Biochemical Engineering Journal最新文献_第3页

CRISPR/Cas9-mediated knockout of DYRK1B in triple-negative breast cancer cells: implications for cell proliferation, apoptosis, and therapeutic sensitivity CRISPR/Cas9 介导的三阴性乳腺癌细胞 DYRK1B 基因敲除：对细胞增殖、凋亡和治疗敏感性的影响

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2024-10-29 DOI: 10.1016/j.bej.2024.109553

Asrin Rashidi , Ernst-Martin Füchtbauer , Zakaria Vahabzadeh , Farzad Soleimani , Karim Rahimi , Bahram Nikkhoo , Shohreh Fakhari , Mohammad Bagher Khadem Erfan , Asaad Azarnezhad , Arash Pooladi , Fariborz Soheili , Fardin Fathi

Breast cancer is the most common cancer among women worldwide, with the triple-negative subtype (TNBC) having a poor prognosis and limited treatment options. DYRK1B is a dual-specificity kinase that regulates the cell cycle and quiescence. While its role in several cancers has been characterized, its role in TNBC remains unknown. In this study, we used CRISPR/Cas9 to delete DYRK1B in MDA-MB-231 cells, a model of TNBC and investigated its effects on cell proliferation, apoptosis, invasion, migration, angiogenesis, and response to Paclitaxel. The DYRK1B knockout (KO) was confirmed by PCR, Real-time qPCR, and Sanger sequencing. KO cells showed a significant reduction in cell proliferation, colony formation, invasion, and migration. Additionally, there were alterations in mRNA expression levels of several genes related to the cell cycle, angiogenesis, and cell motility, such as CCND1, MCM2, PCNA, CDKN1B, HIF1A, VEGFA, and WASF3, compared to MDA-MB-231 wild type (WT) cells. Immunocytochemistry results assessing Ki67 expression, a marker of cell proliferation, indicated that DYRK1B knockout cells had significantly lower Ki67 expression than WT cells. Furthermore, KO cells exhibited increased apoptosis and sensitivity to contact inhibition. Additionally, the IC₅₀ for Paclitaxel was significantly decreased in KO cells. These results suggest that DYRK1B plays an important role in the survival and invasion of TNBC cells and might be a potential candidate as a new therapeutic target for this disease.

乳腺癌是全球妇女最常见的癌症，其中三阴性亚型（TNBC）预后较差，治疗方案有限。DYRK1B 是一种双重特异性激酶，可调节细胞周期和静止状态。虽然DYRK1B在多种癌症中的作用已被证实，但它在TNBC中的作用仍不清楚。在本研究中，我们使用 CRISPR/Cas9 技术在 TNBC 模型 MDA-MB-231 细胞中删除了 DYRK1B，并研究了它对细胞增殖、凋亡、侵袭、迁移、血管生成和对紫杉醇反应的影响。通过 PCR、实时 qPCR 和 Sanger 测序证实了 DYRK1B 基因敲除（KO）。KO细胞在细胞增殖、集落形成、侵袭和迁移方面均表现出明显的下降。此外，与 MDA-MB-231 野生型（WT）细胞相比，与细胞周期、血管生成和细胞运动相关的几个基因（如 CCND1、MCM2、PCNA、CDKN1B、HIF1A、VEGFA 和 WASF3）的 mRNA 表达水平也发生了变化。免疫细胞化学评估细胞增殖标志物 Ki67 表达的结果表明，DYRK1B 基因敲除细胞的 Ki67 表达明显低于 WT 细胞。此外，KO 细胞表现出更强的凋亡能力和对接触抑制的敏感性。此外，KO 细胞中紫杉醇的 IC50 值明显降低。这些结果表明，DYRK1B在TNBC细胞的存活和侵袭过程中起着重要作用，可能成为治疗这种疾病的新靶点。

