。然而，关于L- focus生物合成的研究是近年来才兴起的，而微生物细胞工厂中碳通量的精细重丝是从头合成的关键，需要进一步研究。本研究在敲除竞争途径的基础上，通过基因组整合和途径基因质粒表达相结合的策略，构建了源自大肠杆菌MG1655的重组菌株FZWP03，用于生产L- focus。FZWP03通过摇瓶培养分别以甘油和葡萄糖为原料合成了2.68和0.96 g/L的L- focus。然后，利用结合生长相依赖启动子（GPPs）和降解子（degrons）的pfkA关闭动态开关，对果糖-6-磷酸（F6P）向GDP-L-聚焦或EMP途径的碳通量进行动态微调，得到的菌株FZWP06的L-聚焦产量显著增加。此外，利用大肠杆菌Nissle 1917的原生隐质粒构建FZWP08，使质粒在发酵过程中无需添加抗生素即可稳定。最后，以甘油或葡萄糖作为新合成底物，FZWP03、FZWP06和FZWP08在补料分批发酵中分别获得21.38、5.05和5.95 g/L的L-聚焦。本研究提出的技术方法可应用于其他化合物的生物合成。

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引用次数: 0

Improvement of furfural tolerance and butanol production in Clostridium beijerinckii ART44 through overexpression of Class I heat shock proteins 通过过表达I类热休克蛋白提高贝氏梭菌ART44的糠醛耐受性和丁醇产量

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-12-11 DOI: 10.1016/j.bej.2025.110047

Chaoyue Shi , Caolin Liu , Xin Cheng, Yujie Jing, Qinghua Zhang, Wenjie Lai, Hanguang Li

The conversion of lignocellulosic hydrolysate into butanol offers a significant approach to reduce environmental pollution and the greenhouse effect. However, the presence of inhibitors in the hydrolysate restricts its utilization efficiency. In this study, we investigated the role of Class I heat shock proteins (HSPs) in Clostridium beijerinckii ART44 under furfural stress. Notably, the grpE gene was identified as being significantly upregulated under this condition. Therefore, to enhance the furfural tolerance and butanol production, the grpE gene was overexpressed. With this effort, the resulting recombinant strain, named as ART44/grpE, exhibited a 73.75 % increase in butanol production (5.34 ± 0.29 g/L) and a 53.99 % increase in total solvent production (7.67 ± 0.16 g/L) under 3.0 g/L furfural stress, compared to the wild-type strain ART44. Furthermore, mechanistic studies revealed that grpE overexpression reduced cell surface hydrophobicity, minimized the leakage of intracellular macromolecules, and increased the proportion of saturated fatty acids in the cell membrane. These alterations contributed to enhancing cell viability, improving butanol biosynthesis enzyme activity, and reducing membrane permeability under furfural stress. Collectively, the results demonstrate that overexpressing grpE in C. beijerinckii ART44 is an effective and attainable approach to obtain a high furfural tolerance strain, and then improving its butanol production from lignocellulose hydrolysate.

木质纤维素水解产物转化为丁醇为减少环境污染和温室效应提供了一条重要途径。然而，水解产物中抑制剂的存在限制了其利用效率。在本研究中，我们研究了一类热休克蛋白（HSPs）在糠醛胁迫下在贝氏梭菌ART44中的作用。值得注意的是，在这种情况下，grpE基因被鉴定为显著上调。因此，为了提高糠醛耐受性和丁醇产量，grpE基因被过表达。这个努力,由此产生的重组菌株,命名为ART44 / grpE,表现出73.75 %提高丁醇生产(5.34 ±0.29  g / L)和53.99 %增加溶剂总产量(7.67 ±0.16  g / L)低于3.0 g / L糠醛压力,与野生型菌株ART44相比。此外，机制研究表明，grpE过表达降低了细胞表面疏水性，减少了细胞内大分子的渗漏，增加了细胞膜中饱和脂肪酸的比例。这些变化有助于提高细胞活力，提高丁醇生物合成酶活性，降低糠醛胁迫下的膜通透性。综上所述，在C. beijerinckii ART44中过表达grpE是获得高糠醛耐受性菌株，进而提高其木质纤维素水解丁醇产量的有效途径。

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引用次数: 0

Nitrifier released extracellular organics: Characterization and their ecological role in synergy with heterotrophs 硝化物释放胞外有机物：表征及其与异养生物协同作用的生态作用

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-12-11 DOI: 10.1016/j.bej.2025.110046

Chenchen Yu, Yuyi Zheng, Weitie Lin, Jianfei Luo

Nitrifiers including ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB) are well-known to release dissolved organic carbon (DOC) and synthesize extracellular polymeric substances (EPS), while the composition and difference of these products between them remain unclear. Moreover, the ecological role of this trait in nitrifier during their synergy with heterotroph is still largely unknown. In this study, both strains of AOB and NOB were found to produce significant amounts of DOC (a maximum of 5.22 and 8.91 mg/L) and EPS (a maximum of 11.39 and 4.63 mg/L). The DOC composition was similar between the tested strains of AOB and NOB. The EPS synthesis was found to occur throughout the whole growth phases, but their composition between AOB and NOB was different. The nitrifier released DOC supported the growth of heterotrophs (with the maximum cell number reached 5.0 × 10⁷ CFU/mL), which in turn significantly promoted their growths (a maximum of 2.17 and 1.98 times higher), substrate oxidation activities and EPS syntheses. Co-cultivation with heterotroph significantly upregulated the functional genes of nitrifier that involving in substrate oxidation, CO₂ fixation, and EPS synthesis. The nitrifier-heterotroph synergy enhanced the nitrifiers’ substrate oxidation, indicating its significant ecological role in promoting nutrient removal in wastewater treatment.

