Biochemical Engineering Journal最新文献_第3页

Effectiveness of Musa balbisiana bract toward chromium removal from industrial tannery effluent: Optimization, kinetics, isotherms, regeneration, and cost estimation 苞片麝香草从工业制革废水中去除铬的效果：优化、动力学、等温线、再生和成本估算

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2024-11-12 DOI: 10.1016/j.bej.2024.109565

Divya Baskaran , Becky Miriyam I , Palani R , Hun-Soo Byun

Eradicating chromium from industrial effluent is essential for environmental security and economic reasons. This study investigates the potency of activated Musa balbisiana bract biomass as a biosorbent to remove hexavalent chromium (Cr⁶⁺) from real industrial tannery effluent (ITE). The characterization study exemplifies the existence of irregular structures and excessive cavities, and the occurrence of functional groups (hydroxyl, carboxyl, esters, and alkynes) are benefitting the deposition of Cr⁶⁺ on the biosorbent. A maximum biosorption capacity of 42.75 ± 0.21 mg/g was observed at an optimum pH of 6.5, biosorbent dosage of 0.3 g, initial Cr⁶⁺ concentration of 50 mg/L, and contact time of 120 min. The validation experiment confirmed the removal efficiency of total chromium, trivalent chromium, and Cr⁶⁺ 92.56 ± 0.80 %, 98.63 ± 0.20 %, and 96.21 ± 0.50 %, respectively. Among the models, Langmuir isotherm (R²: 0.9992) and pseudo-second order (R²: 0.9999) kinetic models greatly correlate with the equilibrium data. A 2-tier membrane module was examined for continuous study and reached 88.23 ± 0.60 % Cr⁶⁺ removal. Statistical analysis was performed to confirm the significance of adsorption results. The likelihood of the desorption and regeneration of the treated biosorbent was investigated. The estimated cost per volume of ITE treated and unit of pollutant removal from ITE employing Musa balbisiana bract biosorbent is around $3.08/m³ and $3.75/kg.

消除工业废水中的铬对环境安全和经济发展至关重要。本研究调查了活性苞片麝香草生物质作为生物吸附剂去除实际工业制革废水（ITE）中六价铬（Cr6+）的有效性。表征研究表明，不规则结构和过多空腔的存在以及官能团（羟基、羧基、酯和炔基）的出现有利于 Cr6+ 在生物吸附剂上的沉积。在最佳 pH 值为 6.5、生物吸附剂用量为 0.3 克、初始 Cr6+ 浓度为 50 毫克/升、接触时间为 120 分钟时，观察到最大生物吸附容量为 42.75 ± 0.21 毫克/克。验证实验证实，总铬、三价铬和 Cr6+ 的去除率分别为 92.56 ± 0.80 %、98.63 ± 0.20 % 和 96.21 ± 0.50 %。在这些模型中，Langmuir 等温线模型（R2：0.9992）和伪二阶动力学模型（R2：0.9999）与平衡数据有很大的相关性。对 2 层膜组件进行了连续研究，其对 Cr6+ 的去除率达到了 88.23 ± 0.60%。统计分析证实了吸附结果的重要性。研究了经处理的生物吸附剂解吸和再生的可能性。使用苞片麝香草生物吸附剂处理 ITE 的单位体积和去除 ITE 中污染物的单位成本估计分别约为 3.08 美元/立方米和 3.75 美元/千克。

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引用次数: 0

Simultaneous endogenous partial denitrification/anammox process for low-strength wastewater treatment: Process optimization, nitrogen removal and microbial dynamics 用于低浓度废水处理的同步内源部分反硝化/氨氧化工艺：工艺优化、脱氮和微生物动力学

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2024-11-09 DOI: 10.1016/j.bej.2024.109568

Dong Li , Fanxu Zeng , Songwei Yang , Yuliang Zhu , Zhu Li , Huiping Zeng , Jie Zhang