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引用次数: 0

Cell Dome-based transfection array for non-adherent suspension cells 基于细胞穹顶的非粘附悬浮细胞转染阵列

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2024-10-29 DOI: 10.1016/j.bej.2024.109554

Ryotaro Kazama , Satoshi Fujita , Shinji Sakai

Cell-based microarrays are valuable tools for analyzing cellular functions. However, a significant limitation of conventional microarrays is their inapplicability to non-adherent cells. In this study, we investigated the potential of the ‘Cell Dome’ (diameter: 1 mm, height: approximately 300 µm) with a 90 µm-thick hydrogel shell as a gene transfection array for non-adherent cells in suspension. The human lymphoma cell line (K562 cells), used as a model for non-adherent cells, was transfected more efficiently by Lipofectamine/pDNA complexes on a composite hydrogel made of polyvinyl alcohol derivative (PVA-Ph) and chitosan derivative (chitosan-Ph) than hydrogels composed of an alginate derivative or PVA-Ph alone. Moreover, no significant adverse effects on the viability and proliferation of the enclosed cells were observed for Cell Dome with a PVA-Ph/chitosan-Ph composite hydrogel shell. Lipofectamine/pDNA complexes released from the bottom of Cell Domes could transfect the enclosed cells without leaking or contaminating adjacent Cell Domes. These results demonstrate the potential of Cell Domes with an appropriate hydrogel shell as transfection arrays for non-adherent cells in suspension, thereby expanding the range of applications of cell-based array technologies. This novel Cell-Dome transfection array would be a valuable tool for analyzing the cellular function of non-adherent cells in suspension and showcases the potential for providing important biomedical insights for future research and developments.

基于细胞的芯片是分析细胞功能的重要工具。然而，传统微阵列的一个重要局限是不适用于非粘附细胞。在这项研究中，我们研究了带有 90 微米厚水凝胶外壳的 "细胞穹顶"（直径：1 毫米，高度：约 300 微米）作为基因转染阵列用于悬浮非粘附细胞的潜力。以人类淋巴瘤细胞株（K562 细胞）为非粘附细胞模型，在由聚乙烯醇衍生物（PVA-Ph）和壳聚糖衍生物（壳聚糖-Ph）组成的复合水凝胶上，脂质体转染胺/pDNA 复合物的转染效率要高于单独由海藻酸衍生物或 PVA-Ph 组成的水凝胶。此外，带有 PVA-Ph/ 壳聚糖-Ph 复合水凝胶外壳的 Cell Dome 对被包裹细胞的活力和增殖没有明显的不良影响。从细胞穹顶底部释放的脂质体/pDNA 复合物可以转染被封闭的细胞，而不会泄漏或污染相邻的细胞穹顶。这些结果表明，带有适当水凝胶外壳的细胞穹顶具有作为悬浮非粘附细胞转染阵列的潜力，从而扩大了基于细胞的阵列技术的应用范围。这种新型细胞穹顶转染阵列将成为分析悬浮液中非粘附细胞功能的重要工具，并有可能为未来的研究和开发提供重要的生物医学见解。

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引用次数: 0

Removal of lysozyme as protein waste using weak ion exchange nanofiber membrane in a batch system: Linear and nonlinear model analysis 在批处理系统中使用弱离子交换纳米纤维膜去除作为蛋白质废物的溶菌酶：线性和非线性模型分析

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2024-10-29 DOI: 10.1016/j.bej.2024.109550

Dinh Thi Hong Thanh , Nguyen The Duc Hanh , Bing-Lan Liu , Penjit Srinophakun , Chen-Yaw Chiu , Shen-Long Tsai , Kuei-Hsiang Chen , Yu-Kaung Chang