氨氧化菌（AOB）和亚硝酸盐氧化菌（NOB）等硝化菌释放溶解有机碳（DOC）和合成胞外聚合物（EPS）是众所周知的，但这些产物的组成和差异尚不清楚。此外，该性状在硝化菌与异养菌协同作用中的生态作用在很大程度上仍是未知的。本研究发现，AOB和NOB菌株均能产生大量的DOC（最高5.22和8.91 mg/L）和EPS（最高11.39和4.63 mg/L）。AOB和NOB菌株的DOC组成相似。EPS的合成贯穿于整个生长阶段，但其组成在AOB和NOB之间存在差异。硝化物释放的DOC支持异养生物的生长（最大细胞数达到5.0 × 107 CFU/mL），从而显著促进异养生物的生长（最大细胞数提高2.17倍和1.98倍）、底物氧化活性和EPS合成。与异养菌共培养显著上调了涉及底物氧化、CO2固定和EPS合成的硝化菌功能基因。硝化菌与异养菌的协同作用增强了硝化菌对底物的氧化作用，表明其在废水处理中具有促进营养物去除的重要生态作用。

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引用次数: 0

Insights into microbial and sorptive regulation of chlorpyrifos-bispyribac dissipation in floating treatment wetlands 漂浮处理湿地中毒死蜱-双吡虫蜱耗散的微生物和吸附调控研究

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2025-12-10 DOI: 10.1016/j.bej.2025.110045

Kashaf Farooq , Muhammad Arslan , Samina Anwar , Maryam Noor , Muhammad Afzal

Agricultural runoff remains one of the most persistent threats to water quality worldwide. Floating wetlands (FWs), when designed appropriately, offer a promising nature-based solution. Here, we show how biologically and sorptively active FWs can remove two agrochemicals—chlorpyrifos (CPF) and bispyribac‑sodium (BIS), under response surface methodology (RSM)‑backed optimal operation (pH 8; 35 °C; 10 mg L⁻¹ each; 1 % glucose). FWs built with Phragmites australis (common reed) were amended with a defined consortium (CB2H, 1 % v/v), plant‑derived biochar (1.5 % w/v), biochar‑immobilized CB2H (1.5 % w/v), and nutrients (N 25 mg L⁻¹, P 25 mg L⁻¹, K 20 mg L⁻¹). CPF and BIS declined exponentially, fitting pseudo‑first‑order kinetics with adsorption component (high S, K_d in the immobilized system). CPF disappeared fastest in the consortium‑only and biochar‑immobilized treatments (k = 0.07 and 0.09 day⁻¹), resulting in > 99 % removal (>9.9 mg L⁻¹) by day 20; BIS peaked at > 84 % (8.4 mg L⁻¹) with immobilized cells. FTIR shifts (∼2920–2840, 2800 cm⁻¹) and new CO bands (1600–1800 cm⁻¹) indicated hydrogen bonding in the Phragmites biochar. Approximately 40 % of the CPF loss in the controls was abiotic (sorption/photolysis/hydrolysis). Chemical oxygen demand and ancillary pollutants also declined. Enhanced performance was supported by microbial colonization within biochar pores. The study provides key design constants (k, K_d), positioning engineered FWs as a scalable nature-based technology for pesticide-laden agricultural runoff.

农业径流仍然是全球水质最持久的威胁之一。如果设计得当，浮动湿地（FWs）是一种很有前途的基于自然的解决方案。在这里，我们展示了生物和吸附活性的FWs如何在响应面法（RSM）支持的最佳操作下（pH值8；35°C； 10 mg L⁻¹各；1 %葡萄糖）去除两种农药——毒死蜱（CPF）和双嘧菌酯钠（BIS）。美国鱼类和野生动物管理局用芦苇南极光(芦苇)修改与定义财团(CB2H 1 % v / v),植物-衍生生物炭(1.5 % w / v),生物炭-固定化CB2H(1.5 % w / v)和养分(N 25 mg  L⁻¹,P 25 毫克 L⁻¹,K 20 mg  L⁻¹)。CPF和BIS呈指数级下降，拟合吸附组分的准一级动力学（固定体系中S、Kd较高）。在纯联合治疗和生物炭固定治疗中，CPF消失得最快（k = 0.07和0.09天⁻¹），到第20天，CPF就被清除了>； 99% % （>9.9 mg L⁻¹）；在固定细胞时，BIS的峰值为>； 84 %（8.4 mg L⁻¹）。FTIR变化（~ 2920 - 2840,2800 cm⁻¹）和新的CO波段（1600-1800 cm⁻¹）表明芦苇生物炭中存在氢键。在对照组中，大约40% %的CPF损失是非生物的（吸收/光解/水解）。化学需氧量和辅助污染物也有所下降。生物炭孔隙内的微生物定殖支持了性能的提高。该研究提供了关键的设计常数（k, Kd），将工程FWs定位为一种可扩展的基于自然的农药农业径流技术。

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引用次数: 0