The endogenous partial denitrification process (EPD) led by glycogen-accumulating organisms (GAOs) has become an alternative to NO₂⁻ supply in mainstream anaerobic-ammonia oxidation (anammox). However, low autotrophic nitrogen removal contribution is an urgent problem that needs to be solved in simultaneous endogenous partial denitrification/anammox (EPDA) system. This study used anaerobic duration optimization to enhance the autotrophic nitrogen removal capacity of EPDA system. The results showed that the EPDA activity increased from 0.67 to 1.09 mg N/g VSS·h after anaerobic time was extended to 120 min. This significantly improved the contribution of anammox to TIN removal, increasing from 23.5 % to 61.6 %. During the phase Ⅲ, Eff.TIN of 4.5±1.8 mg/L and NRE of 92.2 %±3.0 %. The enrichment of AnAOB (Candidatus Brocadia) and GAOs (Defluviicoccus) was responsible for maintaining the stability of the EPDA process. This study provides a feasible optimization strategy for improving the contribution of autotrophic nitrogen removal in the EPDA system.

由糖原累积生物（GAOs）主导的内源部分反硝化过程（EPD）已成为主流厌氧氨氧化（anammox）过程中氮氧化物供应的替代方式。然而，在同时进行的内源部分反硝化/氨氧化（EPDA）系统中，自养脱氮贡献率低是一个亟待解决的问题。本研究利用厌氧时间优化来提高 EPDA 系统的自养脱氮能力。结果表明，厌氧时间延长到 120 分钟后，EPDA 活性从 0.67 mg N/g VSS-h 提高到 1.09 mg N/g VSS-h。这大大提高了anammox对TIN去除的贡献率，从23.5%提高到61.6%。在第Ⅲ阶段，Eff.TIN 为 4.5±1.8 mg/L，NRE 为 92.2 %±3.0 %。AnAOB（Candidatus Brocadia）和 GAOs（Defluviicoccus）的富集是维持 EPDA 过程稳定的原因。这项研究为提高 EPDA 系统自养脱氮的贡献率提供了可行的优化策略。

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引用次数: 0

Biodegradation of deep eutectic solvent pre-treated natural rubber gloves by Klebsiella aerogenes: A sustainable approach to rubber waste management 产气克雷伯氏菌对深共晶溶剂预处理天然橡胶手套的生物降解：橡胶废物管理的可持续方法

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2024-11-08 DOI: 10.1016/j.bej.2024.109569

Harika Chittella , Li Wan Yoon , Suganti Ramarad , Zee-Wei Lai

This study investigates the potential of deep eutectic solvents (DES) to enhance the biodegradation of natural rubber gloves (NRG) by Klebsiella aerogenes. Choline chloride and urea (ChCl: urea) was the DES employed to pre-treat NRG at various temperatures (80°C to 140°C) and durations (0.5 h to 5 h). Pre-treated rubber (p-NRG) underwent significant physical and chemical changes, enhancing its biodegradability. Analytical techniques such as dry weight analysis, bacteria cell concentration, FTIR TGA, and SEM were used to characterize the pre-treated and biodegraded samples. The results have demonstrated a significant weight loss and structural modifications in p-NRG, with the highest degradation of 43 % observed at 140°C for 5 hours of pretreatment before biodegradation. Meanwhile, merely 17 % of weight loss was observed when pre-treatment was not employed. DES pre-treatment notably enhanced NRG biodegradability, achieving a 50.6 % weight loss when biodegradation was conducted at pH 7 and 35°C. The highest cell concentration, 0.75 g/L, was recorded in the second week of the biodegradation process. Results have indicated that the maximum protein concentration of 697.3 µg/ml, along with the highest enzyme activities for laccase and manganese peroxidase (MnP) at 0.46 ± 0.05 IU and 0.30 ± 0.05 IU respectively, were recorded in the second week of the biodegradation process. DES pre-treatment has significantly improved the biodegradability of NRG by Klebsiella aerogenes, offering a promising and eco-friendly solution for rubber waste management.