This study investigates the removal of lysozyme, a common waste protein, using weak ion-exchange nanofiber membranes (P-COOH) to address challenges in protein waste management and wastewater treatment. Protein waste, prevalent in industrial and biological processes, contributes to environmental pollution and increases treatment complexity if not effectively managed. Conventional methods often face limitations in selectivity and efficiency, highlighting the need for innovative solutions. The P-COOH nanofiber membrane, with its high surface area and tailored functional groups, was engineered to optimize protein removal. The removal process was analyzed in a batch system at an optimal pH of 7. Various kinetic models, such as pseudo-first-order, pseudo-second-order, Avrami, and intra-particle diffusion, were applied to elucidate rate-controlling steps. In contrast, isotherm models, including Langmuir, Freundlich, and Temkin, characterized the equilibrium of the removal process. Complete elution of removed lysozyme was achieved using a 0.3 M NaCl solution, yielding an equilibrium binding capacity of 315.24 mg/g on the nanofiber membranes. These findings indicate that the Avrami nonlinear model best describes lysozyme removal's complex, multi-step kinetics. In contrast, the Langmuir linear model accurately fits the equilibrium data, suggesting monolayer removal on a homogeneous surface. The successful reusability of the membranes underscores their potential for sustainable lysozyme removal from wastewater, offering a viable solution for improved waste management in industrial applications.

本研究调查了使用弱离子交换纳米纤维膜（P-COOH）去除溶菌酶（一种常见的废弃蛋白质）的情况，以应对蛋白质废弃物管理和废水处理方面的挑战。蛋白质废物普遍存在于工业和生物过程中，如果得不到有效管理，会造成环境污染并增加处理的复杂性。传统方法往往在选择性和效率方面受到限制，因此需要创新的解决方案。P-COOH 纳米纤维膜具有高表面积和定制功能基团，可优化蛋白质去除效果。在最佳 pH 值为 7 的批处理系统中对去除过程进行了分析。各种动力学模型，如伪一阶、伪二阶、Avrami 和颗粒内扩散模型，被用于阐明速率控制步骤。相反，等温线模型，包括 Langmuir、Freundlich 和 Temkin，则描述了去除过程的平衡状态。使用 0.3 M NaCl 溶液可实现溶菌酶的完全洗脱，纳米纤维膜上的平衡结合能力为 315.24 mg/g。这些研究结果表明，阿夫拉米非线性模型最能描述溶菌酶去除的复杂、多步骤动力学。与此相反，Langmuir 线性模型精确地拟合了平衡数据，表明在均匀表面上的单层去除。膜的成功重复使用强调了其可持续去除废水中溶菌酶的潜力，为改善工业应用中的废物管理提供了可行的解决方案。

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引用次数: 0

Genosensor based on polypyrrole and dendrimer-coated gold nanoparticles for human papillomavirus detection 基于聚吡咯和树枝状聚合物包覆金纳米粒子的基因传感器，用于检测人类乳头瘤病毒

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2024-10-29 DOI: 10.1016/j.bej.2024.109551

Heloisa B. Dantas , Alberto G. Silva-Junior , Norma L.C.L. Silva , Abdelhamid Errachid , Maria D.L. Oliveira , Cesar A.S. Andrade

Human Papillomavirus (HPV) is an often asymptomatic widespread sexually transmitted infection responsible for causing various health issues. Low-risk HPV primarily causes genital warts. High-risk HPV types are associated with several cancers, including cervical, anal, and oropharyngeal cancers, posing significant health risks. In this work, we developed an electrochemical biosensor for the detection and differentiation of HPV genotypes based on electropolymerized polypyrrole (PPy) and PAMAM dendrimer-coated gold nanoparticles (PAMAM-AuNPs) for the immobilization of a DNA probe for detecting different HPV genotypes. Electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), and atomic force microscopy (AFM) were used to characterize the biosensor. AFM analysis revealed varied topographic surfaces associated with the increased peaks concerning the biosensor and against patient samples. Electrochemical responses indicated that the genosensor could detect HPV using plasmid (HPV 6, 16, 18, 31, and 33) and cDNA samples (HPV 6, 18, and 31) from infected patients. Different electrochemical profiles were obtained between high-risk and low-risk genotypes. The sensor presented an excellent analytical performance, presenting a lower LOD of 0.04 pg.µL⁻¹ and 0.34 pg.µL⁻¹ for plasmidial and cDNA samples, respectively. Electrochemical analysis pointed out the ability of the developed genosensor platform to differentiate the HPV genotypes. The proposed biosensor is a promising tool for detecting and monitoring HPV and related diseases such as cervical cancer.