本研究探讨了深共晶溶剂（DES）在增强气肿克雷伯氏菌对天然橡胶手套（NRG）的生物降解方面的潜力。氯化胆碱和尿素（氯化胆碱：尿素）是用于预处理 NRG 的 DES，预处理温度（80°C 至 140°C）和持续时间（0.5 小时至 5 小时）各不相同。预处理后的橡胶（p-NRG）发生了显著的物理和化学变化，提高了其生物降解性。分析技术包括干重分析、细菌细胞浓度、傅立叶变换红外热像仪、热重分析和扫描电镜，用于描述预处理和生物降解样品的特性。结果表明，p-NRG 的重量明显减少，结构也发生了改变，在生物降解前，预处理温度为 140°C，时间为 5 小时，降解率最高，达到 43%。与此同时，在未采用预处理的情况下，仅观察到 17% 的重量损失。DES 预处理显著提高了 NRG 的生物降解性，在 pH 值为 7、温度为 35°C 的条件下进行生物降解时，重量损失率为 50.6%。在生物降解过程的第二周，细胞浓度最高，达到 0.75 克/升。结果表明，在生物降解过程的第二周，蛋白质浓度最高，为 697.3 µg/ml ，漆酶和锰过氧化物酶（MnP）的酶活性最高，分别为 0.46 ± 0.05 IU 和 0.30 ± 0.05 IU。DES 预处理大大提高了 NRG 在产气克雷伯氏菌作用下的生物降解能力，为橡胶废物管理提供了一种前景广阔的生态友好型解决方案。

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引用次数: 0

The impact of 3D tumor spheroid maturity on cell migration and invasion dynamics 三维肿瘤球体成熟度对细胞迁移和侵袭动力学的影响

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2024-11-08 DOI: 10.1016/j.bej.2024.109567

Lingke Feng , Rong Pan , Ke Ning , Wen Sun , Yirong Chen , Yuanyuan Xie , Mingzhu Wang , Yan Li , Ling Yu

Cell motility is crucial in cancer metastasis, and understanding its regulation in tumor cells is vital for developing anti-metastatic therapies. Traditional 2D cell culture assays provide insights into cell migration but fail to replicate the complex 3D architecture of tissues in vivo. 3D cell culture models like tumor spheroids have been applied for cell migration tests. This study investigates the role of spheroid maturity in tumor cell motility, hypothesizing that spheroid maturity mirrors physiological conditions in solid tumors. Human prostate (DU 145), breast (MCF-7), and murine breast (EMT-6) cancer cells were cultured into spheroids of varying time (3, 7, and 11 days). The migration and invasion of these spheroids were analyzed, revealing that 11-day-old DU 145 spheroids demonstrated the greatest horizontal migration, correlating with RNA-seq data showing increased cell adhesion, cytoskeleton dynamics, and motility pathways. Confocal microscopy and single-cell multimode analyzer indicated higher reactive oxygen species (ROS) levels in mature spheroids, potentially activating motility pathways. Additionally, DU 145 spheroids were treated with chemotherapy reagent Doxorubicin (DOX), and the results showed that spheroids culture for 7 and 11 days exhibited greater resistance to DOX compared to spheroids cultured for 3 days. These findings highlighted the importance of considering spheroid maturity in cancer research and drug development, emphasizing the need for systematic analysis of spheroid growth conditions to ensure reproducible and reliable experimental settings.

细胞运动是癌症转移的关键，了解肿瘤细胞中的细胞运动调控对于开发抗转移疗法至关重要。传统的二维细胞培养试验可深入了解细胞迁移，但无法复制体内组织的复杂三维结构。肿瘤球体等三维细胞培养模型已被用于细胞迁移测试。本研究探讨了球体成熟度在肿瘤细胞迁移中的作用，假设球体成熟度反映了实体瘤的生理状况。研究人员将人类前列腺癌（DU 145）、乳腺癌（MCF-7）和小鼠乳腺癌（EMT-6）细胞培养成不同时间（3、7 和 11 天）的球形。对这些球形细胞的迁移和侵袭进行了分析，结果表明，培养 11 天的 DU 145 球形细胞的水平迁移能力最强，这与 RNA-seq 数据显示的细胞粘附性、细胞骨架动力学和运动途径增加有关。共聚焦显微镜和单细胞多模分析仪显示，成熟球体内的活性氧（ROS）水平较高，可能会激活运动途径。此外，用化疗试剂多柔比星（Doxorubicin，DOX）处理 DU 145 球形体，结果显示与培养 3 天的球形体相比，培养 7 天和 11 天的球形体对 DOX 的耐受性更强。这些发现突出了在癌症研究和药物开发中考虑球体成熟度的重要性，强调了对球体生长条件进行系统分析的必要性，以确保实验设置的可重复性和可靠性。

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引用次数: 0

Advanced anaerobic digestion by co-immobilization of anaerobic microbes and conductive particles in hydrogel for enhanced methane production performance 通过将厌氧微生物和导电颗粒共同固定在水凝胶中实现高级厌氧消化，从而提高甲烷生产性能

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2024-11-08 DOI: 10.1016/j.bej.2024.109563

Stella Chan , Kento Nishi , Mitsuhiko Koyama , Tatsushi Matsuyama , Junichi Ida

Recent research has increasingly focused on the enhancement of anaerobic digestion (AD) through direct interspecies electron transfer (DIET) facilitated by conductive particles (CP). Although this approach can significantly accelerate the AD process, the contact efficiency between CPs and AD microbes is relatively low due to the flow of water in a dispersed condition, leading to possible DIET inefficiency. In this study, a unique approach involving the “co-immobilization” of anaerobic microbes and multi-walled carbon nanotubes (MWCNTs) as CP into a hydrogel matrix was developed to improve the AD process. The advantages of this method include improved contact efficiency between microbes and CPs for enhanced DIET, and increased CP retention within the reactor, thereby omitting the need to compensate for CP washout. The methane production rate for the co-immobilized hydrogel was 2.5-fold and 1.9-fold faster than that of the control (dispersed sludge) and conventional DIET (dispersed sludge with MWCNT addition), respectively. Microbial analysis indicated the enrichment of functional microbes such as Anaerolineacea, Sedimentibacteraceae, Rhodocyclaceae, and Methanothrichaceae, which could be involved in the DIET under co-immobilized conditions. These results demonstrate the potential of the proposed method for realizing an advanced continuous AD process through improved DIET.

最近的研究越来越关注通过导电颗粒（CP）促进种间直接电子传递（DIET）来提高厌氧消化（AD）的效果。虽然这种方法可以大大加快厌氧消化过程，但由于水流处于分散状态，CP 与厌氧消化微生物之间的接触效率相对较低，可能导致 DIET 效率低下。本研究开发了一种独特的方法，将厌氧微生物和作为 CP 的多壁碳纳米管（MWCNTs）"共同固定 "到水凝胶基质中，以改进厌氧消化工艺。这种方法的优点包括提高微生物与氯化石蜡之间的接触效率，从而增强 DIET，并增加氯化石蜡在反应器中的保留率，从而无需补偿氯化石蜡的冲刷。与对照组（分散污泥）和传统 DIET（添加了 MWCNT 的分散污泥）相比，共固定水凝胶的甲烷生产率分别提高了 2.5 倍和 1.9 倍。微生物分析表明，Anaerolineacea、Sedimentibacteraceae、Rhodocyclaceae 和 Methanothrichaceae 等功能微生物富集，它们可能参与共同固定条件下的 DIET。这些结果表明，所提出的方法具有通过改进 DIET 实现先进的连续厌氧消化（AD）工艺的潜力。

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引用次数: 0

Developing an off-site bicarbonation absorber system to promote microalgal fixation of CO2 in exhaust gas from biogas upgrading 开发异地碳酸氢盐吸收系统，促进微藻固定沼气提纯废气中的二氧化碳