人类乳头瘤病毒（HPV）是一种通常无症状的广泛性传播感染，可导致各种健康问题。低危型 HPV 主要导致生殖器疣。高危型 HPV 与多种癌症有关，包括宫颈癌、肛门癌和口咽癌，对健康构成重大威胁。在这项工作中，我们开发了一种用于检测和区分 HPV 基因型的电化学生物传感器，该传感器基于电聚合聚吡咯（PPy）和 PAMAM 树枝状聚合物包覆的金纳米粒子（PAMAM-AuNPs），用于固定 DNA 探针以检测不同的 HPV 基因型。电化学阻抗谱（EIS）、循环伏安法（CV）和原子力显微镜（AFM）被用来表征生物传感器。原子力显微镜分析表明，生物传感器和患者样本的峰值增加与不同的地形表面有关。电化学反应表明，该基因传感器可使用来自感染患者的质粒（HPV 6、16、18、31 和 33）和 cDNA 样品（HPV 6、18 和 31）检测 HPV。高危基因型和低危基因型的电化学特征各不相同。该传感器具有出色的分析性能，对质粒样品和 cDNA 样品的 LOD 分别为 0.04 pg.µL-1 和 0.34 pg.µL-1。电化学分析表明，所开发的基因传感器平台具有区分 HPV 基因型的能力。所提出的生物传感器是检测和监测人乳头瘤病毒及相关疾病（如宫颈癌）的一种有前途的工具。

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引用次数: 0

Self-healing of concrete crack based on modified zeolite immobilizing microorganisms 基于固定微生物的改性沸石的混凝土裂缝自愈合技术

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2024-10-28 DOI: 10.1016/j.bej.2024.109541

Nansha Ye , Zhen Liu , Peng Wang , Yongshuai Sun , Xiangli He

Selecting the appropriate carrier to safeguard the microorganisms and enhance their viability within the matrix while implementing self-healing in concrete by microbial induced calcium carbonate precipitation technology is challenging. In this study, modified zeolite was used as the microbial carrier for concrete. Varying concentrations of acid and alkali reagents were used to impregnate natural zeolite, and the optimal modification reagent was identified. The mechanical compatibility of zeolite and concrete was analyzed. The mineralization efficacy of microorganisms was assessed by analyzing the self-healing indices of concrete crack specimens, the optimal dosage of modified zeolite was determined. And the mechanism of this system was explored. The results indicate that when the bacterial solution concentration is 3×10⁷ cfu/mL, incorporating modified zeolite (particle size: 1.0–2.0 mm) with a 0.4 mol/L HCl solution yields the optimal microbial immobilization and mineralization effects. Also, with equivalent zeolite dosage, modified zeolite self-healing concrete can enhance compressive strength recovery by 8–22 % and reduce water absorption by 11–20 %, making it capable of completely repairing wider cracks. Concrete specimens with 30 % and 40 % modified zeolite content demonstrated excellent performance, with the 40 % dosage exhibiting the highest self-healing capability. At the 40 % modified zeolite dosage, concrete achieved a remarkable 86.8 % compressive strength recovery at 28 days and a 5.4 % water absorption rate, effectively repairing cracks up to 0.5 mm width.