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2024-11-08 DOI: 10.1016/j.bej.2024.109558

Yulun Wu , Zhao Li , Xin Wang , Zaiyin Yu , Weiguang Mao , Cai Cheng , Guanmou Che , Jun Cheng

In order to address the risk of explosion due to CH₄ from exhaust gas produced during biogas upgrading in closed carbon fixation systems employing photosynthetic microalgae, an off-site bicarbonation absorber system was developed to promote microalgal CO₂ fixation under atmospheric pressure. The abundant CO₂ in the biogas upgrading exhaust gas (≥90 vol% CO₂, ≤10 vol% CH₄) reacted with a Na₂CO₃ solution in the off-site bicarbonation absorber to produce NaHCO₃, which was used as carbon source for microalgal growth in enclosed column photobioreactors. After the reaction, CH₄ was discharged outside the bicarbonation absorber because it did not react with the Na₂CO₃ solution and was extremely difficult to dissolve in water, thereby avoiding the explosion risk due to accumulated CH₄ in the enclosed column photobioreactors. The experimental results showed that the Spirulina growth rate first increased 1.7 times, peaking at 0.6 g/L/d, and then decreased when the bicarbonation reaction time (optimal 50 min), absorber diameter (optimal 10 cm), initial Na₂CO₃ concentration (optimal 173 mM), and exhaust gas aeration rate (optimal 100 sccm) increased. The optimal molar ratio of NaHCO₃ to total inorganic carbon in the bicarbonation absorber solution reached 79 %. The sufficient HCO₃^- supply and suitable pH of the microalgal solution improved the synthesis of photosynthetic pigments in the microalgal cells and enhanced their photochemical efficiency and carbon sequestration rates.

为了解决采用光合作用微藻的封闭式碳固定系统在沼气升级过程中产生的废气中的 CH4 导致爆炸的风险，开发了一种异地碳酸氢盐吸收系统，以促进微藻在大气压力下固定 CO2。沼气提纯废气中丰富的 CO2（≥90 vol% CO2，≤10 vol% CH4）与异位重碳酸盐吸收器中的 Na2CO3 溶液反应生成 NaHCO3，NaHCO3 用作封闭柱状光生物反应器中微藻生长的碳源。反应结束后，由于 CH4 不会与 Na2CO3 溶液发生反应，且极难溶解于水，因此 CH4 被排放到了碳酸氢盐吸收器之外，从而避免了封闭式柱状光生物反应器中累积的 CH4 所带来的爆炸风险。实验结果表明，当重碳酸化反应时间（最佳值为 50 分钟）、吸收器直径（最佳值为 10 厘米）、初始 Na2CO3 浓度（最佳值为 173 mM）和废气通气速率（最佳值为 100 sccm）增加时，螺旋藻的生长速率先增加 1.7 倍，达到 0.6 g/L/d 的峰值，然后下降。双碳酸化吸收器溶液中 NaHCO3 与总无机碳的最佳摩尔比达到 79%。微藻溶液中充足的 HCO3- 供应和适宜的 pH 值改善了微藻细胞中光合色素的合成，提高了其光化学效率和固碳率。

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引用次数: 0

A novel tubular single-chamber microbial electrolysis cell for efficient methane production from industrial potato starch wastewater 从工业马铃薯淀粉废水中高效生产甲烷的新型管式单室微生物电解池

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2024-11-07 DOI: 10.1016/j.bej.2024.109561

Gaoyuang Shang , Jinpeng Yu , Kai Cui , Hong Zhang , Yuhan Guo , Menglong Zhao , Chengjun Wang , Kun Guo