通过微生物诱导碳酸钙沉淀技术实现混凝土自愈合的同时，选择合适的载体来保护微生物并提高其在基质中的存活率是一项挑战。在这项研究中，改性沸石被用作混凝土的微生物载体。使用不同浓度的酸碱试剂浸渍天然沸石，并确定了最佳改性试剂。分析了沸石与混凝土的机械相容性。通过分析混凝土裂缝试件的自愈合指数，评估了微生物的矿化功效，确定了改性沸石的最佳用量。并探讨了该系统的机理。结果表明，当细菌溶液浓度为 3×107 cfu/mL 时，在 0.4 mol/L HCl 溶液中加入改性沸石（粒径：1.0-2.0 mm）可获得最佳的微生物固定和矿化效果。此外，在沸石用量相等的情况下，改性沸石自修复混凝土的抗压强度恢复能力可提高 8-22%，吸水率降低 11-20%，使其能够完全修复较宽的裂缝。改性沸石含量分别为 30% 和 40% 的混凝土试样表现出优异的性能，其中 40% 的改性沸石含量表现出最高的自愈合能力。在改性沸石掺量为 40% 的情况下，混凝土在 28 天时的抗压强度恢复率高达 86.8%，吸水率为 5.4%，可有效修复宽度达 0.5 毫米的裂缝。

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引用次数: 0

CFD-guided scaling of Pseudomonas putida fermentation 以 CFD 为指导的假单胞菌发酵规模化

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2024-10-28 DOI: 10.1016/j.bej.2024.109549

Maryam Jamshidzadeh , Antonia Ursula Griesz , Jesper Wang Jensen , Ulrich Krühne , John M. Woodley , Krist V. Gernaey , Pablo Ivan Nikel , Helena Junicke

This study evaluated the scale-up of Pseudomonas putida fed-batch fermentation from a 2 L benchtop-scale stirred bioreactor to a 200 L pilot-scale tank by using a validated computational fluid dynamic (CFD) model. One of the major concerns in this fermentation process is the potential reduction in mixing quality with increasing scale, leading to lower yield or product quality. For a low-risk scale-up, a multiphase Euler-Euler CFD model was developed that simulated the hydrodynamics of the fed-batch system at various filling volumes, representing different stages of the fermentation process. The model was validated using experimental data of mixing time and mass transfer coefficient. The hydrodynamic model was then coupled with a Monod kinetic model of P. putida ‘s fermentation. Response surface methodology was used to generate a performance map of the pilot bioreactor at various aeration, agitation, and bioreactor filling volumes. The study considered different established scale-up approaches, such as constant tip speed and aeration rate across scales, constant

k_{L} a

, as well as constant power to unit of liquid volume (P/V). The performance of the bioreactor was assessed, and the optimum operating ranges of the input parameters were obtained at different stages of the fermentation. Using performance map the possibility of substrate and oxygen gradient formation, and the gradient severity inside the pilot bioreactor at different working volumes were evaluated.

本研究通过使用经过验证的计算流体动力学（CFD）模型，评估了从 2 升台式搅拌生物反应器到 200 升中试规模罐的假单胞菌给料批次发酵的放大。这种发酵工艺的一个主要问题是，随着规模的扩大，混合质量可能会降低，从而导致产量或产品质量下降。为了实现低风险放大，开发了一个多相欧拉-欧拉 CFD 模型，该模型模拟了代表发酵过程不同阶段的不同填充量下的进料批次系统的流体动力学。利用混合时间和传质系数的实验数据对模型进行了验证。然后将流体动力学模型与普氏菌发酵的莫诺动力学模型相结合。采用响应面方法生成了中试生物反应器在不同通气量、搅拌量和生物反应器填充量下的性能图。该研究考虑了不同的既定放大方法，如不同规模的恒定尖端速度和曝气速率、恒定 kLa 以及单位液体体积的恒定功率 (P/V)。对生物反应器的性能进行了评估，并得出了发酵不同阶段输入参数的最佳运行范围。利用性能图评估了基质和氧气梯度形成的可能性，以及不同工作容积下中试生物反应器内的梯度严重程度。