The integration of microbial electrolysis cells (MEC) with anaerobic digestion (AD) shows great promise for enhancing methane production from high-COD wastewater. However, an efficient MEC-AD reactor design remains elusive. Here, a novel tubular single-chamber MEC-AD reactor was constructed to treat potato starch wastewater (COD over 20,000 mg/L). The concentric and compact design of the stainless-steel cathode and anode reduced internal resistance, resulting in enhanced methane production. Applying −0.2 V vs. Ag/AgCl to the anode increased methane production by 1.73 times compared to the open circuit and halved hydraulic retention time. Moreover, the reactor achieved an average methane content of 82.57 %, which was 23.89 % higher than the open circuit. The reactor showed a total COD removal of 92.2 %, which was 24 % higher than the open circuit. Additionally, base consumption to maintain pH was reduced to one-sixth of that in conventional AD, preventing volatile fatty acid accumulation. Microbial analysis showed Geobacter (63.4 %) and Methanobacterium (96.8 %) were highly enriched in the anode and cathode biofilms, respectively. The proportion of fermentative bacteria also increased in the MEC-AD system. These results demonstrate the effectiveness of the tubular single-chamber MEC-AD reactor in enhancing methane production from potato starch wastewater, with strong potential for scale-up applications.

微生物电解池（MEC）与厌氧消化（AD）的结合为提高高 COD 废水的甲烷产量带来了巨大希望。然而，高效的 MEC-AD 反应器设计仍然遥遥无期。在此，我们建造了一个新型管式单室 MEC-AD 反应器来处理马铃薯淀粉废水（COD 超过 20,000 mg/L）。不锈钢阴极和阳极的同心紧凑设计降低了内阻，从而提高了甲烷产量。在阳极上施加 -0.2 V 对 Ag/AgCl 的电压，甲烷产量比开式回路增加了 1.73 倍，水力停留时间缩短了一半。此外，反应器的平均甲烷含量达到 82.57%，比开式回路高出 23.89%。反应器的化学需氧量总去除率为 92.2%，比开式回路高 24%。此外，维持 pH 值所需的碱消耗量减少到传统厌氧消化法的六分之一，从而防止了挥发性脂肪酸的积累。微生物分析表明，阳极生物膜和阴极生物膜中分别高度富集了 Geobacter（63.4%）和 Methanobacterium（96.8%）。在 MEC-AD 系统中，发酵菌的比例也有所增加。这些结果证明了管式单室 MEC-AD 反应器在提高马铃薯淀粉废水甲烷产量方面的有效性，具有很强的放大应用潜力。

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引用次数: 0

Improvement of sludge dewatering performance by persulfate advanced oxidation combined with LDH: Synergistic effect of free radical and non-free radical and reuse of deep-dewatered sludge cake 过硫酸盐高级氧化结合 LDH 改善污泥脱水性能：自由基和非自由基的协同效应以及深度脱水污泥饼的再利用

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2024-11-07 DOI: 10.1016/j.bej.2024.109560

Jingyu Lv, Lei Song, Yang Guo

The high-water content of sludge in wastewater plant will influence the transportation and utilization. In this study, a new method for improving sludge dewatering by pyrite (FeS₂) activated persulfate (PMS) combined with layered double hydroxide (LDH) was proposed. After conditioning, the water content (Wc) and specific resistance (SRF) of sludge decreased from 97.12 % and 1.83 × 10¹³ m/kg to 71.39 % and 1.84 × 10¹² m/kg, severally. SEM and particle size analysis showed the system could destroy sludge cells effectively.The mechanism analysis of protein and polysaccharide content, 3D-EEM, FTIR, XPS results showed that FeS₂/PMS-LDH combined system was beneficial to break down the sludge extracellular polymer (EPS), transform and accumulate the organic matter into the EPS outer layer, release the bound water. Both free radical and non-free radical play a role in oxidation, and they cooperate to break EPS. The effective phosphate adsorption performance of the biochar adsorbent prepared from dehydrated sludge cake was also investigated. The adsorption behavior of phosphate on biochar from dewatered sludge cake belongs to uniform chemical monolayer adsorption. When T = 298k, PH = 5, the maximum adsorption capacity is 20.255 mg/g. The introduction of LDH is helpful to enhance the sludge dewatering and the adsorption of phosphate. To sum up, the combined conditioning method considers the effectiveness of sludge dewatering and the feasibility of sludge cake disposal and utilization.

污水厂污泥含水率高，会影响污泥的运输和利用。本研究提出了一种利用黄铁矿（FeS2）活化过硫酸盐（PMS）结合层状双氢氧化物（LDH）改善污泥脱水的新方法。经过调理后，污泥的含水率（Wc）和比阻力（SRF）分别从 97.12 % 和 1.83 × 1013 m/kg 降至 71.39 % 和 1.84 × 1012 m/kg。蛋白质和多糖含量的机理分析、3D-EEM、傅立叶变换红外光谱、XPS 结果表明，FeS2/PMS-LDH 组合系统有利于分解污泥胞外聚合物（EPS），将有机物转化并积聚到 EPS 外层，释放结合水。自由基和非自由基都在氧化过程中发挥作用，它们共同作用使 EPS 断裂。研究还考察了脱水污泥饼制备的生物炭吸附剂对磷酸盐的有效吸附性能。脱水污泥饼生物炭对磷酸盐的吸附行为属于均匀化学单层吸附。当 T = 298k，PH = 5 时，最大吸附容量为 20.255 mg/g。LDH的引入有助于提高污泥脱水和磷酸盐的吸附能力。总之，联合调理法既考虑了污泥脱水的有效性，又考虑了污泥饼处置和利用的可行性。

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引用次数: 0

Sustainable remediation of piggery wastewater using a novel mixotrophic Chlorella sorokiniana Cbeo for high value biomass production 利用新型混养小球藻 Cbeo 对养猪场废水进行可持续修复，以生产高价值生物质

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2024-11-06 DOI: 10.1016/j.bej.2024.109555

Do Thi Cam Van , Dang Thi Mai , Bui Thi Thu Uyen , Nguyen Thi Phuong Dung , Lu Thi Thu Ha , Nguyen Thi Lieu , Dang Nhat Minh , Tran Dang Thuan , Le Truong Giang

Piggery wastewater (PW) contains high density of organic carbon (COD), nitrogen (NH₄⁺-N and TN) and phosphorous (TP), which are essential nutrients for microalgae growth. This work was attempted to use a newly isolate Chlorella sorokiniana C_beo for recovery these compounds into its biomass via mixotrophic cultivation. Critical factors including level of ammonia, C/N ratio, pH, light intensity, sterilized/unsterilized media, and indoor/outdoor cultivations affecting biomass production and nutrients removal efficiencies were investigated. Data revealed that C. sorokiniana C_beo achieved the optimal growth in the unsterilized medium at NH₄⁺-N concentration, C/N ratio, initial pH, and light intensity of 250 mg/L, 10/1, 7, and 150 μmol/m²/s, respectively. Under the optimal conditions, dry cell weight (DCW) reached the maximal level of 4.30 g/L, which was slightly higher than 4.14 g/L determined for the sterilized medium. In 30 L-scale photobioreactor, C. sorokiniana C_beo grown under indoor and outdoor achieved DCW of 3.61 and 3.19 g/L, respectively. COD, NH₄⁺-N, TN, TP removal efficiencies for both conditions were determined as 91.9–96.7, 96.6–99.7, 96.2–96.4, and 98.2–100 %, respectively. The C. sorokiniana C_beo biomass contained 14–27 % lipid, 25–32 % carbohydrate, 44–48 % protein, and 0.25–0.97 % lutein. Interestingly, α-Linolenic acid (C18:3n3) was 19.84 –27.0 % of the total fatty acids. C. sorokiniana C_beo is the promising algal strain for development of a sustainable biorefinery of PW.