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引用次数: 0

Combined activated sludge and sand filtration for purification of UASB effluent with high suspended solids from water hyacinth juice 将活性污泥和砂滤结合起来，净化含有高悬浮固体的布袋莲汁污水

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2024-10-24 DOI: 10.1016/j.bej.2024.109540

Akinori Fujita , Mutsumi Sekine , Masatoshi Kishi , Tatsuki Toda

Rapid biogasification of water hyacinth, a globally overgrown species, using compression and up-flow anaerobic sludge blanket (UASB) treatment of the juice has recently been proposed. This study aimed to establish a post-purification system for UASB-treated juice to effectively utilize the remaining nutrients for valuable products, such as vegetables and microalgae. To easily remove hard-to-degrade suspended solids (SS) and organic matter from UASB-treated juice, the activated sludge (AS) and sand filtration processes—conventional biological treatments—were introduced, with a focus on their physical treatment properties. Over 76 % of SS and 43 % of the organic carbon were removed from UASB-treated juice during the process. The hydraulic retention time of the AS was reduced to one day, indicating the potential of minimizing the facility size. The light transmittance, which is crucial for microalgae mediums, improved sevenfold. Even after most of the SS was removed by the AS, the sand filtration further increased the light transmittance by 1.4 times. The AS effectively removed most of the SS and organic matter, whereas sand filtration enhanced the treatment stability and further improved the light transmittance. NH₄⁺ was mostly oxidized to NO₃^-, which is less toxic to plants. The total inorganic nitrogen content remained high after treatment, suggesting that UASB effluents from water hyacinth can be used as a nutrient source for hydroponics and microalgae cultivation in developing countries.

最近，有人提出利用压缩和上流式厌氧污泥毯（UASB）处理布袋莲（一种全球过度生长的物种）汁液，对其进行快速生物气化。本研究旨在为 UASB 处理过的果汁建立一个后净化系统，以有效利用剩余的营养物质，生产蔬菜和微藻等有价值的产品。为了从 UASB 处理过的果汁中轻松去除难以降解的悬浮固体（SS）和有机物，我们引入了活性污泥（AS）和砂滤工艺--传统的生物处理方法，重点关注其物理处理特性。在此过程中，UASB 处理过的果汁中 76% 以上的 SS 和 43% 的有机碳被去除。AS 的水力停留时间缩短到了一天，这表明可以最大限度地缩小设施规模。对微藻类培养基至关重要的透光率提高了七倍。即使在自动沉淀池去除大部分 SS 后，砂滤仍将透光率进一步提高了 1.4 倍。AS 有效地去除了大部分 SS 和有机物，而砂滤增强了处理的稳定性，并进一步提高了透光率。NH4+ 大多被氧化成对植物毒性较小的 NO3-。处理后的总无机氮含量仍然很高，这表明布袋莲产生的 UASB 污水可用作发展中国家水培和微藻栽培的营养源。

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引用次数: 0

Volemitol production from Yarrowia lipolytica via transaldolase gene (TAL) disruption and erythrose-4-phosphate (E4P) flux regulation 通过反式脱醛酶基因 (TAL) 干扰和赤藓糖-4-磷酸 (E4P) 通量调节从脂肪分解酵母菌中产生油醇

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2024-10-23 DOI: 10.1016/j.bej.2024.109535