养猪场废水（PW）中含有高浓度的有机碳（COD）、氮（NH4+-N 和 TN）和磷（TP），这些都是微藻生长所必需的营养物质。本研究试图利用一种新分离的小球藻 Cbeo，通过混养培养将这些化合物回收到其生物量中。研究了影响生物量生产和营养物质去除效率的关键因素，包括氨水平、C/N 比、pH 值、光照强度、灭菌/未灭菌培养基以及室内/室外培养。数据显示，当 NH4+-N 浓度、C/N 比、初始 pH 值和光照强度分别为 250 mg/L、10/1、7 和 150 μmol/m2/s 时，C. sorokiniana Cbeo 在未灭菌培养基中达到最佳生长。在最佳条件下，干细胞重量（DCW）达到最大值 4.30 g/L，略高于灭菌培养基的 4.14 g/L。在 30 L 规模的光生物反应器中，在室内和室外条件下生长的 C. sorokiniana Cbeo 的干细胞重量分别为 3.61 和 3.19 g/L。两种条件下的 COD、NH4+-N、TN、TP 去除率分别为 91.9%-96.7%、96.6%-99.7%、96.2%-96.4% 和 98.2%-100%。C. sorokiniana Cbeo 的生物质含有 14-27 % 的脂质、25-32 % 的碳水化合物、44-48 % 的蛋白质和 0.25-0.97 % 的叶黄素。有趣的是，α-亚麻酸（C18:3n3）占脂肪酸总量的 19.84 -27.0%。C. sorokiniana Cbeo 是开发 PW 可持续生物精炼厂的理想藻种。

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引用次数: 0

Experimental determination of maximum shear stress in Mobius® Breez perfusion microbioreactors and comparative analysis with stirred tank bioreactors Mobius® Breez 灌注微型生物反应器中最大剪切应力的实验测定以及与搅拌罐生物反应器的比较分析

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2024-11-06 DOI: 10.1016/j.bej.2024.109556

Patrick Romann , Dan Trunov , Ondřej Šrom , Harry L.T. Lee , Kevin S. Lee , Ryan Trocki , David Ephraim , Jean-Marc Bielser , Jonathan Souquet , Miroslav Šoóš , Thomas K. Villiger

Perfusion processes have experienced increased popularity in recent years due to their ability to sustain high cell densities and productivities in biopharmaceutical production, offering advantages over traditional batch and fed-batch cultivation methods. The Mobius® Breez microbioreactor significantly reduces experimental effort by downsizing the classical volume of perfusion bioreactors to the mL range and thus represents a valuable tool for process development. However, miniaturization has raised questions regarding comparability with traditional bioreactors in terms of the physical environment, such as hydrodynamic shear stress. Therefore, the maximum hydrodynamic shear stress, cultivation performance, and membrane-wall contact were evaluated to elucidate the system's behavior. Findings reveal two distinct operational conditions, distinguished by the presence or absence of membrane-wall contact, resulting in varying levels of hydrodynamic stress. Conditions lacking membrane contact demonstrate stress levels within safe operating thresholds for CHO cells, while those involving membrane contact exceed these thresholds, potentially leading to cell damage. Through the identification of critical frequencies of membrane motion, this study offers insights for optimizing microbioreactor operation and enhancing overall bioprocess efficiency.

灌流工艺近年来越来越受欢迎，因为它能够在生物制药生产中维持较高的细胞密度和生产率，与传统的间歇式和喂料式培养方法相比更具优势。Mobius® Breez 微型生物反应器将传统灌流生物反应器的体积缩小到毫升范围，大大减少了实验工作量，因此是工艺开发的重要工具。然而，微型化在物理环境（如流体动力剪切应力）方面引起了与传统生物反应器可比性的问题。因此，我们对最大流体动力剪切应力、培养性能和膜壁接触进行了评估，以阐明该系统的行为。研究结果显示了两种不同的操作条件，以膜壁接触的有无来区分，从而产生不同程度的流体动力应力。没有膜接触的情况下，应力水平在 CHO 细胞的安全操作阈值范围内，而有膜接触的情况下，应力水平超过了这些阈值，有可能导致细胞损伤。通过确定膜运动的临界频率，这项研究为优化微生物反应器的运行和提高整体生物处理效率提供了启示。

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引用次数: 0