Liyun Ji, Qing Li, Ye Li, Shuo Xu, Hairong Cheng

Volemitol (D-glycero-D-manno-Heptitol, C₇H₁₆O₇ with CAS No.488–38–0), a seven-carbon (C7) sugar-derived alcohol, has the potential to be used as a natural sweetener. The natural scarcity of sugar alcohols restricts their practical uses. However, Yarrowia lipolytica has shown significant promise in industrial production due to its capability to efficiently produce sugar alcohols like erythritol, D-threitol, mannitol, and xylitol by generating key biosynthetic intermediates through glycolysis and the phosphopentose (PPP) pathway. In this study, the transaldolase gene (TAL) in the erythritol synthesis pathway was knocked out in the erythritol-producing Y. lipolytica strain CGMCC7326 to inhibit erythritol production. TAL-deleted strain Y. lipolytica CGMCC7326ΔTAL exhibited a notable decline in erythritol production; however, a novel substance, volemitol, was generated from glucose at a titer of 50 g/L. Volemitol with 99 % purity was obtained as a white microneedle powder crystal through the enzymatic activity of mannitol dehydrogenase (MDH2), which reduces sedoheptulose to volemitol. The proposed biosynthetic pathway in Y. lipolytica CGMCC7326ΔTAL is: sedoheptulose-7-phosphate was converted to sedoheptulose, then was reduced to volemitol. In conclusion, this study is the first to report efficient volemitol production from glucose via fermentation by engineered Y. lipolytica.

沃尔米醇（D-甘油-D-甘露庚醇，C7H16O7，化学文摘社编号：488-38-0）是一种七碳（C7）糖醇，具有作为天然甜味剂的潜力。糖醇的天然稀缺性限制了其实际用途。然而，脂肪分解亚罗菌（Yarrowia lipolytica）通过糖酵解和磷酸戊糖（PPP）途径产生关键的生物合成中间体，能够高效地生产赤藓糖醇、D-苏力糖醇、甘露糖醇和木糖醇等糖醇，因而在工业生产中显示出巨大的前景。本研究敲除了生产赤藓糖醇的脂溶性酵母菌株 CGMCC7326 中赤藓糖醇合成途径中的反醛醇酶基因（TAL），以抑制赤藓糖醇的生产。TAL缺失菌株 Y. lipolytica CGMCC7326ΔTAL 的赤藓糖醇产量明显下降，但却以 50 克/升的滴度从葡萄糖中生成了一种新物质--伏来米醇。通过甘露醇脱氢酶（MDH2）的酶活性，将沉淀七聚糖还原为油菜醇，从而获得纯度为 99% 的白色微针粉末晶体。所提出的 Y. lipolytica CGMCC7326ΔTAL 的生物合成途径是：7-磷酸沉降色酮糖被转化为沉降色酮糖，然后被还原为 volemitol。总之，本研究首次报道了工程脂溶性酵母通过发酵从葡萄糖中高效生产出伏来米醇。

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引用次数: 0

Enhanced antimicrobial performance of single-use polyamide 56 nanofiber membranes modified with chitosan, reactive dyes, and poly(hexamethylene biguanide) (PHMB) for biological waste treatments 用壳聚糖、活性染料和聚六亚甲基双胍 (PHMB) 改性的一次性聚酰胺 56 纳米纤维膜的抗菌性能增强，可用于生物废物处理

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2024-10-22 DOI: 10.1016/j.bej.2024.109538

Jia-Hong Yang , Quang-Vinh Le , Bing-Lan Liu , Penjit Srinophakun , Chi-Yun Wang , Chen‑Yaw Chiu , I-Son Ng , Kuei-Hsiang Chen , Yu-Kaung Chang

Polyamide 56 (PA56), derived from renewable resources like wheat and corn via microbial fermentation, presents a sustainable alternative to traditional petrochemical-based polymers and has gained attention for its versatility in various fields. This study explores the antibacterial properties of bio-nylon nanofibers through fabrication and modification processes. PA56 nanofiber membrane was initially fabricated using electrospinning technology. These membranes were then modified with chitosan (CS), forming PA56-CS membranes, and further enhanced with Reactive dyes (Reactive Green 19, RG19 and Reactive Red 141, RR141) to produce PA56-CS-DYE membranes. Poly(hexamethylene biguanide) (PHMB), a positively charged polymer, was subsequently bonded to these membranes, resulting in PA56-CS-DYE-PHMB nanofiber membranes. Comprehensive physical characterizations using Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and thermogravimetric analysis (TGA) were conducted alongside qualitative and quantitative antibacterial assays against E. coli. The PA56-CS-RR141-PHMB nanofiber membrane exhibited exceptional antibacterial efficiency (AE), exceeding 97 %, indicating strong potential for biological waste treatment and related applications. However, the high antibacterial performance of these membranes is primarily suited for single-use systems, as their efficacy diminishes with repeated use, making them ideal for immediate and short-term applications in healthcare and environmental settings.

聚酰胺 56（PA56）通过微生物发酵从小麦和玉米等可再生资源中提取，是传统石化基聚合物的可持续替代品，因其在各个领域的多功能性而备受关注。本研究通过制备和改性工艺探索了生物尼龙纳米纤维的抗菌特性。PA56 纳米纤维膜最初采用电纺丝技术制造。然后用壳聚糖（CS）对这些膜进行改性，形成 PA56-CS 膜，再用活性染料（活性绿 19、RG19 和活性红 141、RR141）进一步增强，制成 PA56-CS-DYE 膜。带正电荷的聚合物聚六亚甲基双胍（PHMB）随后被粘合到这些膜上，形成 PA56-CS-DYE-PHMB 纳米纤维膜。利用傅立叶变换红外光谱（FTIR）、扫描电子显微镜（SEM）和热重分析（TGA）进行了全面的物理表征，并对大肠杆菌进行了定性和定量的抗菌检测。PA56-CS-RR141-PHMB 纳米纤维膜表现出卓越的抗菌效率（AE），超过 97%，显示出其在生物废物处理和相关应用方面的巨大潜力。不过，这些膜的高抗菌性能主要适用于一次性使用系统，因为它们的功效会随着重复使用而降低，因此非常适合医疗保健和环境领域的即时和短期应用。

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引用次数: 0

Recent trends in metabolic engineering for microbial production of value-added natural products 微生物生产增值天然产品的代谢工程最新趋势

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal

Pub Date : 2024-10-21 DOI: 10.1016/j.bej.2024.109537

Prasenjit Chakraborty , Randhir Kumar , Sanjay Karn , Payal Patel , Haren Gosai

Microbe-mediated chemical production is a sustainable approach for production of natural products that are important for our society. Traditional fermentation production of natural products viz. drugs, nutraceuticals, pigments, aromatic compounds, and biofuels has limitations such as low yield, high cost and time- consuming process. Significant success in the economical production of these natural products has been established by altering the metabolic pathways of microorganisms through metabolic engineering. Recent advancements in new tools and strategies have led to a significant reduction in the turnaround time in the classic approach i.e. design-build-test-learn (DBTL) cycle in metabolic engineering. Here, we summarize various tools and strategies developed in the area of metabolic engineering with a focus on value-added natural products Also, this review lists bioprospecting of microorganisms through metabolic engineering in last five years for production of natural products. At the end, outlook, challenges and opportunities for the successful establishment of robust engineered microbial cell factories have been reported.

以微生物为媒介的化学生产是一种生产对社会十分重要的天然产品的可持续方法。药物、营养保健品、色素、芳香化合物和生物燃料等天然产品的传统发酵生产具有产量低、成本高和耗时长等局限性。通过代谢工程改变微生物的代谢途径，在经济生产这些天然产品方面取得了巨大成功。新工具和新策略的最新进展大大缩短了传统方法（即代谢工程中的设计-构建-测试-学习（DBTL）循环）的周转时间。在此，我们总结了代谢工程领域开发的各种工具和策略，重点关注增值天然产品。此外，本综述还列举了过去五年中通过代谢工程对微生物进行生物勘探以生产天然产品的情况。最后，报告了成功建立强大的工程微生物细胞工厂的前景、挑战和机遇。

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引用次数: 0